Cp4.1LG14g05600 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG14g05600 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 551/744 (74.06%), Postives = 645/744 (86.69%), Query Frame = 0

Query: 27  KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAAR 86
           KTYV+ MD+SAMP  +++HLQWYS+ + SV  +  +E   G   RI+Y+YQ  FHG+AA+
Sbjct: 35  KTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE-EEGNNNRILYTYQTAFHGLAAQ 94

Query: 87  LSEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVL 146
           L++EEAERLEEEDGV+A+ PET+YELHTTRSP FLGLE  +S   W++++ DHDV+VGVL
Sbjct: 95  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 154

Query: 147 DTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKIVGARMFYHGYEAATGKFN 206
           DTGIWPESESFND GMSPVPA W+G CETG+ F K+NCNRKIVGAR+FY GYEAATGK +
Sbjct: 155 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 214

Query: 207 EHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGC 266
           E LEYKSPRD+DGHGTHTAATVAGSP  GANL G+AYGTARGMA  AR+AAYKVCW GGC
Sbjct: 215 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 274

Query: 267 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPD 326
           FSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGN GPD
Sbjct: 275 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 334

Query: 327 PVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPENKQFPVVYMGS 386
           P+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLYKGR  +P+NKQ+P+VY+G 
Sbjct: 335 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 394

Query: 387 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 446
           N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGG+GM+L NTA NGE
Sbjct: 395 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 454

Query: 447 ELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATLGFLGTRLGVRPSPVVAAFSSRGPN 506
           ELVAD H++PAVAVGE+EGK IK YA+T++K TA+L  LGTR+G++PSPVVAAFSSRGPN
Sbjct: 455 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 514

Query: 507 FLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALI 566
           FL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVAALI
Sbjct: 515 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 574

Query: 567 KSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKALDPGLV 626
           KS+HPDWSP+AIKSALMTTAYVHDN +KPL D+SGA+PSSPYDHGAGHI+P +A DPGLV
Sbjct: 575 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 634

Query: 627 YEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHH-LLPNPGDLNYPAISAVFPEKTSVT 686
           Y+I PQ+YFEFLCTQDL+PSQLKVF+K+SNR+C H L  NPG+LNYPAISA+FPE T V 
Sbjct: 635 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 694

Query: 687 SLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTRRYQKLSYKITFLTKKRQSM 746
           ++TL RTVTNVGP  SSY  +V+PF GA+V V+P++LNFT ++QKLSY +TF T+ R   
Sbjct: 695 AMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR 754

Query: 747 PEFGGLIWKDGTHSVRSPIVITWL 770
           PEFGGL+WK  TH VRSP++ITWL
Sbjct: 755 PEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Cp4.1LG14g05600 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 3.2e-232
Identity = 410/760 (53.95%), Postives = 529/760 (69.61%), Query Frame = 0

Query: 11  LLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQ 70
           LL +  C + SS+    TY+V M +S MP SF  H  WY + L S+  + E         
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE--------- 73

Query: 71  RIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADSNS 130
            ++Y+Y+N  HG + RL++EEA+ L  + GV+++ PE +YELHTTR+P FLGL+   ++ 
Sbjct: 74  -LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD- 133

Query: 131 AWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKIVG 190
            + +  +  DV+VGVLDTG+WPES+S++D G  P+P+ WKG CE G +F+   CNRK++G
Sbjct: 134 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 193

Query: 191 ARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMA 250
           AR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS   GA+LLGYA GTARGMA
Sbjct: 194 ARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 253

Query: 251 PGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 310
           P AR+A YKVCW GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AM
Sbjct: 254 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAM 313

Query: 311 EMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGR 370
           E G+ VSCSAGNAGP   SL+NV+PWITTVGA T+DRDFPA+  LGNG+  TGVSL+KG 
Sbjct: 314 ERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE 373

Query: 371 ITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 430
             +P+ K  P +Y G N+SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK 
Sbjct: 374 -ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 433

Query: 431 AGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATLGFLGTRLG 490
           AGGVGMILANTAANGEELVAD HL+PA  VGE+ G  I+ Y  T+  PTA++  LGT +G
Sbjct: 434 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 493

Query: 491 VRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 550
           V+PSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+S
Sbjct: 494 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 553

Query: 551 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDH 610
           GTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+DH
Sbjct: 554 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 613

Query: 611 GAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNPGDLN 670
           GAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+ +         +  DLN
Sbjct: 614 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLN 673

Query: 671 YPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTRRYQK 730
           YP+ +        V +    RTVT+VG A +      +   G  + VEP  LNF    +K
Sbjct: 674 YPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 733

Query: 731 LSYKITFL--TKKRQSMPEFGGLIWKDGTHSVRSPIVITW 769
            SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 734 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG14g05600 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 2.4e-219
Identity = 394/768 (51.30%), Postives = 516/768 (67.19%), Query Frame = 0

Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           + ++  F  LLL +    KTY+++++ S  P+SF  H  WY++ L S             
Sbjct: 11  ITIITTFLFLLLHTTA-KKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------- 70

Query: 69  EQRIIYSYQNVFHGVAARLSEEEAER-LEEEDGVLAIFPETKYELHTTRSPRFLGLEPAD 128
           E  ++Y+Y   FHG +A L   EA+  L   + +L IF +  Y LHTTR+P FLGL    
Sbjct: 71  ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--S 130

Query: 129 SNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRK 188
                    + + VI+GVLDTG+WPES SF+D  M  +P+ WKGECE+G DF  + CN+K
Sbjct: 131 EFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKK 190

Query: 189 IVGARMFYHGYEAAT-GKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTA 248
           ++GAR F  G++ A+ G F+   E  SPRD DGHGTHT+ T AGS    A+ LGYA GTA
Sbjct: 191 LIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTA 250

Query: 249 RGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAA 308
           RGMA  AR+A YKVCW+ GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ A
Sbjct: 251 RGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGA 310

Query: 309 FGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSL 368
           F AME GVFVSCSAGN+GP   S+ NV+PW+ TVGA T+DRDFPA   LGNG+ +TGVSL
Sbjct: 311 FSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSL 370

Query: 369 YKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGV 428
           Y G       K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG 
Sbjct: 371 YSG--VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 430

Query: 429 VVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATLGFLG 488
           VV++AGG+GMI+ANTAA+GEELVAD HL+PA+AVG++ G  ++ Y  ++ KPTA L F G
Sbjct: 431 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKG 490

Query: 489 TRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKF 548
           T L V+PSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +F
Sbjct: 491 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 550

Query: 549 NILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSS 608
           NI+SGTSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+
Sbjct: 551 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 610

Query: 609 PYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNP 668
           PY HG+GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C     +P
Sbjct: 611 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP 670

Query: 669 GDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTR 728
           G LNYP+ S +F  K  V      R VTNVG A+S Y   V       + V+P  L+F  
Sbjct: 671 GQLNYPSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 730

Query: 729 RYQKLSYKITFLTKKRQSM---PEFGGLIWKDGTHSVRSPIVITWLSF 772
             +K  Y +TF++KK  SM    EFG + W +  H VRSP+  +W  F
Sbjct: 731 VGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Cp4.1LG14g05600 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.9e-216
Identity = 384/758 (50.66%), Postives = 513/758 (67.68%), Query Frame = 0

Query: 28  TYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAARL 87
           TY+V +D  A P  F  H  WY++ L S+  +P           II++Y  VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  SEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++A +L +   V+++ PE    LHTTRSP FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   +DF +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 HLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGCF 267
             E++SPRD DGHGTHTA+  AG     A+ LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGN GP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPENKQFPVVYMGS- 387
           +++TNV+PW+TTVGA T+DRDFPA VKLGNG+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 447
              +   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI+AN   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAVGEREGKAIKVY------ALTNRKPTATLGFLGTRLGVRPSPVVAAF 507
            LVADCH++PA +VG   G  I+ Y      + +++ PTAT+ F GTRLG+RP+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKY-----SNRSCHHLLPNPGDLNYPAIS 687
           +DPGLVY+I   DY  FLC  + T + +   ++        R   H+    G+LNYP+ S
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV----GNLNYPSFS 686

Query: 688 AVFPE-KTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTRRYQKLSYK 747
            VF +   S  S    RTVTNVG + S Y   + P  G  V VEPE L+F R  QKLS+ 
Sbjct: 687 VVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFV 746

Query: 748 ITFLTKKRQSMP-----EFGGLIWKDGTHSVRSPIVIT 768
           +   T + +  P     E G ++W DG  +V SP+V+T
Sbjct: 747 VRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cp4.1LG14g05600 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 1.3e-214
Identity = 393/772 (50.91%), Postives = 516/772 (66.84%), Query Frame = 0

Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           L L L F  +  +++Q  KT++ ++D  +MP  F  H  WYST                 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  EQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADS 128
           E RI++ Y  VFHG +A ++ +EA+ L     VLA+F + + ELHTTRSP+FLGL+  + 
Sbjct: 68  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQ 127

Query: 129 NSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKI 188
              WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  FS +NCNRKI
Sbjct: 128 KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 187

Query: 189 VGARMFYHGYEAAT-GKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTAR 248
           +GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     A++ GYA G A+
Sbjct: 188 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 247

Query: 249 GMAPGARIAAYKVCWA-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLS 308
           G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D ++
Sbjct: 248 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 307

Query: 309 VAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTG 368
           + ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LG+G  + G
Sbjct: 308 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 367

Query: 369 VSLYKGRITIPEN-KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRV 428
           VSLY G   +P N + FPVVY G   S    +SLC+E TLDP  V GKIVICDRG SPRV
Sbjct: 368 VSLYAG---VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 427

Query: 429 QKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATL 488
            KG+VVK AGGVGMILAN A+NGE LV D HL+PA AVG  EG  IK YA ++  P A++
Sbjct: 428 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 487

Query: 489 GFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTR 548
            F GT +G++P+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D R
Sbjct: 488 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 547

Query: 549 RVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGA 608
           + +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S  
Sbjct: 548 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 607

Query: 609 SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHL 668
             ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R     
Sbjct: 608 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTR 667

Query: 669 LPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPES 728
            P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G  V V+P  
Sbjct: 668 KPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPR 727

Query: 729 LNFTRRYQKLSYKITFLTKKR-----QSMPEFGGLIWKD-GTHSVRSPIVIT 768
           L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of Cp4.1LG14g05600 vs. NCBI nr
Match: XP_023552616.1 (subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1555 bits (4025), Expect = 0.0
Identity = 772/772 (100.00%), Postives = 772/772 (100.00%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Cp4.1LG14g05600 vs. NCBI nr
Match: KAG6576764.1 (Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1545 bits (4001), Expect = 0.0
Identity = 766/772 (99.22%), Postives = 769/772 (99.61%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           M HTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MGHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARLSE+EAERLEEEDGVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHA VTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Cp4.1LG14g05600 vs. NCBI nr
Match: XP_022922929.1 (subtilisin-like protease SBT1.3 [Cucurbita moschata])

HSP 1 Score: 1543 bits (3994), Expect = 0.0
Identity = 765/772 (99.09%), Postives = 770/772 (99.74%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARL+EEEAERLEEE+GVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIK+YALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHA VTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Cp4.1LG14g05600 vs. NCBI nr
Match: XP_022984431.1 (subtilisin-like protease SBT1.3 [Cucurbita maxima])

HSP 1 Score: 1531 bits (3963), Expect = 0.0
Identity = 759/772 (98.32%), Postives = 764/772 (98.96%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDC LLSSAQF+KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKY+LHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSPTA ANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           G SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVGPATSSYHA  TPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Cp4.1LG14g05600 vs. NCBI nr
Match: KAG7014803.1 (Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1521 bits (3938), Expect = 0.0
Identity = 754/767 (98.31%), Postives = 759/767 (98.96%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLL SSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLHSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARLSE+EAERLEEEDGVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEDEAERLEEEDGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180
           LGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGA+MFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGAKMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGR TIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRTTIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHA VTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVIT 767
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVR    +T
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRITFAVT 767

BLAST of Cp4.1LG14g05600 vs. ExPASy TrEMBL
Match: A0A6J1E4V2 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE=3 SV=1)

HSP 1 Score: 1543 bits (3994), Expect = 0.0
Identity = 765/772 (99.09%), Postives = 770/772 (99.74%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARL+EEEAERLEEE+GVLAIFPETKYELHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSPTAGANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPE+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIK+YALTNRKPTA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRKPTA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHA VTPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Cp4.1LG14g05600 vs. ExPASy TrEMBL
Match: A0A6J1J576 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3 SV=1)

HSP 1 Score: 1531 bits (3963), Expect = 0.0
Identity = 759/772 (98.32%), Postives = 764/772 (98.96%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MAHTPLKWLCLLLVFDC LLSSAQF+KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60

Query: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRF 120
           EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKY+LHTTRSPRF
Sbjct: 61  EREGNGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRSPRF 120

Query: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFS 180
           LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF+
Sbjct: 121 LGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFT 180

Query: 181 KQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLG 240
           KQNCNRKIVGARMFYHGYEAATGKFNE LEYKSPRDQDGHGTHTAATVAGSPTA ANLLG
Sbjct: 181 KQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASANLLG 240

Query: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300
           YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD
Sbjct: 241 YAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRD 300

Query: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360
           SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT
Sbjct: 301 SLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRT 360

Query: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420
           VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP
Sbjct: 361 VTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 420

Query: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTA 480
           RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRK TA
Sbjct: 421 RVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKATA 480

Query: 481 TLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540
           +LGFLGT+LGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD
Sbjct: 481 SLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTD 540

Query: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600
           TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS
Sbjct: 541 TRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSS 600

Query: 601 GASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660
           G SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH
Sbjct: 601 GTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCH 660

Query: 661 HLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPE 720
           HLLPNPGDLNYPAISAVFPEK SVTSLTLHRTVTNVGPATSSYHA  TPFNGAAVKVEPE
Sbjct: 661 HLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKVEPE 720

Query: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772
           SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV
Sbjct: 721 SLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772

BLAST of Cp4.1LG14g05600 vs. ExPASy TrEMBL
Match: A0A6J1FZU3 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE=3 SV=1)

HSP 1 Score: 1450 bits (3753), Expect = 0.0
Identity = 715/773 (92.50%), Postives = 744/773 (96.25%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVV-N 60
           MAHTPL+WLC  L F CL  SSAQF KTYVVQMDRSAMPDSFSDHL+WYSTV++SVVV N
Sbjct: 1   MAHTPLQWLCFPLFFHCLFFSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PEREG--NGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRS 120
           PEREG  NGGGE RIIYSYQNVFHGVAARLSEEEAERLEEE GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPA+WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 DFSKQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGAN 240
            F+KQNCNRKIVGAR+FYHGYEAATGKFN+ LEYKSPRDQDGHGTHTAATVAGSP AGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTVTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLY+GRITI ENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRK 480
           ISPRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHL+PAVAVGE EGKAIK YALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLG LGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNR 660
           D+S ASPSSPY+HGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTPSQLKVFSK+SNR
Sbjct: 601 DASAASPSSPYEHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKV 720
           SCH LLPNPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVGPA+SSYHA V+PF GAAVK+
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLS 770
           EPE LNFTRRYQKLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPI+ITWLS
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773

BLAST of Cp4.1LG14g05600 vs. ExPASy TrEMBL
Match: A0A6J1HS88 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111467351 PE=3 SV=1)

HSP 1 Score: 1440 bits (3728), Expect = 0.0
Identity = 714/773 (92.37%), Postives = 742/773 (95.99%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVV-N 60
           MAHTPL+WLCL L F C LLSSAQF  TYVVQMDRSAMPDSFSD  +WYSTVL+SVVV N
Sbjct: 1   MAHTPLQWLCLPLFFHCFLLSSAQFFNTYVVQMDRSAMPDSFSDPFEWYSTVLSSVVVDN 60

Query: 61  PEREG--NGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRS 120
           PEREG  NGGGE RIIYSYQNVFHGVAARLSEEEAER+EEE GVLA+FPE KYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERVEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPA+WKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 DFSKQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGAN 240
            F+KQNCNRKIVGAR+FYHGYEAATGKFN+ LEYKSPRDQDGHGTHTAATVAGSP AGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTVTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLY+GRITI ENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRK 480
           ISPRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHL+PAVAVGE EGKAIK YALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATL  LGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLEILGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLR 600

Query: 601 DSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNR 660
           D+S ASPSSPYDHGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTPSQLKVFSK+SNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKV 720
           SC+ LL NPGDLNYPAISAVFPEKT+VTSLTLHRTVTNVGPA+SSYHA V+PFNGAAVKV
Sbjct: 661 SCNRLLLNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFNGAAVKV 720

Query: 721 EPESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLS 770
           EPE LNFTRRYQKLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPI+ITWLS
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773

BLAST of Cp4.1LG14g05600 vs. ExPASy TrEMBL
Match: A0A5A7SUB4 (Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00040 PE=3 SV=1)

HSP 1 Score: 1432 bits (3706), Expect = 0.0
Identity = 706/773 (91.33%), Postives = 744/773 (96.25%), Query Frame = 0

Query: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNP 60
           MA +P+K LCLLL  D LLLSSA F+KTYVVQMDRSAMP SF++H +WYS VL++VVV+ 
Sbjct: 1   MADSPIKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  EREGNGGG-EQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPR 120
           EREGNGGG E+RIIYSYQNVFHGVAARLSEEE E+LEEEDGV+AIFPE KYELHTTRSPR
Sbjct: 61  EREGNGGGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRSPR 120

Query: 121 FLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDF 180
           FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPES+SF+DAGMSPVPAHWKGECETGR F
Sbjct: 121 FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGRGF 180

Query: 181 SKQNCNRKIVGARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLL 240
           +KQNCNRKIVGAR+FYHGY+AATGKFNE LEYKSPRDQDGHGTHTAATVAGSP AGA+LL
Sbjct: 181 TKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGASLL 240

Query: 241 GYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300
           GYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR
Sbjct: 241 GYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYR 300

Query: 301 DSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGR 360
           DSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+GR
Sbjct: 301 DSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGDGR 360

Query: 361 TVTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGIS 420
           T++G SLY+GRITIPENKQFP+VYMGSNSS+PDPSSLCLEGTLDPH VAGKIVICDRGIS
Sbjct: 361 TMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRGIS 420

Query: 421 PRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPT 480
           PRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHLVPAVAVGE+EGKAIK YALTNRK T
Sbjct: 421 PRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRKAT 480

Query: 481 ATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540
           ATLGFLGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT
Sbjct: 481 ATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTT 540

Query: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDS 600
           DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+DS
Sbjct: 541 DTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQDS 600

Query: 601 SGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSC 660
           S ASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYF+FLCTQDLTP+QLKVFSKYSNR+C
Sbjct: 601 SAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNRTC 660

Query: 661 HHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEP 720
             LLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHA VTPF GAAVKVEP
Sbjct: 661 RRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKVEP 720

Query: 721 ESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLSFV 772
           ESLNFTRRY+KLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPIVITWLSFV
Sbjct: 721 ESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVITWLSFV 773

BLAST of Cp4.1LG14g05600 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 1155.6 bits (2988), Expect = 0.0e+00
Identity = 551/744 (74.06%), Postives = 645/744 (86.69%), Query Frame = 0

Query: 27  KTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAAR 86
           KTYV+ MD+SAMP  +++HLQWYS+ + SV  +  +E   G   RI+Y+YQ  FHG+AA+
Sbjct: 35  KTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE-EEGNNNRILYTYQTAFHGLAAQ 94

Query: 87  LSEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVL 146
           L++EEAERLEEEDGV+A+ PET+YELHTTRSP FLGLE  +S   W++++ DHDV+VGVL
Sbjct: 95  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 154

Query: 147 DTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKIVGARMFYHGYEAATGKFN 206
           DTGIWPESESFND GMSPVPA W+G CETG+ F K+NCNRKIVGAR+FY GYEAATGK +
Sbjct: 155 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 214

Query: 207 EHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGC 266
           E LEYKSPRD+DGHGTHTAATVAGSP  GANL G+AYGTARGMA  AR+AAYKVCW GGC
Sbjct: 215 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 274

Query: 267 FSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPD 326
           FSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGN GPD
Sbjct: 275 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 334

Query: 327 PVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPENKQFPVVYMGS 386
           P+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLYKGR  +P+NKQ+P+VY+G 
Sbjct: 335 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 394

Query: 387 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 446
           N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGG+GM+L NTA NGE
Sbjct: 395 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 454

Query: 447 ELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATLGFLGTRLGVRPSPVVAAFSSRGPN 506
           ELVAD H++PAVAVGE+EGK IK YA+T++K TA+L  LGTR+G++PSPVVAAFSSRGPN
Sbjct: 455 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 514

Query: 507 FLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALI 566
           FL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVAALI
Sbjct: 515 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 574

Query: 567 KSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKALDPGLV 626
           KS+HPDWSP+AIKSALMTTAYVHDN +KPL D+SGA+PSSPYDHGAGHI+P +A DPGLV
Sbjct: 575 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 634

Query: 627 YEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHH-LLPNPGDLNYPAISAVFPEKTSVT 686
           Y+I PQ+YFEFLCTQDL+PSQLKVF+K+SNR+C H L  NPG+LNYPAISA+FPE T V 
Sbjct: 635 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 694

Query: 687 SLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTRRYQKLSYKITFLTKKRQSM 746
           ++TL RTVTNVGP  SSY  +V+PF GA+V V+P++LNFT ++QKLSY +TF T+ R   
Sbjct: 695 AMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR 754

Query: 747 PEFGGLIWKDGTHSVRSPIVITWL 770
           PEFGGL+WK  TH VRSP++ITWL
Sbjct: 755 PEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Cp4.1LG14g05600 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 806.2 bits (2081), Expect = 2.3e-233
Identity = 410/760 (53.95%), Postives = 529/760 (69.61%), Query Frame = 0

Query: 11  LLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQ 70
           LL +  C + SS+    TY+V M +S MP SF  H  WY + L S+  + E         
Sbjct: 14  LLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE--------- 73

Query: 71  RIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADSNS 130
            ++Y+Y+N  HG + RL++EEA+ L  + GV+++ PE +YELHTTR+P FLGL+   ++ 
Sbjct: 74  -LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD- 133

Query: 131 AWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKIVG 190
            + +  +  DV+VGVLDTG+WPES+S++D G  P+P+ WKG CE G +F+   CNRK++G
Sbjct: 134 LFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIG 193

Query: 191 ARMFYHGYEAATGKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMA 250
           AR F  GYE+  G  +E  E +SPRD DGHGTHT++T AGS   GA+LLGYA GTARGMA
Sbjct: 194 ARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 253

Query: 251 PGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAM 310
           P AR+A YKVCW GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AM
Sbjct: 254 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAM 313

Query: 311 EMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGR 370
           E G+ VSCSAGNAGP   SL+NV+PWITTVGA T+DRDFPA+  LGNG+  TGVSL+KG 
Sbjct: 314 ERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGE 373

Query: 371 ITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKN 430
             +P+ K  P +Y G N+SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK 
Sbjct: 374 -ALPD-KLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKA 433

Query: 431 AGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATLGFLGTRLG 490
           AGGVGMILANTAANGEELVAD HL+PA  VGE+ G  I+ Y  T+  PTA++  LGT +G
Sbjct: 434 AGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG 493

Query: 491 VRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILS 550
           V+PSPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+S
Sbjct: 494 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 553

Query: 551 GTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDH 610
           GTSMSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+DH
Sbjct: 554 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 613

Query: 611 GAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNPGDLN 670
           GAGH++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+ +         +  DLN
Sbjct: 614 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLN 673

Query: 671 YPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTRRYQK 730
           YP+ +        V +    RTVT+VG A +      +   G  + VEP  LNF    +K
Sbjct: 674 YPSFAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEK 733

Query: 731 LSYKITFL--TKKRQSMPEFGGLIWKDGTHSVRSPIVITW 769
            SY +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 734 KSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG14g05600 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 763.5 bits (1970), Expect = 1.7e-220
Identity = 394/768 (51.30%), Postives = 516/768 (67.19%), Query Frame = 0

Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           + ++  F  LLL +    KTY+++++ S  P+SF  H  WY++ L S             
Sbjct: 11  ITIITTFLFLLLHTTA-KKTYIIRVNHSDKPESFLTHHDWYTSQLNS------------- 70

Query: 69  EQRIIYSYQNVFHGVAARLSEEEAER-LEEEDGVLAIFPETKYELHTTRSPRFLGLEPAD 128
           E  ++Y+Y   FHG +A L   EA+  L   + +L IF +  Y LHTTR+P FLGL    
Sbjct: 71  ESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--S 130

Query: 129 SNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRK 188
                    + + VI+GVLDTG+WPES SF+D  M  +P+ WKGECE+G DF  + CN+K
Sbjct: 131 EFGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKK 190

Query: 189 IVGARMFYHGYEAAT-GKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTA 248
           ++GAR F  G++ A+ G F+   E  SPRD DGHGTHT+ T AGS    A+ LGYA GTA
Sbjct: 191 LIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTA 250

Query: 249 RGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAA 308
           RGMA  AR+A YKVCW+ GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ A
Sbjct: 251 RGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGA 310

Query: 309 FGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSL 368
           F AME GVFVSCSAGN+GP   S+ NV+PW+ TVGA T+DRDFPA   LGNG+ +TGVSL
Sbjct: 311 FSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSL 370

Query: 369 YKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGV 428
           Y G       K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG 
Sbjct: 371 YSG--VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGA 430

Query: 429 VVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATLGFLG 488
           VV++AGG+GMI+ANTAA+GEELVAD HL+PA+AVG++ G  ++ Y  ++ KPTA L F G
Sbjct: 431 VVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKG 490

Query: 489 TRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKF 548
           T L V+PSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +F
Sbjct: 491 TVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQF 550

Query: 549 NILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSS 608
           NI+SGTSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL D++  S S+
Sbjct: 551 NIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSN 610

Query: 609 PYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHLLPNP 668
           PY HG+GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C     +P
Sbjct: 611 PYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP 670

Query: 669 GDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTR 728
           G LNYP+ S +F  K  V      R VTNVG A+S Y   V       + V+P  L+F  
Sbjct: 671 GQLNYPSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKS 730

Query: 729 RYQKLSYKITFLTKKRQSM---PEFGGLIWKDGTHSVRSPIVITWLSF 772
             +K  Y +TF++KK  SM    EFG + W +  H VRSP+  +W  F
Sbjct: 731 VGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNRF 754

BLAST of Cp4.1LG14g05600 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 753.8 bits (1945), Expect = 1.3e-217
Identity = 384/758 (50.66%), Postives = 513/758 (67.68%), Query Frame = 0

Query: 28  TYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGGEQRIIYSYQNVFHGVAARL 87
           TY+V +D  A P  F  H  WY++ L S+  +P           II++Y  VFHG +ARL
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------PSIIHTYDTVFHGFSARL 86

Query: 88  SEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADSNSAWSQQIADHDVIVGVLD 147
           + ++A +L +   V+++ PE    LHTTRSP FLGL   D      +     D+++GV+D
Sbjct: 87  TSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVID 146

Query: 148 TGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKIVGARMFYHGYEAATGKFNE 207
           TG+WPE  SF+D G+ PVP  WKG+C   +DF +  CNRK+VGAR F  GYEA  GK NE
Sbjct: 147 TGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE 206

Query: 208 HLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTARGMAPGARIAAYKVCWAGGCF 267
             E++SPRD DGHGTHTA+  AG     A+ LGYA+G A GMAP AR+AAYKVCW  GC+
Sbjct: 207 TTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCY 266

Query: 268 SSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNAGPDP 327
            SDIL+A D AVADGV+V+S+S+GG V  YY D++++ AFGA++ G+FVS SAGN GP  
Sbjct: 267 DSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGA 326

Query: 328 VSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTGVSLYKGRITIPENKQFPVVYMGS- 387
           +++TNV+PW+TTVGA T+DRDFPA VKLGNG+ ++GVS+Y G    P  + +P+VY GS 
Sbjct: 327 LTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDP-GRMYPLVYGGSL 386

Query: 388 NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGVGMILANTAANGE 447
              +   SSLCLEG+LDP+ V GKIV+CDRGI+ R  KG +V+  GG+GMI+AN   +GE
Sbjct: 387 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGE 446

Query: 448 ELVADCHLVPAVAVGEREGKAIKVY------ALTNRKPTATLGFLGTRLGVRPSPVVAAF 507
            LVADCH++PA +VG   G  I+ Y      + +++ PTAT+ F GTRLG+RP+PVVA+F
Sbjct: 447 GLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASF 506

Query: 508 SSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVS 567
           S+RGPN  T EILKPD++APG+NILAAW  + GPS +T+D RR +FNILSGTSM+CPHVS
Sbjct: 507 SARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVS 566

Query: 568 GVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGASPSSPYDHGAGHINPRKA 627
           G+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +P+ D S  + SS  D+G+GH++P KA
Sbjct: 567 GLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKA 626

Query: 628 LDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKY-----SNRSCHHLLPNPGDLNYPAIS 687
           +DPGLVY+I   DY  FLC  + T + +   ++        R   H+    G+LNYP+ S
Sbjct: 627 MDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV----GNLNYPSFS 686

Query: 688 AVFPE-KTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPESLNFTRRYQKLSYK 747
            VF +   S  S    RTVTNVG + S Y   + P  G  V VEPE L+F R  QKLS+ 
Sbjct: 687 VVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFV 746

Query: 748 ITFLTKKRQSMP-----EFGGLIWKDGTHSVRSPIVIT 768
           +   T + +  P     E G ++W DG  +V SP+V+T
Sbjct: 747 VRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cp4.1LG14g05600 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 747.7 bits (1929), Expect = 9.6e-216
Identity = 393/772 (50.91%), Postives = 516/772 (66.84%), Query Frame = 0

Query: 9   LCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVVNPEREGNGGG 68
           L L L F  +  +++Q  KT++ ++D  +MP  F  H  WYST                 
Sbjct: 8   LLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE------------- 67

Query: 69  EQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYELHTTRSPRFLGLEPADS 128
           E RI++ Y  VFHG +A ++ +EA+ L     VLA+F + + ELHTTRSP+FLGL+  + 
Sbjct: 68  ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQ 127

Query: 129 NSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGRDFSKQNCNRKI 188
              WS+     DVI+GV DTGIWPE  SF+D  + P+P  W+G CE+G  FS +NCNRKI
Sbjct: 128 KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 187

Query: 189 VGARMFYHGYEAAT-GKFNEHLEYKSPRDQDGHGTHTAATVAGSPTAGANLLGYAYGTAR 248
           +GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     A++ GYA G A+
Sbjct: 188 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 247

Query: 249 GMAPGARIAAYKVCWA-GGCFSSDILSAVDRAVADGVNVLSISLGGG---VSSYYRDSLS 308
           G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG    S YY D ++
Sbjct: 248 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 307

Query: 309 VAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTVTG 368
           + ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA   LG+G  + G
Sbjct: 308 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 367

Query: 369 VSLYKGRITIPEN-KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRV 428
           VSLY G   +P N + FPVVY G   S    +SLC+E TLDP  V GKIVICDRG SPRV
Sbjct: 368 VSLYAG---VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRV 427

Query: 429 QKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRKPTATL 488
            KG+VVK AGGVGMILAN A+NGE LV D HL+PA AVG  EG  IK YA ++  P A++
Sbjct: 428 AKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASI 487

Query: 489 GFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTR 548
            F GT +G++P+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   GP+ L +D R
Sbjct: 488 DFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPR 547

Query: 549 RVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDSSGA 608
           + +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+ + L D S  
Sbjct: 548 KTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTG 607

Query: 609 SPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNRSCHHL 668
             ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V ++   R     
Sbjct: 608 KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTR 667

Query: 669 LPNPGDLNYPAISAVFP-EKTSVTSLTLHRTVTNVGPATSSYHAAVTPFNGAAVKVEPES 728
            P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    G  V V+P  
Sbjct: 668 KPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPR 727

Query: 729 LNFTRRYQKLSYKITFLTKKR-----QSMPEFGGLIWKD-GTHSVRSPIVIT 768
           L FT   ++ SY +T     R     ++   FG + W D G H VRSPIV+T
Sbjct: 728 LVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759