Cp4.1LG14g03420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG14g03420
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	gibberellin 2-beta-dioxygenase 1-like
Location	Cp4.1LG14: 2348256 .. 2350141 (+)
RNA-Seq Expression	Cp4.1LG14g03420
Synteny	Cp4.1LG14g03420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCCTTCTCTCTCTCTCCTATAAATACCCTTCACCTCTCCCTCCCTCTCTCCACACTCCTTTTGTTCTTCCTCTGTTTCTTTAAAGGAGAAAGGAGAAAAAGGGAAACTTTTAGTTCGTTCATTCTTGTTCTTGTTGTTGTTGTTGTTCTTCTTCTTCTCTCAAAACTTTCCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGATTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATTAGAATCCACCAAATTCTTCTCCCTTCCTCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCTGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCTGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGGTAACTAAAACACCCAAATCCCCATTTTCCCAGAATGCTCTGTTTTTCACTCTGTTTCTTATACTCTGTTTTTTTTCTCTCTCTCTGTTCTCTGTTTCTACGTTTTAGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAAGAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAAGAAACATGATCGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTACAATAAACACGACAACCTCTGTTCTTGATAATCAAAAAAAGATTCAAAATTTGAGGTTTTTCCCATCTGGGTTCTCCAAAAAAACATCACCCACCATGTTCTTCATTAAAATAATGAAACTTTTTGTGTCGATGAAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGAGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAATCTTTATCCAAAATTAGGGTTGGAGATGTTCGGGTAGTAGTTCGGTTCATATGGACGTGTCGAGATTCGAACTTTCGATCCCTAACTGTTAGCTTTTGGATAGTTTGGTTGAACGATAAGTAGAGATGACAGAATTCGAACATCTGATCTCTTAGTTTATCAATCAGTGTTTAGATAGTCGGTGTTTTTTTTGTTAGTTTGTTATCCTTGTACACACAGTCTTCTTAATACAAAGGGTATTACTGTTTTTTCTTTTAGGGTATTATGAGTTGTCAAAAATAGTACAACACGTTGGTGTTTGTAAGAAACGTGGGAGTTCTCGAAAAGAAGGGTTGTTGTCTCGAGATGTCTATTTGTGAGAAACGAGGATGTGTAAGTAATAGCCAATGTGTGTTGTCAAAATTTGTCTGAATTATATTCTAATCTTATATATTCCAAAAAATTTCAA

mRNA sequence

ACCCTTCTCTCTCTCTCCTATAAATACCCTTCACCTCTCCCTCCCTCTCTCCACACTCCTTTTGTTCTTCCTCTGTTTCTTTAAAGGAGAAAGGAGAAAAAGGGAAACTTTTAGTTCGTTCATTCTTGTTCTTGTTGTTGTTGTTGTTCTTCTTCTTCTCTCAAAACTTTCCAGCAATCCAACAGTTGTCTTTTGATTCCTTAATGAGAAGCTCCACGTCCATGGCGGCTGATTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATTAGAATCCACCAAATTCTTCTCCCTTCCTCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCTGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCTGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAAGAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAAGAAACATGATCGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGAGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAATCTTTATCCAAAATTAGGGTTGGAGATGTTCGGGTAGTAGTTCGGTTCATATGGACGTGTCGAGATTCGAACTTTCGATCCCTAACTGTTAGCTTTTGGATAGTTTGGTTGAACGATAAGTAGAGATGACAGAATTCGAACATCTGATCTCTTAGTTTATCAATCAGTGTTTAGATAGTCGGTGTTTTTTTTGTTAGTTTGTTATCCTTGTACACACAGTCTTCTTAATACAAAGGGTATTACTGTTTTTTCTTTTAGGGTATTATGAGTTGTCAAAAATAGTACAACACGTTGGTGTTTGTAAGAAACGTGGGAGTTCTCGAAAAGAAGGGTTGTTGTCTCGAGATGTCTATTTGTGAGAAACGAGGATGTGTAAGTAATAGCCAATGTGTGTTGTCAAAATTTGTCTGAATTATATTCTAATCTTATATATTCCAAAAAATTTCAA

Coding sequence (CDS)

ATGAGAAGCTCCACGTCCATGGCGGCTGATTCTTCTTCTTCCTCTGCAGCATTCTTCTCTGGGATTCCATTGATAGACCTCTCTGCACCAGATGCTAAACAACTCATTGTCAAAGCTTGTGAAGAACTCGGATTCTTTAAGGTTGTCAACCATGGTGTCCCCATGGAATTCATCTCCTCTCTTGAATTAGAATCCACCAAATTCTTCTCCCTTCCTCTCTCTGAAAAACAGAGAGCTGGCCCTCCTTCCCCTTTTGGCTATGGAAACAAACAAATTGGCTGCAATGGCGATGTCGGTTGGGTTGAATATCTCCTCTTGAACACTCATCTCGAATCCAACTCTGATGGGTTCCTCTCCATTTTTGGCCAAGACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTCGGCTGTGAGGAACATGGCGGGTGAAATCCTCGAGCTAATGGCGGAAGGGTTGATGATTCAACAACGAAACGTGTTCAGTAAGCTCGTGATGGATGAAGAGAGCGACTCTGTTTTCAGAGTGAACCATTATCCGCCATGTCCAGACCTTCAAGCTTTAAAAGGAAGAAACATGATCGGATTTGGAGAACACACAGACCCTCAAATCATTTCAGTTTTGAGATCAAACAACACTTCTGGATTTCAAATCTCTCTCGCAGATGGGAATTGGATATCTGTTCCTCCCGATCACAGCTCCTTCTTCATCAATGTTGGCGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGAGTTTTGACAAACAGCTCGAAGTCAAGGGTTTCAATGATCTACTTCGGTGGGCCACCGTTGAGTGAAAAGATAGCTCCTTTAGCTTCCCTTATGCAAGGAGAAGAAAGAAGTTTGTACAAAGAGTTTACATGGTTTGAGTACAAAAGATCAGCTTACAACTCCAGGTTGGCTGACAACAGGCTTGTGCCCTTTGAAAGAATTGCAGCCTCATAA

Protein sequence

MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVPFERIAAS

Homology

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BLAST of Cp4.1LG14g03420 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 9.1e-128
Identity = 225/311 (72.35%), Postives = 260/311 (83.60%), Query Frame = 0


Query: 18  FFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEKQ 77
           F S IPL+DLS PDAK LIVKACE+ GFFKV+NHG+P++ IS LE E+ KFFSLP +EK+
Sbjct: 23  FSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQTEKE 82

Query: 78  RAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIS 137
           +AGP +PFGYGNK+IG NGD+GW+EYLLL T    N D   S++G+D  K R  + DY  
Sbjct: 83  KAGPANPFGYGNKRIGLNGDIGWIEYLLLTT----NQDHNFSLYGEDIDKFRGLLKDYKC 142

Query: 138 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQALKGRNMIGF 197
           A+RNMA EIL+LMAEGL IQ +NVFSKLVMD++SD +FRVNHYP CP+L A+ G N+IGF
Sbjct: 143 AMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNHYPACPEL-AINGENLIGF 202

Query: 198 GEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKH 257
           GEHTDPQIIS+LRSNNTSGFQISL DG+WISVPPDHSSFFINVGDSLQVMTNGRFKSV+H
Sbjct: 203 GEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFINVGDSLQVMTNGRFKSVRH 262

Query: 258 RVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADN 317
           RVL N    R+SMIYF GPPLSEKIAPL SLM+G+E SLYKEFTWFEYK S Y SRLADN
Sbjct: 263 RVLANGIDPRLSMIYFCGPPLSEKIAPLPSLMKGKE-SLYKEFTWFEYKSSTYGSRLADN 322

Query: 318 RLVPFERIAAS 329
           RL  +ERIAA+
Sbjct: 323 RLGNYERIAAT 327

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BLAST of Cp4.1LG14g03420 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 8.2e-121
Identity = 209/313 (66.77%), Postives = 254/313 (81.15%), Query Frame = 0


Query: 15  SAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLS 74
           S   F+GIP++DL+ PDAK LIV AC + GFFK+VNHGVP+E +++LE E+ +FF    S
Sbjct: 20  STPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQS 79

Query: 75  EKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVND 134
           EK RAGPP PFGYG+K+IG NGDVGWVEYLLLNT+ +  S   L IF ++P   R+ V +
Sbjct: 80  EKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVEN 139

Query: 135 YISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQALKGRNM 194
           YI+AV+NM   +LELMAEGL I+QRN  S+L+ DE+SDS FR+NHYPPCP++QAL  RN+
Sbjct: 140 YITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL-NRNL 199

Query: 195 IGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKS 254
           +GFGEHTDPQIISVLRSN+TSG QI L DG W+SVPPD +SFFINVGD+LQVMTNGRFKS
Sbjct: 200 VGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNGRFKS 259

Query: 255 VKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRL 314
           VKHRVL +++KSR+SMIYFGGP LSE IAPL S+M   E  LYKEFTW EYK++AY SRL
Sbjct: 260 VKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAAYTSRL 319

Query: 315 ADNRLVPFERIAA 328
           ADNRL PF++ AA
Sbjct: 320 ADNRLAPFQKSAA 331

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BLAST of Cp4.1LG14g03420 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 389.0 bits (998), Expect = 5.2e-107
Identity = 192/309 (62.14%), Postives = 235/309 (76.05%), Query Frame = 0


Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEK-Q 78
           FS IP+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 79  RAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIS 138
            AG   PFGYGN +IG NGDVGWVEYLL+N + +S S        + P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 139 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCP--DLQALKGRNMI 198
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G+N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 199 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 258
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 259 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 318
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 319 DNRLVPFER 325
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

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BLAST of Cp4.1LG14g03420 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-102
Identity = 179/303 (59.08%), Postives = 232/303 (76.57%), Query Frame = 0


Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEKQRAGP 81
           IP+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 82  PSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRN 141
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQALKGRNMIGFGEHT 201
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP     +  +    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAE--EEAEKMVKVGFGEHT 210

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 322 FER 325
           FE+
Sbjct: 331 FEK 331

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BLAST of Cp4.1LG14g03420 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.1e-100
Identity = 181/306 (59.15%), Postives = 224/306 (73.20%), Query Frame = 0


Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEKQR 78
           FSG+P++DL +P A + +V ACE  GFFKVVNHGV  + +   E E+ +FFS    +K R
Sbjct: 25  FSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESEAVRFFSQTQPDKDR 84

Query: 79  AGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISA 138
           +GP  PFGYG+K+IG NGD+GW+EYLLL     S +D   +         R+A+N+YIS 
Sbjct: 85  SGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLAD---ACTVPSCAVFRAALNEYISG 144

Query: 139 VRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQALKGRNMIGFG 198
           VR +A  ++E M+EGL I Q +  S LV  E SD VFRVNHYPPC  LQ L G ++ GFG
Sbjct: 145 VRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFRVNHYPPCRALQGL-GCSVTGFG 204

Query: 199 EHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHR 258
           EHTDPQ++SVLRSN TSG QI+L DG W+SVP D  SFF+NVGDSLQV+TNGRFKSVKHR
Sbjct: 205 EHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSLQVLTNGRFKSVKHR 264

Query: 259 VLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNR 318
           V+ NS KSRVS IYFGGPPL+++IAPL  L+   E+SLYKEFTW EYK++AY SRL DNR
Sbjct: 265 VVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQSLYKEFTWDEYKKAAYKSRLGDNR 324

Query: 319 LVPFER 325
           L  FE+
Sbjct: 325 LAQFEK 326

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BLAST of Cp4.1LG14g03420 vs. NCBI nr
Match: XP_023551922.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650 bits (1676), Expect = 4.13e-235
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS
Sbjct: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60

Query: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120

Query: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180
           FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY
Sbjct: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180

Query: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328

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BLAST of Cp4.1LG14g03420 vs. NCBI nr
Match: KAG6577055.1 (Gibberellin 2-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015066.1 Gibberellin 2-beta-dioxygenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 642 bits (1655), Expect = 6.57e-232
Identity = 324/328 (98.78%), Postives = 325/328 (99.09%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS
Sbjct: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60

Query: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LE ESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120

Query: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180
           FGQDPQKLRSAVNDYI AVRNMAGEILELMAEGLMIQQRNVFSKLVMDE+SDSVFRVNHY
Sbjct: 121 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 180

Query: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328

[Close]

BLAST of Cp4.1LG14g03420 vs. NCBI nr
Match: XP_022931327.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 634 bits (1635), Expect = 1.85e-227
Identity = 322/328 (98.17%), Postives = 323/328 (98.48%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAA SSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS
Sbjct: 88  MRSSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 147

Query: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LE ESTKFFSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 148 LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 207

Query: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180
           FGQDPQKLRSAVNDYI AVRNMAGEILELMAEGLMIQQRNVFSKLVMDE+SDSVFRVNHY
Sbjct: 208 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 267

Query: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 268 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 327

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 328 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 387

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 388 TWFEYKRSAYNSRLADNRLVPFERIAAS 415

[Close]

BLAST of Cp4.1LG14g03420 vs. NCBI nr
Match: XP_022985064.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 621 bits (1601), Expect = 1.08e-223
Identity = 317/328 (96.65%), Postives = 320/328 (97.56%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAA +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISS
Sbjct: 1   MRSSTSMAA-ASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISS 60

Query: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LE ESTKFFSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 120

Query: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180
           FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGL IQQRNVFSKLVMDE+SDSVFRVNHY
Sbjct: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHY 180

Query: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 327

[Close]

BLAST of Cp4.1LG14g03420 vs. NCBI nr
Match: CAC85924.1 (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 613 bits (1580), Expect = 1.37e-220
Identity = 309/320 (96.56%), Postives = 312/320 (97.50%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKF 68
           A +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLE ESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 121

Query: 129 RSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQA 188
           RSAVNDYISAVRNMAGEILELMAEGL IQQRNVFSKLVMDE+SDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 328
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

[Close]

BLAST of Cp4.1LG14g03420 vs. ExPASy TrEMBL
Match: A0A6J1ETB7 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111437550 PE=3 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 8.94e-228
Identity = 322/328 (98.17%), Postives = 323/328 (98.48%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAA SSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS
Sbjct: 88  MRSSTSMAAASSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 147

Query: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LE ESTKFFSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 148 LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 207

Query: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180
           FGQDPQKLRSAVNDYI AVRNMAGEILELMAEGLMIQQRNVFSKLVMDE+SDSVFRVNHY
Sbjct: 208 FGQDPQKLRSAVNDYILAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEQSDSVFRVNHY 267

Query: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 268 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 327

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 328 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 387

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 388 TWFEYKRSAYNSRLADNRLVPFERIAAS 415

[Close]

BLAST of Cp4.1LG14g03420 vs. ExPASy TrEMBL
Match: A0A6J1JC86 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483149 PE=3 SV=1)

HSP 1 Score: 621 bits (1601), Expect = 5.22e-224
Identity = 317/328 (96.65%), Postives = 320/328 (97.56%), Query Frame = 0


Query: 1   MRSSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISS 60
           MRSSTSMAA +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISS
Sbjct: 1   MRSSTSMAA-ASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISS 60

Query: 61  LELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSI 120
           LE ESTKFFSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSI
Sbjct: 61  LESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSI 120

Query: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHY 180
           FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGL IQQRNVFSKLVMDE+SDSVFRVNHY
Sbjct: 121 FGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHY 180

Query: 181 PPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240
           PPCPDLQALKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV
Sbjct: 181 PPCPDLQALKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINV 240

Query: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300
           GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF
Sbjct: 241 GDSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEF 300

Query: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 328
           TWFEYKRSAYNSRLADNRLVPFERIAAS
Sbjct: 301 TWFEYKRSAYNSRLADNRLVPFERIAAS 327

[Close]

BLAST of Cp4.1LG14g03420 vs. ExPASy TrEMBL
Match: Q7X9N5 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1)

HSP 1 Score: 613 bits (1580), Expect = 6.62e-221
Identity = 309/320 (96.56%), Postives = 312/320 (97.50%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKF 68
           A +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLE ESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 121

Query: 129 RSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQA 188
           RSAVNDYISAVRNMAGEILELMAEGL IQQRNVFSKLVMDE+SDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 328
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

[Close]

BLAST of Cp4.1LG14g03420 vs. ExPASy TrEMBL
Match: Q8GUE8 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 1.90e-220
Identity = 308/320 (96.25%), Postives = 312/320 (97.50%), Query Frame = 0


Query: 9   ADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKF 68
           A +SS SAAF+SGIPLIDLSAPDAKQLIVKACEELGFFKVV HGVPME ISSLE ESTKF
Sbjct: 2   AAASSFSAAFYSGIPLIDLSAPDAKQLIVKACEELGFFKVVKHGVPMELISSLESESTKF 61

Query: 69  FSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 128
           FSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLS+FGQDPQKL
Sbjct: 62  FSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSMFGQDPQKL 121

Query: 129 RSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQA 188
           RSAVNDYISAVRNMAGEILELMAEGL IQQRNVFSKLVMDE+SDSVFRVNHYPPCPDLQA
Sbjct: 122 RSAVNDYISAVRNMAGEILELMAEGLKIQQRNVFSKLVMDEQSDSVFRVNHYPPCPDLQA 181

Query: 189 LKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 248
           LKG NMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT
Sbjct: 182 LKGTNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMT 241

Query: 249 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 308
           NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS
Sbjct: 242 NGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRS 301

Query: 309 AYNSRLADNRLVPFERIAAS 328
           AYNSRLADNRLVPFERIAAS
Sbjct: 302 AYNSRLADNRLVPFERIAAS 321

[Close]

BLAST of Cp4.1LG14g03420 vs. ExPASy TrEMBL
Match: A0A6J1FNB4 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447347 PE=3 SV=1)

HSP 1 Score: 585 bits (1508), Expect = 1.25e-209
Identity = 300/323 (92.88%), Postives = 306/323 (94.74%), Query Frame = 0


Query: 6   SMAADSSSSSAAFFSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELES 65
           SMAA + SSSAAFFSG+PLIDLSAPDAK LIVKACEELGFFKVVNHGVPMEFIS+LE ES
Sbjct: 20  SMAA-AGSSSAAFFSGVPLIDLSAPDAKHLIVKACEELGFFKVVNHGVPMEFISTLESES 79

Query: 66  TKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDP 125
           TKFFSLPLSEKQRAGPPSPFGYGNKQIG NGDVGWVEYLLLNTHLESNSDGFLSIFGQDP
Sbjct: 80  TKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEYLLLNTHLESNSDGFLSIFGQDP 139

Query: 126 QKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPD 185
           QKLRSAVNDYISAVRNMA EILELMAEGL IQQRN FSKL+MDEESDSVFRVNHYPPCP+
Sbjct: 140 QKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFSKLLMDEESDSVFRVNHYPPCPE 199

Query: 186 LQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQ 245
           LQ LKG NMIGFGEHTDPQIISVLRSNNTSG QISLADGNWISVPPD SSFFINVGDSLQ
Sbjct: 200 LQTLKGTNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQSSFFINVGDSLQ 259

Query: 246 VMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEY 305
           VMTNGRFKSVKHRVLTN  KSRVSMIYFGGPPLSEKIAPL SLM+GEE SLYKEFTWFEY
Sbjct: 260 VMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEY 319

Query: 306 KRSAYNSRLADNRLVPFERIAAS 328
           KRSAYNSRLADNRLV FERIAAS
Sbjct: 320 KRSAYNSRLADNRLVHFERIAAS 340

[Close]

BLAST of Cp4.1LG14g03420 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 389.0 bits (998), Expect = 3.7e-108
Identity = 192/309 (62.14%), Postives = 235/309 (76.05%), Query Frame = 0


Query: 19  FSGIPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEK-Q 78
           FS IP+ID+S P++K  +VKACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 79  RAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYIS 138
            AG   PFGYGN +IG NGDVGWVEYLL+N + +S S        + P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 139 AVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCP--DLQALKGRNMI 198
           +VR M  ++LE + +GL I+ RN  SKLV D+ +DS+ R+NHYPPCP  + +   G+N+I
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVI 194

Query: 199 GFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSV 258
           GFGEHTDPQIISVLRSNNTSG QI+L DG+WISVPPDH+SFF NVGDSLQVMTNGRFKSV
Sbjct: 195 GFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSV 254

Query: 259 KHRVLTNSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLA 318
           +HRVL N  KSRVSMIYF GP L+++IAPL  L+  E+  LY+EFTW EYK S YNSRL+
Sbjct: 255 RHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLS 314

Query: 319 DNRLVPFER 325
           DNRL  FER
Sbjct: 315 DNRLQQFER 321

[Close]

BLAST of Cp4.1LG14g03420 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 373.2 bits (957), Expect = 2.1e-103
Identity = 179/303 (59.08%), Postives = 232/303 (76.57%), Query Frame = 0


Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEKQRAGP 81
           IP+++L+ P+AK  IVKACEE GFFKVVNHGV  E ++ LE E+  FF LP S K RAGP
Sbjct: 31  IPVVNLADPEAKTRIVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGP 90

Query: 82  PSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRN 141
           P P+GYGNK+IG NGDVGW+EYLLLN + + +S    ++F Q PQ  R +V +Y+  ++ 
Sbjct: 91  PEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKE 150

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQALKGRNMIGFGEHT 201
           ++ ++LE++AE L I+ R+  SK++ DE+SDS  R+NHYP     +  +    +GFGEHT
Sbjct: 151 VSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAE--EEAEKMVKVGFGEHT 210

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQIISVLRSNNT+G QI + DG+W++VPPDHSSFFINVGD+LQVMTNGRFKSVKHRVL 
Sbjct: 211 DPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLA 270

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           ++ +SR+SMIYFGGPPLS+KIAPL  L+  ++  LYKEFTW +YK SAY S+L D RL  
Sbjct: 271 DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGL 330

Query: 322 FER 325
           FE+
Sbjct: 331 FEK 331

[Close]

BLAST of Cp4.1LG14g03420 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 348.2 bits (892), Expect = 7.2e-96
Identity = 170/303 (56.11%), Postives = 219/303 (72.28%), Query Frame = 0


Query: 22  IPLIDLSAPDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFFSLPLSEKQRAGP 81
           IP+IDL+  DAK  IVKACEE GFFKV+NHGV  + ++ LE E+  FF+L  S K +AGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 82  PSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRN 141
           P PFGYG K+IG NGD+GW+EY+LLN +L   S    +IF   P   R AV +YI  ++ 
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 142 MAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDLQALKGRNMIGFGEHT 201
           M+ + LE++ E L I+ +   S+LV  +ESDS  R+NHY   P+ +    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 202 DPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVGDSLQVMTNGRFKSVKHRVLT 261
           DPQ+IS+LRSN+T G QI + DG W+ V PDHSSFF+ VGD+LQVMTNGRFKSVKHRV+T
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 262 NSSKSRVSMIYFGGPPLSEKIAPLASLMQGEERSLYKEFTWFEYKRSAYNSRLADNRLVP 321
           N+ +SR+SMIYF GPPLSEKIAPL+ L+  ++  LY EFTW +YK SAY ++L D RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 326

Query: 322 FER 325
           FE+
Sbjct: 327 FEK 326

[Close]

BLAST of Cp4.1LG14g03420 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 293.5 bits (750), Expect = 2.1e-79
Identity = 158/327 (48.32%), Postives = 208/327 (63.61%), Query Frame = 0


Query: 3   SSTSMAADSSSSSAAFFSGIPLIDLSAPDAKQL---IVKACEELGFFKVVNHGVPMEFIS 62
           SST +      + ++     P+ID S  D  +L   IVKACE  GFFKV+NHGV  E I 
Sbjct: 5   SSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIK 64

Query: 63  SLELESTKFFSLPLSEKQRAGPPSPFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLS 122
             E E  +FF+ P S+K RAGP SPFGYG K IG NGD+G +EYLLL+ +  + +D   +
Sbjct: 65  RFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSET 124

Query: 123 IFGQDPQKLRSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNH 182
           I   DP K  SA NDYI  VR++A EI++L  E L  Q+ +  S+L+ D  SDS+ R+NH
Sbjct: 125 ISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNH 184

Query: 183 YPPCPDLQALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFIN 242
           YPP P   AL G   IGFGEH+DPQI++VLRSN+  G +I   DG WI +P D + FF+ 
Sbjct: 185 YPPAP--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVL 244

Query: 243 VGDSLQVMTNGRFKSVKHRVLTNSSKS-RVSMIYFGGPPLSEKIAPLASLMQGEERSLYK 302
           VGD LQ +TNGRF SV+HRVL N++K  R+S +YF  PPL  KI+PL  ++  E    Y 
Sbjct: 245 VGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYN 304

Query: 303 EFTWFEYKRSAYNSRLADNRLVPFERI 326
            FTW +YK++ Y+ RL   RL  F+ +
Sbjct: 305 SFTWGDYKKATYSLRLDVPRLEFFKTL 329

[Close]

BLAST of Cp4.1LG14g03420 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 278.1 bits (710), Expect = 9.1e-75
Identity = 161/322 (50.00%), Postives = 195/322 (60.56%), Query Frame = 0


Query: 12  SSSSAAFFSGIPLIDLS--APDAKQLIVKACEELGFFKVVNHGVPMEFISSLELESTKFF 71
           S    A    IP+ID+S         IVKACE LGFFKV+NHGV    IS +E ES  FF
Sbjct: 5   SQKIVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFF 64

Query: 72  SLPLSEKQRAGPPS-PFGYGNKQIGCNGDVGWVEYLLLNTHLESNSDGFLSIFGQDPQKL 131
           + P  EK+   P + PF YG + IG NGD G VEYLL +T    N   F S         
Sbjct: 65  AKPAHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHT----NDPAFRS-----QLSF 124

Query: 132 RSAVNDYISAVRNMAGEILELMAEGLMIQQRNVFSKLVMDEESDSVFRVNHYPPCPDL-- 191
            SAVN YI AV+ +A EIL+L AEGL +   + FS+L+   +SDSV RVNHYPP      
Sbjct: 125 SSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFG 184

Query: 192 -----QALKGRNMIGFGEHTDPQIISVLRSNNTSGFQISLADGNWISVPPDHSSFFINVG 251
                        +GFGEHTDPQI++VLRSN   G Q+S +DG W+SV PD S+F +NVG
Sbjct: 185 EANLSDQSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVG 244

Query: 252 DSLQVMTNGRFKSVKHRVLTNSSKSRVSMIYFGGPPLSEKIAPL-ASLMQGEERSLYKEF 311
           D LQVMTNGRF SV+HR LT   +SR+S  YF GPPL  KI PL A +M   +  LY+ F
Sbjct: 245 DLLQVMTNGRFISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTF 304

Query: 312 TWFEYKRSAYNSRLADNRLVPF 323
           TW EYK+ AY+ RL D+RL  F
Sbjct: 305 TWGEYKKRAYSLRLEDSRLDMF 316

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g03420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SQ80	9.1e-128	72.35	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
Q9XG83	8.2e-121	66.77	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q8LEA2	5.2e-107	62.14	Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ...	[more]
Q9XFR9	2.9e-102	59.08	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q8S0S6	6.1e-100	59.15	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of Cp4.1LG14g03420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023551922.1	4.13e-235	100.00	gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6577055.1	6.57e-232	98.78	Gibberellin 2-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022931327.1	1.85e-227	98.17	gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
XP_022985064.1	1.08e-223	96.65	gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima]	[more]
CAC85924.1	1.37e-220	96.56	Gibberellin 2-oxidase [Cucurbita maxima]	[more]

BLAST of Cp4.1LG14g03420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ETB7	8.94e-228	98.17	gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111437...	[more]
A0A6J1JC86	5.22e-224	96.65	gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148314...	[more]
Q7X9N5	6.62e-221	96.56	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1	[more]
Q8GUE8	1.90e-220	96.25	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox PE=2 SV=1	[more]
A0A6J1FNB4	1.25e-209	92.88	gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]

BLAST of Cp4.1LG14g03420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78440.1	3.7e-108	62.14	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.1	2.1e-103	59.08	gibberellin 2-oxidase	[more]
AT2G34555.1	7.2e-96	56.11	gibberellin 2-oxidase 3	[more]
AT1G02400.1	2.1e-79	48.32	gibberellin 2-oxidase 6	[more]
AT1G47990.1	9.1e-75	50.00	gibberellin 2-oxidase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 36..53 score: 54.55 coord: 200..221 score: 27.69 coord: 236..262 score: 33.0
None	No IPR available	PANTHER	PTHR47990:SF150	GIBBERELLIN 2-BETA-DIOXYGENASE 1	coord: 16..324
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 16..324
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 19..316
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..316 e-value: 2.0E-78 score: 266.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 22..112 e-value: 4.9E-15 score: 56.3
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 172..274 e-value: 6.9E-28 score: 97.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 170..276 score: 12.346098

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g03420.1	Cp4.1LG14g03420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding