Cp4.1LG13g09220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g09220
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionglycosylphosphatidylinositol anchor attachment 1 protein-like
LocationCp4.1LG13: 7626568 .. 7630456 (+)
RNA-Seq ExpressionCp4.1LG13g09220
SyntenyCp4.1LG13g09220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACCGACCGGGCCGAATCAACGGGTTCATATTGGTTTATTATAATGCCGAACCGACCGACGAGACCGCATGTTGGCCGGTTCATATGATGGTTAACTGAGCTCACGTTCAGTTCTTCTACCCTTTTCTTCTTCTTCTTCTTCTTCTTGGTTTCCATTTTTGCAACAGATTCAGAGCAATTGGGCGACTGTGAAGAGCACCTCTTCCGATGGCTGAAACCGAGAAGCCAAAATCGAAGGCGCGGCCGATTGTGCGCCTGGGGATTTTCCTAATTTCTCACAGTATTTTCTTCAGGTGCGCTGTTGTTGATTTTACATGGATTTGATTTTCAGTTCGTATCAGAATCTAGTGATTGATTAATGTCCTTGTGATTTTCTTTTATTTTTTTGGGTGTTTTCTAGCGTGATTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTGCTACTACCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTCCCTTTGATATTCATCATTTTTTGTGTTCTCGTGATTGATCGTCAGTTGAGTTGAATGATTTAAGATATATTACGAATTAGGATTCATTTAATGCTGCGAAGTATCTAAAATTATAGGATCATCAATTTATGCCTTCTTGTTCTTTTGCACTGATCTATTCATTTTACCTTTATTTGGATAGAACAGGTCTCTTAATTGTGGCACTATATTTTCCCACAGGTTCTGCAAATACCATGCTCTCGGGTCAGGAAGTCTCAGAGGCGAACGACTTGGTGAAGGATATGAAGGGTTTGAATTCGAAGTCTGGCTCAACAATCTTGTAAGTATTTATGCACTAGTTTTATCCCTTTTACTTCTCCTTTAATACCTTAATGGAAGCTTTTTTATGCTTAAGATTTTGAAAATCACCACTTCTTAGTGTAAATTTTTTCTACCTGAAGATTATGATAAACAAATTTGATTTTCTATACATTTTTCAGTGGAAGCCAGCAGATTTTAGCACAATATATATCAAACTTGGGTGCAGAAGTTAATTACCACAGGTTCCAACCGCAGTCTAGTCAGTTCCATCCACTGCACTTTTTTTCTAGCCCTGATTCTGGGATCGTTCAGGACAATGTTAGTTGCATGTCACAGGGTATTAACACTGTTGGGATAATAAGAGCACCACAAGCAGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAAGACTAGTCTTCATGAAACTTTGTCTCTTGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTCACTTGGTTGGCTAAAGACATTGTATGGCTTGCTGCGGATTCACGATATGGGGAATATGCTGCAGTTTCAGCTTGGCTTAGAGATTATCACACTCCTTCATTTGGTCGATCCAGCATGATTGACACATATACTTGTGCTGAAACAAATGTCCTTTATGAATTTGAAGCAAACCATGTAACAGAGAAAGAGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTCATTAAGGTTTCAAATAGAAGTGAACACTTTGAGGATAGTCTTAGTGTTTATGCTGAAGCGTCTAATGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTATCTAGCAGAACACAGACAAGGTTTTCGGATTAAGATCGAGAAATTTTGGCCTTTACTTGACTGCAAATGGCTCAAGTTTTTGGGCGAATTTTTTGAGTCAATAGGAAAGTTTATCCGAAGCTTGAACCCTGAATGGAAGTTTGGCATGCTAGCTTCTGACTATGTTGATGGCACCGCCACACTTGCAAGTTCATTGTACTACCAGGTGGTTTGCACTTCTGTTCATATTCTTGGAGATTGTTGTTTATAGTTTCATTTAATGAAATAAGGAAAGATTTTTCATTAATGTGAACAATGGAGATTATTTCACTTAAATTTTCTATGTTCTTCTTGCCATTAGGCTGTGGGTATTCCTACTGGCCCTCATGGAGCTTTTCGAGATTACCAAATTGATGCTATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCCGGTCAGTGCTCATTTTGCCTCAATGATTTTATAATGTGCTCTCCTCCCACCCCTACTTTTCAGGGCTCACTACATGAATAAATGTATGGTTTCCTTAATGGGGTTCGGTCTACATTAAACAATATTTTCTACTCTTATTTTAATTATACATCATTTTTCATTTTTATCCTAAGTGTTTTACTGTCGATCTTCGATGTTTAAATTTTAATTTGTAACTAGTTTTAATGGGGGCATGAGTAAATACTTTTTCTTCCCAAAAATAAATTTTGATTTGACTGATAGTTTCTCTTTTTAAATGACTAGAATTTACGTGTTAAATTCATTCAAGTGAATTTATTGCCTAAAGTCATGCTGATGTATGGAAATGAGTTTAGATTTCGACGCCTTGAATGATCAAGCTATTCATAATTGGATTTGAAATTAGAAATTGACAACTTAGCGCCCAGCTTGCTGGCAATTCTGCATGCTAGCAATGAAATTCTTCTGATCCTTTTGATTTGGCTAAGGATTTTGTATTTGGTTGTGCAGGCTAATTGAAGGAGTTGTACGGTCAGTGAATAACCTCTTGGAGAAGTTTCATCAATCATTCTTTCTATACCTTATGGTATCTACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCACTCCTAGTTGCTCCACTACCAGCAGTTGCGGCCTCTCTTTATTCTTATGCTAATAACTTGAATCTAACCTTGGAAAAAATCGAGCCTGCAGCTCCAGCAAATCCTGATGATGTGCTTATTGTCTCTTTAAGATCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGTGCTGTTGTTTCATTACTTCCATACTTCATCTGCCAAATACCTGGTTACAGTCCCACAACAAACTCTATTATTTGGGCGTTGCTTTCACTGCTCACGCTACTAATTCTGTCGGTGATATTGGGCTCTCCACTTAGTTCAACTAAATCTTGTGAACAGCGGATTCAAGAGTGGGCGTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATCGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCCTTCTTGGTGGTGTCCATGTGCTTGCTGGCGCATCCCCTGAAGCTTGATTTAGTGGCAGGGAACTTTAAAGCTCTCTCAAGGGCAGCCAGTAACCTCGTTTTGGGATTCATAGCTTTTCCGCCTGTTACTTTCTTCTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATCTACATCTCGGGGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCTTGCTGGGTATTATGTACACAAATTTTACTCCATCCATGTTGAGCTACCTTGTTTAGTTTTTTCCATGACATTTGTTTAGACTTTCACTTAGTCCTGAGAAATTGGTTTCAATGGTATTTGAACTAGTTACACGTTCATCCCTTGCATGGTAGCTTCTGTAAGCAGGCCTTGAAGCCTACTACTTTGTGTCACAGTTGTTTATACTTGTAGGACTGATTCAGTAAGCTTTTACCCTCTTGGAAATTGTTTTTGGCTATTTCTCTTTTCGAACTAATTGGACAATCAATCACCTTCTCTATATCCAATGATCAGTGTCTCTTGAGTTTCTCTCTCCAGTTACTGCATTTAAAGATTATAACACATAGGTAAATGTAGAATTTAGCAACAAATTAGCTGCTGAGGTTTCTTTAAATCCTTGTGAGTTGTG

mRNA sequence

GAACCGACCGGGCCGAATCAACGGGTTCATATTGGTTTATTATAATGCCGAACCGACCGACGAGACCGCATGTTGGCCGGTTCATATGATGGTTAACTGAGCTCACGTTCAGTTCTTCTACCCTTTTCTTCTTCTTCTTCTTCTTCTTGGTTTCCATTTTTGCAACAGATTCAGAGCAATTGGGCGACTGTGAAGAGCACCTCTTCCGATGGCTGAAACCGAGAAGCCAAAATCGAAGGCGCGGCCGATTGTGCGCCTGGGGATTTTCCTAATTTCTCACAGTATTTTCTTCAGCGTGATTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTGCTACTACCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTCTCGGGTCAGGAAGTCTCAGAGGCGAACGACTTGGTGAAGGATATGAAGGGTTTGAATTCGAAGTCTGGCTCAACAATCTTTGGAAGCCAGCAGATTTTAGCACAATATATATCAAACTTGGGTGCAGAAGTTAATTACCACAGGTTCCAACCGCAGTCTAGTCAGTTCCATCCACTGCACTTTTTTTCTAGCCCTGATTCTGGGATCGTTCAGGACAATGTTAGTTGCATGTCACAGGGTATTAACACTGTTGGGATAATAAGAGCACCACAAGCAGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAAGACTAGTCTTCATGAAACTTTGTCTCTTGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTCACTTGGTTGGCTAAAGACATTGTATGGCTTGCTGCGGATTCACGATATGGGGAATATGCTGCAGTTTCAGCTTGGCTTAGAGATTATCACACTCCTTCATTTGGTCGATCCAGCATGATTGACACATATACTTGTGCTGAAACAAATGTCCTTTATGAATTTGAAGCAAACCATGTAACAGAGAAAGAGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTCATTAAGGTTTCAAATAGAAGTGAACACTTTGAGGATAGTCTTAGTGTTTATGCTGAAGCGTCTAATGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTATCTAGCAGAACACAGACAAGGTTTTCGGATTAAGATCGAGAAATTTTGGCCTTTACTTGACTGCAAATGGCTCAAGTTTTTGGGCGAATTTTTTGAGTCAATAGGAAAGTTTATCCGAAGCTTGAACCCTGAATGGAAGTTTGGCATGCTAGCTTCTGACTATGTTGATGGCACCGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGCCCTCATGGAGCTTTTCGAGATTACCAAATTGATGCTATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCCGGCTAATTGAAGGAGTTGTACGGTCAGTGAATAACCTCTTGGAGAAGTTTCATCAATCATTCTTTCTATACCTTATGGTATCTACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCACTCCTAGTTGCTCCACTACCAGCAGTTGCGGCCTCTCTTTATTCTTATGCTAATAACTTGAATCTAACCTTGGAAAAAATCGAGCCTGCAGCTCCAGCAAATCCTGATGATGTGCTTATTGTCTCTTTAAGATCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGTGCTGTTGTTTCATTACTTCCATACTTCATCTGCCAAATACCTGGTTACAGTCCCACAACAAACTCTATTATTTGGGCGTTGCTTTCACTGCTCACGCTACTAATTCTGTCGGTGATATTGGGCTCTCCACTTAGTTCAACTAAATCTTGTGAACAGCGGATTCAAGAGTGGGCGTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATCGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCCTTCTTGGTGGTGTCCATGTGCTTGCTGGCGCATCCCCTGAAGCTTGATTTAGTGGCAGGGAACTTTAAAGCTCTCTCAAGGGCAGCCAGTAACCTCGTTTTGGGATTCATAGCTTTTCCGCCTGTTACTTTCTTCTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATCTACATCTCGGGGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCTTGCTGGGTATTATGTACACAAATTTTACTCCATCCATGTTGAGCTACCTTGTTTAGTTTTTTCCATGACATTTGTTTAGACTTTCACTTAGTCCTGAGAAATTGGTTTCAATGGTATTTGAACTAGTTACACGTTCATCCCTTGCATGGTAGCTTCTGTAAGCAGGCCTTGAAGCCTACTACTTTGTGTCACAGTTGTTTATACTTGTAGGACTGATTCAGTAAGCTTTTACCCTCTTGGAAATTGTTTTTGGCTATTTCTCTTTTCGAACTAATTGGACAATCAATCACCTTCTCTATATCCAATGATCAGTGTCTCTTGAGTTTCTCTCTCCAGTTACTGCATTTAAAGATTATAACACATAGGTAAATGTAGAATTTAGCAACAAATTAGCTGCTGAGGTTTCTTTAAATCCTTGTGAGTTGTG

Coding sequence (CDS)

ATGGCTGAAACCGAGAAGCCAAAATCGAAGGCGCGGCCGATTGTGCGCCTGGGGATTTTCCTAATTTCTCACAGTATTTTCTTCAGCGTGATTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTGCTACTACCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTCTCGGGTCAGGAAGTCTCAGAGGCGAACGACTTGGTGAAGGATATGAAGGGTTTGAATTCGAAGTCTGGCTCAACAATCTTTGGAAGCCAGCAGATTTTAGCACAATATATATCAAACTTGGGTGCAGAAGTTAATTACCACAGGTTCCAACCGCAGTCTAGTCAGTTCCATCCACTGCACTTTTTTTCTAGCCCTGATTCTGGGATCGTTCAGGACAATGTTAGTTGCATGTCACAGGGTATTAACACTGTTGGGATAATAAGAGCACCACAAGCAGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAAGACTAGTCTTCATGAAACTTTGTCTCTTGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTCACTTGGTTGGCTAAAGACATTGTATGGCTTGCTGCGGATTCACGATATGGGGAATATGCTGCAGTTTCAGCTTGGCTTAGAGATTATCACACTCCTTCATTTGGTCGATCCAGCATGATTGACACATATACTTGTGCTGAAACAAATGTCCTTTATGAATTTGAAGCAAACCATGTAACAGAGAAAGAGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTCATTAAGGTTTCAAATAGAAGTGAACACTTTGAGGATAGTCTTAGTGTTTATGCTGAAGCGTCTAATGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTATCTAGCAGAACACAGACAAGGTTTTCGGATTAAGATCGAGAAATTTTGGCCTTTACTTGACTGCAAATGGCTCAAGTTTTTGGGCGAATTTTTTGAGTCAATAGGAAAGTTTATCCGAAGCTTGAACCCTGAATGGAAGTTTGGCATGCTAGCTTCTGACTATGTTGATGGCACCGCCACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGCCCTCATGGAGCTTTTCGAGATTACCAAATTGATGCTATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCCGGCTAATTGAAGGAGTTGTACGGTCAGTGAATAACCTCTTGGAGAAGTTTCATCAATCATTCTTTCTATACCTTATGGTATCTACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCACTCCTAGTTGCTCCACTACCAGCAGTTGCGGCCTCTCTTTATTCTTATGCTAATAACTTGAATCTAACCTTGGAAAAAATCGAGCCTGCAGCTCCAGCAAATCCTGATGATGTGCTTATTGTCTCTTTAAGATCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGTGCTGTTGTTTCATTACTTCCATACTTCATCTGCCAAATACCTGGTTACAGTCCCACAACAAACTCTATTATTTGGGCGTTGCTTTCACTGCTCACGCTACTAATTCTGTCGGTGATATTGGGCTCTCCACTTAGTTCAACTAAATCTTGTGAACAGCGGATTCAAGAGTGGGCGTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATCGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCCTTCTTGGTGGTGTCCATGTGCTTGCTGGCGCATCCCCTGAAGCTTGATTTAGTGGCAGGGAACTTTAAAGCTCTCTCAAGGGCAGCCAGTAACCTCGTTTTGGGATTCATAGCTTTTCCGCCTGTTACTTTCTTCTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATCTACATCTCGGGGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCTTGCTGGGTATTATGTACACAAATTTTACTCCATCCATGTTGA

Protein sequence

MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Homology
BLAST of Cp4.1LG13g09220 vs. ExPASy Swiss-Prot
Match: O43292 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606 GN=GPAA1 PE=1 SV=3)

HSP 1 Score: 114.4 bits (285), Expect = 5.3e-24
Identity = 172/720 (23.89%), Postives = 286/720 (39.72%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           +++  + ++ AR ++RL   L   S    +  F A     L+ P     TY+SENA+   
Sbjct: 4   LSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLA-----LVFPPLTQRTYMSENAMGST 63

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
                 +G +   A    +D      KSG+        L + + ++G EV    F  +  
Sbjct: 64  MVEEQFAGGD--RARAFARDFAAHRKKSGAL---PVAWLERTMRSVGLEVYTQSFSRK-- 123

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
                     PD    + +   M  G N  GI+RAP+A   E++VL  P     T+   +
Sbjct: 124 -------LPFPD----ETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTN---S 183

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
            ++G+  ++ +      + AKDIV+L  +    +     AWL  YH              
Sbjct: 184 QAVGLLLALAAHFRGQIYWAKDIVFLVTEH---DLLGTEAWLEAYHDV------------ 243

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
                        +VT  +S     RAG + AA+ +++S+       SL V  E  NGQ+
Sbjct: 244 -------------NVTGMQSSPLQGRAGAIQAAVALELSS---DVVTSLDVAVEGLNGQL 303

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPE-WKFG 360
           PNLDL+N+     + + G    ++                     GK    L PE W   
Sbjct: 304 PNLDLLNLFQTFCQ-KGGLLCTLQ---------------------GK----LQPEDW--- 363

Query: 361 MLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEF- 420
                 + G  TL   +  QA G P G HG F  Y+++A+T+     F      R+ ++ 
Sbjct: 364 TSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSF------RQYKYD 423

Query: 421 ILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAAS 480
           ++  G+ +EG+ R +N+LLE+ HQSFFLYL+   S+FVS+G+YM A   L+  L   A  
Sbjct: 424 LVAVGKALEGMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALE 483

Query: 481 LYSYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFI 540
           L+   +   + LE  EP     P  V +   +     +    + +    G  + +LP   
Sbjct: 484 LWMQLHEAGMGLE--EPGGAPGP-SVPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLG 543

Query: 541 CQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFI 600
             +             +L+LL +    + L        S +   + W  LK +      +
Sbjct: 544 QHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLAL 603

Query: 601 GLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVT 660
            L  +++ NF+   L A  +V    LA P     +      L+  A+ L LG       +
Sbjct: 604 QLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATL-LG-------S 618

Query: 661 FFLFKAALQGFDNLHLGDFWNWMETLWA--------WNSATFLYLGMVHLPCWVLCTQIL 711
            FL++   +    L L + W       A        + +  F  L +   PCW+L   +L
Sbjct: 664 LFLWRELQEA--PLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVL 618

BLAST of Cp4.1LG13g09220 vs. ExPASy Swiss-Prot
Match: Q9WTK3 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=10090 GN=Gpaa1 PE=1 SV=3)

HSP 1 Score: 112.5 bits (280), Expect = 2.0e-23
Identity = 180/732 (24.59%), Postives = 300/732 (40.98%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLL--PMFAMNTYISENALM 60
           +++  + ++ AR ++RL   L        V+ + AG+   L L  P     TY+SENA+ 
Sbjct: 4   LSDPVRRRALARIVLRLNTPL-------CVLSYVAGIAWFLALAFPPLTQRTYMSENAM- 63

Query: 61  PGSANTMLSGQEV--SEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQ 120
               +TM+  Q V    A    +D      K G+        L + + ++G EV    F 
Sbjct: 64  ---GSTMVEEQFVGGDRARSFARDFAAHRKKPGAL---PVAWLERSMRSVGLEVYTQSFS 123

Query: 121 PQSSQFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTS 180
            +            PD    + +   M  G N  GI+RAP++   E++VL  P  P  T+
Sbjct: 124 RK---------LPFPD----ETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATN 183

Query: 181 LHETLSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMI 240
              + ++G+  ++ +      + AKDI++L  D    +     AWL  YH          
Sbjct: 184 ---SQAVGLLLALAAHFRGQIYWAKDIIFLVTDH---DLLGTEAWLEAYHD--------- 243

Query: 241 DTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEAS 300
                            +VT  +S     RAG + AA+ +++S+       SL V  E  
Sbjct: 244 ----------------INVTGIQSSPLQGRAGAIQAAVALELSS---DVVTSLDVTVEGL 303

Query: 301 NGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEW 360
           NGQ+PNLDL+N+     + + G    ++    L    W    G                 
Sbjct: 304 NGQLPNLDLLNLFQTFCQ-KGGLLCTLQ--GKLQPQDWTSLEGP---------------- 363

Query: 361 KFGMLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRD 420
                    + G  TL   +  QA G P GPHG F  Y ++A+T+     F      R+ 
Sbjct: 364 ---------LQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSF------RQY 423

Query: 421 EFILRG-GRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAV 480
           ++ L   G+ +EG+ R +N+LLE+ HQSFF YL+ + S+FVS+G+YM A   L+  L   
Sbjct: 424 KYDLATVGKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLK 483

Query: 481 AASLYSYANNLNLTLEKI----EPAAPANP-DDVLIVSLRSWKWLNAAKRVFVVHLWGAV 540
           A  L+   +   +  E+      P  P  P   V + SL +   ++ A            
Sbjct: 484 ALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQGVGLASLTAPLLISQA------------ 543

Query: 541 VSLLPYFICQIPGYSPTTN-SIIWALLSLLTLLILSVI-LGSPLSSTKSCEQRIQE--WA 600
           + L  YF+  +  +  T +  +  A   +LTLL + V  L  P ++ +    ++ +  W 
Sbjct: 544 MGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRGWM 603

Query: 601 FLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASN 660
            LK +      + L  ++++NF+   L A  +V    LA P     +   + AL    S 
Sbjct: 604 ALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGPRTL---YAALLVVTSP 618

Query: 661 LVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETL--------WAWNSATFLYLGMV 711
            V  F      + FL++  L+    L L + W    T         + + +  F  L + 
Sbjct: 664 AVTLF-----GSLFLWRELLE--VPLSLAEGWQLFLTALAQGVLEHYTYGALLFPILALG 618

BLAST of Cp4.1LG13g09220 vs. ExPASy Swiss-Prot
Match: P39012 (GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAA1 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.7e-17
Identity = 123/485 (25.36%), Postives = 204/485 (42.06%), Query Frame = 0

Query: 12  RPIVRLGIF--LISHSIFFSVICFSAGVLALLLLPMFAM--NTYISENALMPGSANTMLS 71
           R IV +G+   +I+     S++C   G +++ +LPM      TYISENALMP  A +   
Sbjct: 9   RRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYSYFR 68

Query: 72  GQE---VSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHP 131
             E   +      +K+M  + S   + + GS      ++   G +   +    ++ Q+  
Sbjct: 69  ESEWNILRGYRSQIKEMVNMTSMERNNLMGS------WLQEFGTKTAIY----ENEQY-- 128

Query: 132 LHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTP-YNPVKTSLHETLSL 191
                                G    G++ AP+ DG EA+VL  P +N          +L
Sbjct: 129 ---------------------GETLYGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAAL 188

Query: 192 GIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAE 251
           G++ + F     V W    IV  + + R    AA+ +W+  YHT                
Sbjct: 189 GVSLARFFSRWPV-WSKNIIVVFSENPR----AALRSWVEAYHT---------------- 248

Query: 252 TNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNL 311
                           SLD     G++ AA+V+  S+  + FE  + +  +  NG++PNL
Sbjct: 249 ----------------SLD--LTGGSIEAAVVLDYSSTEDFFE-YVEISYDGLNGELPNL 308

Query: 312 DLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLAS 371
           DL+NI   + EH +G ++                             SL+      +  +
Sbjct: 309 DLVNIAISITEH-EGMKV-----------------------------SLHGLPSDQLTNN 368

Query: 372 DYVDGTATLASSLYYQAVGIPTGPHG--AFRDYQIDAITVEMSPKFSSGVKVRRDEFILR 431
           ++      L   +   A+     PHG  AF  ++I ++T++     +SG        I  
Sbjct: 369 NFWSRLKILCLGIRDWALSGVKKPHGNEAFSGWRIQSVTLKAHG--NSG------HDITT 379

Query: 432 GGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYM-IAFALLVAPLPAVAASLY 486
            GR+ E + RS+NNLLEKFHQSFF YL+++  +FVS+  Y+  A AL +A      +SL 
Sbjct: 429 FGRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIA---FAISSLN 379

BLAST of Cp4.1LG13g09220 vs. ExPASy Swiss-Prot
Match: Q9US48 (GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gaa1 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 4.8e-17
Identity = 155/689 (22.50%), Postives = 264/689 (38.32%), Query Frame = 0

Query: 15  VRLGIFLISHSIFFSVICFSAGVLALLLLPMFAM--NTYISENALMPGSANTMLS---GQ 74
           +R+  FL  H  F  +     G+  + +LP   +    ++SE+AL+PG  NT       +
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSK 68

Query: 75  EVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFS 134
            VS +         L++  G+        L Q  + +G         P   Q + ++   
Sbjct: 69  TVSSSLTAANTWSHLDASVGTNTMYDD--LEQIFTAMGL--------PTQKQNYSINIPG 128

Query: 135 SPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSI 194
           S               G N +  +RAP+ D  E+++L  P+   K  + +    G+A +I
Sbjct: 129 SE------------FNGSNFITTLRAPRGDATESLLLCVPW---KDHIGQYNEAGVALAI 188

Query: 195 FSLLTQVTW--LAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNVL 254
             L     W   +KDI+ +  D                  P +G SS + +Y        
Sbjct: 189 SLLKYFQGWSLWSKDIILVIFDD-----------------PVYGPSSFLTSY-------- 248

Query: 255 YEFEANHVTEKESLDDFK-RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 314
                +  T   S    K R+G++ A L +++   +E+  D L V  +A+NGQ+PNLDL 
Sbjct: 249 ----FDQTTPYISYTPLKIRSGSIQAGLSLELVT-TENNSDVLEVLYQATNGQLPNLDLF 308

Query: 315 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYV 374
           N ++ +      + ++++ +             +F  + G                S Y 
Sbjct: 309 NTISRIFMQHFNYPLRLQGY-------------DFHANSG----------------SSYT 368

Query: 375 DGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEF---ILRGG 434
               +L   +  QAV   T  H  F  Y+ID +T+ M  K         D F   + R G
Sbjct: 369 SRLKSLWMGMLTQAVSNVTSAHALFPQYRIDMLTLRMKVK---------DPFSFDMFRFG 428

Query: 435 RLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYA 494
           + IE   RS+NNLLE  HQSFF Y ++    F+S+G YM +  +L     A +  L +Y 
Sbjct: 429 QAIESTFRSLNNLLEHLHQSFFFYFILDHLHFISIGNYMPSILIL-----AASFMLGAYR 488

Query: 495 NNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQIPG 554
           +                             W+N  K+   + LW      L    C I  
Sbjct: 489 H-----------------------------WINHEKK---IDLWRPFSFWLFSIFCTIAA 543

Query: 555 Y---SPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 614
           Y   S +T   ++  L L+ L  + +I  + ++S  +      E      + + + FI  
Sbjct: 549 YYLVSSSTKITVFIFLYLM-LTFIGIIFSTFMTSEDA------ELVLSYDLMSKSLFIS- 543

Query: 615 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 674
            ++S +NF+ + + A L+V +  ++                R    L L F      + F
Sbjct: 609 -VVSTLNFSLSFVVAILLVPLQFISF---------------RFNRRLSLLFAVLTYFSTF 543

Query: 675 LFKAALQGFDNLHLGDFWNWMETLWAWNS 690
           +F  +L    N  L  FW W +    +NS
Sbjct: 669 IFLCSLSKILNGPLVPFWLWAKEYELFNS 543

BLAST of Cp4.1LG13g09220 vs. NCBI nr
Match: XP_023550991.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1401 bits (3626), Expect = 0.0
Identity = 714/714 (100.00%), Postives = 714/714 (100.00%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Cp4.1LG13g09220 vs. NCBI nr
Match: KAG7016207.1 (Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1383 bits (3579), Expect = 0.0
Identity = 702/714 (98.32%), Postives = 710/714 (99.44%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPK+KARPIVRLGIFLISHSIFFSVICFSAG+LALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGILALLLLPMFALNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRS+MIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAAL+IKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALIIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLTLEKIEPAAPANPDD LIVSLRSW+WLNAAKRVFVVHLWGAVVSLLPYFICQ
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVA NFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAWNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Cp4.1LG13g09220 vs. NCBI nr
Match: XP_022939757.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1381 bits (3574), Expect = 0.0
Identity = 703/714 (98.46%), Postives = 709/714 (99.30%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAINTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           +FHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 RFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGF+IKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANN NLTLEKIEPAAPANPDD LIVSLRSW+WLNAAKRVFVVHLWGAVVSLLPYFIC 
Sbjct: 481 SYANNSNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICP 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Cp4.1LG13g09220 vs. NCBI nr
Match: XP_022993328.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1376 bits (3561), Expect = 0.0
Identity = 703/714 (98.46%), Postives = 708/714 (99.16%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPK+KARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRS+MIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANN NLTLEKIEPAAPAN DD LIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFI Q
Sbjct: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Cp4.1LG13g09220 vs. NCBI nr
Match: KAG6578667.1 (Glycosylphosphatidylinositol anchor attachment 1 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1334 bits (3453), Expect = 0.0
Identity = 682/695 (98.13%), Postives = 689/695 (99.14%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MA TEKPK+KARPIVRLGIFLISHSIFFSVICFSAG+LALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAGTEKPKTKARPIVRLGIFLISHSIFFSVICFSAGILALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRS+MIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAAL+IKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALIIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLTLEKIEPAAPANPDD LIVSLRSW+WLNAAKRVFVVHLWGAVVSLLP FICQ
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPNFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVA NFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAWNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYL 695
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYL
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYL 695

BLAST of Cp4.1LG13g09220 vs. ExPASy TrEMBL
Match: A0A6J1FHQ4 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1381 bits (3574), Expect = 0.0
Identity = 703/714 (98.46%), Postives = 709/714 (99.30%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAINTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           +FHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 RFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGF+IKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANN NLTLEKIEPAAPANPDD LIVSLRSW+WLNAAKRVFVVHLWGAVVSLLPYFIC 
Sbjct: 481 SYANNSNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICP 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Cp4.1LG13g09220 vs. ExPASy TrEMBL
Match: A0A6J1K1W5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1376 bits (3561), Expect = 0.0
Identity = 703/714 (98.46%), Postives = 708/714 (99.16%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPK+KARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRS+MIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANN NLTLEKIEPAAPAN DD LIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFI Q
Sbjct: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of Cp4.1LG13g09220 vs. ExPASy TrEMBL
Match: A0A6J1FMH6 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1303 bits (3371), Expect = 0.0
Identity = 659/670 (98.36%), Postives = 665/670 (99.25%), Query Frame = 0

Query: 45  MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYIS 104
           MFA+NTYISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYIS
Sbjct: 1   MFAINTYISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYIS 60

Query: 105 NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAI 164
           NLGAEVNYHRFQPQSS+FHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAI
Sbjct: 61  NLGAEVNYHRFQPQSSRFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI 120

Query: 165 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRD 224
           VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRD
Sbjct: 121 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRD 180

Query: 225 YHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 284
           YHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH
Sbjct: 181 YHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 240

Query: 285 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES 344
           FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGF+IKIEKFWPLLDCKWLKFLGE FE+
Sbjct: 241 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFET 300

Query: 345 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMS 404
           IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMS
Sbjct: 301 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMS 360

Query: 405 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 464
           PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA
Sbjct: 361 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 420

Query: 465 FALLVAPLPAVAASLYSYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVV 524
           FALLVAPLPAVAASLYSYANN NLTLEKIEPAAPANPDD LIVSLRSW+WLNAAKRVFVV
Sbjct: 421 FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVV 480

Query: 525 HLWGAVVSLLPYFICQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQE 584
           HLWGAVVSLLPYFIC IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQE
Sbjct: 481 HLWGAVVSLLPYFICPIPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQE 540

Query: 585 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 644
           WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA
Sbjct: 541 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 600

Query: 645 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 704
           SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV
Sbjct: 601 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 660

Query: 705 LCTQILLHPC 714
           LCTQILLHPC
Sbjct: 661 LCTQILLHPC 670

BLAST of Cp4.1LG13g09220 vs. ExPASy TrEMBL
Match: A0A6J1JZW5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1299 bits (3361), Expect = 0.0
Identity = 660/670 (98.51%), Postives = 664/670 (99.10%), Query Frame = 0

Query: 45  MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYIS 104
           MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYIS
Sbjct: 1   MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYIS 60

Query: 105 NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAI 164
           NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAI
Sbjct: 61  NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI 120

Query: 165 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRD 224
           VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRD
Sbjct: 121 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRD 180

Query: 225 YHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 284
           YHTPSFGRS+MIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH
Sbjct: 181 YHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 240

Query: 285 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES 344
           FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES
Sbjct: 241 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES 300

Query: 345 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMS 404
           IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMS
Sbjct: 301 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 360

Query: 405 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 464
           PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA
Sbjct: 361 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 420

Query: 465 FALLVAPLPAVAASLYSYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVV 524
           FALLVAPLPAVAASLYSYANN NLTLEKIEPAAPAN DD LIVSLRSWKWLNAAKRVFVV
Sbjct: 421 FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVV 480

Query: 525 HLWGAVVSLLPYFICQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQE 584
           HLWGAVVSLLPYFI QIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQE
Sbjct: 481 HLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQE 540

Query: 585 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 644
           WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA
Sbjct: 541 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 600

Query: 645 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 704
           SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV
Sbjct: 601 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 660

Query: 705 LCTQILLHPC 714
           LCTQILLHPC
Sbjct: 661 LCTQILLHPC 670

BLAST of Cp4.1LG13g09220 vs. ExPASy TrEMBL
Match: A0A0A0KP05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1)

HSP 1 Score: 1291 bits (3340), Expect = 0.0
Identity = 656/714 (91.88%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEK K KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SAN+MLSG+EVSEAN+LVKD+KGLNSK GS+IFGSQQILAQYIS LGA+VNYHRF PQSS
Sbjct: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGI++DNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPV+TSLH+T
Sbjct: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240
           LSLGIAYSIFSLLTQVTWLAKDIVWL ADSRYGEYAAVSAWLRDYHTP FG+SS+IDT  
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL EFEAN VTEK  LDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLA HRQGFRIKIEKFWPLL+CKWLK LGE FESIGK IRSLN EWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
            ASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSG+KVRRD+FIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVST KFVSVGVYMIAFALLVAPLPAVAA+LY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540
           SYANNLNLT EK+EP A ANPDD LIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFICQ
Sbjct: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPT NSIIW LLSLL+LL+LSVILGSP SSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINF+TAELGAFLVVSMCLLAHPLKLDL AGNFKALSRAA NLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714
           LFK ALQGFDNLH+GDFWNWMETLWAWNSATFLYLGMVHLPCW+LCTQILLHPC
Sbjct: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 714

BLAST of Cp4.1LG13g09220 vs. TAIR 10
Match: AT5G19130.1 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 779.2 bits (2011), Expect = 2.7e-225
Identity = 414/715 (57.90%), Postives = 517/715 (72.31%), Query Frame = 0

Query: 7   PKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTML 66
           PK K RPIVRLGIFLI+HS  FSV+  +AGVLALLLLP+ A NTYISENALMPGSA +ML
Sbjct: 19  PKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSML 78

Query: 67  SGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLH 126
           S ++VS+ + LVKD+K             Q+++ +Y+S++GAEV+Y +F P+ +QFHPLH
Sbjct: 79  SNRDVSDGSKLVKDIKNFRLNHEGQGVEVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLH 138

Query: 127 FFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186
           FFS PDS  + +NVSC S G+N  GIIRAP+ DGKE+IVLVTPY+ +    +E LSLGI 
Sbjct: 139 FFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIV 198

Query: 187 YSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNV 246
            S+FSLL++VTWL+KDI+WL ADSRYG+Y  V+AWL +YH+PSF    + D   C E N 
Sbjct: 199 SSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSF---KVSDLLKCDEQN- 258

Query: 247 LYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306
                        + D+F+RAGTMAAALV+KV  RSE FED+LS+YAEASNGQMPNLDLI
Sbjct: 259 -------------TADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLI 318

Query: 307 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYV 366
           N+VNYLA HRQGF +K+EK   LL   WLK  GE FE++GK    LNP+W FG+ A+DY+
Sbjct: 319 NVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYL 378

Query: 367 DGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLI 426
           +G+ATLASSLY QA+GIPTGPHGAFRDYQ+DAIT+++SP+F    K R+ +F LRG RL+
Sbjct: 379 EGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLL 438

Query: 427 EGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY-----S 486
           EG +RSVNNLLEKFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     S
Sbjct: 439 EGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKS 498

Query: 487 YANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQI 546
             N+ +            NP +    + +SWKWL+AAK+VF +HL G +V+LLPYFICQ+
Sbjct: 499 LTNSTH------------NPTE----NFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQV 558

Query: 547 PG-YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 606
           PG +SPT  SI+W   S   LLI  V +  P  S  S       WA LK++T SAAFIGL
Sbjct: 559 PGQHSPTNRSIMWGTTSSSLLLITFVTM--PGCSPFSSRLHGTNWAILKSVTISAAFIGL 618

Query: 607 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 666
           CLMS+INFATAE+GA L+V  CL+A P+K  L +   K+L  A  ++VL  I FP + F 
Sbjct: 619 CLMSIINFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFA 678

Query: 667 LFKAAL-QGFDNLHL-GDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 714
           + K  L +G   L L G+FW W+E+LWAW SAT+LY+GMVHLPCW+LC  IL HP
Sbjct: 679 ISKGLLGEGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 698

BLAST of Cp4.1LG13g09220 vs. TAIR 10
Match: AT5G19130.2 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 778.5 bits (2009), Expect = 4.7e-225
Identity = 414/715 (57.90%), Postives = 518/715 (72.45%), Query Frame = 0

Query: 7   PKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTML 66
           PK K RPIVRLGIFLI+HS  FSV+  +AGVLALLLLP+ A NTYISENALMPGSA +ML
Sbjct: 19  PKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSML 78

Query: 67  SGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLH 126
           S ++VS+ + LVKD+K         +   Q+++ +Y+S++GAEV+Y +F P+ +QFHPLH
Sbjct: 79  SNRDVSDGSKLVKDIKNFRLNHEGQV---QKLIGKYMSDMGAEVSYQKFHPEGNQFHPLH 138

Query: 127 FFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186
           FFS PDS  + +NVSC S G+N  GIIRAP+ DGKE+IVLVTPY+ +    +E LSLGI 
Sbjct: 139 FFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIV 198

Query: 187 YSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYTCAETNV 246
            S+FSLL++VTWL+KDI+WL ADSRYG+Y  V+AWL +YH+PSF    + D   C E N 
Sbjct: 199 SSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSF---KVSDLLKCDEQN- 258

Query: 247 LYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306
                        + D+F+RAGTMAAALV+KV  RSE FED+LS+YAEASNGQMPNLDLI
Sbjct: 259 -------------TADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLI 318

Query: 307 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYV 366
           N+VNYLA HRQGF +K+EK   LL   WLK  GE FE++GK    LNP+W FG+ A+DY+
Sbjct: 319 NVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYL 378

Query: 367 DGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLI 426
           +G+ATLASSLY QA+GIPTGPHGAFRDYQ+DAIT+++SP+F    K R+ +F LRG RL+
Sbjct: 379 EGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLL 438

Query: 427 EGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY-----S 486
           EG +RSVNNLLEKFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     S
Sbjct: 439 EGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKS 498

Query: 487 YANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQI 546
             N+ +            NP +    + +SWKWL+AAK+VF +HL G +V+LLPYFICQ+
Sbjct: 499 LTNSTH------------NPTE----NFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQV 558

Query: 547 PG-YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 606
           PG +SPT  SI+W   S   LLI  V +  P  S  S       WA LK++T SAAFIGL
Sbjct: 559 PGQHSPTNRSIMWGTTSSSLLLITFVTM--PGCSPFSSRLHGTNWAILKSVTISAAFIGL 618

Query: 607 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 666
           CLMS+INFATAE+GA L+V  CL+A P+K  L +   K+L  A  ++VL  I FP + F 
Sbjct: 619 CLMSIINFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFA 678

Query: 667 LFKAAL-QGFDNLHL-GDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 714
           + K  L +G   L L G+FW W+E+LWAW SAT+LY+GMVHLPCW+LC  IL HP
Sbjct: 679 ISKGLLGEGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O432925.3e-2423.89Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606... [more]
Q9WTK32.0e-2324.59Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=1009... [more]
P390123.7e-1725.36GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Q9US484.8e-1722.50GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
Match NameE-valueIdentityDescription
XP_023550991.10.0100.00glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
KAG7016207.10.098.32Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma... [more]
XP_022939757.10.098.46glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
XP_022993328.10.098.46glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
KAG6578667.10.098.13Glycosylphosphatidylinositol anchor attachment 1 protein, partial [Cucurbita arg... [more]
Match NameE-valueIdentityDescription
A0A6J1FHQ40.098.46glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1K1W50.098.46glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1FMH60.098.36glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucu... [more]
A0A6J1JZW50.098.51glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucu... [more]
A0A0A0KP050.091.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G19130.12.7e-22557.90GPI transamidase component family protein / Gaa1-like family protein [more]
AT5G19130.24.7e-22557.90GPI transamidase component family protein / Gaa1-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007246GPI transamidase component Gaa1PFAMPF04114Gaa1coord: 145..706
e-value: 8.3E-109
score: 364.9
IPR007246GPI transamidase component Gaa1PIRSFPIRSF036762GAA1coord: 1..714
e-value: 1.5E-197
score: 655.9
IPR007246GPI transamidase component Gaa1PANTHERPTHR13304GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 1..711
NoneNo IPR availablePANTHERPTHR13304:SF0GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 1..711

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g09220.1Cp4.1LG13g09220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0042765 GPI-anchor transamidase complex
cellular_component GO:0016021 integral component of membrane