Cp4.1LG13g07730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g07730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCp4.1LG13: 8435602 .. 8440923 (-)
RNA-Seq ExpressionCp4.1LG13g07730
SyntenyCp4.1LG13g07730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTCCTGGAAGGCGATAATCGCGCCTAAAAAGAAATTGCATGGTATCGATGATGAGTTGCAGAAATTGCTTGATGCTAACATGGACGACGTGTCCGCCAGACGCCGAGCGCGGGCGGCGTTCAAAGGAATTCAACTCAGCATTGATCACATTCTGTTCAAGGTATTCTGGTTTACTGTTTCTTTCTTAGGTTCATGTTCGTGTTGTTATTAAGGCGTTGGTCGATCTTGGATAATTCGTTATGCCTTTATTTTTTTATTTTTTATTTTATTTTTTGTTTTTAGAGTATATTTTCTATGGGATTTCAATTACTTCTTTCGTTTTTCTTTTCAGACACCAAGCAATGGATTGAAGATTGAAGAGGTAATATCTAAAATTGAATATCTTTTGTTTTTTAATATGTTTAGAACTTGATCCTTTGTAGAAATCACTCATAATAATGAACTTGTTCACCTTCACTGTTATTTGAACAAGTAAAATCACATTTTCGTTCGAGTGGAATAGTTTTACTCTTTTACATGTTTATGAAGTTTTAAAATATCCAAACACCTCATAGATAGAGGTAGGAATTATCAAACGAATCACACTCCTTCGTATATGTCTAATCTTTTTAAAAACCTTAATCGTTGTGCCGTTTGGAGTATTTTTCCAGATGTATAAATATGTAGTTGTATTGCTGAGTCATTTGGCATGGAAAAGTAGTCGATTGCCATATAATGAGTCCTTTTTAAAGCTGTTTTGTTATCGGATCATTTGTCAATAAAAGTCGTAATGCTGAATCACCTTAAGGAAAAGTAAGATTCACATTACCTTGTTGATCAAATATCCCAAGACAAGAACACTTGTTTGAGATTTGAATCACTCCACAAGCTTGAATGATTCTGAACATGCAATCTAAACTATATAGAATTACAAAGGAACTTTGCTCTTGGCTAACGGAAAAGCACGAATGCTCTTTACATTTTTCAAATCTCCATTTTCTTTACATCTTCCTTGAAGTTTATATTGCATGATTGATGCCTTGGTTCATATCAGCCCATTTGTGCTTCTTGGAAGTCTATGCAATCTCGTTTTCAAGCTAAGACAATCAATCGTTTTTGTACAGTCATATGAAGTCAACTCCAGAGGACTGAGTATATTCTCAAAAAGCTGGATTCCAGTGACTGTCCGTCCTAAAGCAATGGTGTGTTATTGTCATGGCTATGGAGACACCTGCACATTTTTCTTTGAAGGCACGTGGCTCCTATCGAGTAGTATAAATGACCTAGTATGTCATTTTGTATACTATAATAGGAATTGACTTTTGTTTTGTGGTACTGTATTTCAGGAATCGCAAGAAAGTTCGCATTGTTAGGATATAGCGTCTTTGCTATGGATTATCCAGGTTTTGGTCTTTCAGAAGGTCTCCATGGCTTTATACCAAACTTCGACAGAATTGTAGATGACGTCATTGAACATTATTCCAAAGTGAAAGGTGAGTTAACTTGCCAAATTTTGTTGTTAACTAATCTGAAAATGATTGTAGTAGTGAGGAAGCTGGACAATTTTCCTTGAAATGGATCTTGAGGAATGATATTTTGCTTGATCCTTAAAGTTAATATTATACTTTATCTCCTGCTGCCTCAGGGCATCCGACCATTATTTGGAGGGATGATGTAGATTTCGACTGTAAGAATTTAGAGCAGCAAAATTCTGACATGCACGTAGTTATATTATATACCTCTCTGGAAGGTATCAGATATGATATGATAAGTCGCATGTGTTTGATGTCCTGTATAATTCTTGCATCTAAAGATATTGGGGGCTCTTTTGTGTGTGTAGTTCTGGGTAGCCCTTCTTCTGGTTTGGCGGTGTCCAAGCATGAATAGGATGAAAAACATAGAATAGCCCTTTGGACAGCGATTAGCATTGCTTGCAATAAAATTCTTGTATTGTTTCTGTGTTCTGTGTTATCTGGAATTTTTGTGGTTTATATGAGTACTAAGATCCAACTTGATTTCTTGTTGAAACCTTCATTTTAATATTGTCTGGCCTTTTGTTTACCTTGGTAACCTATGCCTATGTTGCCTCGTAGAATAGAAAGGATCTTACATATCGTCCAAGATTTCTTTATTTTTACTTTTAGAATAGATTTGTTTTGTAGAAAATACATTCTGTAAAATATTTGTTTTACTTGTGTCTCAACAGAAATCATTCAAACAAATAACAAAGTTTTTCAATGACAACCCTTTATTAGAAATTTATTTACTTGATTCCAAGGGCAGAGAACCCGGCGTTTAGTGCTCTCCCCAGCTTTCTCTTTGGGCAGTCTTTGGGGGGAGCTGTGGCTCTCAAGATACACCTAAAACAACCTCGTTCATGGGGTGGTGCAGTTCTTGTTGCGCCAATGTGCAAGGTATCTTTCTGAAAAAGATTATTCTAGAACACTTGCATTGTCTGTGGGGCTCCAATCAACTCAAAATTTCTTTCTAGATTTCAGATGATATGATCCCACCATGGGGAGTTTCACAAGTATTGATCGGAGCGTCTAAATTCCTTCCAAAGTACAAGTTGGTTCCACAGAAAGATTTGGCTGAGGCAGCTTTTAGAGACTTGAAGTATAGAGAAATGGTTTGCCCCTTGAGCAGAATGTTTATTTGAACAAAATGGTGGTATTACAAATAACAAATGCATGCCTTTGTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNAGATTTATTCATCCTATTTTTAAGTGAAGTGTTTAATCTATATGTTAGCAGATATGTTTATTTATTTTTTGCATTTCTGTGGATGCAGACAACATATAATGTCATTGCATACAAACATAAACCTCGCTTGCAGACTGCAGTAGAGATGTTGAGAACTACGCAAGAACTAGAACGCAGATTGAGAGAAGTATGCTATATTCACAACCTTTCAATTATGATATATATTTCTTTTCCTAATATTTTGCTCCACTGCTCTTCACCGCACATGTTTCCTTTTTATCAGGCAAGTAATGCATTAGCAAAGCTTATATAGGTGTAGATTAACGTAAGGATTATATATATATATATATATATGAGTCTAATTACTATGTTTAAGCCTCAGGCATGCGATATGATTAAAGTTGTTCAGTATGTTCAGTGTCTTGCAAGCTGCAAGCTGGTCTACTGCCTGCAAACAAGAAAAGGGTTCCAATCTAAGAGAATAAGAACAAATGAACAATTAGAAAAATATTTAGATATAGACATCCTAAGCAGGGGTAAATGTAGCCATGTAGAACACTCCACCTCTAAAGATTCCGTTATTTTTCTTAGTTCAGATTCCCATGAAAACAACAAAAAACCAACCCTCCATAAGACTCCACCCCTCCCCCTAGGGCCAAACAGACTCCAAGCCGTTCCTAATCGATCGAGTAAACTAGAAACTCCAGAGCAAATGGTCTTAGTCTTCAAATGCCTTCCTATTGAGAATGCACCATTGTAGCCTCCAAAAAGATGGAAGACTGCATTCTAAATCCAATCTGTAATACCTTGTCACACAGAATTTAACCTTCTTAGAAAATTTTTACATTCCATAATGTGGAGAATAATAGTGCGGAGAATAACATGGACTCAGACGCAGAGGGGCAGGGCTACACAAGTGATTAAAGGAAGAGCTACAACAGAACCCTTTTGAAGGAGCAAGGAAGTTTAATCTATTGATTACATCCTGTTCAAGTAAGGTTGTCTATAAACCTTGTTAGTTCCATAATTCGTTCTTATACAAAGCCTTTCTTTACTCTTCTTTCTCCATTCATAGGTTATCTGAATAAATCATAAACCATGTCTGATGACCCCACAACTCTTTTGTTCCCAAAACTCAAATTGTTGAAACCAAATTATTGACCATTCGAGGCCTCCAAAACATTTTTCCATGCCATCAGCTTGATAAGAAAAACTCTCATGTCCCAACTCCCACCTCTTTCAAATATTTCTCGAGGATAAAAGAAAATTTAGTCAAATACTTGGAACCAGGCATAGGCAAAGACCAGATGATCCAAAACATGATTCACTTAATGTGGTGGAATGAATCTGAAATCACATATTTAACATAATTTTGAATGTTAGACAAACAAGGGGTACAAGGGACCACACATCGCTCGAAGTCTTCTCAAGCATTCTCAAGCCACACCGACCTCGTAAAATAGCACACAACCTAGCTTGTTAGAAACTCTTGGATTCCTCTCCCTTGGTCTGGCCAAGCAAAAGCCAAACACCTCATAAAAGCCTCTCCAAACTGCACAATGCAAAATATCATAGCATATATCCTTGTAGTACTTGAGAGAGTTAATCTTCAACCATATCTTATCTCTCTCTCTTCCTTTGATCGAAGGATATATATTTGCGGTAAGTAAGAGTAATCATGTGATCAACTTTCACAGATGTCCTTGTTTTACGTCTCGTCAAGTCTTTCTCAACTGCTCCTATTCTCTTTGTTGACCAGTTAGAGAGCCTTATTGTTGACTCCGTGTTTGTACGTCTGATACATCATTGAAATGCTTAGTTTCTTGTTGGAAAAAGACCGTTCTATCACAGAATGCAATACAGTTTCTAATTTTCATTGAAGAGTCTAATTCCTTTAAAGATACACTGTGACTTTTATTGCAAATTCATTCCAGTTGTTTCAAGTACATAAAACACAATTTCAAGAACTAAATCAGTTTATTGAGATGTCTTCTAGGTTTCCCTGCCAATATTGATCCTGCATGGGGAGGCTGATACTGTAACCGACCCATCGGTAAGCAAGGCGTTGTACGAGAAATCAAGCAGTACAGATAGGAAGTTAATACTTTACGAAGAGGCATGGCATTCTCTTTTAGAGGGTGAGCCAGATGAAATTATAGTCCAGGTATTAGATGACATCATCTCATGGATGGACGAACGCTGCAAGTAACGGTTTTGGTCACCGACTTATCGACATTCTTTCGTGTATGATTTGGTGGGGAAAGAAAGGGAAAGAAAATGCCATGGAGAAGAGTTTGGAAATTATGTTATGGGCTTACAGCGTCAGGAAGCTGTTGAAGTAATGTATTAATAAAAATTGAATTGTAACAGGCGGACACTTTGAAACTAGACATCATTGCTTCTGTTTATGGCTCCATAAATCTCACTTGGTTTGGGTGAAAGAAAGCTTAAGGGTATGAGTTCAAGAACCAGCCACAAGCTATTGAAGGGTCTTTCTTTCATAATTTGAATGGTTATATTTTTTATATTTTTTATTATTTTTATTTTTTGAAAAAATCATTGTTCTCAAGACATTTTTTTAATTAAGTAATTA

mRNA sequence

ATGAGCTCCTGGAAGGCGATAATCGCGCCTAAAAAGAAATTGCATGGTATCGATGATGAGTTGCAGAAATTGCTTGATGCTAACATGGACGACGTGTCCGCCAGACGCCGAGCGCGGGCGGCGTTCAAAGGAATTCAACTCAGCATTGATCACATTCTGTTCAAGGTATTCTGGTTTACTGTTTCTTTCTTAGGAATCGCAAGAAAGTTCGCATTGTTAGGATATAGCGTCTTTGCTATGGATTATCCAGGTTTTGGTCTTTCAGAAGGTCTCCATGGCTTTATACCAAACTTCGACAGAATTGTAGATGACGTCATTGAACATTATTCCAAAGTGAAAGGGCATCCGACCATTATTTGGAGGGATGATGTAGATTTCGACTGTAAGAATTTAGAGCAGCAAAATTCTGACATGCACAAATTTATTTACTTGATTCCAAGGGCAGAGAACCCGGCGTTTAGTGCTCTCCCCAGCTTTCTCTTTGGGCAGTCTTTGGGGGGAGCTGTGGCTCTCAAGATACACCTAAAACAACCTCGTTCATGGGGTGGTGCAGTTCTTGTTGCGCCAATGTGCAAGATTTCAGATGATATGATCCCACCATGGGGAGTTTCACAAGTATTGATCGGAGCGTCTAAATTCCTTCCAAAGTACAAGTTGGTTCCACAGAAAGATTTGGCTGAGGCAGCTTTTAGAGACTTGAAGTATAGAGAAATGACAACATATAATGTCATTGCATACAAACATAAACCTCGCTTGCAGACTGCAGTAGAGATGTTGAGAACTACGCAAGAACTAGAACGCAGATTGAGAGAAGTTTCCCTGCCAATATTGATCCTGCATGGGGAGGCTGATACTGTAACCGACCCATCGGTAAGCAAGGCGTTGTACGAGAAATCAAGCAGTACAGATAGGAAGTTAATACTTTACGAAGAGGCATGGCATTCTCTTTTAGAGGGTGAGCCAGATGAAATTATAGTCCAGGTATTAGATGACATCATCTCATGGATGGACGAACGCTGCAAGTAACGGTTTTGGTCACCGACTTATCGACATTCTTTCGTGTATGATTTGGTGGGGAAAGAAAGGGAAAGAAAATGCCATGGAGAAGAGTTTGGAAATTATGTTATGGGCTTACAGCGTCAGGAAGCTGTTGAAGTAATGTATTAATAAAAATTGAATTGTAACAGGCGGACACTTTGAAACTAGACATCATTGCTTCTGTTTATGGCTCCATAAATCTCACTTGGTTTGGGTGAAAGAAAGCTTAAGGGTATGAGTTCAAGAACCAGCCACAAGCTATTGAAGGGTCTTTCTTTCATAATTTGAATGGTTATATTTTTTATATTTTTTATTATTTTTATTTTTTGAAAAAATCATTGTTCTCAAGACATTTTTTTAATTAAGTAATTA

Coding sequence (CDS)

ATGAGCTCCTGGAAGGCGATAATCGCGCCTAAAAAGAAATTGCATGGTATCGATGATGAGTTGCAGAAATTGCTTGATGCTAACATGGACGACGTGTCCGCCAGACGCCGAGCGCGGGCGGCGTTCAAAGGAATTCAACTCAGCATTGATCACATTCTGTTCAAGGTATTCTGGTTTACTGTTTCTTTCTTAGGAATCGCAAGAAAGTTCGCATTGTTAGGATATAGCGTCTTTGCTATGGATTATCCAGGTTTTGGTCTTTCAGAAGGTCTCCATGGCTTTATACCAAACTTCGACAGAATTGTAGATGACGTCATTGAACATTATTCCAAAGTGAAAGGGCATCCGACCATTATTTGGAGGGATGATGTAGATTTCGACTGTAAGAATTTAGAGCAGCAAAATTCTGACATGCACAAATTTATTTACTTGATTCCAAGGGCAGAGAACCCGGCGTTTAGTGCTCTCCCCAGCTTTCTCTTTGGGCAGTCTTTGGGGGGAGCTGTGGCTCTCAAGATACACCTAAAACAACCTCGTTCATGGGGTGGTGCAGTTCTTGTTGCGCCAATGTGCAAGATTTCAGATGATATGATCCCACCATGGGGAGTTTCACAAGTATTGATCGGAGCGTCTAAATTCCTTCCAAAGTACAAGTTGGTTCCACAGAAAGATTTGGCTGAGGCAGCTTTTAGAGACTTGAAGTATAGAGAAATGACAACATATAATGTCATTGCATACAAACATAAACCTCGCTTGCAGACTGCAGTAGAGATGTTGAGAACTACGCAAGAACTAGAACGCAGATTGAGAGAAGTTTCCCTGCCAATATTGATCCTGCATGGGGAGGCTGATACTGTAACCGACCCATCGGTAAGCAAGGCGTTGTACGAGAAATCAAGCAGTACAGATAGGAAGTTAATACTTTACGAAGAGGCATGGCATTCTCTTTTAGAGGGTGAGCCAGATGAAATTATAGTCCAGGTATTAGATGACATCATCTCATGGATGGACGAACGCTGCAAGTAA

Protein sequence

MSSWKAIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFKVFWFTVSFLGIARKFALLGYSVFAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIVQVLDDIISWMDERCK
Homology
BLAST of Cp4.1LG13g07730 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 6.3e-31
Identity = 88/283 (31.10%), Postives = 140/283 (49.47%), Query Frame = 0

Query: 63  FLGIARKFALLGYSVFAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRD 122
           F  I   F+  GY+VFA D  G G S+G+  ++ + +++    +  +  V          
Sbjct: 81  FQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHV---------- 140

Query: 123 DVDFDCKNLEQQNSDMHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLK-QPRSW 182
                                   R  +P +  LP+FLFG+S+GG V L ++ + +P +W
Sbjct: 141 ------------------------RCSDP-YKDLPAFLFGESMGGLVTLLMYFQSEPETW 200

Query: 183 GGAVLVAPMCKISDDMIPPWG---VSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREM 242
            G +  AP+  I +DM P         +L G +     +  +P   +   A +D +  ++
Sbjct: 201 TGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLA---DTWAAMPDNKMVGKAIKDPEKLKI 260

Query: 243 TTYNVIAYKHKPRLQTAVEMLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEK 302
              N   Y  KPR+ T  E+LR TQ ++    +V++P+   HG AD VT P+ SK LYEK
Sbjct: 261 IASNPQRYTGKPRVGTMRELLRKTQYVQENFGKVTIPVFTAHGTADGVTCPTSSKLLYEK 320

Query: 303 SSSTDRKLILYEEAWHSLLEGEPDEIIVQVLDDIISWMDERCK 342
           +SS D+ L +YE  +HSL++GEPDE    VL D+  W+DE+ K
Sbjct: 321 ASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMREWIDEKVK 325

BLAST of Cp4.1LG13g07730 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-14
Identity = 69/268 (25.75%), Postives = 118/268 (44.03%), Query Frame = 0

Query: 77  VFAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKV-KGHPTIIWRDDVDFDCKNLEQQN 136
           VFA D+ G G SEG    + +F   V DV++H   + K +P +                 
Sbjct: 72  VFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDV----------------- 131

Query: 137 SDMHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISD 196
                                P FL G S+GGA+++ +  ++P  + G VL++P+     
Sbjct: 132 ---------------------PIFLLGHSMGGAISILVAAERPTYFSGMVLISPLV---- 191

Query: 197 DMIPPWGVSQVLIGASKFLPKYKLVPQKDLA--EAAFRDLKYREMTTYNVIAYKHKPRLQ 256
            +  P   S + + A+K L    ++P   L   +++       E+  YN      +  L+
Sbjct: 192 -LANPESASTLKVLAAKLL--NFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLK 251

Query: 257 T--AVEMLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEE 316
               +++L     +ER +  ++LP L+L G AD + D   +  L E S S D+ L +YE 
Sbjct: 252 VCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEG 293

Query: 317 AWHSLLEGEPDEIIVQVLDDIISWMDER 340
           A+H +L  E  E+   VL ++ SW+  R
Sbjct: 312 AYH-VLHRELPEVTNSVLHEVNSWVSHR 293

BLAST of Cp4.1LG13g07730 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 5.3e-14
Identity = 68/276 (24.64%), Postives = 117/276 (42.39%), Query Frame = 0

Query: 66  IARKFALLGYSVFAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVD 125
           +AR    L   VFA D+ G G SEG    + +F   V DV++H   ++            
Sbjct: 61  LARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQ------------ 120

Query: 126 FDCKNLEQQNSDMHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAV 185
                                      +  LP FL G S+GGA+A+    ++P  + G V
Sbjct: 121 -------------------------KDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMV 180

Query: 186 LVAPMCKISDDMIPPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIA 245
           L++P+   + +    + V    +  +  LP   L P     +++       E+  YN   
Sbjct: 181 LISPLVLANPESATTFKVLAAKV-LNLVLPNLSLGP----IDSSVLSRNKTEVDIYNSDP 240

Query: 246 YKHKPRLQT--AVEMLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTD 305
              +  L+    +++L     +ER L ++++P L+L G AD + D   +  L E + S D
Sbjct: 241 LICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQD 293

Query: 306 RKLILYEEAWHSLLEGEPDEIIVQVLDDIISWMDER 340
           + L +YE A+H +L  E  E+   V  +I  W+ +R
Sbjct: 301 KTLKIYEGAYH-VLHKELPEVTNSVFHEINMWVSQR 293

BLAST of Cp4.1LG13g07730 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 9.1e-14
Identity = 69/279 (24.73%), Postives = 122/279 (43.73%), Query Frame = 0

Query: 66  IARKFALLGYSVFAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKV-KGHPTIIWRDDV 125
           +A+    L   VFA D+ G G SEG    + +F   V D+++H + V K +P +      
Sbjct: 61  LAQMLKRLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEV------ 120

Query: 126 DFDCKNLEQQNSDMHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGA 185
                                           P FL G S+GGA+++    ++P  + G 
Sbjct: 121 --------------------------------PVFLLGHSMGGAISILAAAERPTHFSGM 180

Query: 186 VLVAPMCKISDDMIPPWGVSQVLIGASKFLPKYKLVPQKDLA--EAAFRDLKYREMTTYN 245
           +L++P+      +  P   S + + A+K L    ++P   L   +++       E+  YN
Sbjct: 181 ILISPLI-----LANPESASTLKVLAAKLL--NFVLPNISLGRIDSSVLSRNKSEVDLYN 240

Query: 246 --VIAYKHKPRLQTAVEMLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSS 305
              +      ++   +++L     +ER +  ++LP L+L G AD + D   +  L E S 
Sbjct: 241 SDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSP 293

Query: 306 STDRKLILYEEAWHSLLEGEPDEIIVQVLDDIISWMDER 340
           S D+ L +YE A+H +L  E  E+   VL +I +W+  R
Sbjct: 301 SQDKTLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHR 293

BLAST of Cp4.1LG13g07730 vs. ExPASy Swiss-Prot
Match: P28321 (Monoglyceride lipase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJU3 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 9.8e-08
Identity = 45/192 (23.44%), Postives = 82/192 (42.71%), Query Frame = 0

Query: 156 LPSFLFGQSLGGAVALKIHL--KQPRSWGGAVLVAPMCKISDDMIPPWGVSQVLIGASKF 215
           +P F++G S+GG + L      K      G +   P+  +    +       +    +KF
Sbjct: 115 IPLFMWGHSMGGGICLNYACQGKHKNEISGYIGSGPLIILHPHTMYNKPTQIIAPLLAKF 174

Query: 216 LPKYKLVPQKDL-------AEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELE 275
           LP+ ++    DL       A  AF       +  Y      H   +Q   ++ +      
Sbjct: 175 LPRVRIDTGLDLKGITSDKAYRAFLGSDPMSVPLYGSFRQIH-DFMQRGAKLYKNENNYI 234

Query: 276 RRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIV 335
           ++      P++I+HG+ DT+ DP  S+   +   S D++L LY  A HS+   E D++  
Sbjct: 235 QKNFAKDKPVIIMHGQDDTINDPKGSEKFIQDCPSADKELKLYPGARHSIFSLETDKVFN 294

Query: 336 QVLDDIISWMDE 339
            V +D+  W+D+
Sbjct: 295 TVFNDMKQWLDK 305

BLAST of Cp4.1LG13g07730 vs. NCBI nr
Match: KAG7016061.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 562 bits (1449), Expect = 1.47e-199
Identity = 297/361 (82.27%), Postives = 299/361 (82.83%), Query Frame = 0

Query: 1   MSSWKAIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFKV---- 60
           MSSWKAIIAPKKKLHGIDDELQKLLDANMDDV+ARRRARAAFKGIQLSIDHILFK     
Sbjct: 49  MSSWKAIIAPKKKLHGIDDELQKLLDANMDDVAARRRARAAFKGIQLSIDHILFKTPSNG 108

Query: 61  -----FWFTV-----------SFLGIARKFALLGYSVFAMDYPGFGLSEGLHGFIPNFDR 120
                 W  +           S   IARKFALLGYSVFAMDYPGFGLSEGLHGFIPNFDR
Sbjct: 109 LKIEEQWCVIVMAMETPAHFSSKARIARKFALLGYSVFAMDYPGFGLSEGLHGFIPNFDR 168

Query: 121 IVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFIYLIPRAENPAFSALPSFL 180
           IVDDVIEHYSKVK                                   ENPAFSALPSFL
Sbjct: 169 IVDDVIEHYSKVK-----------------------------------ENPAFSALPSFL 228

Query: 181 FGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVSQVLIGASKFLPKYKLV 240
           FGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVSQVLIGASKFLPKYKLV
Sbjct: 229 FGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVSQVLIGASKFLPKYKLV 288

Query: 241 PQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELERRLREVSLPILILH 300
           PQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELERRLREVSLPILILH
Sbjct: 289 PQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELERRLREVSLPILILH 348

Query: 301 GEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIVQVLDDIISWMDERC 341
           GEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIVQVLDDIISWMDERC
Sbjct: 349 GEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIVQVLDDIISWMDERC 374

BLAST of Cp4.1LG13g07730 vs. NCBI nr
Match: KAG6578497.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 528 bits (1360), Expect = 1.57e-185
Identity = 286/370 (77.30%), Postives = 289/370 (78.11%), Query Frame = 0

Query: 1   MSSWKAIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK----- 60
           MSSWKAIIAPKKKLHGIDDELQKLLDANMDDV+ARRRARAAFKGIQLSIDHILFK     
Sbjct: 49  MSSWKAIIAPKKKLHGIDDELQKLLDANMDDVAARRRARAAFKGIQLSIDHILFKTPSNG 108

Query: 61  ---------------VF---WFTVS--------------------FLGIARKFALLGYSV 120
                          +F   W  V+                    F GIARKFALLGYSV
Sbjct: 109 LKIEESYDVNSRGLSIFSKSWIPVTVRPKAMVCYCHGYGDTCTFFFEGIARKFALLGYSV 168

Query: 121 FAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSD 180
           FAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVK                        
Sbjct: 169 FAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVK------------------------ 228

Query: 181 MHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDM 240
                      ENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDM
Sbjct: 229 -----------ENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDM 288

Query: 241 IPPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVE 300
           IPPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVE
Sbjct: 289 IPPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVE 348

Query: 301 MLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLL 327
           MLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLL
Sbjct: 349 MLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLL 383

BLAST of Cp4.1LG13g07730 vs. NCBI nr
Match: XP_038890284.1 (caffeoylshikimate esterase isoform X1 [Benincasa hispida])

HSP 1 Score: 482 bits (1240), Expect = 2.17e-167
Identity = 250/379 (65.96%), Postives = 280/379 (73.88%), Query Frame = 0

Query: 6   AIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK---------- 65
           AI+AP+KKL G+DDELQKLLDANMD V+ RRRAR AFK IQL +DHILFK          
Sbjct: 55  AIMAPRKKLQGVDDELQKLLDANMDGVATRRRAREAFKNIQLGVDHILFKTPSDGLKMEE 114

Query: 66  ----------VF---WF--------------------TVSFLGIARKFALLGYSVFAMDY 125
                     +F   W                     T  F GIARK AL GY VF+MDY
Sbjct: 115 TYEVNSRGLSIFSKSWIPETVRPKAMVCYCHGYGDTCTFFFEGIARKLALSGYGVFSMDY 174

Query: 126 PGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFI 185
           PGFGLSEGLHGFIPNFDRIVDDVIEHYSKVK                             
Sbjct: 175 PGFGLSEGLHGFIPNFDRIVDDVIEHYSKVK----------------------------- 234

Query: 186 YLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWG 245
                 ENPA+SALPSFLFGQSLGGAVALK+HLKQPRSW GAVLVAPMCKI+DDM+PPW 
Sbjct: 235 ------ENPAYSALPSFLFGQSLGGAVALKVHLKQPRSWSGAVLVAPMCKIADDMVPPWA 294

Query: 246 VSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTT 305
           ++QVLIG SKFLPKYKLVPQKDLAEAAFRDLKYRE+T YNVIAYKHKPRLQTAVEML+TT
Sbjct: 295 LAQVLIGVSKFLPKYKLVPQKDLAEAAFRDLKYRELTAYNVIAYKHKPRLQTAVEMLKTT 354

Query: 306 QELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPD 341
           QE+ERRL+E+SLP+LILHGEAD VTDPSVSK LYEK+SS+D+K+ LY++A+HSLLEGEPD
Sbjct: 355 QEIERRLKEISLPLLILHGEADIVTDPSVSKLLYEKASSSDKKIKLYKDAYHSLLEGEPD 398

BLAST of Cp4.1LG13g07730 vs. NCBI nr
Match: XP_038890285.1 (caffeoylshikimate esterase isoform X2 [Benincasa hispida])

HSP 1 Score: 479 bits (1232), Expect = 4.89e-167
Identity = 248/377 (65.78%), Postives = 278/377 (73.74%), Query Frame = 0

Query: 8   IAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK------------ 67
           +AP+KKL G+DDELQKLLDANMD V+ RRRAR AFK IQL +DHILFK            
Sbjct: 1   MAPRKKLQGVDDELQKLLDANMDGVATRRRAREAFKNIQLGVDHILFKTPSDGLKMEETY 60

Query: 68  --------VF---WF--------------------TVSFLGIARKFALLGYSVFAMDYPG 127
                   +F   W                     T  F GIARK AL GY VF+MDYPG
Sbjct: 61  EVNSRGLSIFSKSWIPETVRPKAMVCYCHGYGDTCTFFFEGIARKLALSGYGVFSMDYPG 120

Query: 128 FGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFIYL 187
           FGLSEGLHGFIPNFDRIVDDVIEHYSKVK                               
Sbjct: 121 FGLSEGLHGFIPNFDRIVDDVIEHYSKVK------------------------------- 180

Query: 188 IPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVS 247
               ENPA+SALPSFLFGQSLGGAVALK+HLKQPRSW GAVLVAPMCKI+DDM+PPW ++
Sbjct: 181 ----ENPAYSALPSFLFGQSLGGAVALKVHLKQPRSWSGAVLVAPMCKIADDMVPPWALA 240

Query: 248 QVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQE 307
           QVLIG SKFLPKYKLVPQKDLAEAAFRDLKYRE+T YNVIAYKHKPRLQTAVEML+TTQE
Sbjct: 241 QVLIGVSKFLPKYKLVPQKDLAEAAFRDLKYRELTAYNVIAYKHKPRLQTAVEMLKTTQE 300

Query: 308 LERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEI 341
           +ERRL+E+SLP+LILHGEAD VTDPSVSK LYEK+SS+D+K+ LY++A+HSLLEGEPDE+
Sbjct: 301 IERRLKEISLPLLILHGEADIVTDPSVSKLLYEKASSSDKKIKLYKDAYHSLLEGEPDEV 342

BLAST of Cp4.1LG13g07730 vs. NCBI nr
Match: XP_008459473.1 (PREDICTED: caffeoylshikimate esterase isoform X1 [Cucumis melo])

HSP 1 Score: 474 bits (1220), Expect = 2.46e-164
Identity = 247/380 (65.00%), Postives = 279/380 (73.42%), Query Frame = 0

Query: 5   KAIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK--------- 64
           K I+AP+KKL G+DDELQKLLDANMD V+ARRRAR AFKGIQL IDHILFK         
Sbjct: 55  KVIMAPRKKLQGVDDELQKLLDANMDGVAARRRAREAFKGIQLGIDHILFKTPSDGLKME 114

Query: 65  -----------VF---WF--------------------TVSFLGIARKFALLGYSVFAMD 124
                      +F   W                     T  F GIARK A+ GY VF+MD
Sbjct: 115 ETYEVNSRGLSIFSKSWIPETVRPKAMVYYCHGYGDTCTFFFEGIARKLAMSGYGVFSMD 174

Query: 125 YPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKF 184
           YPGFGLSEGLHGFIP+FDRIVDDVIE YSKVK                            
Sbjct: 175 YPGFGLSEGLHGFIPSFDRIVDDVIERYSKVK---------------------------- 234

Query: 185 IYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPW 244
                  ENPAFSALPSFLFGQSLGGAVALK+HLKQPRSW GAVLVAPMCKI+DDM+PPW
Sbjct: 235 -------ENPAFSALPSFLFGQSLGGAVALKVHLKQPRSWSGAVLVAPMCKIADDMVPPW 294

Query: 245 GVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRT 304
            ++QVLIG SKFLPKYKLVPQKDLAE AFRDLKYRE+T YNVIAYK KPRLQTAVEML+T
Sbjct: 295 ALAQVLIGISKFLPKYKLVPQKDLAELAFRDLKYRELTAYNVIAYKDKPRLQTAVEMLKT 354

Query: 305 TQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEP 341
           TQE+ERRL+E+SLP+LILHGEADTVTDPSVSK LYEK+SS+D+K+ LY++A+HSLLEGEP
Sbjct: 355 TQEIERRLKEISLPLLILHGEADTVTDPSVSKVLYEKASSSDKKIKLYKDAYHSLLEGEP 399

BLAST of Cp4.1LG13g07730 vs. ExPASy TrEMBL
Match: A0A1S3CBH4 (caffeoylshikimate esterase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498599 PE=4 SV=1)

HSP 1 Score: 474 bits (1220), Expect = 1.19e-164
Identity = 247/380 (65.00%), Postives = 279/380 (73.42%), Query Frame = 0

Query: 5   KAIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK--------- 64
           K I+AP+KKL G+DDELQKLLDANMD V+ARRRAR AFKGIQL IDHILFK         
Sbjct: 55  KVIMAPRKKLQGVDDELQKLLDANMDGVAARRRAREAFKGIQLGIDHILFKTPSDGLKME 114

Query: 65  -----------VF---WF--------------------TVSFLGIARKFALLGYSVFAMD 124
                      +F   W                     T  F GIARK A+ GY VF+MD
Sbjct: 115 ETYEVNSRGLSIFSKSWIPETVRPKAMVYYCHGYGDTCTFFFEGIARKLAMSGYGVFSMD 174

Query: 125 YPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKF 184
           YPGFGLSEGLHGFIP+FDRIVDDVIE YSKVK                            
Sbjct: 175 YPGFGLSEGLHGFIPSFDRIVDDVIERYSKVK---------------------------- 234

Query: 185 IYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPW 244
                  ENPAFSALPSFLFGQSLGGAVALK+HLKQPRSW GAVLVAPMCKI+DDM+PPW
Sbjct: 235 -------ENPAFSALPSFLFGQSLGGAVALKVHLKQPRSWSGAVLVAPMCKIADDMVPPW 294

Query: 245 GVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRT 304
            ++QVLIG SKFLPKYKLVPQKDLAE AFRDLKYRE+T YNVIAYK KPRLQTAVEML+T
Sbjct: 295 ALAQVLIGISKFLPKYKLVPQKDLAELAFRDLKYRELTAYNVIAYKDKPRLQTAVEMLKT 354

Query: 305 TQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEP 341
           TQE+ERRL+E+SLP+LILHGEADTVTDPSVSK LYEK+SS+D+K+ LY++A+HSLLEGEP
Sbjct: 355 TQEIERRLKEISLPLLILHGEADTVTDPSVSKVLYEKASSSDKKIKLYKDAYHSLLEGEP 399

BLAST of Cp4.1LG13g07730 vs. ExPASy TrEMBL
Match: A0A6J1JHR7 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111487110 PE=4 SV=1)

HSP 1 Score: 473 bits (1216), Expect = 3.65e-164
Identity = 247/383 (64.49%), Postives = 280/383 (73.11%), Query Frame = 0

Query: 2   SSWKAIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK------ 61
           SS KA++APK KLHG++D+L KLLDANMDDVS RRR R AFK IQL IDHILFK      
Sbjct: 44  SSRKAVMAPKMKLHGVNDKLLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGL 103

Query: 62  --------------VF---WF--------------------TVSFLGIARKFALLGYSVF 121
                         +F   W                     T  F GIARK AL GY VF
Sbjct: 104 KMEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIARKLALSGYGVF 163

Query: 122 AMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDM 181
           +MDYPGFGLSEGLHGFIP+FD+IVDDVIEHYSKVK                         
Sbjct: 164 SMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVK------------------------- 223

Query: 182 HKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMI 241
                     ENP FSALPSFLFGQSLGGAVALK+HLKQP SW GAVLVAPMCKI+DDM+
Sbjct: 224 ----------ENPEFSALPSFLFGQSLGGAVALKVHLKQPHSWTGAVLVAPMCKIADDMV 283

Query: 242 PPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEM 301
           PPW ++Q+LIG +KFLPKYKLVPQK+LAEAAFRD+K+RE+T YNVIAYKHKPRLQTAVEM
Sbjct: 284 PPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYKHKPRLQTAVEM 343

Query: 302 LRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLE 341
           LRTTQE+ERRL+EVSLP+LILHGEADTVTDPSVSKALYEK+SS+D+ + LY++A+HSLLE
Sbjct: 344 LRTTQEIERRLKEVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLE 391

BLAST of Cp4.1LG13g07730 vs. ExPASy TrEMBL
Match: A0A1S4E2J5 (caffeoylshikimate esterase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498599 PE=4 SV=1)

HSP 1 Score: 471 bits (1211), Expect = 3.70e-164
Identity = 245/377 (64.99%), Postives = 277/377 (73.47%), Query Frame = 0

Query: 8   IAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK------------ 67
           +AP+KKL G+DDELQKLLDANMD V+ARRRAR AFKGIQL IDHILFK            
Sbjct: 1   MAPRKKLQGVDDELQKLLDANMDGVAARRRAREAFKGIQLGIDHILFKTPSDGLKMEETY 60

Query: 68  --------VF---WF--------------------TVSFLGIARKFALLGYSVFAMDYPG 127
                   +F   W                     T  F GIARK A+ GY VF+MDYPG
Sbjct: 61  EVNSRGLSIFSKSWIPETVRPKAMVYYCHGYGDTCTFFFEGIARKLAMSGYGVFSMDYPG 120

Query: 128 FGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFIYL 187
           FGLSEGLHGFIP+FDRIVDDVIE YSKVK                               
Sbjct: 121 FGLSEGLHGFIPSFDRIVDDVIERYSKVK------------------------------- 180

Query: 188 IPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVS 247
               ENPAFSALPSFLFGQSLGGAVALK+HLKQPRSW GAVLVAPMCKI+DDM+PPW ++
Sbjct: 181 ----ENPAFSALPSFLFGQSLGGAVALKVHLKQPRSWSGAVLVAPMCKIADDMVPPWALA 240

Query: 248 QVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQE 307
           QVLIG SKFLPKYKLVPQKDLAE AFRDLKYRE+T YNVIAYK KPRLQTAVEML+TTQE
Sbjct: 241 QVLIGISKFLPKYKLVPQKDLAELAFRDLKYRELTAYNVIAYKDKPRLQTAVEMLKTTQE 300

Query: 308 LERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEI 341
           +ERRL+E+SLP+LILHGEADTVTDPSVSK LYEK+SS+D+K+ LY++A+HSLLEGEPDE+
Sbjct: 301 IERRLKEISLPLLILHGEADTVTDPSVSKVLYEKASSSDKKIKLYKDAYHSLLEGEPDEV 342

BLAST of Cp4.1LG13g07730 vs. ExPASy TrEMBL
Match: A0A6J1GRR0 (caffeoylshikimate esterase OS=Cucurbita moschata OX=3662 GN=LOC111456899 PE=4 SV=1)

HSP 1 Score: 471 bits (1211), Expect = 2.03e-163
Identity = 246/382 (64.40%), Postives = 279/382 (73.04%), Query Frame = 0

Query: 2   SSWKAIIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK------ 61
           SS KA++APK KLHG++DEL KLLDANMDDVS RRR R AFK IQL IDHILFK      
Sbjct: 44  SSRKAVMAPKMKLHGVNDELLKLLDANMDDVSTRRRVREAFKEIQLGIDHILFKTPSDGL 103

Query: 62  --------------VF---WF--------------------TVSFLGIARKFALLGYSVF 121
                         +F   W                     T  F GIARK AL GY VF
Sbjct: 104 KTEESYEVNSRGLSIFSKCWIPETVRPKAMVCYCHGYGDTCTFFFEGIARKLALSGYGVF 163

Query: 122 AMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDM 181
           +MDYPGFGLSEGLHGFIP+FD+IVDDVIEHYSKVK                         
Sbjct: 164 SMDYPGFGLSEGLHGFIPSFDKIVDDVIEHYSKVK------------------------- 223

Query: 182 HKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMI 241
                     ENP FSALPSFLFGQSLGGAVALK+HLKQP +W GAVLVAPMCKI+DDM+
Sbjct: 224 ----------ENPEFSALPSFLFGQSLGGAVALKVHLKQPHAWTGAVLVAPMCKIADDMV 283

Query: 242 PPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEM 301
           PPW ++Q+LIG +KFLPKYKLVPQK+LAEAAFRD+K+RE+T YNVIAYKHKPRLQTAVEM
Sbjct: 284 PPWALAQILIGVAKFLPKYKLVPQKNLAEAAFRDMKHRELTAYNVIAYKHKPRLQTAVEM 343

Query: 302 LRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLE 340
           LRTTQE+ERRL+EVSLP+LILHGEADTVTDPSVSKALYEK+SS+D+ + LY++A+HSLLE
Sbjct: 344 LRTTQEIERRLKEVSLPLLILHGEADTVTDPSVSKALYEKASSSDKSIKLYKDAYHSLLE 390

BLAST of Cp4.1LG13g07730 vs. ExPASy TrEMBL
Match: A0A0A0KSP3 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G644590 PE=4 SV=1)

HSP 1 Score: 466 bits (1198), Expect = 3.51e-162
Identity = 243/375 (64.80%), Postives = 274/375 (73.07%), Query Frame = 0

Query: 10  PKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFK-------------- 69
           P+KKL G+DDEL KLLDANMD V+ARRRAR AFK IQL +DHILFK              
Sbjct: 3   PRKKLQGVDDELLKLLDANMDGVAARRRAREAFKRIQLGVDHILFKTPSDGFKTEETYEV 62

Query: 70  ------VF---WF--------------------TVSFLGIARKFALLGYSVFAMDYPGFG 129
                 +F   W                     T  F GIARK AL GY VF+MDYPGFG
Sbjct: 63  NSRGLSIFSKCWIPETVRPKAMVYYCHGYGDTCTFFFEGIARKLALSGYGVFSMDYPGFG 122

Query: 130 LSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFIYLIP 189
           LSEGLHGFIP+FDRIVDDVIE YSKVK                                 
Sbjct: 123 LSEGLHGFIPSFDRIVDDVIERYSKVK--------------------------------- 182

Query: 190 RAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVSQV 249
             ENPAFSALPSFLFGQSLGGAV+LK+HLKQPRSW GAVLVAPMCKI+DDM+PPW V+QV
Sbjct: 183 --ENPAFSALPSFLFGQSLGGAVSLKVHLKQPRSWSGAVLVAPMCKIADDMVPPWAVAQV 242

Query: 250 LIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELE 309
           LIG SKFLPKYKLVPQKDLAE AFRDLKYRE+T YNVIAYK KPRLQTAVEML+TTQE+E
Sbjct: 243 LIGVSKFLPKYKLVPQKDLAEVAFRDLKYRELTAYNVIAYKDKPRLQTAVEMLKTTQEIE 302

Query: 310 RRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIV 341
           RRL+E+SLP+LILHGEADTVTDPSVSK LYEK+SS+D+K+ LY++A+HSLLEGEPDE+I+
Sbjct: 303 RRLKEISLPLLILHGEADTVTDPSVSKVLYEKASSSDKKIKLYKDAYHSLLEGEPDEVIL 342

BLAST of Cp4.1LG13g07730 vs. TAIR 10
Match: AT5G16120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 369.4 bits (947), Expect = 3.1e-102
Identity = 194/364 (53.30%), Postives = 244/364 (67.03%), Query Frame = 0

Query: 18  DDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFKV--------------------- 77
           ++EL++L + N+D+   RRR R + K IQL++DHILFK                      
Sbjct: 10  NEELRRLREVNIDEAPGRRRVRDSLKDIQLNLDHILFKTPENGIKTKESFEVNSRGVEIF 69

Query: 78  --FWF--------------------TVSFLGIARKFALLGYSVFAMDYPGFGLSEGLHGF 137
              W                     T  F GIAR+ AL GY VFAMDYPGFGLSEGLHG+
Sbjct: 70  SKSWLPEASKPRALVCFCHGYGDTCTFFFEGIARRLALSGYGVFAMDYPGFGLSEGLHGY 129

Query: 138 IPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFIYLIPRAENPAFS 197
           IP+FD +V DVIEHYS +K                                    NP FS
Sbjct: 130 IPSFDLLVQDVIEHYSNIKA-----------------------------------NPEFS 189

Query: 198 ALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVSQVLIGASKFL 257
           +LPSFLFGQS+GGAV+LKIHLKQP +W GAVL+APMCKI+DD++PP  + Q+LIG +  L
Sbjct: 190 SLPSFLFGQSMGGAVSLKIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVL 249

Query: 258 PKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELERRLREVSL 317
           PK+KLVPQKDLAEA FRD++ R+MT YN+I Y  KPRL+TAVEMLRTTQ++E++L+EVSL
Sbjct: 250 PKHKLVPQKDLAEAGFRDIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQEVSL 309

Query: 318 PILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIVQVLDDIIS 339
           PILILHGEADTVTDPSVS+ LYEK+ S D+K++LYE A+HSLLEGEPD++I++VL DIIS
Sbjct: 310 PILILHGEADTVTDPSVSRELYEKAKSPDKKIVLYENAYHSLLEGEPDDMILRVLSDIIS 338

BLAST of Cp4.1LG13g07730 vs. TAIR 10
Match: AT5G16120.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 369.4 bits (947), Expect = 3.1e-102
Identity = 194/364 (53.30%), Postives = 244/364 (67.03%), Query Frame = 0

Query: 18  DDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFKV--------------------- 77
           ++EL++L + N+D+   RRR R + K IQL++DHILFK                      
Sbjct: 28  NEELRRLREVNIDEAPGRRRVRDSLKDIQLNLDHILFKTPENGIKTKESFEVNSRGVEIF 87

Query: 78  --FWF--------------------TVSFLGIARKFALLGYSVFAMDYPGFGLSEGLHGF 137
              W                     T  F GIAR+ AL GY VFAMDYPGFGLSEGLHG+
Sbjct: 88  SKSWLPEASKPRALVCFCHGYGDTCTFFFEGIARRLALSGYGVFAMDYPGFGLSEGLHGY 147

Query: 138 IPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFIYLIPRAENPAFS 197
           IP+FD +V DVIEHYS +K                                    NP FS
Sbjct: 148 IPSFDLLVQDVIEHYSNIKA-----------------------------------NPEFS 207

Query: 198 ALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWGVSQVLIGASKFL 257
           +LPSFLFGQS+GGAV+LKIHLKQP +W GAVL+APMCKI+DD++PP  + Q+LIG +  L
Sbjct: 208 SLPSFLFGQSMGGAVSLKIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVL 267

Query: 258 PKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTTQELERRLREVSL 317
           PK+KLVPQKDLAEA FRD++ R+MT YN+I Y  KPRL+TAVEMLRTTQ++E++L+EVSL
Sbjct: 268 PKHKLVPQKDLAEAGFRDIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQEVSL 327

Query: 318 PILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPDEIIVQVLDDIIS 339
           PILILHGEADTVTDPSVS+ LYEK+ S D+K++LYE A+HSLLEGEPD++I++VL DIIS
Sbjct: 328 PILILHGEADTVTDPSVSRELYEKAKSPDKKIVLYENAYHSLLEGEPDDMILRVLSDIIS 356

BLAST of Cp4.1LG13g07730 vs. TAIR 10
Match: AT1G77420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.4e-72
Identity = 153/377 (40.58%), Postives = 215/377 (57.03%), Query Frame = 0

Query: 7   IIAPKKKLHGIDDELQKLLDANMDDVSARRRARAAFKGIQLSIDHILFKVF--------W 66
           + A +  + G+ DEL  +   N+D   ARR AR+AF  +QL +DH LFK          W
Sbjct: 37  VCAKRSPIDGVSDELNLIASQNLDQAPARRLARSAFVDLQLQLDHCLFKKAPSGIRTEEW 96

Query: 67  F------------------------------------TVSFLGIARKFALLGYSVFAMDY 126
           +                                    T  F GIA++ A  GY V+A+D+
Sbjct: 97  YERNSKGEDIFCKSWLPKSGDEIKAAVCFCHGYGSTCTFFFDGIAKQIAGFGYGVYAIDH 156

Query: 127 PGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKNLEQQNSDMHKFI 186
           PGFGLS+GLHG IP+FD + D+ IE ++K+KG                 E +N       
Sbjct: 157 PGFGLSDGLHGHIPSFDDLADNAIEQFTKMKGRS---------------ELRN------- 216

Query: 187 YLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPMCKISDDMIPPWG 246
                        LP FL GQS+GGAVALKIHLK+P++W G +LVAPMCKIS+D+ PP  
Sbjct: 217 -------------LPRFLLGQSMGGAVALKIHLKEPQAWDGLILVAPMCKISEDVKPPPL 276

Query: 247 VSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKPRLQTAVEMLRTT 306
           V + LI  S   PK KL P++DL++  FRDL  R++  Y+VI Y  + RL+TAVE+L  T
Sbjct: 277 VLKTLILMSTLFPKAKLFPKRDLSDFFFRDLSKRKLCEYDVICYDDQTRLKTAVELLNAT 336

Query: 307 QELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYEEAWHSLLEGEPD 340
           +++E ++ +VSLP+LILHG+ D VTDP+VSK L++ + S D+ L LY   +H +LEG+ D
Sbjct: 337 RDIEMQVDKVSLPLLILHGDTDKVTDPTVSKFLHKHAVSQDKTLKLYPGGYHCILEGDTD 378

BLAST of Cp4.1LG13g07730 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 214.9 bits (546), Expect = 9.9e-56
Identity = 109/270 (40.37%), Postives = 159/270 (58.89%), Query Frame = 0

Query: 71  ALLGYSVFAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDCKN 130
           A +G++ FA+D  G G S+G+  ++P+ D +VDD+I  ++ +K                 
Sbjct: 81  AQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIK----------------- 140

Query: 131 LEQQNSDMHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVAPM 190
                             +NP F  LP FLFG+S+GGA+ L I    P  + GAVLVAPM
Sbjct: 141 ------------------QNPKFQGLPRFLFGESMGGAICLLIQFADPLGFDGAVLVAPM 200

Query: 191 CKISDDMIPPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKHKP 250
           CKISD + P W V Q LI  S+FLP + +VP +DL E + +  + + +   N + Y  KP
Sbjct: 201 CKISDKVRPKWPVDQFLIMISRFLPTWAIVPTEDLLEKSIKVEEKKPIAKRNPMRYNEKP 260

Query: 251 RLQTAVEMLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLILYE 310
           RL T +E+LR T  L ++L++VS+P +I+HG AD VTDP VS+ LYE + S D+ L +Y+
Sbjct: 261 RLGTVMELLRVTDYLGKKLKDVSIPFIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYD 315

Query: 311 EAWHSLLEGEPDEIIVQVLDDIISWMDERC 341
              HS+L GEPD+ I  V  DI+SW+++RC
Sbjct: 321 GMMHSMLFGEPDDNIEIVRKDIVSWLNDRC 315

BLAST of Cp4.1LG13g07730 vs. TAIR 10
Match: AT2G47630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 213.8 bits (543), Expect = 2.2e-55
Identity = 116/271 (42.80%), Postives = 157/271 (57.93%), Query Frame = 0

Query: 69  KFALLGYSVFAMDYPGFGLSEGLHGFIPNFDRIVDDVIEHYSKVKGHPTIIWRDDVDFDC 128
           + A  GY+VF MDY G G S G   +I  F  +V+D  ++Y+ +               C
Sbjct: 58  RLASAGYAVFGMDYEGHGRSMGSRCYIKKFANVVNDCYDYYTSI---------------C 117

Query: 129 KNLEQQNSDMHKFIYLIPRAENPAFSALPSFLFGQSLGGAVALKIHLKQPRSWGGAVLVA 188
              E  +                       FL+G+S+GGAV L +H K P  W GA+LVA
Sbjct: 118 AQEEYMDKG--------------------RFLYGESMGGAVTLLLHKKDPLFWNGAILVA 177

Query: 189 PMCKISDDMIPPWGVSQVLIGASKFLPKYKLVPQKDLAEAAFRDLKYREMTTYNVIAYKH 248
           PMCKIS+ + P   V  +L    + +PK+K+VP KD+ +AAF+DL  RE    N + Y+ 
Sbjct: 178 PMCKISEKVKPHPIVINLLTRVEEIIPKWKIVPTKDVIDAAFKDLVKREEVRNNKLIYQD 237

Query: 249 KPRLQTAVEMLRTTQELERRLREVSLPILILHGEADTVTDPSVSKALYEKSSSTDRKLIL 308
           KPRL+TA+EMLRT+  LE  L E+++P  +LHGEADTVTDP VSKALYEK+S+ D+ L L
Sbjct: 238 KPRLKTALEMLRTSMNLEDTLHEITMPFFVLHGEADTVTDPEVSKALYEKASTRDKTLKL 293

Query: 309 YEEAWHSLLEGEPDEIIVQVLDDIISWMDER 340
           Y   WH+L  GEPD  +  V  DII+W+D R
Sbjct: 298 YPGMWHALTSGEPDCNVDLVFADIINWLDLR 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9426.3e-3131.10Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1[more]
O356781.1e-1425.75Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1[more]
Q996855.3e-1424.64Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2[more]
Q8R4319.1e-1424.73Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1[more]
P283219.8e-0823.44Monoglyceride lipase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX... [more]
Match NameE-valueIdentityDescription
KAG7016061.11.47e-19982.27Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6578497.11.57e-18577.30Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038890284.12.17e-16765.96caffeoylshikimate esterase isoform X1 [Benincasa hispida][more]
XP_038890285.14.89e-16765.78caffeoylshikimate esterase isoform X2 [Benincasa hispida][more]
XP_008459473.12.46e-16465.00PREDICTED: caffeoylshikimate esterase isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A1S3CBH41.19e-16465.00caffeoylshikimate esterase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498599 PE... [more]
A0A6J1JHR73.65e-16464.49caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111487110 PE=4... [more]
A0A1S4E2J53.70e-16464.99caffeoylshikimate esterase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498599 PE... [more]
A0A6J1GRR02.03e-16364.40caffeoylshikimate esterase OS=Cucurbita moschata OX=3662 GN=LOC111456899 PE=4 SV... [more]
A0A0A0KSP33.51e-16264.80Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G644590... [more]
Match NameE-valueIdentityDescription
AT5G16120.13.1e-10253.30alpha/beta-Hydrolases superfamily protein [more]
AT5G16120.23.1e-10253.30alpha/beta-Hydrolases superfamily protein [more]
AT1G77420.13.4e-7240.58alpha/beta-Hydrolases superfamily protein [more]
AT1G11090.19.9e-5640.37alpha/beta-Hydrolases superfamily protein [more]
AT2G47630.12.2e-5542.80alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 65..113
e-value: 9.3E-10
score: 38.2
coord: 128..321
e-value: 1.3E-37
score: 129.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 56..341
e-value: 3.0E-46
score: 159.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 63..340
NoneNo IPR availablePANTHERPTHR11614:SF146ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 61..340
coord: 15..58
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 61..340
coord: 15..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g07730.1Cp4.1LG13g07730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016298 lipase activity