Cp4.1LG13g06820 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g06820
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMannose-6-phosphate isomerase
LocationCp4.1LG13: 4387505 .. 4391856 (+)
RNA-Seq ExpressionCp4.1LG13g06820
SyntenyCp4.1LG13g06820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCCAAACAAACTGGATTCAGAGGAAACTCGGACCCGACCCGACCCGAGTGGGATCAACGGCATTCGGAAGGGAGAGGAGGACTGAAAGGCTGACTAGCTCCAGGTATGCTAACGGCTCCCTCTCTCCATCTTTCTCAGTTATAAGATATAACGAACACGGAATCCGAATCTCCTAAAATCCCAAGAGAATCTCGATTTCTTCTTCAAGGGCTTTGGTATTTCATTTCTTGGTTTTTGGGTTTTCATTTGTGACTTACCTCCATTTTTGTGGCTCTCGCTGATGAGCAATGAACGAGGAAGATGATAACAGATTGAAGAAGAGGAAGAAGAAGAGACTGTTAGGCGCCGACAGGAGGAAGCGCCTCCTTCGCCTAAAATGCTATGTCCAAAACTATGACTGGGGGGTTATTGGGTGTGATTCCCAAGTCGCGAGGCTGTTTTCGCTTAATTCAGGTTCCGGCACTCCCGATCCGAGGGAATGTTACGCCGAGTTCTGGATTGGGACGCATAAATCCGGCCCCTCATTTGTTGTTTTTGGACGGGATTATAATAATGCCGCCGCTGCGCTTGGATCCAACGCCGTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTGGGTGATAAGGTTGCGAGGAAATGGGGAGGCGATCTTCCCTTTCTCTTCAAGGTCCGCTTTCTTTTCCATTAGATCCTTGGGGAATGTGTTTTGTCGATGTACTTGATTTCTTTCAAGATTTCAGAAATGCACACAAATTTCGGGTTATAATTATGCTCTAATTGTGTCGGTGTGGGAGATAATCAGGTTAAATAATCATAATGCTTCATAAACCAAAATTATGTGCTCCAGCTCTGTGAATTGTCTGTTCTTAGTTTCTGGAACCTCAATTACCGTTCTTATTTTCCTTGATGTCAATGTTTTGAGTTCAGTTGGACTGCAACTTGATGATTTCTTATTGGGAGCATAGGTGCTGTCTGTAGAGAAGGCTCTATCCATTCAGGCTCATCCGGATAAAGAGTTGGCGAGAGCTTTGCACGAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGGTTTGAAGCCCTTTGTGGGTTCATCAGCTCTAAGGTACGACAACATAGTAGCAAAGGGAAGTTCTTCTGGGGTTTTTGAAATTCTTCTTATCCTTGTTTTGTCTGTTGTTTAAATGAAAACATCTTAATGATTCTTACTCTGTCCTGTTACACAAATTGCATTTAGAAAGATGAAAGAAACTCAAATCAAAAAGTTGATTCAGCTTTTTGGTTCATTGTGTATGTATCTCCAGATGAGTGAATTCAGGAAAATAAACGAAGAATTGAAGTAATTGATAATGAATGAAGTTTCTTTATAAATACATGTAATTTGATATTACTTATTTCATTATTTGTCAGGAGCTCAAGGGGGTGCTGAGTAGCGTTCCTGAGATAGTAGAGTTGGTCCAATGTGGAGATGCAGAGAAGTTCTCACACGATAGTGGGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTATATGTAAATTAAAAAGGCGTCTAAGTTTCGAGAAAAAGGTAACCGAACTTGGTATTTTTCATCATAGAAGTTGTTATGTTTTAAATTTCAAGGCTTGCTACTTCCCTATAACTTGCGGCATGGATATTAAAACATTAAACACATAGAAGGTTTCCATCTGTATACTGTATGCATATGCAACTTATTGGTGAAAGAAAGCTGCTAATGTGAATTTAAAGGTGGCTGGTGAATACTTTTACATATGATTGTATAAGCAAAGTTGGTAACTTATATTTTGTAGGCGGTGATGCAATGATTTAAATTTTCCCAAATATTCTTATGTAGTAGCAATCTTTTAGTGACCATTAAACATAGATTGTATGACAACCTTAGCTTGCAGATTAACTATTGGCTACAATTGTTTCTGAAAATATTAGTTTTCACTGCCAATTTGTCAATAATCATAGACTGTATGACAGCTTTGGTTTGCAGCCGTAGGGCTCTTCTGCTAAGTTTTGCATCTTCTACGAGAATGTGAATTTATATGTTTTAGCATGGTAAGGATAATATAATATAGGGCACATCTAAAACTTGAACAGCCTAGGATCGATCAACTCCTCATGCTTTCCGATAGCCTGTCCAGCAAATGTTTGGCTTTTAGCATGACAGTTTTAGGTTAGAATGTGTTACTGAGTCTATTAAATGGTCGCCGTTTGGATTATTCTAGAATCCAGACCATGTAGAATCAATTAATCAGCTTATGTTAGTTGTTTGGGGAACAATAAATATCGTAAACCATTGATGGGATTATTGATGTGATGACCTAAACAAGCCAATAGAATTTGTGAGATCCCACATCGGTTGAAGAGGGGAACGAAACATATTTATAAGGGTGTCGAAATCTCTTCCTAGCAGACGCGTTTTAAAAACCTTGAGGGAAAGGCCAAAGAGGACAATATCTGCTATCGATGGACTTGGGCTGTTACGGAATTGATGCGGTGGAGATGAGGTATTGTAGTAGGGTAAAAGCTAATGACAAACAAAGGCCATTTGCAACCGCTCAATTGATTATGTTTTCATCACTGGGCACAAGCCAACCGAATTGATGAGTTAAGGAATCGTGGTACATCTAGGGCATGCTAGCGTTTCCCGTGTTAGCCATCTTGTGCAGTTAAATCTGAGTAGAAAGTAGGATGTGTGAATCTCTTTAAGCCTTTTGCTAATAGATAGTGTCAGCTCAGGAGCAAGATCACATTCTGTTTGAAGGTATTTCAGTTAACATAAATCATCATTAGCAAGTTCTATGATTGAAAGGATACATTTCATAATAAAGCTAAGGCTGTTCTTTCTCACTTCATTGATCTCTGCAGAGTTTTATTCGTTTTCTTTCTTAGTCTTTTTTTGCGACTTCTCATTGTATTACACTCTCTTTGTTTTTTCATCAGAAGAGACAATTGTCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATATCCAGCTGATGTTGGTATACTAGCAACATTTTTTCTCAACTATGTCAAGTTAAAACCGGGCGAAGCGTTATATGTTGGTCCAAACGAACCGCATGCGTATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAACGTTGTTCGGGCTGGCCTTACCTCGAAGAAAAGAGACGTTCAAGCCCTTCTTTCAATGCTCAACTACAAACAGGTAATCACAGAATTTATGTTAAGCTGGTGTCTTATAATGCTGCAATTCTAACGTGTTAAAATGATTATCGCCTTTTGGTTCTTGTTTTCGATGTCCTCTTTCTCGAAGTCCCGCCGCAGGCTTACCTGTTAAAGTTAATTAATCTGCATTTTGGATTTTGACCTGTAGTCTTACATGCACAAATATGCAACCACTATTCACAACAGGGTATTCATATTGGTCTGAGCTCACCTAGATTTTCATATGAGTAGTTTATCTTCCCCCCCCCCNGTGAAAAACGCCTCAGCCGTACAATTTTGAACGACTTGATTCATATCGATCTTCGTTTTCACCTCCTCTAATCTGCCCTGTCCTACGCCTTAGAGTTTTACTGAGAAGATTGTTCATAATCATTTCATTCAACATACAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCGTTGAACGAATACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTTCCTCAAGCTGCATCTGTGTCTTTCCCTTCTGTCCCAGGACCATCTCTCTTTCTGGTGATATCTGGAAAAGGGACAATTATCACAGGCTATTCTGAAGAAAGTACATTAGAAGAGGGTGAAGTTCTGTTCGTTCCAGCATACATGGAGGTCAGTATAACATCTGGAGCTACAGAATTACATATGTACAGAGCTGGCGTCAACAACAGATTCTTTGGGGACTTGTGATTTATACAAGTACAAATGAATAAATCAAGAATATACAGGAAGAAAACAGGTTATAAGAAGAATTGTTGCATTGATTGTTCTTTGTGATCTGCTCATTTTTATTGATTAAATTGCATCAAAGTTGTGCATATACATTCATTAGAAGTATAAATAGGGTATTGAGTTATATTCAAGTTCTTCATGATTGCAGATTGCTTTATTTATTTCTGTCCTGTCAGTTTATCAGCTGTCAGAAGTGGATATTCAAAGTTCTTTGTTTGGGAATATTAAATCAAATGCTCCCTTCCCAATCCTCATTTCAGGATA

mRNA sequence

TGTCCAAACAAACTGGATTCAGAGGAAACTCGGACCCGACCCGACCCGAGTGGGATCAACGGCATTCGGAAGGGAGAGGAGGACTGAAAGGCTGACTAGCTCCAGGTATGCTAACGGCTCCCTCTCTCCATCTTTCTCAGTTATAAGATATAACGAACACGGAATCCGAATCTCCTAAAATCCCAAGAGAATCTCGATTTCTTCTTCAAGGGCTTTGGTATTTCATTTCTTGGTTTTTGGGTTTTCATTTGTGACTTACCTCCATTTTTGTGGCTCTCGCTGATGAGCAATGAACGAGGAAGATGATAACAGATTGAAGAAGAGGAAGAAGAAGAGACTGTTAGGCGCCGACAGGAGGAAGCGCCTCCTTCGCCTAAAATGCTATGTCCAAAACTATGACTGGGGGGTTATTGGGTGTGATTCCCAAGTCGCGAGGCTGTTTTCGCTTAATTCAGGTTCCGGCACTCCCGATCCGAGGGAATGTTACGCCGAGTTCTGGATTGGGACGCATAAATCCGGCCCCTCATTTGTTGTTTTTGGACGGGATTATAATAATGCCGCCGCTGCGCTTGGATCCAACGCCGTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTGGGTGATAAGGTTGCGAGGAAATGGGGAGGCGATCTTCCCTTTCTCTTCAAGGTGCTGTCTGTAGAGAAGGCTCTATCCATTCAGGCTCATCCGGATAAAGAGTTGGCGAGAGCTTTGCACGAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGGTTTGAAGCCCTTTGTGGGTTCATCAGCTCTAAGGAGCTCAAGGGGGTGCTGAGTAGCGTTCCTGAGATAGTAGAGTTGGTCCAATGTGGAGATGCAGAGAAGTTCTCACACGATAGTGGGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTATATGTAAATTAAAAAGGCGTCTAAGTTTCGAGAAAAAGAAGAGACAATTGTCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATATCCAGCTGATGTTGGTATACTAGCAACATTTTTTCTCAACTATGTCAAGTTAAAACCGGGCGAAGCGTTATATGTTGGTCCAAACGAACCGCATGCGTATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAACGTTGTTCGGGCTGGCCTTACCTCGAAGAAAAGAGACGTTCAAGCCCTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCGTTGAACGAATACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTTCCTCAAGCTGCATCTGTGTCTTTCCCTTCTGTCCCAGGACCATCTCTCTTTCTGGTGATATCTGGAAAAGGGACAATTATCACAGGCTATTCTGAAGAAAGTACATTAGAAGAGGGTGAAGTTCTGTTCGTTCCAGCATACATGGAGGTCAGTATAACATCTGGAGCTACAGAATTACATATGTACAGAGCTGGCGTCAACAACAGATTCTTTGGGGACTTGTGATTTATACAAGTACAAATGAATAAATCAAGAATATACAGGAAGAAAACAGGTTATAAGAAGAATTGTTGCATTGATTGTTCTTTGTGATCTGCTCATTTTTATTGATTAAATTGCATCAAAGTTGTGCATATACATTCATTAGAAGTATAAATAGGGTATTGAGTTATATTCAAGTTCTTCATGATTGCAGATTGCTTTATTTATTTCTGTCCTGTCAGTTTATCAGCTGTCAGAAGTGGATATTCAAAGTTCTTTGTTTGGGAATATTAAATCAAATGCTCCCTTCCCAATCCTCATTTCAGGATA

Coding sequence (CDS)

ATGAACGAGGAAGATGATAACAGATTGAAGAAGAGGAAGAAGAAGAGACTGTTAGGCGCCGACAGGAGGAAGCGCCTCCTTCGCCTAAAATGCTATGTCCAAAACTATGACTGGGGGGTTATTGGGTGTGATTCCCAAGTCGCGAGGCTGTTTTCGCTTAATTCAGGTTCCGGCACTCCCGATCCGAGGGAATGTTACGCCGAGTTCTGGATTGGGACGCATAAATCCGGCCCCTCATTTGTTGTTTTTGGACGGGATTATAATAATGCCGCCGCTGCGCTTGGATCCAACGCCGTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTGGGTGATAAGGTTGCGAGGAAATGGGGAGGCGATCTTCCCTTTCTCTTCAAGGTGCTGTCTGTAGAGAAGGCTCTATCCATTCAGGCTCATCCGGATAAAGAGTTGGCGAGAGCTTTGCACGAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGGTTTGAAGCCCTTTGTGGGTTCATCAGCTCTAAGGAGCTCAAGGGGGTGCTGAGTAGCGTTCCTGAGATAGTAGAGTTGGTCCAATGTGGAGATGCAGAGAAGTTCTCACACGATAGTGGGCAAGATGGGAAGGAGAAAGTAAAACAACTTTTTGAGTCCATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTATATGTAAATTAAAAAGGCGTCTAAGTTTCGAGAAAAAGAAGAGACAATTGTCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATATCCAGCTGATGTTGGTATACTAGCAACATTTTTTCTCAACTATGTCAAGTTAAAACCGGGCGAAGCGTTATATGTTGGTCCAAACGAACCGCATGCGTATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAACGTTGTTCGGGCTGGCCTTACCTCGAAGAAAAGAGACGTTCAAGCCCTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCGTTGAACGAATACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTTCCTCAAGCTGCATCTGTGTCTTTCCCTTCTGTCCCAGGACCATCTCTCTTTCTGGTGATATCTGGAAAAGGGACAATTATCACAGGCTATTCTGAAGAAAGTACATTAGAAGAGGGTGAAGTTCTGTTCGTTCCAGCATACATGGAGGTCAGTATAACATCTGGAGCTACAGAATTACATATGTACAGAGCTGGCGTCAACAACAGATTCTTTGGGGACTTGTGA

Protein sequence

MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTPDPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVLFVPAYMEVSITSGATELHMYRAGVNNRFFGDL
Homology
BLAST of Cp4.1LG13g06820 vs. ExPASy Swiss-Prot
Match: Q9FZH5 (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 3.9e-129
Identity = 230/429 (53.61%), Postives = 304/429 (70.86%), Query Frame = 0

Query: 24  KRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTPDPRECYAEFWIGTHKSGPSFVVF 83
           + + RL+C+V+NY+WG +G +S VARL   N+G    D    YAEFW+GTH+SGPS V F
Sbjct: 20  EEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQRV-DSEIPYAEFWMGTHESGPSHVEF 79

Query: 84  GRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAH 143
           G  +  +   +    V+LK W+  +P  +LG KV  KWG DLPFLFKVLSV KALSIQAH
Sbjct: 80  GSGHGVSDKCM----VTLKSWVLDNPN-LLGSKVVDKWGCDLPFLFKVLSVTKALSIQAH 139

Query: 144 PDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQ 203
           P+K LA  LH   P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV 
Sbjct: 140 PNKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVG 199

Query: 204 CGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSFEKKKRQLSA 263
              A++  +    D  E++K +   IF+Q+MS++    ++ + ++K RL  E K R+LS 
Sbjct: 200 SKAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSE 259

Query: 264 KEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAYISGECIECMATSDNV 323
           KE+L+L LE QY  D+G+++ FF NYVKL PGEALY+  NEPHAYISG+C+ECMA SDNV
Sbjct: 260 KEKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNV 319

Query: 324 VRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAAS 383
           VRAGLT K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP+  S
Sbjct: 320 VRAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKS 379

Query: 384 VSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVLFVPAYMEVSITSGATELHMYRAG 443
             FP+VPGPS++LVI GKG + TG S +  +  G+VLFVPA +E+ +T  +  + +YRAG
Sbjct: 380 TVFPAVPGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAG 439

Query: 444 VNNRFFGDL 453
           V++RFF  L
Sbjct: 440 VSSRFFQTL 441

BLAST of Cp4.1LG13g06820 vs. ExPASy Swiss-Prot
Match: Q9M884 (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 6.6e-129
Identity = 238/429 (55.48%), Postives = 313/429 (72.96%), Query Frame = 0

Query: 20  ADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTPDPRECYAEFWIGTHKSGPS 79
           ADRR RL RL+C V++YDWG IG DS V R+++ NS     DP   YAE W+GTH+SGPS
Sbjct: 15  ADRR-RLRRLRCSVKDYDWGKIGSDSLVYRVYAANSDYEI-DPTRPYAELWMGTHESGPS 74

Query: 80  FVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALS 139
           ++           A GSN V+L+ WI+ +P + LG++V  KWG DLPFLFKVLSV + LS
Sbjct: 75  YL---------EDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLS 134

Query: 140 IQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIV 199
           IQAHPDK+LA+ +H+A P++YKDDNHKPEMALA T FEALCGFI  +ELK V+ ++PEI 
Sbjct: 135 IQAHPDKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIE 194

Query: 200 ELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSFEKKKR 259
           ELV   +A +    +  D +EKVK +  +IF+ +MS++    ++ + KLKRRL  E ++R
Sbjct: 195 ELVGSEEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQER 254

Query: 260 QLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAYISGECIECMAT 319
           QL+ KE+L+L+LE QYP D+G+++ FF NYVKL PGEALY+G NEPHAY+ GEC+E MAT
Sbjct: 255 QLTDKERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMAT 314

Query: 320 SDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILP 379
           SDNVVRAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP
Sbjct: 315 SDNVVRAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLP 374

Query: 380 QAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVLFVPAYMEVSITSGATELHM 439
             AS  FPSVPGPSL LV+ G+G + T  S +  +  G+VLFVPA  E+ + S +++L +
Sbjct: 375 SGASTVFPSVPGPSLLLVLQGEGRMSTEASADG-ISMGDVLFVPADTEIHLRS-SSDLKL 428

Query: 440 YRAGVNNRF 449
           YRAG+N+RF
Sbjct: 435 YRAGINSRF 428

BLAST of Cp4.1LG13g06820 vs. ExPASy Swiss-Prot
Match: Q54PA0 (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=mpi PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 5.5e-75
Identity = 173/449 (38.53%), Postives = 253/449 (56.35%), Query Frame = 0

Query: 21  DRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTPDPREC--YAEFWIGTHKSGP 80
           +    ++ LKC  QNY+WG  G +S VA+L    +   +   +E   YAE W+G H S P
Sbjct: 16  NNNNNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAP 75

Query: 81  SFVVFGRDYNNAAAALGSNAVSLKDWI-------SVDPGAVLGDKVARKWGGDLPFLFKV 140
           S V    +Y N    L     +++  I       S    ++ G+ V +++G D PFLFKV
Sbjct: 76  SKV----EYKNKELKLREYIDTVQKEINEKSSTSSSSSSSIRGEIVEKRFGNDFPFLFKV 135

Query: 141 LSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGV 200
           LS+  ALSIQAHPD +LA+ L +  P+IYKD  HKPE+A+A T FEALC F    E++  
Sbjct: 136 LSIRTALSIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPLSEIQSF 195

Query: 201 LSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRR 260
           + ++PE           K S  +     +  K+  +SI + ++ ++  ++   + +L  R
Sbjct: 196 IDTIPEF----------KNSLPNNLIKLDDCKEYLKSIVTSLLKADGLLISNNLKELNNR 255

Query: 261 LSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAYISG 320
           L+ EK++ +    ++L+L+L  QYP DVG+   + LNY+ LKPGEAL++G  EPHAYISG
Sbjct: 256 LN-EKREEERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEPHAYISG 315

Query: 321 ECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL-----QGVSLNEYTRKYAP 380
           +C+ECMA SDNVVRAGLT K +DV  L  ML Y+ G P+++       +SL  Y R Y P
Sbjct: 316 DCVECMAPSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLVVPQQRPDLSLTNY-RCYQP 375

Query: 381 PFDEFEVDRCILPQAA-SVSFPSVPGPSLFLVISGKGTIITGYSEESTLEE---GEVLFV 440
           P DEF+V+   L     +++  S  GPS+ LV SG    I   S +STL     G +LFV
Sbjct: 376 PVDEFQVEYYHLDDCCKNINVYSSKGPSIVLVYSG-NLSIENKSNQSTLNNLHTGSILFV 435

Query: 441 PAYMEVSI--TSGATELHMYRAGVNNRFF 450
           PA  +     +  +  + +Y A V+NR F
Sbjct: 436 PANTDYQFIQSDSSIPVSIYVASVSNRIF 447

BLAST of Cp4.1LG13g06820 vs. ExPASy Swiss-Prot
Match: Q9HFU4 (Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=MAN1 PE=3 SV=2)

HSP 1 Score: 275.0 bits (702), Expect = 1.5e-72
Identity = 177/441 (40.14%), Postives = 238/441 (53.97%), Query Frame = 0

Query: 33  VQNYDWGVIGCDSQVARLFSLNSGSGTPDPRECYAEFWIGTHKSGPSFVVFGRDYNNAAA 92
           + +YDWG  G  S  A+L + +    + D  + YAE W+GTH + PS +           
Sbjct: 12  INSYDWGKKGSASLAAQLATTSIPDFSIDEDKAYAELWMGTHPNNPSRL----------- 71

Query: 93  ALGSNAVSLKDWISVDPGAVLGDKVARKW----GGDLPFLFKVLSVEKALSIQAHPDKEL 152
              S+   L + +   P  ++G  V+ K+     G LPFLFKVLS+  ALSIQAHPDK L
Sbjct: 72  ---SDNTLLSEHLKSHP-ELIGSSVSSKFEDCKDGSLPFLFKVLSIGTALSIQAHPDKPL 131

Query: 153 ARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQCGDAE 212
           A+ L + +P +YKD NHKPEMA+ALT F A   F+    L   L +VPE+ E V     E
Sbjct: 132 AKKLFDEKPDVYKDPNHKPEMAIALTPFLAFLNFLPLSVLLLHLLTVPELQEFVDSSLTE 191

Query: 213 KFSHDSG----------------QDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRL 272
             +   G                     + K + + IF+ +MS++K +V EAI KL +R 
Sbjct: 192 SLASSLGLPTSQPPDTSLFKPTESPATAEQKDILKQIFAALMSADKKLVEEAISKLIKRY 251

Query: 273 SFEKKKRQLSAKEQ----LILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAY 332
              + KR +   E+    L LRL  QYP DVG+L  F LN V+LK GEA ++G NEPHAY
Sbjct: 252 ---QAKRDIKENEKSLVDLALRLNDQYPGDVGVLCVFLLNVVELKRGEAAFLGANEPHAY 311

Query: 333 ISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYK--QGFPEILQGVSL---NEYTRK 392
           I G+ IECMATSDNVVRAGLT K RDV  L+SML Y+   G  ++LQ       ++ T+ 
Sbjct: 312 IEGDIIECMATSDNVVRAGLTPKLRDVDTLVSMLTYEAAPGNKQLLQPTPFQKGDDTTKL 371

Query: 393 YAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVLFVP 445
           Y PP  EF V R  L +    S   V GPSL ++  G+G +  G ++ S    G+V+FV 
Sbjct: 372 YDPPIAEFSVLRTELSKGMKTSHRPVEGPSLCVITEGEGVVRNG-NDRSEFVRGDVIFVG 431

BLAST of Cp4.1LG13g06820 vs. ExPASy Swiss-Prot
Match: Q9GP38 (Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN=PMIH PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.4e-72
Identity = 180/457 (39.39%), Postives = 251/457 (54.92%), Query Frame = 0

Query: 26  LLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTPDPREC---YAEFWIGTHKSGPSFVV 85
           +LRLKC  Q YDWG +G +S V   + L   S  PD       YAE W+GTH SG S + 
Sbjct: 1   MLRLKCASQRYDWGKVGVNSIV---YKLQVASENPDISHVDLPYAELWMGTHPSGSSCL- 60

Query: 86  FGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQA 145
               ++N    L ++       I  +P A LG      +G  LPFLFKVLSV KALSIQA
Sbjct: 61  ----WDNPHVTLAAH-------IQENP-ASLGKPSLIYFGRRLPFLFKVLSVAKALSIQA 120

Query: 146 HPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVEL- 205
           HPDK++A  LH  QP +YKD +HKPEMA+ALT FEAL GF    ++   + + PE+ EL 
Sbjct: 121 HPDKKMAVRLHAEQPDLYKDGSHKPEMAIALTDFEALLGFRPLNQILAFIQAFPELAELT 180

Query: 206 ------------VQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLK 265
                       VQ    ++   +  +   EKV+   +S+ ++  + +K +    +    
Sbjct: 181 TLELPSPEEKRDVQPPSIKQLYSNLMRSSPEKVESTIKSLLNRFTTGSKSVCDPPL---- 240

Query: 266 RRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAYI 325
             L  + ++  + A   L LRL   +P DVG L+ FFLNY++LK GEA+++  N PHAY+
Sbjct: 241 SILGVDLQEEDVQALVDLFLRLTQAFPGDVGCLSIFFLNYIRLKSGEAIFLKANTPHAYL 300

Query: 326 SGECIECMATSDNVVRAGLTSKKRDVQALLSMLNY-------KQGFPEIL---QGVSLNE 385
           SG+C+ECMA SDNVVRAGLT K +DV+ LL ML+Y       + G  + +   +G+S+  
Sbjct: 301 SGDCVECMANSDNVVRAGLTPKFKDVERLLEMLDYTPLTDSLRLGATQPIPTPEGISM-- 360

Query: 386 YTRKYAPPFDEFEVDRCIL-PQAASVSFPSVPGPSLFLVISGKGTII---TG--YSEEST 445
             + + PP  EF VD      ++   S PSVP  S+ L + G+GTI    TG   S+E+ 
Sbjct: 361 --KSFIPPVSEFAVDVIQFDAESRGFSLPSVPTASILLFLHGQGTITCPSTGEACSQETK 420

Query: 446 LEEGEVLFVPAYMEVSITSGAT---ELHMYRAGVNNR 448
              G V FVPA M V++ S       LH +RA VN +
Sbjct: 421 FGPGFVYFVPADMIVNLVSKEDRKGSLHAFRAYVNRK 433

BLAST of Cp4.1LG13g06820 vs. NCBI nr
Match: XP_023549674.1 (mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVSITSGATELHMYRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452

BLAST of Cp4.1LG13g06820 vs. NCBI nr
Match: KAG6579210.1 (Mannose-6-phosphate isomerase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016723.1 Mannose-6-phosphate isomerase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 889 bits (2298), Expect = 0.0
Identity = 445/452 (98.45%), Postives = 450/452 (99.56%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGC+SQVARLFSLNSGSGTP
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIY+DDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQCGD EKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILA FFLNYVKLKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTL+EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVSITSGATELH+YRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of Cp4.1LG13g06820 vs. NCBI nr
Match: XP_022938390.1 (mannose-6-phosphate isomerase 1-like [Cucurbita moschata])

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 444/452 (98.23%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGC+SQVARLFSLNSGSGTP
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIY+DDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQCGD EKFSHDSGQDGKEKVKQLFESIFSQIMSSNK I
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKAI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILA FFLNYVKLKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTL+EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVSITSGATELH+YRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of Cp4.1LG13g06820 vs. NCBI nr
Match: XP_022994025.1 (mannose-6-phosphate isomerase 1-like [Cucurbita maxima])

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 443/451 (98.23%), Postives = 448/451 (99.33%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGC+SQVARLFSLNSGSGTP
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGG+LPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGELPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VR+AICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILA FFLNYVKLKPGEALYV
Sbjct: 241 VRDAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSF SVPGPSLFLVISGKGTIITGYSEESTL+EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFASVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGD 451
           FVPAYMEVSITSGATEL +YRAGVNNRFFGD
Sbjct: 421 FVPAYMEVSITSGATELRLYRAGVNNRFFGD 451

BLAST of Cp4.1LG13g06820 vs. NCBI nr
Match: XP_038875726.1 (mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875727.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875728.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875729.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida])

HSP 1 Score: 820 bits (2119), Expect = 1.90e-298
Identity = 414/452 (91.59%), Postives = 431/452 (95.35%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           M+EEDDN+LKK +KKRL GADRRKRLLRLKC VQNYDWGVIGC+SQVARLF LNSG  + 
Sbjct: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRD NNA AA GS  +SLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRD-NNAGAAFGSKPLSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC DAEKFSH+S QDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREAICKLKRRLS EKKKRQLSAKEQLILRLEGQYPADVGILA FFLNYV+LKPGEALYV
Sbjct: 241 VREAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTI+TGYSEE T +EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVSIT+ A ELHMYRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITAAAIELHMYRAGVNNRFFGDL 451

BLAST of Cp4.1LG13g06820 vs. ExPASy TrEMBL
Match: A0A6J1FJN3 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3 SV=1)

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 444/452 (98.23%), Postives = 449/452 (99.34%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGC+SQVARLFSLNSGSGTP
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIY+DDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQCGD EKFSHDSGQDGKEKVKQLFESIFSQIMSSNK I
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKAI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILA FFLNYVKLKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTL+EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVSITSGATELH+YRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of Cp4.1LG13g06820 vs. ExPASy TrEMBL
Match: A0A6J1K047 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 SV=1)

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 443/451 (98.23%), Postives = 448/451 (99.33%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGC+SQVARLFSLNSGSGTP
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGG+LPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGELPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VR+AICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILA FFLNYVKLKPGEALYV
Sbjct: 241 VRDAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSF SVPGPSLFLVISGKGTIITGYSEESTL+EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFASVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGD 451
           FVPAYMEVSITSGATEL +YRAGVNNRFFGD
Sbjct: 421 FVPAYMEVSITSGATELRLYRAGVNNRFFGD 451

BLAST of Cp4.1LG13g06820 vs. ExPASy TrEMBL
Match: A0A6J1E4R0 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3 SV=1)

HSP 1 Score: 811 bits (2095), Expect = 4.01e-295
Identity = 408/452 (90.27%), Postives = 427/452 (94.47%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           M EEDD RLKKRKKKRL+GADRRKRL+RLKC VQNYDWGV+GC+SQVAR+F LNSGS + 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFV FGRDYN  AAA+GS  +SLKDWI+VDPGAVLGDKVARK
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYN--AAAIGSKPLSLKDWITVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQ  DAEKFSHDS Q+GKEKVK LFESIFSQIMSSNK +
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAM 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREAICKLKRRLS EKKKRQLSAKEQL+LRLE QYPADVGILA FFLNYVKLKPGEALY+
Sbjct: 241 VREAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYL 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL++SGKGTI+TGYSEE T +EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEEITFQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVS+T+ ATELHMYRAGVNNRFF DL
Sbjct: 421 FVPAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of Cp4.1LG13g06820 vs. ExPASy TrEMBL
Match: A0A6J1I443 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 SV=1)

HSP 1 Score: 810 bits (2093), Expect = 8.09e-295
Identity = 407/452 (90.04%), Postives = 426/452 (94.25%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           M EEDD RLKKRKKKRL+GADRR+RLLRLKC VQNYDWGV+GC+SQVAR+F LNSGS + 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFV FGRDYN  AAA+GS  +SLKDWI+VDPGAVLGDKVARK
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYN--AAAIGSKPLSLKDWITVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELKGVLSSVPEIVELVQ  DAEKFSHDS Q+GKEKVK LFESIF QIMSSNK +
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAM 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREAICKLKRRLS EKKKRQLS KEQL+LRLE QYPADVGILA FFLNYVKLKPGEALY+
Sbjct: 241 VREAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYL 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL++SGKGTI+TGYSEEST +EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVS+T+ ATELHMYRAGVNNRFF DL
Sbjct: 421 FVPAYMEVSVTAAATELHMYRAGVNNRFFRDL 450

BLAST of Cp4.1LG13g06820 vs. ExPASy TrEMBL
Match: A0A0A0KL16 (Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV=1)

HSP 1 Score: 800 bits (2065), Expect = 1.55e-290
Identity = 405/452 (89.60%), Postives = 425/452 (94.03%), Query Frame = 0

Query: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60
           M+EEDD +L+K +KKRL GADRRKRLLRLKC VQNYDWGVIGC+SQVARLF LNSG    
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120
           DP ECYAEFWIGTHKSGPSFVV+GRD NNA AA GS  +SLKD ISVDP AVLGDKVARK
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRD-NNAVAAFGSKPLSLKDCISVDPVAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L+EAQPSIYKDDNHKPEMALALT FEALC
Sbjct: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALC 180

Query: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC DAEKFSHDS QDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300
           VREA+CKLKRRLS EKKKRQLSAKEQLILRLE QYPADVGILA +FLNYV+LKPGEALYV
Sbjct: 241 VREAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTIITGYSEE+TL+EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVL 420

Query: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452
           FVPAYMEVSIT+ A ELHMYRAG+NNRFF DL
Sbjct: 421 FVPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of Cp4.1LG13g06820 vs. TAIR 10
Match: AT1G67070.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 463.0 bits (1190), Expect = 2.8e-130
Identity = 230/429 (53.61%), Postives = 304/429 (70.86%), Query Frame = 0

Query: 24  KRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTPDPRECYAEFWIGTHKSGPSFVVF 83
           + + RL+C+V+NY+WG +G +S VARL   N+G    D    YAEFW+GTH+SGPS V F
Sbjct: 20  EEIQRLRCFVKNYEWGKLGPESLVARLQEANTGQRV-DSEIPYAEFWMGTHESGPSHVEF 79

Query: 84  GRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALSIQAH 143
           G  +  +   +    V+LK W+  +P  +LG KV  KWG DLPFLFKVLSV KALSIQAH
Sbjct: 80  GSGHGVSDKCM----VTLKSWVLDNPN-LLGSKVVDKWGCDLPFLFKVLSVTKALSIQAH 139

Query: 144 PDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIVELVQ 203
           P+K LA  LH   P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV 
Sbjct: 140 PNKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVG 199

Query: 204 CGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSFEKKKRQLSA 263
              A++  +    D  E++K +   IF+Q+MS++    ++ + ++K RL  E K R+LS 
Sbjct: 200 SKAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSE 259

Query: 264 KEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAYISGECIECMATSDNV 323
           KE+L+L LE QY  D+G+++ FF NYVKL PGEALY+  NEPHAYISG+C+ECMA SDNV
Sbjct: 260 KEKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNV 319

Query: 324 VRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAAS 383
           VRAGLT K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP+  S
Sbjct: 320 VRAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKS 379

Query: 384 VSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVLFVPAYMEVSITSGATELHMYRAG 443
             FP+VPGPS++LVI GKG + TG S +  +  G+VLFVPA +E+ +T  +  + +YRAG
Sbjct: 380 TVFPAVPGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAG 439

Query: 444 VNNRFFGDL 453
           V++RFF  L
Sbjct: 440 VSSRFFQTL 441

BLAST of Cp4.1LG13g06820 vs. TAIR 10
Match: AT3G02570.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 462.2 bits (1188), Expect = 4.7e-130
Identity = 238/429 (55.48%), Postives = 313/429 (72.96%), Query Frame = 0

Query: 20  ADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTPDPRECYAEFWIGTHKSGPS 79
           ADRR RL RL+C V++YDWG IG DS V R+++ NS     DP   YAE W+GTH+SGPS
Sbjct: 15  ADRR-RLRRLRCSVKDYDWGKIGSDSLVYRVYAANSDYEI-DPTRPYAELWMGTHESGPS 74

Query: 80  FVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSVEKALS 139
           ++           A GSN V+L+ WI+ +P + LG++V  KWG DLPFLFKVLSV + LS
Sbjct: 75  YL---------EDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLS 134

Query: 140 IQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKGVLSSVPEIV 199
           IQAHPDK+LA+ +H+A P++YKDDNHKPEMALA T FEALCGFI  +ELK V+ ++PEI 
Sbjct: 135 IQAHPDKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIE 194

Query: 200 ELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSFEKKKR 259
           ELV   +A +    +  D +EKVK +  +IF+ +MS++    ++ + KLKRRL  E ++R
Sbjct: 195 ELVGSEEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQER 254

Query: 260 QLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYVGPNEPHAYISGECIECMAT 319
           QL+ KE+L+L+LE QYP D+G+++ FF NYVKL PGEALY+G NEPHAY+ GEC+E MAT
Sbjct: 255 QLTDKERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMAT 314

Query: 320 SDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILP 379
           SDNVVRAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP
Sbjct: 315 SDNVVRAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLP 374

Query: 380 QAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVLFVPAYMEVSITSGATELHM 439
             AS  FPSVPGPSL LV+ G+G + T  S +  +  G+VLFVPA  E+ + S +++L +
Sbjct: 375 SGASTVFPSVPGPSLLLVLQGEGRMSTEASADG-ISMGDVLFVPADTEIHLRS-SSDLKL 428

Query: 440 YRAGVNNRF 449
           YRAG+N+RF
Sbjct: 435 YRAGINSRF 428

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FZH53.9e-12953.61Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=... [more]
Q9M8846.6e-12955.48Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=... [more]
Q54PA05.5e-7538.53Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=m... [more]
Q9HFU41.5e-7240.14Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotyp... [more]
Q9GP384.4e-7239.39Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN... [more]
Match NameE-valueIdentityDescription
XP_023549674.10.0100.00mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6579210.10.098.45Mannose-6-phosphate isomerase 2, partial [Cucurbita argyrosperma subsp. sororia]... [more]
XP_022938390.10.098.23mannose-6-phosphate isomerase 1-like [Cucurbita moschata][more]
XP_022994025.10.098.23mannose-6-phosphate isomerase 1-like [Cucurbita maxima][more]
XP_038875726.11.90e-29891.59mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875727.1 mannose... [more]
Match NameE-valueIdentityDescription
A0A6J1FJN30.098.23Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3... [more]
A0A6J1K0470.098.23Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 S... [more]
A0A6J1E4R04.01e-29590.27Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3... [more]
A0A6J1I4438.09e-29590.04Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 S... [more]
A0A0A0KL161.55e-29089.60Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G67070.12.8e-13053.61Mannose-6-phosphate isomerase, type I [more]
AT3G02570.14.7e-13055.48Mannose-6-phosphate isomerase, type I [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 254..274
NoneNo IPR availableGENE3D1.10.441.10Phosphomannose Isomerase, domain 2coord: 184..350
e-value: 1.9E-131
score: 441.1
NoneNo IPR availablePIRSRPIRSR001480-2PIRSR001480-2coord: 34..443
e-value: 1.5E-123
score: 410.8
NoneNo IPR availablePIRSRPIRSR001480-1PIRSR001480-1coord: 34..443
e-value: 1.5E-123
score: 410.8
NoneNo IPR availablePANTHERPTHR10309:SF10MANNOSE-6-PHOSPHATE ISOMERASEcoord: 16..450
NoneNo IPR availableCDDcd02208cupin_RmlC-likecoord: 394..440
e-value: 4.98784E-5
score: 39.3882
NoneNo IPR availableCDDcd07011cupin_PMI_type_I_Ncoord: 29..344
e-value: 2.12042E-115
score: 336.829
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 325..344
score: 52.0
coord: 29..47
score: 45.79
coord: 306..325
score: 70.0
coord: 65..80
score: 53.12
coord: 160..183
score: 68.33
coord: 287..306
score: 49.5
coord: 271..286
score: 38.12
IPR016305Mannose-6-phosphate isomerasePIRSFPIRSF001480PMIcoord: 23..449
e-value: 4.1E-127
score: 422.8
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 16..450
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 30..326
e-value: 1.9E-131
score: 441.1
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 26..442
e-value: 1.9E-131
score: 441.1
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 26..407
e-value: 1.8E-100
score: 336.7
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMTIGR00218TIGR00218coord: 26..434
e-value: 1.0E-76
score: 256.4
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 160..168
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 25..445

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g06820.1Cp4.1LG13g06820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000032 cell wall mannoprotein biosynthetic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006486 protein glycosylation
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0008270 zinc ion binding