Cp4.1LG13g04620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG13g04620
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	E3 ubiquitin-protein ligase RING1-like
Location	Cp4.1LG13: 5974034 .. 5974714 (-)
RNA-Seq Expression	Cp4.1LG13g04620
Synteny	Cp4.1LG13g04620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGGATACTCTCTACGAATACCAGATATGGCAACCTGAAGAAGGTGAAGATGATATTAATTTTGCCATTTCCAATTCTCACTTGCTTCCCTGTGACTGTTTCCTTTTTCACCTTCGCTTTCGCCATGTTCTTCAGTCAAATCACTCTCAAGAATTTCACACTCTCCTAAATTCCTCCCTATCTTCGTCCTTGATACCTTGCGATGTCTTTCTCGCTAGTGGCTCCAGTATCTTGATTGCTGTTTTCTCCGACACTGGCGTCGCGCAAGACTTCTTGGATGCGGCAGTACCGGAGGTTCTGTCCGTTGCGGCTGATATCGTTTGCAATCCTCTTAATGATGGCCGTCGCATATTTCCGTTGGCGGTGTCTGTTTTAGTCGTTTCGCTGTACAATGAACGCGAGGAAATTGGGCGAGTTTTGCGGGAATCATCAGAGCAAGCGTTTAAAGCGTCGCCGGCGTGTGCGTCGGCTATCGAAGGTTTGAAGAACGTGGAGATTGATGTCCGTGGAGAGCTGTTGATCGGAGAATGCAGAATATGTTTGGATGAATTGATGAACGGAATGGAAGTTACGCAATTGCCGTGCGATCATTTTTACCACAGAGAGTGTATCGTCAAATGGCTAGAGACGAGTCATTTGTGCCCGTTGTGCCGCTACGCAATGCCATTGAGCTGA

mRNA sequence

ATGGCCATGGATACTCTCTACGAATACCAGATATGGCAACCTGAAGAAGGTGAAGATGATATTAATTTTGCCATTTCCAATTCTCACTTGCTTCCCTGTGACTGTTTCCTTTTTCACCTTCGCTTTCGCCATGTTCTTCAGTCAAATCACTCTCAAGAATTTCACACTCTCCTAAATTCCTCCCTATCTTCGTCCTTGATACCTTGCGATGTCTTTCTCGCTAGTGGCTCCAGTATCTTGATTGCTGTTTTCTCCGACACTGGCGTCGCGCAAGACTTCTTGGATGCGGCAGTACCGGAGGTTCTGTCCGTTGCGGCTGATATCGTTTGCAATCCTCTTAATGATGGCCGTCGCATATTTCCGTTGGCGGTGTCTGTTTTAGTCGTTTCGCTGTACAATGAACGCGAGGAAATTGGGCGAGTTTTGCGGGAATCATCAGAGCAAGCGTTTAAAGCGTCGCCGGCGTGTGCGTCGGCTATCGAAGGTTTGAAGAACGTGGAGATTGATGTCCGTGGAGAGCTGTTGATCGGAGAATGCAGAATATGTTTGGATGAATTGATGAACGGAATGGAAGTTACGCAATTGCCGTGCGATCATTTTTACCACAGAGAGTGTATCGTCAAATGGCTAGAGACGAGTCATTTGTGCCCGTTGTGCCGCTACGCAATGCCATTGAGCTGA

Coding sequence (CDS)

ATGGCCATGGATACTCTCTACGAATACCAGATATGGCAACCTGAAGAAGGTGAAGATGATATTAATTTTGCCATTTCCAATTCTCACTTGCTTCCCTGTGACTGTTTCCTTTTTCACCTTCGCTTTCGCCATGTTCTTCAGTCAAATCACTCTCAAGAATTTCACACTCTCCTAAATTCCTCCCTATCTTCGTCCTTGATACCTTGCGATGTCTTTCTCGCTAGTGGCTCCAGTATCTTGATTGCTGTTTTCTCCGACACTGGCGTCGCGCAAGACTTCTTGGATGCGGCAGTACCGGAGGTTCTGTCCGTTGCGGCTGATATCGTTTGCAATCCTCTTAATGATGGCCGTCGCATATTTCCGTTGGCGGTGTCTGTTTTAGTCGTTTCGCTGTACAATGAACGCGAGGAAATTGGGCGAGTTTTGCGGGAATCATCAGAGCAAGCGTTTAAAGCGTCGCCGGCGTGTGCGTCGGCTATCGAAGGTTTGAAGAACGTGGAGATTGATGTCCGTGGAGAGCTGTTGATCGGAGAATGCAGAATATGTTTGGATGAATTGATGAACGGAATGGAAGTTACGCAATTGCCGTGCGATCATTTTTACCACAGAGAGTGTATCGTCAAATGGCTAGAGACGAGTCATTTGTGCCCGTTGTGCCGCTACGCAATGCCATTGAGCTGA

Protein sequence

MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNSSLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIFPLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECRICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS

Homology

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.1e-13
Identity = 31/92 (33.70%), Postives = 57/92 (61.96%), Query Frame = 0


Query: 136 EEIGRVLRESSEQAFKASPACASAIEGLKNVEID---VRGELLIGECRICLDELMNGMEV 195
           E++ + L E+    +   PA  SAI+ L  V++    ++ E+   +C +C+DE  +G +V
Sbjct: 172 EQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEM--NQCAVCMDEFEDGSDV 231

Query: 196 TQLPCDHFYHRECIVKWLETSHLCPLCRYAMP 225
            Q+PC H +H++C++ WLE  + CP+CR+ +P
Sbjct: 232 KQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 8.7e-13
Identity = 33/92 (35.87%), Postives = 53/92 (57.61%), Query Frame = 0


Query: 136 EEIGRVLRESSEQAFKASPACASAIEGLKNVEI---DVRGELLIGECRICLDELMNGMEV 195
           E++ + L E+    +   PA  SAIE L  V I   ++  E    +C +C+D+   G E 
Sbjct: 180 EQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSE--FNQCAVCMDDFEEGTEA 239

Query: 196 TQLPCDHFYHRECIVKWLETSHLCPLCRYAMP 225
            Q+PC H YH++C++ WLE  + CP+CR+ +P
Sbjct: 240 KQMPCKHLYHKDCLLPWLELHNSCPVCRHELP 269

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.2e-11
Identity = 31/83 (37.35%), Postives = 45/83 (54.22%), Query Frame = 0


Query: 142 LRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECRICLDELMNGMEVTQLPCDHFY 201
           L ES        PA   A+E L  V+I+      +  C +CLD+L  G +  Q+PC+H +
Sbjct: 167 LAESDPSRNGTPPAKKEAVEALPTVKIEE-----VVSCSVCLDDLEVGSQAKQMPCEHKF 226

Query: 202 HRECIVKWLETSHLCPLCRYAMP 225
           H  CI+ WLE    CP+CR+ +P
Sbjct: 227 HSSCILPWLELHSSCPVCRFELP 244

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-10
Identity = 31/88 (35.23%), Postives = 49/88 (55.68%), Query Frame = 0


Query: 142 LRESSEQAFKASPACASAIEGLKNVEI-----DVRGELLIGECRICLDELMNGMEVTQLP 201
           LR   ++     PA  +A+  L+ VE+        GE+   EC IC +E+  G +V ++P
Sbjct: 175 LRRRDQKEGYQVPAARAAVVALRAVEVFNVAASNAGEV---ECVICKEEMSEGRDVCEMP 234

Query: 202 CDHFYHRECIVKWLETSHLCPLCRYAMP 225
           C HF+H +CI+ WL   + CP CR+ +P
Sbjct: 235 CQHFFHWKCILPWLSKKNTCPFCRFQLP 259

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 29/72 (40.28%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 152 ASPACASAIEGLKNVEIDVRGELLIGECRICLDELMNGMEVTQLPCDHFYHRECIVKWLE 211
           A PA  SAIE L+  E+      ++  C +C D ++ G    +LPC H YH +CIV WL 
Sbjct: 230 APPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLG 289

Query: 212 TSHLCPLCRYAM 224
           T + CP+CR+ +
Sbjct: 290 TRNSCPVCRFQL 301

[Close]

BLAST of Cp4.1LG13g04620 vs. NCBI nr
Match: XP_023550658.1 (uncharacterized protein LOC111808739 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461 bits (1186), Expect = 7.70e-164
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. NCBI nr
Match: KAG7016485.1 (E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 455 bits (1170), Expect = 2.12e-161
Identity = 223/226 (98.67%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFS TGVAQDFLDAAVPEVLSVAAD+VCNPLN GRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. NCBI nr
Match: XP_022939153.1 (uncharacterized protein LOC111445148 [Cucurbita moschata])

HSP 1 Score: 453 bits (1165), Expect = 1.23e-160
Identity = 222/226 (98.23%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFS TGVAQDFLDAAVPEVLSVAAD+VCNPLN GRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELM+GMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMSGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. NCBI nr
Match: KAG6578963.1 (E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 452 bits (1162), Expect = 3.52e-160
Identity = 222/226 (98.23%), Postives = 223/226 (98.67%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDI FAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDIYFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFS TGVAQDFLDAAVPEVLSVAAD+VCNPLN GRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. NCBI nr
Match: XP_022992996.1 (uncharacterized protein LOC111489156 [Cucurbita maxima])

HSP 1 Score: 448 bits (1152), Expect = 1.18e-158
Identity = 220/226 (97.35%), Postives = 222/226 (98.23%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFS TGVAQDFLDAAVP+VLSVAADIVCNPLN GRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKN+EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVTQLPC HFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy TrEMBL
Match: A0A6J1FKV9 (uncharacterized protein LOC111445148 OS=Cucurbita moschata OX=3662 GN=LOC111445148 PE=4 SV=1)

HSP 1 Score: 453 bits (1165), Expect = 5.94e-161
Identity = 222/226 (98.23%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFS TGVAQDFLDAAVPEVLSVAAD+VCNPLN GRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELM+GMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMSGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy TrEMBL
Match: A0A6J1JV38 (uncharacterized protein LOC111489156 OS=Cucurbita maxima OX=3661 GN=LOC111489156 PE=4 SV=1)

HSP 1 Score: 448 bits (1152), Expect = 5.70e-159
Identity = 220/226 (97.35%), Postives = 222/226 (98.23%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFS TGVAQDFLDAAVP+VLSVAADIVCNPLN GRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKN+EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVTQLPC HFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy TrEMBL
Match: A0A6J1HJY0 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC111463602 PE=4 SV=1)

HSP 1 Score: 398 bits (1022), Expect = 3.74e-139
Identity = 195/226 (86.28%), Postives = 201/226 (88.94%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLY+YQIWQPEE EDD+N  ISNSHLLPCDCFLFHL FRHVLQSNH QE   LLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSS LIPCD F ASG SILIAVFSDTGVAQDFLDAAVP+VLSVAADI CNPLN GRR+ 
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVL VS YNEREEIGRVLRESS Q FK SPA  SAIEGLK VEIDV GELLIGECR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVT+LPC HFYHR+CIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy TrEMBL
Match: A0A6J1JSE1 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111487409 PE=4 SV=1)

HSP 1 Score: 397 bits (1021), Expect = 5.31e-139
Identity = 195/226 (86.28%), Postives = 201/226 (88.94%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLY+YQIWQPEE EDD+N  ISNSHLLPCDCFLFHL FRHVLQSNH QE   LLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSS LIPCD F ASG SILIAVFSDTGVAQDFLDAAVP+VLSVAADI CNPLN GRR+ 
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVL VS YNEREEIGRVLRESS QAFK SPA  SAIEGLK VEIDV GELLIGECR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVT+LPC H YHR+CIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. ExPASy TrEMBL
Match: A0A0A0KDU3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 PE=4 SV=1)

HSP 1 Score: 392 bits (1007), Expect = 7.23e-137
Identity = 191/226 (84.51%), Postives = 201/226 (88.94%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLY+Y IWQPEEGEDD+N  ISNSHLLPC+CFLFHL FRH+LQSNHSQ+FH LLNS
Sbjct: 1   MAMDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQFHYLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120
           SLSS LIPCDVF A+ SSILIAVFS TGVAQDFLD AVP+VLSVAADI CNPLN GR I 
Sbjct: 61  SLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTIL 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180
           PLAVSVLVVS YNER+EIGRVLRESS QAFK  PA   AIEGLK VEIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKKVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226
           ICLDELMNGMEVT+LPC H YHR+CIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of Cp4.1LG13g04620 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.6e-14
Identity = 31/92 (33.70%), Postives = 57/92 (61.96%), Query Frame = 0


Query: 136 EEIGRVLRESSEQAFKASPACASAIEGLKNVEID---VRGELLIGECRICLDELMNGMEV 195
           E++ + L E+    +   PA  SAI+ L  V++    ++ E+   +C +C+DE  +G +V
Sbjct: 172 EQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEM--NQCAVCMDEFEDGSDV 231

Query: 196 TQLPCDHFYHRECIVKWLETSHLCPLCRYAMP 225
            Q+PC H +H++C++ WLE  + CP+CR+ +P
Sbjct: 232 KQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of Cp4.1LG13g04620 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.2e-12
Identity = 34/73 (46.58%), Postives = 45/73 (61.64%), Query Frame = 0


Query: 154 PACASAIEGLKNVEIDVRGELLIGE--CRICLDELMNGMEVTQLPCDHFYHRECIVKWLE 213
           PA  S I+ L  VE+ V  EL  G   C +C DE++   +V +LPC HFYH ECI+ WL 
Sbjct: 309 PAAKSVIQDLPVVELAVE-ELDKGNNVCAVCKDEMLVEEKVRRLPCSHFYHGECIIPWLG 368

Query: 214 TSHLCPLCRYAMP 225
             + CP+CRY +P
Sbjct: 369 IRNTCPVCRYELP 380

[Close]

BLAST of Cp4.1LG13g04620 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-12
Identity = 30/73 (41.10%), Postives = 40/73 (54.79%), Query Frame = 0


Query: 153 SPACASAIEGLKNVEIDVRGELLIGECRICLDELMNGMEVTQLPCDHFYHRECIVKWLET 212
           SPA  S +E +  V I    E   G C ICLDE   G    ++PC H +H +C+ +WL  
Sbjct: 83  SPALKSEVENMPRVVIGEDKEKYGGSCAICLDEWSKGDVAAEMPCKHKFHSKCVEEWLGR 142

Query: 213 SHLCPLCRYAMPL 226
              CP+CRY MP+
Sbjct: 143 HATCPMCRYEMPV 155

[Close]

BLAST of Cp4.1LG13g04620 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-12
Identity = 31/85 (36.47%), Postives = 47/85 (55.29%), Query Frame = 0


Query: 140 RVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECRICLDELMNGMEVTQLPCDH 199
           R+        +   PA   A+E L  V+I+    LL  +C +CLD+   GME  ++PC H
Sbjct: 190 RLAENDLNNRYGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKH 249

Query: 200 FYHRECIVKWLETSHLCPLCRYAMP 225
            +H +C++ WLE    CP+CRY +P
Sbjct: 250 KFHSDCLLPWLELHSSCPVCRYLLP 270

[Close]

BLAST of Cp4.1LG13g04620 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.4e-12
Identity = 31/85 (36.47%), Postives = 47/85 (55.29%), Query Frame = 0


Query: 140 RVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECRICLDELMNGMEVTQLPCDH 199
           R+        +   PA   A+E L  V+I+    LL  +C +CLD+   GME  ++PC H
Sbjct: 190 RLAENDLNNRYGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKH 249

Query: 200 FYHRECIVKWLETSHLCPLCRYAMP 225
            +H +C++ WLE    CP+CRY +P
Sbjct: 250 KFHSDCLLPWLELHSSCPVCRYLLP 270

The following BLAST results are available for this feature:

BLAST of Cp4.1LG13g04620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	5.1e-13	33.70	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	8.7e-13	35.87	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q6AVN2	8.2e-11	37.35	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q8GXF8	1.4e-10	35.23	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9SPL2	1.8e-10	40.28	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV...	[more]

BLAST of Cp4.1LG13g04620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023550658.1	7.70e-164	100.00	uncharacterized protein LOC111808739 [Cucurbita pepo subsp. pepo]	[more]
KAG7016485.1	2.12e-161	98.67	E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma ...	[more]
XP_022939153.1	1.23e-160	98.23	uncharacterized protein LOC111445148 [Cucurbita moschata]	[more]
KAG6578963.1	3.52e-160	98.23	E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma ...	[more]
XP_022992996.1	1.18e-158	97.35	uncharacterized protein LOC111489156 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG13g04620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FKV9	5.94e-161	98.23	uncharacterized protein LOC111445148 OS=Cucurbita moschata OX=3662 GN=LOC1114451...	[more]
A0A6J1JV38	5.70e-159	97.35	uncharacterized protein LOC111489156 OS=Cucurbita maxima OX=3661 GN=LOC111489156...	[more]
A0A6J1HJY0	3.74e-139	86.28	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JSE1	5.31e-139	86.28	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0KDU3	7.23e-137	84.51	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 P...	[more]

BLAST of Cp4.1LG13g04620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	3.6e-14	33.70	RING/U-box superfamily protein	[more]
AT3G02340.1	1.2e-12	46.58	RING/U-box superfamily protein	[more]
AT1G14200.1	3.4e-12	41.10	RING/U-box superfamily protein	[more]
AT3G13430.1	3.4e-12	36.47	RING/U-box superfamily protein	[more]
AT3G13430.2	3.4e-12	36.47	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 179..219 e-value: 8.7E-7 score: 38.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 178..220 e-value: 7.6E-14 score: 51.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 179..220 score: 12.902362
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 178..220 e-value: 0.0011 score: 15.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 141..225 e-value: 9.5E-19 score: 68.9
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 93..224
None	No IPR available	PANTHER	PTHR15710:SF120	F6A14.12 PROTEIN-RELATED	coord: 93..224
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 167..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG13g04620.1	Cp4.1LG13g04620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding