Cp4.1LG13g02830 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG13g02830
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMembrane-associated kinase regulator, putative
LocationCp4.1LG13: 2220087 .. 2221236 (-)
RNA-Seq ExpressionCp4.1LG13g02830
SyntenyCp4.1LG13g02830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCATCTCACCATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTACGGAAACAAGAACAAGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTTTCTCTGTTTCCGCCGGCAAAATTCAAAGCCCAGAACAGAGGCCTCAAATTCCGACCAAATTAGGGGAACACATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTTCCAATCGAACACACTTTGAAGCCCCAATCATCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGTCCAAGAACAGAGCACCCGCTGGCCCAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTCACCAGAGACAACAGCATAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATGTGGGGTTCCGGCTCCATCTCCGGCGTGGCGGTTCTCAAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCTGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTCGGTTGGTCTGTAAGCATCTGGGTAGGAGCAAATCGGCCTCAGCAGCTAGCGGATTGGCGGCGGTGGCTCCTCCGCCGAACCGGAGGGATGATTCGTTATTACAGCAACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGGATCCACAGGTAAAAACAAAGAAAAGAAACCTCTTTTAAACTTATAATTAGGTCATTGATTTTTGAATTATGTTTTTGTGGTCAGAGAAACCTGTGTTGGGTGTGGACGTCTGAGAGAGGTGTTTCAGAGTATTATGTTCTTGTTCGATTCCATTTTCCGCCATTGATTTGGAAGCAAAGAGCTTCAAAATAATAAAATCTGAGAGAAACCCAGAAGAGGAAGAACATTGAAAAGAAGAGAAATTGTTGGGTGATGAAAGTGATGATCAACAATACAAATGGTGTTTGGGAGTTGGGAATATGTAAAAGGCTCATAATTATTTATCAAAGGAATTTAAAAAGATATCTGATTC

mRNA sequence

CTCTCATCTCACCATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTACGGAAACAAGAACAAGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTTTCTCTGTTTCCGCCGGCAAAATTCAAAGCCCAGAACAGAGGCCTCAAATTCCGACCAAATTAGGGGAACACATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTTCCAATCGAACACACTTTGAAGCCCCAATCATCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGTCCAAGAACAGAGCACCCGCTGGCCCAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTCACCAGAGACAACAGCATAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATGTGGGGTTCCGGCTCCATCTCCGGCGTGGCGGTTCTCAAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCTGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTCGGTTGGTCTGTAAGCATCTGGGTAGGAGCAAATCGGCCTCAGCAGCTAGCGGATTGGCGGCGGTGGCTCCTCCGCCGAACCGGAGGGATGATTCGTTATTACAGCAACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGGATCCACAGAGAAACCTGTGTTGGGTGTGGACGTCTGAGAGAGGTGTTTCAGAGTATTATGTTCTTGTTCGATTCCATTTTCCGCCATTGATTTGGAAGCAAAGAGCTTCAAAATAATAAAATCTGAGAGAAACCCAGAAGAGGAAGAACATTGAAAAGAAGAGAAATTGTTGGGTGATGAAAGTGATGATCAACAATACAAATGGTGTTTGGGAGTTGGGAATATGTAAAAGGCTCATAATTATTTATCAAAGGAATTTAAAAAGATATCTGATTC

Coding sequence (CDS)

ATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTACGGAAACAAGAACAAGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTTTCTCTGTTTCCGCCGGCAAAATTCAAAGCCCAGAACAGAGGCCTCAAATTCCGACCAAATTAGGGGAACACATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTTCCAATCGAACACACTTTGAAGCCCCAATCATCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGTCCAAGAACAGAGCACCCGCTGGCCCAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTCACCAGAGACAACAGCATAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATGTGGGGTTCCGGCTCCATCTCCGGCGTGGCGGTTCTCAAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCTGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTCGGTTGGTCTGTAAGCATCTGGGTAGGAGCAAATCGGCCTCAGCAGCTAGCGGATTGGCGGCGGTGGCTCCTCCGCCGAACCGGAGGGATGATTCGTTATTACAGCAACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGGATCCACAGAGAAACCTGTGTTGGGTGTGGACGTCTGA

Protein sequence

MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPTKLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGPEQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAAVAPPPNRRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV
Homology
BLAST of Cp4.1LG13g02830 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.8e-20
Identity = 82/247 (33.20%), Postives = 120/247 (48.58%), Query Frame = 0

Query: 31  EEEDSFFDLELFSVSAGKIQSPEQRPQIPTKLGEHMNLCSDSISKRKILP-IEHTLKPQS 90
           E EDSFF+L+   +S    ++P+++ ++ T         S   +K K+LP ++ + KPQ 
Sbjct: 45  EGEDSFFELK---ISLSDFKTPKEKQRLETTTTTTTYSVS---NKSKVLPFVDISSKPQQ 104

Query: 91  S-IQFLKSAPKFTDFIFKKQRSMSKNRAPAGPEQEETRILSSKF-----TRDNSIRRIRG 150
           S    LKS  KF  F FKK  S          E+     L+ +F     T   S R+   
Sbjct: 105 SPTTLLKSGQKFRAFSFKK--SEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTAS 164

Query: 151 IFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSP-VREKLE 210
           I           +    F     +++  LIKP   K          ++   SP  REK  
Sbjct: 165 I------ARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREKQR 224

Query: 211 NGLPTGIRLVCKHLGRSKSASAASGLAAVAPPPNRRDDSLLQQHDGIESAILHCKRSLNG 270
           + +P+G+R V + LG+S+SASAA G  +   P NR D+SL  Q DGI+SAILHCK+S +G
Sbjct: 225 SNIPSGMRSVRRQLGKSRSASAAIGGMS---PANRIDESLQVQQDGIQSAILHCKKSFHG 274

BLAST of Cp4.1LG13g02830 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-13
Identity = 48/128 (37.50%), Postives = 66/128 (51.56%), Query Frame = 0

Query: 166 VQKYLKLIKPKLLKKPTDSGGEL--------------SSAAVDSPVREKLEN------GL 225
           +QKYLK +KP  ++     G +L              ++   DSPV  K  N       +
Sbjct: 267 MQKYLKKVKPLYIRVSRRYGEKLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINI 326

Query: 226 PTGIRLVCKHLGRSKSA-------SAASGLAAVAPPPNRRDDSLLQQHDGIESAILHCKR 267
           P G+++V KHLG+S+S+       S+ +          RRDDSLLQQ D I+SAILHCKR
Sbjct: 327 PAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKR 386

BLAST of Cp4.1LG13g02830 vs. NCBI nr
Match: XP_023550940.1 (probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535 bits (1378), Expect = 1.55e-191
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60
           MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT
Sbjct: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120
           KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP
Sbjct: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120

Query: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK
Sbjct: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAAVAPPPNRRDD 240
           PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAAVAPPPNRRDD
Sbjct: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAAVAPPPNRRDD 240

Query: 241 SLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273
           SLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 SLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273

BLAST of Cp4.1LG13g02830 vs. NCBI nr
Match: KAG6579360.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 496 bits (1278), Expect = 4.52e-176
Identity = 262/287 (91.29%), Postives = 264/287 (91.99%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEE----DSFFDLELFSVSAGKIQSPEQRP 60
           MDSVAYLKFFKFSGADGL KQEQEEEEEEEEEE    DSFFDLELFSVSAG IQSPEQRP
Sbjct: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEEEEDSFFDLELFSVSAGGIQSPEQRP 60

Query: 61  QIPTKLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRA 120
           QIP KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRSM+KNRA
Sbjct: 61  QIPIKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA 120

Query: 121 PAGPEQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK 180
           P G EQEETRILSSKFTRDNS RRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK
Sbjct: 121 PTGTEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK 180

Query: 181 LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA------ 240
           LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA      
Sbjct: 181 LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAAASGLAA 240

Query: 241 ----VAPPPNRRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273
               VAPPPNRRDDSLL+QHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 AAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 287

BLAST of Cp4.1LG13g02830 vs. NCBI nr
Match: KAG7016845.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 493 bits (1269), Expect = 7.65e-175
Identity = 260/278 (93.53%), Postives = 262/278 (94.24%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEE-DSFFDLELFSVSAGKIQSPEQRPQIP 60
           MDSVAYLKFFKFSGADGL KQEQEEEEEEEEEE DSFFDLEL SVSAG IQSPEQRPQIP
Sbjct: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60

Query: 61  TKLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAG 120
            KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRSM+KNRAP G
Sbjct: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120

Query: 121 PEQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            EQEETRILSSKFTRDNS RRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA----VAPPP 240
           KPTDSGGELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSASAASGLAA    VAPPP
Sbjct: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240

Query: 241 NRRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273
           NRRDDSLL+QHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 278

BLAST of Cp4.1LG13g02830 vs. NCBI nr
Match: XP_022922154.1 (probable membrane-associated kinase regulator 5 [Cucurbita moschata])

HSP 1 Score: 491 bits (1265), Expect = 2.79e-174
Identity = 257/277 (92.78%), Postives = 260/277 (93.86%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60
           MDSVAYLKFFKF+GADGL KQEQEEEEEEEE  DS FDLELFSVSAG IQSPEQRPQIPT
Sbjct: 1   MDSVAYLKFFKFAGADGLLKQEQEEEEEEEE--DSLFDLELFSVSAGGIQSPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120
           KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRS +KNRAPAG 
Sbjct: 61  KLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSTAKNRAPAGT 120

Query: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           EQEETRILSSKFTRDNS RRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK
Sbjct: 121 EQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA----VAPPPN 240
           PTD+G ELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASA SGLAA    VAPPPN
Sbjct: 181 PTDTGSELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASATSGLAAAAVAVAPPPN 240

Query: 241 RRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273
           RRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 RRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 275

BLAST of Cp4.1LG13g02830 vs. NCBI nr
Match: XP_022973059.1 (probable membrane-associated kinase regulator 5 [Cucurbita maxima])

HSP 1 Score: 489 bits (1259), Expect = 2.29e-173
Identity = 257/276 (93.12%), Postives = 260/276 (94.20%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60
           MDSVAYLKFFKFSGADGL KQEQ+EEEEEEEE DSFFDLEL SVSAGKIQ PEQRPQIPT
Sbjct: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEE-DSFFDLELLSVSAGKIQGPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120
           KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRSM+KNRAPAGP
Sbjct: 61  KLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGP 120

Query: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           EQEETRILSSKFTRDNS RRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLKK
Sbjct: 121 EQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA---VAPPPNR 240
           PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAAS LAA   VAPPPNR
Sbjct: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNR 240

Query: 241 RDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273
           RDDSLLQ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 RDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 275

BLAST of Cp4.1LG13g02830 vs. ExPASy TrEMBL
Match: A0A6J1E2K7 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111430217 PE=4 SV=1)

HSP 1 Score: 491 bits (1265), Expect = 1.35e-174
Identity = 257/277 (92.78%), Postives = 260/277 (93.86%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60
           MDSVAYLKFFKF+GADGL KQEQEEEEEEEE  DS FDLELFSVSAG IQSPEQRPQIPT
Sbjct: 1   MDSVAYLKFFKFAGADGLLKQEQEEEEEEEE--DSLFDLELFSVSAGGIQSPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120
           KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRS +KNRAPAG 
Sbjct: 61  KLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSTAKNRAPAGT 120

Query: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           EQEETRILSSKFTRDNS RRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK
Sbjct: 121 EQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA----VAPPPN 240
           PTD+G ELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASA SGLAA    VAPPPN
Sbjct: 181 PTDTGSELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASATSGLAAAAVAVAPPPN 240

Query: 241 RRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273
           RRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 RRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 275

BLAST of Cp4.1LG13g02830 vs. ExPASy TrEMBL
Match: A0A6J1IDF3 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111471559 PE=4 SV=1)

HSP 1 Score: 489 bits (1259), Expect = 1.11e-173
Identity = 257/276 (93.12%), Postives = 260/276 (94.20%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60
           MDSVAYLKFFKFSGADGL KQEQ+EEEEEEEE DSFFDLEL SVSAGKIQ PEQRPQIPT
Sbjct: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEE-DSFFDLELLSVSAGKIQGPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120
           KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRSM+KNRAPAGP
Sbjct: 61  KLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGP 120

Query: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           EQEETRILSSKFTRDNS RRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLKK
Sbjct: 121 EQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA---VAPPPNR 240
           PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAAS LAA   VAPPPNR
Sbjct: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNR 240

Query: 241 RDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273
           RDDSLLQ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 RDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 275

BLAST of Cp4.1LG13g02830 vs. ExPASy TrEMBL
Match: A0A0A0KK03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1)

HSP 1 Score: 416 bits (1068), Expect = 4.94e-144
Identity = 215/269 (79.93%), Postives = 234/269 (86.99%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60
           MDSVA+LKFFK SGAD L+KQE+EEEEEEEE EDSFFD+E FSVSAGKIQSPEQRPQIPT
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEGEDSFFDIEFFSVSAGKIQSPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120
           KLG+H+NLCSDSISKRKILPIE T KPQS IQ LKSAPKF  FIFKKQRSM+KNR P G 
Sbjct: 61  KLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRPPTGQ 120

Query: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           E  E+R+L+SK TRDNS RR  GIFPPDPC  P P+P  RFSKE VQKYLKLIKPK+L+K
Sbjct: 121 EGGESRVLNSKLTRDNSTRRFGGIFPPDPCEPPVPAPTRRFSKEVVQKYLKLIKPKVLRK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA-VAPPPNRRD 240
           PTDSG +LSSAAVDSPVREK ENGLP GIRLVC+HLG+SKSASAA GLAA V P  NRRD
Sbjct: 181 PTDSGSDLSSAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAAAVIPQTNRRD 240

Query: 241 DSLLQQHDGIESAILHCKRSLNGSTEKPV 268
           DSLLQQHDGI+SAILHCKRS N S+EKP+
Sbjct: 241 DSLLQQHDGIQSAILHCKRSFNASSEKPL 269

BLAST of Cp4.1LG13g02830 vs. ExPASy TrEMBL
Match: A0A1S3AUW4 (probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC103482925 PE=4 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 9.21e-141
Identity = 214/273 (78.39%), Postives = 236/273 (86.45%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEE-DSFFDLELFSVSAGKIQSPEQRPQIP 60
           MDSVA+LKFFK SGAD L+KQE+EEEEEEEEEE DSFFD+E FSVSAGKIQSPEQRPQIP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRPQIP 60

Query: 61  TKLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAG 120
           TKLG+H+NLCSDSISKRKILPIE T KPQS IQ LKSAPKF  FIFKKQRSM+KN+AP G
Sbjct: 61  TKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKAPTG 120

Query: 121 PEQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            E +++ + SSK TRDNS RR  GIFP DPC  P P+P  RFSKEAV+KY KLIKPK+L+
Sbjct: 121 QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRRFSKEAVRKYFKLIKPKVLR 180

Query: 181 KPTDSGGELSS---AAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA-VAPPP 240
           KPTDSG +LSS   AAVDSPVREK ENGLP GIRLVC+HLG+SKSASAA GLAA V PPP
Sbjct: 181 KPTDSGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAAAVVPPP 240

Query: 241 NRRDDSLLQQHDGIESAILHCKRSLNGSTEKPV 268
           NRRDDSLLQQHDGI+SAILHCKRS N S+EKP+
Sbjct: 241 NRRDDSLLQQHDGIQSAILHCKRSFNASSEKPL 273

BLAST of Cp4.1LG13g02830 vs. ExPASy TrEMBL
Match: A0A5D3C3T3 (Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00860 PE=4 SV=1)

HSP 1 Score: 402 bits (1034), Expect = 6.85e-139
Identity = 212/276 (76.81%), Postives = 235/276 (85.14%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEE----DSFFDLELFSVSAGKIQSPEQRP 60
           MDSVA+LKFFK SGAD L+KQE+EEEEEEEEEE    DSFFD+E FSVSAGKIQSPEQRP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRP 60

Query: 61  QIPTKLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRA 120
           QIPTKLG+H+NLCSDSISKRKILPIE T KPQS IQ LKSAPKF  FIFKKQRSM+KN+A
Sbjct: 61  QIPTKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKA 120

Query: 121 PAGPEQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK 180
           P   E +++ + SSK TRDNS RR  GIFP DPC  P P+P  RFSKEAV+KY KLIKPK
Sbjct: 121 PTAQEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRRFSKEAVRKYFKLIKPK 180

Query: 181 LLKKPTDSGGELSS---AAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAA-VA 240
           +L+KPTD+G +LSS   AAVDSPVREK ENGLP GIRLVC+HLG+SKSASAA GLAA V 
Sbjct: 181 VLRKPTDTGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAAAVV 240

Query: 241 PPPNRRDDSLLQQHDGIESAILHCKRSLNGSTEKPV 268
           PPPNRRDDSLLQQHDGI+SAILHCKRS N S+EKP+
Sbjct: 241 PPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPL 276

BLAST of Cp4.1LG13g02830 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 99.4 bits (246), Expect = 4.8e-21
Identity = 82/247 (33.20%), Postives = 120/247 (48.58%), Query Frame = 0

Query: 31  EEEDSFFDLELFSVSAGKIQSPEQRPQIPTKLGEHMNLCSDSISKRKILP-IEHTLKPQS 90
           E EDSFF+L+   +S    ++P+++ ++ T         S   +K K+LP ++ + KPQ 
Sbjct: 45  EGEDSFFELK---ISLSDFKTPKEKQRLETTTTTTTYSVS---NKSKVLPFVDISSKPQQ 104

Query: 91  S-IQFLKSAPKFTDFIFKKQRSMSKNRAPAGPEQEETRILSSKF-----TRDNSIRRIRG 150
           S    LKS  KF  F FKK  S          E+     L+ +F     T   S R+   
Sbjct: 105 SPTTLLKSGQKFRAFSFKK--SEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTAS 164

Query: 151 IFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSP-VREKLE 210
           I           +    F     +++  LIKP   K          ++   SP  REK  
Sbjct: 165 I------ARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREKQR 224

Query: 211 NGLPTGIRLVCKHLGRSKSASAASGLAAVAPPPNRRDDSLLQQHDGIESAILHCKRSLNG 270
           + +P+G+R V + LG+S+SASAA G  +   P NR D+SL  Q DGI+SAILHCK+S +G
Sbjct: 225 SNIPSGMRSVRRQLGKSRSASAAIGGMS---PANRIDESLQVQQDGIQSAILHCKKSFHG 274

BLAST of Cp4.1LG13g02830 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 77.0 bits (188), Expect = 2.6e-14
Identity = 48/128 (37.50%), Postives = 66/128 (51.56%), Query Frame = 0

Query: 166 VQKYLKLIKPKLLKKPTDSGGEL--------------SSAAVDSPVREKLEN------GL 225
           +QKYLK +KP  ++     G +L              ++   DSPV  K  N       +
Sbjct: 267 MQKYLKKVKPLYIRVSRRYGEKLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINI 326

Query: 226 PTGIRLVCKHLGRSKSA-------SAASGLAAVAPPPNRRDDSLLQQHDGIESAILHCKR 267
           P G+++V KHLG+S+S+       S+ +          RRDDSLLQQ D I+SAILHCKR
Sbjct: 327 PAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKR 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLX46.8e-2033.20Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ... [more]
Q9SH583.6e-1337.50Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_023550940.11.55e-191100.00probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo][more]
KAG6579360.14.52e-17691.29putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma... [more]
KAG7016845.17.65e-17593.53putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma... [more]
XP_022922154.12.79e-17492.78probable membrane-associated kinase regulator 5 [Cucurbita moschata][more]
XP_022973059.12.29e-17393.12probable membrane-associated kinase regulator 5 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E2K71.35e-17492.78probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1IDF31.11e-17393.12probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0KK034.94e-14479.93Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1[more]
A0A1S3AUW49.21e-14178.39probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3C3T36.85e-13976.81Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
AT5G52870.14.8e-2133.20unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G64080.12.6e-1437.50unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 15..35
NoneNo IPR availablePANTHERPTHR33929:SF4MEMBRANE-ASSOCIATED KINASE REGULATOR 5-RELATEDcoord: 1..266
IPR039619Membrane-associated kinase regulator 2/5PANTHERPTHR33929MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..266

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG13g02830.1Cp4.1LG13g02830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity