Cp4.1LG13g02440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG13g02440 vs. ExPASy Swiss-Prot
Match: Q9ZW85 (3-isopropylmalate dehydratase small subunit 1 OS=Arabidopsis thaliana OX=3702 GN=SSU1 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 6.3e-58
Identity = 123/195 (63.08%), Postives = 146/195 (74.87%), Query Frame = 0

Query: 13  ATSTPRSSPSISH--LSPPRTIKFPTFYSNFLNDLSTTVSH------APSRAASILPRAT 72
           A S   ++P++S    SP +   F TF S FL   ST+V+       +   ++S + R+ 
Sbjct: 2   AASLQSANPTLSRTLASPNKPSSFATFRSPFLRFNSTSVASNFKPLVSREASSSFVTRSA 61

Query: 73  ATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSY 132
           A P    +    FHG CYVVGDNIDTDQIIPAE+LTLVPSNP EYEKLGS+ALVGLP+SY
Sbjct: 62  AEPQERKT----FHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVGLPASY 121

Query: 133 STRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVAT 192
             RFV+  EMKTKYSIII G+NFGCGSSREHAPV LGA+GA AVVA+SYARIFFRNSVAT
Sbjct: 122 KERFVQPGEMKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFFRNSVAT 181

Query: 193 GEIYPLESEIRVCDE 200
           GE+YPL+SE+RVCDE
Sbjct: 182 GEVYPLDSEVRVCDE 192

BLAST of Cp4.1LG13g02440 vs. ExPASy Swiss-Prot
Match: Q9LYT7 (3-isopropylmalate dehydratase small subunit 3 OS=Arabidopsis thaliana OX=3702 GN=SSU3 PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.7e-50
Identity = 106/162 (65.43%), Postives = 124/162 (76.54%), Query Frame = 0

Query: 42  LNDLSTTVSHAP--SRAASILPRATATPSSAASSST--VFHGFCYVVGDNIDTDQIIPAE 101
           L   ST  ++ P  S +A+I+ R  A+ S +  S T   FHG C+V+ DNIDTDQIIPAE
Sbjct: 34  LKSASTIFNYKPLTSSSATIITRVAASSSDSGESITRETFHGLCFVLKDNIDTDQIIPAE 93

Query: 102 YLTLVPSNPSEYEKLGSHALVGLPSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAP 161
           Y TL+PS P + EKLGS AL GLP  Y+ RFV   EMK+KYS+II GDNFGCGSSREHAP
Sbjct: 94  YGTLIPSIPEDREKLGSFALNGLPKFYNERFVVPGEMKSKYSVIIGGDNFGCGSSREHAP 153

Query: 162 VALGASGAVAVVAESYARIFFRNSVATGEIYPLESEIRVCDE 200
           V LGA+GA AVVAESYARIFFRN VATGEI+PLESE+R+CDE
Sbjct: 154 VCLGAAGAKAVVAESYARIFFRNCVATGEIFPLESEVRICDE 195

BLAST of Cp4.1LG13g02440 vs. ExPASy Swiss-Prot
Match: Q9ZW84 (3-isopropylmalate dehydratase small subunit 2 OS=Arabidopsis thaliana OX=3702 GN=SSU2 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.5e-46
Identity = 106/197 (53.81%), Postives = 136/197 (69.04%), Query Frame = 0

Query: 8   SVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDL---STTVSHAPSRAASILPRAT 67
           S+ TF  + P SS   S  S   T + P    N    L   S +++   + + +I+PRA 
Sbjct: 4   SLPTFPQALPCSSTKTS--SSLATFRSPFLRFNGSTSLIPSSISITSRGTSSPTIIPRAA 63

Query: 68  ATPSSA--ASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPS 127
           A+ S +  A ++T FHG CYV+ DNIDTDQIIPA      PSN  E +++ +HAL GLP 
Sbjct: 64  ASESDSNEALANTTFHGLCYVLKDNIDTDQIIPAGAACTFPSNQQERDEIAAHALSGLPD 123

Query: 128 SYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSV 187
            + TRF+E  E ++KYSIII G+NFGCGSSREHAPV LGA+GA A+VAESYARIFFRNSV
Sbjct: 124 FHKTRFIEPGENRSKYSIIIGGENFGCGSSREHAPVCLGAAGAKAIVAESYARIFFRNSV 183

Query: 188 ATGEIYPLESEIRVCDE 200
           ATGE++PLESE+RVC+E
Sbjct: 184 ATGEVFPLESEVRVCEE 198

BLAST of Cp4.1LG13g02440 vs. ExPASy Swiss-Prot
Match: B5YHE5 (3-isopropylmalate dehydratase small subunit OS=Thermodesulfovibrio yellowstonii (strain ATCC 51303 / DSM 11347 / YP87) OX=289376 GN=leuD PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.3e-18
Identity = 59/126 (46.83%), Postives = 75/126 (59.52%), Query Frame = 0

Query: 79  GFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSYSTRFVESNEMKTKY 138
           G  +  GDNIDTD IIPA YL     N S+ ++L  H +    SS+ ++    +      
Sbjct: 5   GKVWKFGDNIDTDAIIPARYL-----NTSDPKELAKHVMEDADSSFPSKVKPGD------ 64

Query: 139 SIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATG----EIYPLESEI 198
            IIIAG NFGCGSSREHAP+A+ ASG  AV+A+SYARIFFRN+   G    E+  L  E 
Sbjct: 65  -IIIAGRNFGCGSSREHAPIAIKASGIRAVIAKSYARIFFRNAFNIGLPIFEVPELIDET 118

Query: 199 RVCDEL 201
              DE+
Sbjct: 125 NEGDEV 118

BLAST of Cp4.1LG13g02440 vs. ExPASy Swiss-Prot
Match: Q8TX94 (3-isopropylmalate dehydratase small subunit 1 OS=Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) OX=190192 GN=leuD1 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.9e-18
Identity = 50/110 (45.45%), Postives = 66/110 (60.00%), Query Frame = 0

Query: 76  VFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSYSTRFVESNEMK 135
           V  G  +V GD+IDTDQIIP  YLT       + E+L  H + G    +  +  E +   
Sbjct: 4   VIRGRAWVFGDDIDTDQIIPGRYLT-----TQDPEELAKHVMEGADPEFPEKVREGD--- 63

Query: 136 TKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATG 186
               +I+AG NFGCGSSREHAP+AL A+G   VV  S+ARIF+RN++  G
Sbjct: 64  ----VIVAGKNFGCGSSREHAPIALKAAGIACVVTRSFARIFYRNAINLG 101

BLAST of Cp4.1LG13g02440 vs. NCBI nr
Match: XP_023550722.1 (3-isopropylmalate dehydratase small subunit 3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375 bits (962), Expect = 1.28e-129
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60
           MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL
Sbjct: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60

Query: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120
           PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL
Sbjct: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120

Query: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180
           PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN
Sbjct: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180

Query: 181 SVATGEIYPLESEIRVCDE 199
           SVATGEIYPLESEIRVCDE
Sbjct: 181 SVATGEIYPLESEIRVCDE 199

BLAST of Cp4.1LG13g02440 vs. NCBI nr
Match: XP_023550721.1 (3-isopropylmalate dehydratase small subunit 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375 bits (962), Expect = 3.19e-129
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60
           MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL
Sbjct: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60

Query: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120
           PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL
Sbjct: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120

Query: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180
           PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN
Sbjct: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180

Query: 181 SVATGEIYPLESEIRVCDE 199
           SVATGEIYPLESEIRVCDE
Sbjct: 181 SVATGEIYPLESEIRVCDE 199

BLAST of Cp4.1LG13g02440 vs. NCBI nr
Match: KAG7016880.1 (3-isopropylmalate dehydratase small subunit 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 360 bits (924), Expect = 1.00e-123
Identity = 192/199 (96.48%), Postives = 193/199 (96.98%), Query Frame = 0

Query: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60
           M TAP VSVS FATSTPRSSPSIS LSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL
Sbjct: 1   MATAPFVSVSPFATSTPRSSPSISLLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60

Query: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120
           PRATA PSSA SSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL
Sbjct: 61  PRATAAPSSAVSSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120

Query: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180
           PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN
Sbjct: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180

Query: 181 SVATGEIYPLESEIRVCDE 199
           SVATGEIYPLESEIR+CDE
Sbjct: 181 SVATGEIYPLESEIRICDE 199

BLAST of Cp4.1LG13g02440 vs. NCBI nr
Match: XP_022972989.1 (3-isopropylmalate dehydratase small subunit 3-like [Cucurbita maxima])

HSP 1 Score: 358 bits (920), Expect = 4.07e-123
Identity = 191/199 (95.98%), Postives = 192/199 (96.48%), Query Frame = 0

Query: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60
           M TA  VSVS FATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAA IL
Sbjct: 1   MATAAFVSVSPFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAAIIL 60

Query: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120
           PRATATPS A SSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL
Sbjct: 61  PRATATPSYAVSSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120

Query: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180
           PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN
Sbjct: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180

Query: 181 SVATGEIYPLESEIRVCDE 199
           SVATGEIYPLESEIR+CDE
Sbjct: 181 SVATGEIYPLESEIRICDE 199

BLAST of Cp4.1LG13g02440 vs. NCBI nr
Match: KAG6579402.1 (3-isopropylmalate dehydratase small subunit 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 358 bits (920), Expect = 4.07e-123
Identity = 191/199 (95.98%), Postives = 194/199 (97.49%), Query Frame = 0

Query: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60
           M TAP VSVS FATSTPRSSPSIS LSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL
Sbjct: 1   MATAPFVSVSPFATSTPRSSPSISLLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60

Query: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120
           PRATA+PSSA SSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL
Sbjct: 61  PRATASPSSAVSSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120

Query: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180
           PSSYSTRFVES+EMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN
Sbjct: 121 PSSYSTRFVESDEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180

Query: 181 SVATGEIYPLESEIRVCDE 199
           SVATGEIYPLESEIR+CDE
Sbjct: 181 SVATGEIYPLESEIRICDE 199

BLAST of Cp4.1LG13g02440 vs. ExPASy TrEMBL
Match: A0A6J1ID74 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucurbita maxima OX=3661 GN=LOC111471515 PE=4 SV=1)

HSP 1 Score: 358 bits (920), Expect = 1.97e-123
Identity = 191/199 (95.98%), Postives = 192/199 (96.48%), Query Frame = 0

Query: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60
           M TA  VSVS FATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAA IL
Sbjct: 1   MATAAFVSVSPFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAAIIL 60

Query: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120
           PRATATPS A SSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL
Sbjct: 61  PRATATPSYAVSSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120

Query: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180
           PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN
Sbjct: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180

Query: 181 SVATGEIYPLESEIRVCDE 199
           SVATGEIYPLESEIR+CDE
Sbjct: 181 SVATGEIYPLESEIRICDE 199

BLAST of Cp4.1LG13g02440 vs. ExPASy TrEMBL
Match: A0A6J1E3Q1 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucurbita moschata OX=3662 GN=LOC111430309 PE=4 SV=1)

HSP 1 Score: 360 bits (924), Expect = 2.32e-123
Identity = 192/199 (96.48%), Postives = 193/199 (96.98%), Query Frame = 0

Query: 1   MTTAPSVSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60
           M TAP VSVS FATSTPRSSPSIS LSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL
Sbjct: 1   MATAPFVSVSPFATSTPRSSPSISLLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASIL 60

Query: 61  PRATATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120
           PRATA PSSA SSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL
Sbjct: 61  PRATAAPSSAVSSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGL 120

Query: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180
           PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN
Sbjct: 121 PSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRN 180

Query: 181 SVATGEIYPLESEIRVCDE 199
           SVATGEIYPLESEIR+CDE
Sbjct: 181 SVATGEIYPLESEIRICDE 199

BLAST of Cp4.1LG13g02440 vs. ExPASy TrEMBL
Match: A0A5A7T900 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00620 PE=4 SV=1)

HSP 1 Score: 302 bits (773), Expect = 4.18e-101
Identity = 161/193 (83.42%), Postives = 174/193 (90.16%), Query Frame = 0

Query: 7   VSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASILPRATAT 66
           +S S  A STPRSSPS+S LS P+T+KFP+ YSN L DLS  VSHA  R A+I+PRATA+
Sbjct: 7   LSRSPIAISTPRSSPSVSLLSSPQTLKFPSSYSNSLFDLSIAVSHATPRLATIIPRATAS 66

Query: 67  PSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSYST 126
           PSS A S+T FHG CYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGS+AL+GLPSSYST
Sbjct: 67  PSSTAPSATAFHGRCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIGLPSSYST 126

Query: 127 RFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATGE 186
           RFVES+EMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATGE
Sbjct: 127 RFVESDEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATGE 186

Query: 187 IYPLESEIRVCDE 199
           IYPLESE R+CDE
Sbjct: 187 IYPLESETRICDE 199

BLAST of Cp4.1LG13g02440 vs. ExPASy TrEMBL
Match: A0A1S3AT32 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucumis melo OX=3656 GN=LOC103482595 PE=4 SV=1)

HSP 1 Score: 302 bits (773), Expect = 4.18e-101
Identity = 161/193 (83.42%), Postives = 174/193 (90.16%), Query Frame = 0

Query: 7   VSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASILPRATAT 66
           +S S  A STPRSSPS+S LS P+T+KFP+ YSN L DLS  VSHA  R A+I+PRATA+
Sbjct: 7   LSRSPIAISTPRSSPSVSLLSSPQTLKFPSSYSNSLFDLSIAVSHATPRLATIIPRATAS 66

Query: 67  PSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSYST 126
           PSS A S+T FHG CYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGS+AL+GLPSSYST
Sbjct: 67  PSSTAPSATAFHGRCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIGLPSSYST 126

Query: 127 RFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATGE 186
           RFVES+EMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATGE
Sbjct: 127 RFVESDEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATGE 186

Query: 187 IYPLESEIRVCDE 199
           IYPLESE R+CDE
Sbjct: 187 IYPLESETRICDE 199

BLAST of Cp4.1LG13g02440 vs. ExPASy TrEMBL
Match: A0A6J1H3H6 (3-isopropylmalate dehydratase small subunit 3-like OS=Cucurbita moschata OX=3662 GN=LOC111459316 PE=4 SV=1)

HSP 1 Score: 301 bits (772), Expect = 7.27e-101
Identity = 162/193 (83.94%), Postives = 176/193 (91.19%), Query Frame = 0

Query: 7   VSVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDLSTTVSHAPSRAASILPRATAT 66
           +S S  AT+T RSSP IS L PP++IKFP+F+ + L DLS T SHA  RAA+I+PRA AT
Sbjct: 9   LSQSPIATATRRSSPFISLL-PPQSIKFPSFHCHTLKDLSITASHASQRAATIIPRAAAT 68

Query: 67  PSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSYST 126
           PSSAASS+TVFHG CYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGS+AL+GLPSSYST
Sbjct: 69  PSSAASSTTVFHGSCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSYALIGLPSSYST 128

Query: 127 RFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVATGE 186
           RFVE NEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSV+TGE
Sbjct: 129 RFVEPNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVSTGE 188

Query: 187 IYPLESEIRVCDE 199
           IYPLESEIR+CDE
Sbjct: 189 IYPLESEIRICDE 200

BLAST of Cp4.1LG13g02440 vs. TAIR 10
Match: AT2G43090.1 (Aconitase/3-isopropylmalate dehydratase protein )

HSP 1 Score: 225.3 bits (573), Expect = 4.5e-59
Identity = 123/195 (63.08%), Postives = 146/195 (74.87%), Query Frame = 0

Query: 13  ATSTPRSSPSISH--LSPPRTIKFPTFYSNFLNDLSTTVSH------APSRAASILPRAT 72
           A S   ++P++S    SP +   F TF S FL   ST+V+       +   ++S + R+ 
Sbjct: 2   AASLQSANPTLSRTLASPNKPSSFATFRSPFLRFNSTSVASNFKPLVSREASSSFVTRSA 61

Query: 73  ATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSY 132
           A P    +    FHG CYVVGDNIDTDQIIPAE+LTLVPSNP EYEKLGS+ALVGLP+SY
Sbjct: 62  AEPQERKT----FHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVGLPASY 121

Query: 133 STRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVAT 192
             RFV+  EMKTKYSIII G+NFGCGSSREHAPV LGA+GA AVVA+SYARIFFRNSVAT
Sbjct: 122 KERFVQPGEMKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFFRNSVAT 181

Query: 193 GEIYPLESEIRVCDE 200
           GE+YPL+SE+RVCDE
Sbjct: 182 GEVYPLDSEVRVCDE 192

BLAST of Cp4.1LG13g02440 vs. TAIR 10
Match: AT3G58990.1 (isopropylmalate isomerase 1 )

HSP 1 Score: 200.7 bits (509), Expect = 1.2e-51
Identity = 106/162 (65.43%), Postives = 124/162 (76.54%), Query Frame = 0

Query: 42  LNDLSTTVSHAP--SRAASILPRATATPSSAASSST--VFHGFCYVVGDNIDTDQIIPAE 101
           L   ST  ++ P  S +A+I+ R  A+ S +  S T   FHG C+V+ DNIDTDQIIPAE
Sbjct: 34  LKSASTIFNYKPLTSSSATIITRVAASSSDSGESITRETFHGLCFVLKDNIDTDQIIPAE 93

Query: 102 YLTLVPSNPSEYEKLGSHALVGLPSSYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAP 161
           Y TL+PS P + EKLGS AL GLP  Y+ RFV   EMK+KYS+II GDNFGCGSSREHAP
Sbjct: 94  YGTLIPSIPEDREKLGSFALNGLPKFYNERFVVPGEMKSKYSVIIGGDNFGCGSSREHAP 153

Query: 162 VALGASGAVAVVAESYARIFFRNSVATGEIYPLESEIRVCDE 200
           V LGA+GA AVVAESYARIFFRN VATGEI+PLESE+R+CDE
Sbjct: 154 VCLGAAGAKAVVAESYARIFFRNCVATGEIFPLESEVRICDE 195

BLAST of Cp4.1LG13g02440 vs. TAIR 10
Match: AT2G43100.1 (isopropylmalate isomerase 2 )

HSP 1 Score: 187.6 bits (475), Expect = 1.0e-47
Identity = 106/197 (53.81%), Postives = 136/197 (69.04%), Query Frame = 0

Query: 8   SVSTFATSTPRSSPSISHLSPPRTIKFPTFYSNFLNDL---STTVSHAPSRAASILPRAT 67
           S+ TF  + P SS   S  S   T + P    N    L   S +++   + + +I+PRA 
Sbjct: 4   SLPTFPQALPCSSTKTS--SSLATFRSPFLRFNGSTSLIPSSISITSRGTSSPTIIPRAA 63

Query: 68  ATPSSA--ASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPS 127
           A+ S +  A ++T FHG CYV+ DNIDTDQIIPA      PSN  E +++ +HAL GLP 
Sbjct: 64  ASESDSNEALANTTFHGLCYVLKDNIDTDQIIPAGAACTFPSNQQERDEIAAHALSGLPD 123

Query: 128 SYSTRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSV 187
            + TRF+E  E ++KYSIII G+NFGCGSSREHAPV LGA+GA A+VAESYARIFFRNSV
Sbjct: 124 FHKTRFIEPGENRSKYSIIIGGENFGCGSSREHAPVCLGAAGAKAIVAESYARIFFRNSV 183

Query: 188 ATGEIYPLESEIRVCDE 200
           ATGE++PLESE+RVC+E
Sbjct: 184 ATGEVFPLESEVRVCEE 198

BLAST of Cp4.1LG13g02440 vs. TAIR 10
Match: AT2G43090.2 (Aconitase/3-isopropylmalate dehydratase protein )

HSP 1 Score: 158.3 bits (399), Expect = 6.7e-39
Identity = 98/195 (50.26%), Postives = 120/195 (61.54%), Query Frame = 0

Query: 13  ATSTPRSSPSISH--LSPPRTIKFPTFYSNFLNDLSTTVSH------APSRAASILPRAT 72
           A S   ++P++S    SP +   F TF S FL   ST+V+       +   ++S + R+ 
Sbjct: 2   AASLQSANPTLSRTLASPNKPSSFATFRSPFLRFNSTSVASNFKPLVSREASSSFVTRSA 61

Query: 73  ATPSSAASSSTVFHGFCYVVGDNIDTDQIIPAEYLTLVPSNPSEYEKLGSHALVGLPSSY 132
           A P    +    FHG CYVVGDNIDTDQIIPAE+LTLVPSNP EYEKLGS+ALVGLP+SY
Sbjct: 62  AEPQERKT----FHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVGLPASY 121

Query: 133 STRFVESNEMKTKYSIIIAGDNFGCGSSREHAPVALGASGAVAVVAESYARIFFRNSVAT 192
             RFV+                              GA+GA AVVA+SYARIFFRNSVAT
Sbjct: 122 KERFVQP-----------------------------GAAGAKAVVAQSYARIFFRNSVAT 163

Query: 193 GEIYPLESEIRVCDE 200
           GE+YPL+SE+RVCDE
Sbjct: 182 GEVYPLDSEVRVCDE 163