Cp4.1LG13g02310 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG13g02310
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG13: 1827937 .. 1830986 (+)
RNA-Seq Expression	Cp4.1LG13g02310
Synteny	Cp4.1LG13g02310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTCTCCCTTAACTCATTCTTCTTCTTCTTCATTTCCGTTCCGGCTTTCTTTCTCCTAGCCGCCGCCAAGCACCGCCGCCACCACTGGGTCGGACCCACCGGCCACCGCCTTATCAAAGTGGACGTCAACGGCATCTCCGGCGAGTTCCTCTCCGTCCAAGCCGCCGTGGATGCAGTCCCCGATTACAATACCGTCAACACCCTCATCCGAATCAGCCCTGGTTATTACGTGTAATGCCTCTTCTTTCCTTCACAACAAACCCAAACTTCATTATAAAACACTCATTATTTTCCATTTCATTTCTTTTCTTTTCTTCCCCTTTACGTATAAATCCTTCCCTCTTTTTTTTACTAACTTTCCCCACACGCCTTTACCTATCAAATCTTTCTAACCCCATAACCAATATCTTCATCCAATAATTACGTCTATGTCGTCGTCTTATTTAACTTGAGAAACAATATCTTGATCAATTGAGCTAGACCCGTGTCGTCGAGTTATATAACTCAATAGGTGATGTCTTGAAAAATTGAGCTAGACCCGTGTCGTTGAGTTATATAACTCGGTAGGCGATGTCTTGAAAAATTGAGCTAGACCCACGTCGTTGAGTTATATAACTCAATAGGTAATGTCTTGAAAAATTGATCTAGACTCGTGTCGTTGAGTTATATAACTCGGTAGGCGATGTCTTGAAAATTTGAGCTAGACCCATGTCGTTGAGTTATATAGCTCAATAGGTGGTGTCTTGAAAATTTGAGCAAGACCCATGTGGTTGAGTTATATAACTCGGTAGGCGATTCTTGATCAATTGAGCTAGACCCAGGTCGTCGAGTTATATAACTCGGTAGACGATGTTTTGATCAGTTGAGCTAGACTTGGGTCGTCGAGTTATATAACTCTAGACGATGTCTTGATCAATTGAGCTAACCCATGTCGTCAAATCATATAATTCGATAGATGTCTTGATCAATTGAGCTAAACCCGTTTCGTCGAGTTATATAACTCTATAGGTGATGTTTTGATCAATTGAGTTAGACCATGTTGTCGAGTTTTATAACCCAGTAGGTGATGTCTTGATTAATTGAGCTAGACCCGTGCTAGGAATTATACAATAAACTCAAAAGCTTTTAAGTTTGAGGGATTATAGGTATTATTAAGGCGTTTTGATTAAGGTTTTTTTTGGGAATGGAAATTTGCAGGGAGAAGGTGGTGGTGCCGGCGACGAAGCCGTACATAACATTTGAAGGAGGGGGAAAAGAGACGACGGTGATAGAGTGGCACGACCGAGCAGGTGACCGTGGTCCCAGCGGGCAGCAGCTGCGTACTTATAGAACAGCATCTGTCACAGTTTTTGCTAATTACTTCTCTGCCCGAAACATCAGCTTCAAGGTAAAAATAAATACCCCACCAACACTTTCCACTTCCCTGCCCGTGAACTTACTTGTCCTACATATTTCTACCATTTATTGAAATAATTATTTAATGGGTATTTCATATGTGGTTCACGTGACGGCAGAATACGGCACCGGCGCCGATGCCCGGAATGCAAGGGTGGCAGGCGGCGGCGTTTCGTATTTCCGGTGACAAGGCCTACTTTTCCGGGTGTGGATTCTACGGCGCACAAGACACCCTCTGTGACGACGCCGGCCGCCATTACTTCAAAGAATGCTACATCGAAGGCTCCATAGACTTCATTTTTGGCAACGGGCGTTCCATGTATAAGGTAATCTAATTTTAATTCCATTTCTTAATTTTTTTTAATTTCATGTATAATTTAAGGCTTTTAAAATTATAACAAAATCATTAAACGGCACCGTTTGATGCCTTAGGATTGCGAGCTGCACTCGATCGCCACCCGGTTCGGTTCCATAGCGGCCCAAGATAGAAACTCACCTAACGAGAAGACCGGGTTCGCTTTCGTCCGATGCAAGGTGACCGGTTCTGGACCTATATACGTGGGCCGGGCTATGGGCCAGTACTCCAGAATCGTCTACGCGTATACATACTTTGATGACGTGGTGGCCCACGGTGGATGGGATGACTGGGACCACAGCAGCAACAAAAACAAGTAAAATCTCTTCTAATCTCTATTGTGTGCCCTAACGGCCCATGATACCACTTCTCTTCTCTAATATGGGCTGGGCTGGGCTTACCCATCGATCTATGATCTTGGGGCCCAGTCAGTCAAATTGGGCTTATACAGGCCCAATTTGGCAATTTTGAAAGTTTATTGTTTATTCATACTTACTTTTGGGGTGGTGCAGGACTGTATTTTTTGGAGTATACAAGTGCTGGGGCCCAGGAGCAAGTAAAGTAAAGGGAGTATCATGGGCCAAGGAATTGGAGTTTGAAGAGGCCCATCCATTTCTTGTGAAGAGTTTTGTGAATGGCAGGCACTGGATTGCACCCTCTGATGCGTAGGCAGGCACCTCTGCAATGCAATGCAATGCTTCATATATCATAATATGTATTTTTCATATTTAAATTCATTTCCAAATATTTATATTATTTAATTTATTCAAATTGTACTCCAGAATTTTAAAAAAAATAGCTAATTCATCATTTAAACTTCATTTACTTTAGGCTAAATCTCAGCTGAGAAACGGTTTTCGGCTCGAGTTGCCTGTTAAAAAACAGACAAACGGGTAGTACATGTAGTTAATATGAATACAAAACAAGGTACGGAACAAAGCAGATGCTCTCCTGATACGAAAACGCCATTTCCGAATGGCGGAGGACAGCATCAAAGCACCTATCCCGAACGAGGAAAATGAAGTACGAAATTAAATAACAGTGTTACTGTTGTATAGTACACAGCAACAGTATGATGTCGATATCATCCTTCCCAAGTATAGTCGAGCATATAAAGTACATACCACAATCTTCCTCCTGCTGCCTTTGTGCACTGATGACCTACCACTACCGACGACAATGGAGATGCAGAGGACCACCAAGCTCATGTTTGCCTAACCAGTGACGAGGTAGTCAACCATAAAGGCCAGCCTAAAATAGCACTTTCGTGTTTCGAGAGGTATGCGTCGTTTAAA

mRNA sequence

ATGGCATTCTCCCTTAACTCATTCTTCTTCTTCTTCATTTCCGTTCCGGCTTTCTTTCTCCTAGCCGCCGCCAAGCACCGCCGCCACCACTGGGTCGGACCCACCGGCCACCGCCTTATCAAAGTGGACGTCAACGGCATCTCCGGCGAGTTCCTCTCCGTCCAAGCCGCCGTGGATGCAGTCCCCGATTACAATACCGTCAACACCCTCATCCGAATCAGCCCTGGTTATTACGTGGAGAAGGTGGTGGTGCCGGCGACGAAGCCGTACATAACATTTGAAGGAGGGGGAAAAGAGACGACGGTGATAGAGTGGCACGACCGAGCAGGTGACCGTGGTCCCAGCGGGCAGCAGCTGCGTACTTATAGAACAGCATCTGTCACAGTTTTTGCTAATTACTTCTCTGCCCGAAACATCAGCTTCAAGGATTGCGAGCTGCACTCGATCGCCACCCGGTTCGGTTCCATAGCGGCCCAAGATAGAAACTCACCTAACGAGAAGACCGGGTTCGCTTTCGTCCGATGCAAGGTGACCGGTTCTGGACCTATATACGTGGGCCGGGCTATGGGCCAGTACTCCAGAATCGTCTACGCGTATACATACTTTGATGACGTGGTGGCCCACGGTGGATGGGATGACTGGGACCACAGCAGCAACAAAAACAAGACTGTATTTTTTGGAGTATACAAGTGCTGGGGCCCAGGAGCAAGTAAAGTAAAGGGAGTATCATGGGCCAAGGAATTGGAGTTTGAAGAGGCCCATCCATTTCTTGTGAAGAGTTTTGTGAATGGCAGGCACTGGATTGCACCCTCTGATGCGTAGGCAGGCACCTCTGCAATGCAATGCAATGCTTCATATATCATAATATGTATTTTTCATATTTAAATTCATTTCCAAATATTTATATTATTTAATTTATTCAAATTGTACTCCAGAATTTTAAAAAAAATAGCTAATTCATCATTTAAACTTCATTTACTTTAGGCTAAATCTCAGCTGAGAAACGGTTTTCGGCTCGAGTTGCCTGTTAAAAAACAGACAAACGGGTAGTACATGTAGTTAATATGAATACAAAACAAGGTACGGAACAAAGCAGATGCTCTCCTGATACGAAAACGCCATTTCCGAATGGCGGAGGACAGCATCAAAGCACCTATCCCGAACGAGGAAAATGAAGTACGAAATTAAATAACAGTGTTACTGTTGTATAGTACACAGCAACAGTATGATGTCGATATCATCCTTCCCAAGTATAGTCGAGCATATAAAGTACATACCACAATCTTCCTCCTGCTGCCTTTGTGCACTGATGACCTACCACTACCGACGACAATGGAGATGCAGAGGACCACCAAGCTCATGTTTGCCTAACCAGTGACGAGGTAGTCAACCATAAAGGCCAGCCTAAAATAGCACTTTCGTGTTTCGAGAGGTATGCGTCGTTTAAA

Coding sequence (CDS)

ATGGCATTCTCCCTTAACTCATTCTTCTTCTTCTTCATTTCCGTTCCGGCTTTCTTTCTCCTAGCCGCCGCCAAGCACCGCCGCCACCACTGGGTCGGACCCACCGGCCACCGCCTTATCAAAGTGGACGTCAACGGCATCTCCGGCGAGTTCCTCTCCGTCCAAGCCGCCGTGGATGCAGTCCCCGATTACAATACCGTCAACACCCTCATCCGAATCAGCCCTGGTTATTACGTGGAGAAGGTGGTGGTGCCGGCGACGAAGCCGTACATAACATTTGAAGGAGGGGGAAAAGAGACGACGGTGATAGAGTGGCACGACCGAGCAGGTGACCGTGGTCCCAGCGGGCAGCAGCTGCGTACTTATAGAACAGCATCTGTCACAGTTTTTGCTAATTACTTCTCTGCCCGAAACATCAGCTTCAAGGATTGCGAGCTGCACTCGATCGCCACCCGGTTCGGTTCCATAGCGGCCCAAGATAGAAACTCACCTAACGAGAAGACCGGGTTCGCTTTCGTCCGATGCAAGGTGACCGGTTCTGGACCTATATACGTGGGCCGGGCTATGGGCCAGTACTCCAGAATCGTCTACGCGTATACATACTTTGATGACGTGGTGGCCCACGGTGGATGGGATGACTGGGACCACAGCAGCAACAAAAACAAGACTGTATTTTTTGGAGTATACAAGTGCTGGGGCCCAGGAGCAAGTAAAGTAAAGGGAGTATCATGGGCCAAGGAATTGGAGTTTGAAGAGGCCCATCCATTTCTTGTGAAGAGTTTTGTGAATGGCAGGCACTGGATTGCACCCTCTGATGCGTAG

Protein sequence

MAFSLNSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISFKDCELHSIATRFGSIAAQDRNSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPGASKVKGVSWAKELEFEEAHPFLVKSFVNGRHWIAPSDA

Homology

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 7.4e-99
Identity = 177/317 (55.84%), Postives = 208/317 (65.62%), Query Frame = 0


Query: 26  HRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVP 85
           H    WVGP+GH++I V +NG   +F SVQ AVD++P  N  +  I+I+PG+Y EKVVVP
Sbjct: 47  HHHRKWVGPSGHKVITVSLNG-HAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVP 106

Query: 86  ATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISF---- 145
           ATKPYITF+G G++ T IEWHDRA D G +GQQLRTY+TASVTV+ANYF+ARNISF    
Sbjct: 107 ATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTA 166

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 167 PAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGN 226

Query: 206 -----KDCELHSIATRFGSIAAQDRNSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIV 265
                KDCELHSIA+RFGSIAA  R  P EKTGFAFV C+VTG+GP+YVGRAMGQYSRIV
Sbjct: 227 GRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIV 286

Query: 266 YAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPGASKVKGVSWAKELEFEEAHPF 274
           YAYTYFD +VAHGGWDDWDH SNK+KT FFGVY C+GPGA+  +GVSWA+ L++E AHPF
Sbjct: 287 YAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPF 346

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 4.5e-48
Identity = 111/301 (36.88%), Postives = 150/301 (49.83%), Query Frame = 0


Query: 38  RLIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGG 97
           R+I VD NG  G+ ++VQ AVD VPD N+    I I PG Y EKV+VP +KPYI+F G  
Sbjct: 80  RVIVVDKNG-GGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNE 139

Query: 98  KET--TVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISF-------------- 157
                TVI W D+A D G  G++L TYRTASV++ +++F A  I+F              
Sbjct: 140 SYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQ 199

Query: 158 ------------------------------------------------------KDCELH 217
                                                                 +DC++H
Sbjct: 200 AVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIH 259

Query: 218 SIATRFGSIAAQDRNSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVA 269
           S A R+G+IAA  R+S  E TGF+FV C ++G+G IY+GRA G YSR VY+  +  D++ 
Sbjct: 260 STAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIIT 319

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.2e-46
Identity = 101/289 (34.95%), Postives = 146/289 (50.52%), Query Frame = 0


Query: 49  GEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDR 108
           G+F  +Q A+D++P  N V  +I++  G Y EKV +P  K +IT EG G E T +EW D 
Sbjct: 94  GDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDT 153

Query: 109 AGDRGPSGQQLRTYRTASVTVFANYFSARNISFKD------------------------- 168
           A      G  + TY +AS  V + +F A+NI+F++                         
Sbjct: 154 AQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAA 213

Query: 169 --------------------------------------------CELHSIATRFGSIAAQ 228
                                                       C +H+IA + G++ AQ
Sbjct: 214 FFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQ 273

Query: 229 DRNSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSN 269
            R+S  E TGF+FV+CKVTG+G +Y+GRA G +SR+V+AYTY D+++   GW +W   S 
Sbjct: 274 GRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPS- 333

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.8e-42
Identity = 99/288 (34.38%), Postives = 149/288 (51.74%), Query Frame = 0


Query: 39  LIKVDVNGISGEFLSVQAAVDAVPD--YNTVNTLIRISPGYYVEKVVVPATKPYITFEGG 98
           LI+VD +G  G+F  +Q A++++P    N+    I + PG Y EKVV+PA KPYIT  G 
Sbjct: 50  LIRVDQSG-KGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGT 109

Query: 99  GKETTVIEWHD--------------------------RAGDRGPS-------------GQ 158
               T + W D                          + G  G +             G 
Sbjct: 110 QASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKAAFYGC 169

Query: 159 QLRTYRTASVTVFANYF--------------SARNISFKDCELHSIATRFGSIAAQDRNS 218
            + +Y+   +    N++               + +  ++ C LHS++   GSI AQ R S
Sbjct: 170 VITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITAQMRTS 229

Query: 219 PNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKT 272
             EK+GF F+ CK+TGSG  ++GR  G YSR+V+AY++F +VVA  GW+ W  S+ +N T
Sbjct: 230 ATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKEN-T 289

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.0e-36
Identity = 105/330 (31.82%), Postives = 148/330 (44.85%), Query Frame = 0


Query: 26  HRRHH--------WVGPTGHR-----LIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIR 85
           H  HH        W     H+     ++ VD++G  G F +VQ+A+D VPD ++  TLI 
Sbjct: 64  HYHHHEPIKCCEKWTSRLRHQYKTSLVLTVDLHG-CGNFSNVQSAIDVVPDLSSSKTLII 123

Query: 86  ISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFAN 145
           ++ G Y EKV V   K  +  +G G + T IEW+D A   G       T  + S  VFA 
Sbjct: 124 VNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAA 183

Query: 146 YFSARNISFK-------------------------------------------------- 205
            F+A NISFK                                                  
Sbjct: 184 NFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKE 243

Query: 206 -------------------DCELHSIATR-----FGSIAAQDRNSPNEKTGFAFVRCKVT 265
                              DC ++SIA        GSI AQ R S +E++GF+FV CK+ 
Sbjct: 244 CFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKID 303

Query: 266 GSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPGASK 269
           GSG I +GRA G Y+ +V++ TY   ++   GW++W   S K KTV FG +KC+GPGA  
Sbjct: 304 GSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWG-DSTKEKTVTFGEHKCYGPGADY 363

[Close]

BLAST of Cp4.1LG13g02310 vs. NCBI nr
Match: XP_023550838.1 (probable pectinesterase 68 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532 bits (1371), Expect = 2.27e-189
Identity = 273/342 (79.82%), Postives = 273/342 (79.82%), Query Frame = 0


Query: 1   MAFSLNSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDA 60
           MAFSLNSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDA
Sbjct: 1   MAFSLNSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDA 60

Query: 61  VPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR 120
           VPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR
Sbjct: 61  VPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR 120

Query: 121 TYRTASVTVFANYFSARNISFK-------------------------------------- 180
           TYRTASVTVFANYFSARNISFK                                      
Sbjct: 121 TYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDT 180

Query: 181 -------------------------------DCELHSIATRFGSIAAQDRNSPNEKTGFA 240
                                          DCELHSIATRFGSIAAQDRNSPNEKTGFA
Sbjct: 181 LCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPNEKTGFA 240

Query: 241 FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKC 273
           FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKC
Sbjct: 241 FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKC 300

[Close]

BLAST of Cp4.1LG13g02310 vs. NCBI nr
Match: KAG6579412.1 (putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 524 bits (1350), Expect = 3.72e-186
Identity = 270/343 (78.72%), Postives = 272/343 (79.30%), Query Frame = 0


Query: 1   MAFSLNSFFFFF-ISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60
           MAFSLNSFFFFF ISVPAFFLLAAAKH RHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD
Sbjct: 1   MAFSLNSFFFFFFISVPAFFLLAAAKHHRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60

Query: 61  AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120
           A+PDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL
Sbjct: 61  AIPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120

Query: 121 RTYRTASVTVFANYFSARNISFK------------------------------------- 180
           RTYRTASVTVFANYFSARNISFK                                     
Sbjct: 121 RTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQD 180

Query: 181 --------------------------------DCELHSIATRFGSIAAQDRNSPNEKTGF 240
                                           DCELHSIATRFGSIAAQDRNSPNEKTGF
Sbjct: 181 TLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPNEKTGF 240

Query: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYK 273
           AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH+SNKNKTVFFGVYK
Sbjct: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKNKTVFFGVYK 300

[Close]

BLAST of Cp4.1LG13g02310 vs. NCBI nr
Match: XP_022922190.1 (probable pectinesterase 68 [Cucurbita moschata])

HSP 1 Score: 523 bits (1347), Expect = 1.07e-185
Identity = 270/343 (78.72%), Postives = 271/343 (79.01%), Query Frame = 0


Query: 1   MAFSLNSFFFFF-ISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60
           M FSLNSFFFFF ISVPAFFLLAAAKH RHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD
Sbjct: 1   MTFSLNSFFFFFFISVPAFFLLAAAKHHRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60

Query: 61  AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120
           AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL
Sbjct: 61  AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120

Query: 121 RTYRTASVTVFANYFSARNISFK------------------------------------- 180
           RTYRTASVTVFANYFSARNISFK                                     
Sbjct: 121 RTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQD 180

Query: 181 --------------------------------DCELHSIATRFGSIAAQDRNSPNEKTGF 240
                                           DCELHSIATRFGSIAAQDRNSPNEKTGF
Sbjct: 181 TLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPNEKTGF 240

Query: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYK 273
           AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH+SNKNKTVFFGVYK
Sbjct: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKNKTVFFGVYK 300

[Close]

BLAST of Cp4.1LG13g02310 vs. NCBI nr
Match: KAG7016889.1 (putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522 bits (1344), Expect = 3.05e-185
Identity = 269/343 (78.43%), Postives = 271/343 (79.01%), Query Frame = 0


Query: 1   MAFSLNSFFFFF-ISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60
           MAFSLNSFFFFF ISVPAFFLLAAAKH RHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD
Sbjct: 1   MAFSLNSFFFFFFISVPAFFLLAAAKHHRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60

Query: 61  AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120
           A+PDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL
Sbjct: 61  AIPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120

Query: 121 RTYRTASVTVFANYFSARNISFK------------------------------------- 180
           RTYRTASVTVFANYFSARNISFK                                     
Sbjct: 121 RTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQD 180

Query: 181 --------------------------------DCELHSIATRFGSIAAQDRNSPNEKTGF 240
                                           DCELHSIATRFGSIAAQDRN PNEKTGF
Sbjct: 181 TLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNLPNEKTGF 240

Query: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYK 273
           AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH+SNKNKTVFFGVYK
Sbjct: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKNKTVFFGVYK 300

[Close]

BLAST of Cp4.1LG13g02310 vs. NCBI nr
Match: XP_022969900.1 (probable pectinesterase 68 [Cucurbita maxima])

HSP 1 Score: 515 bits (1326), Expect = 1.62e-182
Identity = 264/342 (77.19%), Postives = 268/342 (78.36%), Query Frame = 0


Query: 1   MAFSLNSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDA 60
           MAFSL SFFFFFISVPAFFLLAAAKH RHHWVG TGHRLIKVDV GISGEF+SVQAAVDA
Sbjct: 1   MAFSLTSFFFFFISVPAFFLLAAAKHHRHHWVGHTGHRLIKVDVKGISGEFVSVQAAVDA 60

Query: 61  VPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR 120
           VPDYNTVNTLIRISPGYYVEKVVVPA KPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR
Sbjct: 61  VPDYNTVNTLIRISPGYYVEKVVVPAKKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR 120

Query: 121 TYRTASVTVFANYFSARNISFK-------------------------------------- 180
           TYRTASVTVFANYFSARNISFK                                      
Sbjct: 121 TYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDT 180

Query: 181 -------------------------------DCELHSIATRFGSIAAQDRNSPNEKTGFA 240
                                          DCELHSIATRFGSIAAQDRNSPNEKTGFA
Sbjct: 181 LCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPNEKTGFA 240

Query: 241 FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKC 273
           FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH+SNKN+TVFFGVYKC
Sbjct: 241 FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKNQTVFFGVYKC 300

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy TrEMBL
Match: A0A6J1E808 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430254 PE=4 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 5.16e-186
Identity = 270/343 (78.72%), Postives = 271/343 (79.01%), Query Frame = 0


Query: 1   MAFSLNSFFFFF-ISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60
           M FSLNSFFFFF ISVPAFFLLAAAKH RHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD
Sbjct: 1   MTFSLNSFFFFFFISVPAFFLLAAAKHHRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVD 60

Query: 61  AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120
           AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL
Sbjct: 61  AVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQL 120

Query: 121 RTYRTASVTVFANYFSARNISFK------------------------------------- 180
           RTYRTASVTVFANYFSARNISFK                                     
Sbjct: 121 RTYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQD 180

Query: 181 --------------------------------DCELHSIATRFGSIAAQDRNSPNEKTGF 240
                                           DCELHSIATRFGSIAAQDRNSPNEKTGF
Sbjct: 181 TLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPNEKTGF 240

Query: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYK 273
           AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH+SNKNKTVFFGVYK
Sbjct: 241 AFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKNKTVFFGVYK 300

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy TrEMBL
Match: A0A6J1I193 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468960 PE=4 SV=1)

HSP 1 Score: 515 bits (1326), Expect = 7.87e-183
Identity = 264/342 (77.19%), Postives = 268/342 (78.36%), Query Frame = 0


Query: 1   MAFSLNSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDA 60
           MAFSL SFFFFFISVPAFFLLAAAKH RHHWVG TGHRLIKVDV GISGEF+SVQAAVDA
Sbjct: 1   MAFSLTSFFFFFISVPAFFLLAAAKHHRHHWVGHTGHRLIKVDVKGISGEFVSVQAAVDA 60

Query: 61  VPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR 120
           VPDYNTVNTLIRISPGYYVEKVVVPA KPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR
Sbjct: 61  VPDYNTVNTLIRISPGYYVEKVVVPAKKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLR 120

Query: 121 TYRTASVTVFANYFSARNISFK-------------------------------------- 180
           TYRTASVTVFANYFSARNISFK                                      
Sbjct: 121 TYRTASVTVFANYFSARNISFKNTAPAPMPGMQGWQAAAFRISGDKAYFSGCGFYGAQDT 180

Query: 181 -------------------------------DCELHSIATRFGSIAAQDRNSPNEKTGFA 240
                                          DCELHSIATRFGSIAAQDRNSPNEKTGFA
Sbjct: 181 LCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPNEKTGFA 240

Query: 241 FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKC 273
           FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH+SNKN+TVFFGVYKC
Sbjct: 241 FVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHTSNKNQTVFFGVYKC 300

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy TrEMBL
Match: A0A5A7T9Y3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00500 PE=4 SV=1)

HSP 1 Score: 468 bits (1205), Expect = 2.02e-164
Identity = 241/337 (71.51%), Postives = 253/337 (75.07%), Query Frame = 0


Query: 6   NSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDAVPDYN 65
           +SFFFF   +  FF  +AA +  HHWVGPTGHRLIKV+VNG S  FLSVQAAVD++PDYN
Sbjct: 7   HSFFFFTFFLLCFF--SAAANHLHHWVGPTGHRLIKVNVNGGSDVFLSVQAAVDSIPDYN 66

Query: 66  TVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTA 125
           TVNT+IRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTA
Sbjct: 67  TVNTIIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTA 126

Query: 126 SVTVFANYFSARNISFK------------------------------------------- 185
           SVTVFANYFSARNISFK                                           
Sbjct: 127 SVTVFANYFSARNISFKNTAPAPLPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDA 186

Query: 186 --------------------------DCELHSIATRFGSIAAQDRNSPNEKTGFAFVRCK 245
                                     DCELHSIATRFGSIAAQDRNSP+EKTGFAF+RCK
Sbjct: 187 GRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPHEKTGFAFLRCK 246

Query: 246 VTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPGA 273
           VTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKNKTVFFGVYKCWGPGA
Sbjct: 247 VTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYKCWGPGA 306

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy TrEMBL
Match: A0A1S3AUB3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483059 PE=4 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 7.26e-163
Identity = 238/329 (72.34%), Postives = 248/329 (75.38%), Query Frame = 0


Query: 14  SVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIRI 73
           S  AFF  +AA +  HHWVGPTGHRLIKV+VNG S  FLSVQAAVD++PDYNTVNT+IRI
Sbjct: 27  SSSAFF--SAATNHLHHWVGPTGHRLIKVNVNGGSDVFLSVQAAVDSIPDYNTVNTIIRI 86

Query: 74  SPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANY 133
           SPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANY
Sbjct: 87  SPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANY 146

Query: 134 FSARNISFK--------------------------------------------------- 193
           FSARNISFK                                                   
Sbjct: 147 FSARNISFKNTAPAPLPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDDAGRHYFKEC 206

Query: 194 ------------------DCELHSIATRFGSIAAQDRNSPNEKTGFAFVRCKVTGSGPIY 253
                             DCELHSIATRFGSIAAQDRNSP+EKTGFAF+RCKVTGSGPIY
Sbjct: 207 YIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPHEKTGFAFLRCKVTGSGPIY 266

Query: 254 VGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPGASKVKGVSW 273
           VGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKNKTVFFGVYKCWGPGASKVKGVSW
Sbjct: 267 VGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYKCWGPGASKVKGVSW 326

[Close]

BLAST of Cp4.1LG13g02310 vs. ExPASy TrEMBL
Match: A0A0A0KP96 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G162010 PE=4 SV=1)

HSP 1 Score: 464 bits (1193), Expect = 1.22e-162
Identity = 239/338 (70.71%), Postives = 249/338 (73.67%), Query Frame = 0


Query: 5   LNSFFFFFISVPAFFLLAAAKHRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDAVPDY 64
           L+SFFF       FFLL  +    HHW+GPTGHRLIKV+V G S  FLSVQAAVD+VPDY
Sbjct: 6   LHSFFF-----STFFLLCFSAAANHHWIGPTGHRLIKVNVKGGSDVFLSVQAAVDSVPDY 65

Query: 65  NTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRT 124
           NTVNT+IRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRT
Sbjct: 66  NTVNTIIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRT 125

Query: 125 ASVTVFANYFSARNISFK------------------------------------------ 184
           ASVTVFANYFSARNISFK                                          
Sbjct: 126 ASVTVFANYFSARNISFKNTAPAPLPGMQGWQAAAFRISGDKAYFSGCGFYGAQDTLCDD 185

Query: 185 ---------------------------DCELHSIATRFGSIAAQDRNSPNEKTGFAFVRC 244
                                      DCELHSIATRFGSIAAQDRNSP EKTGFAF+RC
Sbjct: 186 AGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIATRFGSIAAQDRNSPIEKTGFAFLRC 245

Query: 245 KVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPG 273
           KVTGSGP+YVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDH SNKNKTVFFGVYKCWGPG
Sbjct: 246 KVTGSGPVYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHVSNKNKTVFFGVYKCWGPG 305

[Close]

BLAST of Cp4.1LG13g02310 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 5.2e-100
Identity = 177/317 (55.84%), Postives = 208/317 (65.62%), Query Frame = 0


Query: 26  HRRHHWVGPTGHRLIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVP 85
           H    WVGP+GH++I V +NG   +F SVQ AVD++P  N  +  I+I+PG+Y EKVVVP
Sbjct: 47  HHHRKWVGPSGHKVITVSLNG-HAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVP 106

Query: 86  ATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISF---- 145
           ATKPYITF+G G++ T IEWHDRA D G +GQQLRTY+TASVTV+ANYF+ARNISF    
Sbjct: 107 ATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTA 166

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 167 PAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGN 226

Query: 206 -----KDCELHSIATRFGSIAAQDRNSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIV 265
                KDCELHSIA+RFGSIAA  R  P EKTGFAFV C+VTG+GP+YVGRAMGQYSRIV
Sbjct: 227 GRSMYKDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIV 286

Query: 266 YAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPGASKVKGVSWAKELEFEEAHPF 274
           YAYTYFD +VAHGGWDDWDH SNK+KT FFGVY C+GPGA+  +GVSWA+ L++E AHPF
Sbjct: 287 YAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPF 346

[Close]

BLAST of Cp4.1LG13g02310 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 193.0 bits (489), Expect = 3.2e-49
Identity = 111/301 (36.88%), Postives = 150/301 (49.83%), Query Frame = 0


Query: 38  RLIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGG 97
           R+I VD NG  G+ ++VQ AVD VPD N+    I I PG Y EKV+VP +KPYI+F G  
Sbjct: 80  RVIVVDKNG-GGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNE 139

Query: 98  KET--TVIEWHDRAGDRGPSGQQLRTYRTASVTVFANYFSARNISF-------------- 157
                TVI W D+A D G  G++L TYRTASV++ +++F A  I+F              
Sbjct: 140 SYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQ 199

Query: 158 ------------------------------------------------------KDCELH 217
                                                                 +DC++H
Sbjct: 200 AVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIH 259

Query: 218 SIATRFGSIAAQDRNSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVA 269
           S A R+G+IAA  R+S  E TGF+FV C ++G+G IY+GRA G YSR VY+  +  D++ 
Sbjct: 260 STAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIIT 319

[Close]

BLAST of Cp4.1LG13g02310 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.3e-47
Identity = 101/289 (34.95%), Postives = 146/289 (50.52%), Query Frame = 0


Query: 49  GEFLSVQAAVDAVPDYNTVNTLIRISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDR 108
           G+F  +Q A+D++P  N V  +I++  G Y EKV +P  K +IT EG G E T +EW D 
Sbjct: 94  GDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDT 153

Query: 109 AGDRGPSGQQLRTYRTASVTVFANYFSARNISFKD------------------------- 168
           A      G  + TY +AS  V + +F A+NI+F++                         
Sbjct: 154 AQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAA 213

Query: 169 --------------------------------------------CELHSIATRFGSIAAQ 228
                                                       C +H+IA + G++ AQ
Sbjct: 214 FFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQ 273

Query: 229 DRNSPNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSN 269
            R+S  E TGF+FV+CKVTG+G +Y+GRA G +SR+V+AYTY D+++   GW +W   S 
Sbjct: 274 GRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPS- 333

[Close]

BLAST of Cp4.1LG13g02310 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 173.7 bits (439), Expect = 2.0e-43
Identity = 99/288 (34.38%), Postives = 149/288 (51.74%), Query Frame = 0


Query: 39  LIKVDVNGISGEFLSVQAAVDAVPD--YNTVNTLIRISPGYYVEKVVVPATKPYITFEGG 98
           LI+VD +G  G+F  +Q A++++P    N+    I + PG Y EKVV+PA KPYIT  G 
Sbjct: 50  LIRVDQSG-KGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGT 109

Query: 99  GKETTVIEWHD--------------------------RAGDRGPS-------------GQ 158
               T + W D                          + G  G +             G 
Sbjct: 110 QASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKAAFYGC 169

Query: 159 QLRTYRTASVTVFANYF--------------SARNISFKDCELHSIATRFGSIAAQDRNS 218
            + +Y+   +    N++               + +  ++ C LHS++   GSI AQ R S
Sbjct: 170 VITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITAQMRTS 229

Query: 219 PNEKTGFAFVRCKVTGSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKT 272
             EK+GF F+ CK+TGSG  ++GR  G YSR+V+AY++F +VVA  GW+ W  S+ +N T
Sbjct: 230 ATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKEN-T 289

[Close]

BLAST of Cp4.1LG13g02310 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 7.4e-38
Identity = 105/330 (31.82%), Postives = 148/330 (44.85%), Query Frame = 0


Query: 26  HRRHH--------WVGPTGHR-----LIKVDVNGISGEFLSVQAAVDAVPDYNTVNTLIR 85
           H  HH        W     H+     ++ VD++G  G F +VQ+A+D VPD ++  TLI 
Sbjct: 64  HYHHHEPIKCCEKWTSRLRHQYKTSLVLTVDLHG-CGNFSNVQSAIDVVPDLSSSKTLII 123

Query: 86  ISPGYYVEKVVVPATKPYITFEGGGKETTVIEWHDRAGDRGPSGQQLRTYRTASVTVFAN 145
           ++ G Y EKV V   K  +  +G G + T IEW+D A   G       T  + S  VFA 
Sbjct: 124 VNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAA 183

Query: 146 YFSARNISFK-------------------------------------------------- 205
            F+A NISFK                                                  
Sbjct: 184 NFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKE 243

Query: 206 -------------------DCELHSIATR-----FGSIAAQDRNSPNEKTGFAFVRCKVT 265
                              DC ++SIA        GSI AQ R S +E++GF+FV CK+ 
Sbjct: 244 CFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKID 303

Query: 266 GSGPIYVGRAMGQYSRIVYAYTYFDDVVAHGGWDDWDHSSNKNKTVFFGVYKCWGPGASK 269
           GSG I +GRA G Y+ +V++ TY   ++   GW++W   S K KTV FG +KC+GPGA  
Sbjct: 304 GSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWG-DSTKEKTVTFGEHKCYGPGADY 363

The following BLAST results are available for this feature:

BLAST of Cp4.1LG13g02310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPF3	7.4e-99	55.84	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9FM79	4.5e-48	36.88	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q8VYZ3	3.2e-46	34.95	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9SIJ9	2.8e-42	34.38	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q9ZQA3	1.0e-36	31.82	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]

BLAST of Cp4.1LG13g02310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023550838.1	2.27e-189	79.82	probable pectinesterase 68 [Cucurbita pepo subsp. pepo]	[more]
KAG6579412.1	3.72e-186	78.72	putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922190.1	1.07e-185	78.72	probable pectinesterase 68 [Cucurbita moschata]	[more]
KAG7016889.1	3.05e-185	78.43	putative pectinesterase 68, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022969900.1	1.62e-182	77.19	probable pectinesterase 68 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG13g02310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E808	5.16e-186	78.72	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430254 PE=4 SV=1	[more]
A0A6J1I193	7.87e-183	77.19	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468960 PE=4 SV=1	[more]
A0A5A7T9Y3	2.02e-164	71.51	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00500...	[more]
A0A1S3AUB3	7.26e-163	72.34	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483059 PE=4 SV=1	[more]
A0A0A0KP96	1.22e-162	70.71	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G162010 PE=4 SV=1	[more]

BLAST of Cp4.1LG13g02310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47500.1	5.2e-100	55.84	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	3.2e-49	36.88	Pectin lyase-like superfamily protein	[more]
AT5G19730.1	2.3e-47	34.95	Pectin lyase-like superfamily protein	[more]
AT2G21610.1	2.0e-43	34.38	pectinesterase 11	[more]
AT2G36710.1	7.4e-38	31.82	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 39..141 e-value: 3.2E-24 score: 88.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 142..272 e-value: 1.9E-33 score: 118.1
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 48..143 e-value: 2.0E-14 score: 53.0 coord: 151..264 e-value: 2.5E-18 score: 65.8
None	No IPR available	PANTHER	PTHR31321:SF19	PECTINESTERASE 68-RELATED	coord: 23..142 coord: 141..272
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 23..142 coord: 141..272
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 38..270

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG13g02310.1	Cp4.1LG13g02310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity