Cp4.1LG12g06710 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG12g06710 vs. ExPASy Swiss-Prot
Match: Q8L709 (Cation/H(+) antiporter 28 OS=Arabidopsis thaliana OX=3702 GN=CHX28 PE=2 SV=2)

HSP 1 Score: 808.1 bits (2086), Expect = 8.6e-233
Identity = 415/775 (53.55%), Postives = 554/775 (71.48%), Query Frame = 0

Query: 33  ILGFFLMMGLCNGVHYLLRPFSQPRTTSDTIVGLVVGNFMRKELDLS--IHKTLRYIVDF 92
           IL F  +  +   +HYL++P  QP  T+D  +GL++GN  R     S     TL  I++F
Sbjct: 27  ILVFIAIFVVRTLLHYLMKPLGQPYLTTDFAIGLILGNIPRFRGAFSGPYSITLNNIIEF 86

Query: 93  GMTCYMFVLGLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSKEISFILS 152
           GM C+MFV+GLEM+P VL + PT+DA +AY  MI+T +LA  T PF+ ++K+    F L+
Sbjct: 87  GMICHMFVMGLEMNPSVLLRPPTKDAFIAYTSMITTFVLAFVTTPFLHYTKTSPYIFSLA 146

Query: 153 LSTVLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGYLFCQCQETRL 212
           LS + SST SP+LTR+I NLKI +SD+G+L  AAG+H+D ISTL  C G++F   ++   
Sbjct: 147 LSLMASSTGSPILTRVIANLKIRKSDLGKLASAAGVHTDMISTLLYCFGFIFFPTEKPLA 206

Query: 213 SIINGFQLGFVL----LIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVAFMAFLC 272
             ++ F    ++    L Q+   + VSPIF+NWVNNENPEGKP+KG HLV+++AF+  +C
Sbjct: 207 RPLHRFFRALLMFCLFLAQVTFTSIVSPIFLNWVNNENPEGKPLKGSHLVMSLAFVVLIC 266

Query: 273 CCPT---IFGYNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVESKL 332
             PT      YNP LSAF AG FLP +GR+S+W I KINYLL+TVFYPIFFFW+G    +
Sbjct: 267 SFPTWPPESMYNPILSAFTAGLFLPNKGRMSKWIINKINYLLSTVFYPIFFFWVGFIIHM 326

Query: 333 SDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHIYL 392
            +F+    + WVR   L   V  GKV GTV+ G +LG+H PE+ +LGLLLT KGHFH+YL
Sbjct: 327 RNFDITDKMAWVRFFSLLGTVIAGKVTGTVLCGLLLGYHVPETASLGLLLTTKGHFHVYL 386

Query: 393 AISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHIIRRARKRTPTHRMALELLDPSSEL 452
           A  A    ++ ++T   ++F+I FTVV++P VV  II+RARKR P H MAL+ LDP++EL
Sbjct: 387 AALAIRTNRVKSTTGALIIFIIVFTVVYSPFVVMDIIKRARKRVPVHIMALQWLDPTTEL 446

Query: 453 KILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDMIELTDEIAATLVQGEGV----DT 512
           +IL+ +HGPHN G+ +N+MEI  G   PG + Y TDM+ELTDEIAATL +G G     D+
Sbjct: 447 RILIGLHGPHNIGSTLNVMEICHGGREPGSIFYATDMVELTDEIAATLKKGGGAGQSNDS 506

Query: 513 VTVTHTGVTQMRDQVTTAFQRYVD-ENGDGITLRRMLALSAFNCMAQDICILAEDLLGAL 572
           VTVT   VT+MR+ +T A   Y +  NG G+T+RRMLALS F  MA D+C LA++L+ ++
Sbjct: 507 VTVTDRSVTEMRESITAAVNGYGELRNGQGVTVRRMLALSTFVTMAHDVCGLADELMVSI 566

Query: 573 IILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKISRSYVTLNV 632
           IILPFHK    DG+L  G AGFR+VNRK++++APCSVGILVDR  G  E+  R   ++ +
Sbjct: 567 IILPFHKRLNPDGTLDAGHAGFRHVNRKILKNAPCSVGILVDRSFGQTEEAWRPGASMGI 626

Query: 633 AVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENAARRAGTY-RISVAEQEEE 692
           A+IFIGG+DDREALA+A +VARHP VKL VIRFL D  ++NA +R+    R SV +QEEE
Sbjct: 627 AIIFIGGRDDREALAFAAQVARHPAVKLKVIRFLEDKSSQNAQKRSSILNRASVVDQEEE 686

Query: 693 MRQDDECFAYFYERHVA-GGHVAYVEKHMASSAETYSTLKSLEGQYTLVIVGRGGG-VNS 752
           M+ DDECFA FYER++A GG V+Y+EKH+ +S+ET++ LKSL+G+Y LVIVGRGGG  +S
Sbjct: 687 MKLDDECFAEFYERYIAGGGRVSYMEKHLTNSSETFTALKSLDGEYGLVIVGRGGGRASS 746

Query: 753 VLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGELDGLDDDFSIM 791
            LT G+NDWQQCPELGPIGDVLSGS+FS  TS+LII Q   +G+L+GL DDF+I+
Sbjct: 747 GLTTGLNDWQQCPELGPIGDVLSGSDFSHNTSMLIIQQQRTRGQLEGLHDDFTIL 801

BLAST of Cp4.1LG12g06710 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.3e-99
Identity = 222/745 (29.80%), Postives = 384/745 (51.54%), Query Frame = 0

Query: 48  YLLRPFSQPRTTSDTIVGLVVGNFMRKELDLSIH--------KTLRYIVDFGMTCYMFVL 107
           ++L+PF QPR  S+ + G+V+G  +        H          L  + + G+  ++F++
Sbjct: 56  FILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLV 115

Query: 108 GLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSK------EISFILSLST 167
           G+EMD  V+ K   R   +A  GM+   ++  +     SFS  +      + ++IL L  
Sbjct: 116 GVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAA----FSFSMHRSEDHLGQGTYILFLGV 175

Query: 168 VLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGYLFCQCQETRLSII 227
            LS TA PVL R++  LK+  ++IGR+ ++A + +D  + + + +     +  +T  + +
Sbjct: 176 ALSVTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASL 235

Query: 228 NGFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFG 287
                  V +   +   +     + W+  + PEG+     H+ L +  +          G
Sbjct: 236 WVMISSAVFIAVCVFVVRPG---IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIG 295

Query: 288 YNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVESKLSDFEPGQIIT 347
            +    AF+ G  +P  G +    I K+   ++ +  P+FF   G+++ ++  +     T
Sbjct: 296 THSVFGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGP--AT 355

Query: 348 WVRLLLLFAIVTVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHIYLAISAKIAGKI 407
           W+ L L+  +   GKV+GTVI     G    E + LGLLL  KG   + +    K    +
Sbjct: 356 WLTLFLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVL 415

Query: 408 STSTSIAMVFVIFFTVVHAPKVVAHIIRRARKRTPTHRMALELLDPSSELKILLCIHGPH 467
              T   MV V          +V  + +  +K     R  ++   P SEL++L+C+H P 
Sbjct: 416 DDETFATMVLVALVMTGVITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPR 475

Query: 468 NTGAAINIMEISRGTANPGVVVYVTDMIELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQ 527
           N    IN++E S  T    + +YV  ++ELT   +A L+      +            D 
Sbjct: 476 NVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDH 535

Query: 528 VTTAFQRYVDENGDGITLRRMLALSAFNCMAQDICILAEDLLGALIILPFHKNQRGDGSL 587
           +  AF+ Y +++   + ++ + A+S ++ M +D+C LAED   + II+PFHK Q  DG +
Sbjct: 536 IINAFENY-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGM 595

Query: 588 SEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKISRSYVTLNVAVIFIGGKDDREALA 647
                 +R VN+ ++ ++PCSVGILVDRGL    +++ + V+L VAV+F GG DDREALA
Sbjct: 596 ESTNPAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVSLQVAVLFFGGPDDREALA 655

Query: 648 YAGRVARHPGVKLSVIRFLVDAD-AENAARRA---GTYRISVAEQEEEMRQDDECFAYFY 707
           YA R+A+HPG+ L+V+RF+ D D A+ A+ RA      +I   +  ++ + DD+    F 
Sbjct: 656 YAWRMAQHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFR 715

Query: 708 ERHVAGGHVAYVEKHMASSAETYSTLKSLEGQYTLVIVGRGGGVNSVLTYGMNDWQQCPE 767
             +     + Y+EK +++  ET + ++S++  + L IVGRG G++S LT G+ DW +CPE
Sbjct: 716 AENAEYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPE 775

Query: 768 LGPIGDVLSGSEFSAKTSVLIIHQH 775
           LG IGD+L+ S+F+A  SVL++ Q+
Sbjct: 776 LGAIGDLLASSDFAATVSVLVVQQY 789

BLAST of Cp4.1LG12g06710 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.5e-85
Identity = 232/774 (29.97%), Postives = 387/774 (50.00%), Query Frame = 0

Query: 32  SILGFFLMMGLCNGVHYLLRPFSQPRTTSDTIVGLVVGNFM----RKELDLSIHK----T 91
           +IL   +++ L   + YLLRP  QPR  ++ I G+++G  +    +  LD    K     
Sbjct: 34  AILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTV 93

Query: 92  LRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSK 151
           L  + + G+  ++F+ GLE+D   L +   +   +A AG+     L   +   +  + SK
Sbjct: 94  LETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKATISK 153

Query: 152 EI---SFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGY 211
            +   +F++ +   LS TA PVL R++  LK+  ++IGRL ++A   +D  + + + +  
Sbjct: 154 GVNSTAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILLALAI 213

Query: 212 LFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVA 271
                  + L  +  F  G   +I    A+ + P    W++    EG+P++  ++   +A
Sbjct: 214 ALSGSNTSPLVSLWVFLSGCAFVIG---ASFIIPPIFRWISRRCHEGEPIEETYICATLA 273

Query: 272 FMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVE 331
            +          G +    AF+ G  +P+EG  +   + K+  L++ +F P++F   G++
Sbjct: 274 VVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPLYFVASGLK 333

Query: 332 SKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILGFHWP--ESVALGLLLTMKGH 391
           + ++  +  Q  +W  L+L+ A    GK++GT+  G  L F  P  E++ LG L+  KG 
Sbjct: 334 TNVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLGFLMNTKGL 393

Query: 392 FHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHI---IRRARKRTPTHRMALE 451
             + +    K    ++  T   MV +  FT      VV  +    RRA+K       A+E
Sbjct: 394 VELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVE 453

Query: 452 LLDPSSELKILLCIHGPHNTGAAINIMEISRG-TANPGVVVYVTDMIELTDEIAATL--- 511
             + +++L+IL C HG  +  + IN++E SRG     G+ VY   + EL++  +A L   
Sbjct: 454 RENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVH 513

Query: 512 -VQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAFNCMAQDICIL 571
            V+  G+        GV    DQV  AFQ +  +    + +R M A+S+ + + +DIC  
Sbjct: 514 KVRKNGMP--FWNRRGVNADADQVVVAFQAF--QQLSRVNVRPMTAISSMSDIHEDICTT 573

Query: 572 AEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKIS 631
           A     A++ILPFHK+Q+ DGSL   +  +R+VNR+V+  APCSVGI VDRGLG   ++S
Sbjct: 574 AVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRGLGGSSQVS 633

Query: 632 RSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENAARRAGTYRIS 691
              V+ +V V+F GG DDREALAY  R+A HPG+ L+V RF+V  +     R      + 
Sbjct: 634 AQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPE-----RVGEIVNVE 693

Query: 692 VAEQEEE------MRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEGQYTL 751
           V+    E      ++ D+E  +   +       V +VEK + ++A    +      +  L
Sbjct: 694 VSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVRRSNL 753

Query: 752 VIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKG 779
            +VGR  G    L    N   +CPELGP+G +L   E S K SVL+I Q+N  G
Sbjct: 754 FLVGRMPGGEIALAIREN--SECPELGPVGSLLISPESSTKASVLVIQQYNGTG 789

BLAST of Cp4.1LG12g06710 vs. ExPASy Swiss-Prot
Match: Q9LUN4 (Cation/H(+) antiporter 19 OS=Arabidopsis thaliana OX=3702 GN=CHX19 PE=2 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.8e-82
Identity = 221/759 (29.12%), Postives = 374/759 (49.28%), Query Frame = 0

Query: 33  ILGFFLMMGLCNGVHYLLRPFSQPRTTSDTIVGLVVG----NFMRKELDLSIHK----TL 92
           IL   L++     + Y L+P  QPR  ++ I G+++G       +  LD    K     L
Sbjct: 36  ILQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVL 95

Query: 93  RYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSK- 152
             + + G+  ++F++GLE+D   + K   +   +A AG+    I+   T   +S + SK 
Sbjct: 96  DTLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLPFIVGVGTSFVLSATISKG 155

Query: 153 --EISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGYL 212
             ++ FI+ +   LS TA PVL R++  LK+  +DIGR+ ++A   +D  + + + +   
Sbjct: 156 VDQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIA 215

Query: 213 FCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVAF 272
                 + L  +     G   +I  ++A K     + ++    PEG+P+K  ++ + +  
Sbjct: 216 LSGDGTSPLVSVWVLLCGTGFVIFAVVAIKP---LLAYMARRCPEGEPVKELYVCVTLTV 275

Query: 273 MAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVES 332
           +          G +    AF+ G   P+EG   R    KI  L++ +  P++F   G+++
Sbjct: 276 VLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLKT 335

Query: 333 KLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHI 392
            ++     Q  +W  L+L+      GK+VGTV +  +    + E+V LG L+  KG   +
Sbjct: 336 DVTTIRGAQ--SWGLLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVEL 395

Query: 393 YLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHIIRRARKRTPTHRMALELLDPSS 452
            +    K    ++      +V +  FT      +V  I + ARK  P     ++  D  S
Sbjct: 396 IVLNIGKDRKVLNDQAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHRTIQRKDHDS 455

Query: 453 ELKILLCIHGPHNTGAAINIMEISRGTANPG-VVVYVTDMIELTDEIAATLVQGEGVDTV 512
           EL+IL C H   N    IN++E SRGT   G + VY   ++EL++  +A  +  +  +  
Sbjct: 456 ELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARNNG 515

Query: 513 TVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAFNCMAQDICILAEDLLGALII 572
                 + +  DQ+  AF+ Y  ++   + +R M A+S  + + +DIC  A     A+I+
Sbjct: 516 LPIWNKIERSTDQMVIAFEAY--QHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAMIL 575

Query: 573 LPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKISRSYVTLNVAV 632
           LPFHK+QR DG++      F  VN++V++ APCSVGILVDRGLG   ++  S V   V +
Sbjct: 576 LPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLGGTSQVVASEVAYKVVI 635

Query: 633 IFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENAARRAGTYRISVAEQEEEMRQ 692
            F GG DDREALAY  ++  HPG+ L+V +F+        A R    R   +E +E+ ++
Sbjct: 636 PFFGGLDDREALAYGMKMVEHPGITLTVYKFV--------AARGTLKRFEKSEHDEKEKK 695

Query: 693 DDECFAYFYERHV----AGGHVAYVEKHMASSAETYSTLKSLEGQYTLVIVGRGGGVNSV 752
           + E    F    +        +AY E+ + S  +  +TLKS+  +  L +VGR   V S+
Sbjct: 696 EKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVVGRNAAVASL 755

Query: 753 LTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHN 776
           +         CPELGP+G +LS SEFS   SVL++  ++
Sbjct: 756 VKS-----TDCPELGPVGRLLSSSEFSTTASVLVVQGYD 773

BLAST of Cp4.1LG12g06710 vs. ExPASy Swiss-Prot
Match: Q9SA37 (Cation/H(+) antiporter 1 OS=Arabidopsis thaliana OX=3702 GN=CHX1 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 6.0e-69
Identity = 199/754 (26.39%), Postives = 357/754 (47.35%), Query Frame = 0

Query: 47  HYLLRPFSQPRTTSDTIVGLVVG--NFMRKELDLSIHK-TLRYIVDFGM---TCYMFVLG 106
           +  L+P  Q    +  + G+V+     +RK  +  + K +  Y + F     T ++F++G
Sbjct: 37  YLFLKPCGQAGPVAQILAGIVLSLLTIIRKVHEFFLQKDSASYYIFFSFLLRTAFVFLIG 96

Query: 107 LEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISF------SKSKEISFILSLSTV 166
           LE+D     K   +++ V   G  S +I     +PF+ F       K   ++F L+    
Sbjct: 97  LEID-LDFMKRNLKNSIVITLG--SLVISGIIWLPFLWFLIRFMQIKGDFLTFYLAFLIT 156

Query: 167 LSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGYLFCQCQETRLSIIN 226
           LS+TA+PV+ R I + K+  S+IGRL I+ G+  +  +     I   F     T    I 
Sbjct: 157 LSNTAAPVVIRSIIDWKLHTSEIGRLAISCGLFIEITNIFIYTIVLSFISGTMTADIFIY 216

Query: 227 GFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGY 286
            F  G ++L    +A        +W+   NP+ K +     +  +  +  +         
Sbjct: 217 SFATGVIILTNRFLA--------SWLPKRNPKEKYLSKAETLAFIILILIIALTIESSNL 276

Query: 287 NPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVESKLSDFEPGQIITW 346
           N TL  F+ G   PREG+  R  I +++Y +     P++F ++G    ++       +  
Sbjct: 277 NSTLFVFIIGLMFPREGKTYRTLIQRLSYPIHEFVLPVYFGYIGFRFSVNSLTKRHYLV- 336

Query: 347 VRLLLLFAIVTVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHIYLAISAKIAGKIS 406
             L +  A+  +GK++G + A + L       + L  +L++KGH  + L  S  +  K  
Sbjct: 337 --LGMTVALSLLGKLLGVLFACSFLKIPKQYWLFLSTMLSVKGHIGLVLLDSNLMYKKWF 396

Query: 407 TSTSIAMVFVIFFTVVHAPKVVAHIIRRARKRTPTH-RMALELLDPSSELKILLCIHGPH 466
           T     M       +     V+  ++ R+++++  H + +LEL D + EL++L C++G  
Sbjct: 397 TPVVHDMFVAALVIMTLLSGVITSLLLRSQEKSFAHIKTSLELFDTTEELRVLTCVYGVR 456

Query: 467 NTGAAINIME----ISRGTANPGVVVYVTDMIELTDEIAATLVQGEGVDTVTVTHTGVTQ 526
           +   +I+++      S GT++     Y+  +I L  +    L+  E  +    ++ G  +
Sbjct: 457 HARGSISLVSALSGFSPGTSSSPFTPYLMHLIPLPKKRKTELLYHELDEDAGNSNGGDDE 516

Query: 527 MRDQVTTAFQRYVDE--NGDGITLRRMLALSAFNCMAQDICILAEDLLGALIILPFHKNQ 586
                       +D       I +R++  ++    M ++IC   EDL  +++ LPFHK+Q
Sbjct: 517 FGTNEGLEINDSIDSFTRDRKIMVRQVKLVAPMENMHEEICNATEDLRVSIVFLPFHKHQ 576

Query: 587 RGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKISRSYVTLNVAVIFIGGKD 646
           R DG  +     FR++NRKV++ A CS+GI VDR +    ++  S    +VA +F GG D
Sbjct: 577 RIDGKTTNDGEVFRHMNRKVLKQAQCSIGIFVDRNITGFHQLHGSDSVQHVAALFFGGPD 636

Query: 647 DREALAYAGRVARHPGVKLSVIRFLV-DADAENAARRAGT------YRISVAEQEEEMRQ 706
           DREAL+    +  +  + L+VI+F+  D+  E     A T      +   V+E + E   
Sbjct: 637 DREALSLCKWLTNNSQIHLTVIQFVADDSKTEKIVGDAVTKENNEVFLEIVSEDQTENET 696

Query: 707 DDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEGQYTLVIVGRGGGVNSVLTYG 766
           D      FY R V  G V ++EK +++  +T + L+ +   Y+L +VG+  G +  +T G
Sbjct: 697 DRIFLEEFYHRFVTTGQVGFIEKRVSNGMQTLTILREIGEMYSLFVVGKNRG-DCPMTSG 756

Query: 767 MNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQH 775
           MNDW++CPELG +GD L+ S      SVL++ +H
Sbjct: 757 MNDWEECPELGTVGDFLASSNMDVNASVLVVQRH 775

BLAST of Cp4.1LG12g06710 vs. NCBI nr
Match: XP_023547506.1 (cation/H(+) antiporter 28-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1546 bits (4003), Expect = 0.0
Identity = 790/790 (100.00%), Postives = 790/790 (100.00%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS
Sbjct: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY
Sbjct: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL
Sbjct: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV
Sbjct: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF
Sbjct: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD
Sbjct: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL
Sbjct: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. NCBI nr
Match: XP_022954237.1 (cation/H(+) antiporter 28-like [Cucurbita moschata] >KAG7014252.1 Cation/H(+) antiporter 28 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1544 bits (3997), Expect = 0.0
Identity = 789/790 (99.87%), Postives = 789/790 (99.87%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS
Sbjct: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY
Sbjct: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL
Sbjct: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV
Sbjct: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPTI GYNPTLSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIIGYNPTLSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF
Sbjct: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD
Sbjct: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL
Sbjct: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. NCBI nr
Match: XP_022991854.1 (cation/H(+) antiporter 28-like [Cucurbita maxima])

HSP 1 Score: 1538 bits (3982), Expect = 0.0
Identity = 783/790 (99.11%), Postives = 788/790 (99.75%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS
Sbjct: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY
Sbjct: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL
Sbjct: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTLAICIGYLFCQCQETRLSI NGFQLGFVLLIQMIMAAKVSP+FMNWV
Sbjct: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSITNGFQLGFVLLIQMIMAAKVSPVFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVI GAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIGGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTS+AMVFVIFFTVVHAPKVVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSLAMVFVIFFTVVHAPKVVAHII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           +RARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 KRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF
Sbjct: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           NCMAQDICILAEDLLGALIILPFHK+QRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD
Sbjct: 541 NCMAQDICILAEDLLGALIILPFHKHQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLI+HQHNLKGEL
Sbjct: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIVHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. NCBI nr
Match: XP_038896873.1 (cation/H(+) antiporter 28 isoform X2 [Benincasa hispida])

HSP 1 Score: 1412 bits (3655), Expect = 0.0
Identity = 709/790 (89.75%), Postives = 755/790 (95.57%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MA TR  EPSRSLA  TCTIRLTEIFG+AT+SILGFFLM+GLCNGVHYLLRPFSQPR TS
Sbjct: 37  MARTRDREPSRSLANATCTIRLTEIFGKATRSILGFFLMVGLCNGVHYLLRPFSQPRITS 96

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLV+GNF+RK+LD+S  KTLRYIVDFGM CYMFVLGLE+DP VLFKAPTRDAKVAY
Sbjct: 97  DTIVGLVIGNFIRKQLDISTIKTLRYIVDFGMICYMFVLGLEVDPYVLFKAPTRDAKVAY 156

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACS IPFISF+KSKEISFI+SLSTVLSSTASPVLTRLIT+LKIG+SDIGRL
Sbjct: 157 AGMISTLILACSIIPFISFAKSKEISFIISLSTVLSSTASPVLTRLITSLKIGKSDIGRL 216

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VI AGMHSDFISTL ICIGYLFC+CQETR+S+I GFQLG +LLIQ ++ AKVSP+FMNWV
Sbjct: 217 VIGAGMHSDFISTLMICIGYLFCKCQETRISLIKGFQLGILLLIQAVLTAKVSPVFMNWV 276

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPT FGYNP LSAFLAGTFLPREGRVSRWAIGK
Sbjct: 277 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTAFGYNPILSAFLAGTFLPREGRVSRWAIGK 336

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTT+F+PIFF WMGVESKLSDFEPGQI+TWVRL+LLFAI TVGKVVGTVIAGAILG
Sbjct: 337 INYLLTTIFFPIFFVWMGVESKLSDFEPGQIMTWVRLILLFAIATVGKVVGTVIAGAILG 396

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAI+AK AGKI+TSTS+ MV VIFFTVVHAPKVVAHII
Sbjct: 397 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSLVMVIVIFFTVVHAPKVVAHII 456

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           +RARKRTPTHRMAL+LLDPSSELKILLC+HGP N+ AAINIMEISRGTANPGVVVYVTDM
Sbjct: 457 QRARKRTPTHRMALQLLDPSSELKILLCVHGPQNSAAAINIMEISRGTANPGVVVYVTDM 516

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIA+TLVQGEGVDTVTVTHTGVTQMRDQVT+A Q YVDENG+GITLRRMLALS F
Sbjct: 517 IELTDEIASTLVQGEGVDTVTVTHTGVTQMRDQVTSAVQSYVDENGEGITLRRMLALSTF 576

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           N MAQDICILAE+L+GALIILPFHK+QRGDGSLSEGQ+ FRYVNRKV+RHAPCSVGILVD
Sbjct: 577 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQSAFRYVNRKVLRHAPCSVGILVD 636

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEK+SRSYV+ NVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 637 RGLGSVEKVSRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 696

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKH+ASS+ETYSTLKSLEG
Sbjct: 697 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 756

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QY L+IVGRGG VNSVLT+GMNDWQQCPELGPIGD+LSGSEFS +TSVLIIHQHNLKGEL
Sbjct: 757 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 816

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 817 DGLDDDFSIM 826

BLAST of Cp4.1LG12g06710 vs. NCBI nr
Match: XP_038896872.1 (cation/H(+) antiporter 28 isoform X1 [Benincasa hispida])

HSP 1 Score: 1412 bits (3655), Expect = 0.0
Identity = 709/790 (89.75%), Postives = 755/790 (95.57%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MA TR  EPSRSLA  TCTIRLTEIFG+AT+SILGFFLM+GLCNGVHYLLRPFSQPR TS
Sbjct: 1   MARTRDREPSRSLANATCTIRLTEIFGKATRSILGFFLMVGLCNGVHYLLRPFSQPRITS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLV+GNF+RK+LD+S  KTLRYIVDFGM CYMFVLGLE+DP VLFKAPTRDAKVAY
Sbjct: 61  DTIVGLVIGNFIRKQLDISTIKTLRYIVDFGMICYMFVLGLEVDPYVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACS IPFISF+KSKEISFI+SLSTVLSSTASPVLTRLIT+LKIG+SDIGRL
Sbjct: 121 AGMISTLILACSIIPFISFAKSKEISFIISLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VI AGMHSDFISTL ICIGYLFC+CQETR+S+I GFQLG +LLIQ ++ AKVSP+FMNWV
Sbjct: 181 VIGAGMHSDFISTLMICIGYLFCKCQETRISLIKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPT FGYNP LSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTAFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTT+F+PIFF WMGVESKLSDFEPGQI+TWVRL+LLFAI TVGKVVGTVIAGAILG
Sbjct: 301 INYLLTTIFFPIFFVWMGVESKLSDFEPGQIMTWVRLILLFAIATVGKVVGTVIAGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAI+AK AGKI+TSTS+ MV VIFFTVVHAPKVVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSLVMVIVIFFTVVHAPKVVAHII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           +RARKRTPTHRMAL+LLDPSSELKILLC+HGP N+ AAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCVHGPQNSAAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIA+TLVQGEGVDTVTVTHTGVTQMRDQVT+A Q YVDENG+GITLRRMLALS F
Sbjct: 481 IELTDEIASTLVQGEGVDTVTVTHTGVTQMRDQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           N MAQDICILAE+L+GALIILPFHK+QRGDGSLSEGQ+ FRYVNRKV+RHAPCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQSAFRYVNRKVLRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEK+SRSYV+ NVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKVSRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKH+ASS+ETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QY L+IVGRGG VNSVLT+GMNDWQQCPELGPIGD+LSGSEFS +TSVLIIHQHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. ExPASy TrEMBL
Match: A0A6J1GQH7 (cation/H(+) antiporter 28-like OS=Cucurbita moschata OX=3662 GN=LOC111456554 PE=4 SV=1)

HSP 1 Score: 1544 bits (3997), Expect = 0.0
Identity = 789/790 (99.87%), Postives = 789/790 (99.87%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS
Sbjct: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY
Sbjct: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL
Sbjct: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV
Sbjct: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPTI GYNPTLSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIIGYNPTLSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF
Sbjct: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD
Sbjct: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL
Sbjct: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. ExPASy TrEMBL
Match: A0A6J1JVZ7 (cation/H(+) antiporter 28-like OS=Cucurbita maxima OX=3661 GN=LOC111488376 PE=4 SV=1)

HSP 1 Score: 1538 bits (3982), Expect = 0.0
Identity = 783/790 (99.11%), Postives = 788/790 (99.75%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS
Sbjct: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY
Sbjct: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL
Sbjct: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTLAICIGYLFCQCQETRLSI NGFQLGFVLLIQMIMAAKVSP+FMNWV
Sbjct: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSITNGFQLGFVLLIQMIMAAKVSPVFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVI GAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIGGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTS+AMVFVIFFTVVHAPKVVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSLAMVFVIFFTVVHAPKVVAHII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           +RARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 KRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF
Sbjct: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           NCMAQDICILAEDLLGALIILPFHK+QRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD
Sbjct: 541 NCMAQDICILAEDLLGALIILPFHKHQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLI+HQHNLKGEL
Sbjct: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIVHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. ExPASy TrEMBL
Match: A0A0A0KBF5 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G426570 PE=4 SV=1)

HSP 1 Score: 1412 bits (3654), Expect = 0.0
Identity = 709/790 (89.75%), Postives = 754/790 (95.44%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MA TR HEPSRSLA +TCTIRLTEIFG+AT+SILGFF+MMGLCNGVHYLLRPFSQPR TS
Sbjct: 1   MAKTRNHEPSRSLANDTCTIRLTEIFGKATRSILGFFIMMGLCNGVHYLLRPFSQPRITS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLVVGNF+RK+LD+S  KTLRYIVDFGM CYMFVLGLE+DP V+FKAPTRDAKVAY
Sbjct: 61  DTIVGLVVGNFIRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVIFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACS IPFI   KSKEISFILSLSTVLSSTASPVLTRLIT+LKIG+SDIGRL
Sbjct: 121 AGMISTLILACSIIPFIGMIKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTL IC+GYLFC+CQETR+S I GFQLG +LLIQ ++ AKVSP+FMNWV
Sbjct: 181 VIAAGMHSDFISTLMICVGYLFCKCQETRISFIKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPT+FGYNP LSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKL+DFEPGQI+TWVRL+LLFAI T+GKVVGTVIAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLILLFAIATLGKVVGTVIAGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAI+AK AGKI+TSTSI MV VIFFT+VHAPKVVAHII
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSIVMVIVIFFTIVHAPKVVAHII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           +RARKRTPTHRMAL+LLDPSSELKILLCIHGP NT AAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIA+TLVQGEGVD+VTVTHTGVTQMR+QVT+A Q YVDENG+GITLRRMLALS F
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           N MAQDICILAE+L+GALIILPFHK+QRGDGSLSEGQ  FRYVNRKV+RHAPCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQTAFRYVNRKVLRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYV+ NVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADA NA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAVNA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKH+ASS+ETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QY L+IVGRGG VN+VLT+GMNDWQQCPELGPIGD+LSGSEFS +TSVLIIHQHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNTVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. ExPASy TrEMBL
Match: A0A1S3CE27 (cation/H(+) antiporter 28 OS=Cucumis melo OX=3656 GN=LOC103499939 PE=4 SV=1)

HSP 1 Score: 1410 bits (3650), Expect = 0.0
Identity = 709/790 (89.75%), Postives = 758/790 (95.95%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MA TRVH+PSRSLA ++CTIRLTEIFGRAT+SILGFFLMM LCNGVHYLLRPFSQPR TS
Sbjct: 1   MAKTRVHQPSRSLAIDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRITS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGLVVGNF+RK+LD+S  KTLRYIVDFGM CYMFVLGLE+DP VLFKAPTRDAKVAY
Sbjct: 61  DTIVGLVVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AG+ISTLILACS IPFIS++KSKEISFILSLSTVLSSTASPVLTRLIT+LKIG+SDIGRL
Sbjct: 121 AGLISTLILACSIIPFISYAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTL IC+GYLFC+CQETR+S++ GFQLG +LLIQ ++ AKVSP+FMNWV
Sbjct: 181 VIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPT+FGYNP LSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKL+DFEPGQI+TWVRL+LLFAI T+GKVVGTVIAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVRLMLLFAIATIGKVVGTVIAGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAI+AK AGKI+TSTS+ MV VIFFTVVHAPKVV  II
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVECII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           +RARKRTPTHRMAL+LLDPSSELKILLCIHGP NT AAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIA+TLVQGEGVD+VTVTHTGVTQMR+QVT+A Q YVDENG+GITLRRMLALS F
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           N MAQDICILAE+L+GALIILPFHK+QRGDGSLSEGQA FRYVNRKV+RHAPCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQAAFRYVNRKVLRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYV+ NVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQE+EMRQDDECFAYFYERHVAGGHVAYVEKH+ASS+ETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QY L+IVGRGG VNSVLT+GMNDWQQCPELGPIGD+LSGSEFS +TSVLIIHQHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. ExPASy TrEMBL
Match: A0A5D3CFY8 (Cation/H(+) antiporter 28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00710 PE=4 SV=1)

HSP 1 Score: 1410 bits (3649), Expect = 0.0
Identity = 709/790 (89.75%), Postives = 756/790 (95.70%), Query Frame = 0

Query: 1   MAMTRVHEPSRSLAGNTCTIRLTEIFGRATKSILGFFLMMGLCNGVHYLLRPFSQPRTTS 60
           MA TRVH+PSRSLA ++CTIRLTEIFGRAT+SILGFFLMM LCNGVHYLLRPFSQPR TS
Sbjct: 1   MAKTRVHQPSRSLAIDSCTIRLTEIFGRATRSILGFFLMMVLCNGVHYLLRPFSQPRITS 60

Query: 61  DTIVGLVVGNFMRKELDLSIHKTLRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAY 120
           DTIVGL VGNF+RK+LD+S  KTLRYIVDFGM CYMFVLGLE+DP VLFKAPTRDAKVAY
Sbjct: 61  DTIVGLAVGNFVRKQLDISTIKTLRYIVDFGMVCYMFVLGLEVDPYVLFKAPTRDAKVAY 120

Query: 121 AGMISTLILACSTIPFISFSKSKEISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRL 180
           AGMISTLILACS IPFISF+KSKEISFILSLSTVLSSTASPVLTRLIT+LKIG+SDIGRL
Sbjct: 121 AGMISTLILACSIIPFISFAKSKEISFILSLSTVLSSTASPVLTRLITSLKIGKSDIGRL 180

Query: 181 VIAAGMHSDFISTLAICIGYLFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWV 240
           VIAAGMHSDFISTL IC+GYLFC+CQETR+S++ GFQLG +LLIQ ++ AKVSP+FMNWV
Sbjct: 181 VIAAGMHSDFISTLLICVGYLFCKCQETRISVMKGFQLGILLLIQAVLTAKVSPVFMNWV 240

Query: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGK 300
           NNENPEGKPMKGPHLVLAVAFMAFLCCCPT+FGYNP LSAFLAGTFLPREGRVSRWAIGK
Sbjct: 241 NNENPEGKPMKGPHLVLAVAFMAFLCCCPTVFGYNPILSAFLAGTFLPREGRVSRWAIGK 300

Query: 301 INYLLTTVFYPIFFFWMGVESKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILG 360
           INYLLTTVFYPIFFFWMGVESKL+DFEPGQI+TWV L+LLFAI T+GKVVGTVIAGAILG
Sbjct: 301 INYLLTTVFYPIFFFWMGVESKLTDFEPGQIMTWVTLILLFAIATIGKVVGTVIAGAILG 360

Query: 361 FHWPESVALGLLLTMKGHFHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHII 420
           FHWPESVALGLLLTMKGHFHIYLAI+AK AGKI+TSTS+ MV VIFFTVVHAPKVV  II
Sbjct: 361 FHWPESVALGLLLTMKGHFHIYLAIAAKTAGKITTSTSMVMVIVIFFTVVHAPKVVERII 420

Query: 421 RRARKRTPTHRMALELLDPSSELKILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDM 480
           +RARKRTPTHRMAL+LLDPSSELKILLCIHGP NT AAINIMEISRGTANPGVVVYVTDM
Sbjct: 421 QRARKRTPTHRMALQLLDPSSELKILLCIHGPQNTPAAINIMEISRGTANPGVVVYVTDM 480

Query: 481 IELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAF 540
           IELTDEIA+TLVQGEGVD+VTVTHTGVTQMR+QVT+A Q YVDENG+GITLRRMLALS F
Sbjct: 481 IELTDEIASTLVQGEGVDSVTVTHTGVTQMREQVTSAVQSYVDENGEGITLRRMLALSTF 540

Query: 541 NCMAQDICILAEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVD 600
           N MAQDICILAE+L+GALIILPFHK+QRGDGSLSEGQA FRYVNRKV+RHAPCSVGILVD
Sbjct: 541 NSMAQDICILAEELMGALIILPFHKSQRGDGSLSEGQAAFRYVNRKVLRHAPCSVGILVD 600

Query: 601 RGLGSVEKISRSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660
           RGLGSVEKISRSYV+ NVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA
Sbjct: 601 RGLGSVEKISRSYVSQNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENA 660

Query: 661 ARRAGTYRISVAEQEEEMRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEG 720
           ARRAGTYRISVAEQE+EMRQDDECFAYFYERHVAGGHVAYVEKH+ASS+ETYSTLKSLEG
Sbjct: 661 ARRAGTYRISVAEQEDEMRQDDECFAYFYERHVAGGHVAYVEKHLASSSETYSTLKSLEG 720

Query: 721 QYTLVIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGEL 780
           QY L+IVGRGG VNSVLT+GMNDWQQCPELGPIGD+LSGSEFS +TSVLIIHQHNLKGEL
Sbjct: 721 QYALIIVGRGGKVNSVLTFGMNDWQQCPELGPIGDILSGSEFSVRTSVLIIHQHNLKGEL 780

Query: 781 DGLDDDFSIM 790
           DGLDDDFSIM
Sbjct: 781 DGLDDDFSIM 790

BLAST of Cp4.1LG12g06710 vs. TAIR 10
Match: AT3G52080.1 (cation/hydrogen exchanger 28 )

HSP 1 Score: 808.1 bits (2086), Expect = 6.1e-234
Identity = 415/775 (53.55%), Postives = 554/775 (71.48%), Query Frame = 0

Query: 33  ILGFFLMMGLCNGVHYLLRPFSQPRTTSDTIVGLVVGNFMRKELDLS--IHKTLRYIVDF 92
           IL F  +  +   +HYL++P  QP  T+D  +GL++GN  R     S     TL  I++F
Sbjct: 27  ILVFIAIFVVRTLLHYLMKPLGQPYLTTDFAIGLILGNIPRFRGAFSGPYSITLNNIIEF 86

Query: 93  GMTCYMFVLGLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSKEISFILS 152
           GM C+MFV+GLEM+P VL + PT+DA +AY  MI+T +LA  T PF+ ++K+    F L+
Sbjct: 87  GMICHMFVMGLEMNPSVLLRPPTKDAFIAYTSMITTFVLAFVTTPFLHYTKTSPYIFSLA 146

Query: 153 LSTVLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGYLFCQCQETRL 212
           LS + SST SP+LTR+I NLKI +SD+G+L  AAG+H+D ISTL  C G++F   ++   
Sbjct: 147 LSLMASSTGSPILTRVIANLKIRKSDLGKLASAAGVHTDMISTLLYCFGFIFFPTEKPLA 206

Query: 213 SIINGFQLGFVL----LIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVAFMAFLC 272
             ++ F    ++    L Q+   + VSPIF+NWVNNENPEGKP+KG HLV+++AF+  +C
Sbjct: 207 RPLHRFFRALLMFCLFLAQVTFTSIVSPIFLNWVNNENPEGKPLKGSHLVMSLAFVVLIC 266

Query: 273 CCPT---IFGYNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVESKL 332
             PT      YNP LSAF AG FLP +GR+S+W I KINYLL+TVFYPIFFFW+G    +
Sbjct: 267 SFPTWPPESMYNPILSAFTAGLFLPNKGRMSKWIINKINYLLSTVFYPIFFFWVGFIIHM 326

Query: 333 SDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHIYL 392
            +F+    + WVR   L   V  GKV GTV+ G +LG+H PE+ +LGLLLT KGHFH+YL
Sbjct: 327 RNFDITDKMAWVRFFSLLGTVIAGKVTGTVLCGLLLGYHVPETASLGLLLTTKGHFHVYL 386

Query: 393 AISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHIIRRARKRTPTHRMALELLDPSSEL 452
           A  A    ++ ++T   ++F+I FTVV++P VV  II+RARKR P H MAL+ LDP++EL
Sbjct: 387 AALAIRTNRVKSTTGALIIFIIVFTVVYSPFVVMDIIKRARKRVPVHIMALQWLDPTTEL 446

Query: 453 KILLCIHGPHNTGAAINIMEISRGTANPGVVVYVTDMIELTDEIAATLVQGEGV----DT 512
           +IL+ +HGPHN G+ +N+MEI  G   PG + Y TDM+ELTDEIAATL +G G     D+
Sbjct: 447 RILIGLHGPHNIGSTLNVMEICHGGREPGSIFYATDMVELTDEIAATLKKGGGAGQSNDS 506

Query: 513 VTVTHTGVTQMRDQVTTAFQRYVD-ENGDGITLRRMLALSAFNCMAQDICILAEDLLGAL 572
           VTVT   VT+MR+ +T A   Y +  NG G+T+RRMLALS F  MA D+C LA++L+ ++
Sbjct: 507 VTVTDRSVTEMRESITAAVNGYGELRNGQGVTVRRMLALSTFVTMAHDVCGLADELMVSI 566

Query: 573 IILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKISRSYVTLNV 632
           IILPFHK    DG+L  G AGFR+VNRK++++APCSVGILVDR  G  E+  R   ++ +
Sbjct: 567 IILPFHKRLNPDGTLDAGHAGFRHVNRKILKNAPCSVGILVDRSFGQTEEAWRPGASMGI 626

Query: 633 AVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENAARRAGTY-RISVAEQEEE 692
           A+IFIGG+DDREALA+A +VARHP VKL VIRFL D  ++NA +R+    R SV +QEEE
Sbjct: 627 AIIFIGGRDDREALAFAAQVARHPAVKLKVIRFLEDKSSQNAQKRSSILNRASVVDQEEE 686

Query: 693 MRQDDECFAYFYERHVA-GGHVAYVEKHMASSAETYSTLKSLEGQYTLVIVGRGGG-VNS 752
           M+ DDECFA FYER++A GG V+Y+EKH+ +S+ET++ LKSL+G+Y LVIVGRGGG  +S
Sbjct: 687 MKLDDECFAEFYERYIAGGGRVSYMEKHLTNSSETFTALKSLDGEYGLVIVGRGGGRASS 746

Query: 753 VLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKGELDGLDDDFSIM 791
            LT G+NDWQQCPELGPIGDVLSGS+FS  TS+LII Q   +G+L+GL DDF+I+
Sbjct: 747 GLTTGLNDWQQCPELGPIGDVLSGSDFSHNTSMLIIQQQRTRGQLEGLHDDFTIL 801

BLAST of Cp4.1LG12g06710 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 364.4 bits (934), Expect = 2.3e-100
Identity = 222/745 (29.80%), Postives = 384/745 (51.54%), Query Frame = 0

Query: 48  YLLRPFSQPRTTSDTIVGLVVGNFMRKELDLSIH--------KTLRYIVDFGMTCYMFVL 107
           ++L+PF QPR  S+ + G+V+G  +        H          L  + + G+  ++F++
Sbjct: 56  FILKPFRQPRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLV 115

Query: 108 GLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSK------EISFILSLST 167
           G+EMD  V+ K   R   +A  GM+   ++  +     SFS  +      + ++IL L  
Sbjct: 116 GVEMDIMVVRKTGKRALTIAIGGMVLPFLIGAA----FSFSMHRSEDHLGQGTYILFLGV 175

Query: 168 VLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGYLFCQCQETRLSII 227
            LS TA PVL R++  LK+  ++IGR+ ++A + +D  + + + +     +  +T  + +
Sbjct: 176 ALSVTAFPVLARILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASL 235

Query: 228 NGFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVAFMAFLCCCPTIFG 287
                  V +   +   +     + W+  + PEG+     H+ L +  +          G
Sbjct: 236 WVMISSAVFIAVCVFVVRPG---IAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIG 295

Query: 288 YNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVESKLSDFEPGQIIT 347
            +    AF+ G  +P  G +    I K+   ++ +  P+FF   G+++ ++  +     T
Sbjct: 296 THSVFGAFVFGLVIP-NGPLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGP--AT 355

Query: 348 WVRLLLLFAIVTVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHIYLAISAKIAGKI 407
           W+ L L+  +   GKV+GTVI     G    E + LGLLL  KG   + +    K    +
Sbjct: 356 WLTLFLVIFLACAGKVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVL 415

Query: 408 STSTSIAMVFVIFFTVVHAPKVVAHIIRRARKRTPTHRMALELLDPSSELKILLCIHGPH 467
              T   MV V          +V  + +  +K     R  ++   P SEL++L+C+H P 
Sbjct: 416 DDETFATMVLVALVMTGVITPIVTILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPR 475

Query: 468 NTGAAINIMEISRGTANPGVVVYVTDMIELTDEIAATLVQGEGVDTVTVTHTGVTQMRDQ 527
           N    IN++E S  T    + +YV  ++ELT   +A L+      +            D 
Sbjct: 476 NVPTIINLLEASHPTKRSPICIYVLHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDH 535

Query: 528 VTTAFQRYVDENGDGITLRRMLALSAFNCMAQDICILAEDLLGALIILPFHKNQRGDGSL 587
           +  AF+ Y +++   + ++ + A+S ++ M +D+C LAED   + II+PFHK Q  DG +
Sbjct: 536 IINAFENY-EQHAAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGM 595

Query: 588 SEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKISRSYVTLNVAVIFIGGKDDREALA 647
                 +R VN+ ++ ++PCSVGILVDRGL    +++ + V+L VAV+F GG DDREALA
Sbjct: 596 ESTNPAYRLVNQNLLENSPCSVGILVDRGLNGATRLNSNTVSLQVAVLFFGGPDDREALA 655

Query: 648 YAGRVARHPGVKLSVIRFLVDAD-AENAARRA---GTYRISVAEQEEEMRQDDECFAYFY 707
           YA R+A+HPG+ L+V+RF+ D D A+ A+ RA      +I   +  ++ + DD+    F 
Sbjct: 656 YAWRMAQHPGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFR 715

Query: 708 ERHVAGGHVAYVEKHMASSAETYSTLKSLEGQYTLVIVGRGGGVNSVLTYGMNDWQQCPE 767
             +     + Y+EK +++  ET + ++S++  + L IVGRG G++S LT G+ DW +CPE
Sbjct: 716 AENAEYESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPE 775

Query: 768 LGPIGDVLSGSEFSAKTSVLIIHQH 775
           LG IGD+L+ S+F+A  SVL++ Q+
Sbjct: 776 LGAIGDLLASSDFAATVSVLVVQQY 789

BLAST of Cp4.1LG12g06710 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 317.8 bits (813), Expect = 2.5e-86
Identity = 232/774 (29.97%), Postives = 387/774 (50.00%), Query Frame = 0

Query: 32  SILGFFLMMGLCNGVHYLLRPFSQPRTTSDTIVGLVVGNFM----RKELDLSIHK----T 91
           +IL   +++ L   + YLLRP  QPR  ++ I G+++G  +    +  LD    K     
Sbjct: 34  AILQIVIVIVLTRVLAYLLRPLRQPRVIAEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTV 93

Query: 92  LRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSK 151
           L  + + G+  ++F+ GLE+D   L +   +   +A AG+     L   +   +  + SK
Sbjct: 94  LETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKATISK 153

Query: 152 EI---SFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGY 211
            +   +F++ +   LS TA PVL R++  LK+  ++IGRL ++A   +D  + + + +  
Sbjct: 154 GVNSTAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILLALAI 213

Query: 212 LFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVA 271
                  + L  +  F  G   +I    A+ + P    W++    EG+P++  ++   +A
Sbjct: 214 ALSGSNTSPLVSLWVFLSGCAFVIG---ASFIIPPIFRWISRRCHEGEPIEETYICATLA 273

Query: 272 FMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVE 331
            +          G +    AF+ G  +P+EG  +   + K+  L++ +F P++F   G++
Sbjct: 274 VVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPLYFVASGLK 333

Query: 332 SKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILGFHWP--ESVALGLLLTMKGH 391
           + ++  +  Q  +W  L+L+ A    GK++GT+  G  L F  P  E++ LG L+  KG 
Sbjct: 334 TNVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLGFLMNTKGL 393

Query: 392 FHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHI---IRRARKRTPTHRMALE 451
             + +    K    ++  T   MV +  FT      VV  +    RRA+K       A+E
Sbjct: 394 VELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVE 453

Query: 452 LLDPSSELKILLCIHGPHNTGAAINIMEISRG-TANPGVVVYVTDMIELTDEIAATL--- 511
             + +++L+IL C HG  +  + IN++E SRG     G+ VY   + EL++  +A L   
Sbjct: 454 RENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVH 513

Query: 512 -VQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAFNCMAQDICIL 571
            V+  G+        GV    DQV  AFQ +  +    + +R M A+S+ + + +DIC  
Sbjct: 514 KVRKNGMP--FWNRRGVNADADQVVVAFQAF--QQLSRVNVRPMTAISSMSDIHEDICTT 573

Query: 572 AEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKIS 631
           A     A++ILPFHK+Q+ DGSL   +  +R+VNR+V+  APCSVGI VDRGLG   ++S
Sbjct: 574 AVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRGLGGSSQVS 633

Query: 632 RSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENAARRAGTYRIS 691
              V+ +V V+F GG DDREALAY  R+A HPG+ L+V RF+V  +     R      + 
Sbjct: 634 AQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPE-----RVGEIVNVE 693

Query: 692 VAEQEEE------MRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEGQYTL 751
           V+    E      ++ D+E  +   +       V +VEK + ++A    +      +  L
Sbjct: 694 VSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVRRSNL 753

Query: 752 VIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKG 779
            +VGR  G    L    N   +CPELGP+G +L   E S K SVL+I Q+N  G
Sbjct: 754 FLVGRMPGGEIALAIREN--SECPELGPVGSLLISPESSTKASVLVIQQYNGTG 789

BLAST of Cp4.1LG12g06710 vs. TAIR 10
Match: AT3G17630.1 (cation/H+ exchanger 19 )

HSP 1 Score: 308.1 bits (788), Expect = 2.0e-83
Identity = 221/759 (29.12%), Postives = 374/759 (49.28%), Query Frame = 0

Query: 33  ILGFFLMMGLCNGVHYLLRPFSQPRTTSDTIVGLVVG----NFMRKELDLSIHK----TL 92
           IL   L++     + Y L+P  QPR  ++ I G+++G       +  LD    K     L
Sbjct: 36  ILQIVLVVVFTRLLAYFLKPLKQPRVIAEIIGGILLGPSALGRSKAYLDTIFPKKSLTVL 95

Query: 93  RYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSK- 152
             + + G+  ++F++GLE+D   + K   +   +A AG+    I+   T   +S + SK 
Sbjct: 96  DTLANIGLLFFLFLVGLELDFAAIKKTGKKSLLIAIAGISLPFIVGVGTSFVLSATISKG 155

Query: 153 --EISFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGYL 212
             ++ FI+ +   LS TA PVL R++  LK+  +DIGR+ ++A   +D  + + + +   
Sbjct: 156 VDQLPFIVFMGVALSITAFPVLARILAELKLLTTDIGRMAMSAAGVNDVAAWILLALAIA 215

Query: 213 FCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVAF 272
                 + L  +     G   +I  ++A K     + ++    PEG+P+K  ++ + +  
Sbjct: 216 LSGDGTSPLVSVWVLLCGTGFVIFAVVAIKP---LLAYMARRCPEGEPVKELYVCVTLTV 275

Query: 273 MAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVES 332
           +          G +    AF+ G   P+EG   R    KI  L++ +  P++F   G+++
Sbjct: 276 VLAASFVTDTIGIHALFGAFVVGIVAPKEGPFCRILTEKIEDLVSGLLLPLYFAASGLKT 335

Query: 333 KLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILGFHWPESVALGLLLTMKGHFHI 392
            ++     Q  +W  L+L+      GK+VGTV +  +    + E+V LG L+  KG   +
Sbjct: 336 DVTTIRGAQ--SWGLLVLVILTTCFGKIVGTVGSSMLCKVPFREAVTLGFLMNTKGLVEL 395

Query: 393 YLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHIIRRARKRTPTHRMALELLDPSS 452
            +    K    ++      +V +  FT      +V  I + ARK  P     ++  D  S
Sbjct: 396 IVLNIGKDRKVLNDQAFAILVLMALFTTFITTPIVMLIYKPARKGAPYKHRTIQRKDHDS 455

Query: 453 ELKILLCIHGPHNTGAAINIMEISRGTANPG-VVVYVTDMIELTDEIAATLVQGEGVDTV 512
           EL+IL C H   N    IN++E SRGT   G + VY   ++EL++  +A  +  +  +  
Sbjct: 456 ELRILACFHSTRNIPTLINLIESSRGTGKKGRLCVYAMHLMELSERSSAIAMVHKARNNG 515

Query: 513 TVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAFNCMAQDICILAEDLLGALII 572
                 + +  DQ+  AF+ Y  ++   + +R M A+S  + + +DIC  A     A+I+
Sbjct: 516 LPIWNKIERSTDQMVIAFEAY--QHLRAVAVRPMTAISGLSSIHEDICTSAHQKRVAMIL 575

Query: 573 LPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKISRSYVTLNVAV 632
           LPFHK+QR DG++      F  VN++V++ APCSVGILVDRGLG   ++  S V   V +
Sbjct: 576 LPFHKHQRMDGAMESIGHRFHEVNQRVLQRAPCSVGILVDRGLGGTSQVVASEVAYKVVI 635

Query: 633 IFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENAARRAGTYRISVAEQEEEMRQ 692
            F GG DDREALAY  ++  HPG+ L+V +F+        A R    R   +E +E+ ++
Sbjct: 636 PFFGGLDDREALAYGMKMVEHPGITLTVYKFV--------AARGTLKRFEKSEHDEKEKK 695

Query: 693 DDECFAYFYERHV----AGGHVAYVEKHMASSAETYSTLKSLEGQYTLVIVGRGGGVNSV 752
           + E    F    +        +AY E+ + S  +  +TLKS+  +  L +VGR   V S+
Sbjct: 696 EKETDEEFVRELMNDPRGNESLAYEERVVESKDDIIATLKSM-SKCNLFVVGRNAAVASL 755

Query: 753 LTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHN 776
           +         CPELGP+G +LS SEFS   SVL++  ++
Sbjct: 756 VKS-----TDCPELGPVGRLLSSSEFSTTASVLVVQGYD 773

BLAST of Cp4.1LG12g06710 vs. TAIR 10
Match: AT5G41610.2 (cation/H+ exchanger 18 )

HSP 1 Score: 298.9 bits (764), Expect = 1.2e-80
Identity = 215/714 (30.11%), Postives = 358/714 (50.14%), Query Frame = 0

Query: 84  LRYIVDFGMTCYMFVLGLEMDPCVLFKAPTRDAKVAYAGMISTLILACSTIPFISFSKSK 143
           L  + + G+  ++F+ GLE+D   L +   +   +A AG+     L   +   +  + SK
Sbjct: 26  LETLANLGLLFFLFLAGLEIDTKALRRTGKKALGIALAGITLPFALGIGSSFVLKATISK 85

Query: 144 EI---SFILSLSTVLSSTASPVLTRLITNLKIGRSDIGRLVIAAGMHSDFISTLAICIGY 203
            +   +F++ +   LS TA PVL R++  LK+  ++IGRL ++A   +D  + + + +  
Sbjct: 86  GVNSTAFLVFMGVALSITAFPVLARILAELKLLTTEIGRLAMSAAAVNDVAAWILLALAI 145

Query: 204 LFCQCQETRLSIINGFQLGFVLLIQMIMAAKVSPIFMNWVNNENPEGKPMKGPHLVLAVA 263
                  + L  +  F  G   +I    A+ + P    W++    EG+P++  ++   +A
Sbjct: 146 ALSGSNTSPLVSLWVFLSGCAFVIG---ASFIIPPIFRWISRRCHEGEPIEETYICATLA 205

Query: 264 FMAFLCCCPTIFGYNPTLSAFLAGTFLPREGRVSRWAIGKINYLLTTVFYPIFFFWMGVE 323
            +          G +    AF+ G  +P+EG  +   + K+  L++ +F P++F   G++
Sbjct: 206 VVLVCGFITDAIGIHSMFGAFVVGVLIPKEGPFAGALVEKVEDLVSGLFLPLYFVASGLK 265

Query: 324 SKLSDFEPGQIITWVRLLLLFAIVTVGKVVGTVIAGAILGFHWP--ESVALGLLLTMKGH 383
           + ++  +  Q  +W  L+L+ A    GK++GT+  G  L F  P  E++ LG L+  KG 
Sbjct: 266 TNVATIQGAQ--SWGLLVLVTATACFGKILGTL--GVSLAFKIPMREAITLGFLMNTKGL 325

Query: 384 FHIYLAISAKIAGKISTSTSIAMVFVIFFTVVHAPKVVAHI---IRRARKRTPTHRMALE 443
             + +    K    ++  T   MV +  FT      VV  +    RRA+K       A+E
Sbjct: 326 VELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITTPVVMAVYKPARRAKKEGEYKHRAVE 385

Query: 444 LLDPSSELKILLCIHGPHNTGAAINIMEISRG-TANPGVVVYVTDMIELTDEIAATL--- 503
             + +++L+IL C HG  +  + IN++E SRG     G+ VY   + EL++  +A L   
Sbjct: 386 RENTNTQLRILTCFHGAGSIPSMINLLEASRGIEKGEGLCVYALHLRELSERSSAILMVH 445

Query: 504 -VQGEGVDTVTVTHTGVTQMRDQVTTAFQRYVDENGDGITLRRMLALSAFNCMAQDICIL 563
            V+  G+        GV    DQV  AFQ +  +    + +R M A+S+ + + +DIC  
Sbjct: 446 KVRKNGMP--FWNRRGVNADADQVVVAFQAF--QQLSRVNVRPMTAISSMSDIHEDICTT 505

Query: 564 AEDLLGALIILPFHKNQRGDGSLSEGQAGFRYVNRKVIRHAPCSVGILVDRGLGSVEKIS 623
           A     A++ILPFHK+Q+ DGSL   +  +R+VNR+V+  APCSVGI VDRGLG   ++S
Sbjct: 506 AVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRVLLQAPCSVGIFVDRGLGGSSQVS 565

Query: 624 RSYVTLNVAVIFIGGKDDREALAYAGRVARHPGVKLSVIRFLVDADAENAARRAGTYRIS 683
              V+ +V V+F GG DDREALAY  R+A HPG+ L+V RF+V  +     R      + 
Sbjct: 566 AQDVSYSVVVLFFGGPDDREALAYGLRMAEHPGIVLTVFRFVVSPE-----RVGEIVNVE 625

Query: 684 VAEQEEE------MRQDDECFAYFYERHVAGGHVAYVEKHMASSAETYSTLKSLEGQYTL 743
           V+    E      ++ D+E  +   +       V +VEK + ++A    +      +  L
Sbjct: 626 VSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQIENAAVDVRSAIEEVRRSNL 685

Query: 744 VIVGRGGGVNSVLTYGMNDWQQCPELGPIGDVLSGSEFSAKTSVLIIHQHNLKG 779
            +VGR  G    L    N   +CPELGP+G +L   E S K SVL+I Q+N  G
Sbjct: 686 FLVGRMPGGEIALAIREN--SECPELGPVGSLLISPESSTKASVLVIQQYNGTG 721