Cp4.1LG12g06450 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g06450
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectin lyase-like superfamily protein
Location	Cp4.1LG12: 7615634 .. 7617878 (+)
RNA-Seq Expression	Cp4.1LG12g06450
Synteny	Cp4.1LG12g06450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATTATTGAAACTAGATTTGGGACCAAAGTGAGGCATGGTTATGGGAAGAACATGGGAGGCTTATCTCCAATCCCACTGCCCTTTTCTTCTTCCTTTTTCTCAAACACTCAATGCCACTCTCCATTTTGTGTATTAAAGGTCAATGCCTACATGAATTTGGTCGGTTCCACTGCTCAAATAATCAATCCAAAAGTAATAATTTCAACATCAACCCTTTGATTCACCTTCCACTTTCACTTAAAGTTGTGTTTTTTCTCTCATTATATAGCCAAAGATTCAGCCTTTCTTGCTTTGTGTTCATCCCATGTTGAAATACCCTCTTGGGTTCTTGAGAAATCCAAGTAATTTCGTTGAAGAAAGTGTTTTTTGAGTGTTTTGAGACATACCCATCTCAAAAAATGTCTAATTTTGTGTTGTCATTGTCATTTTGTGTTACTTTTTTGTGTTGTCTTCTTTGTTCTTCTCAAGCTCGATGGCATCATCATTCCAAGCAAAAACACTCTCACCATCATAAACGTAGCTACATTTCACATCCTCCTACTCCTTCTCCTTCACCGGGCGATGGCGATTACATTAACGAAACGAATATCTTCGACGTACGAGGCTTTGGTGCTGTCGGCGATGGCGTTGCTGACGACACCGAGGCGTTTAAGATGGCTTGGGATACGGCGTGCCAGAGTGACAATGCTGCCGTCGTGCTCGTTCCTTATGGCTACTCGTTTATGATTCAATCAACAATCTTTACTGGCCCTTGCCATGGTCATCTCATGTTGTGGGTTGATGGCACGTTGATGCCACCGGATGGCCCTGATGCCTGGCCGAGGAATGCTAGTCGCCGGCAGTGGTTGGTGTTCTATAGAATTGATGGGATGTCCTTAGAGGGTGGAGGTGTGGTTGATGGGCGAGGTCAAAAATGGTGGGATCTTCCTTGCAAACCACACAAGGTATTGGTTCGATCTTCTTCGATGTTTAAGTAAATGTCTCCGACGTTCTAACACGAAGATATTTTTAAACTTTAGGGACCAAATGGAACCACGTTACCAGGACCGTGTGACAGCCCAATTGTAAGTTCGTGCTGACCATATGATACTTCTCTATTAAGATCGGGATATGATGAAATTACTTATGTGTATCACATTGTAATGATTTTAGGCTATAAGGTTCTTCATGAGCTCGAATTTGACTGTCCAAGGACTTAAATTCACAAACAGTCCTCAGTTTCACTTCAGATTCGACGGTTGCAAGAATGTTCATATAGAGTCGATTCATATAAAGTCTCCGGGTCTTAGTCCCAACACTGATGGTATCCACATCGAGAACACAAATGGCGTCGGGATATATAATTCAGTCATTTACAATGGTAAGTTGGTTGAAAAGTATCTTTGCTAAGAGTATGAATTATAAATCCTGATTCTACTCATCGTTATGTAGGGGATGATTGTGTATCAATTGGGTCGGGTTCATATGATGTCGATATCCGGAACATCACTTGTGGACCAAGTCATGGAATTAGGTATGGTATAAATTAGTAGTAGTTCAATTAGTTAAGATATTAATAATCTCCTGTTCTTAGACCTTTTTATTTCGGCCCAGTATCGGAAGTCTTGGTGTTCGAAACTCGAGAGCTTGTGTTACAAACATAACGGTCAGGGACTCGGTGATCAAAGACTCAGACAACGGTGTTCGGATCAAGACATGGCAAGGTGGGTACGGAGCCGTAAGAGGAATAACATTTAGCAACATTCACATGGACAACGTTAGGAATCCCATCATTATCGACCAATTCTACTGTCTAACCAAAGATTGCACCAACCAAACCTCAGCTGTCTCAATATCAGACATTGAATACACAAACATCAAAGGAACATACGACATTCGAAGCTCACCTATGCACCTCGCCTGCAGCGACGCCGTCCCGTGTACCAACCTAACGTTGTCGGAGATCGAGCTCCTTCCTGCTCAAGGAGATATAGTATTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACACTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATCAAATTAAACAATGACAATGAGTATTGTTGATCCTTACCACAACATACAATCATATGTATATAAAATAATCACGAATGTAGTTGAAACGAGGCCCGATCAAGTTTATATGTCATGTCACACGATACCACGAAAAAACAACTCCTATTAAGTAGTTGATTTATAGCTACGTTATATT

mRNA sequence

AGATTATTGAAACTAGATTTGGGACCAAAGTGAGGCATGGTTATGGGAAGAACATGGGAGGCTTATCTCCAATCCCACTGCCCTTTTCTTCTTCCTTTTTCTCAAACACTCAATGCCACTCTCCATTTTGTGTATTAAAGGTCAATGCCTACATGAATTTGGTCGGTTCCACTGCTCAAATAATCAATCCAAAAGTAATAATTTCAACATCAACCCTTTGATTCACCTTCCACTTTCACTTAAAGTTGTGTTTTTTCTCTCATTATATAGCCAAAGATTCAGCCTTTCTTGCTTTGTGTTCATCCCATGTTGAAATACCCTCTTGGGTTCTTGAGAAATCCAAGTAATTTCGTTGAAGAAAGTGTTTTTTGAGTGTTTTGAGACATACCCATCTCAAAAAATGTCTAATTTTGTGTTGTCATTGTCATTTTGTGTTACTTTTTTGTGTTGTCTTCTTTGTTCTTCTCAAGCTCGATGGCATCATCATTCCAAGCAAAAACACTCTCACCATCATAAACGTAGCTACATTTCACATCCTCCTACTCCTTCTCCTTCACCGGGCGATGGCGATTACATTAACGAAACGAATATCTTCGACGTACGAGGCTTTGGTGCTGTCGGCGATGGCGTTGCTGACGACACCGAGGCGTTTAAGATGGCTTGGGATACGGCGTGCCAGAGTGACAATGCTGCCGTCGTGCTCGTTCCTTATGGCTACTCGTTTATGATTCAATCAACAATCTTTACTGGCCCTTGCCATGGTCATCTCATGTTGTGGGTTGATGGCACGTTGATGCCACCGGATGGCCCTGATGCCTGGCCGAGGAATGCTAGTCGCCGGCAGTGGTTGGTGTTCTATAGAATTGATGGGATGTCCTTAGAGGGTGGAGGTGTGGTTGATGGGCGAGGTCAAAAATGGTGGGATCTTCCTTGCAAACCACACAAGGGACCAAATGGAACCACGTTACCAGGACCGTGTGACAGCCCAATTGCTATAAGGTTCTTCATGAGCTCGAATTTGACTGTCCAAGGACTTAAATTCACAAACAGTCCTCAGTTTCACTTCAGATTCGACGGTTGCAAGAATGTTCATATAGAGTCGATTCATATAAAGTCTCCGGGTCTTAGTCCCAACACTGATGGTATCCACATCGAGAACACAAATGGCGTCGGGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCGGGTTCATATGATGTCGATATCCGGAACATCACTTGTGGACCAAGTCATGGAATTAGTATCGGAAGTCTTGGTGTTCGAAACTCGAGAGCTTGTGTTACAAACATAACGGTCAGGGACTCGGTGATCAAAGACTCAGACAACGGTGTTCGGATCAAGACATGGCAAGGTGGGTACGGAGCCGTAAGAGGAATAACATTTAGCAACATTCACATGGACAACGTTAGGAATCCCATCATTATCGACCAATTCTACTGTCTAACCAAAGATTGCACCAACCAAACCTCAGCTGTCTCAATATCAGACATTGAATACACAAACATCAAAGGAACATACGACATTCGAAGCTCACCTATGCACCTCGCCTGCAGCGACGCCGTCCCGTGTACCAACCTAACGTTGTCGGAGATCGAGCTCCTTCCTGCTCAAGGAGATATAGTATTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACACTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATCAAATTAAACAATGACAATGAGTATTGTTGATCCTTACCACAACATACAATCATATGTATATAAAATAATCACGAATGTAGTTGAAACGAGGCCCGATCAAGTTTATATGTCATGTCACACGATACCACGAAAAAACAACTCCTATTAAGTAGTTGATTTATAGCTACGTTATATT

Coding sequence (CDS)

ATGTCTAATTTTGTGTTGTCATTGTCATTTTGTGTTACTTTTTTGTGTTGTCTTCTTTGTTCTTCTCAAGCTCGATGGCATCATCATTCCAAGCAAAAACACTCTCACCATCATAAACGTAGCTACATTTCACATCCTCCTACTCCTTCTCCTTCACCGGGCGATGGCGATTACATTAACGAAACGAATATCTTCGACGTACGAGGCTTTGGTGCTGTCGGCGATGGCGTTGCTGACGACACCGAGGCGTTTAAGATGGCTTGGGATACGGCGTGCCAGAGTGACAATGCTGCCGTCGTGCTCGTTCCTTATGGCTACTCGTTTATGATTCAATCAACAATCTTTACTGGCCCTTGCCATGGTCATCTCATGTTGTGGGTTGATGGCACGTTGATGCCACCGGATGGCCCTGATGCCTGGCCGAGGAATGCTAGTCGCCGGCAGTGGTTGGTGTTCTATAGAATTGATGGGATGTCCTTAGAGGGTGGAGGTGTGGTTGATGGGCGAGGTCAAAAATGGTGGGATCTTCCTTGCAAACCACACAAGGGACCAAATGGAACCACGTTACCAGGACCGTGTGACAGCCCAATTGCTATAAGGTTCTTCATGAGCTCGAATTTGACTGTCCAAGGACTTAAATTCACAAACAGTCCTCAGTTTCACTTCAGATTCGACGGTTGCAAGAATGTTCATATAGAGTCGATTCATATAAAGTCTCCGGGTCTTAGTCCCAACACTGATGGTATCCACATCGAGAACACAAATGGCGTCGGGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCGGGTTCATATGATGTCGATATCCGGAACATCACTTGTGGACCAAGTCATGGAATTAGTATCGGAAGTCTTGGTGTTCGAAACTCGAGAGCTTGTGTTACAAACATAACGGTCAGGGACTCGGTGATCAAAGACTCAGACAACGGTGTTCGGATCAAGACATGGCAAGGTGGGTACGGAGCCGTAAGAGGAATAACATTTAGCAACATTCACATGGACAACGTTAGGAATCCCATCATTATCGACCAATTCTACTGTCTAACCAAAGATTGCACCAACCAAACCTCAGCTGTCTCAATATCAGACATTGAATACACAAACATCAAAGGAACATACGACATTCGAAGCTCACCTATGCACCTCGCCTGCAGCGACGCCGTCCCGTGTACCAACCTAACGTTGTCGGAGATCGAGCTCCTTCCTGCTCAAGGAGATATAGTATTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACACTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATCAAATTAAACAATGACAATGAGTATTGTTGA

Protein sequence

MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC

Homology

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.3e-159
Identity = 264/456 (57.89%), Postives = 329/456 (72.15%), Query Frame = 0


Query: 22  SQARWHHHSKQKHSHHHKRSYISHP----------------PTPSPSPGDGDYINETNIF 81
           ++AR+H+H K +H   H       P                P+P P+PGD D      +F
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSD---SGCVF 84

Query: 82  DVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCHGHLML 141
           DV  FGAVGDG  DDT AF+ AW  AC  + + VVL P G  F I STIF+GPC   L+ 
Sbjct: 85  DVTSFGAVGDGSCDDTAAFQDAWKAACAVE-SGVVLAPEGGVFKITSTIFSGPCKPGLVF 144

Query: 142 WVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKPHKGPN 201
            +DG LMPPDGP+ WP   ++ QWLVFYR+DG +  G G V+G GQKWWDLPCKPH+GP+
Sbjct: 145 QLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPD 204

Query: 202 GTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGLSPN 261
           G++  GPC SP  IRFFMS+N+ V+GL+  NSPQFH +FDGC+ V I  I I SP LSPN
Sbjct: 205 GSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPN 264

Query: 262 TDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRA 321
           TDGIH+ NT  VGIYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+A
Sbjct: 265 TDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQA 324

Query: 322 CVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLTKDC 381
           CV+NITVR++VI+DSDNG+R+KTWQGG G+V  + F NI M+NV N II+DQ+YC +KDC
Sbjct: 325 CVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDC 384

Query: 382 TNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDIVLDPF 441
            N+TSAV + D+EY NIKGTYD+RS P+H ACSD V CTN+T+SE+ELLP +G++V DPF
Sbjct: 385 RNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPF 444

Query: 442 CWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 462
           CWNAYG  +TLTIPP+ CL +G P   +  + N  C
Sbjct: 445 CWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 3.5e-93
Identity = 171/385 (44.42%), Postives = 231/385 (60.00%), Query Frame = 0


Query: 64  IFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCHGHL 123
           +  V  FGA G+GV DDT+AF  AW TAC S     +LVP  Y+ +++    +GPC   L
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111

Query: 124 MLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKPHKG 183
            L + GT++ P+ PD W    +RR+WL F+ +  +++EGGG V+G GQ+WW+  CK H  
Sbjct: 112 TLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNH 171

Query: 184 PNGTTLPGPC-DSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGL 243
            N      PC  +P A+ F    N+ V+ L   +S Q H     C+ V I  + + +P  
Sbjct: 172 SN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPAT 231

Query: 244 SPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRN 303
           SPNTDGIHI  + G+ I N+ +  GDDC+SI   S  + I NI CGP HGISIGSLG   
Sbjct: 232 SPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSK 291

Query: 304 SRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC-L 363
           S   V +ITV  ++I D+ NGVRIKTWQGG G V  I F NI M+NV NPIIIDQ+YC  
Sbjct: 292 SWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDS 351

Query: 364 TKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDIV 423
            K C NQTSA+SI +I + +++GT       + ++CSD+ PC N+ L +I+L P+ GD  
Sbjct: 352 RKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGF 411

Query: 424 LDPFCWNAYGDLQTLTIPPVSCLQE 447
            + FCW AYG       PP  CL +
Sbjct: 412 TESFCWEAYGSSSGQVYPP-PCLSD 426

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.1e-83
Identity = 162/359 (45.13%), Postives = 216/359 (60.17%), Query Frame = 0


Query: 63  NIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCHGH 122
           NIF+V  +GAVGDG  D TEAF  AW  AC+   +A++LVP    F++ +  F GPC  H
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKP-SAMLLVPGNKKFVVNNLFFNGPCQPH 116

Query: 123 LMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKPHK 182
               VDG +     P +W  N   R WL F ++ G +L G GV+DG+G++WW   C   K
Sbjct: 117 FTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC---K 176

Query: 183 GPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGL 242
             NG  +    D P AI+F  S+ L +QGLK  NSP+FH  F  C+ V I  I I +P  
Sbjct: 177 WVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRD 236

Query: 243 SPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRN 302
           SPNTDGI I  +    +  + I  GDDCV+IG+GS ++ I ++ CGP HGISIGSLG  N
Sbjct: 237 SPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGREN 296

Query: 303 SRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLT 362
           SRA V+ + V  +   D+ NG+RIKTWQGG G    I + N+ M N  NPI+I+QFYC +
Sbjct: 297 SRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTS 356

Query: 363 KD-CTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 421
              C NQ SAV I D+ Y NI+GT    ++ + L CSD++PC ++ LS+I L    G I
Sbjct: 357 ASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.4e-81
Identity = 158/356 (44.38%), Postives = 216/356 (60.67%), Query Frame = 0


Query: 64  IFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCHGHL 123
           +F+V  +GAVGDG  D TEAF   W+ AC+   +AV+LVP    F + + +F GPC  HL
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKK-ASAVLLVPANKKFFVNNLVFRGPCQPHL 117

Query: 124 MLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKPHKG 183
              VDGT++    P  W  +   + WL F ++   +L G GV+DG+GQ+WW   CK    
Sbjct: 118 SFKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV--- 177

Query: 184 PNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGLS 243
            NG T+    + P AI+   S ++TV+ L   NSP+FH  F  C+ V I+ + IK+P  S
Sbjct: 178 VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDS 237

Query: 244 PNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 303
           PNTDGI I  +    I   VI  GDDC++IG+GS ++ I+++ CGP HGISIGSLG  NS
Sbjct: 238 PNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNS 297

Query: 304 RACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLTK 363
           RA V+++ V  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC + 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 364 D-CTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQG 419
             C NQ SAV I  + Y NI GT    ++ + L CSD+VPCT + LS + L    G
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 9.9e-80
Identity = 154/358 (43.02%), Postives = 215/358 (60.06%), Query Frame = 0


Query: 64  IFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCHGHL 123
           +F+V  +GAVGDG  D T+AF+  W+ AC +  +AV LVP    F++ + +F GPC  H 
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAAC-NKLSAVFLVPANKKFVVNNLVFYGPCQPHF 118

Query: 124 MLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKPHKG 183
              VDGT+     P  W  +   + W+ F R+   +L G GV+DG+G +WW   CK    
Sbjct: 119 SFKVDGTIAAYPDPAKWKNS---KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKT--- 178

Query: 184 PNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGLS 243
            NG T+      P AI+   S ++TV+ L  TNSP+FH  F  C  V I+ I IK+P  S
Sbjct: 179 INGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDS 238

Query: 244 PNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 303
           PNTDGI I  +    I    I  GDDCV++G+GS ++ I+++TCGP HG+SIGSLG  NS
Sbjct: 239 PNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNS 298

Query: 304 RACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLT- 363
           R+ V+ + +  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC + 
Sbjct: 299 RSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSA 358

Query: 364 KDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 421
             C NQ SAV I D+ + NI GT    ++ + L CSD+VPC+N+ LS + L    G +
Sbjct: 359 AACKNQRSAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

[Close]

BLAST of Cp4.1LG12g06450 vs. NCBI nr
Match: XP_023547817.1 (polygalacturonase At1g48100 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60
           MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN
Sbjct: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120
           ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180
           GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240
           HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360
           RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420
           LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of Cp4.1LG12g06450 vs. NCBI nr
Match: KAG6575738.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 459/461 (99.57%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60
           MSNFVLSLSF VTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120
           ETNIFDVRGFGAVGDGVADDTEAFKMAWD ACQSDNAAVVLVPYGYSFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180
           GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240
           HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360
           RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420
           LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of Cp4.1LG12g06450 vs. NCBI nr
Match: XP_022992012.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 967 bits (2499), Expect = 0.0
Identity = 457/461 (99.13%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60
           MSNFVLSLSF VTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120
           ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSD+AAVVLVPYGYSFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180
           GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240
           HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTN VGIYNS+IYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360
           RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420
           LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of Cp4.1LG12g06450 vs. NCBI nr
Match: XP_022953772.1 (polygalacturonase At1g48100 [Cucurbita moschata])

HSP 1 Score: 966 bits (2497), Expect = 0.0
Identity = 457/461 (99.13%), Postives = 458/461 (99.35%), Query Frame = 0


Query: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60
           MSNFVLSLSF VTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120
           ETNIFDVRGFGAVGDGVADDTEAFKMAWD ACQSD+AAVVLVPYGYSFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180
           GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240
           HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           G SPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360
           RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420
           LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of Cp4.1LG12g06450 vs. NCBI nr
Match: KAG7014289.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 456/461 (98.92%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60
           MSNFVLSLSF VTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120
           ETNIFDVRGFGA+GDGVADDTEAFKMAWD ACQSD+AAVVLVPYGYSFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAIGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180
           GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRG+KWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGKKWWDLPCKP 180

Query: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240
           HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360
           RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420
           LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy TrEMBL
Match: A0A6J1JNI5 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=1)

HSP 1 Score: 967 bits (2499), Expect = 0.0
Identity = 457/461 (99.13%), Postives = 459/461 (99.57%), Query Frame = 0


Query: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60
           MSNFVLSLSF VTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120
           ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSD+AAVVLVPYGYSFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180
           GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240
           HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           GLSPNTDGIHIENTN VGIYNS+IYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360
           RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420
           LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy TrEMBL
Match: A0A6J1GP58 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 SV=1)

HSP 1 Score: 966 bits (2497), Expect = 0.0
Identity = 457/461 (99.13%), Postives = 458/461 (99.35%), Query Frame = 0


Query: 1   MSNFVLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60
           MSNFVLSLSF VTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN
Sbjct: 1   MSNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGDGDYIN 60

Query: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCH 120
           ETNIFDVRGFGAVGDGVADDTEAFKMAWD ACQSD+AAVVLVPYGYSFMIQSTIFTGPCH
Sbjct: 61  ETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFTGPCH 120

Query: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180
           GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP
Sbjct: 121 GHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKP 180

Query: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240
           HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP
Sbjct: 181 HKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSP 240

Query: 241 GLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300
           G SPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV
Sbjct: 241 GFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGV 300

Query: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360
           RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC
Sbjct: 301 RNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC 360

Query: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420
           LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI
Sbjct: 361 LTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI 420

Query: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC
Sbjct: 421 VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 419/468 (89.53%), Postives = 442/468 (94.44%), Query Frame = 0


Query: 1   MSNFVLSLSFCV--TFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPT----PSPSPG 60
           MS+FVL LSF +  +F+ CLL SSQARWHHH+KQKHSHHH+ SYISHPP+    PS SP 
Sbjct: 1   MSSFVL-LSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPD 60

Query: 61  DGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQS-DNAAVVLVPYGYSFMIQST 120
           DGDYIN+TNIFDVR FGA+GDG+ DDTEAFKMAWDTACQS D++AV+LVPYG+SFMIQST
Sbjct: 61  DGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKW 180
           IFTGPCHG+LML VDGTLMPPDGPDAWP+N+SRRQWLVFYRIDGMSLEGGGV+DGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIE 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLK TNSPQFHFRFDGCK VHI+
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGV IYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIEL 420
           IIDQFYCLTKDCTNQTSAVS+SDI+YTNIKGTYDIRS PMH ACSD+VPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI  NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 878 bits (2269), Expect = 0.0
Identity = 418/468 (89.32%), Postives = 441/468 (94.23%), Query Frame = 0


Query: 1   MSNFVLSLSFCV--TFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPT----PSPSPG 60
           MS+FVL LSF +  +F+ CLL SSQARWHHH+KQKHSHHH+  YISHPP+    PS SP 
Sbjct: 1   MSSFVL-LSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPD 60

Query: 61  DGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQS-DNAAVVLVPYGYSFMIQST 120
           DGDYIN+TNIFDVR FGA+GDG+ DDTEAFKMAWDTACQS D++AV+LVPYG+SFMIQST
Sbjct: 61  DGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKW 180
           IFTGPCHG+LML VDGTLMPPDGPDAWP+N+SRRQWLVFYRIDGMSLEGGGV+DGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIE 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLK TNSPQFHFRFDGCK VHI+
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGV IYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIEL 420
           IIDQFYCLTKDCTNQTSAVS+SDI+YTNIKGTYDIRS PMH ACSD+VPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI  NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

[Close]

BLAST of Cp4.1LG12g06450 vs. ExPASy TrEMBL
Match: A0A0A0KBA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1)

HSP 1 Score: 869 bits (2246), Expect = 0.0
Identity = 415/468 (88.68%), Postives = 438/468 (93.59%), Query Frame = 0


Query: 1   MSNFVLSLSFCV--TFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPT----PSPSPG 60
           MS+FVL LSF +  +F+ CLL SSQARWHHH+KQKHSH H+ SYISHPP+    PS SP 
Sbjct: 1   MSSFVL-LSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPD 60

Query: 61  DGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQS-DNAAVVLVPYGYSFMIQST 120
           DG YIN+TNIFDVR FGA+GDG+ DDTEAFKMAWDTACQS D++ V+LVPYG+SFMIQST
Sbjct: 61  DGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKW 180
           IFTGPCHG+LML VDGTLM PDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIE 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTV GLK TNSPQFHFRFDGCK VHI+
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGV IYNSVI NGDDCVSIGSGSYD+DIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIEL 420
           IIDQFYCLTKDCTNQTSAVS+SDI+YTNIKGTYDIRS PMH ACSD+VPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+  NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

[Close]

BLAST of Cp4.1LG12g06450 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 645.2 bits (1663), Expect = 4.0e-185
Identity = 310/468 (66.24%), Postives = 367/468 (78.42%), Query Frame = 0


Query: 12  VTFLCCLLCSSQARWHHHSKQKHSH----HHK------RSYISHPPTP------------ 71
           +T +  L+  S+AR HHH K+KH H    HH        S IS PPTP            
Sbjct: 15  MTLILTLMSLSEARNHHH-KEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSL 74

Query: 72  SPSPGDGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDN--AAVVLVPYGYS 131
            PSP D    +   I++VR +GAVGDG  DDTEAFK AWD++C ++N   +V+LVPYGY+
Sbjct: 75  PPSPSDDPADDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYT 134

Query: 132 FMIQSTIFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVD 191
           FMIQSTIFTGPC  +    VDGT++ PDGP++WP N S+RQWLVFYR++GM+L+G GV+D
Sbjct: 135 FMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVID 194

Query: 192 GRGQKWWDLPCKPHKGPNGTTL-PGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDG 251
           GRGQKWWDLPCKPH+  N + +  GPCDSPIA+RFFMSSNL V+GL+  NSPQFHFRFDG
Sbjct: 195 GRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDG 254

Query: 252 CKNVHIESIHIKSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNIT 311
           C+ VH+ES+HI +P LSPNTDGIHIEN+N V IYNS+I NGDDCVSIGSGSYDVDIRN+T
Sbjct: 255 CQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLT 314

Query: 312 CGP-SHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIH 371
           CGP  HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+NIH
Sbjct: 315 CGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIH 374

Query: 372 MDNVRNPIIIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTN 431
           +D+VRNPIIIDQ+YC+TKDC N+TSAV +SDI Y  IKGTYDIRS PMH  CSDAVPCTN
Sbjct: 375 VDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTN 434

Query: 432 LTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIK 454
           LTLS+IELLPA+G+IVLDPFCWNAYG  + L+IPPV CL    P  ++
Sbjct: 435 LTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGLQ 481

[Close]

BLAST of Cp4.1LG12g06450 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 571.6 bits (1472), Expect = 5.6e-163
Identity = 274/460 (59.57%), Postives = 341/460 (74.13%), Query Frame = 0


Query: 5   VLSLSFCVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPGD-------GD 64
           + +L   +TF   L   ++AR HH + Q             PP+PSP+P D         
Sbjct: 12  ITTLILIITF-SLLSYGTEARLHHQASQ-------------PPSPSPNPNDPSKSPSRSQ 71

Query: 65  YINETNIFDVRGFGAVGDGVADDTEAFKMAWDTAC---QSDNAAVVLVPYGYSFMIQSTI 124
            ++   ++DVR +GAVG+GVADDT +FK AWD+AC   +++ A+V+ VPYG++FMI+STI
Sbjct: 72  DLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACSNNKNNTASVLHVPYGFTFMIRSTI 131

Query: 125 FTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWW 184
           FTGPC  +    VDGT++P DGP +WP + ++RQWL FYRI+GM+L+G GV+DGRGQ WW
Sbjct: 132 FTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWW 191

Query: 185 DLPCKPH-KGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIE 244
           DLPCKPH +  N T L GPC+SP A+RFFMSSN+ V+GL   NSPQ H + DGC  VHI 
Sbjct: 192 DLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHIN 251

Query: 245 SIHIKSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGP-SHG 304
           S+ I SP  SPNTDGIHIEN+N V IYNSVI NGDDCVSIG G+YD+DIRNITCGP  HG
Sbjct: 252 SLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHG 311

Query: 305 ISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNP 364
           ISIGSLG +NS ACV+N+TVRDS IK S+NGVRIKTWQGG G+V G+TF NIH+D VRNP
Sbjct: 312 ISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNP 371

Query: 365 IIIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIE 424
           IIIDQ+YC TK C N+TSAV ++DI Y +IKGTYDIRS PMH  CS+ VPCTNLTLS IE
Sbjct: 372 IIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIE 431

Query: 425 LLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI 453
           LLP++ DIV+ PFCWNAYG     ++P +SCL+    +S+
Sbjct: 432 LLPSKEDIVVGPFCWNAYGITDEFSVPLISCLKSNPSTSL 457

[Close]

BLAST of Cp4.1LG12g06450 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 564.3 bits (1453), Expect = 9.0e-161
Identity = 264/456 (57.89%), Postives = 329/456 (72.15%), Query Frame = 0


Query: 22  SQARWHHHSKQKHSHHHKRSYISHP----------------PTPSPSPGDGDYINETNIF 81
           ++AR+H+H K +H   H       P                P+P P+PGD D      +F
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSD---SGCVF 84

Query: 82  DVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVLVPYGYSFMIQSTIFTGPCHGHLML 141
           DV  FGAVGDG  DDT AF+ AW  AC  + + VVL P G  F I STIF+GPC   L+ 
Sbjct: 85  DVTSFGAVGDGSCDDTAAFQDAWKAACAVE-SGVVLAPEGGVFKITSTIFSGPCKPGLVF 144

Query: 142 WVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCKPHKGPN 201
            +DG LMPPDGP+ WP   ++ QWLVFYR+DG +  G G V+G GQKWWDLPCKPH+GP+
Sbjct: 145 QLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPD 204

Query: 202 GTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGLSPN 261
           G++  GPC SP  IRFFMS+N+ V+GL+  NSPQFH +FDGC+ V I  I I SP LSPN
Sbjct: 205 GSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPN 264

Query: 262 TDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRA 321
           TDGIH+ NT  VGIYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+A
Sbjct: 265 TDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQA 324

Query: 322 CVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLTKDC 381
           CV+NITVR++VI+DSDNG+R+KTWQGG G+V  + F NI M+NV N II+DQ+YC +KDC
Sbjct: 325 CVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDC 384

Query: 382 TNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDIVLDPF 441
            N+TSAV + D+EY NIKGTYD+RS P+H ACSD V CTN+T+SE+ELLP +G++V DPF
Sbjct: 385 RNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPF 444

Query: 442 CWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 462
           CWNAYG  +TLTIPP+ CL +G P   +  + N  C
Sbjct: 445 CWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

[Close]

BLAST of Cp4.1LG12g06450 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 549.7 bits (1415), Expect = 2.3e-156
Identity = 251/410 (61.22%), Postives = 321/410 (78.29%), Query Frame = 0


Query: 42  YISHPPTPSPSPGDGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQS--DNAAV 101
           Y+S  P P+P+  D D I  T +FDV  FGA+GD   DDT AFKMAWD AC S    +A+
Sbjct: 26  YLSPSPAPNPAYNDNDNIAPT-VFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSAL 85

Query: 102 VLVPYGYSFMIQSTIFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMS 161
           +LVPY + F+++ T F GPC  +L+L +DG ++ PDGP +WP N  +RQW++FYR++G+S
Sbjct: 86  LLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYRVNGLS 145

Query: 162 LEGGGVVDGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQ 221
           ++G GV++GRGQKWW+LPCKPHKG NGTT  GPCDSP+AIR F SS + +QG+ F NS Q
Sbjct: 146 IQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQ 205

Query: 222 FHFRFDGCKNVHIESIHIKSPGLSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYD 281
           FH RFD C +V ++S+ IK+P  SPNTDGIHIENT+ V I NS+I NGDDC+SIG+G ++
Sbjct: 206 FHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFN 265

Query: 282 VDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGI 341
           VDI+N+TCGPSHGISIGSLGV NS+A V+NITV +S I +SDNGVRIKTWQGG G+V  I
Sbjct: 266 VDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRI 325

Query: 342 TFSNIHMDNVRNPIIIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSD 401
            FSNI M NVRNPI+IDQ+YC T +C NQTSAV ISD+ Y NIKGTYD+RS P+H  CSD
Sbjct: 326 VFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSD 385

Query: 402 AVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLP 450
           +VPCTNLTL+E++L P++G  + +PFCWNAYG ++ +T+PPV CL +  P
Sbjct: 386 SVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

[Close]

BLAST of Cp4.1LG12g06450 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 377.1 bits (967), Expect = 2.0e-104
Identity = 182/447 (40.72%), Postives = 275/447 (61.52%), Query Frame = 0


Query: 42  YISHPPTPSPSPGDGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDNAAVVL 101
           ++  PPTP P       + ++  F+V  FGA GDG++DDT+AF+ AW +AC+ + A+ ++
Sbjct: 104 HVQPPPTPLPL----QPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVE-ASTMI 163

Query: 102 VPYGYSFMIQSTIFTGP-CHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSL 161
           +P  Y F++    F+GP C  +++  ++G ++ P   ++W        W+ F ++ G+++
Sbjct: 164 IPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDTESW--GGGLMWWIEFTKLSGITI 223

Query: 162 EGGGVVDGRGQKWW-------------------------DLPCKPHKGPNGTTLPGPCDS 221
           +G GV+DGRG  WW                         + P  P +      +P     
Sbjct: 224 QGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPS--IK 283

Query: 222 PIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKSPGLSPNTDGIHIENTN 281
           P A+RF+ S ++TV G+   NSPQ H +FD C  V +  +++ SPG SPNTDGIH++NT 
Sbjct: 284 PTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTR 343

Query: 282 GVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDS 341
            V I+ + +  GDDC+SI +G  +V + N+ CGP HGISIGSLG  +++ACV+NITVRD 
Sbjct: 344 DVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDV 403

Query: 342 VIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLTKDCTNQTSAVSIS 401
           V+ ++  GVRIKTWQGG G+V+GI FSNI +  V+ PI+IDQFYC    C N TSAVS+ 
Sbjct: 404 VMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVE 463

Query: 402 DIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGDI-VLDPFCWNAYGDLQ 461
            + Y  I+GTY ++  P+H ACSD+ PC ++ LS IEL P Q    + DPFCW  +G+L 
Sbjct: 464 GVTYEKIRGTYTVK--PVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELN 523

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g06450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	1.3e-159	57.89	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	3.5e-93	44.42	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	1.1e-83	45.13	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	1.4e-81	44.38	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q9FY19	9.9e-80	43.02	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]

BLAST of Cp4.1LG12g06450 vs. NCBI nr


Match Name	Identity	Description
XP_023547817.1	100.00	polygalacturonase At1g48100 [Cucurbita pepo subsp. pepo]	[more]
KAG6575738.1	99.57	Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022992012.1	99.13	polygalacturonase At1g48100 [Cucurbita maxima]	[more]
XP_022953772.1	99.13	polygalacturonase At1g48100 [Cucurbita moschata]	[more]
KAG7014289.1	98.92	Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cp4.1LG12g06450 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1JNI5	99.13	polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=...	[more]
A0A6J1GP58	99.13	polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 S...	[more]
A0A5D3BFC7	89.53	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00...	[more]
A0A1S3CFX8	89.32	polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV...	[more]
A0A0A0KBA7	88.68	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1	[more]

BLAST of Cp4.1LG12g06450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02460.1	4.0e-185	66.24	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	5.6e-163	59.57	Pectin lyase-like superfamily protein	[more]
AT1G48100.1	9.0e-161	57.89	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	2.3e-156	61.22	Pectin lyase-like superfamily protein	[more]
AT1G60590.1	2.0e-104	40.72	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 336..357 e-value: 710.0 score: 8.3 coord: 277..297 e-value: 6600.0 score: 1.4 coord: 307..328 e-value: 1.1 score: 18.2 coord: 254..275 e-value: 2600.0 score: 4.3 coord: 227..253 e-value: 23.0 score: 13.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 59..452 e-value: 4.1E-121 score: 406.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 92..413 e-value: 1.6E-88 score: 296.9
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 284..297
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..56
None	No IPR available	PANTHER	PTHR31375:SF119	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 6..450
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 6..450
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 62..451

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g06450.1	Cp4.1LG12g06450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004650	polygalacturonase activity