Cp4.1LG12g06410 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG12g06410 vs. ExPASy Swiss-Prot
Match: Q10B19 (tRNA:m(4)X modification enzyme TRM13 OS=Oryza sativa subsp. japonica OX=39947 GN=TRM13 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.5e-64
Identity = 125/244 (51.23%), Postives = 162/244 (66.39%), Query Frame = 0

Query: 17  RCQFWLPKKNRFCANSPINDSKFCGNH--SSRADT-----QWVPCPVDPSHSVLRENLEW 76
           RC FWLP K R CAN+P+  S++CGNH   S +D      + VPCPVDPSH+VL ENLE 
Sbjct: 21  RCHFWLPNKRRHCANTPLPTSQYCGNHLPDSASDAGAPFRRLVPCPVDPSHTVLEENLEA 80

Query: 77  HVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGA 136
           HV +CPL KQ  +LA QPFY KGIN+G  +              ++S  KR +V+ ++  
Sbjct: 81  HVGKCPLKKQAAALAAQPFYSKGINSGGGEG----------GGGVTSAAKRALVHKLTKD 140

Query: 137 EFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNME 196
           E   L+ KI+  H      ++DS+ + +AC+ W++ ++DRK+P+QEKHV QQASI+GNME
Sbjct: 141 ELRALIEKIKLAHASAAMAMRDSFLVTDACDNWMRNQVDRKVPYQEKHVTQQASIIGNME 200

Query: 197 EFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKS 254
            FG+L+   GGE  E      N    APAV+EFGAGRGYLTQMLADCYGI+ V LVER+S
Sbjct: 201 AFGLLQK--GGEVAE-----ENGVKNAPAVVEFGAGRGYLTQMLADCYGIRNVFLVERRS 247

BLAST of Cp4.1LG12g06410 vs. ExPASy Swiss-Prot
Match: Q8BYH3 (tRNA:m(4)X modification enzyme TRM13 homolog OS=Mus musculus OX=10090 GN=Trmt13 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.5e-51
Identity = 158/501 (31.54%), Postives = 226/501 (45.11%), Query Frame = 0

Query: 7   SEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQ----WVPCPVDPSHSV 66
           S  +G  +  RC +++ KK RFC        +FCG H+  A+ +     + CP+DP H+V
Sbjct: 8   SPSIGFPIDGRCNYFVEKKKRFCRMVAAAGKRFCGEHAGSAEEENTRKRILCPLDPKHTV 67

Query: 67  LRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNI 126
             + L  H+K+C     ++      F+ + INAG +D     E    FS S+S E   N+
Sbjct: 68  YEDQLAKHLKKC----NSREKPKPDFFIQDINAGLKDETEIPEQLVPFS-SLSEEQLENL 127

Query: 127 VYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQA 186
           +         +L      L+      I     + +A N    G+         KH+ QQA
Sbjct: 128 I--------KKLRKASEGLNSTHEDHIMSHPALHDALNDPRNGDC------AVKHLKQQA 187

Query: 187 SILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQ----MLADCYG 246
           SILGN+E+  +L          G R C          +EFGAG+G L+      L D   
Sbjct: 188 SILGNIEKLKLL----------GPRRC---------FVEFGAGKGKLSHWVDIALKDAEN 247

Query: 247 IKRVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHN--PYLAIGKHL 306
           +   +LVER + + K D   R+K+S V ERL+IDI+ L LN V  LR    P + IGKHL
Sbjct: 248 V-HFILVERVTTRFKVDGKHRKKDS-VFERLQIDIQHLCLNRVPVLREGRLPVVGIGKHL 307

Query: 307 CGPATDLALRCCL------------------------TKQSNHVNTEQC-GDRPK----L 366
           CG ATD+ALRC +                         K+SN +  E    D P+    +
Sbjct: 308 CGVATDVALRCLVETYAASFEEKDEEPLAKRIKNDKTEKESNTLAKEGSEKDVPETWTPV 367

Query: 367 RGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAV-DANHSEDISDGADL 426
            G+ IA CCHH C W+HY  K Y   LG+   EF      +SWA      S + SD    
Sbjct: 368 AGIVIALCCHHRCDWRHYVGKEYFKALGLGAVEFYYFQRMSSWATCGMRTSLEGSDVTPE 427

Query: 427 KTFLQSSENE-----GDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRLMWLKECGL 463
           +   Q  ENE     GD +     + +   +   E+  +G +CK +ID GRL +L++ G 
Sbjct: 428 RKDAQRDENEEHDDGGDRLTDGNTDSLPGILTVEEKKKIGHLCKLLIDQGRLQYLQQKGF 468

BLAST of Cp4.1LG12g06410 vs. ExPASy Swiss-Prot
Match: Q9NUP7 (tRNA:m(4)X modification enzyme TRM13 homolog OS=Homo sapiens OX=9606 GN=TRMT13 PE=1 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.9e-46
Identity = 150/500 (30.00%), Postives = 215/500 (43.00%), Query Frame = 0

Query: 11  GLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQ----WVPCPVDPSHSVLREN 70
           G     RC +++ KK RFC        +FCG H+  A+ +     + CP+DP H+V  + 
Sbjct: 12  GFPAEGRCGYYVEKKKRFCRMVVAAGKRFCGEHAGAAEEEDARKRILCPLDPKHTVYEDQ 71

Query: 71  LEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGM 130
           L  H+K+C     ++      FY + INAG  D     E     S S+S E    ++   
Sbjct: 72  LAKHLKKC----NSREKPKPDFYIQDINAGLRDETEIPEQLVPIS-SLSEEQLEKLI--- 131

Query: 131 SGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILG 190
                 +L      L+  +   I     + +A N    G+         KH+ QQASILG
Sbjct: 132 -----KKLRKASEGLNSTLKDHIMSHPALHDALNDPKNGD------SATKHLKQQASILG 191

Query: 191 NMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQ----MLADCYGIKRV 250
           N+E   +L          G R C          +EFGAG+G L+      L D   +   
Sbjct: 192 NIENLKLL----------GPRRC---------FVEFGAGKGKLSHWVDIALKDAEKV-HF 251

Query: 251 LLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHN--PYLAIGKHLCGPA 310
           +LVE+ + + K D   R+K S V ERL+IDI+ L LN +  LR    P + IGKHLCG A
Sbjct: 252 ILVEKVTTRFKVDGKHRKKNS-VFERLQIDIQHLCLNKIPVLREEKLPVVGIGKHLCGMA 311

Query: 311 TDLALRC-------------------------------CLTKQSNHVNTEQCGDRPKLRG 370
           TDLALRC                                L K+ N  N  +  +   + G
Sbjct: 312 TDLALRCLVETYAASFEERNEEPLAKRIKNDKTEKEIYTLAKEGNEKNVPEKWN--PVAG 371

Query: 371 LAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVDA--NHSEDISDGADLK 430
           + IA CCHH C W+HY  K Y   LG+   EF      +SWA       S + S+    +
Sbjct: 372 IVIALCCHHRCDWRHYVGKEYFRALGLGAVEFHYFQRMSSWATCGMRKTSLETSNSTTKR 431

Query: 431 TFLQSSENEGDGMNGSTVEDIARHMNP-----VERAVLGFMCKEIIDMGRLMWLKECGLE 463
              Q+ ++E     G  + D      P      E+  +G +CK +ID GR+ +L++ G  
Sbjct: 432 QDNQNDDSEEHDDGGYRITDDGADCLPGLLSVEEKKKIGHLCKLLIDQGRIQYLQQKGFS 469

BLAST of Cp4.1LG12g06410 vs. ExPASy Swiss-Prot
Match: Q6CP50 (tRNA:m(4)X modification enzyme TRM13 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=TRM13 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.6e-35
Identity = 134/492 (27.24%), Postives = 219/492 (44.51%), Query Frame = 0

Query: 1   MKAVPVSEELGLEMASR--CQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDP 60
           M AV VS   G   A R  C+F+L KK R C  +    S+FC  HS+  D+  VPCP+DP
Sbjct: 1   MMAVTVSGNTGQSTAERLQCEFFLVKKKRQCGMTRRAGSQFCSEHST--DSDRVPCPLDP 60

Query: 61  SHSVLRENLEWHVKRCPLLKQTQSLAVQ----PFYQKGINAGEEDSISSDELASGFSDSI 120
           SH+V    +  H+++C   +   S   +    P++++G+N+          +++G SDS 
Sbjct: 61  SHTVCLSKMRVHMRKCNKFRHDVSYQAKQREIPWFKEGLNS----------ISNGKSDSK 120

Query: 121 SSEMKRNIVYGMSGAEFHRLLAKIRALHGLICKDIQDS--YRIKEACNMWIKGEIDRKIP 180
            ++                L+  +  +  +   +  D     + E  N  ++     K  
Sbjct: 121 PAD--------------ETLVKSVSLIQKIFQNEFGDEPPLPLIEKQNELLERTERYKTL 180

Query: 181 FQEKHVLQQASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQM 240
              KH  QQ+S++ +++E    + +   E C  N++           IE G GR   ++ 
Sbjct: 181 VNRKHARQQSSLIQHLKE---SKLWPSLESCAVNKTL--------EYIELGCGRAEFSRY 240

Query: 241 LADCYGIKR----------------VLLVERKSYKLKADRSLRQK--ESLVLERLRIDIE 300
           +     + +                  L++R S +L+ D          + + R +IDI+
Sbjct: 241 VNIATNLDQKEHSHEKPEYKAVAPSFTLIDRASQRLRFDNKFSSDIGTEVQIRREKIDIK 300

Query: 301 DLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIAT 360
           DL L+AV     + Y+AI KHLCG ATDL+LRC L       N+++C  +  LRG+ IA 
Sbjct: 301 DLRLDAVLH-DASQYVAISKHLCGVATDLSLRCLL-------NSDKC--KANLRGILIAM 360

Query: 361 CCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVDANHSEDISDGADLKTFLQ--- 420
           CC H+CQ   Y N+ Y+   G+ K +      ++ +               LK F     
Sbjct: 361 CCRHVCQSSEYVNQEYI--KGLLKRQTDGSMAYSEFF------------QCLKKFCSYCT 420

Query: 421 -SSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRLMWLKECGLETQLVKYVP 463
                + D  +GS  ED    +   ER  +G M + IID GR  +L+  G ET L KY  
Sbjct: 421 CGLRPDMDPNSGS--EDHITKLTHNERQRIGHMARRIIDEGRAQFLQSKGFETVLFKYTD 429

BLAST of Cp4.1LG12g06410 vs. ExPASy Swiss-Prot
Match: Q12383 (tRNA:m(4)X modification enzyme TRM13 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM13 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.3e-34
Identity = 125/503 (24.85%), Postives = 214/503 (42.54%), Query Frame = 0

Query: 17  RCQFWLPKKNRFCANSPINDSKFCGNH------------------SSRADTQWVPCPVDP 76
           +C++++ KK R C  +  + + +C  H                   +  + + VPCP+DP
Sbjct: 20  QCEYFMEKKKRRCGMTRSSQNLYCSEHLNLMKKAANSQVHNKNGSEAEKERERVPCPLDP 79

Query: 77  SHSVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEM 136
           +H+V  + L+ H+K+C   K +     +P+Y+ G N              G +  +SS +
Sbjct: 80  NHTVWADQLKKHLKKCNKTKLSHLNDDKPYYEPGYN--------------GENGLLSSSV 139

Query: 137 KRNIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHV 196
           K +I           L+  I  L+ +   +  D   +++  N  +  +   ++P   KH 
Sbjct: 140 KIDIT-------AEHLVQSIELLYKVFEGESMDELPLRQLNNKLMSLKRFPQLPSNTKHA 199

Query: 197 LQQASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRG----------- 256
           +QQ+S++ N+ + G                   E   +   IEFG GR            
Sbjct: 200 VQQSSLIENLVDAGAF-----------------ERPESLNFIEFGCGRAEFSRYVSLYLL 259

Query: 257 -YLTQMLADCYG--IKRVLLVERKSYKLKADRSLRQKESLV-------------LERLRI 316
             LT + A+  G      +L++R + ++K D+ ++   S +             ++R++I
Sbjct: 260 TQLTSLPAEHSGSNSNEFVLIDRATNRMKFDKKIKDDFSEIKSNSPSKPISCPSIKRIKI 319

Query: 317 DIEDLNLNAVESLRHNP-----YLAIGKHLCGPATDLALRCCLTKQSNHVNTEQCGDRPK 376
           DI DL ++ +  L+  P     Y+ I KHLCG ATDL LRC       H   +  G  PK
Sbjct: 320 DIRDLKMDPI--LKSTPGDDIQYVCISKHLCGVATDLTLRCIGNSSILH-GDDNNGCNPK 379

Query: 377 LRGLAIATCCHHLCQWKHYTNKRYLLELG---------ITKEEFL-AITWFTSWAVDANH 436
           L+ + IA CC H+C +  Y N+ Y+  L          +T E F   +T   SW      
Sbjct: 380 LKAICIAMCCRHVCDYGDYVNRSYVTSLVEKYRAHGSILTYETFFRVLTKLCSWG----- 439

Query: 437 SEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRLMWL 458
               + G    T +    N  +   G+    I       ER  +G M + +ID GRL+++
Sbjct: 440 ----TCGRKPGTAITDIVNVVESFEGAEPYTITIK----ERENIGLMARRVIDEGRLVYV 468

BLAST of Cp4.1LG12g06410 vs. NCBI nr
Match: XP_023547814.1 (tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 470/470 (100.00%), Postives = 470/470 (100.00%), Query Frame = 0

Query: 1   MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSH 60
           MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSH
Sbjct: 1   MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSH 60

Query: 61  SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKR 120
           SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKR
Sbjct: 61  SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKR 120

Query: 121 NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ 180
           NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ
Sbjct: 121 NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ 180

Query: 181 QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK 240
           QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK
Sbjct: 181 QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK 240

Query: 241 RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPA 300
           RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPA
Sbjct: 241 RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPA 300

Query: 301 TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE 360
           TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE
Sbjct: 301 TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE 360

Query: 361 FLAITWFTSWAVDANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVL 420
           FLAITWFTSWAVDANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVL
Sbjct: 361 FLAITWFTSWAVDANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVL 420

Query: 421 GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470
           GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA
Sbjct: 421 GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470

BLAST of Cp4.1LG12g06410 vs. NCBI nr
Match: KAG7014284.1 (tRNA:m(4)X modification enzyme TRM13-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 463/470 (98.51%), Postives = 466/470 (99.15%), Query Frame = 0

Query: 1   MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSH 60
           MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSH
Sbjct: 1   MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSH 60

Query: 61  SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKR 120
           SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASG SD ISSEMKR
Sbjct: 61  SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDGISSEMKR 120

Query: 121 NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ 180
           NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ
Sbjct: 121 NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ 180

Query: 181 QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK 240
           QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK
Sbjct: 181 QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK 240

Query: 241 RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPA 300
           RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRH+PYLAIGKHLCGPA
Sbjct: 241 RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPA 300

Query: 301 TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE 360
           TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE
Sbjct: 301 TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE 360

Query: 361 FLAITWFTSWAVDANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVL 420
           FLAITWFTSWAVDANHSEDISD +D KTFLQSSENEGDGMNGSTVEDIA+HMNPVERAVL
Sbjct: 361 FLAITWFTSWAVDANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIAKHMNPVERAVL 420

Query: 421 GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470
           GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA
Sbjct: 421 GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470

BLAST of Cp4.1LG12g06410 vs. NCBI nr
Match: KAG6575728.1 (tRNA:m(4)X modification enzyme TRM13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 460/470 (97.87%), Postives = 466/470 (99.15%), Query Frame = 0

Query: 1   MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSH 60
           MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPI+DSKFCGNHSSRADTQWVPCPVDPSH
Sbjct: 1   MKAVPVSEELGLEMASRCQFWLPKKNRFCANSPISDSKFCGNHSSRADTQWVPCPVDPSH 60

Query: 61  SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKR 120
           SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISS+ELASG SD ISSEMKR
Sbjct: 61  SVLRENLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSNELASGLSDGISSEMKR 120

Query: 121 NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ 180
           NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ
Sbjct: 121 NIVYGMSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQ 180

Query: 181 QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK 240
           QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK
Sbjct: 181 QASILGNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIK 240

Query: 241 RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPA 300
           RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRH+PYLAIGKHLCGPA
Sbjct: 241 RVLLVERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPA 300

Query: 301 TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE 360
           TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE
Sbjct: 301 TDLALRCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEE 360

Query: 361 FLAITWFTSWAVDANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVL 420
           FLAITWFTSWAVDANHSEDISD +D KTFLQSSENEGDGMNGSTVEDIA+HMNPVERAVL
Sbjct: 361 FLAITWFTSWAVDANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIAKHMNPVERAVL 420

Query: 421 GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470
           GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLL+AKCRNICAA
Sbjct: 421 GFMCKEIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLVAKCRNICAA 470

BLAST of Cp4.1LG12g06410 vs. NCBI nr
Match: XP_022991622.1 (tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita maxima])

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 453/457 (99.12%), Postives = 454/457 (99.34%), Query Frame = 0

Query: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73
           MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHR 133
           CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASG SDSISSEMKRN+VYGMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNVVYGMSGAEFHR 120

Query: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193
           LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253
           LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 313
           ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373
           NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 374 ANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 433
           ANHSEDISD AD KTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVADSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470
           MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Cp4.1LG12g06410 vs. NCBI nr
Match: XP_022954384.1 (tRNA:m(4)X modification enzyme TRM13 homolog [Cucurbita moschata])

HSP 1 Score: 929 bits (2402), Expect = 0.0
Identity = 450/457 (98.47%), Postives = 453/457 (99.12%), Query Frame = 0

Query: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73
           MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHR 133
           CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASG SDSISSEMKRNIVYGMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNIVYGMSGAEFHR 120

Query: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193
           LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253
           LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 313
           ADRSLRQKESLVLERLRIDIEDLNLNAVESLRH+PYLAIGKHLCGPATDLALRCCLTKQS
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373
           NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNK+YLLELGI KEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKKYLLELGIAKEEFLAITWFTSWAVD 360

Query: 374 ANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 433
           ANHSEDISD +D KTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470
           MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Cp4.1LG12g06410 vs. ExPASy TrEMBL
Match: A0A6J1JMC6 (tRNA:m(4)X modification enzyme TRM13 OS=Cucurbita maxima OX=3661 GN=LOC111488184 PE=3 SV=1)

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 453/457 (99.12%), Postives = 454/457 (99.34%), Query Frame = 0

Query: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73
           MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHR 133
           CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASG SDSISSEMKRN+VYGMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNVVYGMSGAEFHR 120

Query: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193
           LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253
           LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 313
           ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373
           NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 374 ANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 433
           ANHSEDISD AD KTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVADSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470
           MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Cp4.1LG12g06410 vs. ExPASy TrEMBL
Match: A0A6J1GSU8 (tRNA:m(4)X modification enzyme TRM13 OS=Cucurbita moschata OX=3662 GN=LOC111456639 PE=3 SV=1)

HSP 1 Score: 929 bits (2402), Expect = 0.0
Identity = 450/457 (98.47%), Postives = 453/457 (99.12%), Query Frame = 0

Query: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73
           MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR
Sbjct: 1   MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 60

Query: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHR 133
           CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASG SDSISSEMKRNIVYGMSGAEFHR
Sbjct: 61  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGLSDSISSEMKRNIVYGMSGAEFHR 120

Query: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193
           LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV
Sbjct: 121 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 180

Query: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253
           LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK
Sbjct: 181 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 240

Query: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 313
           ADRSLRQKESLVLERLRIDIEDLNLNAVESLRH+PYLAIGKHLCGPATDLALRCCLTKQS
Sbjct: 241 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHDPYLAIGKHLCGPATDLALRCCLTKQS 300

Query: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373
           NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNK+YLLELGI KEEFLAITWFTSWAVD
Sbjct: 301 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKKYLLELGIAKEEFLAITWFTSWAVD 360

Query: 374 ANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 433
           ANHSEDISD +D KTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVSDSKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 420

Query: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 470
           MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA
Sbjct: 421 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICAA 457

BLAST of Cp4.1LG12g06410 vs. ExPASy TrEMBL
Match: A0A6J1CSZ2 (tRNA:m(4)X modification enzyme TRM13 OS=Momordica charantia OX=3673 GN=LOC111014027 PE=3 SV=1)

HSP 1 Score: 846 bits (2185), Expect = 2.81e-308
Identity = 411/464 (88.58%), Postives = 431/464 (92.89%), Query Frame = 0

Query: 6   VSEELGLEMASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRE 65
           +SEE  L+MASRCQFWLPKKNRFCAN+PINDSKFCGNHSSRAD QWVPCPVDPSHSVLRE
Sbjct: 1   MSEESALKMASRCQFWLPKKNRFCANAPINDSKFCGNHSSRADGQWVPCPVDPSHSVLRE 60

Query: 66  NLEWHVKRCPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYG 125
           NLEWHVKRCPLLKQ QSLA QPFYQKGINAGEEDSISS+E AS FSDS SSEMKRNIVYG
Sbjct: 61  NLEWHVKRCPLLKQAQSLATQPFYQKGINAGEEDSISSNEQASDFSDSFSSEMKRNIVYG 120

Query: 126 MSGAEFHRLLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASIL 185
           MSGAEFHRLL KIRALHGLICKDIQDS RIKEAC+MWIKGEIDRKIPFQEKHVLQQASIL
Sbjct: 121 MSGAEFHRLLGKIRALHGLICKDIQDSCRIKEACSMWIKGEIDRKIPFQEKHVLQQASIL 180

Query: 186 GNMEEFGVLRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLV 245
           GN+EEFGVLRN DGGEQC+ N S GN+ NV PAV+EFGAGRGYLTQ+LADCYGIKRV LV
Sbjct: 181 GNLEEFGVLRNCDGGEQCDSNGSYGNDTNVPPAVVEFGAGRGYLTQILADCYGIKRVFLV 240

Query: 246 ERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLAL 305
           ERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRH PYLAIGKHLCGPATDL L
Sbjct: 241 ERKSYKLKADRSLRQKESLVLERLRIDIEDLNLNAVESLRHIPYLAIGKHLCGPATDLXL 300

Query: 306 RCCLTKQSNHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAIT 365
           RCCL +QSN+V+TEQ GDRPKL+GLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAIT
Sbjct: 301 RCCLAEQSNNVHTEQHGDRPKLKGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAIT 360

Query: 366 WFTSWAVDANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCK 425
           WFTSWAVDANHSEDISD  + +++LQ SE E  G NGSTVED+ R+MN VERAVLGFMCK
Sbjct: 361 WFTSWAVDANHSEDISDVTNPQSYLQPSEKEDGGTNGSTVEDVVRNMNAVERAVLGFMCK 420

Query: 426 EIIDMGRLMWLKECGLETQLVKYVPSSISPENHLLIAKCRNICA 469
           EIIDMGR MWLKECGLETQLVKYVPSSISPENHLLIAKCRN CA
Sbjct: 421 EIIDMGRQMWLKECGLETQLVKYVPSSISPENHLLIAKCRNNCA 464

BLAST of Cp4.1LG12g06410 vs. ExPASy TrEMBL
Match: A0A1S3CF14 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo OX=3656 GN=LOC103499984 PE=3 SV=1)

HSP 1 Score: 838 bits (2164), Expect = 3.29e-305
Identity = 407/456 (89.25%), Postives = 426/456 (93.42%), Query Frame = 0

Query: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73
           MASRCQFWLPKKNRFCA +PINDSKFCGNHSSRAD   VPCPVDPSHSVLRENL+ HVKR
Sbjct: 1   MASRCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKR 60

Query: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHR 133
           CPLLKQTQSLA QPFYQKGINAGEEDS+S++   SG SDSISSEMKRNIVYGMSG +FHR
Sbjct: 61  CPLLKQTQSLAGQPFYQKGINAGEEDSMSANGPDSGLSDSISSEMKRNIVYGMSGDQFHR 120

Query: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193
           LL KIRALH LICKDIQDSY+IKEACNMWIKGEIDRKIPFQEKHVLQQASILGN++EFGV
Sbjct: 121 LLGKIRALHDLICKDIQDSYQIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGV 180

Query: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253
           LRNYDGG+Q EGNRS GN+ANV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 181 LRNYDGGQQREGNRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240

Query: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 313
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQ 
Sbjct: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQP 300

Query: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373
           N  +TEQC D+ KLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD
Sbjct: 301 NQADTEQCRDKSKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 374 ANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 433
           ANHSEDISD  D KTFLQSSENE DGM G TV++I + MNPVERAVLGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVTDSKTFLQSSENESDGMIGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRL 420

Query: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICA 469
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICA
Sbjct: 421 MWLKECGLETQLVKYVPSTISPENHLLIAKCRNICA 456

BLAST of Cp4.1LG12g06410 vs. ExPASy TrEMBL
Match: A0A5D3CJH7 (tRNA:m(4)X modification enzyme TRM13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00010 PE=3 SV=1)

HSP 1 Score: 835 bits (2158), Expect = 2.70e-304
Identity = 406/456 (89.04%), Postives = 425/456 (93.20%), Query Frame = 0

Query: 14  MASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKR 73
           MASRCQFWLPKKNRFCA +PINDSKFCGNHSSRAD   VPCPVDPSHSVLRENL+ HVKR
Sbjct: 1   MASRCQFWLPKKNRFCAVAPINDSKFCGNHSSRADGDMVPCPVDPSHSVLRENLQGHVKR 60

Query: 74  CPLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHR 133
           CPLLKQTQSLA QPFYQKGINAGEED +S++   SG SDSISSEMKRNIVYGMSG +FHR
Sbjct: 61  CPLLKQTQSLAGQPFYQKGINAGEEDLMSANGPDSGLSDSISSEMKRNIVYGMSGDQFHR 120

Query: 134 LLAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGV 193
           LL KIRALH LICKDIQDSY+IKEACNMWIKGEIDRKIPFQEKHVLQQASILGN++EFGV
Sbjct: 121 LLGKIRALHDLICKDIQDSYQIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNLDEFGV 180

Query: 194 LRNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLK 253
           LRNYDGG+Q EGNRS GN+ANV PAV+EFGAGRGYLTQMLADCYGIKRV LVERKSYKLK
Sbjct: 181 LRNYDGGQQREGNRSYGNDANVVPAVVEFGAGRGYLTQMLADCYGIKRVFLVERKSYKLK 240

Query: 254 ADRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQS 313
           ADRSLRQKESL+LERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQ 
Sbjct: 241 ADRSLRQKESLILERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQP 300

Query: 314 NHVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 373
           N  +TEQC D+ KLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD
Sbjct: 301 NQADTEQCRDKSKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVD 360

Query: 374 ANHSEDISDGADLKTFLQSSENEGDGMNGSTVEDIARHMNPVERAVLGFMCKEIIDMGRL 433
           ANHSEDISD  D KTFLQSSENE DGM G TV++I + MNPVERAVLGFMCKEIIDMGRL
Sbjct: 361 ANHSEDISDVTDSKTFLQSSENESDGMIGCTVDEIVKSMNPVERAVLGFMCKEIIDMGRL 420

Query: 434 MWLKECGLETQLVKYVPSSISPENHLLIAKCRNICA 469
           MWLKECGLETQLVKYVPS+ISPENHLLIAKCRNICA
Sbjct: 421 MWLKECGLETQLVKYVPSTISPENHLLIAKCRNICA 456

BLAST of Cp4.1LG12g06410 vs. TAIR 10
Match: AT4G01880.1 (methyltransferases )

HSP 1 Score: 578.9 bits (1491), Expect = 3.6e-165
Identity = 285/452 (63.05%), Postives = 344/452 (76.11%), Query Frame = 0

Query: 15  ASRCQFWLPKKNRFCANSPINDSKFCGNHSSRADTQWVPCPVDPSHSVLRENLEWHVKRC 74
           ++RC FWLPKK R CAN+ I  S FCGNHS R + QW+PCP+DPSHSVL+ENLEWHVKRC
Sbjct: 6   SNRCSFWLPKKKRSCANTRIESSMFCGNHSQRLEGQWIPCPIDPSHSVLQENLEWHVKRC 65

Query: 75  PLLKQTQSLAVQPFYQKGINAGEEDSISSDELASGFSDSISSEMKRNIVYGMSGAEFHRL 134
           PLLKQT  L+ Q FYQKGINAG ++    +E   G    ++SEMKRN++Y MS ++FH+L
Sbjct: 66  PLLKQTVELSGQRFYQKGINAGNDE----EEEKMGSCSLVTSEMKRNLLYSMSVSKFHQL 125

Query: 135 LAKIRALHGLICKDIQDSYRIKEACNMWIKGEIDRKIPFQEKHVLQQASILGNMEEFGVL 194
           + KI  +HG ICKDI+DSY   EACN+W   EIDRK+PFQEKHVLQQ SILGN+EE GVL
Sbjct: 126 IKKIEDVHGGICKDIEDSYLSPEACNIWFNKEIDRKLPFQEKHVLQQGSILGNLEEIGVL 185

Query: 195 RNYDGGEQCEGNRSCGNEANVAPAVIEFGAGRGYLTQMLADCYGIKRVLLVERKSYKLKA 254
           +  +   +C  +     + N APAV+EFGAGRGYLTQMLADCYGIK+V LVERKSYKLKA
Sbjct: 186 KRCNINVECCESDLEEKDDNTAPAVVEFGAGRGYLTQMLADCYGIKKVYLVERKSYKLKA 245

Query: 255 DRSLRQKESLVLERLRIDIEDLNLNAVESLRHNPYLAIGKHLCGPATDLALRCCLTKQSN 314
           DRSLRQKESLVLER+RIDIEDLNLNAVESL   PY+A+GKHLCGPATDL+LRCCL++Q  
Sbjct: 246 DRSLRQKESLVLERMRIDIEDLNLNAVESLHGVPYVAVGKHLCGPATDLSLRCCLSRQD- 305

Query: 315 HVNTEQCGDRPKLRGLAIATCCHHLCQWKHYTNKRYLLELGITKEEFLAITWFTSWAVDA 374
                  G+ P LRGLAIATCCHHL QWK Y NK Y+L LGI+K+EF  +T FTSWAVD 
Sbjct: 306 -------GESPVLRGLAIATCCHHLSQWKSYINKEYILSLGISKDEFHIMTSFTSWAVDD 365

Query: 375 NHSEDIS--DGADLKTFLQSSENEGDGMNGS--TVEDIARHMNPVERAVLGFMCKEIIDM 434
           +H   +   D  DL       E EG+  + S  +VE++ + M P+ERAVLGF CK+IID 
Sbjct: 366 DHGSKLPGVDDIDLLDSNAKEEEEGEVRDASLISVEEVVKKMKPMERAVLGFKCKQIIDA 425

Query: 435 GRLMWLKECGLETQLVKYVPSSISPENHLLIA 463
           GR+ W+K+ GL ++LVKY+P+SISPEN LLIA
Sbjct: 426 GRMKWVKKHGLNSKLVKYIPASISPENTLLIA 445