Cp4.1LG12g00450 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG12g00450
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	RING-type E3 ubiquitin transferase
Location	Cp4.1LG12: 271851 .. 273378 (-)
RNA-Seq Expression	Cp4.1LG12g00450
Synteny	Cp4.1LG12g00450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGATATCCGCCAAAACCGTCTTCCTCCGTCGCAGCCACTGCCACCGCCACCGGCCGTGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCGACCATGCAAATCATTGTCAACTTTGATATCACTCCAAATTATACACTCAAAAGGCTCATACTCTCTTGGAAAATGAATGAATCTTGCTCCTCCGCCTCCTCCTCGTCGCCCGGACCGCCATCGATGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCGTTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCGGCGGTCGGAGCGAAACCCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAGTCGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCCTGCGAGGAGGCTTTAGGGGTATTGTTAATCTATCGATCGAACGTGGTTAATAAATATAAAAATTTCGGTCGATGAAATACGTTACGATATATTCAATCTTTTATTTATATTCTTAACAGGTATTATCTCAATTTCCATTTTCCAAAACCGAGAAGCTATTTGAGTTACTATCGAGACCAGAAGCGATCAAGTCAATGGCGATCGTTTTACAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAATTACTCCAACAAATATCCAAAAAGGGTTATGATTATAACTCTTTAACGAAACATCATGCCATTGACCTGTTCAAGTCAATATTGGAGCTCGCGTCGGATGAGATAATCAAGAAAGCAAGTACTGTTAAGATGACAGACTCGTCCATAGCTACACAGTGGTATGACATTGTTCAAGCACGAAATTACTTTAGTTGGACGTTTCTAACTAAATCTTTTATTTTTTTTCTAGTCGATACGGAACGTTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTGTTGATTGAAGCTGGTGCTGTTTGCATCATGATCGACCTTCTTCCTGACTCGAACCGATCAAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCATATCATGGGAATAGCGACGGTGACGAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAA

mRNA sequence

ATGGAGGATATCCGCCAAAACCGTCTTCCTCCGTCGCAGCCACTGCCACCGCCACCGGCCGTGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCGACCATGCAAATCATTGTCAACTTTGATATCACTCCAAATTATACACTCAAAAGGCTCATACTCTCTTGGAAAATGAATGAATCTTGCTCCTCCGCCTCCTCCTCGTCGCCCGGACCGCCATCGATGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCGTTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCGGCGGTCGGAGCGAAACCCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAGTCGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCCTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCATTTTCCAAAACCGAGAAGCTATTTGAGTTACTATCGAGACCAGAAGCGATCAAGTCAATGGCGATCGTTTTACAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAATTACTCCAACAAATATCCAAAAAGGGTTATGATTATAACTCTTTAACGAAACATCATGCCATTGACCTGTTCAAGTCAATATTGGAGCTCGCGTCGGATGAGATAATCAAGAAAGCAAGTACTGTTAAGATGACAGACTCGTCCATAGCTACACAGTGTCGATACGGAACGTTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTGTTGATTGAAGCTGGTGCTGTTTGCATCATGATCGACCTTCTTCCTGACTCGAACCGATCAAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCATATCATGGGAATAGCGACGGTGACGAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAA

Coding sequence (CDS)

ATGGAGGATATCCGCCAAAACCGTCTTCCTCCGTCGCAGCCACTGCCACCGCCACCGGCCGTGGAAGATATTCCTCCATACTTCCGGTGCCCTATTTCCATGGAGGTTATGCATGATCCTGTCACGATTTCCACCGGTGTTTCCTTTGAAAGAGCCAATATTGAGAAGTGGTTCTTCACTTACAACAAGAAAACTTGCCCTGCGACCATGCAAATCATTGTCAACTTTGATATCACTCCAAATTATACACTCAAAAGGCTCATACTCTCTTGGAAAATGAATGAATCTTGCTCCTCCGCCTCCTCCTCGTCGCCCGGACCGCCATCGATGGAGTACGATGATGTTGTGGCTCTCTTGAAAAACATCGAGTTGTCGCCGTTCAAAGCGAGTTCTTTGAAGAAACTACGGCTACTCATGGCCATCGACGATGTAGCGGCGGTCGGAGCGAAACCCATCTTTGTTCAAGCAAATGGGTTTGAAGTTCTTGTTGGATTAGTCACCCAAGTCGCCATTTGTGAAAGCTCCAATTTCGCTAATTTTGAAGCCTGCGAGGAGGCTTTAGGGGTATTATCTCAATTTCCATTTTCCAAAACCGAGAAGCTATTTGAGTTACTATCGAGACCAGAAGCGATCAAGTCAATGGCGATCGTTTTACAAAGAGGAAGCATAGAAGGACGATTTTATGCCATGGAATTACTCCAACAAATATCCAAAAAGGGTTATGATTATAACTCTTTAACGAAACATCATGCCATTGACCTGTTCAAGTCAATATTGGAGCTCGCGTCGGATGAGATAATCAAGAAAGCAAGTACTGTTAAGATGACAGACTCGTCCATAGCTACACAGTGTCGATACGGAACGTTATTCTCATCTGCCCTCGAACTTTTGATAGAAATCATGGAATCCTCTAAGAAATGTCGTCTTCTGTTGATTGAAGCTGGTGCTGTTTGCATCATGATCGACCTTCTTCCTGACTCGAACCGATCAAAGTGCGAGAAAATATTACACGTACTCAAACTTCTAAGCGATTGTGCGGAGGGACGGTTAGCCATGGCGGAGCATATCATGGGAATAGCGACGGTGACGAAGAAAATGAGTACATCAAATACAGCTACAAAAATTGGGGTGAAAATCTTATGGTTGATATGCAATTATCATCCTAGTGAGAGGGTTTTAGAGGAGATGATGGGTTGTGGCACAGTGAAGAAGCTGGTAGGGATCTTGAATGGCGATGGAAGATCTTCTTCAACCAAGGAGAAGGCCATGAAGATCATGAAGCTTCATGGGAGTTTTTGGAGGAGATTTCCTTGTTTTCCTTATGAATTTAGGGATTATTTTAAGTTGATAAATTAA

Protein sequence

MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN

Homology

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.0e-45
Identity = 135/426 (31.69%), Postives = 209/426 (49.06%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IPP+F CPIS+E+M DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN+
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNH 70

Query: 83  TLKRLILSW-KMNESCSSASSSSPGPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMAI 142
           TL+RLI SW  +N S       +P PP  + +    +  +      +   LK+LR +++ 
Sbjct: 71  TLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSE 130

Query: 143 DDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKT--E 202
           +       K     A   E L  +V       S++  N    +EAL +L     S+T  +
Sbjct: 131 N----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLK 190

Query: 203 KLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLTKHHAIDLFKSIL 262
            L         +KS+  ++QRG  E R YA  LL+ I +      S+T     ++F  ++
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVV 250

Query: 263 ELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEIMESSKKCRLLLIEAGAVCI 322
           ++  D I +KA+                    +A+ +L+ I    +  R   +EAG + +
Sbjct: 251 QILDDRISQKAT-------------------KAAMHILVNICPWGRN-RHKAVEAGVISV 310

Query: 323 MIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNTATK 382
           +I+LL D    S R   E  + VL LL  CAEGR     H   IA V KK +  S TA+ 
Sbjct: 311 IIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASD 370

Query: 383 IGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWR 441
             V++L  +  +  +  +L EM+  G V KL  +L        TKEKA +++KLH   W+
Sbjct: 371 RAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWK 402

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 4.7e-42
Identity = 132/452 (29.20%), Postives = 221/452 (48.89%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IP +F CPIS+++M DPV +STG++++R +IEKW F+  K +CP T Q+I   D+TPN+
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNH 65

Query: 83  TLKRLILSW-KMNESCSSASSSSPGPPSMEYDDVVALLKNIELSPF-KASSLKKLRLLMA 142
           TL+RLI SW  +N S       +P PP  +  ++  L+K    S   +   LK+LR +++
Sbjct: 66  TLRRLIQSWCTLNASYGIERIPTPKPPICK-SEIEKLIKESSSSHLNQVKCLKRLRQIVS 125

Query: 143 ID-------DVAAVGAKPIFVQANGFEVLVGLVTQVA------ICESSNFAN-FEA---- 202
            +       + A V      + +N  +      + ++      +C+S+   N F++    
Sbjct: 126 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 185

Query: 203 CEEALGVLSQFPFSKT--EKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 262
            +EAL VL     S+T  + L         +K++  ++QRG  E R YA  LL+++ +  
Sbjct: 186 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 245

Query: 263 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 322
                +      +LF  ++++  D+I  KA+   M    +   C +G             
Sbjct: 246 DPMQIILLER--ELFGEVIQILHDQISHKATRSAM--QILVITCPWG------------- 305

Query: 323 MESSKKCRLLLIEAGAVCIMIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHI 382
                + R   +E G + ++I+LL D    S R   E  + VL +L  CAEGR     H 
Sbjct: 306 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 365

Query: 383 MGIATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRS 442
             IA V+KK +  S   ++  V++L  +  +  +  +L+EM+  G V KL  +L      
Sbjct: 366 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCG 425

Query: 443 SSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYF 448
           + TKEKA +++KLH   WR  PC P    D +
Sbjct: 426 NKTKEKAKELLKLHARVWRESPCVPRNLYDSY 433

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 9.5e-35
Identity = 127/438 (29.00%), Postives = 212/438 (48.40%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ-IIVNFDITPN 82
           +IP YF CPIS+E+M DPVT  +G++++R NI KW       +CP T Q + ++ D+TPN
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPN 68

Query: 83  YTLKRLILSWKM-NESCSSASSSSPGPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 142
           + L+RLI  W + NE+      S+P  P  +  +VV  +KN  L  F   +L +   L  
Sbjct: 69  HMLRRLIQHWCVENETRGVVRISTPRVPPGKL-NVVEEIKN--LKKFGQEALGREETLQK 128

Query: 143 IDDVAAVG-AKPIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKTE 202
           ++ +A  G  + +  +    + L+  V +    +       +  +E+L +L        +
Sbjct: 129 LEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSND 188

Query: 203 KLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLTKHHAIDLFKSIL 262
               L+     ++S+  VL +     + Y + LL+ +++  Y  + + +    ++FK I+
Sbjct: 189 AKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKGII 248

Query: 263 ELASD---EIIKKASTVKMTDSSIATQCRYGT-------------LFSSALELLIEIMES 322
               D    + + + TV+ T  S +      T               ++AL +L+E    
Sbjct: 249 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 308

Query: 323 SKKCRLLLIEAGAVCIMIDLLPDSNRSK--CEKILHVLKLLSDCAEGRLAMAEHIMGIAT 382
           S+  R LL++ GAV  +I+L       K   E +L VL  L  CA GR  +  H  GIA 
Sbjct: 309 SRN-RSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAV 368

Query: 383 VTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 439
           VTK+ +  S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D    + KE
Sbjct: 369 VTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD-CGLNLKE 428

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.6e-29
Identity = 123/428 (28.74%), Postives = 198/428 (46.26%), Query Frame = 0


Query: 24  IPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYT 83
           IP +FRCPIS+E+M DPVT+ TG +++RA+IE W    N  TCP T   + +F + PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 84  LKRLILSWKM-NESCSSASSSSPGPPSMEYDDVVALLKNIELSP-------FKASSLKKL 143
           L+RLI  W + N S       +P  P+ +   V ALL               +A++L++L
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPA-DPTSVRALLSQASAITGTHVSVRSRAAALRRL 133

Query: 144 RLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPF 203
           R      D   V    +    N  E+L+ ++        S   + E   E+L +L   P 
Sbjct: 134 RGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLALLVMLPI 193

Query: 204 SKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQIS--KKGYDYNSLTKHHAID 263
           ++  +   + S P  ++ +  +L   SIE R  A  L++ +S   K  D    +  ++  
Sbjct: 194 TEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKG-SISNSES 253

Query: 264 LFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEIMESSKKCRLLLIE 323
           +F+ +L+L  + I           S  A +    TLF+         + S K  R + I 
Sbjct: 254 VFEGVLDLLRNPI----------SSRRALKIGIKTLFA---------LCSVKSTRHIAIT 313

Query: 324 AGAVCIMID-LLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNT 383
           AGA  I+ID L  D +R   E+ L  ++LL    EG  A  EH + +  + K  +  S+ 
Sbjct: 314 AGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR 373

Query: 384 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGS 440
           AT+     L  +C     ER  EE  G G V +L+ ++  +  +   K+KA K++KL   
Sbjct: 374 ATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE-CTERAKKKAQKLLKLLRD 406

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy Swiss-Prot
Match: Q9LXE3 (U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 9.5e-27
Identity = 110/405 (27.16%), Postives = 192/405 (47.41%), Query Frame = 0


Query: 21  VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITP 80
           +  +P +F+CPIS++VM  PV++STGV+++R +I++W    N  TCPATMQI+ N +  P
Sbjct: 8   ITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVP 67

Query: 81  NYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 140
           N TL RLI  W  +     A S SP   +   D++ A ++   +     S  K LR    
Sbjct: 68  NLTLHRLIDHWS-DSINRRADSESPESDTPTRDEINAAIERFRIENDARS--KILRFARE 127

Query: 141 IDDVAA-VGAKPIFVQANGFEVLVGLVTQVAICESSNFANFE--ACEEALGVLSQFPFSK 200
            D+    +  K  FV      +LV L++     +S NF++ +     EA+ +LS      
Sbjct: 128 SDENREFLAGKDDFV-----AMLVDLIS-----DSRNFSDSQLLLVGEAVKILSMI---- 187

Query: 201 TEKLFE--------LLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLTKH 260
             K+F+        L +  + + S  ++++RG+               K   D +++ + 
Sbjct: 188 RRKIFDRRRLSNLILTNGGDCLTSFFLLIKRGN--------------PKLKIDCSAVLEF 247

Query: 261 HAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEIMESSKKCRL 320
            A+D    ++    + ++ +   +  +DS         +L  + L LLI I  SSK+ +L
Sbjct: 248 IAVDAESKLIIAKGEGLVTEIIKLISSDSD-------SSLIEANLSLLIAI-ASSKRVKL 307

Query: 321 LLIEAGAVCIMIDLL--PDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMS 380
            LI    V  +  LL  P ++ S  EK L +L+ +S C EGR  + + +     V K M 
Sbjct: 308 ALIREKLVTKLTSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMK 367

Query: 381 TSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGD 413
            S  AT+  V +LW +C     ++  + ++    V K++ +L  +
Sbjct: 368 VSTAATEHAVTVLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

[Close]

BLAST of Cp4.1LG12g00450 vs. NCBI nr
Match: XP_023549278.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300
           YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Cp4.1LG12g00450 vs. NCBI nr
Match: KAG6574816.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 878 bits (2268), Expect = 0.0
Identity = 443/451 (98.23%), Postives = 447/451 (99.11%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP QPLPPPP VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCSSASSSSP PPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL+RPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Cp4.1LG12g00450 vs. NCBI nr
Match: XP_022958929.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 441/451 (97.78%), Postives = 445/451 (98.67%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP QPLPPPP VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSP PPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDV AVGAKPIFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL+RPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKASTVKMTDSSIAT CRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEM+GCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Cp4.1LG12g00450 vs. NCBI nr
Match: KAG7013396.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 441/451 (97.78%), Postives = 445/451 (98.67%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP QPLPPPP VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLI+SWKMNESCSSASSSSP PPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLIVSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL+RPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKASTVKMTDSSIAT  RYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATH-RYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 450

[Close]

BLAST of Cp4.1LG12g00450 vs. NCBI nr
Match: XP_023006772.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 848 bits (2190), Expect = 2.38e-309
Identity = 431/451 (95.57%), Postives = 437/451 (96.90%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP QP    PA EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQP----PAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCS ASSSSP PPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAV AK IFVQANGFEVLVGLVTQVAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSKTEKLFELLS+ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240

Query: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKAST+K TDSSI TQCRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy TrEMBL
Match: A0A6J1H6I8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075 PE=4 SV=1)

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 441/451 (97.78%), Postives = 445/451 (98.67%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP QPLPPPP VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQPLPPPPVVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSP PPSMEYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPRPPSMEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDV AVGAKPIFVQANGFEVLVGLVTQ+AICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVVAVGAKPIFVQANGFEVLVGLVTQIAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSK EKLFELL+RPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKIEKLFELLTRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240

Query: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKASTVKMTDSSIAT CRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATHCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEM+GCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMIGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy TrEMBL
Match: A0A6J1KWQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 PE=4 SV=1)

HSP 1 Score: 848 bits (2190), Expect = 1.15e-309
Identity = 431/451 (95.57%), Postives = 437/451 (96.90%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDIRQNRLPP QP    PA EDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIRQNRLPPPQP----PAAEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLK 120
           YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCS ASSSSP PPS+EYDDVVALLK
Sbjct: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSFASSSSPRPPSVEYDDVVALLK 120

Query: 121 NIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANF 180
           NIELSPFKASSLKKLRLLMAIDDVAAV AK IFVQANGFEVLVGLVTQVAICESSNFANF
Sbjct: 121 NIELSPFKASSLKKLRLLMAIDDVAAVRAKTIFVQANGFEVLVGLVTQVAICESSNFANF 180

Query: 181 EACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 240
           EACEEALGVLSQFPFSKTEKLFELLS+ EAIKSM IVLQRGSIEGRFYAME+LQQISKKG
Sbjct: 181 EACEEALGVLSQFPFSKTEKLFELLSKLEAIKSMVIVLQRGSIEGRFYAMEILQQISKKG 240

Query: 241 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 300
           YDYNSL+KHHAIDLFKSILELASDEIIKKAST+K TDSSI TQCRYGTLFSSALELLIEI
Sbjct: 241 YDYNSLSKHHAIDLFKSILELASDEIIKKASTIKTTDSSITTQCRYGTLFSSALELLIEI 300

Query: 301 MESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360
           MESSKKCRLLLIEAGA+CIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA
Sbjct: 301 MESSKKCRLLLIEAGAICIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIA 360

Query: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420
           TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE
Sbjct: 361 TVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 420

Query: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN
Sbjct: 421 KAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 447

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy TrEMBL
Match: A0A0A0KBP6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE=4 SV=1)

HSP 1 Score: 754 bits (1947), Expect = 1.40e-272
Identity = 392/454 (86.34%), Postives = 412/454 (90.75%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQPLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFT 60
           MEDI QN  PP  P  P P   D PP+FRCPISME+M DPVTISTGVSFERANIEKWFFT
Sbjct: 1   MEDIHQNPFPP--PHAPTPTTVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFFT 60

Query: 61  YNKKTCPATMQIIVNFDITPNYTLKRLILSWKM-NESCSSASSSSPGPPSMEYDDVVA-L 120
           YNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ++SCSS SS  P   SMEY DVVA L
Sbjct: 61  YNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKDQSCSSTSSLLPSS-SMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFEVL+G+VTQVAICESS+FA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEVLIGIVTQVAICESSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS PEAIKSMAIVLQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIVLQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLI 300
           KGYD+NSLTKH  IDLFKSILELASDEIIKKAS +K TDSSI T CRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSIVTHCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKILH+LKLLS+CAEGR AM EHIMG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILHILKLLSECAEGRSAMGEHIMG 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILN-GDGRSSS 420
           IATVTKKMS SNTATKIG+KILWLICNYHPSERVLEEMMGCGTVKKL+GILN GDGRSS 
Sbjct: 361 IATVTKKMSISNTATKIGMKILWLICNYHPSERVLEEMMGCGTVKKLLGILNNGDGRSS- 420

Query: 421 TKEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           TKEKA K+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 TKEKAKKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy TrEMBL
Match: A0A1S3CC54 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 SV=1)

HSP 1 Score: 747 bits (1929), Expect = 7.72e-270
Identity = 385/453 (84.99%), Postives = 407/453 (89.85%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQ-PLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP   P PPP A  D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVA-L 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCSS S       SMEY DVVA L
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSS--SMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFE+LV +VTQVAICE S+FA
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFA 180

Query: 181 NFEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS PEAIKSMAI+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLI 300
           KGYD+NSLTKH  IDLFKSILELASDEIIKKAS +K  DSS  TQCRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTMDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKIL++LKLLS+CAEGR AM EH MG
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEGRSAMGEHTMG 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420
           IATVTKKMS SNTATKIG+KILW IC+YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 IATVTKKMSISNTATKIGMKILWYICSYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 420

Query: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KEKAMK+MKLHGSFWRRFPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKVMKLHGSFWRRFPCFPYEYRDYLKLIN 450

[Close]

BLAST of Cp4.1LG12g00450 vs. ExPASy TrEMBL
Match: A0A5D3DLY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00740 PE=4 SV=1)

HSP 1 Score: 670 bits (1729), Expect = 5.70e-240
Identity = 354/453 (78.15%), Postives = 376/453 (83.00%), Query Frame = 0


Query: 1   MEDIRQNRLPPSQ-PLPPPPAVEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFF 60
           MEDI Q+  PP   P PPP A  D PP+FRCPISME+M DPVTISTGVSFERANIEKWFF
Sbjct: 1   MEDIHQDPFPPLHGPSPPPTASVDFPPHFRCPISMELMQDPVTISTGVSFERANIEKWFF 60

Query: 61  TYNKKTCPATMQIIVNFDITPNYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVA-L 120
           TYNKKTCPATMQ IVNFDITPNYTLKRLILSWKM ESCSS S       SMEY DVVA L
Sbjct: 61  TYNKKTCPATMQTIVNFDITPNYTLKRLILSWKMKESCSSTSLLPSS--SMEYHDVVADL 120

Query: 121 LKNIELSPFKASSLKKLRLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFA 180
           LKNIE SPFKASSLKKLRLLMA+DDVAAVGAKP+FV+ NGFE+LV +VTQVAICE S+F 
Sbjct: 121 LKNIESSPFKASSLKKLRLLMAVDDVAAVGAKPVFVRVNGFEILVRIVTQVAICECSDFT 180

Query: 181 NFEACEEALGVLSQFPFSKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISK 240
           NFEACEEALGVLSQFPFSKTEK FELLS PEAIKSMAI+LQRGSIEGRFYAME+LQQISK
Sbjct: 181 NFEACEEALGVLSQFPFSKTEKPFELLSEPEAIKSMAIILQRGSIEGRFYAMEMLQQISK 240

Query: 241 KGYDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLI 300
           KGYD+NSLTKH  IDLFKSILELASDEIIKKAS +K TDSS  TQCRYGTLFSSALELLI
Sbjct: 241 KGYDWNSLTKHQIIDLFKSILELASDEIIKKASAIKTTDSSTITQCRYGTLFSSALELLI 300

Query: 301 EIMESSKKCRLLLIEAGAVCIMIDLLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMG 360
           EIMESSKK RLL IEAGAVC+MIDLLPDSNRSKCEKIL++LKLLS+CAEG          
Sbjct: 301 EIMESSKKSRLLSIEAGAVCVMIDLLPDSNRSKCEKILYILKLLSECAEG---------- 360

Query: 361 IATVTKKMSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSST 420
                                     +YHPSERVLEEMMGCGTVKKL+GILNGDG SSST
Sbjct: 361 --------------------------SYHPSERVLEEMMGCGTVKKLLGILNGDG-SSST 414

Query: 421 KEKAMKIMKLHGSFWRRFPCFPYEFRDYFKLIN 451
           KEKAMK+MKLHGSFWR+FPCFPYE+RDY KLIN
Sbjct: 421 KEKAMKVMKLHGSFWRKFPCFPYEYRDYLKLIN 414

[Close]

BLAST of Cp4.1LG12g00450 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-46
Identity = 135/426 (31.69%), Postives = 209/426 (49.06%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IPP+F CPIS+E+M DPV +STG++++R +IEKW F   K +CP T Q I + D+TPN+
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTPNH 70

Query: 83  TLKRLILSW-KMNESCSSASSSSPGPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMAI 142
           TL+RLI SW  +N S       +P PP  + +    +  +      +   LK+LR +++ 
Sbjct: 71  TLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIVSE 130

Query: 143 DDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKT--E 202
           +       K     A   E L  +V       S++  N    +EAL +L     S+T  +
Sbjct: 131 N----ATNKRCLEAAGVPEFLANIV-------SNDSENGSLTDEALNLLYHLETSETVLK 190

Query: 203 KLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLTKHHAIDLFKSIL 262
            L         +KS+  ++QRG  E R YA  LL+ I +      S+T     ++F  ++
Sbjct: 191 NLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP--EVFTEVV 250

Query: 263 ELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEIMESSKKCRLLLIEAGAVCI 322
           ++  D I +KA+                    +A+ +L+ I    +  R   +EAG + +
Sbjct: 251 QILDDRISQKAT-------------------KAAMHILVNICPWGRN-RHKAVEAGVISV 310

Query: 323 MIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNTATK 382
           +I+LL D    S R   E  + VL LL  CAEGR     H   IA V KK +  S TA+ 
Sbjct: 311 IIELLMDESFTSERRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASD 370

Query: 383 IGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGSFWR 441
             V++L  +  +  +  +L EM+  G V KL  +L        TKEKA +++KLH   W+
Sbjct: 371 RAVRVLLSVGRFCATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWK 402

[Close]

BLAST of Cp4.1LG12g00450 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 173.7 bits (439), Expect = 3.3e-43
Identity = 132/452 (29.20%), Postives = 221/452 (48.89%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNY 82
           +IP +F CPIS+++M DPV +STG++++R +IEKW F+  K +CP T Q+I   D+TPN+
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTPNH 65

Query: 83  TLKRLILSW-KMNESCSSASSSSPGPPSMEYDDVVALLKNIELSPF-KASSLKKLRLLMA 142
           TL+RLI SW  +N S       +P PP  +  ++  L+K    S   +   LK+LR +++
Sbjct: 66  TLRRLIQSWCTLNASYGIERIPTPKPPICK-SEIEKLIKESSSSHLNQVKCLKRLRQIVS 125

Query: 143 ID-------DVAAVGAKPIFVQANGFEVLVGLVTQVA------ICESSNFAN-FEA---- 202
            +       + A V      + +N  +      + ++      +C+S+   N F++    
Sbjct: 126 ENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRSL 185

Query: 203 CEEALGVLSQFPFSKT--EKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKG 262
            +EAL VL     S+T  + L         +K++  ++QRG  E R YA  LL+++ +  
Sbjct: 186 MDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEVA 245

Query: 263 YDYNSLTKHHAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEI 322
                +      +LF  ++++  D+I  KA+   M    +   C +G             
Sbjct: 246 DPMQIILLER--ELFGEVIQILHDQISHKATRSAM--QILVITCPWG------------- 305

Query: 323 MESSKKCRLLLIEAGAVCIMIDLLPD----SNRSKCEKILHVLKLLSDCAEGRLAMAEHI 382
                + R   +E G + ++I+LL D    S R   E  + VL +L  CAEGR     H 
Sbjct: 306 -----RNRHKAVEGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHG 365

Query: 383 MGIATVTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRS 442
             IA V+KK +  S   ++  V++L  +  +  +  +L+EM+  G V KL  +L      
Sbjct: 366 AAIAVVSKKILRVSQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCG 425

Query: 443 SSTKEKAMKIMKLHGSFWRRFPCFPYEFRDYF 448
           + TKEKA +++KLH   WR  PC P    D +
Sbjct: 426 NKTKEKAKELLKLHARVWRESPCVPRNLYDSY 433

[Close]

BLAST of Cp4.1LG12g00450 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 149.4 bits (376), Expect = 6.7e-36
Identity = 127/438 (29.00%), Postives = 212/438 (48.40%), Query Frame = 0


Query: 23  DIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQ-IIVNFDITPN 82
           +IP YF CPIS+E+M DPVT  +G++++R NI KW       +CP T Q + ++ D+TPN
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSDLTPN 82

Query: 83  YTLKRLILSWKM-NESCSSASSSSPGPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 142
           + L+RLI  W + NE+      S+P  P  +  +VV  +KN  L  F   +L +   L  
Sbjct: 83  HMLRRLIQHWCVENETRGVVRISTPRVPPGKL-NVVEEIKN--LKKFGQEALGREETLQK 142

Query: 143 IDDVAAVG-AKPIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPFSKTE 202
           ++ +A  G  + +  +    + L+  V +    +       +  +E+L +L        +
Sbjct: 143 LEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSND 202

Query: 203 KLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLTKHHAIDLFKSIL 262
               L+     ++S+  VL +     + Y + LL+ +++  Y  + + +    ++FK I+
Sbjct: 203 AKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTE--YTSSHIVERLNPEIFKGII 262

Query: 263 ELASD---EIIKKASTVKMTDSSIATQCRYGT-------------LFSSALELLIEIMES 322
               D    + + + TV+ T  S +      T               ++AL +L+E    
Sbjct: 263 GFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSW 322

Query: 323 SKKCRLLLIEAGAVCIMIDLLPDSNRSK--CEKILHVLKLLSDCAEGRLAMAEHIMGIAT 382
           S+  R LL++ GAV  +I+L       K   E +L VL  L  CA GR  +  H  GIA 
Sbjct: 323 SRN-RSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAV 382

Query: 383 VTKK-MSTSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKE 439
           VTK+ +  S  A    + IL  +  + P   V+EEM+  GTV+KL  +L  D    + KE
Sbjct: 383 VTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMD-CGLNLKE 442

[Close]

BLAST of Cp4.1LG12g00450 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-30
Identity = 123/428 (28.74%), Postives = 198/428 (46.26%), Query Frame = 0


Query: 24  IPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITPNYT 83
           IP +FRCPIS+E+M DPVT+ TG +++RA+IE W    N  TCP T   + +F + PN+T
Sbjct: 14  IPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPNHT 73

Query: 84  LKRLILSWKM-NESCSSASSSSPGPPSMEYDDVVALLKNIELSP-------FKASSLKKL 143
           L+RLI  W + N S       +P  P+ +   V ALL               +A++L++L
Sbjct: 74  LRRLIQEWCVANRSNGVERIPTPKQPA-DPTSVRALLSQASAITGTHVSVRSRAAALRRL 133

Query: 144 RLLMAIDDVAAVGAKPIFVQANGFEVLVGLVTQVAICESSNFANFEACEEALGVLSQFPF 203
           R      D   V    +    N  E+L+ ++        S   + E   E+L +L   P 
Sbjct: 134 RGFARDSDKNRV----LIAAHNATEILIKIL-------FSETTSSELVSESLALLVMLPI 193

Query: 204 SKTEKLFELLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQIS--KKGYDYNSLTKHHAID 263
           ++  +   + S P  ++ +  +L   SIE R  A  L++ +S   K  D    +  ++  
Sbjct: 194 TEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKG-SISNSES 253

Query: 264 LFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEIMESSKKCRLLLIE 323
           +F+ +L+L  + I           S  A +    TLF+         + S K  R + I 
Sbjct: 254 VFEGVLDLLRNPI----------SSRRALKIGIKTLFA---------LCSVKSTRHIAIT 313

Query: 324 AGAVCIMID-LLPDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKK-MSTSNT 383
           AGA  I+ID L  D +R   E+ L  ++LL    EG  A  EH + +  + K  +  S+ 
Sbjct: 314 AGAPEILIDRLAADFDRCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDR 373

Query: 384 ATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGDGRSSSTKEKAMKIMKLHGS 440
           AT+     L  +C     ER  EE  G G V +L+ ++  +  +   K+KA K++KL   
Sbjct: 374 ATEYAAGALLALCT--AEERWREEAAGAGVVVQLLLMVQSE-CTERAKKKAQKLLKLLRD 406

[Close]

BLAST of Cp4.1LG12g00450 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 6.7e-28
Identity = 110/405 (27.16%), Postives = 192/405 (47.41%), Query Frame = 0


Query: 21  VEDIPPYFRCPISMEVMHDPVTISTGVSFERANIEKWFFTYNKKTCPATMQIIVNFDITP 80
           +  +P +F+CPIS++VM  PV++STGV+++R +I++W    N  TCPATMQI+ N +  P
Sbjct: 8   ITTVPCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQNKEFVP 67

Query: 81  NYTLKRLILSWKMNESCSSASSSSPGPPSMEYDDVVALLKNIELSPFKASSLKKLRLLMA 140
           N TL RLI  W  +     A S SP   +   D++ A ++   +     S  K LR    
Sbjct: 68  NLTLHRLIDHWS-DSINRRADSESPESDTPTRDEINAAIERFRIENDARS--KILRFARE 127

Query: 141 IDDVAA-VGAKPIFVQANGFEVLVGLVTQVAICESSNFANFE--ACEEALGVLSQFPFSK 200
            D+    +  K  FV      +LV L++     +S NF++ +     EA+ +LS      
Sbjct: 128 SDENREFLAGKDDFV-----AMLVDLIS-----DSRNFSDSQLLLVGEAVKILSMI---- 187

Query: 201 TEKLFE--------LLSRPEAIKSMAIVLQRGSIEGRFYAMELLQQISKKGYDYNSLTKH 260
             K+F+        L +  + + S  ++++RG+               K   D +++ + 
Sbjct: 188 RRKIFDRRRLSNLILTNGGDCLTSFFLLIKRGN--------------PKLKIDCSAVLEF 247

Query: 261 HAIDLFKSILELASDEIIKKASTVKMTDSSIATQCRYGTLFSSALELLIEIMESSKKCRL 320
            A+D    ++    + ++ +   +  +DS         +L  + L LLI I  SSK+ +L
Sbjct: 248 IAVDAESKLIIAKGEGLVTEIIKLISSDSD-------SSLIEANLSLLIAI-ASSKRVKL 307

Query: 321 LLIEAGAVCIMIDLL--PDSNRSKCEKILHVLKLLSDCAEGRLAMAEHIMGIATVTKKMS 380
            LI    V  +  LL  P ++ S  EK L +L+ +S C EGR  + + +     V K M 
Sbjct: 308 ALIREKLVTKLTSLLTDPTTSVSVTEKCLKLLEAISSCKEGRSEICDGVCVETVVNKLMK 367

Query: 381 TSNTATKIGVKILWLICNYHPSERVLEEMMGCGTVKKLVGILNGD 413
            S  AT+  V +LW +C     ++  + ++    V K++ +L  +
Sbjct: 368 VSTAATEHAVTVLWSVCYLFKEKKAQDAVIRINGVTKILLLLQSN 372

The following BLAST results are available for this feature:

BLAST of Cp4.1LG12g00450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	2.0e-45	31.69	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	4.7e-42	29.20	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	9.5e-35	29.00	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	1.6e-29	28.74	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9LXE3	9.5e-27	27.16	U-box domain-containing protein 28 OS=Arabidopsis thaliana OX=3702 GN=PUB28 PE=1...	[more]

BLAST of Cp4.1LG12g00450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023549278.1	0.0	100.00	E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo]	[more]
KAG6574816.1	0.0	98.23	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022958929.1	0.0	97.78	E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata]	[more]
KAG7013396.1	0.0	97.78	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023006772.1	2.38e-309	95.57	E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG12g00450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6I8	0.0	97.78	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460075...	[more]
A0A6J1KWQ8	1.15e-309	95.57	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499411 P...	[more]
A0A0A0KBP6	1.40e-272	86.34	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G079190 PE...	[more]
A0A1S3CC54	7.72e-270	84.99	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498994 PE=4 ...	[more]
A0A5D3DLY2	5.70e-240	78.15	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Cp4.1LG12g00450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	1.4e-46	31.69	plant U-box 23	[more]
AT3G52450.1	3.3e-43	29.20	plant U-box 22	[more]
AT3G11840.1	6.7e-36	29.00	plant U-box 24	[more]
AT3G19380.1	1.1e-30	28.74	plant U-box 25	[more]
AT5G09800.1	6.7e-28	27.16	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 27..91 e-value: 6.5E-23 score: 92.1
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 24..95 e-value: 2.4E-16 score: 59.7
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 23..98 score: 29.502176
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 115..429 e-value: 4.3E-12 score: 47.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 18..103 e-value: 2.7E-23 score: 83.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 24..449
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 24..449
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 28..71 e-value: 4.11555E-21 score: 83.8222
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 21..94
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 184..426

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG12g00450.1	Cp4.1LG12g00450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity