Cp4.1LG11g05740 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g05740
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	Cp4.1LG11: 3403146 .. 3404329 (+)
RNA-Seq Expression	Cp4.1LG11g05740
Synteny	Cp4.1LG11g05740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGAGGAAGAGCTTCCTCCCGCGAGCCAATTGATTCCTCTGGACTTCAGCTCAGTTCAAACTATTCCTGAATCTCACTTATGGCCTAACCTTGATGAATCATCGAGGAAGATCGAACCAGACAAAATGGTCTCGATCCCGGTTGTCGATCTTAACAACGATAATGTGTCGGAGCTCATAGGGAAAGCTTGCGAGAAATGGGGAATGTTTCAGTTGATTAATCATGGTATCTCAAAAACCCTAATAGCCCAAACCGAGGAAGTGGCTCGATGTCTTTTCGCTCTTCCAAAAAGCCAGAAGATGAAGACATTACAGGCTCCCGGAAACCCCACCGGCTACAGCATGGCCGGAATATCGAAGTATTATGAGAAACTAATGTGGCATGAAGGATTCACCATCCTAGATTCTCCGGTTGATAGTTTTAAGAAACTCTGGCCATCAAACTACCAACGATTCTGGTAAATCCATGCAACTTTTTTTATGCATGTACACAATTAGGGTCTAGGGTTTAAGGTTTAGGTTAAACCGTTTATTACTCTAAATCCCAACCATTTTTATATTCTCTAATTGGATTCTAACTTTTTTCTTGATCAAGTGATATAATGGAAGAATACCGACTCGAAATGAAGGCTTTAGCTGATAAGCTAATAAGTTTAATCTTCAAGTTTCTTGGCATCTCCGATGAAGAGATGGTGAAGCTATTGCCTTACATCGACCCGAACACCGGAAAACCCCACATGGCTTTGCGCTTGAACTCGTATCCGCCATGCCCTGATCCAAGCAAAGTCATTGGCCTTGCGGCCCACACCGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAAATATGGGCTACAGATCTTCAACGAGAAAGAAGGTTGGATTCCTCTAGCAAGGAAGAGCGACACACTCATTATTAACATCGGTGATTTCCTCCAAATTATATCAAATGGTCGGTTTCATAGCATTCCTCACCGGGTGATGATACAAGAGACTGAGGAGAGTACGATGTCGATGGCATTTTTCTATTATCCACCGAGTCATTTAAACGTATTACCTTATTGTAAGCCATTGAGTGAAACCCCACAGACTCCCATCTACAGAGGAGTGAACGTGAAAGAATATTTCGCCATCAAGGCCAAGCACTCGGGGAAGGGAATTCCTGCCATAACAATATGA

mRNA sequence

ATGTCTGAGGAAGAGCTTCCTCCCGCGAGCCAATTGATTCCTCTGGACTTCAGCTCAGTTCAAACTATTCCTGAATCTCACTTATGGCCTAACCTTGATGAATCATCGAGGAAGATCGAACCAGACAAAATGGTCTCGATCCCGGTTGTCGATCTTAACAACGATAATGTGTCGGAGCTCATAGGGAAAGCTTGCGAGAAATGGGGAATGTTTCAGTTGATTAATCATGGTATCTCAAAAACCCTAATAGCCCAAACCGAGGAAGTGGCTCGATGTCTTTTCGCTCTTCCAAAAAGCCAGAAGATGAAGACATTACAGGCTCCCGGAAACCCCACCGGCTACAGCATGGCCGGAATATCGAAGTATTATGAGAAACTAATGTGGCATGAAGGATTCACCATCCTAGATTCTCCGGTTGATAGTTTTAAGAAACTCTGGCCATCAAACTACCAACGATTCTGTGATATAATGGAAGAATACCGACTCGAAATGAAGGCTTTAGCTGATAAGCTAATAAGTTTAATCTTCAAGTTTCTTGGCATCTCCGATGAAGAGATGGTGAAGCTATTGCCTTACATCGACCCGAACACCGGAAAACCCCACATGGCTTTGCGCTTGAACTCGTATCCGCCATGCCCTGATCCAAGCAAAGTCATTGGCCTTGCGGCCCACACCGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAAATATGGGCTACAGATCTTCAACGAGAAAGAAGGTTGGATTCCTCTAGCAAGGAAGAGCGACACACTCATTATTAACATCGGTGATTTCCTCCAAATTATATCAAATGGTCGGTTTCATAGCATTCCTCACCGGGTGATGATACAAGAGACTGAGGAGAGTACGATGTCGATGGCATTTTTCTATTATCCACCGAGTCATTTAAACGTATTACCTTATTGTAAGCCATTGAGTGAAACCCCACAGACTCCCATCTACAGAGGAGTGAACGTGAAAGAATATTTCGCCATCAAGGCCAAGCACTCGGGGAAGGGAATTCCTGCCATAACAATATGA

Coding sequence (CDS)

ATGTCTGAGGAAGAGCTTCCTCCCGCGAGCCAATTGATTCCTCTGGACTTCAGCTCAGTTCAAACTATTCCTGAATCTCACTTATGGCCTAACCTTGATGAATCATCGAGGAAGATCGAACCAGACAAAATGGTCTCGATCCCGGTTGTCGATCTTAACAACGATAATGTGTCGGAGCTCATAGGGAAAGCTTGCGAGAAATGGGGAATGTTTCAGTTGATTAATCATGGTATCTCAAAAACCCTAATAGCCCAAACCGAGGAAGTGGCTCGATGTCTTTTCGCTCTTCCAAAAAGCCAGAAGATGAAGACATTACAGGCTCCCGGAAACCCCACCGGCTACAGCATGGCCGGAATATCGAAGTATTATGAGAAACTAATGTGGCATGAAGGATTCACCATCCTAGATTCTCCGGTTGATAGTTTTAAGAAACTCTGGCCATCAAACTACCAACGATTCTGTGATATAATGGAAGAATACCGACTCGAAATGAAGGCTTTAGCTGATAAGCTAATAAGTTTAATCTTCAAGTTTCTTGGCATCTCCGATGAAGAGATGGTGAAGCTATTGCCTTACATCGACCCGAACACCGGAAAACCCCACATGGCTTTGCGCTTGAACTCGTATCCGCCATGCCCTGATCCAAGCAAAGTCATTGGCCTTGCGGCCCACACCGACACCTCCCTCTTCACCATGCTTCACCAGGCACGCAAATATGGGCTACAGATCTTCAACGAGAAAGAAGGTTGGATTCCTCTAGCAAGGAAGAGCGACACACTCATTATTAACATCGGTGATTTCCTCCAAATTATATCAAATGGTCGGTTTCATAGCATTCCTCACCGGGTGATGATACAAGAGACTGAGGAGAGTACGATGTCGATGGCATTTTTCTATTATCCACCGAGTCATTTAAACGTATTACCTTATTGTAAGCCATTGAGTGAAACCCCACAGACTCCCATCTACAGAGGAGTGAACGTGAAAGAATATTTCGCCATCAAGGCCAAGCACTCGGGGAAGGGAATTCCTGCCATAACAATATGA

Protein sequence

MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSELIGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI

Homology

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-76
Identity = 139/337 (41.25%), Postives = 210/337 (62.31%), Query Frame = 0


Query: 16  DFSSVQTIPESHLWPNLDE----SSRKIEPDKMVSIPVVDLNNDNVSELIGKACEKWGMF 75
           DF+S+Q +PES+ W +LD+     S  I  +   ++PV+DLN+ N S+LIG AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWHEG 135
           Q++NHGI  +L+   + + + LF+LP  QK K  ++P   +GY +A IS ++ KLMW+EG
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYEG 140

Query: 136 FTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVKLLP 195
           FTI+ SP+D F++LWP +Y RFCDI+ +Y   MK LA  L+ L+   LGI+ E++     
Sbjct: 141 FTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDI--KWA 200

Query: 196 YIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEKEGWI 255
                  K   AL+LNSYP CPDP   +GLA HTD++  T+L Q    GLQ+  E  GWI
Sbjct: 201 GSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWI 260

Query: 256 PLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSHLNVLPYC 315
            +      L++N+GD   I+SNG + S+ HRV++  T +   S+A+ Y PPS++ + P+ 
Sbjct: 261 TVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQ-RFSVAYLYGPPSNVEICPHA 320

Query: 316 KPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 349
           K +  T + P+YR V   EY   KAKH  K + ++ +
Sbjct: 321 KLIGPT-KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 261.9 bits (668), Expect = 1.0e-68
Identity = 128/334 (38.32%), Postives = 202/334 (60.48%), Query Frame = 0


Query: 16  DFSSVQTIPESHLWPNLDE---SSRKIEPDKMVSIPVVDLNNDNVSELIGKACEKWGMFQ 75
           DF+S++ +P+S+ W   D+   S+    P    +IP++DL++ + +  IG AC  WG FQ
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHACRTWGAFQ 82

Query: 76  LINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWHEGF 135
           + NHG+   L+   E +   LF LP  +K+K+ ++    +GY +A I+ ++ K MW EGF
Sbjct: 83  ISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEGF 142

Query: 136 TILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVKLLPY 195
           TI  SP++ F+KLWP ++  +CDI+EEY   MK LA KL+ L    LG+S+E++      
Sbjct: 143 TITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDIEWASLS 202

Query: 196 IDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEKEGWIP 255
            D N  +   AL+LN YP CP+P + +GLAAHTD++L T+L+Q    GLQ+F +  GW+ 
Sbjct: 203 SDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 256 LARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSHLNVLPYCK 315
           +     +L++N+GD   I+SNG F S+ HR  + +T  + +S+AF + P S + + P  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK 322

Query: 316 PLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAI 347
            +S   ++P+Y+ V  KEY   KA H  K +  I
Sbjct: 323 LVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMI 352

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 7.5e-64
Identity = 120/335 (35.82%), Postives = 196/335 (58.51%), Query Frame = 0


Query: 14  PLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSELIGKACEKWGMFQL 73
           P DF S   +P+S+ W   D+           ++P++DL++ +V+ L+G AC  WG FQ+
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASD-ETLPLIDLSDIHVATLVGHACTTWGAFQI 80

Query: 74  INHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWHEGFT 133
            NHG+   L+   E +   LF LP  +K+K  ++    +GY +A I+ ++ K MW EGFT
Sbjct: 81  TNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGFT 140

Query: 134 ILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVKLLPYI 193
           ++ SP+  F+KLWPS++ ++C+I+EEY   M+ LA KL+      LG+ +++    + + 
Sbjct: 141 VIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKD----IQWA 200

Query: 194 DPNTG--KPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEKEGWI 253
            PN+        ++LN YP CP+P + +GLAAHTD++L T+L+Q    GLQ+F +  GW+
Sbjct: 201 GPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWV 260

Query: 254 PLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSHLNVLPYC 313
                  +L++N+GD L I++NG F S+ HR  +     S  SMA+ + PPS + + P  
Sbjct: 261 TAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV-RSRFSMAYLWGPPSDIMISPLP 320

Query: 314 KPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAI 347
           K L +  Q+P+Y  +  K+Y A KA H  + +  I
Sbjct: 321 K-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSII 345

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy Swiss-Prot
Match: Q3I409 (Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum OX=4565 GN=GA3ox2-3 PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.7e-63
Identity = 126/345 (36.52%), Postives = 193/345 (55.94%), Query Frame = 0


Query: 16  DFSSVQTIPESHLWPNL------DESSRKIEPDKMVSIPVVDLNNDNVSELIGKACEKWG 75
           DF + + +PE+H WP L      D S    EPD   ++PVVD+ +   +E +G A + WG
Sbjct: 15  DFRAARRVPETHAWPGLHDHPVVDGSGAGGEPD---AVPVVDMRDPFAAEAVGLAAQDWG 74

Query: 76  MFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWH 135
            F L+ HG+   L+ + E     +FALP S+KM+ ++ PG+  GY    IS ++ K MW 
Sbjct: 75  AFLLVGHGVPLDLLVRVEAAIAGMFALPASEKMRAVRRPGDSCGYGSPPISSFFSKCMWS 134

Query: 136 EGFTILDSPVDS-FKKLWPS---NYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEE 195
           EG+T   + + S  +KLWP    +Y+ FC +MEE+  EM+ALADKL+ L    LG++ E+
Sbjct: 135 EGYTFSPANLRSDLRKLWPKAGHDYRHFCAVMEEFHREMRALADKLLELFLVALGLTGEQ 194

Query: 196 MVKL---LPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQ 255
           +  +       +  T   H    LN YP CPDP + +GL AHTD+  FT + Q+   GLQ
Sbjct: 195 VAAVESEQKIAETMTATMH----LNWYPKCPDPKRALGLIAHTDSGFFTFVLQSLVPGLQ 254

Query: 256 IFNE-KEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYP 315
           +F    + W+ +      +++N+GD  QI++NGRFHS+ HR ++   E   +S+ +F  P
Sbjct: 255 LFRHGPDRWVTVPAVPGAMVVNVGDLFQILTNGRFHSVYHRAVVNR-ESDRISLGYFLGP 314

Query: 316 PSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAI 347
           P+H+ V P  + L+ TP    YR V   EY  ++ K    G  A+
Sbjct: 315 PAHVKVAPLREALAGTPAA--YRAVTWPEYMGVRKKAFTTGASAL 349

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.4e-62
Identity = 128/339 (37.76%), Postives = 202/339 (59.59%), Query Frame = 0


Query: 11  QLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVS-IPVVDLNNDNVSELIGKACEKWG 70
           ++IPLDF++ +T+P+SH+W        K EP+     IPV+ L+N     L+ +ACE+WG
Sbjct: 17  RIIPLDFTNTKTLPDSHVW-------SKPEPETTSGPIPVISLSNPEEHGLLRQACEEWG 76

Query: 71  MFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWH 130
           +F + +HG+S +L+   +   + LF+LP  +K+  +++P   TGY +  IS +Y+KLMW 
Sbjct: 77  VFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWS 136

Query: 131 EGFTILDSPVDSFKK-LWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVK 190
           EGF+++ S +      LWP ++  FC++MEEY+  M  L+ +LIS++   LG++ E++  
Sbjct: 137 EGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGW 196

Query: 191 LLP-YIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEK 250
           L+P      T      L+LNSYP CPDP   +GLA HTD+SL T+L+Q    GL+I + +
Sbjct: 197 LVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQ 256

Query: 251 E---GWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSH 310
           E    WI +     +L++ +GD   IISNG+F S  HR ++ +T    +S A+F  PP +
Sbjct: 257 EEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPPKN 316

Query: 311 LNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGI 344
           L + P     S+    PIYR +  +EY A KA H  K +
Sbjct: 317 LQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

[Close]

BLAST of Cp4.1LG11g05740 vs. NCBI nr
Match: XP_023545777.1 (gibberellin 3-beta-dioxygenase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 709 bits (1831), Expect = 4.62e-258
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL
Sbjct: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS
Sbjct: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG
Sbjct: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG
Sbjct: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI
Sbjct: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. NCBI nr
Match: KAG6598831.1 (Gibberellin 3-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 675 bits (1742), Expect = 1.71e-244
Identity = 329/348 (94.54%), Postives = 341/348 (97.99%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           MS+EELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKM+SIPVVDLN+DNVSEL
Sbjct: 1   MSDEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMISIPVVDLNDDNVSEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACEKWGMFQLINHGISKTLIA+TEEVARCLFALPKSQKMKTLQ+P NPTGYSMAGIS
Sbjct: 61  IGKACEKWGMFQLINHGISKTLIAETEEVARCLFALPKSQKMKTLQSPENPTGYSMAGIS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFTILDSPVD FKKLWPS+YQRFCDIMEEYRL+MKALADKLI LIFKFLG
Sbjct: 121 KYYEKLMWHEGFTILDSPVDGFKKLWPSDYQRFCDIMEEYRLKMKALADKLIRLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEE+VKLLPYIDP TGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG
Sbjct: 181 ISDEEIVKLLPYIDPTTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLAR+SDTLIINIGDFLQIISNGRFHSIPHRVMIQET+ESTMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARRSDTLIINIGDFLQIISNGRFHSIPHRVMIQETKESTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PPSHLNV PYCKPLSETPQTPIY+GVNVKEYFAIKAK+SG GI AITI
Sbjct: 301 PPSHLNVSPYCKPLSETPQTPIYKGVNVKEYFAIKAKNSGNGILAITI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. NCBI nr
Match: XP_022997424.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 675 bits (1741), Expect = 2.42e-244
Identity = 329/348 (94.54%), Postives = 339/348 (97.41%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           MS +ELPPASQLIPLDFSSVQTIPESHLWPNLDE SRKIEPDKM+SIPVVDLN+DNVSEL
Sbjct: 1   MSNQELPPASQLIPLDFSSVQTIPESHLWPNLDELSRKIEPDKMLSIPVVDLNDDNVSEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACE WGMFQLINHGISKTLIA+TEEVARCLFALPKSQKMKTLQAPG PTGY MAGIS
Sbjct: 61  IGKACENWGMFQLINHGISKTLIAETEEVARCLFALPKSQKMKTLQAPGTPTGYCMAGIS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFTILDSPVD FKKLWPS+YQRFCDIMEEYRL+MKALADKLISLIFKFLG
Sbjct: 121 KYYEKLMWHEGFTILDSPVDGFKKLWPSDYQRFCDIMEEYRLKMKALADKLISLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEEMVKLLPYIDP TGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG
Sbjct: 181 ISDEEMVKLLPYIDPITGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLAR+SDTLIINIGDFLQIISNGRFHSIPHRVMIQET+ESTMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARRSDTLIINIGDFLQIISNGRFHSIPHRVMIQETDESTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PPSHLNV PYCKPLSETPQTPIY+GVNVKEYFAIKAK+SG GIPAITI
Sbjct: 301 PPSHLNVSPYCKPLSETPQTPIYKGVNVKEYFAIKAKNSGNGIPAITI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. NCBI nr
Match: XP_022929631.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 665 bits (1717), Expect = 1.10e-240
Identity = 324/348 (93.10%), Postives = 337/348 (96.84%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           MS+E+LPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKM+SIPVVDLN+DNVSEL
Sbjct: 1   MSDEDLPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMISIPVVDLNDDNVSEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACEKWGMFQLINHGISKTLI +TEEVARCLFALPKSQKMKTLQ+P  PTGYSMAGIS
Sbjct: 61  IGKACEKWGMFQLINHGISKTLITETEEVARCLFALPKSQKMKTLQSPETPTGYSMAGIS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFTILDSPVD FKKLWPS+YQRFCDIMEEYRL+MKALADKLI LIFKFLG
Sbjct: 121 KYYEKLMWHEGFTILDSPVDGFKKLWPSDYQRFCDIMEEYRLKMKALADKLIRLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEE+VKLL YIDP TGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG
Sbjct: 181 ISDEEIVKLLSYIDPTTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLAR+SDTLIINIGDFLQIISNGRFHSIPHRVMIQET+ESTMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARRSDTLIINIGDFLQIISNGRFHSIPHRVMIQETKESTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PPSHLNV PYCKPLSETPQTPIY+GVNVKEYF IKAK+SG GI AITI
Sbjct: 301 PPSHLNVSPYCKPLSETPQTPIYKGVNVKEYFTIKAKNSGNGILAITI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. NCBI nr
Match: XP_023545778.1 (gibberellin 3-beta-dioxygenase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 660 bits (1703), Expect = 1.50e-238
Identity = 321/348 (92.24%), Postives = 337/348 (96.84%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL
Sbjct: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS
Sbjct: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG
Sbjct: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEEMVKLLPYIDPNTGKPHMALRLNS+P CP+PSKVIGLAAHTDTSLFTMLHQAR+ G
Sbjct: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSFPACPEPSKVIGLAAHTDTSLFTMLHQARREG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQI NEK+GW+PLA +SD LIIN+GDFLQIISNGRFHS+PHRVMI+ETE++TMSMA+F++
Sbjct: 241 LQILNEKDGWLPLAPRSDALIINVGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFH 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PP HL+V PYCKPLSET QTPIY+GVNVKEYF IKAK SGKGI AITI
Sbjct: 301 PPGHLHVAPYCKPLSETLQTPIYKGVNVKEYFVIKAKASGKGIAAITI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy TrEMBL
Match: A0A6J1KBB1 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492344 PE=3 SV=1)

HSP 1 Score: 675 bits (1741), Expect = 1.17e-244
Identity = 329/348 (94.54%), Postives = 339/348 (97.41%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           MS +ELPPASQLIPLDFSSVQTIPESHLWPNLDE SRKIEPDKM+SIPVVDLN+DNVSEL
Sbjct: 1   MSNQELPPASQLIPLDFSSVQTIPESHLWPNLDELSRKIEPDKMLSIPVVDLNDDNVSEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACE WGMFQLINHGISKTLIA+TEEVARCLFALPKSQKMKTLQAPG PTGY MAGIS
Sbjct: 61  IGKACENWGMFQLINHGISKTLIAETEEVARCLFALPKSQKMKTLQAPGTPTGYCMAGIS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFTILDSPVD FKKLWPS+YQRFCDIMEEYRL+MKALADKLISLIFKFLG
Sbjct: 121 KYYEKLMWHEGFTILDSPVDGFKKLWPSDYQRFCDIMEEYRLKMKALADKLISLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEEMVKLLPYIDP TGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG
Sbjct: 181 ISDEEMVKLLPYIDPITGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLAR+SDTLIINIGDFLQIISNGRFHSIPHRVMIQET+ESTMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARRSDTLIINIGDFLQIISNGRFHSIPHRVMIQETDESTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PPSHLNV PYCKPLSETPQTPIY+GVNVKEYFAIKAK+SG GIPAITI
Sbjct: 301 PPSHLNVSPYCKPLSETPQTPIYKGVNVKEYFAIKAKNSGNGIPAITI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy TrEMBL
Match: A0A6J1ENP0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436158 PE=3 SV=1)

HSP 1 Score: 665 bits (1717), Expect = 5.34e-241
Identity = 324/348 (93.10%), Postives = 337/348 (96.84%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           MS+E+LPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKM+SIPVVDLN+DNVSEL
Sbjct: 1   MSDEDLPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMISIPVVDLNDDNVSEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACEKWGMFQLINHGISKTLI +TEEVARCLFALPKSQKMKTLQ+P  PTGYSMAGIS
Sbjct: 61  IGKACEKWGMFQLINHGISKTLITETEEVARCLFALPKSQKMKTLQSPETPTGYSMAGIS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFTILDSPVD FKKLWPS+YQRFCDIMEEYRL+MKALADKLI LIFKFLG
Sbjct: 121 KYYEKLMWHEGFTILDSPVDGFKKLWPSDYQRFCDIMEEYRLKMKALADKLIRLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEE+VKLL YIDP TGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG
Sbjct: 181 ISDEEIVKLLSYIDPTTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLAR+SDTLIINIGDFLQIISNGRFHSIPHRVMIQET+ESTMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARRSDTLIINIGDFLQIISNGRFHSIPHRVMIQETKESTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PPSHLNV PYCKPLSETPQTPIY+GVNVKEYF IKAK+SG GI AITI
Sbjct: 301 PPSHLNVSPYCKPLSETPQTPIYKGVNVKEYFTIKAKNSGNGILAITI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy TrEMBL
Match: A0A6J1K7G4 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492350 PE=3 SV=1)

HSP 1 Score: 602 bits (1553), Expect = 5.23e-216
Identity = 290/348 (83.33%), Postives = 314/348 (90.23%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           M++++LPPAS ++PLDF SVQT+PESHLW + DESS+KIE DK+VSIPVVDLN+DNV EL
Sbjct: 1   MTDQKLPPASLVVPLDFDSVQTVPESHLWAHTDESSQKIESDKIVSIPVVDLNDDNVMEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKACE+WGMFQLINHGI KTLIA+TEEVA+CLFALPKSQKMK LQ PG  TGY +A  S
Sbjct: 61  IGKACEEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGFT++ SPVD FKKLWPS+YQRFCD MEEY+L+MKAL DKL  LIFKFLG
Sbjct: 121 KYYEKLMWHEGFTVIGSPVDDFKKLWPSDYQRFCDKMEEYQLKMKALGDKLTRLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           ISDEEMVKLL YIDP T KPHM LRLNSYPPCPDPSKVIGLA HTDTSL TMLHQARKYG
Sbjct: 181 ISDEEMVKLLSYIDPTTRKPHMLLRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLARKSDTLIINIGD LQIISNGRFHSIPHRVMIQETEE+TMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARKSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PP HLNV PYCKPLSETPQTPIY+ VNVKEYF IKA + GKGI A+TI
Sbjct: 301 PPGHLNVSPYCKPLSETPQTPIYKAVNVKEYFTIKANNLGKGIAALTI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy TrEMBL
Match: A0A6J1ENA0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436159 PE=3 SV=1)

HSP 1 Score: 598 bits (1542), Expect = 2.48e-214
Identity = 288/348 (82.76%), Postives = 316/348 (90.80%), Query Frame = 0


Query: 1   MSEEELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSEL 60
           M++++LPPAS ++PLDF SVQTIPESHLWP+ DESS+KIE DK+VS+PVVDLN+DNV EL
Sbjct: 1   MADQKLPPASLVVPLDFDSVQTIPESHLWPHPDESSQKIESDKIVSVPVVDLNDDNVLEL 60

Query: 61  IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGIS 120
           IGKAC++WGMFQLINHGI KTLIA+TEEVA+CLFALPKSQKMK LQ PG  TGY +A  S
Sbjct: 61  IGKACKEWGMFQLINHGIPKTLIAETEEVAQCLFALPKSQKMKALQVPGTSTGYCIARFS 120

Query: 121 KYYEKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLG 180
           KYYEKLMWHEGF+I+ SPVD FKKLWPS+YQRFCD MEEY+L+MKAL DKLI LIFKFLG
Sbjct: 121 KYYEKLMWHEGFSIIGSPVDDFKKLWPSDYQRFCDKMEEYQLKMKALGDKLIRLIFKFLG 180

Query: 181 ISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYG 240
           I DEEMVKLL YIDP T KPHM  RLNSYPPCPDPSKVIGLA HTDTSL TMLHQARKYG
Sbjct: 181 IFDEEMVKLLSYIDPTTEKPHMLFRLNSYPPCPDPSKVIGLATHTDTSLLTMLHQARKYG 240

Query: 241 LQIFNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYY 300
           LQIFNEKEGWIPLAR+SDTLIINIGD LQIISNGRFHSIPHRVMIQETEE+TMSMAFFYY
Sbjct: 241 LQIFNEKEGWIPLARRSDTLIINIGDLLQIISNGRFHSIPHRVMIQETEETTMSMAFFYY 300

Query: 301 PPSHLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           PPS+LNV PYCKPLSETP TPIY+GVNVKEYFAIKA + G+GI A+TI
Sbjct: 301 PPSYLNVSPYCKPLSETPHTPIYKGVNVKEYFAIKANNLGEGIAALTI 348

[Close]

BLAST of Cp4.1LG11g05740 vs. ExPASy TrEMBL
Match: A0A6J1ESR0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436163 PE=3 SV=1)

HSP 1 Score: 563 bits (1450), Expect = 2.37e-200
Identity = 267/345 (77.39%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 4   EELPPASQLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSELIGK 63
           ++LP    L PLD S+V  +PESH+WP+LDESS+K E DK VSIPVVD N+DNV ELIGK
Sbjct: 2   DKLPAVGLLTPLDLSTVDAVPESHVWPHLDESSQKFESDKTVSIPVVDFNDDNVLELIGK 61

Query: 64  ACEKWGMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYY 123
           ACE+WGMFQLINHGI KTL A+TEEVARCLFALP+SQKMK L  PG   GY MA ++K++
Sbjct: 62  ACEEWGMFQLINHGIPKTLTAETEEVARCLFALPQSQKMKALNVPGTANGYCMARLTKHH 121

Query: 124 EKLMWHEGFTILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISD 183
           +K+MWHEGFT++ SPVD FKKLWPSNYQRFCD MEEY+L+MKALADKLISLIFKFLGISD
Sbjct: 122 DKMMWHEGFTVIGSPVDDFKKLWPSNYQRFCDKMEEYQLKMKALADKLISLIFKFLGISD 181

Query: 184 EEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQI 243
           EEMVK L YIDP TGKPH+ALRLNS+PPCP+PSKVIGLAAHTDTSLFTMLHQAR+ GLQI
Sbjct: 182 EEMVKKLSYIDPATGKPHLALRLNSFPPCPEPSKVIGLAAHTDTSLFTMLHQARREGLQI 241

Query: 244 FNEKEGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPS 303
            NEK+GW+PLA +SD LIIN+GDFLQIISNGRFHS+PHRVMI+ETE++TMSMA+F++PP 
Sbjct: 242 LNEKDGWLPLAPRSDALIINVGDFLQIISNGRFHSVPHRVMIRETEKTTMSMAYFFHPPG 301

Query: 304 HLNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 348
           HL V PYCKPLSET QTPIY+GVNVKEYF IKAK SGKGI A+TI
Sbjct: 302 HLYVAPYCKPLSETLQTPIYKGVNVKEYFIIKAKASGKGIAALTI 346

[Close]

BLAST of Cp4.1LG11g05740 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 261.9 bits (668), Expect = 7.2e-70
Identity = 128/334 (38.32%), Postives = 202/334 (60.48%), Query Frame = 0


Query: 16  DFSSVQTIPESHLWPNLDE---SSRKIEPDKMVSIPVVDLNNDNVSELIGKACEKWGMFQ 75
           DF+S++ +P+S+ W   D+   S+    P    +IP++DL++ + +  IG AC  WG FQ
Sbjct: 23  DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHACRTWGAFQ 82

Query: 76  LINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWHEGF 135
           + NHG+   L+   E +   LF LP  +K+K+ ++    +GY +A I+ ++ K MW EGF
Sbjct: 83  ISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFNKQMWSEGF 142

Query: 136 TILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVKLLPY 195
           TI  SP++ F+KLWP ++  +CDI+EEY   MK LA KL+ L    LG+S+E++      
Sbjct: 143 TITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEEDIEWASLS 202

Query: 196 IDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEKEGWIP 255
            D N  +   AL+LN YP CP+P + +GLAAHTD++L T+L+Q    GLQ+F +  GW+ 
Sbjct: 203 SDLNWAQ--AALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVT 262

Query: 256 LARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSHLNVLPYCK 315
           +     +L++N+GD   I+SNG F S+ HR  + +T  + +S+AF + P S + + P  K
Sbjct: 263 VPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQT-RARLSVAFLWGPQSDIKISPVPK 322

Query: 316 PLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAI 347
            +S   ++P+Y+ V  KEY   KA H  K +  I
Sbjct: 323 LVSPV-ESPLYQSVTWKEYLRTKATHFNKALSMI 352

[Close]

BLAST of Cp4.1LG11g05740 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 245.7 bits (626), Expect = 5.3e-65
Identity = 120/335 (35.82%), Postives = 196/335 (58.51%), Query Frame = 0


Query: 14  PLDFSSVQTIPESHLWPNLDESSRKIEPDKMVSIPVVDLNNDNVSELIGKACEKWGMFQL 73
           P DF S   +P+S+ W   D+           ++P++DL++ +V+ L+G AC  WG FQ+
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASD-ETLPLIDLSDIHVATLVGHACTTWGAFQI 80

Query: 74  INHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWHEGFT 133
            NHG+   L+   E +   LF LP  +K+K  ++    +GY +A I+ ++ K MW EGFT
Sbjct: 81  TNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKMWSEGFT 140

Query: 134 ILDSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVKLLPYI 193
           ++ SP+  F+KLWPS++ ++C+I+EEY   M+ LA KL+      LG+ +++    + + 
Sbjct: 141 VIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKD----IQWA 200

Query: 194 DPNTG--KPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEKEGWI 253
            PN+        ++LN YP CP+P + +GLAAHTD++L T+L+Q    GLQ+F +  GW+
Sbjct: 201 GPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWV 260

Query: 254 PLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSHLNVLPYC 313
                  +L++N+GD L I++NG F S+ HR  +     S  SMA+ + PPS + + P  
Sbjct: 261 TAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHV-RSRFSMAYLWGPPSDIMISPLP 320

Query: 314 KPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAI 347
           K L +  Q+P+Y  +  K+Y A KA H  + +  I
Sbjct: 321 K-LVDPLQSPLYPSLTWKQYLATKATHFNQSLSII 345

[Close]

BLAST of Cp4.1LG11g05740 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-63
Identity = 126/342 (36.84%), Postives = 197/342 (57.60%), Query Frame = 0


Query: 16  DFSSVQTIPESHLWPNLDESSRKIEPDKMV-------SIPVVDLNNDNVSELIGKACEKW 75
           +  S+QT+PES  W  L  +   + P   V       SIPV+DL+N +V+ LIG A + W
Sbjct: 12  NLGSLQTLPESFTW-KLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTW 71

Query: 76  GMFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMW 135
           G FQ+ NHGIS+ L+   E +++ LF +P  +K++   +    +GY    IS ++EK MW
Sbjct: 72  GAFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMW 131

Query: 136 HEGFTIL-DSPVDSFKKLWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMV 195
            EGFTI  DS  + F  LWP ++ ++C I++EY  EM+ LA +L+  I   LG++ E++ 
Sbjct: 132 SEGFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIE 191

Query: 196 KLLPYIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEK 255
                    +     A+RLN YP CP+P + +GLAAHTD+++ T+LHQ+   GLQ+F E+
Sbjct: 192 WAHKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREE 251

Query: 256 EGWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFY-YPPSHLN 315
            GW+ +      L++NIGD   I+SNG+  S+ HR  +  T  S +S+A+ +  P   + 
Sbjct: 252 SGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHT-RSRISIAYLWGGPAGDVQ 311

Query: 316 VLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGIPAITI 349
           + P  K L+   +  +YR +  KEY  IK +   K + AI +
Sbjct: 312 IAPISK-LTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of Cp4.1LG11g05740 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-63
Identity = 128/339 (37.76%), Postives = 202/339 (59.59%), Query Frame = 0


Query: 11  QLIPLDFSSVQTIPESHLWPNLDESSRKIEPDKMVS-IPVVDLNNDNVSELIGKACEKWG 70
           ++IPLDF++ +T+P+SH+W        K EP+     IPV+ L+N     L+ +ACE+WG
Sbjct: 17  RIIPLDFTNTKTLPDSHVW-------SKPEPETTSGPIPVISLSNPEEHGLLRQACEEWG 76

Query: 71  MFQLINHGISKTLIAQTEEVARCLFALPKSQKMKTLQAPGNPTGYSMAGISKYYEKLMWH 130
           +F + +HG+S +L+   +   + LF+LP  +K+  +++P   TGY +  IS +Y+KLMW 
Sbjct: 77  VFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWS 136

Query: 131 EGFTILDSPVDSFKK-LWPSNYQRFCDIMEEYRLEMKALADKLISLIFKFLGISDEEMVK 190
           EGF+++ S +      LWP ++  FC++MEEY+  M  L+ +LIS++   LG++ E++  
Sbjct: 137 EGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGW 196

Query: 191 LLP-YIDPNTGKPHMALRLNSYPPCPDPSKVIGLAAHTDTSLFTMLHQARKYGLQIFNEK 250
           L+P      T      L+LNSYP CPDP   +GLA HTD+SL T+L+Q    GL+I + +
Sbjct: 197 LVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQ 256

Query: 251 E---GWIPLARKSDTLIINIGDFLQIISNGRFHSIPHRVMIQETEESTMSMAFFYYPPSH 310
           E    WI +     +L++ +GD   IISNG+F S  HR ++ +T    +S A+F  PP +
Sbjct: 257 EEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHH-RVSAAYFAGPPKN 316

Query: 311 LNVLPYCKPLSETPQTPIYRGVNVKEYFAIKAKHSGKGI 344
           L + P     S+    PIYR +  +EY A KA H  K +
Sbjct: 317 LQIGPL---TSDKNHPPIYRRLIWEEYLAAKATHFNKAL 344

[Close]

BLAST of Cp4.1LG11g05740 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 5.2e-44
Identity = 100/297 (33.67%), Postives = 155/297 (52.19%), Query Frame = 0


Query: 47  IPVVDL-------NNDNVSEL--IGKACEKWGMFQLINHGISKTLIAQTEEVARCLFALP 106
           IPV+DL       N+D   E+  + +ACE WG FQ+INHGI   ++   EEVA   F +P
Sbjct: 55  IPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMP 114

Query: 107 KSQKMKTLQAPGNPTGYSMAGISKYYEKLMWHEGFTI-LDSPVDSFKKLWPSNYQRFCDI 166
             +K K    PG   GY  A I    +KL W   F + +  P     KLWPS   RF + 
Sbjct: 115 LEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSES 174

Query: 167 MEEYRLEMKALADKLISLIFKFLGISDEEMVKLLPYIDPNTGKPHMALRLNSYPPCPDPS 226
           +E Y  E++ L  +L+  I   LG+ +E   ++        G+   A+R+N YPPC  P 
Sbjct: 175 LEGYSKEIRELCKRLLKYIAISLGLKEERFEEMF-------GEAVQAVRMNYYPPCSSPD 234

Query: 227 KVIGLAAHTDTSLFTMLHQARK--YGLQIFNEKEGWIPLARKSDTLIINIGDFLQIISNG 286
            V+GL+ H+D S  T+L Q++    GLQI  +   W+P+    + L+INIGD ++++SNG
Sbjct: 235 LVLGLSPHSDGSALTVLQQSKNSCVGLQILKDNT-WVPVKPLPNALVINIGDTIEVLSNG 294

Query: 287 RFHSIPHRVMIQETEESTMSMAFFYYPPSHLNVLPYCKPLSETPQTPIYRGVNVKEY 332
           ++ S+ HR  +   E+  +++  FY P   + + P  + + +      YR  N  +Y
Sbjct: 295 KYKSVEHRA-VTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDY 342

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g05740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	1.7e-76	41.25	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q39103	1.0e-68	38.32	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9ZT84	7.5e-64	35.82	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q3I409	3.7e-63	36.52	Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum OX=4565 GN=GA3ox2-3 PE=2...	[more]
Q9SVS8	1.4e-62	37.76	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of Cp4.1LG11g05740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545777.1	4.62e-258	100.00	gibberellin 3-beta-dioxygenase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6598831.1	1.71e-244	94.54	Gibberellin 3-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022997424.1	2.42e-244	94.54	gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima]	[more]
XP_022929631.1	1.10e-240	93.10	gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
XP_023545778.1	1.50e-238	92.24	gibberellin 3-beta-dioxygenase 1-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cp4.1LG11g05740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KBB1	1.17e-244	94.54	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149234...	[more]
A0A6J1ENP0	5.34e-241	93.10	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436...	[more]
A0A6J1K7G4	5.23e-216	83.33	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149235...	[more]
A0A6J1ENA0	2.48e-214	82.76	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436...	[more]
A0A6J1ESR0	2.37e-200	77.39	gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436...	[more]

BLAST of Cp4.1LG11g05740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15550.1	7.2e-70	38.32	gibberellin 3-oxidase 1	[more]
AT1G80340.1	5.3e-65	35.82	gibberellin 3-oxidase 2	[more]
AT1G80330.1	1.0e-63	36.84	gibberellin 3-oxidase 4	[more]
AT4G21690.1	1.0e-63	37.76	gibberellin 3-oxidase 3	[more]
AT3G21420.1	5.2e-44	33.67	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 61..78 score: 43.94 coord: 224..245 score: 38.84 coord: 260..286 score: 27.95
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 9..342
None	No IPR available	PANTHER	PTHR47990:SF105	GIBBERELLIN 2-BETA-DIOXYGENASE	coord: 9..342
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 14..340
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..148 e-value: 1.5E-21 score: 77.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 10..333 e-value: 3.5E-76 score: 258.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 201..299 e-value: 6.9E-24 score: 84.3
IPR001261	ArgE/DapE/ACY1/CPG2/YscS, conserved site	PROSITE	PS00758	ARGE_DAPE_CPG2_1	coord: 219..228
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 199..301 score: 9.618827

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g05740.1	Cp4.1LG11g05740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding