Cp4.1LG11g04550 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g04550
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein DETOXIFICATION
LocationCp4.1LG11: 2582256 .. 2585297 (-)
RNA-Seq ExpressionCp4.1LG11g04550
SyntenyCp4.1LG11g04550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGATTTGAGAAACGATTGCGATTACAAGTTTTCGCATTGCTGGCCTATATATATATATATGTGTGTGTATATATATATACCCCTGCAGCTTAAGGGCTATACAGAATTGTTGTTCTTTTAGTCGTAAAAGGGCAGGGCAGGGCAGGGCAGGGCTGCTCATCGAACCCAGATGGAGGCCCATGTGAAGGCGGATGCGGCGGCGGATCTTAACCAGGCGTTGATTGGAAGGGATGAAGATCGACCTCGTCGGTCGGAGGAGGTATGGTCGAAGTTATGGGTTGAAACCCAGAAGCTATGGCTAATTGTTGGCCCGACTATTTTCGCCCGTGTTGCGACTTTCTCTATGAACATTATAACCCAAGCGTTTGCGGGTCATCTTGGTGATGTTGAACTGGCTTCCATTTCGATTGCTAATACTGTTATTGTTGGGTTCAATTTTGGGCTATTGGTATGGAATTTGGAGATGATTTTTTGTTATTTGAAATGGGTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGAATATGTTTGTTTTGAAAATTTTCAGTTGGGTATGGCGAGTGCATTGGAGACGTTATGTGGGCAAGCGTATGGAGCTAAGAGGTTTCATATGTTGGGGATTTACTTGCAGCGGTCATGGATAATGCTGTTCTTGTGTTGCTTTTTGTTATTACCCTTTTATGTTTATGCTACTCCGGTTCTGAAGATGCTGGGACAGGCGGATGATGTGGCGGAGCTATCTGGGGTGGTGGCGATTTGGTTAATACCACTTCATTTTAGCTTTGCGTTTCAGTTTCCATTGCAGGTGTTCTTGCAGTGCCAGCATAAGACACTTGTGATTGCTTGGGTGTCTTTGGTGGGGTTGGTGGTGAATGTTGTCACTAGTTGGCTATTCATTTATGAGTTGCAGCTTGGTGTCATTGGGGCTGCTATTGCTCTTGATATTTCTTGGTGGGTTTTGGGTTTGGGGTTGTATTTCTATACTGTTGGGGGTTGGTGTCCTTTGACATGGACTGGATTCTCTGTTAAAGCTTTTCATGGCCTTTGGGAGTTTCTTAAACTCTCCATTGCTGCTGGCCTCATGCTCTGGTAATTCCTATGTTCTTAACATCTCAAATATCATTGCTCCATAACTTTAGTTATTAATCAATTTGTTTCTCAATTGGGTTATGGCAGCTTGGAGAATTGGTACTTTCGAATCCTCGTATTGATGACCGGAAATCTCGAAAATGCTACGATCGCTGTTGATGCCTTATCCGTGTGGTATGTGAAATAGTAACCTAACTTTTCAAGCTCATACCTAGCAGATATTGTCCTCTTTAGGCTCGAGCTTCCTCTCAAAGTTTTTAAATCGTGTCTTTAGGGAGAGGTTTCCACACCCTGATGAGCAGATATTGTCCTCCTTAGGCTTGAACTTCCTCTCAAAGTTTTTAAATCGCGTCTTTAGGGAGAGTTTTCCACACCCTGATGAAGAACATTTCGTTCTCCTCTACGACGGATGTAGAATCTCACAATTCACTTCCATCGGGGCCTAGCATCCTCGCTGGCTCTCTCTCTCTGTTCCCCTTCATGGGTCCCACATGGATCGGAGAAGTAAACAAGTGCGAGCGAGAACATTGGCCCCAAAGGGGGTCGAGTGTGAGATCCGGTTAATGGTAGAGTAGAACGAAACGTTCTTCATAAGGATGTGAAAATCTCTCCCTAAGAAACGCCTTGTAGAACCCGAAAGGAAAAACCCAGAGACAATATCTGATCTTTAAGCATTTATGATCTTCCGAACGCTCATTATATCATTACGATTATCATTAATTTGTGGTTGAAACTTGTGCAGCATGAGCATCAATGGGTGGGAAATGATGATCCCTGTAGCCTTCTTAGCTGGTGTCGGGTAATACCCACTTGACAAAAACCAACACTTTTTAGCTTTTTCACAAGCAATGAATGAATCACTAAAACCTCTTTAATCCAAATGGAACAGAGTAAGAGTAGCAAACGAGCTAGGAGCTGGCAACGGGAAAGCAGCCAAATTTGCAACAATCGTCTCAGTGGCACAGTCAACAGTGATTGGAATAGCAATATGTCTTGTCATAGTCCTATTTCATGGCAAGATAGCACTGATCTTCTCAAGCAGCACAGACGTAGTGGAAGCAGTGGATTCCCTCTCCAATCTGCTAGCCATTACCATCCTCTTAAACAGCATCCAACCAGTTCTTTCAGGTTCATCCCTCTCTCCCAAACCAATCAAAACTGATACACATTCTGTTCATGTTCAAATATTGAAGACATTAGATGGGATATTGCAGGCGTGGCTGTTGGATCAGGCTGGCAAGCTTGGGTAGCATACATAAACCTTGGATGCTATTATGGTATAGGTCTCCCACTTGGTTTCATCATGCAATGGTATTACAAATCGGGCGTATCGGTACGTGCTTCCTCAACTACTACAACTATGTTCTTCGAATCGATTACGACGACTGACTCGTTTGAATTTACTGGAACAGGGCATTTGGGGAGGGATGTTGTTTGGAGGAAGTGCGTTTCAAACTCTGATATTGATCATCATAACAATTAGAACAGACTGGGAAAAAGAGGTAAAAGTTAATGGAATTGGTGTGTTTATAATGGAAGTGTTTTAATGGCAGCAGCTTAATTTGATTCATTTGGTGCTTGAATTTAGGCTGAGAATGCATTGAAGCACGTAGAGGAGTGGTCGACACCTCAGGACAATGAGAAGCTTCTTCTGGCGTAATAATAATATAATATTTTAAGTTGTAAGGGGTGGGGATGGAACTGGAATAATAATTATCCAAAATTAATAAATTGGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTAATTTTTTATATTCAGATGTATTAGTGAGAGAGATAGTGTGTAGATTGACTCGGAGTGCTTGGGTTCGAGATTTAATGAAAACACGTTGATTGTTTTTATGTTAATTTTTGTCTTCATTACCATTGTTGTAACGGAAGGGGAACAAAATATAAAATGAT

mRNA sequence

AAGGATTTGAGAAACGATTGCGATTACAAGTTTTCGCATTGCTGGCCTATATATATATATATGTGTGTGTATATATATATACCCCTGCAGCTTAAGGGCTATACAGAATTGTTGTTCTTTTAGTCGTAAAAGGGCAGGGCAGGGCAGGGCAGGGCTGCTCATCGAACCCAGATGGAGGCCCATGTGAAGGCGGATGCGGCGGCGGATCTTAACCAGGCGTTGATTGGAAGGGATGAAGATCGACCTCGTCGGTCGGAGGAGGTATGGTCGAAGTTATGGGTTGAAACCCAGAAGCTATGGCTAATTGTTGGCCCGACTATTTTCGCCCGTGTTGCGACTTTCTCTATGAACATTATAACCCAAGCGTTTGCGGGTCATCTTGGTGATGTTGAACTGGCTTCCATTTCGATTGCTAATACTGTTATTGTTGGGTTCAATTTTGGGCTATTGTTGGGTATGGCGAGTGCATTGGAGACGTTATGTGGGCAAGCGTATGGAGCTAAGAGGTTTCATATGTTGGGGATTTACTTGCAGCGGTCATGGATAATGCTGTTCTTGTGTTGCTTTTTGTTATTACCCTTTTATGTTTATGCTACTCCGGTTCTGAAGATGCTGGGACAGGCGGATGATGTGGCGGAGCTATCTGGGGTGGTGGCGATTTGGTTAATACCACTTCATTTTAGCTTTGCGTTTCAGTTTCCATTGCAGGTGTTCTTGCAGTGCCAGCATAAGACACTTGTGATTGCTTGGGTGTCTTTGGTGGGGTTGGTGGTGAATGTTGTCACTAGTTGGCTATTCATTTATGAGTTGCAGCTTGGTGTCATTGGGGCTGCTATTGCTCTTGATATTTCTTGGTGGGTTTTGGGTTTGGGGTTGTATTTCTATACTGTTGGGGGTTGGTGTCCTTTGACATGGACTGGATTCTCTGTTAAAGCTTTTCATGGCCTTTGGGAGTTTCTTAAACTCTCCATTGCTGCTGGCCTCATGCTCTGCATGAGCATCAATGGGTGGGAAATGATGATCCCTGTAGCCTTCTTAGCTGGTGTCGGAGTAAGAGTAGCAAACGAGCTAGGAGCTGGCAACGGGAAAGCAGCCAAATTTGCAACAATCGTCTCAGTGGCACAGTCAACAGTGATTGGAATAGCAATATGTCTTGTCATAGTCCTATTTCATGGCAAGATAGCACTGATCTTCTCAAGCAGCACAGACGTAGTGGAAGCAGTGGATTCCCTCTCCAATCTGCTAGCCATTACCATCCTCTTAAACAGCATCCAACCAGTTCTTTCAGGCGTGGCTGTTGGATCAGGCTGGCAAGCTTGGGTAGCATACATAAACCTTGGATGCTATTATGGTATAGGTCTCCCACTTGGTTTCATCATGCAATGGTATTACAAATCGGGCGTATCGGGCATTTGGGGAGGGATGTTGTTTGGAGGAAGTGCGTTTCAAACTCTGATATTGATCATCATAACAATTAGAACAGACTGGGAAAAAGAGGCTGAGAATGCATTGAAGCACGTAGAGGAGTGGTCGACACCTCAGGACAATGAGAAGCTTCTTCTGGCGTAATAATAATATAATATTTTAAGTTGTAAGGGGTGGGGATGGAACTGGAATAATAATTATCCAAAATTAATAAATTGGTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTAATTTTTTATATTCAGATGTATTAGTGAGAGAGATAGTGTGTAGATTGACTCGGAGTGCTTGGGTTCGAGATTTAATGAAAACACGTTGATTGTTTTTATGTTAATTTTTGTCTTCATTACCATTGTTGTAACGGAAGGGGAACAAAATATAAAATGAT

Coding sequence (CDS)

ATGGAGGCCCATGTGAAGGCGGATGCGGCGGCGGATCTTAACCAGGCGTTGATTGGAAGGGATGAAGATCGACCTCGTCGGTCGGAGGAGGTATGGTCGAAGTTATGGGTTGAAACCCAGAAGCTATGGCTAATTGTTGGCCCGACTATTTTCGCCCGTGTTGCGACTTTCTCTATGAACATTATAACCCAAGCGTTTGCGGGTCATCTTGGTGATGTTGAACTGGCTTCCATTTCGATTGCTAATACTGTTATTGTTGGGTTCAATTTTGGGCTATTGTTGGGTATGGCGAGTGCATTGGAGACGTTATGTGGGCAAGCGTATGGAGCTAAGAGGTTTCATATGTTGGGGATTTACTTGCAGCGGTCATGGATAATGCTGTTCTTGTGTTGCTTTTTGTTATTACCCTTTTATGTTTATGCTACTCCGGTTCTGAAGATGCTGGGACAGGCGGATGATGTGGCGGAGCTATCTGGGGTGGTGGCGATTTGGTTAATACCACTTCATTTTAGCTTTGCGTTTCAGTTTCCATTGCAGGTGTTCTTGCAGTGCCAGCATAAGACACTTGTGATTGCTTGGGTGTCTTTGGTGGGGTTGGTGGTGAATGTTGTCACTAGTTGGCTATTCATTTATGAGTTGCAGCTTGGTGTCATTGGGGCTGCTATTGCTCTTGATATTTCTTGGTGGGTTTTGGGTTTGGGGTTGTATTTCTATACTGTTGGGGGTTGGTGTCCTTTGACATGGACTGGATTCTCTGTTAAAGCTTTTCATGGCCTTTGGGAGTTTCTTAAACTCTCCATTGCTGCTGGCCTCATGCTCTGCATGAGCATCAATGGGTGGGAAATGATGATCCCTGTAGCCTTCTTAGCTGGTGTCGGAGTAAGAGTAGCAAACGAGCTAGGAGCTGGCAACGGGAAAGCAGCCAAATTTGCAACAATCGTCTCAGTGGCACAGTCAACAGTGATTGGAATAGCAATATGTCTTGTCATAGTCCTATTTCATGGCAAGATAGCACTGATCTTCTCAAGCAGCACAGACGTAGTGGAAGCAGTGGATTCCCTCTCCAATCTGCTAGCCATTACCATCCTCTTAAACAGCATCCAACCAGTTCTTTCAGGCGTGGCTGTTGGATCAGGCTGGCAAGCTTGGGTAGCATACATAAACCTTGGATGCTATTATGGTATAGGTCTCCCACTTGGTTTCATCATGCAATGGTATTACAAATCGGGCGTATCGGGCATTTGGGGAGGGATGTTGTTTGGAGGAAGTGCGTTTCAAACTCTGATATTGATCATCATAACAATTAGAACAGACTGGGAAAAAGAGGCTGAGAATGCATTGAAGCACGTAGAGGAGTGGTCGACACCTCAGGACAATGAGAAGCTTCTTCTGGCGTAA

Protein sequence

MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWSTPQDNEKLLLA
Homology
BLAST of Cp4.1LG11g04550 vs. ExPASy Swiss-Prot
Match: Q9FKQ1 (Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 7.7e-157
Identity = 288/468 (61.54%), Postives = 349/468 (74.57%), Query Frame = 0

Query: 16  ALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVEL 75
           AL+          E +  ++ VET+KLW IVGP IF+RV T+SM +ITQAFAGHLGD+EL
Sbjct: 15  ALLKSPHTAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLGDLEL 74

Query: 76  ASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLL 135
           A+ISI N V VGFNFGLLLGMASALETLCGQA+GAK++HMLG+Y+QRSWI+LF CC LLL
Sbjct: 75  AAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCCVLLL 134

Query: 136 PFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVS 195
           P Y++ TPVLK LGQ DD+AELSGVVAIW+IPLHF+F   FPLQ FLQCQ K  V A+ +
Sbjct: 135 PTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVTAYAA 194

Query: 196 LVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKA 255
            V LVV+++  WLF+  L+LGV+G    + ISWWV  L L  Y+  G CPLTWTG S +A
Sbjct: 195 AVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTWTGLSSEA 254

Query: 256 FHGLWEFLKLSIAAGLML-----------------------------CMSINGWEMMIPV 315
             GLWEFLKLS ++G+ML                             CM+INGWEMMIP+
Sbjct: 255 LTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAINGWEMMIPL 314

Query: 316 AFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTD 375
           AF AG GVRVANELGAGNGK A+FATIVSV QS +IG+   ++I+L H +IA IFSSS  
Sbjct: 315 AFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFSSSVA 374

Query: 376 VVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWY 435
           V++AV+ LS LLA T+LLNS+QPVLSGVAVGSGWQ++VAYINLGCYY IG+PLGF+M W 
Sbjct: 375 VLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFLMGWG 434

Query: 436 YKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWS 455
           +K GV GIWGGM+FGG+A QT+IL  IT+R DWEKEA+ A   + +WS
Sbjct: 435 FKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of Cp4.1LG11g04550 vs. ExPASy Swiss-Prot
Match: Q1PDX9 (Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.6e-146
Identity = 262/449 (58.35%), Postives = 336/449 (74.83%), Query Frame = 0

Query: 34  KLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVELASISIANTVIVGFNFGLL 93
           ++W+ET+K+W IVGP+IF  +AT+S+ IITQAFAGHLGD+ELA+ISI N   +GFN+GLL
Sbjct: 34  EIWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFTLGFNYGLL 93

Query: 94  LGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLLPFYVYATPVLKMLGQADD 153
           LGMASALETLCGQA+GA+ ++MLG+Y+QR WI+LFLCC LLLP Y++ATP+LK +GQ+DD
Sbjct: 94  LGMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPILKFIGQSDD 153

Query: 154 VAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYEL 213
           +AEL+G +A+W+IP+HF+FAF FPL  FLQCQ K  VIA  + V L V+++  W F+Y  
Sbjct: 154 IAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILVCWFFVYGY 213

Query: 214 QLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLML 273
           +LG+IG   ++++ WW+    L+ Y+  G C LTWTGFS +AF GL E  KLS ++G+ML
Sbjct: 214 KLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKLSASSGIML 273

Query: 274 -----------------------------CMSINGWEMMIPVAFLAGVGVRVANELGAGN 333
                                        CMS+NGWEMMIP+AF AG GVRVANELGAGN
Sbjct: 274 CLENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRVANELGAGN 333

Query: 334 GKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTDVVEAVDSLSNLLAITILL 393
           GK A+FATIVS+  S +IG+   ++IV+FH +I  IFSSS  V+ AVD+LS LLA T+LL
Sbjct: 334 GKGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLL 393

Query: 394 NSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSA 453
           NS+QPVLSGVAVGSGWQ++VAYINLGCYY IGLP G  M W +K GV GIW GM+FGG+A
Sbjct: 394 NSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVKGIWAGMIFGGTA 453

BLAST of Cp4.1LG11g04550 vs. ExPASy Swiss-Prot
Match: Q9FNC1 (Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.6e-144
Identity = 265/454 (58.37%), Postives = 333/454 (73.35%), Query Frame = 0

Query: 30  EVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVELASISIANTVIVGFN 89
           E+  ++W+ET+KLW IVGP IF RV T  + +ITQAFAGHLG++ELA+ISI N VI+GFN
Sbjct: 32  EIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGELELAAISIVNNVIIGFN 91

Query: 90  FGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLLPFYVYATPVLKMLG 149
           + L +GMA+ALETLCGQA+GAK++ M G+YLQRSWI+LFL   LLLP Y++ATP+LK +G
Sbjct: 92  YSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSILLLPMYIFATPILKFMG 151

Query: 150 QADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVSLVGLVVNVVTSWLF 209
           Q DD+AELSG++++W IP HFSFAF FP+  FLQCQ K  VIA  S V LVV++   WLF
Sbjct: 152 QPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIAISSGVSLVVHIFVCWLF 211

Query: 210 IYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKAFHGLWEFLKLSIAA 269
           +Y L+LGVIG     ++SWW+    L+ YT  G CPLTWTGFS+++F  LWEF KLS ++
Sbjct: 212 VYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFSMESFTRLWEFTKLSASS 271

Query: 270 GLM-----------------------------LCMSINGWEMMIPVAFLAGVGVRVANEL 329
           G+M                             +CMSING EMM+P+AF AG  VRVANEL
Sbjct: 272 GIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMMVPLAFFAGTSVRVANEL 331

Query: 330 GAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTDVVEAVDSLSNLLAI 389
           GAGNGK A+FA I+SV QS +IGI I ++I     +I  +FSSS  V++AV++LS LL+ 
Sbjct: 332 GAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSSSETVLKAVNNLSILLSF 391

Query: 390 TILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWYYKSGVSGIWGGMLF 449
            ILLNS+QPVLSGVAVGSGWQ+ VA+INLGCYY IGLPLG +M W +K GV GIW GM+F
Sbjct: 392 AILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVMGWMFKFGVKGIWAGMIF 451

Query: 450 GGSAFQTLILIIITIRTDWEKEAENALKHVEEWS 455
           GG+  QTLILI IT+R DWEKEA+NA   V +WS
Sbjct: 452 GGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of Cp4.1LG11g04550 vs. ExPASy Swiss-Prot
Match: Q9SX83 (Protein DETOXIFICATION 33 OS=Arabidopsis thaliana OX=3702 GN=DTX33 PE=2 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.8e-106
Identity = 210/458 (45.85%), Postives = 291/458 (63.54%), Query Frame = 0

Query: 26  RRSEEVWSK-LWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVELASISIANTV 85
           + + +VW+K    E+++LW + GP IF  ++ +S+  +TQ F+G LG++ELA++S+ N+V
Sbjct: 22  KSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGALTQTFSGRLGELELAAVSVENSV 81

Query: 86  IVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLLPFYVYATPV 145
           I G  FG++LGM SALETLCGQAYGA +  M+GIY+QRSW++LF     LLP Y++A P+
Sbjct: 82  ISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQRSWVILFTTALFLLPVYIWAPPI 141

Query: 146 LKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVSLVGLVVNVV 205
           L   G+A  +++ +G  A+W+IP  F++A  FP+Q FLQ Q K LV+AW+S V LV++ V
Sbjct: 142 LSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFLQSQRKVLVMAWISGVVLVIHAV 201

Query: 206 TSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKAFHGLWEFLK 265
            SWLFI   + G++GAAI L+ SWW++ +G   Y +       WTGFS+ AF  L+ F+K
Sbjct: 202 FSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILITKSDGAWTGFSMLAFRDLYGFVK 261

Query: 266 LSIAAGLML-----------------------------CMSINGWEMMIPVAFLAGVGVR 325
           LS+A+ LML                             CM+I GW  MI + F A + VR
Sbjct: 262 LSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISICMNIEGWTAMISIGFNAAISVR 321

Query: 326 VANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTDVVEAVDSLS 385
           V+NELGAGN   AKF+ IV    ST+IGI   +V++        +F+SS  V      ++
Sbjct: 322 VSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLATKDSFPYLFTSSEAVAAETTRIA 381

Query: 386 NLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWYYKSGVSGIW 445
            LL  T+LLNS+QPVLSGVAVG+GWQA VAY+N+ CYY IGLP G ++ +    GV GIW
Sbjct: 382 VLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYYIIGLPAGLVLGFTLDLGVQGIW 441

Query: 446 GGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEW 454
           GGM+  G   QTLILI I   T+W KEAE A   V+ W
Sbjct: 442 GGMV-AGICLQTLILIGIIYFTNWNKEAEQAESRVQRW 478

BLAST of Cp4.1LG11g04550 vs. ExPASy Swiss-Prot
Match: Q8W488 (Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 3.6e-106
Identity = 206/486 (42.39%), Postives = 304/486 (62.55%), Query Frame = 0

Query: 7   ADAAADLNQALIGRDEDRPRRSEE---VWSKLWVETQKLWLIVGPTIFARVATFSMNIIT 66
           A    +L  AL+ +  +     ++   +  K+W+E++KLW++  P IF R +TF ++II+
Sbjct: 2   AGGGGELTAALLKKTAENGGEEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIIS 61

Query: 67  QAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRS 126
           Q+F GHLG +ELA+ SI  TV++ F+ G+LLGMASALETLCGQAYGAK+ HMLGIYLQRS
Sbjct: 62  QSFIGHLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRS 121

Query: 127 WIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQ 186
           WI+L  C   L P Y+++ P+L  LGQ + +  ++ ++A+W+I ++FSF   F  Q+FLQ
Sbjct: 122 WIVLTGCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQ 181

Query: 187 CQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGW 246
            Q K  +IA+V+ V L V+V  SWL +     G+ GA  +  +++W+  +    +   G 
Sbjct: 182 AQSKNKIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGG 241

Query: 247 CPLTWTGFSVKAFHGLWEFLKLSIAAG-----------------------------LMLC 306
           C  TW GFS+ AF  LW   KLS+++G                             L +C
Sbjct: 242 CKDTWRGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAIC 301

Query: 307 MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFH 366
           ++ING EMMI + FLA   VRV+NELG+GN K AKFAT+ +V  S  +GI +  V +   
Sbjct: 302 LNINGLEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLR 361

Query: 367 GKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYG 426
           G+++ IF++S  V   V  LS LLA +IL+NS+QPVLSGVAVG+GWQ +V Y+NL CYY 
Sbjct: 362 GRVSYIFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYL 421

Query: 427 IGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWS 461
           +G+P+G I+ +     V G+W GMLF G   QT +L ++T+RTDW+++   +L+ +  W 
Sbjct: 422 VGIPIGIILGYVVGLQVKGVWIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRWV 481

BLAST of Cp4.1LG11g04550 vs. NCBI nr
Match: XP_023545612.1 (protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 893 bits (2308), Expect = 0.0
Identity = 465/494 (94.13%), Postives = 465/494 (94.13%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60
           MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN
Sbjct: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60

Query: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120
           IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL
Sbjct: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120

Query: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180
           QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV
Sbjct: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180

Query: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240
           FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV
Sbjct: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240

Query: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-------------------------- 300
           GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC                          
Sbjct: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDAL 300

Query: 301 ---MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360
              MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV
Sbjct: 301 SVCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360

Query: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420
           LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC
Sbjct: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420

Query: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 465
           YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE
Sbjct: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 480

BLAST of Cp4.1LG11g04550 vs. NCBI nr
Match: KAG6598709.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 878 bits (2268), Expect = 0.0
Identity = 457/494 (92.51%), Postives = 461/494 (93.32%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60
           MEA VKADAAADLNQALI RDEDRPRRSEE+WSKLWVETQKLWLIVGPTIFARVATFSMN
Sbjct: 1   MEALVKADAAADLNQALIRRDEDRPRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMN 60

Query: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120
           +ITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL
Sbjct: 61  VITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120

Query: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180
           QRSWIMLFLCCFLLLPFYVYATPVLKMLGQA+DVAELSGVVAIWLIPLHFSFAFQFPLQV
Sbjct: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQV 180

Query: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240
           FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV
Sbjct: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240

Query: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-------------------------- 300
           GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC                          
Sbjct: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDAL 300

Query: 301 ---MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360
              MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV
Sbjct: 301 SVCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360

Query: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420
           LFHGKIALIFSSS DVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC
Sbjct: 361 LFHGKIALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420

Query: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 465
           YYGIGLPLGF+MQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAE ALKHVE
Sbjct: 421 YYGIGLPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAEKALKHVE 480

BLAST of Cp4.1LG11g04550 vs. NCBI nr
Match: KAG7029651.1 (Protein DETOXIFICATION 27 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 455/494 (92.11%), Postives = 460/494 (93.12%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60
           MEA VKADAAADLN+ALI R EDRPRRSEE+WSKLWVETQKLWLIVGPTIFARVATFSMN
Sbjct: 1   MEALVKADAAADLNEALIRRGEDRPRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMN 60

Query: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120
           +ITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL
Sbjct: 61  VITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120

Query: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180
           QRSWIMLFLCCFLLLPFYVYATPVLKMLGQA+DVAELSGVVAIWLIPLHFSFAFQFPLQV
Sbjct: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQV 180

Query: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240
           FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV
Sbjct: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240

Query: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-------------------------- 300
           GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC                          
Sbjct: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDAL 300

Query: 301 ---MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360
              MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV
Sbjct: 301 SVCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360

Query: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420
           LFHGKIALIFSSS DVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC
Sbjct: 361 LFHGKIALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420

Query: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 465
           YYGIGLPLGF+MQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAE ALKHVE
Sbjct: 421 YYGIGLPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAEKALKHVE 480

BLAST of Cp4.1LG11g04550 vs. NCBI nr
Match: XP_022962463.1 (protein DETOXIFICATION 27-like [Cucurbita moschata] >XP_022962464.1 protein DETOXIFICATION 27-like [Cucurbita moschata])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 455/494 (92.11%), Postives = 460/494 (93.12%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60
           MEA VKADAAADLNQALI RDEDRPRRSEE+WSKLWVETQKLWLIVGPTIFARVATFSMN
Sbjct: 1   MEALVKADAAADLNQALIRRDEDRPRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMN 60

Query: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120
           +ITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL
Sbjct: 61  VITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120

Query: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180
           QRSWIMLFLCCFLLLPFYVYATPVLKMLGQA+DVAELSGVVAIWLIPLHFSFAFQFPLQV
Sbjct: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQV 180

Query: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240
           FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV
Sbjct: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240

Query: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-------------------------- 300
           GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC                          
Sbjct: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDAL 300

Query: 301 ---MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360
              MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAIC+VIV
Sbjct: 301 SVCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICVVIV 360

Query: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420
           LFHGKIALIFSSS DVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC
Sbjct: 361 LFHGKIALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420

Query: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 465
           YYGIGLPLGF+MQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEA  ALKHVE
Sbjct: 421 YYGIGLPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAVKALKHVE 480

BLAST of Cp4.1LG11g04550 vs. NCBI nr
Match: XP_022997119.1 (protein DETOXIFICATION 27-like [Cucurbita maxima])

HSP 1 Score: 867 bits (2239), Expect = 1.55e-315
Identity = 451/494 (91.30%), Postives = 458/494 (92.71%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60
           MEA VKADAA+DLNQ LI R EDR RRSEE+WSKLWVETQKLWLIVGPTIFARVATFSMN
Sbjct: 1   MEALVKADAASDLNQPLIRRGEDRTRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMN 60

Query: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120
           +ITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL
Sbjct: 61  VITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120

Query: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180
           QRSWIMLFLCCFLLLPFYVYATPVLKMLGQA+DVAELSGVVAIWLIPLHFSFAFQFPLQV
Sbjct: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQV 180

Query: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240
           FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV
Sbjct: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240

Query: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-------------------------- 300
           GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC                          
Sbjct: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDAL 300

Query: 301 ---MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360
              MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV
Sbjct: 301 SVCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360

Query: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420
           LFHGKIALIFSSS DVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC
Sbjct: 361 LFHGKIALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420

Query: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 465
           YYGIGLPLGF+MQWYYKSGVSGIWGGMLFGGSAFQTLIL+IITIRTDWEKEAE ALKHVE
Sbjct: 421 YYGIGLPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILMIITIRTDWEKEAEKALKHVE 480

BLAST of Cp4.1LG11g04550 vs. ExPASy TrEMBL
Match: A0A6J1HEV9 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111462892 PE=3 SV=1)

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 455/494 (92.11%), Postives = 460/494 (93.12%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60
           MEA VKADAAADLNQALI RDEDRPRRSEE+WSKLWVETQKLWLIVGPTIFARVATFSMN
Sbjct: 1   MEALVKADAAADLNQALIRRDEDRPRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMN 60

Query: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120
           +ITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL
Sbjct: 61  VITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120

Query: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180
           QRSWIMLFLCCFLLLPFYVYATPVLKMLGQA+DVAELSGVVAIWLIPLHFSFAFQFPLQV
Sbjct: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQV 180

Query: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240
           FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV
Sbjct: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240

Query: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-------------------------- 300
           GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC                          
Sbjct: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDAL 300

Query: 301 ---MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360
              MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAIC+VIV
Sbjct: 301 SVCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICVVIV 360

Query: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420
           LFHGKIALIFSSS DVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC
Sbjct: 361 LFHGKIALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420

Query: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 465
           YYGIGLPLGF+MQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEA  ALKHVE
Sbjct: 421 YYGIGLPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAVKALKHVE 480

BLAST of Cp4.1LG11g04550 vs. ExPASy TrEMBL
Match: A0A6J1KAJ6 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111492126 PE=3 SV=1)

HSP 1 Score: 867 bits (2239), Expect = 7.53e-316
Identity = 451/494 (91.30%), Postives = 458/494 (92.71%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMN 60
           MEA VKADAA+DLNQ LI R EDR RRSEE+WSKLWVETQKLWLIVGPTIFARVATFSMN
Sbjct: 1   MEALVKADAASDLNQPLIRRGEDRTRRSEELWSKLWVETQKLWLIVGPTIFARVATFSMN 60

Query: 61  IITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120
           +ITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL
Sbjct: 61  VITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYL 120

Query: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQV 180
           QRSWIMLFLCCFLLLPFYVYATPVLKMLGQA+DVAELSGVVAIWLIPLHFSFAFQFPLQV
Sbjct: 121 QRSWIMLFLCCFLLLPFYVYATPVLKMLGQAEDVAELSGVVAIWLIPLHFSFAFQFPLQV 180

Query: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240
           FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV
Sbjct: 181 FLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTV 240

Query: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-------------------------- 300
           GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC                          
Sbjct: 241 GGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLCLENWYFRILVLMTGNLENATIAVDAL 300

Query: 301 ---MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360
              MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV
Sbjct: 301 SVCMSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIV 360

Query: 361 LFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420
           LFHGKIALIFSSS DVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC
Sbjct: 361 LFHGKIALIFSSSRDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGC 420

Query: 421 YYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVE 465
           YYGIGLPLGF+MQWYYKSGVSGIWGGMLFGGSAFQTLIL+IITIRTDWEKEAE ALKHVE
Sbjct: 421 YYGIGLPLGFVMQWYYKSGVSGIWGGMLFGGSAFQTLILMIITIRTDWEKEAEKALKHVE 480

BLAST of Cp4.1LG11g04550 vs. ExPASy TrEMBL
Match: A0A6J1BQ61 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004791 PE=3 SV=1)

HSP 1 Score: 744 bits (1921), Expect = 8.44e-268
Identity = 378/484 (78.10%), Postives = 418/484 (86.36%), Query Frame = 0

Query: 11  ADLNQALIGR-DEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGH 70
           +D NQ L+   DED   RS++ WSKLW ETQ+LW+IVGPTIFARVATF+MN+ITQ FAGH
Sbjct: 3   SDHNQPLLSNEDEDGKWRSQDFWSKLWRETQQLWVIVGPTIFARVATFTMNVITQVFAGH 62

Query: 71  LGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFL 130
           LGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++HMLGIYLQRSWI+LFL
Sbjct: 63  LGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSWIVLFL 122

Query: 131 CCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTL 190
           CCFLLLPFYVY TPVLK+LG  DDVAE SGVVAIWLIPLHFSFAFQFPLQ FLQCQHKT+
Sbjct: 123 CCFLLLPFYVYTTPVLKLLGHEDDVAEESGVVAIWLIPLHFSFAFQFPLQTFLQCQHKTM 182

Query: 191 VIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWT 250
           VIAWVSLVGL+VNVV+SW+ IYE +LGVIGAAIALD+SWWVLG GLYFYTVGGWCPLTWT
Sbjct: 183 VIAWVSLVGLIVNVVSSWVLIYEWELGVIGAAIALDVSWWVLGFGLYFYTVGGWCPLTWT 242

Query: 251 GFSVKAFHGLWEFLKLSIAAGLMLC-----------------------------MSINGW 310
           GFS++AFHGLWEFLKLS AAGLMLC                             MSINGW
Sbjct: 243 GFSIQAFHGLWEFLKLSTAAGLMLCLENWYFRILVLMTGNLENATTAVDALSVCMSINGW 302

Query: 311 EMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALI 370
           EMMIP+AF AG+GVRVANELGAGNGK AKFATIVSVAQS +IG+ IC+VI+ FH KIALI
Sbjct: 303 EMMIPIAFFAGIGVRVANELGAGNGKGAKFATIVSVAQSILIGVVICVVIMTFHDKIALI 362

Query: 371 FSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLG 430
           F+SS+ VVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQ+WVAYIN+GCYY IGLPLG
Sbjct: 363 FTSSSSVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQSWVAYINIGCYYIIGLPLG 422

Query: 431 FIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWSTPQDNE 464
           FIM+W + SGVSGIW GML+GG+A QT+ILIIITIRTDWEKEAE A  HV+ WS+P+ +E
Sbjct: 423 FIMEWVFHSGVSGIWAGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVDNWSSPRKDE 482

BLAST of Cp4.1LG11g04550 vs. ExPASy TrEMBL
Match: A0A6J1BQC7 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004792 PE=3 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 1.45e-259
Identity = 372/490 (75.92%), Postives = 415/490 (84.69%), Query Frame = 0

Query: 11  ADLNQALI------GRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQ 70
           ADLN+ L+      G D++  R     +SK+WVETQ+LW IVGPTIFARVA +SMN+ITQ
Sbjct: 2   ADLNRPLLISTSNNGGDDENGRSK---YSKVWVETQQLWAIVGPTIFARVANYSMNVITQ 61

Query: 71  AFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSW 130
            FAGHLGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++HMLGIYLQRSW
Sbjct: 62  VFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKYHMLGIYLQRSW 121

Query: 131 IMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQC 190
           I+LF CCFLLLPFYVYA PVLK LGQ D+VAE SG+VAIWLIPLHFSFAFQFPLQ FLQC
Sbjct: 122 IVLFFCCFLLLPFYVYAAPVLKWLGQDDEVAEESGLVAIWLIPLHFSFAFQFPLQTFLQC 181

Query: 191 QHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWC 250
           Q KTLVIAWVSL GL++NVV+SW+ IYE +LGVIGAAIALD+SWWVLG GLYFYTVGGWC
Sbjct: 182 QQKTLVIAWVSLAGLILNVVSSWVLIYEWELGVIGAAIALDLSWWVLGFGLYFYTVGGWC 241

Query: 251 PLTWTGFSVKAFHGLWEFLKLSIAAGLMLC-----------------------------M 310
           PLTWTGFS++AFHGLWEFLKLS AAGLMLC                             M
Sbjct: 242 PLTWTGFSIQAFHGLWEFLKLSAAAGLMLCLENWYFRILVLMTGNLENATIAVDALSVCM 301

Query: 311 SINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHG 370
           SINGWEMMIP+AF AGVGVRVANELGAGNGKAAKFATIVSVA+ST IGI IC +I++FHG
Sbjct: 302 SINGWEMMIPMAFFAGVGVRVANELGAGNGKAAKFATIVSVAESTGIGIVICAIIMIFHG 361

Query: 371 KIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGI 430
           KIALIF+SS+ VVEAVD+LSNLLAITILLNS+QPVLSGVAVGSGWQ+WVAYIN+GCYY I
Sbjct: 362 KIALIFTSSSRVVEAVDTLSNLLAITILLNSVQPVLSGVAVGSGWQSWVAYINIGCYYII 421

Query: 431 GLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWST 465
           GLPLGFIM+W + SGVSGIW GML+GG+A QT+ILIIITIRTDWEKEAE A  HVE WS+
Sbjct: 422 GLPLGFIMEWVFHSGVSGIWAGMLYGGTALQTIILIIITIRTDWEKEAEKAQMHVERWSS 481

BLAST of Cp4.1LG11g04550 vs. ExPASy TrEMBL
Match: A0A6J1BQ65 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004794 PE=3 SV=1)

HSP 1 Score: 712 bits (1838), Expect = 5.83e-255
Identity = 370/501 (73.85%), Postives = 421/501 (84.03%), Query Frame = 0

Query: 1   MEAHVKADAAADLNQALIGRDEDRPR-------RSEEVWSKLWVETQKLWLIVGPTIFAR 60
           M + ++ADA   LNQ LI   +D+ R       R E+  SKLWVETQ+LWLIVGP+IF+R
Sbjct: 1   MGSIIRADAEG-LNQPLIISSKDKSRATESGEDRREDFLSKLWVETQQLWLIVGPSIFSR 60

Query: 61  VATFSMNIITQAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRF 120
           VA +SMNIITQAFAGHLGDV+LASISIANTVIVGFNFGLLLGMASALETLCGQAYGAK++
Sbjct: 61  VAGYSMNIITQAFAGHLGDVQLASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKKY 120

Query: 121 HMLGIYLQRSWIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFA 180
           HMLGIYLQRSWI+L LCCFLLLPFY YATPVLK+LGQ DDVAE SGVVA+WLIPLHFSFA
Sbjct: 121 HMLGIYLQRSWIVLCLCCFLLLPFYFYATPVLKLLGQPDDVAEQSGVVALWLIPLHFSFA 180

Query: 181 FQFPLQVFLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGL 240
           FQFPLQ FLQ Q KT VIAWVSL+GL+VN+VTSW+ +Y  + GVIGAAIALDISWWVL  
Sbjct: 181 FQFPLQRFLQSQLKTQVIAWVSLLGLLVNIVTSWVLMYVWEFGVIGAAIALDISWWVLVF 240

Query: 241 GLYFYTVGGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLMLC------------------- 300
           GLY YTVGGWCPLTWTGFSV+AF GLW+F KLS AAGLMLC                   
Sbjct: 241 GLYIYTVGGWCPLTWTGFSVQAFQGLWDFTKLSAAAGLMLCSENWYYRILVLMTGNLKDA 300

Query: 301 ----------MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGI 360
                     MSINGWEMM+P+AF AG+GVRVANELGAGNGK AKFATIVSVAQSTVIG+
Sbjct: 301 TVAVDALSICMSINGWEMMVPLAFFAGIGVRVANELGAGNGKGAKFATIVSVAQSTVIGV 360

Query: 361 AICLVIVLFHGKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWV 420
            IC+VI++ H KIALIF+SS+ VVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQ+WV
Sbjct: 361 VICVVIMILHDKIALIFTSSSSVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQSWV 420

Query: 421 AYINLGCYYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAE 465
           AYIN+GCYY IGLPLGF+M+W + SGV GIWGGM+FGG+A QT+IL+IIT+RT+WE+EA+
Sbjct: 421 AYINIGCYYLIGLPLGFVMEWVFHSGVLGIWGGMIFGGTAIQTIILVIITMRTNWEQEAQ 480

BLAST of Cp4.1LG11g04550 vs. TAIR 10
Match: AT5G65380.1 (MATE efflux family protein )

HSP 1 Score: 555.1 bits (1429), Expect = 5.5e-158
Identity = 288/468 (61.54%), Postives = 349/468 (74.57%), Query Frame = 0

Query: 16  ALIGRDEDRPRRSEEVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVEL 75
           AL+          E +  ++ VET+KLW IVGP IF+RV T+SM +ITQAFAGHLGD+EL
Sbjct: 15  ALLKSPHTAEEDGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAGHLGDLEL 74

Query: 76  ASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLL 135
           A+ISI N V VGFNFGLLLGMASALETLCGQA+GAK++HMLG+Y+QRSWI+LF CC LLL
Sbjct: 75  AAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLFFCCVLLL 134

Query: 136 PFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVS 195
           P Y++ TPVLK LGQ DD+AELSGVVAIW+IPLHF+F   FPLQ FLQCQ K  V A+ +
Sbjct: 135 PTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKNRVTAYAA 194

Query: 196 LVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKA 255
            V LVV+++  WLF+  L+LGV+G    + ISWWV  L L  Y+  G CPLTWTG S +A
Sbjct: 195 AVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTWTGLSSEA 254

Query: 256 FHGLWEFLKLSIAAGLML-----------------------------CMSINGWEMMIPV 315
             GLWEFLKLS ++G+ML                             CM+INGWEMMIP+
Sbjct: 255 LTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAINGWEMMIPL 314

Query: 316 AFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTD 375
           AF AG GVRVANELGAGNGK A+FATIVSV QS +IG+   ++I+L H +IA IFSSS  
Sbjct: 315 AFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAWIFSSSVA 374

Query: 376 VVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWY 435
           V++AV+ LS LLA T+LLNS+QPVLSGVAVGSGWQ++VAYINLGCYY IG+PLGF+M W 
Sbjct: 375 VLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPLGFLMGWG 434

Query: 436 YKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWS 455
           +K GV GIWGGM+FGG+A QT+IL  IT+R DWEKEA+ A   + +WS
Sbjct: 435 FKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of Cp4.1LG11g04550 vs. TAIR 10
Match: AT5G10420.1 (MATE efflux family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-147
Identity = 262/449 (58.35%), Postives = 336/449 (74.83%), Query Frame = 0

Query: 34  KLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVELASISIANTVIVGFNFGLL 93
           ++W+ET+K+W IVGP+IF  +AT+S+ IITQAFAGHLGD+ELA+ISI N   +GFN+GLL
Sbjct: 34  EIWIETKKIWYIVGPSIFTGLATYSILIITQAFAGHLGDLELAAISIINNFTLGFNYGLL 93

Query: 94  LGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLLPFYVYATPVLKMLGQADD 153
           LGMASALETLCGQA+GA+ ++MLG+Y+QR WI+LFLCC LLLP Y++ATP+LK +GQ+DD
Sbjct: 94  LGMASALETLCGQAFGAREYYMLGVYMQRYWIILFLCCILLLPMYLFATPILKFIGQSDD 153

Query: 154 VAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVSLVGLVVNVVTSWLFIYEL 213
           +AEL+G +A+W+IP+HF+FAF FPL  FLQCQ K  VIA  + V L V+++  W F+Y  
Sbjct: 154 IAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLKNKVIAISAGVSLAVHILVCWFFVYGY 213

Query: 214 QLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKAFHGLWEFLKLSIAAGLML 273
           +LG+IG   ++++ WW+    L+ Y+  G C LTWTGFS +AF GL E  KLS ++G+ML
Sbjct: 214 KLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLTWTGFSSEAFTGLLELTKLSASSGIML 273

Query: 274 -----------------------------CMSINGWEMMIPVAFLAGVGVRVANELGAGN 333
                                        CMS+NGWEMMIP+AF AG GVRVANELGAGN
Sbjct: 274 CLENWYYKILMLMTGNLVNAKIAVDSLSICMSVNGWEMMIPLAFFAGTGVRVANELGAGN 333

Query: 334 GKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTDVVEAVDSLSNLLAITILL 393
           GK A+FATIVS+  S +IG+   ++IV+FH +I  IFSSS  V+ AVD+LS LLA T+LL
Sbjct: 334 GKGARFATIVSITLSLMIGLFFTVIIVIFHDQIGSIFSSSEAVLNAVDNLSVLLAFTVLL 393

Query: 394 NSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWYYKSGVSGIWGGMLFGGSA 453
           NS+QPVLSGVAVGSGWQ++VAYINLGCYY IGLP G  M W +K GV GIW GM+FGG+A
Sbjct: 394 NSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLPFGLTMGWIFKFGVKGIWAGMIFGGTA 453

BLAST of Cp4.1LG11g04550 vs. TAIR 10
Match: AT5G44050.1 (MATE efflux family protein )

HSP 1 Score: 513.5 bits (1321), Expect = 1.8e-145
Identity = 265/454 (58.37%), Postives = 333/454 (73.35%), Query Frame = 0

Query: 30  EVWSKLWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVELASISIANTVIVGFN 89
           E+  ++W+ET+KLW IVGP IF RV T  + +ITQAFAGHLG++ELA+ISI N VI+GFN
Sbjct: 32  EIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFAGHLGELELAAISIVNNVIIGFN 91

Query: 90  FGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLLPFYVYATPVLKMLG 149
           + L +GMA+ALETLCGQA+GAK++ M G+YLQRSWI+LFL   LLLP Y++ATP+LK +G
Sbjct: 92  YSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVLFLFSILLLPMYIFATPILKFMG 151

Query: 150 QADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVSLVGLVVNVVTSWLF 209
           Q DD+AELSG++++W IP HFSFAF FP+  FLQCQ K  VIA  S V LVV++   WLF
Sbjct: 152 QPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLKNSVIAISSGVSLVVHIFVCWLF 211

Query: 210 IYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKAFHGLWEFLKLSIAA 269
           +Y L+LGVIG     ++SWW+    L+ YT  G CPLTWTGFS+++F  LWEF KLS ++
Sbjct: 212 VYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLTWTGFSMESFTRLWEFTKLSASS 271

Query: 270 GLM-----------------------------LCMSINGWEMMIPVAFLAGVGVRVANEL 329
           G+M                             +CMSING EMM+P+AF AG  VRVANEL
Sbjct: 272 GIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSINGLEMMVPLAFFAGTSVRVANEL 331

Query: 330 GAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTDVVEAVDSLSNLLAI 389
           GAGNGK A+FA I+SV QS +IGI I ++I     +I  +FSSS  V++AV++LS LL+ 
Sbjct: 332 GAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIGWMFSSSETVLKAVNNLSILLSF 391

Query: 390 TILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWYYKSGVSGIWGGMLF 449
            ILLNS+QPVLSGVAVGSGWQ+ VA+INLGCYY IGLPLG +M W +K GV GIW GM+F
Sbjct: 392 AILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLPLGIVMGWMFKFGVKGIWAGMIF 451

Query: 450 GGSAFQTLILIIITIRTDWEKEAENALKHVEEWS 455
           GG+  QTLILI IT+R DWEKEA+NA   V +WS
Sbjct: 452 GGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of Cp4.1LG11g04550 vs. TAIR 10
Match: AT1G47530.1 (MATE efflux family protein )

HSP 1 Score: 387.1 bits (993), Expect = 2.0e-107
Identity = 210/458 (45.85%), Postives = 291/458 (63.54%), Query Frame = 0

Query: 26  RRSEEVWSK-LWVETQKLWLIVGPTIFARVATFSMNIITQAFAGHLGDVELASISIANTV 85
           + + +VW+K    E+++LW + GP IF  ++ +S+  +TQ F+G LG++ELA++S+ N+V
Sbjct: 22  KSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGALTQTFSGRLGELELAAVSVENSV 81

Query: 86  IVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRSWIMLFLCCFLLLPFYVYATPV 145
           I G  FG++LGM SALETLCGQAYGA +  M+GIY+QRSW++LF     LLP Y++A P+
Sbjct: 82  ISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQRSWVILFTTALFLLPVYIWAPPI 141

Query: 146 LKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQCQHKTLVIAWVSLVGLVVNVV 205
           L   G+A  +++ +G  A+W+IP  F++A  FP+Q FLQ Q K LV+AW+S V LV++ V
Sbjct: 142 LSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFLQSQRKVLVMAWISGVVLVIHAV 201

Query: 206 TSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGWCPLTWTGFSVKAFHGLWEFLK 265
            SWLFI   + G++GAAI L+ SWW++ +G   Y +       WTGFS+ AF  L+ F+K
Sbjct: 202 FSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILITKSDGAWTGFSMLAFRDLYGFVK 261

Query: 266 LSIAAGLML-----------------------------CMSINGWEMMIPVAFLAGVGVR 325
           LS+A+ LML                             CM+I GW  MI + F A + VR
Sbjct: 262 LSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISICMNIEGWTAMISIGFNAAISVR 321

Query: 326 VANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFHGKIALIFSSSTDVVEAVDSLS 385
           V+NELGAGN   AKF+ IV    ST+IGI   +V++        +F+SS  V      ++
Sbjct: 322 VSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLATKDSFPYLFTSSEAVAAETTRIA 381

Query: 386 NLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYGIGLPLGFIMQWYYKSGVSGIW 445
            LL  T+LLNS+QPVLSGVAVG+GWQA VAY+N+ CYY IGLP G ++ +    GV GIW
Sbjct: 382 VLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYYIIGLPAGLVLGFTLDLGVQGIW 441

Query: 446 GGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEW 454
           GGM+  G   QTLILI I   T+W KEAE A   V+ W
Sbjct: 442 GGMV-AGICLQTLILIGIIYFTNWNKEAEQAESRVQRW 478

BLAST of Cp4.1LG11g04550 vs. TAIR 10
Match: AT1G33110.1 (MATE efflux family protein )

HSP 1 Score: 386.7 bits (992), Expect = 2.6e-107
Identity = 206/486 (42.39%), Postives = 304/486 (62.55%), Query Frame = 0

Query: 7   ADAAADLNQALIGRDEDRPRRSEE---VWSKLWVETQKLWLIVGPTIFARVATFSMNIIT 66
           A    +L  AL+ +  +     ++   +  K+W+E++KLW++  P IF R +TF ++II+
Sbjct: 2   AGGGGELTAALLKKTAENGGEEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIIS 61

Query: 67  QAFAGHLGDVELASISIANTVIVGFNFGLLLGMASALETLCGQAYGAKRFHMLGIYLQRS 126
           Q+F GHLG +ELA+ SI  TV++ F+ G+LLGMASALETLCGQAYGAK+ HMLGIYLQRS
Sbjct: 62  QSFIGHLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRS 121

Query: 127 WIMLFLCCFLLLPFYVYATPVLKMLGQADDVAELSGVVAIWLIPLHFSFAFQFPLQVFLQ 186
           WI+L  C   L P Y+++ P+L  LGQ + +  ++ ++A+W+I ++FSF   F  Q+FLQ
Sbjct: 122 WIVLTGCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQ 181

Query: 187 CQHKTLVIAWVSLVGLVVNVVTSWLFIYELQLGVIGAAIALDISWWVLGLGLYFYTVGGW 246
            Q K  +IA+V+ V L V+V  SWL +     G+ GA  +  +++W+  +    +   G 
Sbjct: 182 AQSKNKIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGG 241

Query: 247 CPLTWTGFSVKAFHGLWEFLKLSIAAG-----------------------------LMLC 306
           C  TW GFS+ AF  LW   KLS+++G                             L +C
Sbjct: 242 CKDTWRGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAIC 301

Query: 307 MSINGWEMMIPVAFLAGVGVRVANELGAGNGKAAKFATIVSVAQSTVIGIAICLVIVLFH 366
           ++ING EMMI + FLA   VRV+NELG+GN K AKFAT+ +V  S  +GI +  V +   
Sbjct: 302 LNINGLEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLR 361

Query: 367 GKIALIFSSSTDVVEAVDSLSNLLAITILLNSIQPVLSGVAVGSGWQAWVAYINLGCYYG 426
           G+++ IF++S  V   V  LS LLA +IL+NS+QPVLSGVAVG+GWQ +V Y+NL CYY 
Sbjct: 362 GRVSYIFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYL 421

Query: 427 IGLPLGFIMQWYYKSGVSGIWGGMLFGGSAFQTLILIIITIRTDWEKEAENALKHVEEWS 461
           +G+P+G I+ +     V G+W GMLF G   QT +L ++T+RTDW+++   +L+ +  W 
Sbjct: 422 VGIPIGIILGYVVGLQVKGVWIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRWV 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKQ17.7e-15761.54Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1[more]
Q1PDX91.6e-14658.35Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1[more]
Q9FNC12.6e-14458.37Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1[more]
Q9SX832.8e-10645.85Protein DETOXIFICATION 33 OS=Arabidopsis thaliana OX=3702 GN=DTX33 PE=2 SV=1[more]
Q8W4883.6e-10642.39Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023545612.10.094.13protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo][more]
KAG6598709.10.092.51Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7029651.10.092.11Protein DETOXIFICATION 27 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022962463.10.092.11protein DETOXIFICATION 27-like [Cucurbita moschata] >XP_022962464.1 protein DETO... [more]
XP_022997119.11.55e-31591.30protein DETOXIFICATION 27-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1HEV90.092.11Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111462892 PE=3 SV=1[more]
A0A6J1KAJ67.53e-31691.30Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111492126 PE=3 SV=1[more]
A0A6J1BQ618.44e-26878.10Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004791 PE=3 SV=1[more]
A0A6J1BQC71.45e-25975.92Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004792 PE=3 SV=1[more]
A0A6J1BQ655.83e-25573.85Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111004794 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G65380.15.5e-15861.54MATE efflux family protein [more]
AT5G10420.11.1e-14758.35MATE efflux family protein [more]
AT5G44050.11.8e-14558.37MATE efflux family protein [more]
AT1G47530.12.0e-10745.85MATE efflux family protein [more]
AT1G33110.12.6e-10742.39MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 48..208
e-value: 1.8E-31
score: 109.0
coord: 269..402
e-value: 1.9E-20
score: 73.1
NoneNo IPR availablePANTHERPTHR11206:SF194PROTEIN DETOXIFICATIONcoord: 23..275
NoneNo IPR availablePANTHERPTHR11206:SF194PROTEIN DETOXIFICATIONcoord: 273..451
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 273..451
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 23..275
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 38..445
e-value: 4.65204E-133
score: 389.239

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g04550.1Cp4.1LG11g04550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity