Cp4.1LG11g02720 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g02720
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	DNA-directed RNA polymerase subunit
Location	Cp4.1LG11: 1432673 .. 1435769 (-)
RNA-Seq Expression	Cp4.1LG11g02720
Synteny	Cp4.1LG11g02720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGACTGAAATTGGTTGCGATTATTTTGATTGGGAGTCTGGGAGTATGGTTCGTATTTTCGCGCGGTTCGGTAGGTCTCCACACATTCGGTTAGGTCCCAGAGATGAATGGGCGAACCCAACGGAACCAATTTCCAGTGCAATTTCCGGAGGGTTTAAGCTTCCAAAACCATATTGTTTTCGTTCTGCTTTAGCAAGTTTTACTTTAAGGTCGATTGTTTATCCATATGTCCGCCCAGCTCAGCTATCTTTGGCTATCCACCGTTTTCTTCCACTGGTAGCTCGCCCCTTTTCGACAATAAATCTGGGGAAGCTCTTTCTTGGGCTTGATTAAATTGTGCTTACTAATTCTTATGAGGACCCCCCTTGTGGTTTCAGCTGCAATTTTTCTTTTTATATTCTTTTTCGTTTGGAGTTTTAGCCTTGTTTTGGTCTCTTATTTGTTGTAATTGAACTTCTTATAATTGTTCTAACTATTTTTTTTATGAACACTGTTTGTCAGAAGCGCTTGGGGAAGCAGCAAGGCCATTTATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAACTTGTGTGGAACCATGTTGTCATTCAGCTCAACGAAATATGTCGAGTGTCCTTTGTGCAAGTCTAGACGAAGTGCAAAAGGTGAACATACACATCGGAGTTAATCTTATTCACTTTTAGTTTTTCATTTTGGGTAATTTAAACATTCGAACACACATTACTTATATGAATGAGTTCTTAATTTGTAGTCTAGTTCATTTTTTCTTCATGTTTATGTAGCTAAAAATGTGAAAAACATGATTTATTTTTTCATTTTCTTTCTCAAGTTTCTATTTTCCTTTTTAATCTTTTTACTAGAACTGAGTTGTGCAGGTTGGTTTTTCTTTAATCTATCAACTTAAATTTAAGATGATTTTGGTAGTATAATTTTTATTCCGCTCACAAACGTTTATCAAGAATTTTATAAGGGCCGATAGTTAAGGAGGATTATGGGTCCTATTGTCTTACACCTCCTTGGCTTCGTTCTCCTTTGCCATAAAGGTTATAAGTCTCGGTTATATTCACACCAAGAATTCCTTTCTTCGAGAAGCCTAGATTGTTGACATGGTATCATGCTCGAGAGAGAGAATATCCCAAGTTTTCGTGAAGAACTATGGAAAAACCATCTGGACCCGTAGCTTGGCCTTGACTAAGACTAGAAATAGCCCTTGGAACTTTATGCTAAATAAAAGCCTGCGGCATATGTTATTCGGCTGTAGTGATAGAATTCCAACACAGACCGTGGAAGATTGTGTGGTCCTCTCAGAAAAACTTAGTGTGGTAGACTGCTGGGGTTCTGACAATTGGATCCCTCAAATTCATAAGGAAGCTGACTGGTGTAGGTGAATGAATAGGCTGAATTGATCATGATTTTGAAAGCTTGTTCTTCAAACTATCAGATGGACCTTCGAAATTTTGGTTTGATTTCAAACCAAATGTAGTAATAGACTAATAACAATGAAGGCTGCGTGTGAAAAATGTTTCTTTAGATTTGGCAGATGGAACCTCTGCCTTCTCATACCTGAGCTAGCTTACAATTACATGAAGAAATTGTAATTACAATTACCTGAGTTATCTGAGAGTTCTTTCAAATGAAATCAATGATTTCTTGACCACAATTACATGAAGAAAGTGAGATAGCTATAACAATGTCTGTGGAACCAAGTACTAAGTACTAACAGCTAACCAGAGACTGAAAATTATAGAACTCATTACAATTCTCAGGCTGGTTAGTCTTATGCAAAGGCCCAAGTGCCATCATGATTTCCCTGAATATTATTCTATGCATCTTGAAATTGGTTTTCTGAAGGATATATATATCAAATCATTTTAGAATTTGTGGGCTTTGCTTTATTTTTAATCGTTTTGTTGAAAGAACTATGCATCTTATCATCTTTGCATATTGAAATTGAAAGTATTGGAGTCCCAATCTTTCTTTTCATGATTTGAGGCATCGAAGTTGTGCAAATGCAGTTGAGAAGCTTTTCCTTAAATATTTCATGCACTCTTTTAGTAAATATCTCTAACCTGCGTGTTGTGGGCTTATTTCAGAGATCGTCGGCAGAGAAATAAGTTACACAGTTACTGCTGAGGTATGCCTTTTGAAGCATAAACATTATCATCCCCATGCTTGTTCTATTTCTAGCTCTAAGACATCCCAGCAACATGTCATTCCTATGCTTTTGAACTCTAGGATACTCGAAAATTTGCTAAGATTGTTTCTGGATGAATTTACCTATATGATGATTCTCTACGCTCGGCAAATCAATAAGCCTACCTAACCCCCAAAATTCATATTGGGTCGATATCCTCTAGCGGTGAAAATTTTTAGTATAAGCAAAAGTTCATATGATCTTGATAAGACCCCTTCTATTTGATTGCCAGACATCCAAGAAATGATGTAGTTTTTGAAATCTTCGTGTGAACGATTTGCAGGAAATTAGAAAGCAGTTGGGAATTTCATTGATTGATGAAGAGAAAATGCAGCTATCAAAGGTAGTAGGAGAAAAGAAGTGTATGGTATAAGTATTCTGCTTGAAACGTGTAAGTAGTAGGCACTAATATGAAGCTGCCATGTTTGACAGGAAAGGAGGAGATGTGAGAAATGTGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGGTACTTTTCATACCAACTCATAAGCCCACGCCCTTTAGGCTTTCTCTTTTATTCCCATTAGAAAGAGTCCCAAGTTTGAGTGTGGTTTCAATAAAAATGCAGATGAGATCGGCTGATGAAGGGCAGACCACCTTTTATACGTGCACCAAGTGCCATCATCAGACCAGAGAGAATTGATCCTCCACATAACATTATAAAATATGTATGTTTCACCATAGTTCGTCTTTTATTCTTTAGGGAGCAGCAAAGTCCTTGAGTTAGGATAGCTCTTAACGCATTCCAAAAGCTTAAACGTGGCGAATTTTGTTCATTACGACGATCTCGCTCTAGATGAAGTATAATTTTATTTTTAGTTTTCTGTTTACCAAGTATGCTTAATCTTTTTTTTTTTTTTTTTTGGGNTTTTC

mRNA sequence

ATGTCGACTGAAATTGGTTGCGATTATTTTGATTGGGAGTCTGGGAGTATGGTTCGTATTTTCGCGCGGTTCGAAGCGCTTGGGGAAGCAGCAAGGCCATTTATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAACTTGTGTGGAACCATGTTGTCATTCAGCTCAACGAAATATGTCGAGTGTCCTTTGTGCAAGTCTAGACGAAGTGCAAAAGAGATCGTCGGCAGAGAAATAAGTTACACAGTTACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTGATTGATGAAGAGAAAATGCAGCTATCAAAGGAAAGGAGGAGATGTGAGAAATGTGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGGCAGACCACCTTTTATACGTGCACCAAGTGCCATCATCAGACCAGAGAGAATTGATCCTCCACATAACATTATAAAATATGTATGTTTCACCATAGTTCGTCTTTTATTCTTTAGGGAGCAGCAAAGTCCTTGAGTTAGGATAGCTCTTAACGCATTCCAAAAGCTTAAACGTGGCGAATTTTGTTCATTACGACGATCTCGCTCTAGATGAAGTATAATTTTATTTTTAGTTTTCTGTTTACCAAGTATGCTTAATCTTTTTTTTTTTTTTTTTTGGGNTTTTC

Coding sequence (CDS)

ATGTCGACTGAAATTGGTTGCGATTATTTTGATTGGGAGTCTGGGAGTATGGTTCGTATTTTCGCGCGGTTCGAAGCGCTTGGGGAAGCAGCAAGGCCATTTATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAACTTGTGTGGAACCATGTTGTCATTCAGCTCAACGAAATATGTCGAGTGTCCTTTGTGCAAGTCTAGACGAAGTGCAAAAGAGATCGTCGGCAGAGAAATAAGTTACACAGTTACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTGATTGATGAAGAGAAAATGCAGCTATCAAAGGAAAGGAGGAGATGTGAGAAATGTGGGAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGGCAGACCACCTTTTATACGTGCACCAAGTGCCATCATCAGACCAGAGAGAATTGA

Protein sequence

MSTEIGCDYFDWESGSMVRIFARFEALGEAARPFMAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN

Homology

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy Swiss-Prot
Match: Q5TM50 (DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta OX=9544 GN=POLR1H PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-11
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 0


Query: 42  DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 101
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCTRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 102 EKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 151
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy Swiss-Prot
Match: Q1RMP0 (DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus OX=9913 GN=POLR1H PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.1e-11
Identity = 37/110 (33.64%), Postives = 60/110 (54.55%), Query Frame = 0


Query: 42  DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 101
           D  FC  CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 13  DLDFCPDCGSVLPLPGVQDAVACTRCGFSINVRDFEGKVVKTSVVFNKLGTAMPLSMEEG 72

Query: 102 EKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 151
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 73  PEFQGPVVDRRCSRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 122

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy Swiss-Prot
Match: Q9P1U0 (DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens OX=9606 GN=POLR1H PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.2e-11
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 0


Query: 42  DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 101
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 102 EKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 151
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy Swiss-Prot
Match: Q1XHV8 (DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes OX=9598 GN=POLR1H PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.2e-11
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 0


Query: 42  DFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 101
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 102 EKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 151
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy Swiss-Prot
Match: Q6MFY5 (DNA-directed RNA polymerase I subunit RPA12 OS=Rattus norvegicus OX=10116 GN=Polr1h PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-10
Identity = 41/123 (33.33%), Postives = 64/123 (52.03%), Query Frame = 0


Query: 29  EAARPFMAYSRGHDFLFCNLCGTMLSFSSTK-YVECPLCKSRRSAKEIVGREISYTVTAE 88
           E ARP   +    D  FC  CG++L     +  V CP C      ++  G+ +  +V   
Sbjct: 2   ELARPCSNFQ--SDLDFCPDCGSVLPLPGVQDTVICPRCGFSIDVRDFGGKVVKTSVVFN 61

Query: 89  EIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQT 148
           ++   + +S+ +  + Q     RRC +CG++   + +RQMRSADEGQT FYTC  C  Q 
Sbjct: 62  KLGTVIPMSVDEGPESQGPVVDRRCSRCGHEGMAYYTRQMRSADEGQTVFYTCINCKFQE 121

Query: 149 REN 151
           +E+
Sbjct: 122 KED 122

[Close]

BLAST of Cp4.1LG11g02720 vs. NCBI nr
Match: KAG6598510.1 (DNA-directed RNA polymerase I subunit RPA12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 257 bits (657), Expect = 9.51e-86
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 25  EALGEAARPFMAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTV 84
           EALGEAARPFMAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTV
Sbjct: 21  EALGEAARPFMAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTV 80

Query: 85  TAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCH 144
           TAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCH
Sbjct: 81  TAEEIRKQLGISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCH 140

Query: 145 HQTREN 150
           HQTREN
Sbjct: 141 HQTREN 146

[Close]

BLAST of Cp4.1LG11g02720 vs. NCBI nr
Match: XP_023546814.1 (DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita pepo subsp. pepo] >KAG7029447.1 DNA-directed RNA polymerase I subunit RPA12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 238 bits (606), Expect = 2.09e-78
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. NCBI nr
Match: XP_022144336.1 (DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia] >XP_022144337.1 DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia])

HSP 1 Score: 236 bits (601), Expect = 1.21e-77
Identity = 114/116 (98.28%), Postives = 116/116 (100.00%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEI+GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. NCBI nr
Match: XP_022962626.1 (DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita moschata] >XP_022962627.1 DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita moschata])

HSP 1 Score: 235 bits (599), Expect = 2.44e-77
Identity = 115/116 (99.14%), Postives = 115/116 (99.14%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYS GHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSGGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. NCBI nr
Match: XP_022997634.1 (DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima] >XP_022997635.1 DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima])

HSP 1 Score: 234 bits (598), Expect = 3.46e-77
Identity = 114/116 (98.28%), Postives = 115/116 (99.14%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK I+GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKGIIGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy TrEMBL
Match: A0A6J1CTE6 (DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC111014043 PE=3 SV=1)

HSP 1 Score: 236 bits (601), Expect = 5.85e-78
Identity = 114/116 (98.28%), Postives = 116/116 (100.00%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEI+GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIIGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEK+QLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKVQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy TrEMBL
Match: A0A6J1HDS4 (DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC111463020 PE=3 SV=1)

HSP 1 Score: 235 bits (599), Expect = 1.18e-77
Identity = 115/116 (99.14%), Postives = 115/116 (99.14%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYS GHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSGGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy TrEMBL
Match: A0A6J1KC19 (DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111492509 PE=3 SV=1)

HSP 1 Score: 234 bits (598), Expect = 1.68e-77
Identity = 114/116 (98.28%), Postives = 115/116 (99.14%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAK I+GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKGIIGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy TrEMBL
Match: A0A0A0LKC4 (DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_2G345940 PE=3 SV=1)

HSP 1 Score: 232 bits (592), Expect = 1.38e-76
Identity = 113/116 (97.41%), Postives = 114/116 (98.28%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYSRGHDFLFCNLCGTMLSF STKYVECP CKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLIDEEKMQL+KERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. ExPASy TrEMBL
Match: A0A1S3BBZ4 (DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103488399 PE=3 SV=1)

HSP 1 Score: 228 bits (581), Expect = 6.55e-75
Identity = 111/116 (95.69%), Postives = 113/116 (97.41%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           MAYSRGHDFLFCNLCGTMLSF STKYVECPLCKS+RSAKEI GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSKRSAKEINGREISYTVTAEEIRKQLG 60

Query: 95  ISLIDEEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 150
           ISLI EEKMQL+KERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIGEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of Cp4.1LG11g02720 vs. TAIR 10
Match: AT3G25940.1 (TFIIB zinc-binding protein )

HSP 1 Score: 133.3 bits (334), Expect = 1.7e-31
Identity = 66/118 (55.93%), Postives = 87/118 (73.73%), Query Frame = 0


Query: 35  MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
           M  SR  +FLFCNLCGTML   STKY ECP CK+ R+AK+I+ +EI+YTV+AE+IR++LG
Sbjct: 1   MEKSRESEFLFCNLCGTMLVLKSTKYAECPHCKTTRNAKDIIDKEIAYTVSAEDIRRELG 60

Query: 95  ISLIDEE---KMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 150
           ISL  E+   + +L K ++ CEKC + E  + +RQ RSADEGQTT+YTC  C H+  E
Sbjct: 61  ISLFGEKTQAEAELPKIKKACEKCQHPELVYTTRQTRSADEGQTTYYTCPNCAHRFTE 118

[Close]

BLAST of Cp4.1LG11g02720 vs. TAIR 10
Match: AT1G20065.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: TFIIB zinc-binding protein (TAIR:AT3G25940.1). )

HSP 1 Score: 85.1 bits (209), Expect = 5.2e-17
Identity = 38/62 (61.29%), Postives = 48/62 (77.42%), Query Frame = 0


Query: 35 MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 94
          M  SR  D LFCNLCGTML   STKY ECPLC++ R+ KEI+ + ++YTVT E+IR++LG
Sbjct: 1  MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGKEIIDKNLAYTVTTEDIRRELG 60

Query: 95 IS 97
          IS
Sbjct: 61 IS 62

[Close]

BLAST of Cp4.1LG11g02720 vs. TAIR 10
Match: AT1G20065.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: TFIIB zinc-binding protein (TAIR:AT3G25940.1). )

HSP 1 Score: 73.2 bits (178), Expect = 2.0e-13
Identity = 32/53 (60.38%), Postives = 39/53 (73.58%), Query Frame = 0


Query: 35 MAYSRGHDFLFCNLCGTMLSFSSTKYVECPLCKSRRSAKEIVGREISYTVTAE 88
          M  SR  D LFCNLCGTML   STKY ECPLC++ R+ KEI+ + ++YTVT E
Sbjct: 1  MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGKEIIDKNLAYTVTTE 53

[Close]

BLAST of Cp4.1LG11g02720 vs. TAIR 10
Match: AT4G07950.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 50.1 bits (118), Expect = 1.8e-06
Identity = 31/110 (28.18%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 45  FCNLCGTMLSF---SSTKYV--ECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLID 104
           FC  CG +L +    S+++    CP   +     EI  +++        ++K +   +  
Sbjct: 3   FCPTCGNLLRYEGGGSSRFFCSTCPYVANIERRVEIKKKQLL-------VKKSIEPVVTK 62

Query: 105 EEKMQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 150
           ++    ++    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  DDIPTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

[Close]

BLAST of Cp4.1LG11g02720 vs. TAIR 10
Match: AT1G01210.1 (DNA-directed RNA polymerase, subunit M, archaeal )

HSP 1 Score: 48.9 bits (115), Expect = 4.1e-06
Identity = 29/107 (27.10%), Postives = 50/107 (46.73%), Query Frame = 0


Query: 45  FCNLCGTMLSF--SSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDEEK 104
           FC  CG +L +         C  C         + R++        ++K +   +  ++ 
Sbjct: 3   FCPTCGNLLRYEGGGNSRFFCSTC----PYVAYIQRQVEIKKKQLLVKKSIEAVVTKDDI 62

Query: 105 MQLSKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 150
              ++    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  PTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g02720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5TM50	5.4e-11	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta OX=9544 GN=POLR1H ...	[more]
Q1RMP0	7.1e-11	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus OX=9913 GN=POLR1H PE=2...	[more]
Q9P1U0	9.2e-11	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens OX=9606 GN=POLR1H PE...	[more]
Q1XHV8	9.2e-11	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes OX=9598 GN=POLR1H...	[more]
Q6MFY5	1.6e-10	33.33	DNA-directed RNA polymerase I subunit RPA12 OS=Rattus norvegicus OX=10116 GN=Pol...	[more]

BLAST of Cp4.1LG11g02720 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6598510.1	9.51e-86	100.00	DNA-directed RNA polymerase I subunit RPA12, partial [Cucurbita argyrosperma sub...	[more]
XP_023546814.1	2.09e-78	100.00	DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita pepo subsp. pepo] >K...	[more]
XP_022144336.1	1.21e-77	98.28	DNA-directed RNA polymerase I subunit RPA12-like [Momordica charantia] >XP_02214...	[more]
XP_022962626.1	2.44e-77	99.14	DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita moschata] >XP_022962...	[more]
XP_022997634.1	3.46e-77	98.28	DNA-directed RNA polymerase I subunit RPA12-like [Cucurbita maxima] >XP_02299763...	[more]

BLAST of Cp4.1LG11g02720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTE6	5.85e-78	98.28	DNA-directed RNA polymerase subunit OS=Momordica charantia OX=3673 GN=LOC1110140...	[more]
A0A6J1HDS4	1.18e-77	99.14	DNA-directed RNA polymerase subunit OS=Cucurbita moschata OX=3662 GN=LOC11146302...	[more]
A0A6J1KC19	1.68e-77	98.28	DNA-directed RNA polymerase subunit OS=Cucurbita maxima OX=3661 GN=LOC111492509 ...	[more]
A0A0A0LKC4	1.38e-76	97.41	DNA-directed RNA polymerase subunit OS=Cucumis sativus OX=3659 GN=Csa_2G345940 P...	[more]
A0A1S3BBZ4	6.55e-75	95.69	DNA-directed RNA polymerase subunit OS=Cucumis melo OX=3656 GN=LOC103488399 PE=3...	[more]

BLAST of Cp4.1LG11g02720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25940.1	1.7e-31	55.93	TFIIB zinc-binding protein	[more]
AT1G20065.1	5.2e-17	61.29	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT1G20065.2	2.0e-13	60.38	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT4G07950.1	1.8e-06	28.18	DNA-directed RNA polymerase, subunit M, archaeal	[more]
AT1G01210.1	4.1e-06	27.10	DNA-directed RNA polymerase, subunit M, archaeal	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001222	Zinc finger, TFIIS-type	SMART	SM00440	Cys4_2	coord: 110..149 e-value: 3.7E-12 score: 56.4
IPR001222	Zinc finger, TFIIS-type	PFAM	PF01096	TFIIS_C	coord: 111..146 e-value: 1.4E-15 score: 56.8
IPR001222	Zinc finger, TFIIS-type	PROSITE	PS51133	ZF_TFIIS_2	coord: 108..148 score: 12.826397
None	No IPR available	GENE3D	2.20.25.10		coord: 96..149 e-value: 3.6E-16 score: 60.4
None	No IPR available	PANTHER	PTHR11239:SF19	DNA-DIRECTED RNA POLYMERASE SUBUNIT	coord: 35..148
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 89..150
IPR012164	DNA-directed RNA polymerase subunit/transcription factor S	PANTHER	PTHR11239	DNA-DIRECTED RNA POLYMERASE	coord: 35..148
IPR034004	Pol I subunit A12, C-terminal zinc ribbon	CDD	cd10507	Zn-ribbon_RPA12	coord: 110..150 e-value: 3.4888E-21 score: 78.784

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g02720.1	Cp4.1LG11g02720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006379	mRNA cleavage
biological_process	GO:0006363	termination of RNA polymerase I transcription
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding