Cp4.1LG11g02250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g02250
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Cysteine protease
Location	Cp4.1LG11: 1234365 .. 1237861 (+)
RNA-Seq Expression	Cp4.1LG11g02250
Synteny	Cp4.1LG11g02250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCATAATATCTCAGATCCCCGTTCACTCTGTCACTGTCTCTGTGGAGCGGCTCGCTGCAATCGCTTTCTTCTTTACCATTCTACTTTCGGCGACGGTAGCCTACGACGTATCTTCCGATCTAATGTACGGCGGCGACAGCGAGGACGAAGATCTTGTGATCCTTGAAGTCGTCTCCTTACCGCTTAGCAAAAAACTTCCAGAACTCCGAACTCAAGTTCGGAAAGGCCTACGCCATTGATGAGGAGCACGATTACAGGTTCCGTGTATTCAAGGCTTACCTCCGTAGAGCCAAGCACCAGCAGGAGCTCGATCCTGGCGCCGTCCATGGCGTCACGTGGTTATCCGATCTCACCGAGTCCTAGTTCCGTACACATTTCGTCGGAGTGAATCGGCTCCGTCTTTCTGCCGATTCTCAGAAGGTTCCGATCCTTCCTATTGGCGACCTAGCCTCCTACTTCGATTGACGTTACCACGGTTCCGTCCCGCTGGTCAAAGAGCAGGTATGAGTTATTTCGGAAAGTTTTCTCAAGTCTGTCGAGTATTTGAACTTCCGATTTTGCTTTCTTATTTTTTGAGAGAATTGTCTGTGTTCTGTATCGTTTAGGATTCACGTGGCTTGCGCTGGTCTTTGAGTGAGTAACAGCTTGTTGATTCATGAGGTTCGTTCCATTCCTTTCCTGCCTTGGATTTATTGCCTTTTGTTTCTATTTGCAATTGTTACTACGATCCTCTTTCTATCTCCTTAGCCTGCTGCTGCAAAAGTCGAACCAAATAAAATAAATTGAGGTTCGGTTTATAACCTGTTGTTTTTAGAAACTGTGCTGGTTTACTCCTACTTTTTGTCTTAAAATCTACTGTATGTTTTTTCTTTCTTTCCTTTATTGTGATAGGAAACAGAATAGTTCTTATGTGAAAGTTTAAAATCATTTTAAAATATGACTTTATTAATTCAAAATCAATTTTATGTTTGATTATTTTTCATACATGATCATAATTGATGTTCAATAGCATGATTGGAAATAAGTTTATATATAAAAATAGTTTTAACTAGTTAAAATTTACTTTAGAGTAGGTTTCTAGTCTCTTTTTTAAAATTTGATTAGATTTCGAAACTTCAAAAAAAATTAAGAAAAACAGTTGTAAGTTTGATGTGTTTTTTTAATGGTCAGAACAGTATAATGATCTGTATATATCTTTTTCAAAGTCAATGAAATGATATATCCATGAACAAATCTGTTTTTTCTCTCTAAGGACGGCATGAACCTATATCTATTCAGGTCTTTTCTTTTCTTTTTTCAACGAGAATCTATTCAGGTCATGTTGAAAAACTGTTGTTGCTTTGCGAAACTAAGGAATGATTTCGTACTCTCTGTTTCCGTTTATTTCAAGCGGAAATGTTAGTAGAATTCATTTATTTATTAAAGTTAAAAATAATTCTTAGAGGAAAATTTTTATTCCCTACTTTATGAACTATATATCATTTTATGTAACAAATGGGAAACACATCGACGTTTTTGGTAATTTGGAGTCGGCGACAAATGAATAATTTAGTATTGTTCTTATCTTTTCCAAAAGAGAAGAATAATTATTTTTGAAGTTAGGAAATTCTGAAGAAATATATAAAGACATCACAAGATTTTGTTTTATGTTAGTTGTTGAAGAGATTACAAGGAAGTTATCTTTGAAATGTTATTTGAAAATTAATATATTATTTTTGTTTATGATAAAATTATAGAGAATTTATTGGGTGATTTTTGACCCTGACGCTTTTTGGTTGTTTCCTATTTTCTAAATATAATCTTGAAATTTTGACTAAAATTTAAACGTTTAATCTGTTCTACAAAGTTATTACTTTCAACATTTGAAATTCAATTAAAATTTTAAAAATACTCTTATAGTTTAATTAGTTGAACTAAAAATAGGAATAGAATTAAATTATTTATTTTGTTGTCCATGCTGAAACCACAAATCCTATTAAAGCTACCCAAACTTTATTGAATTGCAAGAAAAAGATGAAGTTACTTCGAAATATAGTGTTACTGTTGTTGAAATGAATTAAACTGACAAATATTTGAACAGATGTTTTAGTTCAAATTAGTTGGTCTAAACGAATTCAACATGAATCAATCAATAGTNAAGAGTTTTTTTTTTTTTTTTTTTTTACGGGGTTGTCATTTAGATGGGTTATATCAAAACACTCCATTATTTATGTAGTTGAGTTCTAACTCCCACGTTGATCGAGTAGTTAGAAATTTCTACCACTAGCACTCACCACTATATTTTTTACAAATTGTTATATTTCTTTATTTATATATTTTTGGGAGAAGTCTCCTCTCCCCCTCCACTAATCTTCTTACAATATTATTATTATTATTATTGTTTTGGAAAATACTCTTTCTGCCCTCTCTCCACTAAAATTTTGACAATTTTTTTTTGTTGGGAAACAAATTTTTGTATGTAGTTGTTTGGATAGTATGGTTCTTGGACCTTGAGATGGGAACACGAATATCAAAACGTTAAGTTCATCACATGCATTTCTGACCACCAAATTTTAGCATACGATCCCTTAAAAGATAACGAAAACCATTGAAATAAGATTTTATGATATTTTTTTATGTTGTAGAAATTAAGCTGATTTATAACATAGTACGGTAGATATGGTATCGAATACCTTTTCAAAGTTGGAAGTTTAATTCCTACGTTATTGTACAAAAGAAAAGAAAAGGGAAAGGGTCATTTCCATTTTGATTAAAAAATAAGTTTTTAACAACACATTTTGATGATGGCTATTATATTGCTTGAACTTAGAACTCATCAATTAATAATCGACCTCCAGATATTCATGACATTATCAGTAACCTCTCTGAGTAATTTCTCGTTAACCATAGTTAAGATGACAGTATCAGTGTCATCAGTGAAAATTTGAAAGTAAAAAAGTAGACTTTTGCAGTGTGATCCTGAGGAAGCTGGTGCATGCGACGCGATGGGGGACTGATGAACTCATCTTTTGAGTACTTTTTTTAAAGCTGGTGGATTGGAGCGACAGGAGGACTATCCTTCCACTGGGAGGTATCGTGGCCCTTGCAAATTCCAAAGCGGCATAAAGATGCCGACCAAAGTGCAGCAAACTTGATCAAGAATGGCCCTTCAGCTTAGTAATGAAATTCTTCTCCATTATTATCATCAATTTTTAAATGCGTTATTTGATTTCTCAACCTGCTTTAAACCTCATTGGTGATGTGAAAAACTGAAGCTAATGATTTGGGCCTTTTCTTTAACGTAGACATTGTTTGTTAACCATTTCCTTTTTAGTTTTCTACTTTATCTTAGACCGCTGCTGTTGGAGTCGATGCAATTTTCCTGCAGACCAACGTAAGAGGAATTTCATGCGTGGGTGAAAACTGGGGAGAAAATGGCTACTACTTAATCTGCAAGGGCAACAATACCTGTGGATCTGAGTCGATGGTCTCGTCTGTGGCTGCTACCCCAAACTAA

mRNA sequence

CCCATAATATCTCAGATCCCCGTTCACTCTGTCACTGTCTCTGTGGAGCGGCTCGCTGCAATCGCTTTCTTCTTTACCATTCTACTTTCGGCGACGGTAGCCTACGACGTATCTTCCGATCTAATGTACGGCGGCGACAGCGAGGACGAAGATCTTTCGTCTCCTTACCGCTTAGCAAAAAACTTCCAGAACTCCGAACTCAAGTTCGGAAAGGCCTACGCCATTGATGAGGAGCACGATTACAGGTTCCGTAGCCAAGCACCAGCAGGAGCTCGATCCTGGCGCCGTCCATGGCGTCACGTGGTTATCCGATCTCACCGAGTCCTAGTTCCGTACACATTTCGTCGGAGTGAATCGGCTCCGTCTTTCTGCCGATTCTCAGAAGGTTCCGATCCTTCCTATTGGCGACCTAGCCTCCTACTTCGATTGACGTTACCACGGTTCCGTCCCGCTGGTCAAAGAGCAGCTGGTGGATTGGAGCGACAGGAGGACTATCCTTCCACTGGGAGGTATCGTGGCCCTTGCAAATTCCAAAGCGGCATAAAGATGCCGACCAAAACCGCTGCTGTTGGAGTCGATGCAATTTTCCTGCAGACCAACGTAAGAGGAATTTCATGCGTGGGTGAAAACTGGGGAGAAAATGGCTACTACTTAATCTGCAAGGGCAACAATACCTGTGGATCTGAGTCGATGGTCTCGTCTGTGGCTGCTACCCCAAACTAA

Coding sequence (CDS)

CCCATAATATCTCAGATCCCCGTTCACTCTGTCACTGTCTCTGTGGAGCGGCTCGCTGCAATCGCTTTCTTCTTTACCATTCTACTTTCGGCGACGGTAGCCTACGACGTATCTTCCGATCTAATGTACGGCGGCGACAGCGAGGACGAAGATCTTTCGTCTCCTTACCGCTTAGCAAAAAACTTCCAGAACTCCGAACTCAAGTTCGGAAAGGCCTACGCCATTGATGAGGAGCACGATTACAGGTTCCGTAGCCAAGCACCAGCAGGAGCTCGATCCTGGCGCCGTCCATGGCGTCACGTGGTTATCCGATCTCACCGAGTCCTAGTTCCGTACACATTTCGTCGGAGTGAATCGGCTCCGTCTTTCTGCCGATTCTCAGAAGGTTCCGATCCTTCCTATTGGCGACCTAGCCTCCTACTTCGATTGACGTTACCACGGTTCCGTCCCGCTGGTCAAAGAGCAGCTGGTGGATTGGAGCGACAGGAGGACTATCCTTCCACTGGGAGGTATCGTGGCCCTTGCAAATTCCAAAGCGGCATAAAGATGCCGACCAAAACCGCTGCTGTTGGAGTCGATGCAATTTTCCTGCAGACCAACGTAAGAGGAATTTCATGCGTGGGTGAAAACTGGGGAGAAAATGGCTACTACTTAATCTGCAAGGGCAACAATACCTGTGGATCTGAGTCGATGGTCTCGTCTGTGGCTGCTACCCCAAACTAA

Protein sequence

PIISQIPVHSVTVSVERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDLSSPYRLAKNFQNSELKFGKAYAIDEEHDYRFRSQAPAGARSWRRPWRHVVIRSHRVLVPYTFRRSESAPSFCRFSEGSDPSYWRPSLLLRLTLPRFRPAGQRAAGGLERQEDYPSTGRYRGPCKFQSGIKMPTKTAAVGVDAIFLQTNVRGISCVGENWGENGYYLICKGNNTCGSESMVSSVAATPN

Homology

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 3.6e-09
Identity = 42/141 (29.79%), Postives = 57/141 (40.43%), Query Frame = 0


Query: 157 GGLERQEDYPSTGRYRGPCKFQ---------------------SGIKMPTKTAAVGVDAI 216
           GGL R++DYP TG   G CK                       +   +     AV ++A 
Sbjct: 219 GGLMREKDYPYTGTDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAA 278

Query: 217 FLQTNVRGISCV--------------------------------------GENWGENGYY 239
           ++QT + G+SC                                       GE+WGENG+Y
Sbjct: 279 YMQTYIGGVSCPYICSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFY 338

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy Swiss-Prot
Match: Q8VYS0 (Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.8e-09
Identity = 45/146 (30.82%), Postives = 59/146 (40.41%), Query Frame = 0


Query: 156 AGGLERQEDYPSTGRYRGPCKFQSGIKMPTKTA--------------------------A 215
           AGGLE +  YP TG+ RG CKF      P K A                          A
Sbjct: 223 AGGLEEERSYPYTGK-RGHCKFD-----PEKVAVRVLNFTTIPLDENQIAANLVRHGPLA 282

Query: 216 VGVDAIFLQTNVRGISCV---------------------------------------GEN 237
           VG++A+F+QT + G+SC                                        G+ 
Sbjct: 283 VGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKK 342

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 8.1e-09
Identity = 45/144 (31.25%), Postives = 60/144 (41.67%), Query Frame = 0


Query: 156 AGGLERQEDYPSTGRYRGPCKFQ---------------------SGIKMPTKTAAVGVDA 215
           AGGLE ++DYP TG   G CKF                      S   +     A+G++A
Sbjct: 223 AGGLESEKDYPYTGS-DGKCKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINA 282

Query: 216 IFLQTNVRGISCV--------------------------------------GENWGENGY 238
            ++QT + G+SC                                       GENWGENGY
Sbjct: 283 AYMQTYIGGVSCPYICGRHLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGY 342

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-08
Identity = 42/141 (29.79%), Postives = 57/141 (40.43%), Query Frame = 0


Query: 157 GGLERQEDYPSTGRYRGPCKFQSGIKMPTKT---------------------AAVGVDAI 216
           GGL ++EDYP TG+    CK      + + +                      AV ++A 
Sbjct: 222 GGLMKEEDYPYTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAG 281

Query: 217 FLQTNVRGISCV--------------------------------------GENWGENGYY 239
           ++QT + G+SC                                       GE WGENG+Y
Sbjct: 282 YMQTYIGGVSCPYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFY 341

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.6e-07
Identity = 40/142 (28.17%), Postives = 59/142 (41.55%), Query Frame = 0


Query: 156 AGGLERQEDYPSTGRYRGPCKFQSGIKMPTKT---------------------AAVGVDA 215
           AGGL ++EDYP TGR    CKF     + + +                      A+ ++A
Sbjct: 226 AGGLMKEEDYPYTGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINA 285

Query: 216 IFLQTNVRGISCV--------------------------------------GENWGENGY 238
           +++QT + G+SC                                       G  WGE+GY
Sbjct: 286 MWMQTYIGGVSCPYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGY 345

[Close]

BLAST of Cp4.1LG11g02250 vs. NCBI nr
Match: XP_038884968.1 (cysteine protease RD19A-like [Benincasa hispida])

HSP 1 Score: 141 bits (355), Expect = 5.87e-36
Identity = 123/372 (33.06%), Postives = 156/372 (41.94%), Query Frame = 0


Query: 16  ERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL--------SSPYRLA--KNFQNS 75
           +RLA  A FFTILLSATV Y VSSD + G  S+DEDL        +   RL   ++FQN 
Sbjct: 2   DRLAVFALFFTILLSATVTYGVSSDQINGAQSDDEDLLIRQVVSGADDRRLTAEQHFQNF 61

Query: 76  ELKFGKAYAIDEEHDYRFRSQAPAGARSWRRPWR------HVVIRS-------------- 135
           +LKFGK YA DEEHDYRFR    A  R  +R  +      H V R               
Sbjct: 62  KLKFGKTYATDEEHDYRFRI-FKANLRKAKRHQKLDPDAVHGVTRFSDLTESEFRTNFVG 121

Query: 136 -HRVLVPYTFRRS------ESAPSFCRFSEG---------SDPSYWRPSLLLRLTLPRFR 195
            +R+ +P   +++      + AP F    +G         S  S W  S +  +    F 
Sbjct: 122 LNRLRLPADAQKAPILPTDDLAPDFDWRDQGAVTPVKDQGSCGSCWSFSAVGAVEGANFL 181

Query: 196 PAGQRA----------------------------------------AGGLERQEDYPSTG 240
             G+                                          AGGLE++EDYP TG
Sbjct: 182 STGKLVSLSEQQLVDCDHECDPEEAGACDAGCGGGLMNSAFQYILKAGGLEKEEDYPYTG 241

[Close]

BLAST of Cp4.1LG11g02250 vs. NCBI nr
Match: XP_004152668.1 (cysteine protease RD19A [Cucumis sativus] >KGN62647.1 hypothetical protein Csa_021896 [Cucumis sativus])

HSP 1 Score: 129 bits (323), Expect = 2.75e-31
Identity = 115/371 (31.00%), Postives = 145/371 (39.08%), Query Frame = 0


Query: 16  ERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL----------SSPYRLAKNFQNS 75
           ER  AI  FF ILLSATVAY VSSD +    S++ED+            P    ++FQ+ 
Sbjct: 2   ERFNAIPLFFAILLSATVAYGVSSDQINSAVSDEEDILIRQVVSGADDRPLTAEQHFQDF 61

Query: 76  ELKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIR 135
           +LKFGK Y  DEEHDYRFR    A  R  +R                      +R   + 
Sbjct: 62  KLKFGKTYTTDEEHDYRFRV-FKANLRKAKRHQKLDPDAVHGVTRFSDLTESEFRENFVG 121

Query: 136 SHRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFR 195
            +R+ +P    ++   P+    S+               GS  S W  S +  L    F 
Sbjct: 122 LNRLRLPADAHQAPILPTDNLASDFDWRDQGAVTPVKDQGSCGSCWSFSAVGALEGANFL 181

Query: 196 PAGQRA----------------------------------------AGGLERQEDYPSTG 239
             G+                                          AGGLER+EDYP TG
Sbjct: 182 STGKLISLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYIVKAGGLEREEDYPYTG 241

[Close]

BLAST of Cp4.1LG11g02250 vs. NCBI nr
Match: XP_022951324.1 (cysteine protease RD19A-like [Cucurbita moschata] >XP_022951325.1 cysteine protease RD19A-like [Cucurbita moschata])

HSP 1 Score: 127 bits (318), Expect = 1.41e-30
Identity = 118/372 (31.72%), Postives = 150/372 (40.32%), Query Frame = 0


Query: 16  ERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL-------SSPYRLA---KNFQNS 75
           ERL  IA  F ILLSATVAY  SSD + G  +ED+D+        +  RL    ++FQN 
Sbjct: 2   ERLTVIALSFAILLSATVAYGESSDQING--AEDDDILIRQVVSGADDRLLTAEQHFQNF 61

Query: 76  ELKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIR 135
           +LKFGK YA DEEHDYRFR    A  R  +R                      +R   + 
Sbjct: 62  KLKFGKTYATDEEHDYRFRI-FKANLRKAKRHQKLDPGAVHGVTQFSDLTEDEFRKNFVG 121

Query: 136 SHRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFR 195
            +R+ +P   +++   P+    S+               GS  S W  S +  L    F 
Sbjct: 122 VNRLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFL 181

Query: 196 PAGQRA----------------------------------------AGGLERQEDYPSTG 240
             GQ                                          AGGLER+EDYP TG
Sbjct: 182 STGQLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMNSAFEYILKAGGLEREEDYPYTG 241

[Close]

BLAST of Cp4.1LG11g02250 vs. NCBI nr
Match: XP_023002391.1 (cysteine protease RD19A-like [Cucurbita maxima])

HSP 1 Score: 127 bits (318), Expect = 1.41e-30
Identity = 119/372 (31.99%), Postives = 149/372 (40.05%), Query Frame = 0


Query: 16  ERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL-------SSPYRLA---KNFQNS 75
           ERL  IA  F ILLSATVAY VSSD + G   ED+DL           RL    ++FQN 
Sbjct: 2   ERLTVIALSFAILLSATVAYGVSSDQING--VEDDDLLIRQVVSGDDDRLLTAEQHFQNF 61

Query: 76  ELKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIR 135
           +LKFGK YA DEEHDYRFR    A  R  +R                      +R   + 
Sbjct: 62  KLKFGKTYATDEEHDYRFRI-FKANLRKAKRHQKLDPGAIHGVTQFSDLTEDEFRKNFVG 121

Query: 136 SHRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFR 195
            +R+ +P   +++   P+    S+               GS  S W  S +  L    F 
Sbjct: 122 VNRLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFL 181

Query: 196 PAGQRA----------------------------------------AGGLERQEDYPSTG 240
             G+                                          AGGLER+EDYP TG
Sbjct: 182 STGKLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTG 241

[Close]

BLAST of Cp4.1LG11g02250 vs. NCBI nr
Match: KAA0065192.1 (cysteine proteinase RD19a-like [Cucumis melo var. makuwa])

HSP 1 Score: 125 bits (315), Expect = 3.84e-30
Identity = 119/369 (32.25%), Postives = 148/369 (40.11%), Query Frame = 0


Query: 17  RLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL-------SSPYRLA---KNFQNSE 76
           R  AIA FF ILLSATVAY V SD + G  S+ EDL        +  RL    ++FQN +
Sbjct: 3   RFNAIALFFAILLSATVAYGVFSDQINGDVSDGEDLLIRQVVSGADDRLLTAEQHFQNFK 62

Query: 77  LKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIRS 136
           LKFGK YA  EEHDYRFR    A  R  +R                      +R   +  
Sbjct: 63  LKFGKTYATAEEHDYRFRV-FKANLRKAKRHQKLDPDAVHGVTRFSDLTESEFRENFVGL 122

Query: 137 HRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFRP 196
           +R+ +P    ++   P+    S+               GS  S W  S +  L    F  
Sbjct: 123 NRLRLPADAHQAPILPTDNLASDFDWRDKGAVTPVKDQGSCGSCWSFSAVGALEGANFLS 182

Query: 197 AGQRA------------------------------------AGGLERQEDYPSTGRYRGP 240
            G+                                      AGGLER+EDYP TG  RG 
Sbjct: 183 TGKLISLSEQQLVDCDHEVPGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTGTDRGS 242

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy TrEMBL
Match: A0A0A0LPD9 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G363570 PE=3 SV=1)

HSP 1 Score: 129 bits (323), Expect = 1.33e-31
Identity = 115/371 (31.00%), Postives = 145/371 (39.08%), Query Frame = 0


Query: 16  ERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL----------SSPYRLAKNFQNS 75
           ER  AI  FF ILLSATVAY VSSD +    S++ED+            P    ++FQ+ 
Sbjct: 2   ERFNAIPLFFAILLSATVAYGVSSDQINSAVSDEEDILIRQVVSGADDRPLTAEQHFQDF 61

Query: 76  ELKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIR 135
           +LKFGK Y  DEEHDYRFR    A  R  +R                      +R   + 
Sbjct: 62  KLKFGKTYTTDEEHDYRFRV-FKANLRKAKRHQKLDPDAVHGVTRFSDLTESEFRENFVG 121

Query: 136 SHRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFR 195
            +R+ +P    ++   P+    S+               GS  S W  S +  L    F 
Sbjct: 122 LNRLRLPADAHQAPILPTDNLASDFDWRDQGAVTPVKDQGSCGSCWSFSAVGALEGANFL 181

Query: 196 PAGQRA----------------------------------------AGGLERQEDYPSTG 239
             G+                                          AGGLER+EDYP TG
Sbjct: 182 STGKLISLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYIVKAGGLEREEDYPYTG 241

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy TrEMBL
Match: A0A6J1GIG1 (cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111454190 PE=3 SV=1)

HSP 1 Score: 127 bits (318), Expect = 6.83e-31
Identity = 118/372 (31.72%), Postives = 150/372 (40.32%), Query Frame = 0


Query: 16  ERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL-------SSPYRLA---KNFQNS 75
           ERL  IA  F ILLSATVAY  SSD + G  +ED+D+        +  RL    ++FQN 
Sbjct: 2   ERLTVIALSFAILLSATVAYGESSDQING--AEDDDILIRQVVSGADDRLLTAEQHFQNF 61

Query: 76  ELKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIR 135
           +LKFGK YA DEEHDYRFR    A  R  +R                      +R   + 
Sbjct: 62  KLKFGKTYATDEEHDYRFRI-FKANLRKAKRHQKLDPGAVHGVTQFSDLTEDEFRKNFVG 121

Query: 136 SHRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFR 195
            +R+ +P   +++   P+    S+               GS  S W  S +  L    F 
Sbjct: 122 VNRLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFL 181

Query: 196 PAGQRA----------------------------------------AGGLERQEDYPSTG 240
             GQ                                          AGGLER+EDYP TG
Sbjct: 182 STGQLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMNSAFEYILKAGGLEREEDYPYTG 241

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy TrEMBL
Match: A0A6J1KL64 (cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111496246 PE=3 SV=1)

HSP 1 Score: 127 bits (318), Expect = 6.83e-31
Identity = 119/372 (31.99%), Postives = 149/372 (40.05%), Query Frame = 0


Query: 16  ERLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL-------SSPYRLA---KNFQNS 75
           ERL  IA  F ILLSATVAY VSSD + G   ED+DL           RL    ++FQN 
Sbjct: 2   ERLTVIALSFAILLSATVAYGVSSDQING--VEDDDLLIRQVVSGDDDRLLTAEQHFQNF 61

Query: 76  ELKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIR 135
           +LKFGK YA DEEHDYRFR    A  R  +R                      +R   + 
Sbjct: 62  KLKFGKTYATDEEHDYRFRI-FKANLRKAKRHQKLDPGAIHGVTQFSDLTEDEFRKNFVG 121

Query: 136 SHRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFR 195
            +R+ +P   +++   P+    S+               GS  S W  S +  L    F 
Sbjct: 122 VNRLRLPADAQKAPILPTDNLASDFDWRDHGAVTPVKNQGSCGSCWSFSAVGALEGANFL 181

Query: 196 PAGQRA----------------------------------------AGGLERQEDYPSTG 240
             G+                                          AGGLER+EDYP TG
Sbjct: 182 STGKLVSLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTG 241

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy TrEMBL
Match: A0A5A7VA47 (Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005290 PE=3 SV=1)

HSP 1 Score: 125 bits (315), Expect = 1.86e-30
Identity = 119/369 (32.25%), Postives = 148/369 (40.11%), Query Frame = 0


Query: 17  RLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL-------SSPYRLA---KNFQNSE 76
           R  AIA FF ILLSATVAY V SD + G  S+ EDL        +  RL    ++FQN +
Sbjct: 3   RFNAIALFFAILLSATVAYGVFSDQINGDVSDGEDLLIRQVVSGADDRLLTAEQHFQNFK 62

Query: 77  LKFGKAYAIDEEHDYRFRSQAPAGARSWRR---------------------PWRHVVIRS 136
           LKFGK YA  EEHDYRFR    A  R  +R                      +R   +  
Sbjct: 63  LKFGKTYATAEEHDYRFRV-FKANLRKAKRHQKLDPDAVHGVTRFSDLTESEFRENFVGL 122

Query: 137 HRVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFRP 196
           +R+ +P    ++   P+    S+               GS  S W  S +  L    F  
Sbjct: 123 NRLRLPADAHQAPILPTDNLASDFDWRDKGAVTPVKDQGSCGSCWSFSAVGALEGANFLS 182

Query: 197 AGQRA------------------------------------AGGLERQEDYPSTGRYRGP 240
            G+                                      AGGLER+EDYP TG  RG 
Sbjct: 183 TGKLISLSEQQLVDCDHEVPGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTGTDRGS 242

[Close]

BLAST of Cp4.1LG11g02250 vs. ExPASy TrEMBL
Match: A0A1S3BB40 (cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103488015 PE=3 SV=1)

HSP 1 Score: 122 bits (307), Expect = 2.86e-29
Identity = 118/372 (31.72%), Postives = 146/372 (39.25%), Query Frame = 0


Query: 17  RLAAIAFFFTILLSATVAYDVSSDLMYGGDSEDEDL-------SSPYRLA---KNFQNSE 76
           R  AIA FF ILLSATVAY V SD + G  S+ EDL        +  RL    + FQN +
Sbjct: 3   RFNAIALFFAILLSATVAYGVFSDQIDGDVSDGEDLLIRQVVSGADDRLLTAEQRFQNFK 62

Query: 77  LKFGKAYAIDEEHDYRFR----------------SQAPAGARSW----RRPWRHVVIRSH 136
           LKFGK YA  EEHDYRFR                  A  G   +       +R   +  +
Sbjct: 63  LKFGKTYATAEEHDYRFRVFKDNLRKAKRHQKLDPDAVHGVTRFSDLTESEFRENFVGLN 122

Query: 137 RVLVPYTFRRSESAPSFCRFSE---------------GSDPSYWRPSLLLRLTLPRFRPA 196
           R+ +P    ++   P+    S+               GS  S W  S +  L    F   
Sbjct: 123 RLRLPADAHQAPILPTDNLASDFDWRDKGAVTPVKDQGSCGSCWSFSAVGALEGANFLST 182

Query: 197 GQRA----------------------------------------AGGLERQEDYPSTGRY 240
           G+                                          AGGLER+EDYP TG  
Sbjct: 183 GKLISLSEQQLVDCDHECDPEEAGACDAGCNGGLMTSAFEYILKAGGLEREEDYPYTGTD 242

[Close]

BLAST of Cp4.1LG11g02250 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 63.5 bits (153), Expect = 2.6e-10
Identity = 42/141 (29.79%), Postives = 57/141 (40.43%), Query Frame = 0


Query: 157 GGLERQEDYPSTGRYRGPCKFQ---------------------SGIKMPTKTAAVGVDAI 216
           GGL R++DYP TG   G CK                       +   +     AV ++A 
Sbjct: 219 GGLMREKDYPYTGTDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAA 278

Query: 217 FLQTNVRGISCV--------------------------------------GENWGENGYY 239
           ++QT + G+SC                                       GE+WGENG+Y
Sbjct: 279 YMQTYIGGVSCPYICSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFY 338

[Close]

BLAST of Cp4.1LG11g02250 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 63.2 bits (152), Expect = 3.4e-10
Identity = 45/146 (30.82%), Postives = 59/146 (40.41%), Query Frame = 0


Query: 156 AGGLERQEDYPSTGRYRGPCKFQSGIKMPTKTA--------------------------A 215
           AGGLE +  YP TG+ RG CKF      P K A                          A
Sbjct: 223 AGGLEEERSYPYTGK-RGHCKFD-----PEKVAVRVLNFTTIPLDENQIAANLVRHGPLA 282

Query: 216 VGVDAIFLQTNVRGISCV---------------------------------------GEN 237
           VG++A+F+QT + G+SC                                        G+ 
Sbjct: 283 VGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKK 342

[Close]

BLAST of Cp4.1LG11g02250 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 62.0 bits (149), Expect = 7.5e-10
Identity = 42/141 (29.79%), Postives = 57/141 (40.43%), Query Frame = 0


Query: 157 GGLERQEDYPSTGRYRGPCKFQSGIKMPTKT---------------------AAVGVDAI 216
           GGL ++EDYP TG+    CK      + + +                      AV ++A 
Sbjct: 222 GGLMKEEDYPYTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAG 281

Query: 217 FLQTNVRGISCV--------------------------------------GENWGENGYY 239
           ++QT + G+SC                                       GE WGENG+Y
Sbjct: 282 YMQTYIGGVSCPYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFY 341

[Close]

BLAST of Cp4.1LG11g02250 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 57.4 bits (137), Expect = 1.9e-08
Identity = 40/142 (28.17%), Postives = 59/142 (41.55%), Query Frame = 0


Query: 156 AGGLERQEDYPSTGRYRGPCKFQSGIKMPTKT---------------------AAVGVDA 215
           AGGL ++EDYP TGR    CKF     + + +                      A+ ++A
Sbjct: 226 AGGLMKEEDYPYTGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINA 285

Query: 216 IFLQTNVRGISCV--------------------------------------GENWGENGY 238
           +++QT + G+SC                                       G  WGE+GY
Sbjct: 286 MWMQTYIGGVSCPYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGY 345

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g02250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43295	3.6e-09	29.79	Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S...	[more]
Q8VYS0	4.8e-09	30.82	Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 S...	[more]
Q10716	8.1e-09	31.25	Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1	[more]
P43296	1.1e-08	29.79	Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1	[more]
Q9SUL1	2.6e-07	28.17	Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S...	[more]

BLAST of Cp4.1LG11g02250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884968.1	5.87e-36	33.06	cysteine protease RD19A-like [Benincasa hispida]	[more]
XP_004152668.1	2.75e-31	31.00	cysteine protease RD19A [Cucumis sativus] >KGN62647.1 hypothetical protein Csa_0...	[more]
XP_022951324.1	1.41e-30	31.72	cysteine protease RD19A-like [Cucurbita moschata] >XP_022951325.1 cysteine prote...	[more]
XP_023002391.1	1.41e-30	31.99	cysteine protease RD19A-like [Cucurbita maxima]	[more]
KAA0065192.1	3.84e-30	32.25	cysteine proteinase RD19a-like [Cucumis melo var. makuwa]	[more]

BLAST of Cp4.1LG11g02250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPD9	1.33e-31	31.00	Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G363570 PE=3 SV=1	[more]
A0A6J1GIG1	6.83e-31	31.72	cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111454190 PE=3 ...	[more]
A0A6J1KL64	6.83e-31	31.99	cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111496246 PE=3 SV...	[more]
A0A5A7VA47	1.86e-30	32.25	Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A1S3BB40	2.86e-29	31.72	cysteine proteinase RD19a-like OS=Cucumis melo OX=3656 GN=LOC103488015 PE=3 SV=1	[more]

BLAST of Cp4.1LG11g02250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21430.1	2.6e-10	29.79	Papain family cysteine protease	[more]
AT3G54940.2	3.4e-10	30.82	Papain family cysteine protease	[more]
AT4G39090.1	7.5e-10	29.79	Papain family cysteine protease	[more]
AT4G16190.1	1.9e-08	28.17	Papain family cysteine protease	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.40.50.170	Cysteine proteinases. Chain C	coord: 193..239 e-value: 4.2E-5 score: 25.4
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 208..236

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g02250.1	Cp4.1LG11g02250.1	mRNA