Cp4.1LG11g01670 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g01670
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	DPH4 homolog
Location	Cp4.1LG11: 901413 .. 901952 (+)
RNA-Seq Expression	Cp4.1LG11g01670
Synteny	Cp4.1LG11g01670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCAAGCAGTGGCTCCATTGATGAAACTTATTATGATGTACTGTCTTTGAGGGAAGATGCTAGCTACGATGAAATAAGAGCCAGCTATCGTTCTTCTCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATTTAGACGACATTGCGGGAGAAAGATACTTGAAGGTGCAGAAGGCTTGGGAAGTCCTCAGCAGCTCAATGTCCCGTGCAGCTTATGACAGAGAGCTCCAAGTTGCCAAAGGCGATGCAATTGGTGCAGAGAGCATAAGGTTGGAGGATATGGGTGTGGAAGATAAAGGTGAAGTTGTAGAACTCCTTTATCAGTGCCGTTGTGGAGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATGTCCATTGTTGAGGAATGGGAGAAAGGTTTCTTTAAGGACTCTGGATGTGTTGCCTGCTTCCGTTGTTTTTCCTTGTGGTTCTTGCTCTTTGAAAGTTCGTTTACTTATTGATTCGAACTCAAGTGTTTTGAATGATTGA

mRNA sequence

ATGAGTTCAAGCAGTGGCTCCATTGATGAAACTTATTATGATGTACTGTCTTTGAGGGAAGATGCTAGCTACGATGAAATAAGAGCCAGCTATCGTTCTTCTCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATTTAGACGACATTGCGGGAGAAAGATACTTGAAGGTGCAGAAGGCTTGGGAAGTCCTCAGCAGCTCAATGTCCCGTGCAGCTTATGACAGAGAGCTCCAAGTTGCCAAAGGCGATGCAATTGGTGCAGAGAGCATAAGGTTGGAGGATATGGGTGTGGAAGATAAAGGTGAAGTTGTAGAACTCCTTTATCAGTGCCGTTGTGGAGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATGTCCATTGTTGAGGAATGGGAGAAAGGTTTCTTTAAGGACTCTGGATGTGTTGCCTGCTTCCGTTGTTTTTCCTTGTGGTTCTTGCTCTTTGAAAGTTCGTTTACTTATTGATTCGAACTCAAGTGTTTTGAATGATTGA

Coding sequence (CDS)

ATGAGTTCAAGCAGTGGCTCCATTGATGAAACTTATTATGATGTACTGTCTTTGAGGGAAGATGCTAGCTACGATGAAATAAGAGCCAGCTATCGTTCTTCTCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATTTAGACGACATTGCGGGAGAAAGATACTTGAAGGTGCAGAAGGCTTGGGAAGTCCTCAGCAGCTCAATGTCCCGTGCAGCTTATGACAGAGAGCTCCAAGTTGCCAAAGGCGATGCAATTGGTGCAGAGAGCATAAGGTTGGAGGATATGGGTGTGGAAGATAAAGGTGAAGTTGTAGAACTCCTTTATCAGTGCCGTTGTGGAGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATGTCCATTGTTGAGGAATGGGAGAAAGGTTTCTTTAAGGACTCTGGATGTGTTGCCTGCTTCCGTTGTTTTTCCTTGTGGTTCTTGCTCTTTGAAAGTTCGTTTACTTATTGATTCGAACTCAAGTGTTTTGAATGATTGA

Protein sequence

MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGDYFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND

Homology

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BLAST of Cp4.1LG11g01670 vs. ExPASy Swiss-Prot
Match: Q54CI5 (DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.3e-10
Identity = 45/164 (27.44%), Postives = 80/164 (48.78%), Query Frame = 0


Query: 2   SSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQ---------------AM 61
           ++S+   ++ YY++L +  DA  +EI+ SYR   L +HPDKL                 +
Sbjct: 6   NNSNKCENKNYYEILKVSIDADIEEIKKSYRKLALLYHPDKLNKEENIEENINNFSNCLV 65

Query: 62  CHKSHLDDIAGERYLKVQKAWEVLSSSMSRAAYDRELQVAKGDAIG-AESIRLEDMG-VE 121
            + ++ ++   + +  +Q AWE L   + R  YD  L   K      ++ I L+DM  +E
Sbjct: 66  NNNNNNNNSNTKDFNDIQIAWETLKDDLLRKQYDSLLLEQKRQKYSVSDEIDLDDMEFIE 125

Query: 122 DKGEVVELLYQCRCGDYFFIDSGELDEMGCPLLRNGRKVSLRTL 149
           +  E V   Y CRCGD++ I   +L E    +  +G  +S++ +
Sbjct: 126 ENSEYV---YPCRCGDHYIITEDQLSEGSDVVCCSGCSLSIKVI 166

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy Swiss-Prot
Match: P47138 (Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=JJJ3 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.5e-09
Identity = 49/173 (28.32%), Postives = 80/173 (46.24%), Query Frame = 0


Query: 11  TYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVL 70
           T+Y++L +  DA+ DEI+ +YR+ LLN HPDKL    H    D ++     K+Q A+++L
Sbjct: 8   THYEILRIPSDATQDEIKKAYRNRLLNTHPDKLSKSIH----DTVSNVTINKIQDAYKIL 67

Query: 71  SSSMSRAAYDR------ELQVAKGDAIGAESIRLEDMGV-EDKGEVVELLYQCRCGDYFF 130
           S+  +R  YDR      + Q       G +   L+D    EDK E +    +C+    F 
Sbjct: 68  SNIKTRREYDRLILENYKRQGFHNCGDGLDEFSLDDFSFDEDKLEFMMNCPRCQFVGGFH 127

Query: 131 IDSGELDEMGCPLLRNGRKVSLRTLDVLPAS-----VVFPCGSCSLKVRLLID 172
                LDE             +  +D +  S     ++  C +CSL +++  D
Sbjct: 128 FSESLLDE------------CIDNVDAMERSHSGYQLLTQCSACSLWLKVNFD 164

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy Swiss-Prot
Match: Q6FWM1 (Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=DPH4 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.0e-09
Identity = 43/130 (33.08%), Postives = 68/130 (52.31%), Query Frame = 0


Query: 11  TYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVL 70
           ++Y+VL +  DAS DEI+ +YR  LL  HPDK + +   S +   +G    ++Q+A+ VL
Sbjct: 10  SHYEVLGVPVDASTDEIKLTYRKMLLTLHPDKSKTVPIDSTIKIRSGVSINQIQEAYRVL 69

Query: 71  SSSMSRAAYDRELQVAK--------GDAIGAESIRLEDMGV-EDKGEVVELLYQCRCGDY 130
           S+   RAAY+R L  +         GD  G +   L++    E+K E V    +C+ G  
Sbjct: 70  SNDQLRAAYNRALNDSNKLAGFNNFGD--GLDEFNLDEFEFNEEKLEYVMTCPRCQSGGG 129

Query: 131 FFIDSGELDE 132
           F +    L+E
Sbjct: 130 FVLSEDMLEE 137

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy Swiss-Prot
Match: B9KH92 (Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 29/70 (41.43%), Postives = 43/70 (61.43%), Query Frame = 0


Query: 12 YYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVLS 71
          YY++L +  +AS +EI+ SYR  +  +HPDK          D  A E++ K+ +A+EVLS
Sbjct: 6  YYEILEVSRNASAEEIKKSYRKMVFKYHPDKNPG-------DKKAEEKFKKISEAYEVLS 65

Query: 72 SSMSRAAYDR 82
          +   RAAYDR
Sbjct: 66 NPEKRAAYDR 68

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy Swiss-Prot
Match: Q5P9E0 (Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 29/70 (41.43%), Postives = 43/70 (61.43%), Query Frame = 0


Query: 12 YYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVLS 71
          YY++L +  +AS +EI+ SYR  +  +HPDK          D  A E++ K+ +A+EVLS
Sbjct: 6  YYEILEVSRNASAEEIKKSYRKMVFKYHPDKNPG-------DKKAEEKFKKISEAYEVLS 65

Query: 72 SSMSRAAYDR 82
          +   RAAYDR
Sbjct: 66 NPEKRAAYDR 68

[Close]

BLAST of Cp4.1LG11g01670 vs. NCBI nr
Match: XP_023546755.1 (DPH4 homolog [Cucurbita pepo subsp. pepo] >XP_023546756.1 DPH4 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357 bits (915), Expect = 4.43e-124
Identity = 179/179 (100.00%), Postives = 179/179 (100.00%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY
Sbjct: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
           LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND
Sbjct: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179

[Close]

BLAST of Cp4.1LG11g01670 vs. NCBI nr
Match: XP_022996470.1 (DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima])

HSP 1 Score: 341 bits (874), Expect = 8.50e-118
Identity = 172/179 (96.09%), Postives = 173/179 (96.65%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSI ETYYDVLSLREDASYDEIRASYRS+LLNFHPDKLQAMCHKSHLDDIAGERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
           LKVQKAWEVLSSSMSRAAYDRELQ AKGDAIGAESIRLEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGELDEMG PLLRNGRKVSLRTLDVLPASVV PCGSCSLKVRLLIDSNSSVLND
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSNSSVLND 179

[Close]

BLAST of Cp4.1LG11g01670 vs. NCBI nr
Match: XP_022962179.1 (DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moschata] >KAG7029347.1 JJJ3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 339 bits (870), Expect = 3.23e-117
Identity = 171/179 (95.53%), Postives = 173/179 (96.65%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSI ETYYDVLSLREDASYDEIRASYRS+LLNFHPDKLQAMCHKSHLDDIAGERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
           LKVQKAWEVLSSSMSRAAYDRELQ AKGDAIGAESIRLEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGELDEMG PLLRNGRKVSLRTLDVLPASVV PCGSCSL+VRLLIDSNSSVLND
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSNSSVLND 179

[Close]

BLAST of Cp4.1LG11g01670 vs. NCBI nr
Match: XP_038884890.1 (DPH4 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 316 bits (809), Expect = 6.43e-108
Identity = 161/179 (89.94%), Postives = 164/179 (91.62%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSI ETYYDVLSL+EDASYDEIRASYRS+LLNFHPDKLQAMCHKSH DDIA ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SSMSRA+YDRELQ AKGDAIGAESI LEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGELDEMG PLLRNG KVSLRTLD LPASVV PCGSCSLKVRLLIDSNSSV  D
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 179

[Close]

BLAST of Cp4.1LG11g01670 vs. NCBI nr
Match: KAG6598383.1 (hypothetical protein SDJN03_08161, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 310 bits (794), Expect = 2.86e-105
Identity = 154/162 (95.06%), Postives = 155/162 (95.68%), Query Frame = 0


Query: 18  LREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVLSSSMSRA 77
           L  DASYDEIRASYRS+LLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVLSSSMSRA
Sbjct: 42  LLSDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEVLSSSMSRA 101

Query: 78  AYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGDYFFIDSGELDEMGCPLL 137
           AYDRELQ AKGDAIGAESIRLEDM VEDKGEVVEL YQCRCGDYFFIDSGELDEMG PLL
Sbjct: 102 AYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGDYFFIDSGELDEMGYPLL 161

Query: 138 RNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           RNGRKVSLRTLDVLPASVV PCGSCSLKVRLLIDSNSSVLND
Sbjct: 162 RNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSNSSVLND 203

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy TrEMBL
Match: A0A6J1K4U3 (DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1)

HSP 1 Score: 341 bits (874), Expect = 4.11e-118
Identity = 172/179 (96.09%), Postives = 173/179 (96.65%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSI ETYYDVLSLREDASYDEIRASYRS+LLNFHPDKLQAMCHKSHLDDIAGERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
           LKVQKAWEVLSSSMSRAAYDRELQ AKGDAIGAESIRLEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGELDEMG PLLRNGRKVSLRTLDVLPASVV PCGSCSLKVRLLIDSNSSVLND
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSNSSVLND 179

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy TrEMBL
Match: A0A6J1HGB5 (DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1)

HSP 1 Score: 339 bits (870), Expect = 1.56e-117
Identity = 171/179 (95.53%), Postives = 173/179 (96.65%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSI ETYYDVLSLREDASYDEIRASYRS+LLNFHPDKLQAMCHKSHLDDIAGERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
           LKVQKAWEVLSSSMSRAAYDRELQ AKGDAIGAESIRLEDM VEDKGEVVEL YQCRCGD
Sbjct: 61  LKVQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGELDEMG PLLRNGRKVSLRTLDVLPASVV PCGSCSL+VRLLIDSNSSVLND
Sbjct: 121 YFFIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSNSSVLND 179

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy TrEMBL
Match: A0A0A0LKX8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1)

HSP 1 Score: 306 bits (784), Expect = 1.64e-104
Identity = 154/173 (89.02%), Postives = 159/173 (91.91%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           M+S SGSI ETYYDVLSLREDAS+DEIRASYRS+LLNFHPDKLQAMCHKSH DDI GERY
Sbjct: 1   MNSISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SS SRA+YDRELQ AKGDAIGAESI LEDM VEDKGEVVELLYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSN 173
           YFFIDSGELDEMG PLLRNG KVSLRTL+ LPASVV PCGSCSLKVRLLIDSN
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 173

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy TrEMBL
Match: A0A6J1KK19 (DPH4 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1)

HSP 1 Score: 303 bits (775), Expect = 4.91e-103
Identity = 152/179 (84.92%), Postives = 162/179 (90.50%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSI ETYYDVLSLREDAS+DEIRASYRS+LLNFHPDKLQAMC KSH +D+ GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 60

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SSMSRAAYDRELQ AKG+A+GAESI LEDM VEDKGEVVELLYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 120

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGEL+EMG P+LR G KVSLRTLD LPAS+V PCGSCSLKVRLLIDS+S V  D
Sbjct: 121 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSID 179

[Close]

BLAST of Cp4.1LG11g01670 vs. ExPASy TrEMBL
Match: A0A6J1KMK1 (DPH4 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1)

HSP 1 Score: 303 bits (775), Expect = 1.89e-102
Identity = 152/179 (84.92%), Postives = 162/179 (90.50%), Query Frame = 0


Query: 1   MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
           MSSSSGSI ETYYDVLSLREDAS+DEIRASYRS+LLNFHPDKLQAMC KSH +D+ GERY
Sbjct: 40  MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 99

Query: 61  LKVQKAWEVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGD 120
            KVQKAWEVL SSMSRAAYDRELQ AKG+A+GAESI LEDM VEDKGEVVELLYQCRCGD
Sbjct: 100 FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 159

Query: 121 YFFIDSGELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSVLND 179
           YFFIDSGEL+EMG P+LR G KVSLRTLD LPAS+V PCGSCSLKVRLLIDS+S V  D
Sbjct: 160 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSID 218

[Close]

BLAST of Cp4.1LG11g01670 vs. TAIR 10
Match: AT4G10130.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 181.8 bits (460), Expect = 4.9e-46
Identity = 89/169 (52.66%), Postives = 119/169 (70.41%), Query Frame = 0


Query: 8   IDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAW 67
           + ETYY++LS++EDASY+EIR SYRS++L+ HPDKL      S  D    E++LK+QKAW
Sbjct: 8   VHETYYEILSVKEDASYEEIRNSYRSAILHSHPDKLNNTSRSSSDD----EKFLKIQKAW 67

Query: 68  EVLSSSMSRAAYDRELQVAKGDAIGAESIRLEDMGVEDKGEVVELLYQCRCGDYFFIDSG 127
           EVLS +  R  YD +L+ ++ D I A+ I +EDM VE  G+V++L YQCRCGDYF +DS 
Sbjct: 68  EVLSDAELRVVYDNDLRSSRHDGITADEISIEDMSVEITGDVIDLFYQCRCGDYFCVDSS 127

Query: 128 ELDEMGCPLLRNGRKVSLRTLDVLPASVVFPCGSCSLKVRLLIDSNSSV 177
           EL  MG  LLR+G  V ++ L    ASVV PCGSCSLK R+ +DS+  +
Sbjct: 128 ELGTMGFALLRDGDFVYVKRLGAFVASVVLPCGSCSLKTRVWVDSDMKI 172

[Close]

BLAST of Cp4.1LG11g01670 vs. TAIR 10
Match: AT1G74250.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.4e-07
Identity = 33/80 (41.25%), Postives = 47/80 (58.75%), Query Frame = 0


Query: 1  MSSSSGSIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERY 60
          M+SSS S    +Y+VL + +++S DEIR+SYR   L  HPDKL      S  +  A  ++
Sbjct: 1  MASSSRSEKRCHYEVLGISKESSPDEIRSSYRRLALQRHPDKLMKAAGLSEAE--ATAQF 60

Query: 61 LKVQKAWEVLSSSMSRAAYD 81
           ++  A+EVLS    RA YD
Sbjct: 61 QELVHAYEVLSDPKERAWYD 78

[Close]

BLAST of Cp4.1LG11g01670 vs. TAIR 10
Match: AT1G77020.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 50.1 bits (118), Expect = 2.2e-06
Identity = 28/73 (38.36%), Postives = 40/73 (54.79%), Query Frame = 0


Query: 9  DETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWE 68
          +  YYDVL +   AS +EIR +Y       HPDK Q        D +A E++  + +A++
Sbjct: 4  ETVYYDVLGVTPSASEEEIRKAYYIKARQVHPDKNQG-------DPLAAEKFQVLGEAYQ 63

Query: 69 VLSSSMSRAAYDR 82
          VLS  + R AYDR
Sbjct: 64 VLSDPVHREAYDR 69

[Close]

BLAST of Cp4.1LG11g01670 vs. TAIR 10
Match: AT1G56300.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.8e-06
Identity = 25/77 (32.47%), Postives = 45/77 (58.44%), Query Frame = 0


Query: 7  SIDETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGE---RYLKV 66
          ++  +YY +L +R+DAS  +IR +YR   + +HPD+       +    +AGE   R+ ++
Sbjct: 9  NVRSSYYTILGIRKDASVSDIRTAYRKLAMKWHPDRY------ARNPGVAGEAKRRFQQI 68

Query: 67 QKAWEVLSSSMSRAAYD 81
          Q+A+ VL+    R+ YD
Sbjct: 69 QEAYSVLNDENKRSMYD 79

[Close]

BLAST of Cp4.1LG11g01670 vs. TAIR 10
Match: AT4G37480.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.8e-06
Identity = 28/74 (37.84%), Postives = 42/74 (56.76%), Query Frame = 0


Query: 10  ETYYDVLSLREDASYDEIRASYRSSLLNFHPDKLQAMCHKSHLDDIAGERYLKVQKAWEV 69
           E  YD+L++ E +S  EI+AS+R      HPD ++     S  D     R++++  A+E+
Sbjct: 55  ENAYDILNVSETSSIAEIKASFRRLAKETHPDLIE-----SKKDPSNSRRFVQILAAYEI 114

Query: 70  LSSSMSRAAYDREL 84
           LS S  RA YDR L
Sbjct: 115 LSDSEKRAHYDRYL 123

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g01670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54CI5	9.3e-10	27.44	DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1	[more]
P47138	3.5e-09	28.32	Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
Q6FWM1	6.0e-09	33.08	Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 1...	[more]
B9KH92	2.5e-07	41.43	Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ...	[more]
Q5P9E0	2.5e-07	41.43	Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=d...	[more]

BLAST of Cp4.1LG11g01670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023546755.1	4.43e-124	100.00	DPH4 homolog [Cucurbita pepo subsp. pepo] >XP_023546756.1 DPH4 homolog [Cucurbit...	[more]
XP_022996470.1	8.50e-118	96.09	DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima]	[more]
XP_022962179.1	3.23e-117	95.53	DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moscha...	[more]
XP_038884890.1	6.43e-108	89.94	DPH4 homolog isoform X2 [Benincasa hispida]	[more]
KAG6598383.1	2.86e-105	95.06	hypothetical protein SDJN03_08161, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cp4.1LG11g01670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K4U3	4.11e-118	96.09	DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1	[more]
A0A6J1HGB5	1.56e-117	95.53	DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1	[more]
A0A0A0LKX8	1.64e-104	89.02	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1	[more]
A0A6J1KK19	4.91e-103	84.92	DPH4 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1	[more]
A0A6J1KMK1	1.89e-102	84.92	DPH4 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1	[more]

BLAST of Cp4.1LG11g01670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10130.1	4.9e-46	52.66	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G74250.1	3.4e-07	41.25	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G77020.1	2.2e-06	38.36	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G56300.1	3.8e-06	32.47	Chaperone DnaJ-domain superfamily protein	[more]
AT4G37480.1	3.8e-06	37.84	Chaperone DnaJ-domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 55..75 score: 29.33 coord: 13..31 score: 38.0 coord: 31..46 score: 34.55
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 10..75 e-value: 1.5E-9 score: 47.7
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 11..80 e-value: 9.8E-15 score: 54.4
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 11..83 score: 15.123656
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 11..71 e-value: 6.30443E-13 score: 58.7109
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 5..86 e-value: 1.0E-17 score: 66.1
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 10..83
IPR036671	DPH-type metal-binding domain superfamily	GENE3D	3.10.660.10	DPH Zinc finger	coord: 87..179 e-value: 2.7E-15 score: 57.6
IPR036671	DPH-type metal-binding domain superfamily	SUPERFAMILY	144217	CSL zinc finger	coord: 92..171
IPR007872	DPH-type metal-binding domain	PFAM	PF05207	zf-CSL	coord: 95..169 e-value: 4.2E-12 score: 45.6
IPR007872	DPH-type metal-binding domain	PROSITE	PS51074	DPH_MB	coord: 92..171 score: 14.298623
None	No IPR available	PANTHER	PTHR21454:SF31	DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4	coord: 1..172
IPR044248	Diphthamide biosynthesis protein 3/4-like	PANTHER	PTHR21454	DPH3 HOMOLOG-RELATED	coord: 1..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g01670.1	Cp4.1LG11g01670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding