Cp4.1LG10g12300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG10g12300 vs. ExPASy Swiss-Prot
Match: P0DH90 (Protein FRIGIDA OS=Arabidopsis thaliana OX=3702 GN=FRI PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.1e-76
Identity = 172/457 (37.64%), Postives = 264/457 (57.77%), Query Frame = 0

Query: 42  LGSESEPQETQFPFLNFSYIDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLIS 101
           +G   +PQ     FL    IDEL + S A+  F+ +  +LQ H+ SI NAID++      
Sbjct: 47  IGQSKQPQ-----FL--KSIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSK------ 106

Query: 102 SSNRLLDNHRQLLSVGDGKIALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSD 161
                L+++  +L+  +     P                      +L  PRNN+   ++ 
Sbjct: 107 -----LESNGVVLAARNNNFHQP----------------------MLSPPRNNVSVETTV 166

Query: 162 FGKNDGKEAMDKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAP 221
                 +E +   PE+S+  E   +CE MCS+GLRKYI +++SD   L  EIP ALK A 
Sbjct: 167 TVSQPSQEIV---PETSNKPEGGRMCELMCSKGLRKYIYANISDQAKLMEEIPSALKLAK 226

Query: 222 NPAQLVFDCIGRFYLQGRKAYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPS 281
            PA+ V DCIG+FYLQGR+A++K+SPM  ARQ S+LILE FL+          ++KIE  
Sbjct: 227 EPAKFVLDCIGKFYLQGRRAFTKESPMSSARQVSLLILESFLL---MPDRGKGKVKIESW 286

Query: 282 LKVEAHRAAIAWRKRIVNESGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNS 341
           +K EA  AA+AWRKR++ E G   A ++DARGLLL +A FG+P+ F + DL DL+R S S
Sbjct: 287 IKDEAETAAVAWRKRLMTEGGLAAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGS 346

Query: 342 KGISNALRRSHGLCTRIPDIIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECD 401
             I+ AL+RS  L   +  I++  +K+ M++EA++++Y FG+ED F    +L SFL+   
Sbjct: 347 NEIAGALKRSQFLVPMVSGIVESSIKRGMHIEALEMVYTFGMEDKFSAALVLTSFLKMSK 406

Query: 402 ETWKKRINEVRGSTMQLRRVSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLEN 461
           E++++   + + S +  +  + ++L  L  V++C+E HKLDP K LPGW+I E I +LE 
Sbjct: 407 ESFERAKRKAQ-SPLAFKEAATKQLAVLSSVMQCMETHKLDPAKELPGWQIKEQIVSLEK 456

Query: 462 DIVELGKRMEDNA-SMKRKTDEASTQKYPSQETKRSK 498
           D ++L K ME+ A S+    + A  ++  +Q+ KR +
Sbjct: 467 DTLQLDKEMEEKARSLSLMEEAALAKRMYNQQIKRPR 456

BLAST of Cp4.1LG10g12300 vs. ExPASy Swiss-Prot
Match: Q67Z93 (Inactive protein FRIGIDA OS=Arabidopsis thaliana OX=3702 GN=FRI PE=2 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 4.0e-50
Identity = 124/310 (40.00%), Postives = 176/310 (56.77%), Query Frame = 0

Query: 42  LGSESEPQETQFPFLNFSYIDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLIS 101
           +G   +PQ     FL    IDEL + S A+  F+ +  +LQ H+ SI NAID++      
Sbjct: 47  IGQSKQPQ-----FL--KSIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSK------ 106

Query: 102 SSNRLLDNHRQLLSVGDGKIALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSD 161
                L+++  +L+  +     P                      +L  PRNN+   ++ 
Sbjct: 107 -----LESNGVVLAARNNNFHQP----------------------MLSPPRNNVSVETTV 166

Query: 162 FGKNDGKEAMDKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAP 221
                 +E +   PE+S+  E E +CE MCS+GLRKYI +++SD   L  EIP ALK A 
Sbjct: 167 TVSQPSQEIV---PETSNKPEGERICELMCSKGLRKYIYANISDQAKLMEEIPSALKLAK 226

Query: 222 NPAQLVFDCIGRFYLQGRKAYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPS 281
            PA+ V DCIG+FYLQGR+A++K+SPM  ARQ S+LILE FL+          ++KIE  
Sbjct: 227 EPAKFVLDCIGKFYLQGRRAFTKESPMSSARQVSLLILESFLL---MPDRGKGKVKIESW 286

Query: 282 LKVEAHRAAIAWRKRIVNESGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNS 341
           +K EA  AA+AWRKR++ E G   A ++DARGLLL +A FG+P+ F + DL DL+R S S
Sbjct: 287 IKDEAETAAVAWRKRLMTEGGLAAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGS 310

Query: 342 KGISNALRRS 352
             I+ AL+RS
Sbjct: 347 NEIAGALKRS 310

BLAST of Cp4.1LG10g12300 vs. ExPASy Swiss-Prot
Match: Q67ZB3 (FRIGIDA-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=FRL3 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.9e-24
Identity = 99/383 (25.85%), Postives = 177/383 (46.21%), Query Frame = 0

Query: 135 KSGRNPVSSSTHLLRSPRNNLPETSSDFG----KNDGKEAMDKQPESS---------SLS 194
           K    PVS  +   R P+N + ++S+ F      +D  + + +  + S         +  
Sbjct: 114 KYNNAPVSKPSVGERWPQNAVEDSSNVFAADSITDDNPDGIVQDVQISPVMGNYEVKAYP 173

Query: 195 ELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFY-LQGRK 254
           +L  LC  M S GL K++  +  +L SL+ EIP+A + A NPA LV D +  FY ++   
Sbjct: 174 QLLKLCGDMDSTGLHKFVSDNRKNLASLKEEIPMAFRAAANPASLVLDSLEGFYPMEAPT 233

Query: 255 A-YSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVN 314
           A   KD+ ++  R++ ++++E   I  S    N   + +  ++K  A   A  W   + +
Sbjct: 234 ADGKKDANLLGMRRTCIMLMECLSILLSGLDRNCLAVVLSQNVKHRAKTIAEGWNPLLES 293

Query: 315 -ESGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRI 374
            +  +C  + ++A   L  LA+F I   F  ++L  L+   + +  +  L RS GL  ++
Sbjct: 294 LDMDACNGNSLEAHAFLQLLATFAIVADFKEDELLKLIPMVSRRRQAAELCRSLGLAEKM 353

Query: 375 PDIIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQL 434
           P +I+ +V     ++AV++ +AF L + F P  +L S+L E   +   +      S    
Sbjct: 354 PGVIEVLVNSGKQIDAVNLAFAFELTEQFSPVSLLKSYLIEARRS-SPQGRPGNASPAVQ 413

Query: 435 RRVSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKR 494
              +E +L  LK V+KC+E+H L+  +  P   +H+ I  LE                K 
Sbjct: 414 DEFNERELIGLKTVIKCIEEHSLE--EQYPVEPLHKRILQLE----------------KA 473

Query: 495 KTDEASTQKYPSQETKRSKGGFP 502
           K D+    +    + KR +G  P
Sbjct: 474 KADKKRATEPMKPQPKRPRGAQP 477

BLAST of Cp4.1LG10g12300 vs. ExPASy Swiss-Prot
Match: Q9LUV4 (FRIGIDA-like protein 4a OS=Arabidopsis thaliana OX=3702 GN=FRL4A PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.5e-20
Identity = 111/468 (23.72%), Postives = 193/468 (41.24%), Query Frame = 0

Query: 42  LGSESEPQE-TQFPFLNFSYIDELGSLSSAIHAFQCRL--SELQDHLGSIHNAIDARSKR 101
           +GS  +P E T+    +F    +  SL ++     C L   EL DH  S+   +  +S+ 
Sbjct: 1   MGSVPDPGELTELAQPSFEEFQKQTSLMTS-----CTLLWQELSDHFTSLEQNLMKKSEA 60

Query: 102 LISSSNRLLDNHRQLLSVGDGKIALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPET 161
           L       LDN  Q       +  +  + S  +   K G           R+   +L + 
Sbjct: 61  L-KQMIETLDNQTQTSLESLKRREVTIDHSVEIVAGKVGER--------ARAALESLEKA 120

Query: 162 SSDFGKNDGKEAMDKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALK 221
               G     ++ D   E   LS L+ LC  M +RG   ++ +   +L++LR +IP AL 
Sbjct: 121 RDGCGDGSNDDSGDVDDEEGLLSALKSLCLKMDARGFWNFVTARKKELENLRSKIPAALV 180

Query: 222 FAPNPAQLVFDCIGRFYLQGRKAYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKI 281
              +PA LV + I   +    +    D        + V+ILE              R+ +
Sbjct: 181 DCVDPAMLVLEAISEVFPVDTRG---DKVSNDYGWACVVILESLTPVIVDPVIGKSRLLV 240

Query: 282 EPSLKVEAHRAAIAWRKRIVNESGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRS 341
            PS+K +A   A  W+K +            D    L  L +FGI         R L+  
Sbjct: 241 TPSVKEKAKEIAETWKKSLEERGRIENVKTPDVHTFLQHLVTFGIVKSEDLALYRKLVVG 300

Query: 342 SNSKGISNALRRSHGLCTRIPDIIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQ 401
           S  +     L  S GL  ++PD+I+ ++ +   ++AV   Y  GL D FPP  +L ++L+
Sbjct: 301 SAWRKQMPKLAVSVGLGDQMPDMIEELISRGQQLDAVHFTYEVGLVDKFPPVPLLKAYLR 360

Query: 402 ECDETWKKRINEVRGSTMQLRRVSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKN 461
           +  ++    + +   +      V+ ++  +LK VLKC+E++KL+  +  P   + + +  
Sbjct: 361 DAKKSAASIMEDSSNTGRATHLVARKEQSALKAVLKCIEEYKLE--EEFPPENLKKRLDQ 420

Query: 462 LENDIVELGKRMEDNASMKRKTDEASTQKYPSQETKRSKGGFPVTSYP 507
           LE    E  KR        ++T  +     P  +  R    + V+S+P
Sbjct: 421 LEKTKTE--KRKPAAVPANKRTRASYNGPMPPAKAGRITNAY-VSSFP 446

BLAST of Cp4.1LG10g12300 vs. ExPASy Swiss-Prot
Match: Q940H8 (FRIGIDA-like protein 4b OS=Arabidopsis thaliana OX=3702 GN=FRL4B PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.0e-18
Identity = 99/436 (22.71%), Postives = 188/436 (43.12%), Query Frame = 0

Query: 80  ELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGKIALPYNSSSTVATLKSGRN 139
           EL +H  S+   +  +S+ L           RQ++   D +      + S++  LK    
Sbjct: 37  ELSEHFTSMEQNLMKKSEAL-----------RQMIETLDNQ------TQSSIELLKHREV 96

Query: 140 PVSSSTHL--------LRSPRNNLPETSSDFGKNDGKEAMDKQPESSSLSELEHLCETMC 199
            +  S  +        +R+  ++L E + D G  D  E  D       LS L+ LC  M 
Sbjct: 97  TIDHSVEIAEGKVEERVRAALDSL-EKARDCGDEDTGEVDD---GDGLLSALKSLCLKMD 156

Query: 200 SRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRKAYSKDSPMVHA 259
           +RG   ++++   +L++LR +IP+AL    +P +LV + +   +   ++    +      
Sbjct: 157 ARGFWGFVIARKKELENLRSQIPVALVDCVDPPKLVLEAVSEVFPVDKRG-GGEKVSNDF 216

Query: 260 RQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNESGSCKASEIDA 319
             + V+ILE  +           R+ + PS+K +A   A  W+  +    G       D 
Sbjct: 217 GWACVVILESLIPVMVDPVMGKSRLLVTPSVKEKAKEIAETWKASLEERGGIENVKTPDV 276

Query: 320 RGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPDIIKGMVKKSMN 379
              L  L +FGI         R L+  S  +     L  S GL  ++PD+I+ ++ +   
Sbjct: 277 HTFLQHLVTFGIVKKDDLALYRKLVVGSAWRKQMPKLAVSVGLGDQMPDMIEELIIRGQQ 336

Query: 380 VEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEKLGSLKC 439
           ++AV   +  GL  +FPP  +L ++L++  +      ++   S      V+ ++  +L+ 
Sbjct: 337 LDAVHFTFEVGLVHLFPPVPLLKAYLRDAKKATALITDDSNNSGRSAHLVARKEQSALRA 396

Query: 440 VLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKTDEASTQKYPSQ 499
           VLKC+E++KL+  +  P   + + +  LE    E  KR        ++T  + +   P  
Sbjct: 397 VLKCIEEYKLE--EEFPPENLKKRLDQLEKTKTE--KRKPAVIPANKRTRASYSGPMPPA 445

Query: 500 ETKRSKGGFPVTSYPP 508
           +  R    + V+S+PP
Sbjct: 457 KAGRITNAY-VSSFPP 445

BLAST of Cp4.1LG10g12300 vs. NCBI nr
Match: XP_023543896.1 (protein FRIGIDA [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1283 bits (3319), Expect = 0.0
Identity = 657/657 (100.00%), Postives = 657/657 (100.00%), Query Frame = 0

Query: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60
           MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY
Sbjct: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60

Query: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120
           IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120

Query: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180
           IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL
Sbjct: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180

Query: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240
           SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300
           AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE
Sbjct: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360
           SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD
Sbjct: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

Query: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420
           IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR
Sbjct: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420

Query: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480
           VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT
Sbjct: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480

Query: 481 DEASTQKYPSQETKRSKGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMSSSLHG 540
           DEASTQKYPSQETKRSKGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMSSSLHG
Sbjct: 481 DEASTQKYPSQETKRSKGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMSSSLHG 540

Query: 541 SSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLTTTPTP 600
           SSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLTTTPTP
Sbjct: 541 SSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLTTTPTP 600

Query: 601 PPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYFYN 657
           PPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYFYN
Sbjct: 601 PPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYFYN 657

BLAST of Cp4.1LG10g12300 vs. NCBI nr
Match: KAG6603163.1 (Protein FRIGIDA, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1241 bits (3210), Expect = 0.0
Identity = 639/662 (96.53%), Postives = 648/662 (97.89%), Query Frame = 0

Query: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60
           ME+DAMA AVDSL+EAVKVEEHSEASK+DAQLEKGSCQLPELGSESEPQETQFPFL FSY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQETQFPFLKFSY 60

Query: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120
           IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSN LLDNHRQLLSVGDGK
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180
           IALPYNSSS VATLKSGRNPVSSS+HLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL
Sbjct: 121 IALPYNSSSIVATLKSGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180

Query: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240
           SELEHLCETMCSRGLRKYIVSHLSD DSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDPDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300
           AYSKDSPMVHARQ+SVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360
           SGSCKAS+IDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

Query: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420
           IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQ CDETWKKRINEVRGSTMQLRR
Sbjct: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQLCDETWKKRINEVRGSTMQLRR 420

Query: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480
           VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT
Sbjct: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480

Query: 481 DEASTQKYPSQETKRS-----KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540
           DEASTQKYPSQETKRS     KGGFPV SYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS
Sbjct: 481 DEASTQKYPSQETKRSRTVESKGGFPVASYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540

Query: 541 SSLHGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLT 600
           SSL GSSLVETAVLPADMDISISNAGMD+SNEMGQTRELAFKD+SV QSFIQQAMPTTLT
Sbjct: 541 SSLRGSSLVETAVLPADMDISISNAGMDNSNEMGQTRELAFKDVSVGQSFIQQAMPTTLT 600

Query: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYF 657
           TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLEN+APKSSSTETSTLPHVRLSSHPRPPYF
Sbjct: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENEAPKSSSTETSTLPHVRLSSHPRPPYF 660

BLAST of Cp4.1LG10g12300 vs. NCBI nr
Match: KAG7033482.1 (Protein FRIGIDA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1241 bits (3210), Expect = 0.0
Identity = 639/662 (96.53%), Postives = 648/662 (97.89%), Query Frame = 0

Query: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60
           ME+DAMA AVDSL+EAVKVEEHSEASK+DAQLEKGSCQLPELGSESE QETQFPFL FSY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESESQETQFPFLKFSY 60

Query: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120
           IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSN LLDNHRQLLSVGDGK
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180
           IALPYNSSS VATLKSGRNPVSSS+HLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL
Sbjct: 121 IALPYNSSSIVATLKSGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180

Query: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240
           SELEHLCETMCSRGLRKYIVSHLSD DSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDPDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300
           AYSKDSPMVHARQ+SVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360
           SGSCKAS+IDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

Query: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420
           IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR
Sbjct: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420

Query: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480
           VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT
Sbjct: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480

Query: 481 DEASTQKYPSQETKRS-----KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540
           DEASTQKYPSQETKRS     KGGFPV SYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS
Sbjct: 481 DEASTQKYPSQETKRSRTVESKGGFPVASYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540

Query: 541 SSLHGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLT 600
           SSL GSSLVETAVLPADMDISISNAGMD+SNEMGQTRELAFKD+SV QSFIQQAMPTTLT
Sbjct: 541 SSLRGSSLVETAVLPADMDISISNAGMDNSNEMGQTRELAFKDVSVGQSFIQQAMPTTLT 600

Query: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYF 657
           TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLEN+APKSSSTETSTLPHVRLSSHPRPPYF
Sbjct: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENEAPKSSSTETSTLPHVRLSSHPRPPYF 660

BLAST of Cp4.1LG10g12300 vs. NCBI nr
Match: XP_022933109.1 (protein FRIGIDA [Cucurbita moschata])

HSP 1 Score: 1239 bits (3207), Expect = 0.0
Identity = 638/662 (96.37%), Postives = 645/662 (97.43%), Query Frame = 0

Query: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60
           ME+DAMA AVDSL+EAVKVEEHSEASK+DAQLEKGSCQLPELGSESEPQETQFPFL FSY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQETQFPFLKFSY 60

Query: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120
           IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSN LLDNHR LLSVGDGK
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRPLLSVGDGK 120

Query: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180
           IALPYNSSS VATLK GRNPVSSS+HLLRSPRNNLPETSSDFGKNDGKEAMDKQPESS L
Sbjct: 121 IALPYNSSSIVATLKRGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSPL 180

Query: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240
           SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300
           AYSKDSPMVHARQ+SVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360
           SGSCKAS+IDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

Query: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420
           IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR
Sbjct: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420

Query: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480
           VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT
Sbjct: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480

Query: 481 DEASTQKYPSQETKRS-----KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540
           DEASTQKYPSQETKRS     KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS
Sbjct: 481 DEASTQKYPSQETKRSRTVESKGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540

Query: 541 SSLHGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLT 600
           SSL GSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKD+SV QSFIQQ MPTTLT
Sbjct: 541 SSLRGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDVSVGQSFIQQPMPTTLT 600

Query: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYF 657
           TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLEND PKSSSTETSTLPHVRLSSHP PPYF
Sbjct: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDVPKSSSTETSTLPHVRLSSHPHPPYF 660

BLAST of Cp4.1LG10g12300 vs. NCBI nr
Match: XP_022967688.1 (protein FRIGIDA [Cucurbita maxima])

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 634/662 (95.77%), Postives = 643/662 (97.13%), Query Frame = 0

Query: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60
           ME+DAMA AVDSL+EAVKVEEHSEASK+DAQLEKGSCQLPELGSESEPQ+TQFPFL FSY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQKTQFPFLKFSY 60

Query: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120
           IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSN LLDNHRQLLSVGDGK
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180
           IALPYNSSS VATLKSGRNPVSSS+HLLRSPRNNLPETS DFGKNDGKEAMDKQPESSSL
Sbjct: 121 IALPYNSSSVVATLKSGRNPVSSSSHLLRSPRNNLPETSRDFGKNDGKEAMDKQPESSSL 180

Query: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240
           SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300
           AYSKDSPMVHARQ+SVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRI NE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIFNE 300

Query: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360
           SGSCKAS+IDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

Query: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420
           IIKGMVKKSMNVEAVDII+AFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR
Sbjct: 361 IIKGMVKKSMNVEAVDIIHAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420

Query: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480
           VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT
Sbjct: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480

Query: 481 DEASTQKYPSQETKRS-----KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540
           DEASTQKYPSQETKRS     KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS
Sbjct: 481 DEASTQKYPSQETKRSRTVESKGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540

Query: 541 SSLHGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLT 600
            SL GSSLVET VLPADMDISISNAGMDSSNEMGQTRELAFKD+SV QSFIQQAMPTTL 
Sbjct: 541 LSLSGSSLVETGVLPADMDISISNAGMDSSNEMGQTRELAFKDVSVGQSFIQQAMPTTLA 600

Query: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYF 657
           TTPT  PPVVSHSAVEGGFVDLYHFGDAVVLEND PKSSSTETSTLPHVRLSSHPRPPYF
Sbjct: 601 TTPTLAPPVVSHSAVEGGFVDLYHFGDAVVLENDVPKSSSTETSTLPHVRLSSHPRPPYF 660

BLAST of Cp4.1LG10g12300 vs. ExPASy TrEMBL
Match: A0A6J1EYU0 (FRIGIDA-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439873 PE=3 SV=1)

HSP 1 Score: 1239 bits (3207), Expect = 0.0
Identity = 638/662 (96.37%), Postives = 645/662 (97.43%), Query Frame = 0

Query: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60
           ME+DAMA AVDSL+EAVKVEEHSEASK+DAQLEKGSCQLPELGSESEPQETQFPFL FSY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQETQFPFLKFSY 60

Query: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120
           IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSN LLDNHR LLSVGDGK
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRPLLSVGDGK 120

Query: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180
           IALPYNSSS VATLK GRNPVSSS+HLLRSPRNNLPETSSDFGKNDGKEAMDKQPESS L
Sbjct: 121 IALPYNSSSIVATLKRGRNPVSSSSHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSPL 180

Query: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240
           SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300
           AYSKDSPMVHARQ+SVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300

Query: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360
           SGSCKAS+IDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

Query: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420
           IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR
Sbjct: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420

Query: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480
           VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT
Sbjct: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480

Query: 481 DEASTQKYPSQETKRS-----KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540
           DEASTQKYPSQETKRS     KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS
Sbjct: 481 DEASTQKYPSQETKRSRTVESKGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540

Query: 541 SSLHGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLT 600
           SSL GSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKD+SV QSFIQQ MPTTLT
Sbjct: 541 SSLRGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDVSVGQSFIQQPMPTTLT 600

Query: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYF 657
           TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLEND PKSSSTETSTLPHVRLSSHP PPYF
Sbjct: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDVPKSSSTETSTLPHVRLSSHPHPPYF 660

BLAST of Cp4.1LG10g12300 vs. ExPASy TrEMBL
Match: A0A6J1HVU4 (FRIGIDA-like protein OS=Cucurbita maxima OX=3661 GN=LOC111467142 PE=3 SV=1)

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 634/662 (95.77%), Postives = 643/662 (97.13%), Query Frame = 0

Query: 1   MESDAMAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSY 60
           ME+DAMA AVDSL+EAVKVEEHSEASK+DAQLEKGSCQLPELGSESEPQ+TQFPFL FSY
Sbjct: 1   MENDAMAAAVDSLMEAVKVEEHSEASKTDAQLEKGSCQLPELGSESEPQKTQFPFLKFSY 60

Query: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGK 120
           IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSN LLDNHRQLLSVGDGK
Sbjct: 61  IDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNCLLDNHRQLLSVGDGK 120

Query: 121 IALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSL 180
           IALPYNSSS VATLKSGRNPVSSS+HLLRSPRNNLPETS DFGKNDGKEAMDKQPESSSL
Sbjct: 121 IALPYNSSSVVATLKSGRNPVSSSSHLLRSPRNNLPETSRDFGKNDGKEAMDKQPESSSL 180

Query: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240
           SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK
Sbjct: 181 SELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRK 240

Query: 241 AYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNE 300
           AYSKDSPMVHARQ+SVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRI NE
Sbjct: 241 AYSKDSPMVHARQASVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIFNE 300

Query: 301 SGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360
           SGSCKAS+IDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD
Sbjct: 301 SGSCKASDIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPD 360

Query: 361 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420
           IIKGMVKKSMNVEAVDII+AFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR
Sbjct: 361 IIKGMVKKSMNVEAVDIIHAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 420

Query: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480
           VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT
Sbjct: 421 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 480

Query: 481 DEASTQKYPSQETKRS-----KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540
           DEASTQKYPSQETKRS     KGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS
Sbjct: 481 DEASTQKYPSQETKRSRTVESKGGFPVTSYPPVNGLLEQNAAATLFDGAGVDQFGNYQMS 540

Query: 541 SSLHGSSLVETAVLPADMDISISNAGMDSSNEMGQTRELAFKDMSVEQSFIQQAMPTTLT 600
            SL GSSLVET VLPADMDISISNAGMDSSNEMGQTRELAFKD+SV QSFIQQAMPTTL 
Sbjct: 541 LSLSGSSLVETGVLPADMDISISNAGMDSSNEMGQTRELAFKDVSVGQSFIQQAMPTTLA 600

Query: 601 TTPTPPPPVVSHSAVEGGFVDLYHFGDAVVLENDAPKSSSTETSTLPHVRLSSHPRPPYF 657
           TTPT  PPVVSHSAVEGGFVDLYHFGDAVVLEND PKSSSTETSTLPHVRLSSHPRPPYF
Sbjct: 601 TTPTLAPPVVSHSAVEGGFVDLYHFGDAVVLENDVPKSSSTETSTLPHVRLSSHPRPPYF 660

BLAST of Cp4.1LG10g12300 vs. ExPASy TrEMBL
Match: A0A5D3DV89 (FRIGIDA-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold325G001270 PE=3 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 4.92e-315
Identity = 487/695 (70.07%), Postives = 540/695 (77.70%), Query Frame = 0

Query: 6   MAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSYIDELG 65
           MA  VDS     K EEHSEA    ++L +G CQL ELGSES PQ+  F  L  SY DELG
Sbjct: 1   MAATVDSF----KDEEHSEAV---SRLVEGPCQLRELGSESNPQQPPFKLLKNSYFDELG 60

Query: 66  SLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGKIALPY 125
           SLS+AIHAFQCR +ELQDHL  IHNAIDARSK+ +SSSN LLDNH QLLS      +L +
Sbjct: 61  SLSTAIHAFQCRFNELQDHLCFIHNAIDARSKQHMSSSNSLLDNHSQLLS------SLSF 120

Query: 126 NSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSLSELEH 185
            SS+ V T +SGRNPV SSTH  RSPR+NLPET +  GK DGKE +DKQ ESSSLSELEH
Sbjct: 121 KSSTLVPTAESGRNPVYSSTHPSRSPRSNLPETRTYSGKVDGKEGVDKQTESSSLSELEH 180

Query: 186 LCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRKAYSKD 245
           LC+TMCSRGLRKYIVSHLSDL +LRHEIPLALK APNPA+LVFDCIGRFYLQG KAY+KD
Sbjct: 181 LCQTMCSRGLRKYIVSHLSDLANLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYTKD 240

Query: 246 SPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNESGSCK 305
           SPM+ ARQ+S+LILELFLISG+AET+ND+R +IE SLKVEA  AAIAWRKR++ ESGSC+
Sbjct: 241 SPMIPARQASILILELFLISGAAETKNDKRTEIESSLKVEADLAAIAWRKRLITESGSCQ 300

Query: 306 ASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPDIIKGM 365
           AS+IDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKG+SNAL  SH L TRIPDIIKGM
Sbjct: 301 ASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGLSNALCHSHCLRTRIPDIIKGM 360

Query: 366 VKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEK 425
            K S N+EAVDIIYAFG+EDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEK
Sbjct: 361 TKSSKNIEAVDIIYAFGMEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEK 420

Query: 426 LGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKTDEAST 485
           L SLKCVLKCLEDHKLDPVKSLPGW+IHEMIKNLE DIVEL KRMEDNAS+KRKTDEAST
Sbjct: 421 LASLKCVLKCLEDHKLDPVKSLPGWKIHEMIKNLEKDIVELRKRMEDNASLKRKTDEAST 480

Query: 486 QKYPSQETKRS----KGGFPVTSYPPVNGLLEQNAAA-----------------TLFDGA 545
           QKY SQE KRS    KGGFPV SYP VNGLLEQNAA                   L DG 
Sbjct: 481 QKYLSQEIKRSRLASKGGFPVMSYP-VNGLLEQNAATFLEDKSCFSTSSSSMPQKLLDGG 540

Query: 546 GVDQFGNYQMSSSLHGSSLVETAVLPADMDISISNA----------GMDSSN----EMGQ 605
              Q GNYQ++SSL G  L+ET VLPAD+   I+NA          G DS+     +MG 
Sbjct: 541 RASQLGNYQIASSLRGPGLLETTVLPADIGSGITNAAASFLRGMGRGRDSNEASIYKMGP 600

Query: 606 TRELAFKDMSVEQSFIQQAMPTTLTTTPTPPPPVVSHSAVEGGFV--------DLYHFGD 657
           TRELA+KD+SV QSFIQQAMP TL TTPTPPP  V   +   GF+        DLYHF D
Sbjct: 601 TRELAYKDISVGQSFIQQAMP-TLATTPTPPPTTVEPYSAVDGFMGHSTSNNFDLYHFAD 660

BLAST of Cp4.1LG10g12300 vs. ExPASy TrEMBL
Match: A0A1S3BM50 (FRIGIDA-like protein OS=Cucumis melo OX=3656 GN=LOC103491359 PE=3 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 4.92e-315
Identity = 487/695 (70.07%), Postives = 540/695 (77.70%), Query Frame = 0

Query: 6   MAPAVDSLVEAVKVEEHSEASKSDAQLEKGSCQLPELGSESEPQETQFPFLNFSYIDELG 65
           MA  VDS     K EEHSEA    ++L +G CQL ELGSES PQ+  F  L  SY DELG
Sbjct: 1   MAATVDSF----KDEEHSEAV---SRLVEGPCQLRELGSESNPQQPPFKLLKNSYFDELG 60

Query: 66  SLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGKIALPY 125
           SLS+AIHAFQCR +ELQDHL  IHNAIDARSK+ +SSSN LLDNH QLLS      +L +
Sbjct: 61  SLSTAIHAFQCRFNELQDHLCFIHNAIDARSKQHMSSSNSLLDNHSQLLS------SLSF 120

Query: 126 NSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSLSELEH 185
            SS+ V T +SGRNPV SSTH  RSPR+NLPET +  GK DGKE +DKQ ESSSLSELEH
Sbjct: 121 KSSTLVPTAESGRNPVYSSTHPSRSPRSNLPETRTYSGKVDGKEGVDKQTESSSLSELEH 180

Query: 186 LCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRKAYSKD 245
           LC+TMCSRGLRKYIVSHLSDL +LRHEIPLALK APNPA+LVFDCIGRFYLQG KAY+KD
Sbjct: 181 LCQTMCSRGLRKYIVSHLSDLANLRHEIPLALKCAPNPAKLVFDCIGRFYLQGSKAYTKD 240

Query: 246 SPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNESGSCK 305
           SPM+ ARQ+S+LILELFLISG+AET+ND+R +IE SLKVEA  AAIAWRKR++ ESGSC+
Sbjct: 241 SPMIPARQASILILELFLISGAAETKNDKRTEIESSLKVEADLAAIAWRKRLITESGSCQ 300

Query: 306 ASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPDIIKGM 365
           AS+IDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKG+SNAL  SH L TRIPDIIKGM
Sbjct: 301 ASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGLSNALCHSHCLRTRIPDIIKGM 360

Query: 366 VKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEK 425
            K S N+EAVDIIYAFG+EDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEK
Sbjct: 361 TKSSKNIEAVDIIYAFGMEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEK 420

Query: 426 LGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKTDEAST 485
           L SLKCVLKCLEDHKLDPVKSLPGW+IHEMIKNLE DIVEL KRMEDNAS+KRKTDEAST
Sbjct: 421 LASLKCVLKCLEDHKLDPVKSLPGWKIHEMIKNLEKDIVELRKRMEDNASLKRKTDEAST 480

Query: 486 QKYPSQETKRS----KGGFPVTSYPPVNGLLEQNAAA-----------------TLFDGA 545
           QKY SQE KRS    KGGFPV SYP VNGLLEQNAA                   L DG 
Sbjct: 481 QKYLSQEIKRSRLASKGGFPVMSYP-VNGLLEQNAATFLEDKSCFSTSSSSMPQKLLDGG 540

Query: 546 GVDQFGNYQMSSSLHGSSLVETAVLPADMDISISNA----------GMDSSN----EMGQ 605
              Q GNYQ++SSL G  L+ET VLPAD+   I+NA          G DS+     +MG 
Sbjct: 541 RASQLGNYQIASSLRGPGLLETTVLPADIGSGITNAAASFPRGMGRGRDSNEASIYKMGP 600

Query: 606 TRELAFKDMSVEQSFIQQAMPTTLTTTPTPPPPVVSHSAVEGGFV--------DLYHFGD 657
           TRELA+KD+SV QSFIQQAMP TL TTPTPPP  V   +   GF+        DLYHF D
Sbjct: 601 TRELAYKDISVGQSFIQQAMP-TLATTPTPPPTTVEPYSAVDGFMGHSTSNNFDLYHFAD 660

BLAST of Cp4.1LG10g12300 vs. ExPASy TrEMBL
Match: A0A0A0KYX9 (FRIGIDA-like protein OS=Cucumis sativus OX=3659 GN=Csa_4G437530 PE=3 SV=1)

HSP 1 Score: 758 bits (1956), Expect = 5.97e-268
Identity = 415/579 (71.68%), Postives = 454/579 (78.41%), Query Frame = 0

Query: 126 NSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSDFGKNDGKEAMDKQPESSSLSELEH 185
           NSS  V T +SGRNPV SSTHL RS R+NLPET +DFGK DGKE +DKQPESSSLS+LEH
Sbjct: 36  NSSPLVPTAESGRNPVYSSTHLSRSSRSNLPETRTDFGKVDGKEGVDKQPESSSLSQLEH 95

Query: 186 LCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRKAYSKD 245
           LC TMCSRGLRKYIVSHLSDL  L HEIPLALK+APNPA+LVFDCIGRFYLQG KAY+K 
Sbjct: 96  LCRTMCSRGLRKYIVSHLSDLARLHHEIPLALKWAPNPAKLVFDCIGRFYLQGSKAYTKV 155

Query: 246 SPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNESGSCK 305
           SPM+ ARQ+S+LILELFLISG+AET+ND+R +IE SLKVEA  AAIAWRKR++ ESGSC+
Sbjct: 156 SPMIPARQASILILELFLISGAAETKNDKRTEIESSLKVEADLAAIAWRKRLITESGSCQ 215

Query: 306 ASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPDIIKGM 365
           AS+IDARGLLLFLASFGIPTVFTN+DLRDLLRSSNSKG+SNAL  SH L TRIPDIIKGM
Sbjct: 216 ASDIDARGLLLFLASFGIPTVFTNDDLRDLLRSSNSKGLSNALCHSHCLRTRIPDIIKGM 275

Query: 366 VKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEK 425
            K S N+EAVDIIYAFG+E+VFPPQEILLSFLQECDETWKKRIN+VRGSTMQLRRVSEEK
Sbjct: 276 TKSSKNIEAVDIIYAFGMENVFPPQEILLSFLQECDETWKKRINKVRGSTMQLRRVSEEK 335

Query: 426 LGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKTDEAST 485
           L SLKCVLKCLEDHKLDPVKSLPGW+IHEMIKNLE DIVELGKRMEDNAS+KRKTDEAST
Sbjct: 336 LASLKCVLKCLEDHKLDPVKSLPGWKIHEMIKNLEKDIVELGKRMEDNASLKRKTDEAST 395

Query: 486 QKYPSQETKRS-----KGGFPVTSYPPVNGLLEQNAAA-----------------TLFDG 545
           QKY SQE KRS     KGGFPV SYP VNGLLEQNA                    L DG
Sbjct: 396 QKYLSQEIKRSRMSANKGGFPVMSYP-VNGLLEQNATTFLEDKSCFSTSSSSMPLKLLDG 455

Query: 546 AGVDQFGNYQMSSSLHGSSLVETAVLPADMDIS-ISNA------------GMDSSN---- 605
               Q GNYQ++SSL G  LVET VLPAD+  S ISNA            G DS+     
Sbjct: 456 GRASQLGNYQIASSLRGPGLVETTVLPADIIGSGISNAAAPFPRGMGWGRGRDSNEASIY 515

Query: 606 EMGQTRELAFKDMSVEQSFIQQAMPTTLTTTPTPPPPVVSHSAVEGGFV--------DLY 657
           +MG TRE A+KD+SV QSFIQQAMP TL TTPTPPP  V   +   GF+        DLY
Sbjct: 516 KMGPTREFAYKDISVGQSFIQQAMP-TLATTPTPPPTTVEPYSAVYGFMGHSTSNNFDLY 575

BLAST of Cp4.1LG10g12300 vs. TAIR 10
Match: AT4G00650.1 (FRIGIDA-like protein )

HSP 1 Score: 201.1 bits (510), Expect = 2.8e-51
Identity = 124/310 (40.00%), Postives = 176/310 (56.77%), Query Frame = 0

Query: 42  LGSESEPQETQFPFLNFSYIDELGSLSSAIHAFQCRLSELQDHLGSIHNAIDARSKRLIS 101
           +G   +PQ     FL    IDEL + S A+  F+ +  +LQ H+ SI NAID++      
Sbjct: 47  IGQSKQPQ-----FL--KSIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSK------ 106

Query: 102 SSNRLLDNHRQLLSVGDGKIALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPETSSD 161
                L+++  +L+  +     P                      +L  PRNN+   ++ 
Sbjct: 107 -----LESNGVVLAARNNNFHQP----------------------MLSPPRNNVSVETTV 166

Query: 162 FGKNDGKEAMDKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAP 221
                 +E +   PE+S+  E E +CE MCS+GLRKYI +++SD   L  EIP ALK A 
Sbjct: 167 TVSQPSQEIV---PETSNKPEGERICELMCSKGLRKYIYANISDQAKLMEEIPSALKLAK 226

Query: 222 NPAQLVFDCIGRFYLQGRKAYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPS 281
            PA+ V DCIG+FYLQGR+A++K+SPM  ARQ S+LILE FL+          ++KIE  
Sbjct: 227 EPAKFVLDCIGKFYLQGRRAFTKESPMSSARQVSLLILESFLL---MPDRGKGKVKIESW 286

Query: 282 LKVEAHRAAIAWRKRIVNESGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNS 341
           +K EA  AA+AWRKR++ E G   A ++DARGLLL +A FG+P+ F + DL DL+R S S
Sbjct: 287 IKDEAETAAVAWRKRLMTEGGLAAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGS 310

Query: 342 KGISNALRRS 352
             I+ AL+RS
Sbjct: 347 NEIAGALKRS 310

BLAST of Cp4.1LG10g12300 vs. TAIR 10
Match: AT5G48385.1 (FRIGIDA-like protein )

HSP 1 Score: 114.4 bits (285), Expect = 3.5e-25
Identity = 99/383 (25.85%), Postives = 177/383 (46.21%), Query Frame = 0

Query: 135 KSGRNPVSSSTHLLRSPRNNLPETSSDFG----KNDGKEAMDKQPESS---------SLS 194
           K    PVS  +   R P+N + ++S+ F      +D  + + +  + S         +  
Sbjct: 114 KYNNAPVSKPSVGERWPQNAVEDSSNVFAADSITDDNPDGIVQDVQISPVMGNYEVKAYP 173

Query: 195 ELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFY-LQGRK 254
           +L  LC  M S GL K++  +  +L SL+ EIP+A + A NPA LV D +  FY ++   
Sbjct: 174 QLLKLCGDMDSTGLHKFVSDNRKNLASLKEEIPMAFRAAANPASLVLDSLEGFYPMEAPT 233

Query: 255 A-YSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVN 314
           A   KD+ ++  R++ ++++E   I  S    N   + +  ++K  A   A  W   + +
Sbjct: 234 ADGKKDANLLGMRRTCIMLMECLSILLSGLDRNCLAVVLSQNVKHRAKTIAEGWNPLLES 293

Query: 315 -ESGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRI 374
            +  +C  + ++A   L  LA+F I   F  ++L  L+   + +  +  L RS GL  ++
Sbjct: 294 LDMDACNGNSLEAHAFLQLLATFAIVADFKEDELLKLIPMVSRRRQAAELCRSLGLAEKM 353

Query: 375 PDIIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQL 434
           P +I+ +V     ++AV++ +AF L + F P  +L S+L E   +   +      S    
Sbjct: 354 PGVIEVLVNSGKQIDAVNLAFAFELTEQFSPVSLLKSYLIEARRS-SPQGRPGNASPAVQ 413

Query: 435 RRVSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKR 494
              +E +L  LK V+KC+E+H L+  +  P   +H+ I  LE                K 
Sbjct: 414 DEFNERELIGLKTVIKCIEEHSLE--EQYPVEPLHKRILQLE----------------KA 473

Query: 495 KTDEASTQKYPSQETKRSKGGFP 502
           K D+    +    + KR +G  P
Sbjct: 474 KADKKRATEPMKPQPKRPRGAQP 477

BLAST of Cp4.1LG10g12300 vs. TAIR 10
Match: AT3G22440.1 (FRIGIDA-like protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.8e-21
Identity = 111/468 (23.72%), Postives = 193/468 (41.24%), Query Frame = 0

Query: 42  LGSESEPQE-TQFPFLNFSYIDELGSLSSAIHAFQCRL--SELQDHLGSIHNAIDARSKR 101
           +GS  +P E T+    +F    +  SL ++     C L   EL DH  S+   +  +S+ 
Sbjct: 1   MGSVPDPGELTELAQPSFEEFQKQTSLMTS-----CTLLWQELSDHFTSLEQNLMKKSEA 60

Query: 102 LISSSNRLLDNHRQLLSVGDGKIALPYNSSSTVATLKSGRNPVSSSTHLLRSPRNNLPET 161
           L       LDN  Q       +  +  + S  +   K G           R+   +L + 
Sbjct: 61  L-KQMIETLDNQTQTSLESLKRREVTIDHSVEIVAGKVGER--------ARAALESLEKA 120

Query: 162 SSDFGKNDGKEAMDKQPESSSLSELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALK 221
               G     ++ D   E   LS L+ LC  M +RG   ++ +   +L++LR +IP AL 
Sbjct: 121 RDGCGDGSNDDSGDVDDEEGLLSALKSLCLKMDARGFWNFVTARKKELENLRSKIPAALV 180

Query: 222 FAPNPAQLVFDCIGRFYLQGRKAYSKDSPMVHARQSSVLILELFLISGSAETENDRRIKI 281
              +PA LV + I   +    +    D        + V+ILE              R+ +
Sbjct: 181 DCVDPAMLVLEAISEVFPVDTRG---DKVSNDYGWACVVILESLTPVIVDPVIGKSRLLV 240

Query: 282 EPSLKVEAHRAAIAWRKRIVNESGSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRS 341
            PS+K +A   A  W+K +            D    L  L +FGI         R L+  
Sbjct: 241 TPSVKEKAKEIAETWKKSLEERGRIENVKTPDVHTFLQHLVTFGIVKSEDLALYRKLVVG 300

Query: 342 SNSKGISNALRRSHGLCTRIPDIIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQ 401
           S  +     L  S GL  ++PD+I+ ++ +   ++AV   Y  GL D FPP  +L ++L+
Sbjct: 301 SAWRKQMPKLAVSVGLGDQMPDMIEELISRGQQLDAVHFTYEVGLVDKFPPVPLLKAYLR 360

Query: 402 ECDETWKKRINEVRGSTMQLRRVSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKN 461
           +  ++    + +   +      V+ ++  +LK VLKC+E++KL+  +  P   + + +  
Sbjct: 361 DAKKSAASIMEDSSNTGRATHLVARKEQSALKAVLKCIEEYKLE--EEFPPENLKKRLDQ 420

Query: 462 LENDIVELGKRMEDNASMKRKTDEASTQKYPSQETKRSKGGFPVTSYP 507
           LE    E  KR        ++T  +     P  +  R    + V+S+P
Sbjct: 421 LEKTKTE--KRKPAAVPANKRTRASYNGPMPPAKAGRITNAY-VSSFP 446

BLAST of Cp4.1LG10g12300 vs. TAIR 10
Match: AT4G14900.1 (FRIGIDA-like protein )

HSP 1 Score: 94.7 bits (234), Expect = 2.9e-19
Identity = 99/436 (22.71%), Postives = 188/436 (43.12%), Query Frame = 0

Query: 80  ELQDHLGSIHNAIDARSKRLISSSNRLLDNHRQLLSVGDGKIALPYNSSSTVATLKSGRN 139
           EL +H  S+   +  +S+ L           RQ++   D +      + S++  LK    
Sbjct: 37  ELSEHFTSMEQNLMKKSEAL-----------RQMIETLDNQ------TQSSIELLKHREV 96

Query: 140 PVSSSTHL--------LRSPRNNLPETSSDFGKNDGKEAMDKQPESSSLSELEHLCETMC 199
            +  S  +        +R+  ++L E + D G  D  E  D       LS L+ LC  M 
Sbjct: 97  TIDHSVEIAEGKVEERVRAALDSL-EKARDCGDEDTGEVDD---GDGLLSALKSLCLKMD 156

Query: 200 SRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRKAYSKDSPMVHA 259
           +RG   ++++   +L++LR +IP+AL    +P +LV + +   +   ++    +      
Sbjct: 157 ARGFWGFVIARKKELENLRSQIPVALVDCVDPPKLVLEAVSEVFPVDKRG-GGEKVSNDF 216

Query: 260 RQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNESGSCKASEIDA 319
             + V+ILE  +           R+ + PS+K +A   A  W+  +    G       D 
Sbjct: 217 GWACVVILESLIPVMVDPVMGKSRLLVTPSVKEKAKEIAETWKASLEERGGIENVKTPDV 276

Query: 320 RGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGLCTRIPDIIKGMVKKSMN 379
              L  L +FGI         R L+  S  +     L  S GL  ++PD+I+ ++ +   
Sbjct: 277 HTFLQHLVTFGIVKKDDLALYRKLVVGSAWRKQMPKLAVSVGLGDQMPDMIEELIIRGQQ 336

Query: 380 VEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRRVSEEKLGSLKC 439
           ++AV   +  GL  +FPP  +L ++L++  +      ++   S      V+ ++  +L+ 
Sbjct: 337 LDAVHFTFEVGLVHLFPPVPLLKAYLRDAKKATALITDDSNNSGRSAHLVARKEQSALRA 396

Query: 440 VLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKTDEASTQKYPSQ 499
           VLKC+E++KL+  +  P   + + +  LE    E  KR        ++T  + +   P  
Sbjct: 397 VLKCIEEYKLE--EEFPPENLKKRLDQLEKTKTE--KRKPAVIPANKRTRASYSGPMPPA 445

Query: 500 ETKRSKGGFPVTSYPP 508
           +  R    + V+S+PP
Sbjct: 457 KAGRITNAY-VSSFPP 445

BLAST of Cp4.1LG10g12300 vs. TAIR 10
Match: AT1G31814.1 (FRIGIDA like 2 )

HSP 1 Score: 74.7 bits (182), Expect = 3.1e-13
Identity = 69/311 (22.19%), Postives = 135/311 (43.41%), Query Frame = 0

Query: 182 ELEHLCETMCSRGLRKYIVSHLSDLDSLRHEIPLALKFAPNPAQLVFDCIGRFYLQGRKA 241
           EL   CE    +GL  Y++ +     S+  E+P A++ + NPA LV D I   Y     +
Sbjct: 89  ELRKFCEKNDGKGLGNYMIENSRKRLSINEELPNAIRCSENPAPLVLDAIEGSYHCSSPS 148

Query: 242 YSKDSPMVHARQSSVLILELFLISGSAETENDRRIKIEPSLKVEAHRAAIAWRKRIVNES 301
            S  +  +  ++  VL+LE  LI  +A   ND        L+  A   A  W+  I N+ 
Sbjct: 149 SSSSARAIDVKRIFVLLLEA-LIEINANLTND--------LRERARTIAYDWKPNIGNKP 208

Query: 302 GSCKASEIDARGLLLFLASFGIPTVFTNNDLRDLLRSSNSKGISNALRRSHGL-CTRIPD 361
                   +A G L  +A+F + ++F+  ++ D +   +    +  + +  GL   RI  
Sbjct: 209 S-------EALGFLHLVAAFELGSLFSTEEICDYIFLISKYKQATTICKKIGLDRNRIGV 268

Query: 362 IIKGMVKKSMNVEAVDIIYAFGLEDVFPPQEILLSFLQECDETWKKRINEVRGSTMQLRR 421
           +++  +     + A+  IY   +   F P  IL + L+   E  K+   E   S      
Sbjct: 269 LVQKFLDTGRLLVAIRFIYENEMVGEFEPVSILKTSLKNSREAAKRVCAEGNYSLKVQNE 328

Query: 422 VSEEKLGSLKCVLKCLEDHKLDPVKSLPGWEIHEMIKNLENDIVELGKRMEDNASMKRKT 481
            ++++L +L+ V+K +++  ++              + +E  + E  K +ED  + +++ 
Sbjct: 329 ATDKELSALRAVIKVVKEKNIES-------------EFMEEKLEECVKELEDQKAQRKRA 370

Query: 482 DEASTQKYPSQ 492
            + ++   P Q
Sbjct: 389 TKFNSPANPQQ 370