Cp4.1LG10g09860 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG10g09860 vs. ExPASy Swiss-Prot
Match: O04057 (Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 4.6e-185
Identity = 316/483 (65.42%), Postives = 389/483 (80.54%), Query Frame = 0

Query: 32  LLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGSKHDQW--VNDVG 91
           L L++  +  SSASN+GL+R+GLKKIK++    L A VESK  + L +   ++    ++G
Sbjct: 13  LFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKGNLG 72

Query: 92  ESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIACFFHAR 151
           ES ++DIVALKNY+DAQYYGEI IGTPPQKFTVIFDTGSSNLWV   +C+FS+AC FHAR
Sbjct: 73  ESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV-LCECLFSVACHFHAR 132

Query: 152 YQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSMTFIAA 211
           Y+S RSS+Y++NGTSA+I+YGTGA+SGFFSYDNV+VGD+VV++Q FIE T   S+TF+ A
Sbjct: 133 YKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTFLVA 192

Query: 212 KFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFGGVDPK 271
           KFDG+LGLGFQEI+ G+AVPVWYNMV Q LVKEPVFSFWLNRN +EEEGGEIVFGGVDPK
Sbjct: 193 KFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGGVDPK 252

Query: 272 HFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL------------ 331
           H++G+HTYVPVT KGYWQF++GD+LI GEPT +C  GCSAIADSGTSL            
Sbjct: 253 HYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVITMIN 312

Query: 332 -AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMKIESVA 391
            AIGA G+   +CKAVV+Q+G++IMDLLL++  P+KICS+I +C FDGT GVSM IESV 
Sbjct: 313 HAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIESVV 372

Query: 392 NEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQGETLVD 451
           +E  G+SS    D MCS CEM V WM ++L+QN+T+E +I+Y+N+LCDR  S  G++ VD
Sbjct: 373 DENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQSAVD 432

Query: 452 CGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLWILGDV 499
           CG++S MPTVSFTIG K+F+L  E+YILKVGEG  AQCISGF   DIPPPRGPLWILGDV
Sbjct: 433 CGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWILGDV 492

BLAST of Cp4.1LG10g09860 vs. ExPASy Swiss-Prot
Match: Q8VYL3 (Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 2.6e-180
Identity = 304/484 (62.81%), Postives = 383/484 (79.13%), Query Frame = 0

Query: 30  SLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGSKHDQWVNDV- 89
           S+ +  L   TA S  N+G  R+GLKK+K++ N  L     SK+ + L S    + N++ 
Sbjct: 11  SVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYNNNLG 70

Query: 90  GESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIACFFHA 149
           G+S ++DIV LKNY+DAQYYGEI IGTPPQKFTVIFDTGSSNLWVPS KC FS++C+FHA
Sbjct: 71  GDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFSLSCYFHA 130

Query: 150 RYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSMTFIA 209
           +Y+S RSSTY+++G  AAI YG+G+ISGFFSYD V VGD+VV+DQ+FIETTS   +TF+ 
Sbjct: 131 KYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSEPGLTFLV 190

Query: 210 AKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFGGVDP 269
           AKFDG+LGLGFQEI+ G+A PVWYNM+ Q L+K PVFSFWLNR+ K EEGGEIVFGGVDP
Sbjct: 191 AKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEIVFGGVDP 250

Query: 270 KHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL----------- 329
           KHF+G+HT+VPVT +GYWQF++G++LI GE T YC  GCSAIADSGTSL           
Sbjct: 251 KHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGPTAVVAMI 310

Query: 330 --AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMKIESV 389
             AIGA+G+   +CK VV Q+G++I+DLLLA+ QP+KICS+IG+CA+DGTHGVSM IESV
Sbjct: 311 NKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGVSMGIESV 370

Query: 390 ANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQGETLV 449
            ++++ RSS G  DA C ACEMAV W+  +L+QN TQE +++Y+N++C+R  S  GE+ V
Sbjct: 371 VDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAV 430

Query: 450 DCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLWILGD 499
           DC ++S+MPTVSFTIG KVF+L  E+Y+LK+GEG  AQCISGF  LDIPPPRGPLWILGD
Sbjct: 431 DCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGD 490

BLAST of Cp4.1LG10g09860 vs. ExPASy Swiss-Prot
Match: O65390 (Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 7.6e-180
Identity = 308/488 (63.11%), Postives = 378/488 (77.46%), Query Frame = 0

Query: 25  KPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGSKHDQW 84
           + + VSL++  L   +A +  N+G  R+GLKK+K++    L A VESK+ K L +     
Sbjct: 6   RTVAVSLIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRAYR--- 65

Query: 85  VNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIAC 144
              +G+S ++D+V LKNY+DAQYYGEI IGTPPQKFTV+FDTGSSNLWVPSSKC FS+AC
Sbjct: 66  ---LGDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLAC 125

Query: 145 FFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSM 204
             H +Y+S RSSTY +NG +AAI YGTGAI+GFFS D V VGD+VV+DQ+FIE T    +
Sbjct: 126 LLHPKYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGI 185

Query: 205 TFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFG 264
           TF+ AKFDGILGLGFQEIS G A PVWYNM+ Q L+KEPVFSFWLNRNA EEEGGE+VFG
Sbjct: 186 TFVVAKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELVFG 245

Query: 265 GVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL------- 324
           GVDP HFKG+HTYVPVT KGYWQF++GD+LIGG PT +C  GCSAIADSGTSL       
Sbjct: 246 GVDPNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTI 305

Query: 325 ------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMK 384
                 AIGAAG+   +CK VV Q+G++I+DLLL++ QP+KICS+IG+C FDGT GVSM 
Sbjct: 306 ITMINHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMG 365

Query: 385 IESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQG 444
           IESV ++++ + S G  DA CSACEMAV W+  +L+QN TQE +++YVN+LC+R  S  G
Sbjct: 366 IESVVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMG 425

Query: 445 ETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLW 499
           E+ VDC ++S MPTVS TIG KVF+L  E+Y+LKVGEG  AQCISGFI LD+ PPRGPLW
Sbjct: 426 ESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLW 485

BLAST of Cp4.1LG10g09860 vs. ExPASy Swiss-Prot
Match: Q42456 (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 612.1 bits (1577), Expect = 1.1e-173
Identity = 300/483 (62.11%), Postives = 372/483 (77.02%), Query Frame = 0

Query: 31  LLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKK-VKFLGSKHDQWVNDVG 90
           LL  +L  +   +++ EGL+RI LKK  +++N  + A +  ++  + LG +    +   G
Sbjct: 10  LLAAVLLQALLPASAAEGLVRIALKKRPIDENSRVAARLSGEEGARRLGLRGANSLG--G 69

Query: 91  ESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIACFFHAR 150
                DIVALKNYM+AQY+GEIG+GTPPQKFTVIFDTGSSNLWVPS+KC FSIACFFH+R
Sbjct: 70  GGGEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACFFHSR 129

Query: 151 YQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSMTFIAA 210
           Y+SG+SSTY++NG  AAIQYGTG+I+GFFS D+V VGD+VV+DQ+FIE T    +TF+ A
Sbjct: 130 YKSGQSSTYQKNGKPAAIQYGTGSIAGFFSEDSVTVGDLVVKDQEFIEATKEPGLTFMVA 189

Query: 211 KFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFGGVDPK 270
           KFDGILGLGFQEIS GDAVPVWY MV Q LV EPVFSFW NR++ E EGGEIVFGG+DP 
Sbjct: 190 KFDGILGLGFQEISVGDAVPVWYKMVEQGLVSEPVFSFWFNRHSDEGEGGEIVFGGMDPS 249

Query: 271 HFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL------------ 330
           H+KG HTYVPV+ KGYWQF +GD+LIGG+ T +CA GCSAIADSGTSL            
Sbjct: 250 HYKGNHTYVPVSQKGYWQFEMGDVLIGGKTTGFCASGCSAIADSGTSLLAGPTAIITEIN 309

Query: 331 -AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMKIESVA 390
             IGA G+   ECK VVSQ+G+ I+DLLLA+ QP KICS++G+C FDG HGVS  I+SV 
Sbjct: 310 EKIGATGVVSQECKTVVSQYGQQILDLLLAETQPSKICSQVGLCTFDGKHGVSAGIKSVV 369

Query: 391 NEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQGETLVD 450
           +++ G S+G  S  MC+ACEMAV WM ++L QNKTQ+ +++Y+N+LCD+  S  GE+ VD
Sbjct: 370 DDEAGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVD 429

Query: 451 CGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLWILGDV 499
           CG ++ MP +SFTIG K F L  E+YILKVGEG+AAQCISGF  +DIPPPRGPLWILGDV
Sbjct: 430 CGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWILGDV 489

BLAST of Cp4.1LG10g09860 vs. ExPASy Swiss-Prot
Match: P42210 (Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 3.8e-171
Identity = 297/487 (60.99%), Postives = 372/487 (76.39%), Query Frame = 0

Query: 27  LLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGSKHDQWVN 86
           LL ++LLL      AS A  EGL+RI LKK  +++N  +   +   + + L S      N
Sbjct: 10  LLAAVLLLQTVLPAASEA--EGLVRIALKKRPIDRNSRVATGLSGGEEQPLLS----GAN 69

Query: 87  DVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIACFF 146
            +   +  DIVALKNYM+AQY+GEIG+GTPPQKFTVIFDTGSSNLWVPS+KC FSIAC+ 
Sbjct: 70  PLRSEEEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACYL 129

Query: 147 HARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSMTF 206
           H+RY++G SSTY++NG  AAIQYGTG+I+G+FS D+V VGD+VV+DQ+FIE T    +TF
Sbjct: 130 HSRYKAGASSTYKKNGKPAAIQYGTGSIAGYFSEDSVTVGDLVVKDQEFIEATKEPGITF 189

Query: 207 IAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFGGV 266
           + AKFDGILGLGF+EIS G AVPVWY M+ Q LV +PVFSFWLNR+  E EGGEI+FGG+
Sbjct: 190 LVAKFDGILGLGFKEISVGKAVPVWYKMIEQGLVSDPVFSFWLNRHVDEGEGGEIIFGGM 249

Query: 267 DPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL--------- 326
           DPKH+ G+HTYVPVT KGYWQF++GD+L+GG+ T +CA GC+AIADSGTSL         
Sbjct: 250 DPKHYVGEHTYVPVTQKGYWQFDMGDVLVGGKSTGFCAGGCAAIADSGTSLLAGPTAIIT 309

Query: 327 ----AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMKIE 386
                IGAAG+   ECK +VSQ+G+ I+DLLLA+ QP+KICS++G+C FDGT GVS  I 
Sbjct: 310 EINEKIGAAGVVSQECKTIVSQYGQQILDLLLAETQPKKICSQVGLCTFDGTRGVSAGIR 369

Query: 387 SVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQGET 446
           SV +++  +S+G  +D MCSACEMAV WM ++L QNKTQ+ ++DYVN+LC+R  S  GE+
Sbjct: 370 SVVDDEPVKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLPSPMGES 429

Query: 447 LVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLWIL 500
            VDCG +  MP + FTIG K F L  E+YILKVGEG+AAQCISGF  +DIPPPRGPLWIL
Sbjct: 430 AVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPLWIL 489

BLAST of Cp4.1LG10g09860 vs. NCBI nr
Match: XP_023544281.1 (aspartic proteinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953 bits (2464), Expect = 0.0
Identity = 479/492 (97.36%), Postives = 479/492 (97.36%), Query Frame = 0

Query: 20  MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 79
           MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS
Sbjct: 1   MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 60

Query: 80  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 139
           KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV
Sbjct: 61  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 120

Query: 140 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETT 199
           FSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETT
Sbjct: 121 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETT 180

Query: 200 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGG 259
           SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGG
Sbjct: 181 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGG 240

Query: 260 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL-- 319
           EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL  
Sbjct: 241 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSLLA 300

Query: 320 -----------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTH 379
                      AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTH
Sbjct: 301 GPSTIVTLINRAIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTH 360

Query: 380 GVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 439
           GVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD
Sbjct: 361 GVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 420

Query: 440 SNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 498
           SNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR
Sbjct: 421 SNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 480

BLAST of Cp4.1LG10g09860 vs. NCBI nr
Match: KAG7033959.1 (Aspartic proteinase A1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 469/495 (94.75%), Postives = 477/495 (96.36%), Query Frame = 0

Query: 17  RVTMRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKF 76
           +VTMRNSLKPLLVSLL+LILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKF
Sbjct: 25  QVTMRNSLKPLLVSLLVLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKF 84

Query: 77  LGSKHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSS 136
           LGSKHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSS
Sbjct: 85  LGSKHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSS 144

Query: 137 KCVFSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFI 196
           KCVFSIACFFHARYQSGRSSTYRRNGTSAAIQYG+GAISGFFSYDNVQVGDV+VRDQQFI
Sbjct: 145 KCVFSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRDQQFI 204

Query: 197 ETTSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEE 256
           ETTSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNA+EE
Sbjct: 205 ETTSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAEEE 264

Query: 257 EGGEIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTS 316
           EGGE+VFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGG+PTEYCA GCSAIADSGTS
Sbjct: 265 EGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCASGCSAIADSGTS 324

Query: 317 L-------------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFD 376
           L             AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVC FD
Sbjct: 325 LLAGPSTIVTLINRAIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCTFD 384

Query: 377 GTHGVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLC 436
           GTHGVSMKIES+ NEK GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLC
Sbjct: 385 GTHGVSMKIESMVNEK-GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLC 444

Query: 437 DRDSNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIP 496
           DRD NQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIP
Sbjct: 445 DRDLNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIP 504

Query: 497 PPRGPLWILGDVFMG 498
           PPRGPLWILGDVFMG
Sbjct: 505 PPRGPLWILGDVFMG 518

BLAST of Cp4.1LG10g09860 vs. NCBI nr
Match: KAG6603789.1 (Aspartic proteinase A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 930 bits (2404), Expect = 0.0
Identity = 468/494 (94.74%), Postives = 474/494 (95.95%), Query Frame = 0

Query: 18  VTMRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFL 77
           VTM NSLKPLLVSLL+LILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFL
Sbjct: 23  VTMGNSLKPLLVSLLVLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFL 82

Query: 78  GSKHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSK 137
           GSKHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFT IFDTGSSNLWVPSSK
Sbjct: 83  GSKHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTAIFDTGSSNLWVPSSK 142

Query: 138 CVFSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIE 197
           CVFSIACFFHARYQSGRSSTYRRNGTSAAIQYG+GAISGFFSYDNVQVGDVVVRDQQFIE
Sbjct: 143 CVFSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVVVRDQQFIE 202

Query: 198 TTSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEE 257
           TTSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNA+EEE
Sbjct: 203 TTSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAEEEE 262

Query: 258 GGEIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL 317
           GGE+VFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGG+PTEYCA GCSAIADSGTSL
Sbjct: 263 GGELVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCASGCSAIADSGTSL 322

Query: 318 -------------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDG 377
                        AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVC FDG
Sbjct: 323 LAGPSTIVTLINRAIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCTFDG 382

Query: 378 THGVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCD 437
           THGVSMKIES+ NEK GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCD
Sbjct: 383 THGVSMKIESMVNEK-GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCD 442

Query: 438 RDSNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPP 497
           RD NQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPP
Sbjct: 443 RDLNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPP 502

BLAST of Cp4.1LG10g09860 vs. NCBI nr
Match: XP_022950077.1 (aspartic proteinase-like [Cucurbita moschata])

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 467/492 (94.92%), Postives = 473/492 (96.14%), Query Frame = 0

Query: 20  MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 79
           M NSLKPLLVSLL+LILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS
Sbjct: 1   MGNSLKPLLVSLLVLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 60

Query: 80  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 139
           KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV
Sbjct: 61  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 120

Query: 140 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETT 199
           FSIACFFHARYQSGRSSTYRRNGTSAAIQYG+GAISGFFSYDNVQVGDVVVRDQQFIETT
Sbjct: 121 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVVVRDQQFIETT 180

Query: 200 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGG 259
           SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNA+EEEGG
Sbjct: 181 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAEEEEGG 240

Query: 260 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL-- 319
           E+VFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGG+PTEYCA GCSAIADSGTSL  
Sbjct: 241 ELVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCASGCSAIADSGTSLLA 300

Query: 320 -----------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTH 379
                      AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVC FDGTH
Sbjct: 301 GPSTIVTLINRAIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCTFDGTH 360

Query: 380 GVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 439
           GVSMKIES+ NEK GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD
Sbjct: 361 GVSMKIESMVNEK-GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 420

Query: 440 SNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 498
            NQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR
Sbjct: 421 LNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 480

BLAST of Cp4.1LG10g09860 vs. NCBI nr
Match: XP_022977721.1 (aspartic proteinase-like [Cucurbita maxima])

HSP 1 Score: 926 bits (2393), Expect = 0.0
Identity = 464/492 (94.31%), Postives = 472/492 (95.93%), Query Frame = 0

Query: 20  MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 79
           MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKV+FLGS
Sbjct: 1   MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVEFLGS 60

Query: 80  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 139
           KHDQW ND+GESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV
Sbjct: 61  KHDQWGNDLGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 120

Query: 140 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETT 199
           FSIACFFHARYQSGRSSTYRRNGTSAAIQYG+GAISGFFSYDNVQVGDVVVR+QQFIETT
Sbjct: 121 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVVVRNQQFIETT 180

Query: 200 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGG 259
           SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMV QKLVKEPVFSFWLNRNA+EEEGG
Sbjct: 181 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVKQKLVKEPVFSFWLNRNAEEEEGG 240

Query: 260 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL-- 319
           EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGG+PTEYCARGCSAIADSGTSL  
Sbjct: 241 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCARGCSAIADSGTSLLA 300

Query: 320 -----------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTH 379
                      AIGAAGIGRPECK VVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDG+H
Sbjct: 301 GPSTIVTLINRAIGAAGIGRPECKEVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGSH 360

Query: 380 GVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 439
           GVS KIESV NEKDG SSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD
Sbjct: 361 GVSKKIESVVNEKDGTSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 420

Query: 440 SNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 498
            N+GETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR
Sbjct: 421 LNEGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 480

BLAST of Cp4.1LG10g09860 vs. ExPASy TrEMBL
Match: A0A6J1GDT6 (aspartic proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453266 PE=3 SV=1)

HSP 1 Score: 928 bits (2399), Expect = 0.0
Identity = 467/492 (94.92%), Postives = 473/492 (96.14%), Query Frame = 0

Query: 20  MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 79
           M NSLKPLLVSLL+LILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS
Sbjct: 1   MGNSLKPLLVSLLVLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 60

Query: 80  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 139
           KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV
Sbjct: 61  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 120

Query: 140 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETT 199
           FSIACFFHARYQSGRSSTYRRNGTSAAIQYG+GAISGFFSYDNVQVGDVVVRDQQFIETT
Sbjct: 121 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVVVRDQQFIETT 180

Query: 200 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGG 259
           SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNA+EEEGG
Sbjct: 181 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAEEEEGG 240

Query: 260 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL-- 319
           E+VFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGG+PTEYCA GCSAIADSGTSL  
Sbjct: 241 ELVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCASGCSAIADSGTSLLA 300

Query: 320 -----------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTH 379
                      AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVC FDGTH
Sbjct: 301 GPSTIVTLINRAIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCTFDGTH 360

Query: 380 GVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 439
           GVSMKIES+ NEK GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD
Sbjct: 361 GVSMKIESMVNEK-GRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 420

Query: 440 SNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 498
            NQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR
Sbjct: 421 LNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 480

BLAST of Cp4.1LG10g09860 vs. ExPASy TrEMBL
Match: A0A6J1IKS1 (aspartic proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111477945 PE=3 SV=1)

HSP 1 Score: 926 bits (2393), Expect = 0.0
Identity = 464/492 (94.31%), Postives = 472/492 (95.93%), Query Frame = 0

Query: 20  MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS 79
           MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKV+FLGS
Sbjct: 1   MRNSLKPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVEFLGS 60

Query: 80  KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 139
           KHDQW ND+GESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV
Sbjct: 61  KHDQWGNDLGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCV 120

Query: 140 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETT 199
           FSIACFFHARYQSGRSSTYRRNGTSAAIQYG+GAISGFFSYDNVQVGDVVVR+QQFIETT
Sbjct: 121 FSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVVVRNQQFIETT 180

Query: 200 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGG 259
           SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMV QKLVKEPVFSFWLNRNA+EEEGG
Sbjct: 181 SMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVKQKLVKEPVFSFWLNRNAEEEEGG 240

Query: 260 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL-- 319
           EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGG+PTEYCARGCSAIADSGTSL  
Sbjct: 241 EIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCARGCSAIADSGTSLLA 300

Query: 320 -----------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTH 379
                      AIGAAGIGRPECK VVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDG+H
Sbjct: 301 GPSTIVTLINRAIGAAGIGRPECKEVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGSH 360

Query: 380 GVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 439
           GVS KIESV NEKDG SSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD
Sbjct: 361 GVSKKIESVVNEKDGTSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRD 420

Query: 440 SNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 498
            N+GETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR
Sbjct: 421 LNEGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPR 480

BLAST of Cp4.1LG10g09860 vs. ExPASy TrEMBL
Match: A0A6J1GDW5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111453267 PE=3 SV=1)

HSP 1 Score: 818 bits (2112), Expect = 1.71e-289
Identity = 410/429 (95.57%), Postives = 418/429 (97.44%), Query Frame = 0

Query: 500 MKGSLFFSSHPSMRTSVFSMDWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTHSDF 559
           MKGSLFFSSHPSMRTSV SM+WTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFT SD 
Sbjct: 1   MKGSLFFSSHPSMRTSVVSMEWTRGPTIGRGSSAAVSVATDLCSGQVLAVKSVEFTRSDC 60

Query: 560 LKREQRILSRFNCPRVIAYKGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEAT 619
           LKREQRILSRFNCPRVIAYKGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEA 
Sbjct: 61  LKREQRILSRFNCPRVIAYKGFDVTLENGELMCNLLMEFAPGGSILDVMEAAGGRLDEAR 120

Query: 620 AQFYTREILSGLEYVHSNGVVHCDIKCCNILVGEDGIKIADFGCARQVEEDSSRNLVGTP 679
           AQFYTREILSGLEYVH+NGVVHCDIKCCNILVGEDGIKIADFGCARQVE+ S RNL GT 
Sbjct: 121 AQFYTREILSGLEYVHTNGVVHCDIKCCNILVGEDGIKIADFGCARQVEDVSCRNLAGTL 180

Query: 680 MFMAPEVARGEKQGFAADVWAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPR 739
           +FMAPEVARGEKQGFAADVWAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPR
Sbjct: 181 IFMAPEVARGEKQGFAADVWAVGCAVIQMVTGRAPWVNVSDPVAAIYKIGSGDDLPEVPR 240

Query: 740 FMSEQGKDFLRRCLIRDPGERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMN 799
           F+S+QGKDFLRRCLIRDP ERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMN
Sbjct: 241 FISDQGKDFLRRCLIRDPEERWSVSELLKHPFVHEQKSPPKQNSRTPTSILEQESWYTMN 300

Query: 800 NSETVQSPIMPKLYRTPLQRIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQN 859
           NSETVQ PIMPKLYRTPLQRIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQ+
Sbjct: 301 NSETVQIPIMPKLYRTPLQRIQQLSEISKIQSSRIPNWDCDKDWVTVKSITMEEEDTEQS 360

Query: 860 EFVSVLELPCFSQSIKMATKNGVGDQNYIDVSISRESSKAKKRDLPAMSSVSNQIVSLNT 919
           EFVSVLELPCFSQSIKMATKNGVGDQ YI+VSISRESSKAKKRDLPAMSSVSNQIVSLNT
Sbjct: 361 EFVSVLELPCFSQSIKMATKNGVGDQTYINVSISRESSKAKKRDLPAMSSVSNQIVSLNT 420

Query: 920 NSFSNNHKL 928
           NSFSNNHKL
Sbjct: 421 NSFSNNHKL 429

BLAST of Cp4.1LG10g09860 vs. ExPASy TrEMBL
Match: A0A1S3B040 (aspartic proteinase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484625 PE=3 SV=1)

HSP 1 Score: 792 bits (2046), Expect = 6.35e-279
Identity = 397/494 (80.36%), Postives = 438/494 (88.66%), Query Frame = 0

Query: 23  SLKPLLVSLLLLIL-YSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLG--- 82
           S   LLVSLLLLIL YSS A+SASNEG +RIGLKKI+ ++N   KAL+ESKK +FLG   
Sbjct: 5   SFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFLGPSV 64

Query: 83  SKHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 142
            KH+QW N++GESKN+D V LKNY+DAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC
Sbjct: 65  GKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 124

Query: 143 VFSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIET 202
           VFS+ACFFHARYQSGRSSTY++NGTSAAIQYG+GAI+GFFS DNV+VGDVVVR+Q  IE 
Sbjct: 125 VFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQDLIEA 184

Query: 203 TSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEG 262
           TSMSSMTF+AAKFDGILGLGFQEISTG AVPVWYNMV QKLVKE VFSFWLNRNAKEEEG
Sbjct: 185 TSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAKEEEG 244

Query: 263 GEIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL- 322
           GE+VFGGVDPKHFKGQHTYVPVT KGYWQF+IGDILIGGE T+YCA GCSAIADSGTSL 
Sbjct: 245 GELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSGTSLL 304

Query: 323 ------------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGT 382
                       AIGAA +  PECKA+VSQHG++IMDLLLAK QPEKICS IGVC FD T
Sbjct: 305 AGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCTFDQT 364

Query: 383 HGVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR 442
             VS+KIE+V ++KDGRSSGGFS+AMCSACEMAVSW+ DEL+QNKTQE +ID VN+LCDR
Sbjct: 365 RDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNELCDR 424

Query: 443 DSNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPP 499
            SNQ ETLVDCGRISQMP+VSFTIGD+VFEL+++DYILKVGEGSAAQCISGFIPLDIPPP
Sbjct: 425 GSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPP 484

BLAST of Cp4.1LG10g09860 vs. ExPASy TrEMBL
Match: A0A5D3CRY9 (Aspartic proteinase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006300 PE=3 SV=1)

HSP 1 Score: 791 bits (2044), Expect = 1.28e-278
Identity = 397/494 (80.36%), Postives = 439/494 (88.87%), Query Frame = 0

Query: 23  SLKPLLVSLLLLIL-YSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGS-- 82
           S   LLVSLLLLIL YSS A+SASNEG +RIGLKKI+ ++N   KAL+ESKK +FLGS  
Sbjct: 5   SFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFLGSSV 64

Query: 83  -KHDQWVNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 142
            K++QW N++GESKN+D V LKNY+DAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC
Sbjct: 65  GKYNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 124

Query: 143 VFSIACFFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIET 202
           VFS+ACFFHARYQSGRSSTY++NGTSAAIQYG+GAI+GFFS DNV+VGDVVVR+Q  IE 
Sbjct: 125 VFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQDLIEA 184

Query: 203 TSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEG 262
           TSMSSMTF+AAKFDGILGLGFQEISTG AVPVWYNMV QKLVKE VFSFWLNRNAKEEEG
Sbjct: 185 TSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAKEEEG 244

Query: 263 GEIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL- 322
           GE+VFGGVDPKHFKGQHTYVPVT KGYWQF+IGDILIGGE T+YCA GCSAIADSGTSL 
Sbjct: 245 GELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSGTSLL 304

Query: 323 ------------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGT 382
                       AIGAA +  PECKA+VSQHG++IMDLLLAK QPEKICS IGVC FD T
Sbjct: 305 AGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCTFDQT 364

Query: 383 HGVSMKIESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR 442
             VS+KIE+V ++KDGRSSGGFS+AMCSACEMAVSW+ DEL+QNKTQE +ID VN+LCDR
Sbjct: 365 RDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNELCDR 424

Query: 443 DSNQGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPP 499
            SNQ ETLVDCGRISQMP+VSFTIGD+VFEL+++DYILKVGEGSAAQCISGFIPLDIPPP
Sbjct: 425 GSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLDIPPP 484

BLAST of Cp4.1LG10g09860 vs. TAIR 10
Match: AT1G62290.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 634.0 bits (1634), Expect = 1.9e-181
Identity = 304/484 (62.81%), Postives = 383/484 (79.13%), Query Frame = 0

Query: 30  SLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGSKHDQWVNDV- 89
           S+ +  L   TA S  N+G  R+GLKK+K++ N  L     SK+ + L S    + N++ 
Sbjct: 11  SVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYNNNLG 70

Query: 90  GESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIACFFHA 149
           G+S ++DIV LKNY+DAQYYGEI IGTPPQKFTVIFDTGSSNLWVPS KC FS++C+FHA
Sbjct: 71  GDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFSLSCYFHA 130

Query: 150 RYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSMTFIA 209
           +Y+S RSSTY+++G  AAI YG+G+ISGFFSYD V VGD+VV+DQ+FIETTS   +TF+ 
Sbjct: 131 KYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSEPGLTFLV 190

Query: 210 AKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFGGVDP 269
           AKFDG+LGLGFQEI+ G+A PVWYNM+ Q L+K PVFSFWLNR+ K EEGGEIVFGGVDP
Sbjct: 191 AKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEIVFGGVDP 250

Query: 270 KHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL----------- 329
           KHF+G+HT+VPVT +GYWQF++G++LI GE T YC  GCSAIADSGTSL           
Sbjct: 251 KHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGPTAVVAMI 310

Query: 330 --AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMKIESV 389
             AIGA+G+   +CK VV Q+G++I+DLLLA+ QP+KICS+IG+CA+DGTHGVSM IESV
Sbjct: 311 NKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGVSMGIESV 370

Query: 390 ANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQGETLV 449
            ++++ RSS G  DA C ACEMAV W+  +L+QN TQE +++Y+N++C+R  S  GE+ V
Sbjct: 371 VDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAV 430

Query: 450 DCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLWILGD 499
           DC ++S+MPTVSFTIG KVF+L  E+Y+LK+GEG  AQCISGF  LDIPPPRGPLWILGD
Sbjct: 431 DCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGD 490

BLAST of Cp4.1LG10g09860 vs. TAIR 10
Match: AT1G62290.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 634.0 bits (1634), Expect = 1.9e-181
Identity = 304/484 (62.81%), Postives = 383/484 (79.13%), Query Frame = 0

Query: 30  SLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGSKHDQWVNDV- 89
           S+ +  L   TA S  N+G  R+GLKK+K++ N  L     SK+ + L S    + N++ 
Sbjct: 11  SVFVSFLLFFTAYSKRNDGTFRVGLKKLKLDPNNRLATRFGSKQEEALRSSLRSYNNNLG 70

Query: 90  GESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIACFFHA 149
           G+S ++DIV LKNY+DAQYYGEI IGTPPQKFTVIFDTGSSNLWVPS KC FS++C+FHA
Sbjct: 71  GDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVPSGKCFFSLSCYFHA 130

Query: 150 RYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSMTFIA 209
           +Y+S RSSTY+++G  AAI YG+G+ISGFFSYD V VGD+VV+DQ+FIETTS   +TF+ 
Sbjct: 131 KYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQEFIETTSEPGLTFLV 190

Query: 210 AKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFGGVDP 269
           AKFDG+LGLGFQEI+ G+A PVWYNM+ Q L+K PVFSFWLNR+ K EEGGEIVFGGVDP
Sbjct: 191 AKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPKSEEGGEIVFGGVDP 250

Query: 270 KHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL----------- 329
           KHF+G+HT+VPVT +GYWQF++G++LI GE T YC  GCSAIADSGTSL           
Sbjct: 251 KHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSGTSLLAGPTAVVAMI 310

Query: 330 --AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMKIESV 389
             AIGA+G+   +CK VV Q+G++I+DLLLA+ QP+KICS+IG+CA+DGTHGVSM IESV
Sbjct: 311 NKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCAYDGTHGVSMGIESV 370

Query: 390 ANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQGETLV 449
            ++++ RSS G  DA C ACEMAV W+  +L+QN TQE +++Y+N++C+R  S  GE+ V
Sbjct: 371 VDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINEICERMPSPNGESAV 430

Query: 450 DCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLWILGD 499
           DC ++S+MPTVSFTIG KVF+L  E+Y+LK+GEG  AQCISGF  LDIPPPRGPLWILGD
Sbjct: 431 DCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTALDIPPPRGPLWILGD 490

BLAST of Cp4.1LG10g09860 vs. TAIR 10
Match: AT1G11910.1 (aspartic proteinase A1 )

HSP 1 Score: 632.5 bits (1630), Expect = 5.4e-181
Identity = 308/488 (63.11%), Postives = 378/488 (77.46%), Query Frame = 0

Query: 25  KPLLVSLLLLILYSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVKFLGSKHDQW 84
           + + VSL++  L   +A +  N+G  R+GLKK+K++    L A VESK+ K L +     
Sbjct: 6   RTVAVSLIVSFLLCFSAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRAYR--- 65

Query: 85  VNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIAC 144
              +G+S ++D+V LKNY+DAQYYGEI IGTPPQKFTV+FDTGSSNLWVPSSKC FS+AC
Sbjct: 66  ---LGDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSLAC 125

Query: 145 FFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSM 204
             H +Y+S RSSTY +NG +AAI YGTGAI+GFFS D V VGD+VV+DQ+FIE T    +
Sbjct: 126 LLHPKYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEPGI 185

Query: 205 TFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFG 264
           TF+ AKFDGILGLGFQEIS G A PVWYNM+ Q L+KEPVFSFWLNRNA EEEGGE+VFG
Sbjct: 186 TFVVAKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELVFG 245

Query: 265 GVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL------- 324
           GVDP HFKG+HTYVPVT KGYWQF++GD+LIGG PT +C  GCSAIADSGTSL       
Sbjct: 246 GVDPNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPTTI 305

Query: 325 ------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMK 384
                 AIGAAG+   +CK VV Q+G++I+DLLL++ QP+KICS+IG+C FDGT GVSM 
Sbjct: 306 ITMINHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVSMG 365

Query: 385 IESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR-DSNQG 444
           IESV ++++ + S G  DA CSACEMAV W+  +L+QN TQE +++YVN+LC+R  S  G
Sbjct: 366 IESVVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSPMG 425

Query: 445 ETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLW 499
           E+ VDC ++S MPTVS TIG KVF+L  E+Y+LKVGEG  AQCISGFI LD+ PPRGPLW
Sbjct: 426 ESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGPLW 485

BLAST of Cp4.1LG10g09860 vs. TAIR 10
Match: AT4G04460.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 590.5 bits (1521), Expect = 2.4e-168
Identity = 293/489 (59.92%), Postives = 371/489 (75.87%), Query Frame = 0

Query: 27  LLVSLLLLILYSSTASSASN-EGLMRIGLKKIKVNK-NILLKALVESKKVKFLGSKHDQW 86
           LLV LL  ++  STAS   N +G +RIGLKK K+++ N L   L    +      KH   
Sbjct: 9   LLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKNRGSHWSPKHYFR 68

Query: 87  VNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIAC 146
           +ND    +N+D+V LKNY+DAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS+KC  S+AC
Sbjct: 69  LND----ENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYLSVAC 128

Query: 147 FFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSM 206
           +FH++Y++ +SS+YR+NG  A+I+YGTGAISG+FS D+V+VGD+VV++Q+FIE TS   +
Sbjct: 129 YFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATSEPGI 188

Query: 207 TFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFG 266
           TF+ AKFDGILGLGF+EIS G++ PVWYNMV + LVKEP+FSFWLNRN K+ EGGEIVFG
Sbjct: 189 TFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGEIVFG 248

Query: 267 GVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL------- 326
           GVDPKHFKG+HT+VPVT KGYWQF++GD+ I G+PT YCA+GCSAIADSGTSL       
Sbjct: 249 GVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTGPSTV 308

Query: 327 ------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMK 386
                 AIGA GI   ECKAVV Q+GK++++ LLA+  P+K+CS+IGVCA+DGT  VSM 
Sbjct: 309 ITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQEDPKKVCSQIGVCAYDGTQSVSMG 368

Query: 387 IESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRDSNQG- 446
           I+SV    D  +SG  + AMCSACEMA  WM  EL QN+TQE ++ Y  +LCD    Q  
Sbjct: 369 IQSVV---DDGTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQ 428

Query: 447 ETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLW 500
           ++ VDCGR+S MP V+F+IG + F+L  +DYI K+GEG  +QC SGF  +DI PPRGPLW
Sbjct: 429 QSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLW 488

BLAST of Cp4.1LG10g09860 vs. TAIR 10
Match: AT4G04460.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 582.4 bits (1500), Expect = 6.4e-166
Identity = 292/489 (59.71%), Postives = 369/489 (75.46%), Query Frame = 0

Query: 27  LLVSLLLLILYSSTASSASN-EGLMRIGLKKIKVNK-NILLKALVESKKVKFLGSKHDQW 86
           LLV LL  ++  STAS   N +G +RIGLKK K+++ N L   L    +      KH   
Sbjct: 9   LLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKNRGSHWSPKHYFR 68

Query: 87  VNDVGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCVFSIAC 146
           +ND    +N+D+V LKNY+DAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS+KC  S+AC
Sbjct: 69  LND----ENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYLSVAC 128

Query: 147 FFHARYQSGRSSTYRRNGTSAAIQYGTGAISGFFSYDNVQVGDVVVRDQQFIETTSMSSM 206
           +FH++Y++ +SS+YR+NG  A+I+YGTGAISG+FS D+V+VGD+VV++Q+FIE TS   +
Sbjct: 129 YFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATSEPGI 188

Query: 207 TFIAAKFDGILGLGFQEISTGDAVPVWYNMVNQKLVKEPVFSFWLNRNAKEEEGGEIVFG 266
           TF+ AKFDGILGLGF+EIS G++ PVWYNMV + LVKEP+FSFWLNRN K+ EGGEIVFG
Sbjct: 189 TFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGEIVFG 248

Query: 267 GVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGEPTEYCARGCSAIADSGTSL------- 326
           GVDPKHFKG+HT+VPVT KGYWQF++GD+ I G+PT YCA+GCSAIADSGTSL       
Sbjct: 249 GVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTGPSTV 308

Query: 327 ------AIGAAGIGRPECKAVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGTHGVSMK 386
                 AIGA GI   ECKAVV Q+GK++++ LLA    +K+CS+IGVCA+DGT  VSM 
Sbjct: 309 ITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLA----QKVCSQIGVCAYDGTQSVSMG 368

Query: 387 IESVANEKDGRSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDRDSNQG- 446
           I+SV    D  +SG  + AMCSACEMA  WM  EL QN+TQE ++ Y  +LCD    Q  
Sbjct: 369 IQSVV---DDGTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIPTQNQ 428

Query: 447 ETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPPRGPLW 500
           ++ VDCGR+S MP V+F+IG + F+L  +DYI K+GEG  +QC SGF  +DI PPRGPLW
Sbjct: 429 QSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPRGPLW 486