Cp4.1LG10g09850 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG10g09850 vs. ExPASy Swiss-Prot
Match: O28972 (Cell division cycle protein 48 homolog AF_1297 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1297 PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 3.4e-25
Identity = 78/217 (35.94%), Postives = 118/217 (54.38%), Query Frame = 0

Query: 593 EISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP---- 652
           ++++++I G  ++   + + I L L +PELF  +            P+ VL  GPP    
Sbjct: 178 DVTYEDIGGLKRELRLVREMIELPLKHPELFQRLG--------IEPPKGVLLYGPPGTGK 237

Query: 653 --VISILQNGVPLVYVPLE--VIMSKYYGESERLLGQVFSLANELSTGAIIFLDEVDSFA 712
             +   + N V   ++P+    IMSKYYGESE+ L ++F  A E +  +IIF+DE+DS A
Sbjct: 238 TLIAKAVANEVDAHFIPISGPEIMSKYYGESEQRLREIFEEAKE-NAPSIIFIDEIDSIA 297

Query: 713 IARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPAL--ISRFDLMITFG 772
             R+    E  RRV++ LL  +DG E    V+VIAATNR   +DPAL    RFD  I  G
Sbjct: 298 PKREEVTGEVERRVVAQLLALMDGLEARGDVIVIAATNRPDAIDPALRRPGRFDREIEIG 357

Query: 773 LPDERNRQEIAAQYAKQLTKPE---LNEFAKNTEGLI 797
           +PD+  R+EI   + +++   E   L E A+ T G +
Sbjct: 358 VPDKEGRKEILEIHTRKMPLAEDVDLEELAELTNGFV 385

BLAST of Cp4.1LG10g09850 vs. ExPASy Swiss-Prot
Match: O05209 (VCP-like ATPase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=vat PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 3.2e-23
Identity = 74/211 (35.07%), Postives = 114/211 (54.03%), Query Frame = 0

Query: 592 NEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPPVIS 651
           + IS+++I G  +Q  +I + I L L +PELF+ +            P+ V+  GPP   
Sbjct: 185 SRISYEDIGGLSEQLGKIREMIELPLKHPELFERLG--------ITPPKGVILYGPPGTG 244

Query: 652 --------ILQNGVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIFLDEVDSF 711
                     ++G   + +    IMSKYYG+SE+ L ++FS A E +  +IIF+DE+DS 
Sbjct: 245 KTLIARAVANESGANFLSINGPEIMSKYYGQSEQKLREIFSKAEE-TAPSIIFIDEIDSI 304

Query: 712 AIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPAL--ISRFDLMITF 771
           A  R+    E  RRV++ LL  +DG ++   V+VI ATNR   +DPAL    RFD  I  
Sbjct: 305 APKREEVQGEVERRVVAQLLTLMDGMKERGHVIVIGATNRIDAIDPALRRPGRFDREIEI 364

Query: 772 GLPDERNRQEIAAQYAKQ----LTKPELNEF 789
           G+PD   R+EI   + +     +++ E N+F
Sbjct: 365 GVPDRNGRKEILMIHTRNMPLGMSEEEKNKF 386

BLAST of Cp4.1LG10g09850 vs. ExPASy Swiss-Prot
Match: Q5AWS6 (Cell division control protein 48 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=cdc48 PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 1.6e-22
Identity = 82/238 (34.45%), Postives = 116/238 (48.74%), Query Frame = 0

Query: 572 LESMGVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTR 631
           + S G  +   DE    ++ NE+ +D+I G  +Q ++I + + L L +P+LF  I     
Sbjct: 203 IHSEGEPIQREDE---ENNLNEVGYDDIGGCRKQMAQIRELVELPLRHPQLFKSIG---- 262

Query: 632 RKFESNRPRAVLFEGPPVIS--------ILQNGVPLVYVPLEVIMSKYYGESERLLGQVF 691
                  PR +L  GPP             + G     +    IMSK  GESE  L + F
Sbjct: 263 ----IKPPRGILMYGPPGTGKTLMARAVANETGAFFFLINGPEIMSKMAGESESNLRKAF 322

Query: 692 SLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNR 751
             A E ++ AIIF+DE+DS A  R+    E  RRV+S LL  +DG +    VVV+AATNR
Sbjct: 323 EEA-EKNSPAIIFIDEIDSIAPKREKTNGEVERRVVSQLLTLMDGMKARSNVVVMAATNR 382

Query: 752 KQDLDPAL--ISRFDLMITFGLPDERNRQEIAAQYAKQLTKPE---LNEFAKNTEGLI 797
              +DPAL    RFD  +  G+PD   R EI + + K +   E   L   A  T G +
Sbjct: 383 PNSIDPALRRFGRFDREVDIGIPDPTGRLEILSIHTKNMKLGEDVDLETIAAETHGYV 428

BLAST of Cp4.1LG10g09850 vs. ExPASy Swiss-Prot
Match: P25694 (Cell division control protein 48 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CDC48 PE=1 SV=3)

HSP 1 Score: 109.8 bits (273), Expect = 1.6e-22
Identity = 78/221 (35.29%), Postives = 108/221 (48.87%), Query Frame = 0

Query: 589 SSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP 648
           ++ NE+ +D+I G  +Q ++I + + L L +P+LF  I            PR VL  GPP
Sbjct: 206 NNMNEVGYDDIGGCRKQMAQIREMVELPLRHPQLFKAIG--------IKPPRGVLMYGPP 265

Query: 649 VIS--------ILQNGVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIFLDEV 708
                        + G     +    +MSK  GESE  L + F  A E +  AIIF+DE+
Sbjct: 266 GTGKTLMARAVANETGAFFFLINGPEVMSKMAGESESNLRKAFEEA-EKNAPAIIFIDEI 325

Query: 709 DSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPAL--ISRFDLM 768
           DS A  RD    E  RRV+S LL  +DG +    VVVIAATNR   +DPAL    RFD  
Sbjct: 326 DSIAPKRDKTNGEVERRVVSQLLTLMDGMKARSNVVVIAATNRPNSIDPALRRFGRFDRE 385

Query: 769 ITFGLPDERNRQEIAAQYAKQL---TKPELNEFAKNTEGLI 797
           +  G+PD   R E+   + K +      +L   A  T G +
Sbjct: 386 VDIGIPDATGRLEVLRIHTKNMKLADDVDLEALAAETHGYV 417

BLAST of Cp4.1LG10g09850 vs. ExPASy Swiss-Prot
Match: Q8TX03 (Proteasome-activating nucleotidase OS=Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) OX=190192 GN=pan PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.0e-22
Identity = 78/221 (35.29%), Postives = 115/221 (52.04%), Query Frame = 0

Query: 593 EISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP---- 652
           ++S+D+I G D+Q  EI + +   L  PELF+++            P+ VL  GPP    
Sbjct: 175 DVSYDDIGGLDEQIREIREVVEKPLKEPELFEKVG--------VEPPKGVLLYGPPGTGK 234

Query: 653 --VISILQNGVPLVYVPLEV--IMSKYYGESERLLGQVFSLANELSTGAIIFLDEVDSFA 712
             +   + N     ++ L    ++ K+ GE  RL+ ++F LA E    +IIF+DE+D+  
Sbjct: 235 TLLAKAVANHADATFIRLAAPELVQKFIGEGARLVRELFELARE-KAPSIIFIDEIDAIG 294

Query: 713 IARDSEMHEAT------RRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALI--SRFD 772
             R   M +AT      +R L+ LL ++DGF+    + VIAATNRK  LDPAL+   RFD
Sbjct: 295 ARR---MRDATSGDREVQRTLTQLLAEMDGFDPLDDIKVIAATNRKDILDPALLRPGRFD 354

Query: 773 LMITFGLPDERNRQEIAAQYAKQLTKPE---LNEFAKNTEG 795
             I   LPDE  R EI   + + +   E   L + AK TEG
Sbjct: 355 RHIKIPLPDEEGRYEIFKIHTRDMNLAEDVDLQKLAKITEG 383

BLAST of Cp4.1LG10g09850 vs. NCBI nr
Match: XP_023544078.1 (katanin p60 ATPase-containing subunit A1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1033 bits (2671), Expect = 0.0
Identity = 549/600 (91.50%), Postives = 551/600 (91.83%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRHHPEHLKPGFLSNGREPCFLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHHPEHLKPGFLSNGREPCFLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 399
           AIVASMFGVGAMEIAYAEAEESAGTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAGTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 120

Query: 400 KYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 459
           KYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS
Sbjct: 121 KYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 180

Query: 460 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFNKLSATN 519
           PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT            
Sbjct: 181 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT------------ 240

Query: 520 YEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISSLESM 579
            EIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISSLESM
Sbjct: 241 -EIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISSLESM 300

Query: 580 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 639
           GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE
Sbjct: 301 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 360

Query: 640 SNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 699
           SNRPRAVLFEGPP         +   Q GVPLVYVPLEVIMSKYYGESERLLGQVFSLAN
Sbjct: 361 SNRPRAVLFEGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 420

Query: 700 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 759
           ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL
Sbjct: 421 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 480

Query: 760 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL------------ 819
           DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEG+            
Sbjct: 481 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQA 540

Query: 820 -------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 848
                  ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA
Sbjct: 541 ERSWASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 587

BLAST of Cp4.1LG10g09850 vs. NCBI nr
Match: XP_022977688.1 (spermatogenesis-associated protein 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 541/600 (90.17%), Postives = 546/600 (91.00%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPC LYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCLLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 120

Query: 400 KYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 459
           KYGACPRFTVGVKGQKVSIKFQVPS+CEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS
Sbjct: 121 KYGACPRFTVGVKGQKVSIKFQVPSNCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 180

Query: 460 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFNKLSATN 519
           PVAWQLSLTRPKTQREAGGNEGNSLEIDAGD DLSVLIFHSLVSSDKT            
Sbjct: 181 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDGDLSVLIFHSLVSSDKT------------ 240

Query: 520 YEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISSLESM 579
            EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSISSLESM
Sbjct: 241 -EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESM 300

Query: 580 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 639
           GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE
Sbjct: 301 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 360

Query: 640 SNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 699
           SNRPRAVLFEGPP         +   Q GVPLVYVPLEVIMSKYYGESERLLGQVFSLAN
Sbjct: 361 SNRPRAVLFEGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 420

Query: 700 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 759
           ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL
Sbjct: 421 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 480

Query: 760 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL------------ 819
           DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEG+            
Sbjct: 481 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQA 540

Query: 820 -------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 848
                  ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA
Sbjct: 541 ERSWASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 587

BLAST of Cp4.1LG10g09850 vs. NCBI nr
Match: KAG6603800.1 (hypothetical protein SDJN03_04409, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 541/604 (89.57%), Postives = 545/604 (90.23%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPCFLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPP----KDLSTHADLEEIAKKERQRINEQLIR 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPP    KDLSTHADLEEIAKKERQRINEQLIR
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPKDLSTHADLEEIAKKERQRINEQLIR 120

Query: 400 NKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR 459
           NKGMKYGACPRFTVGVKGQ VSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR
Sbjct: 121 NKGMKYGACPRFTVGVKGQTVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR 180

Query: 460 AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFNKL 519
           AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT        
Sbjct: 181 AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT-------- 240

Query: 520 SATNYEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISS 579
                EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSISS
Sbjct: 241 -----EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISS 300

Query: 580 LESMGVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTR 639
           LESMGVKVYGLDE HVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTR
Sbjct: 301 LESMGVKVYGLDEQHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTR 360

Query: 640 RKFESNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQVF 699
           RKFESNRPRAVLFEGPP         +   Q GVPLVYVPLEVIMSKYYGESERLLGQVF
Sbjct: 361 RKFESNRPRAVLFEGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVF 420

Query: 700 SLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNR 759
           SLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNR
Sbjct: 421 SLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNR 480

Query: 760 KQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL-------- 819
           KQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEG+        
Sbjct: 481 KQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDI 540

Query: 820 -----------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKK 848
                      ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHK KDSNIRTKK
Sbjct: 541 CQQAERSWASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKFKDSNIRTKK 591

BLAST of Cp4.1LG10g09850 vs. NCBI nr
Match: KAG7033971.1 (pan, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1008 bits (2606), Expect = 0.0
Identity = 541/604 (89.57%), Postives = 545/604 (90.23%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPCFLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPP----KDLSTHADLEEIAKKERQRINEQLIR 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPP    KDLSTHADLEEIAKKERQRINEQLIR
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPKDLSTHADLEEIAKKERQRINEQLIR 120

Query: 400 NKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR 459
           NKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR
Sbjct: 121 NKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR 180

Query: 460 AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFNKL 519
           AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT        
Sbjct: 181 AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT-------- 240

Query: 520 SATNYEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISS 579
                EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSISS
Sbjct: 241 -----EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISS 300

Query: 580 LESMGVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTR 639
           LESMGVKVYGLDE HVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTR
Sbjct: 301 LESMGVKVYGLDEQHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTR 360

Query: 640 RKFESNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQVF 699
           RKFESNRPRAVLFEGPP         +   Q GVPLVYVPLEVIMSKYYGESERLLGQVF
Sbjct: 361 RKFESNRPRAVLFEGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVF 420

Query: 700 SLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNR 759
           SLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNR
Sbjct: 421 SLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNR 480

Query: 760 KQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL-------- 819
           KQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEG+        
Sbjct: 481 KQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDI 540

Query: 820 -----------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKK 848
                      ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNI  KK
Sbjct: 541 CQQAERSWASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIGMKK 591

BLAST of Cp4.1LG10g09850 vs. NCBI nr
Match: XP_022949948.1 (nuclear valosin-containing protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1006 bits (2601), Expect = 0.0
Identity = 540/606 (89.11%), Postives = 545/606 (89.93%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPCFLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPP------KDLSTHADLEEIAKKERQRINEQL 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPP      KDLSTHADLEEIAKKERQRINEQL
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPPPKDLSTHADLEEIAKKERQRINEQL 120

Query: 400 IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML 459
           IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML
Sbjct: 121 IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML 180

Query: 460 LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFN 519
           LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT      
Sbjct: 181 LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT------ 240

Query: 520 KLSATNYEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSI 579
                  EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSI
Sbjct: 241 -------EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSI 300

Query: 580 SSLESMGVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG 639
           SSLESMGVKVYGLDE HVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG
Sbjct: 301 SSLESMGVKVYGLDEQHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG 360

Query: 640 TRRKFESNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQ 699
           TRRKFESNRPRAVLFEGPP         +   Q GVPL+YVPLEVIMSKYYGESERLLGQ
Sbjct: 361 TRRKFESNRPRAVLFEGPPGTGKTSSARVIANQAGVPLLYVPLEVIMSKYYGESERLLGQ 420

Query: 700 VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT 759
           VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT
Sbjct: 421 VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT 480

Query: 760 NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL------ 819
           NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEG+      
Sbjct: 481 NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIR 540

Query: 820 -------------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRT 848
                        ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNI  
Sbjct: 541 DICQQAERSWASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIGM 593

BLAST of Cp4.1LG10g09850 vs. ExPASy TrEMBL
Match: A0A6J1IQS3 (spermatogenesis-associated protein 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477922 PE=3 SV=1)

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 541/600 (90.17%), Postives = 546/600 (91.00%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPC LYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCLLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 120

Query: 400 KYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 459
           KYGACPRFTVGVKGQKVSIKFQVPS+CEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS
Sbjct: 121 KYGACPRFTVGVKGQKVSIKFQVPSNCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 180

Query: 460 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFNKLSATN 519
           PVAWQLSLTRPKTQREAGGNEGNSLEIDAGD DLSVLIFHSLVSSDKT            
Sbjct: 181 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDGDLSVLIFHSLVSSDKT------------ 240

Query: 520 YEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISSLESM 579
            EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSISSLESM
Sbjct: 241 -EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESM 300

Query: 580 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 639
           GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE
Sbjct: 301 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 360

Query: 640 SNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 699
           SNRPRAVLFEGPP         +   Q GVPLVYVPLEVIMSKYYGESERLLGQVFSLAN
Sbjct: 361 SNRPRAVLFEGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 420

Query: 700 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 759
           ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL
Sbjct: 421 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 480

Query: 760 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL------------ 819
           DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEG+            
Sbjct: 481 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQA 540

Query: 820 -------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 848
                  ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA
Sbjct: 541 ERSWASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 587

BLAST of Cp4.1LG10g09850 vs. ExPASy TrEMBL
Match: A0A6J1GDJ0 (nuclear valosin-containing protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453188 PE=3 SV=1)

HSP 1 Score: 1006 bits (2601), Expect = 0.0
Identity = 540/606 (89.11%), Postives = 545/606 (89.93%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPCFLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPP------KDLSTHADLEEIAKKERQRINEQL 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPP      KDLSTHADLEEIAKKERQRINEQL
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPPPKDLSTHADLEEIAKKERQRINEQL 120

Query: 400 IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML 459
           IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML
Sbjct: 121 IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML 180

Query: 460 LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFN 519
           LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT      
Sbjct: 181 LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT------ 240

Query: 520 KLSATNYEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSI 579
                  EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSI
Sbjct: 241 -------EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSI 300

Query: 580 SSLESMGVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG 639
           SSLESMGVKVYGLDE HVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG
Sbjct: 301 SSLESMGVKVYGLDEQHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG 360

Query: 640 TRRKFESNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQ 699
           TRRKFESNRPRAVLFEGPP         +   Q GVPL+YVPLEVIMSKYYGESERLLGQ
Sbjct: 361 TRRKFESNRPRAVLFEGPPGTGKTSSARVIANQAGVPLLYVPLEVIMSKYYGESERLLGQ 420

Query: 700 VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT 759
           VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT
Sbjct: 421 VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT 480

Query: 760 NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL------ 819
           NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEG+      
Sbjct: 481 NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIR 540

Query: 820 -------------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRT 848
                        ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNI  
Sbjct: 541 DICQQAERSWASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIGM 593

BLAST of Cp4.1LG10g09850 vs. ExPASy TrEMBL
Match: A0A6J1IS35 (spermatogenesis-associated protein 5 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477922 PE=3 SV=1)

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 489/528 (92.61%), Postives = 493/528 (93.37%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPC LYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCLLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 120

Query: 400 KYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 459
           KYGACPRFTVGVKGQKVSIKFQVPS+CEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS
Sbjct: 121 KYGACPRFTVGVKGQKVSIKFQVPSNCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 180

Query: 460 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFNKLSATN 519
           PVAWQLSLTRPKTQREAGGNEGNSLEIDAGD DLSVLIFHSLVSSDKT            
Sbjct: 181 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDGDLSVLIFHSLVSSDKT------------ 240

Query: 520 YEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISSLESM 579
            EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSISSLESM
Sbjct: 241 -EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESM 300

Query: 580 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 639
           GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE
Sbjct: 301 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 360

Query: 640 SNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 699
           SNRPRAVLFEGPP         +   Q GVPLVYVPLEVIMSKYYGESERLLGQVFSLAN
Sbjct: 361 SNRPRAVLFEGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 420

Query: 700 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 759
           ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL
Sbjct: 421 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 480

Query: 760 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL 795
           DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL
Sbjct: 481 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL 515

BLAST of Cp4.1LG10g09850 vs. ExPASy TrEMBL
Match: A0A6J1GED9 (nuclear valosin-containing protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453188 PE=3 SV=1)

HSP 1 Score: 921 bits (2380), Expect = 0.0
Identity = 490/535 (91.59%), Postives = 495/535 (92.52%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSVLERIARTVKAWRIIKSFKLKDSSSVSP    RHFRH PEHLKPGFLSNGREPCFLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPP------KDLSTHADLEEIAKKERQRINEQL 399
           AIVASMFGVGAMEIAYAEAEESA TPPPPP      KDLSTHADLEEIAKKERQRINEQL
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPPPKDLSTHADLEEIAKKERQRINEQL 120

Query: 400 IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML 459
           IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML
Sbjct: 121 IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML 180

Query: 460 LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFN 519
           LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT      
Sbjct: 181 LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKT------ 240

Query: 520 KLSATNYEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSI 579
                  EIEFLK+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQLPDPEKSI
Sbjct: 241 -------EIEFLKQGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSI 300

Query: 580 SSLESMGVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG 639
           SSLESMGVKVYGLDE HVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG
Sbjct: 301 SSLESMGVKVYGLDEQHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHG 360

Query: 640 TRRKFESNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQ 699
           TRRKFESNRPRAVLFEGPP         +   Q GVPL+YVPLEVIMSKYYGESERLLGQ
Sbjct: 361 TRRKFESNRPRAVLFEGPPGTGKTSSARVIANQAGVPLLYVPLEVIMSKYYGESERLLGQ 420

Query: 700 VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT 759
           VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT
Sbjct: 421 VFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT 480

Query: 760 NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGLI 796
           NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL+
Sbjct: 481 NRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGLV 522

BLAST of Cp4.1LG10g09850 vs. ExPASy TrEMBL
Match: A0A1S3AZS0 (katanin p60 ATPase-containing subunit A1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484638 PE=3 SV=1)

HSP 1 Score: 913 bits (2360), Expect = 0.0
Identity = 480/600 (80.00%), Postives = 520/600 (86.67%), Query Frame = 0

Query: 280 MSVLERIARTVKAWRIIKSFKLKDSSSVSP----RHFRHHPEHLKPGFLSNGREPCFLYP 339
           MSV  RI+RT +AWRI+ SFKLKDS S S     R F H PEHLKPG LSNGREPCFLYP
Sbjct: 1   MSVFNRISRTARAWRILASFKLKDSPSASRSFYLRQFGHQPEHLKPGLLSNGREPCFLYP 60

Query: 340 AIVASMFGVGAMEIAYAEAEESAGTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 399
           AIVA M GVGAMEIAYAEAEESA TPPPP KDLSTHAD+E++AKKER RI EQL RNKG 
Sbjct: 61  AIVAGMLGVGAMEIAYAEAEESAATPPPP-KDLSTHADMEDVAKKERLRIIEQLNRNKGT 120

Query: 400 KYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 459
           KYGACPRFTVGVKGQKVSIKFQVP SCEVSHL+ANLVSNLGLKVEE AGGSDMLLRAW S
Sbjct: 121 KYGACPRFTVGVKGQKVSIKFQVPPSCEVSHLIANLVSNLGLKVEESAGGSDMLLRAWDS 180

Query: 460 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTVSANFNKLSATN 519
           PVAWQL+L+RPK+QR AG N+GNS+E+DA D DL+VLIFHSLV+SDKTVSAN        
Sbjct: 181 PVAWQLTLSRPKSQRVAGENKGNSIEMDAEDGDLTVLIFHSLVTSDKTVSANVK------ 240

Query: 520 YEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKSREGSTQLPDPEKSISSLESM 579
            EIEF+K+GSLST+ELDSLVSVLQLAGGRLGESRS ERKSREGSTQ+P  EKSISSLE+M
Sbjct: 241 -EIEFIKQGSLSTKELDSLVSVLQLAGGRLGESRSSERKSREGSTQMPSSEKSISSLEAM 300

Query: 580 GVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFE 639
           GVKVYGLDEPHVNS++NEISWDNIAGYDQQK EIED+IL+TLHNPELFD+IAHGTRR+FE
Sbjct: 301 GVKVYGLDEPHVNSTENEISWDNIAGYDQQKREIEDSILMTLHNPELFDDIAHGTRRRFE 360

Query: 640 SNRPRAVLFEGPPV--------ISILQNGVPLVYVPLEVIMSKYYGESERLLGQVFSLAN 699
           SNRPRAVLFEGPP         +   Q GVPLVYVPLEVIMSKYYGESERLLG+VFSLAN
Sbjct: 361 SNRPRAVLFEGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGKVFSLAN 420

Query: 700 ELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDL 759
           ELSTGAIIFLDEVDSFAI+RDSE+HEATRRVLSVLLRQIDGFEQDRKV+VIAATNRKQDL
Sbjct: 421 ELSTGAIIFLDEVDSFAISRDSEIHEATRRVLSVLLRQIDGFEQDRKVIVIAATNRKQDL 480

Query: 760 DPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGL------------ 819
           DPALISRFDLMITFGLPDERNR+EIA QYAKQLTKPEL EFA+NT+G+            
Sbjct: 481 DPALISRFDLMITFGLPDERNREEIAVQYAKQLTKPELKEFARNTDGMSGRDIRDICQQA 540

Query: 820 -------ILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 848
                  I+RGKVSKTGEHGILPP++EYIECAM RRKALQT+DDH+IKD NIRTKKTQ A
Sbjct: 541 ERSWASKIIRGKVSKTGEHGILPPIEEYIECAMKRRKALQTIDDHEIKDPNIRTKKTQLA 592

BLAST of Cp4.1LG10g09850 vs. TAIR 10
Match: AT4G04180.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 542.0 bits (1395), Expect = 8.8e-154
Identity = 313/626 (50.00%), Postives = 415/626 (66.29%), Query Frame = 0

Query: 280 MSVLERIARTVKAWR--IIKSFKLKDSSSVSPRHFR---HHPEHLKPGFLSN-------- 339
           M++L+++ R  +AWR  +  S  +  S S   R  R   HH    +     N        
Sbjct: 1   MAILDKLFRASRAWRGSLSHSKSMVPSQSPRARELRRCFHHGNFEQSNSKVNQVLRSCST 60

Query: 340 -GREPCF-LYPAIVASMFGVGAMEIAYAEAEES---AGTPPPPPKDLSTHADLE------ 399
               P F + PA++ ++F VG + +AYAE++E+     + P  P D ++ A ++      
Sbjct: 61  LNDSPYFSMAPAVLGALFSVGVIGVAYAESDEANNDKSSAPIDPNDETSSAPIDPPPNYV 120

Query: 400 EIAKKERQRINEQLIRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNL 459
           +IAKKER R+ E+LI++KG +YG+ PRF V V+GQK+++KFQVPS+CEV+ L++N+ S L
Sbjct: 121 DIAKKERARV-EELIQSKGTQYGSYPRFNVAVRGQKITLKFQVPSTCEVAQLISNIGSQL 180

Query: 460 GLKVEERAGGSDMLLRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFH 519
           G+KV +R GGSDMLLRAW +PVAWQ++L   + +++ G +E      D  D+DL +LIF 
Sbjct: 181 GVKVSDRTGGSDMLLRAWDNPVAWQITLRSVENKKKLGESE------DDSDDDLCILIFG 240

Query: 520 SLVSSDKTVSANFNKLSATNYEIEFLKRGSLSTEELDSLVSVLQLAGGRLGESRSLERKS 579
           SL++SDK              E+EF+K+GSL+TEEL++ VS L +AG + G+++      
Sbjct: 241 SLLTSDKV-------------EVEFIKKGSLTTEELEAFVSALGVAGTKAGQNKG---SG 300

Query: 580 REGSTQLPDPEKSISSLESMGVKVYGLDEPHVNSSKNEISWDNIAGYDQQKSEIEDTILL 639
             GST+    +KSIS LESMGV++YG+++P  + S +EISWDNIAGYDQQK EIEDTIL+
Sbjct: 301 SRGSTRDSSTDKSISQLESMGVRIYGVNKPLGDDSMDEISWDNIAGYDQQKREIEDTILM 360

Query: 640 TLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP--------VISILQNGVPLVYVPLEVI 699
            LH+PE++D+I  GTR KFESNRPRAVLFEGPP         +   Q G+PL+YVPLE +
Sbjct: 361 ALHSPEVYDDIVRGTRSKFESNRPRAVLFEGPPGTGKTSCARVIANQAGIPLLYVPLEAV 420

Query: 700 MSKYYGESERLLGQVFSLANELSTGAIIFLDEVDSFAIARDSEMHEATRRVLSVLLRQID 759
           MSKYYGESERLLG VFS ANEL  GAIIFLDE+D+FAI+RDSEMHEATRRVLSVLLRQID
Sbjct: 421 MSKYYGESERLLGAVFSQANELPDGAIIFLDEIDAFAISRDSEMHEATRRVLSVLLRQID 480

Query: 760 GFEQDRKVVVIAATNRKQDLDPALISRFDLMITFGLPDERNRQEIAAQYAKQLTKPELNE 819
           GFEQ++KVVVIAATNRKQDLDPALISRFD MI F LPD + RQEI AQYAKQL+KPEL +
Sbjct: 481 GFEQEKKVVVIAATNRKQDLDPALISRFDSMIMFDLPDLQTRQEIIAQYAKQLSKPELVQ 540

Query: 820 FAKNTEG-----------------------------LILRGKVSKTGEHGILPPLQEYIE 845
            A+ TE                              LI R K     +   LPP+QEY+E
Sbjct: 541 LAQATEAMSGRDIRDVCQGAERTWASKIINLYIVGQLIRRAKAGGEEQKITLPPIQEYLE 600

BLAST of Cp4.1LG10g09850 vs. TAIR 10
Match: AT4G22190.1 (unknown protein; Has 283 Blast hits to 154 proteins in 44 species: Archae - 0; Bacteria - 2; Metazoa - 24; Fungi - 12; Plants - 48; Viruses - 0; Other Eukaryotes - 197 (source: NCBI BLink). )

HSP 1 Score: 194.9 bits (494), Expect = 2.6e-49
Identity = 145/278 (52.16%), Postives = 172/278 (61.87%), Query Frame = 0

Query: 11  QPLSPIAGGRRSGSFPTS-PEFEFWMVRNPSFPQ--PNLLSADELFEDGVLLPLHLVSSQ 70
           + LSP    RR  S  ++ PEFEFW + N SFPQ   +LLSADELF DGVLLPL L+S +
Sbjct: 62  ETLSPCGSQRRRSSCDSNPPEFEFWRLTNSSFPQADSDLLSADELFHDGVLLPLDLLSVK 121

Query: 71  SLSLSLSSDPN-KKSDPEPSPSEPDPCDGPK--LTPN-SVESGSSLTSSKRW-SIFKKSE 130
           S    L SDPN  + DP+PSPS        K  L P    E     T SKRW  IF+KSE
Sbjct: 122 S---ELQSDPNIAECDPDPSPSTGSLITEQKSDLEPGLGSELTRETTVSKRWRDIFRKSE 181

Query: 131 KKNVAANQEDRVKEKKKEKK-------SGNGSTSAELNINIWPFSRSRSAGNAYTRPKMF 190
            K     ++++VKE KKEKK       SG+GS  AELNINIWPFSRSRSAGN  TRP+M 
Sbjct: 182 TK--PPGKKEKVKENKKEKKKTGSGPSSGSGS-GAELNINIWPFSRSRSAGNNVTRPRMS 241

Query: 191 AGTQPGSRKVNSAPCSRSNSTGESKSRKWPSSPSRAGVHLGRSSPVWQVRRGGSAAKTSE 250
            G  P +RKV+SAPCSRSNSTGESKSRKWPSSPSR GVHLGR+SPVWQVRRGG A     
Sbjct: 242 FGA-PTTRKVSSAPCSRSNSTGESKSRKWPSSPSRNGVHLGRNSPVWQVRRGGGAPVGKT 301

Query: 251 TFSRNAEKAARKEPTDNNRSKATATAAASRKNKRTVPV 274
                     ++E  +  + K    +  ++     VP+
Sbjct: 302 IPEPMGRVVGKREIPETRKGKTVIESNKAKVLNLNVPM 332

BLAST of Cp4.1LG10g09850 vs. TAIR 10
Match: AT5G58290.1 (regulatory particle triple-A ATPase 3 )

HSP 1 Score: 106.7 bits (265), Expect = 9.4e-23
Identity = 75/195 (38.46%), Postives = 103/195 (52.82%), Query Frame = 0

Query: 589 SSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP 648
           S K ++S+++I G D QK EI + + L L + EL+ +I          + PR VL  GPP
Sbjct: 147 SEKPDVSYNDIGGCDIQKQEIREAVELPLTHHELYKQIG--------IDPPRGVLLYGPP 206

Query: 649 ------VISILQNGVPLVYVPL--EVIMSKYYGESERLLGQVFSLANELSTGAIIFLDEV 708
                 +   + N     ++ +     + KY GE  R++  VF LA E +  AIIF+DEV
Sbjct: 207 GTGKTMLAKAVANHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKE-NAPAIIFIDEV 266

Query: 709 DSFAIARDSEMHEATR---RVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALI--SRF 768
           D+ A AR      A R   R+L  LL Q+DGF+Q   V VI ATNR   LDPAL+   R 
Sbjct: 267 DAIATARFDAQTGADREVQRILMELLNQMDGFDQTVNVKVIMATNRADTLDPALLRPGRL 326

Query: 769 DLMITFGLPDERNRQ 771
           D  I F LPD R ++
Sbjct: 327 DRKIEFPLPDRRQKR 332

BLAST of Cp4.1LG10g09850 vs. TAIR 10
Match: AT5G03340.1 (ATPase, AAA-type, CDC48 protein )

HSP 1 Score: 105.1 bits (261), Expect = 2.7e-22
Identity = 73/218 (33.49%), Postives = 110/218 (50.46%), Query Frame = 0

Query: 592 NEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP--- 651
           +E+ +D++ G  +Q ++I + + L L +P+LF  I            P+ +L  GPP   
Sbjct: 202 DEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIG--------VKPPKGILLYGPPGSG 261

Query: 652 ---VISILQN--GVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIFLDEVDSF 711
              +   + N  G     +    IMSK  GESE  L + F  A E +  +IIF+DE+DS 
Sbjct: 262 KTLIARAVANETGAFFFCINGPEIMSKLAGESESNLRKAFEEA-EKNAPSIIFIDEIDSI 321

Query: 712 AIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPAL--ISRFDLMITF 771
           A  R+    E  RR++S LL  +DG +    V+V+ ATNR   +DPAL    RFD  I  
Sbjct: 322 APKREKTNGEVERRIVSQLLTLMDGLKSRAHVIVMGATNRPNSIDPALRRFGRFDREIDI 381

Query: 772 GLPDERNRQEIAAQYAKQLTKPE---LNEFAKNTEGLI 797
           G+PDE  R E+   + K +   E   L   +K+T G +
Sbjct: 382 GVPDEIGRLEVLRIHTKNMKLAEDVDLERISKDTHGYV 410

BLAST of Cp4.1LG10g09850 vs. TAIR 10
Match: AT3G53230.1 (ATPase, AAA-type, CDC48 protein )

HSP 1 Score: 104.8 bits (260), Expect = 3.6e-22
Identity = 73/218 (33.49%), Postives = 110/218 (50.46%), Query Frame = 0

Query: 592 NEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP--- 651
           +E+ +D++ G  +Q ++I + + L L +P+LF  I            P+ +L  GPP   
Sbjct: 203 DEVGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIG--------VKPPKGILLYGPPGSG 262

Query: 652 ---VISILQN--GVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIFLDEVDSF 711
              +   + N  G     +    IMSK  GESE  L + F  A E +  +IIF+DE+DS 
Sbjct: 263 KTLIARAVANETGAFFFCINGPEIMSKLAGESESNLRKAFEEA-EKNAPSIIFIDEIDSI 322

Query: 712 AIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPAL--ISRFDLMITF 771
           A  R+    E  RR++S LL  +DG +    V+V+ ATNR   +DPAL    RFD  I  
Sbjct: 323 APKREKTHGEVERRIVSQLLTLMDGLKSRAHVIVMGATNRPNSIDPALRRFGRFDREIDI 382

Query: 772 GLPDERNRQEIAAQYAKQLTKPE---LNEFAKNTEGLI 797
           G+PDE  R E+   + K +   E   L   +K+T G +
Sbjct: 383 GVPDEIGRLEVLRIHTKNMKLAEDVDLERVSKDTHGYV 411