Cp4.1LG10g09730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g09730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description1-aminocyclopropane-1-carboxylate oxidase
LocationCp4.1LG10: 4336744 .. 4338174 (-)
RNA-Seq ExpressionCp4.1LG10g09730
SyntenyCp4.1LG10g09730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGATGGATTTCCCTGTTATCAACATGGAGAAGCTTAATGGCGACGAGAGAGGCGCTGTGATGGACCAAATCAACGACGCCTGTCAAAACTGGGGCTTCTTCGAGGTATATTGTTATGAGATTACACGAATAAGCTTCTGGGGTTGATTTATTTTTTGTTAATTATTTCATATAGTTGTTGAATCATGGGATATCGCACGAGCTGATGGACAAGGTGGAGAAGCTGACTAAGGAGCATTACAGGAAGCGTATGGAGCAGAGGTTCAAAGAAATGGTGGCTAGTAAGGGGCTGGATTCAGTGGAGACTGAAAATAACGACACCGATTGGGAAAGCACCTTCTTTCTACGCCATCTTCCCAGTTCTAACATGTCCCAAATCCCTGATTTGGACCAGGATTACAGGGAGGTCATGAAGGAATTCGCAGTGGAATTGGAGAAGCTAGCAGAGCAAATTCTGGACTTGCTGTGCCACAATCTTGGGCTTGAGAAAGGGTACTTGAAGAAGGCCTTTTACGGGTCCAAAGGCCCCAATTTTGGAAGTAAAATCAGCAATTACCCTCCCTGTCCAAAACCTGACCTTATTAAGGGTCTCAGAGCCCACACCGACGCTGGTGGCCTTATTCTCCTCTTTCAAGACCACAAAGTCAGTGGCCTTCAGCTCCTTAAAGACGGCAAATGGGTTGATGTCCCACCAATGCATCACTCCATTGTCATCAATTTAGGCGACCAGCTCGAAGTATGTGCACAAAAAATGTTATTTGATTATGTTTTGTATAACTTATTTGATTATTTATTTGATATATATAGGTAATTACAAATGGAAAATACAAGAGTGTAATGCACAGAGTGATAGCGCAGACAGATGGGAACAGAATGTCGATTGCATCATTCTACAATCCTGGAGACGACGCCGTAATATACCCGGCGCCGGCGCTGGTGGAGGGAGAGGAAGATAAGGGCAAGCTTTACCCAAAATTTGTGTTCGATGATTACATGAAGCTGTACGTGGGGGTGAAGTTTGAGGCAAAAGAGCCGAGGTTTGAGGCCATGAAGGCTATGGAATCGACTAACCCAATTGCGACTGTTTGAGAACTCCATTTTCCAATAACCCATTAAGACATGGCATTGAAATAGAAAGCTTTTTATTATGTTATCAGAAACAAAATTTAGTGGTACCATAGTGACGATGTTTCGGTGTTGGGGAACAGGGCTTGAAGTTTTTTCTTTTTAAGATATGATAGATGTTTCAAGATTAAATGTTTAAATAATGGATATGTGGACACTTTTCAGATGTGTTGTCTTGTCTTGGTTTCATATAAACTTGTAAGGGATGCTTCATTCACCTTTTCTACGGAAGTGAGATCTCACAATTGACCCTCTTGGACCCAGTGACTGACTCCGTGAGATCTCACAATTGACCCTCTTGGA

mRNA sequence

ATGGAGATGGATTTCCCTGTTATCAACATGGAGAAGCTTAATGGCGACGAGAGAGGCGCTGTGATGGACCAAATCAACGACGCCTGTCAAAACTGGGGCTTCTTCGAGTTGTTGAATCATGGGATATCGCACGAGCTGATGGACAAGGTGGAGAAGCTGACTAAGGAGCATTACAGGAAGCGTATGGAGCAGAGGTTCAAAGAAATGGTGGCTAGTAAGGGGCTGGATTCAGTGGAGACTGAAAATAACGACACCGATTGGGAAAGCACCTTCTTTCTACGCCATCTTCCCAGTTCTAACATGTCCCAAATCCCTGATTTGGACCAGGATTACAGGGAGGTCATGAAGGAATTCGCAGTGGAATTGGAGAAGCTAGCAGAGCAAATTCTGGACTTGCTGTGCCACAATCTTGGGCTTGAGAAAGGGTACTTGAAGAAGGCCTTTTACGGGTCCAAAGGCCCCAATTTTGGAAGTAAAATCAGCAATTACCCTCCCTGTCCAAAACCTGACCTTATTAAGGGTCTCAGAGCCCACACCGACGCTGGTGGCCTTATTCTCCTCTTTCAAGACCACAAAGTCAGTGGCCTTCAGCTCCTTAAAGACGGCAAATGGGTTGATGTCCCACCAATGCATCACTCCATTGTCATCAATTTAGGCGACCAGCTCGAAGTAATTACAAATGGAAAATACAAGAGTGTAATGCACAGAGTGATAGCGCAGACAGATGGGAACAGAATGTCGATTGCATCATTCTACAATCCTGGAGACGACGCCGTAATATACCCGGCGCCGGCGCTGGTGGAGGGAGAGGAAGATAAGGGCAAGCTTTACCCAAAATTTGTGTTCGATGATTACATGAAGCTGTACGTGGGGGTGAAGTTTGAGGCAAAAGAGCCGAGGTTTGAGGCCATGAAGGCTATGGAATCGACTAACCCAATTGCGACTGTTTGAGAACTCCATTTTCCAATAACCCATTAAGACATGGCATTGAAATAGAAAGCTTTTTATTATGTTATCAGAAACAAAATTTAGTGGTACCATAGTGACGATGTTTCGGTGTTGGGGAACAGGGCTTGAAGTTTTTTCTTTTTAAGATATGATAGATGTTTCAAGATTAAATGTTTAAATAATGGATATGTGGACACTTTTCAGATGTGTTGTCTTGTCTTGGTTTCATATAAACTTGTAAGGGATGCTTCATTCACCTTTTCTACGGAAGTGAGATCTCACAATTGACCCTCTTGGACCCAGTGACTGACTCCGTGAGATCTCACAATTGACCCTCTTGGA

Coding sequence (CDS)

ATGGAGATGGATTTCCCTGTTATCAACATGGAGAAGCTTAATGGCGACGAGAGAGGCGCTGTGATGGACCAAATCAACGACGCCTGTCAAAACTGGGGCTTCTTCGAGTTGTTGAATCATGGGATATCGCACGAGCTGATGGACAAGGTGGAGAAGCTGACTAAGGAGCATTACAGGAAGCGTATGGAGCAGAGGTTCAAAGAAATGGTGGCTAGTAAGGGGCTGGATTCAGTGGAGACTGAAAATAACGACACCGATTGGGAAAGCACCTTCTTTCTACGCCATCTTCCCAGTTCTAACATGTCCCAAATCCCTGATTTGGACCAGGATTACAGGGAGGTCATGAAGGAATTCGCAGTGGAATTGGAGAAGCTAGCAGAGCAAATTCTGGACTTGCTGTGCCACAATCTTGGGCTTGAGAAAGGGTACTTGAAGAAGGCCTTTTACGGGTCCAAAGGCCCCAATTTTGGAAGTAAAATCAGCAATTACCCTCCCTGTCCAAAACCTGACCTTATTAAGGGTCTCAGAGCCCACACCGACGCTGGTGGCCTTATTCTCCTCTTTCAAGACCACAAAGTCAGTGGCCTTCAGCTCCTTAAAGACGGCAAATGGGTTGATGTCCCACCAATGCATCACTCCATTGTCATCAATTTAGGCGACCAGCTCGAAGTAATTACAAATGGAAAATACAAGAGTGTAATGCACAGAGTGATAGCGCAGACAGATGGGAACAGAATGTCGATTGCATCATTCTACAATCCTGGAGACGACGCCGTAATATACCCGGCGCCGGCGCTGGTGGAGGGAGAGGAAGATAAGGGCAAGCTTTACCCAAAATTTGTGTTCGATGATTACATGAAGCTGTACGTGGGGGTGAAGTTTGAGGCAAAAGAGCCGAGGTTTGAGGCCATGAAGGCTATGGAATCGACTAACCCAATTGCGACTGTTTGA

Protein sequence

MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDAGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQTDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPRFEAMKAMESTNPIATV
Homology
BLAST of Cp4.1LG10g09730 vs. ExPASy Swiss-Prot
Match: P54847 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=ACO3 PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 2.6e-164
Identity = 279/320 (87.19%), Postives = 301/320 (94.06%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINM  LNG+ R +V++QINDAC+NWGFFEL+NHGISHELMDKVEKLTKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
            MEQRFKEMVASKGLDSVETE NDTDWESTFFLRHLP SNMS+I DLD++Y++VMKEFA 
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAD 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAE++LDLLC NLGLEKGYLKK FYGSKGPNFG+K+SNYPPCPKP+LIKGLRAHTD
Sbjct: 121 ELEKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQD KVSGL +LKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLHVLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
            DGNRMSIASFYNPG+DAVIYPAPALVEGE++K KLYPKFVFDDYMKLYVG+KF+AKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPR 300

Query: 301 FEAMKAMESTN----PIATV 317
           FEAMKAMESTN    PIATV
Sbjct: 301 FEAMKAMESTNLNMGPIATV 320

BLAST of Cp4.1LG10g09730 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 2.1e-150
Identity = 252/316 (79.75%), Postives = 290/316 (91.77%), Query Frame = 0

Query: 5   FPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRMEQ 64
           FPVI+MEKLNG+ER   M++I DAC+NWGFFEL+NHGISHELMD VE+LTKEHY K MEQ
Sbjct: 4   FPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCMEQ 63

Query: 65  RFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVELEK 124
           RFKEMVA+KGL++V++E ND DWESTFFLRHLP SN+S+IPDL+QD+R+ MKEFA +LEK
Sbjct: 64  RFKEMVATKGLEAVQSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEFAEKLEK 123

Query: 125 LAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDAGGL 184
           LAEQ+LDLLC N+GLEKGYLKKAFYGSKGP FG+K+SNYPPCP+P+LIKGLRAHTDAGG+
Sbjct: 124 LAEQLLDLLCENVGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDAGGI 183

Query: 185 ILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQTDGN 244
           ILLFQD+KVSGLQLLKDG+W+DVPPM HSIVIN+GDQLEVITNGKYKSVMHRVIAQ DGN
Sbjct: 184 ILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMHRVIAQPDGN 243

Query: 245 RMSIASFYNPGDDAVIYPAPALVEGEEDKGK-LYPKFVFDDYMKLYVGVKFEAKEPRFEA 304
           RMSIASFYNPG DAV+YPAPALV+ EED+ K +YPKFVF+DYMKLY G+KF+AKEPRFEA
Sbjct: 244 RMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKFQAKEPRFEA 303

Query: 305 MKAMEST---NPIATV 317
           MKAME+     PIAT+
Sbjct: 304 MKAMENAVNLGPIATI 319

BLAST of Cp4.1LG10g09730 vs. ExPASy Swiss-Prot
Match: Q08507 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 2.6e-148
Identity = 248/317 (78.23%), Postives = 284/317 (89.59%), Query Frame = 0

Query: 4   DFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRME 63
           +FP+IN+EKLNG ER A M+ I DAC+NWGFFEL+NHGI HE+MD VEKLTK HY+K ME
Sbjct: 3   NFPIINLEKLNGSERDATMEMIKDACENWGFFELVNHGIPHEVMDTVEKLTKGHYKKCME 62

Query: 64  QRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVELE 123
           QRFKE+VASKGL++V+ E  D DWESTFFLRHLP SN+S++PDLD +YREVM++FA  LE
Sbjct: 63  QRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPVSNISEVPDLDDEYREVMRDFAKRLE 122

Query: 124 KLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDAGG 183
           KLAE++LDLLC NLGLEKGYLKKAFYGSKGPNFG+K+SNYPPCPKPDLIKGLRAHTDAGG
Sbjct: 123 KLAEELLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGG 182

Query: 184 LILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQTDG 243
           +ILLFQD KVSGLQLLKDG+W+DVPPM HSIV+NLGDQLEVITNGKYKSV+HRVIAQTDG
Sbjct: 183 IILLFQDDKVSGLQLLKDGQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQTDG 242

Query: 244 NRMSIASFYNPGDDAVIYPAPALVEGEEDK--GKLYPKFVFDDYMKLYVGVKFEAKEPRF 303
            RMS+ASFYNPG DAVIYPAP LVE E D+   ++YPKFVFDDYMKLY G+KF+AKEPRF
Sbjct: 243 TRMSLASFYNPGSDAVIYPAPTLVEKEADQECKQVYPKFVFDDYMKLYAGLKFQAKEPRF 302

Query: 304 EAMKAMES---TNPIAT 316
           EAMKA E+   ++PIAT
Sbjct: 303 EAMKAREADVKSDPIAT 319

BLAST of Cp4.1LG10g09730 vs. ExPASy Swiss-Prot
Match: P05116 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=ACO1 PE=2 SV=2)

HSP 1 Score: 523.9 bits (1348), Expect = 1.3e-147
Identity = 246/313 (78.59%), Postives = 283/313 (90.42%), Query Frame = 0

Query: 4   DFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRME 63
           +FP+IN+EKLNGDER   M+ I DAC+NWGFFEL+NHGI HE+MD VEK+TK HY+K ME
Sbjct: 3   NFPIINLEKLNGDERANTMEMIKDACENWGFFELVNHGIPHEVMDTVEKMTKGHYKKCME 62

Query: 64  QRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVELE 123
           QRFKE+VASKGL++V+ E  D DWESTFFLRHLP+SN+SQ+PDLD++YREVM++FA  LE
Sbjct: 63  QRFKELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMRDFAKRLE 122

Query: 124 KLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDAGG 183
           KLAE++LDLLC NLGLEKGYLK AFYGSKGPNFG+K+SNYPPCPKPDLIKGLRAHTDAGG
Sbjct: 123 KLAEELLDLLCENLGLEKGYLKNAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGG 182

Query: 184 LILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQTDG 243
           +ILLFQD KVSGLQLLKD +W+DVPPM HSIV+NLGDQLEVITNGKYKSV+HRVIAQTDG
Sbjct: 183 IILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSVLHRVIAQTDG 242

Query: 244 NRMSIASFYNPGDDAVIYPAPALVEGE-EDKGKLYPKFVFDDYMKLYVGVKFEAKEPRFE 303
            RMS+ASFYNPG DAVIYPA  LVE E E+  ++YPKFVFDDYMKLY G+KF+AKEPRFE
Sbjct: 243 TRMSLASFYNPGSDAVIYPAKTLVEKEAEESTQVYPKFVFDDYMKLYAGLKFQAKEPRFE 302

Query: 304 AMKAMESTNPIAT 316
           AMKAMES +PIA+
Sbjct: 303 AMKAMES-DPIAS 314

BLAST of Cp4.1LG10g09730 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 4.9e-147
Identity = 242/311 (77.81%), Postives = 285/311 (91.64%), Query Frame = 0

Query: 5   FPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRMEQ 64
           FPV+++  +NG+ER A +++INDAC+NWGFFEL+NHG+S EL+D VEK+TK+HY+K MEQ
Sbjct: 4   FPVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQ 63

Query: 65  RFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVELEK 124
           RFKEMVA+KGLD V++E +D DWESTFFLRHLPSSN+S+IPDL+++YR+ MKEFAVELEK
Sbjct: 64  RFKEMVAAKGLDDVQSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFAVELEK 123

Query: 125 LAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDAGGL 184
           LAE++LDLLC NLGLEKGYLKK FYGSKGPNFG+K+SNYPPCPKPDLIKGLRAH+DAGG+
Sbjct: 124 LAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHSDAGGI 183

Query: 185 ILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQTDGN 244
           ILLFQD KVSGLQLLKDG+WVDVPPMHHSIVINLGDQ+EVITNGKYKSVMHRVIAQ+DG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKYKSVMHRVIAQSDGT 243

Query: 245 RMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPRFEAM 304
           RMSIASFYNPG+D+ I PAPA++E + +    YPKFVFDDYMKLY G+KF+AKEPRFEAM
Sbjct: 244 RMSIASFYNPGNDSFISPAPAVLEKKTEDAPTYPKFVFDDYMKLYSGLKFQAKEPRFEAM 303

Query: 305 KAMESTNPIAT 316
           KA EST P+AT
Sbjct: 304 KAKEST-PVAT 313

BLAST of Cp4.1LG10g09730 vs. NCBI nr
Match: XP_023521976.1 (1-aminocyclopropane-1-carboxylate oxidase 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023521977.1 1-aminocyclopropane-1-carboxylate oxidase 3-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023544358.1 1-aminocyclopropane-1-carboxylate oxidase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650 bits (1678), Expect = 8.03e-236
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK
Sbjct: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
           RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV
Sbjct: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD
Sbjct: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
           TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR
Sbjct: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300

Query: 301 FEAMKAMESTNPIATV 316
           FEAMKAMESTNPIATV
Sbjct: 301 FEAMKAMESTNPIATV 316

BLAST of Cp4.1LG10g09730 vs. NCBI nr
Match: XP_023521978.1 (1-aminocyclopropane-1-carboxylate oxidase 3-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 648 bits (1672), Expect = 6.60e-235
Identity = 315/316 (99.68%), Postives = 315/316 (99.68%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK
Sbjct: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
           RMEQRFKEMVASKGLD VETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV
Sbjct: 61  RMEQRFKEMVASKGLDLVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD
Sbjct: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
           TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR
Sbjct: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300

Query: 301 FEAMKAMESTNPIATV 316
           FEAMKAMESTNPIATV
Sbjct: 301 FEAMKAMESTNPIATV 316

BLAST of Cp4.1LG10g09730 vs. NCBI nr
Match: XP_022950853.1 (1-aminocyclopropane-1-carboxylate oxidase 3-like [Cucurbita moschata])

HSP 1 Score: 643 bits (1659), Expect = 6.33e-233
Identity = 313/316 (99.05%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK
Sbjct: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
           RMEQRFKEMVASKGLDSVE+ENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV
Sbjct: 61  RMEQRFKEMVASKGLDSVESENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQILDLLC NLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD
Sbjct: 121 ELEKLAEQILDLLCQNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
           TDGNRMSIASFYNPGDDAVIYPAPALVEGE DKGKLYPKFVFDDYMKLYVGVKFEAKEPR
Sbjct: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEGDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300

Query: 301 FEAMKAMESTNPIATV 316
           FEAMKAMESTNPIATV
Sbjct: 301 FEAMKAMESTNPIATV 316

BLAST of Cp4.1LG10g09730 vs. NCBI nr
Match: KAG6603806.1 (1-aminocyclopropane-1-carboxylate oxidase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 642 bits (1657), Expect = 1.28e-232
Identity = 312/316 (98.73%), Postives = 315/316 (99.68%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK
Sbjct: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
           RMEQRFKEMVASKGLDSVE+ENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV
Sbjct: 61  RMEQRFKEMVASKGLDSVESENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD
Sbjct: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPM+HSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMNHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
           TDGNRMSIASFYNPGDDAVIYPAPALVEGE DKGKLYPKFVFDDYMKLYVG+KFEAKEPR
Sbjct: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEGDKGKLYPKFVFDDYMKLYVGLKFEAKEPR 300

Query: 301 FEAMKAMESTNPIATV 316
           FEAMKAMESTNPIATV
Sbjct: 301 FEAMKAMESTNPIATV 316

BLAST of Cp4.1LG10g09730 vs. NCBI nr
Match: XP_022979040.1 (1-aminocyclopropane-1-carboxylate oxidase 3-like [Cucurbita maxima])

HSP 1 Score: 641 bits (1653), Expect = 5.20e-232
Identity = 309/316 (97.78%), Postives = 315/316 (99.68%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEK TKEHYRK
Sbjct: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKQTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
           RMEQ+FKEMVASKGLDSV++E+NDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV
Sbjct: 61  RMEQKFKEMVASKGLDSVDSEHNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFG+K+SNYPPCPKPDLIKGLRAHTD
Sbjct: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
           TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR
Sbjct: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300

Query: 301 FEAMKAMESTNPIATV 316
           FEAMKAMESTNPIATV
Sbjct: 301 FEAMKAMESTNPIATV 316

BLAST of Cp4.1LG10g09730 vs. ExPASy TrEMBL
Match: A0A6J1GG16 (1-aminocyclopropane-1-carboxylate oxidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111453828 PE=3 SV=1)

HSP 1 Score: 643 bits (1659), Expect = 3.07e-233
Identity = 313/316 (99.05%), Postives = 314/316 (99.37%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK
Sbjct: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
           RMEQRFKEMVASKGLDSVE+ENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV
Sbjct: 61  RMEQRFKEMVASKGLDSVESENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQILDLLC NLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD
Sbjct: 121 ELEKLAEQILDLLCQNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
           TDGNRMSIASFYNPGDDAVIYPAPALVEGE DKGKLYPKFVFDDYMKLYVGVKFEAKEPR
Sbjct: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEGDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300

Query: 301 FEAMKAMESTNPIATV 316
           FEAMKAMESTNPIATV
Sbjct: 301 FEAMKAMESTNPIATV 316

BLAST of Cp4.1LG10g09730 vs. ExPASy TrEMBL
Match: A0A6J1IS25 (1-aminocyclopropane-1-carboxylate oxidase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478800 PE=3 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 2.52e-232
Identity = 309/316 (97.78%), Postives = 315/316 (99.68%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEK TKEHYRK
Sbjct: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKQTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
           RMEQ+FKEMVASKGLDSV++E+NDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV
Sbjct: 61  RMEQKFKEMVASKGLDSVDSEHNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFG+K+SNYPPCPKPDLIKGLRAHTD
Sbjct: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
           TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR
Sbjct: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300

Query: 301 FEAMKAMESTNPIATV 316
           FEAMKAMESTNPIATV
Sbjct: 301 FEAMKAMESTNPIATV 316

BLAST of Cp4.1LG10g09730 vs. ExPASy TrEMBL
Match: A0A6J1EEI3 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita moschata OX=3662 GN=LOC111433496 PE=3 SV=1)

HSP 1 Score: 588 bits (1516), Expect = 4.04e-211
Identity = 281/320 (87.81%), Postives = 305/320 (95.31%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNG++RGA M+QINDAC+NWGFFEL+NHGISHELMD+VEKLTKEHYR+
Sbjct: 17  MEMDFPVINMEKLNGEDRGAAMEQINDACENWGFFELVNHGISHELMDEVEKLTKEHYRQ 76

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
            MEQ+F +MVAS+GLDSV+TE NDTDWESTFFLRHLP SNMS+IPDLD+DYR+VMKEFAV
Sbjct: 77  CMEQKFNDMVASRGLDSVDTEINDTDWESTFFLRHLPVSNMSEIPDLDEDYRKVMKEFAV 136

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQ+LDLLC NLGLEKGYLKKAFYGSKGPNFG+K+SNYPPCPKP+LIKGLRAHTD
Sbjct: 137 ELEKLAEQLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 196

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRV AQ
Sbjct: 197 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVKAQ 256

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
            DGNRMS+ASFYNPGDDAVIYPAPALVEGEE+  KLYPKFVF+DYMKLY+G+KF+AKEPR
Sbjct: 257 PDGNRMSLASFYNPGDDAVIYPAPALVEGEEESSKLYPKFVFNDYMKLYMGLKFQAKEPR 316

Query: 301 FEAMKAMESTN----PIATV 316
           FEAMKA ESTN    PIATV
Sbjct: 317 FEAMKATESTNLKMGPIATV 336

BLAST of Cp4.1LG10g09730 vs. ExPASy TrEMBL
Match: A0A6J1KXP7 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita maxima OX=3661 GN=LOC111497285 PE=3 SV=1)

HSP 1 Score: 586 bits (1510), Expect = 3.32e-210
Identity = 280/320 (87.50%), Postives = 305/320 (95.31%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINMEKLNG++RGA M++INDAC+NWGFFEL+NHGISHELMD+VEKLTKEHYRK
Sbjct: 17  MEMDFPVINMEKLNGEDRGAAMEKINDACENWGFFELVNHGISHELMDEVEKLTKEHYRK 76

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
            MEQ+FK+MVAS+GLDSV+TE NDTDWESTFFLRHLP SNMS+IPDLD+DY++VMKEFAV
Sbjct: 77  CMEQKFKDMVASRGLDSVDTEINDTDWESTFFLRHLPVSNMSEIPDLDEDYKKVMKEFAV 136

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAEQ+LDLLC NLGLEKGYLKKAFYGSKGPNFG+K+SNYPPCPKP+LI+GLRAHTD
Sbjct: 137 ELEKLAEQLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPELIEGLRAHTD 196

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRV AQ
Sbjct: 197 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVKAQ 256

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
            DGNRMS+ASFYNPGDDAVIYPAPALVEGEE   KLYPKFVF+DYMKLYVG+KF+AKEPR
Sbjct: 257 PDGNRMSLASFYNPGDDAVIYPAPALVEGEEASSKLYPKFVFNDYMKLYVGLKFQAKEPR 316

Query: 301 FEAMKAMESTN----PIATV 316
           FEAMKA +STN    PIATV
Sbjct: 317 FEAMKATDSTNLQMGPIATV 336

BLAST of Cp4.1LG10g09730 vs. ExPASy TrEMBL
Match: A0A1S3B069 (1-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103484645 PE=3 SV=1)

HSP 1 Score: 581 bits (1497), Expect = 1.76e-208
Identity = 281/320 (87.81%), Postives = 302/320 (94.38%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           MEMDFPVINM  LNG+ R +V++QINDAC+NWGFFEL+NHGISHELMDKVEKLTKEHYRK
Sbjct: 1   MEMDFPVINMNNLNGESRVSVLNQINDACENWGFFELVNHGISHELMDKVEKLTKEHYRK 60

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
            MEQRFKEMVASKGLDSVETE NDTDWESTFFLRHLP SNMS+I DLD++Y++VMKEFA 
Sbjct: 61  CMEQRFKEMVASKGLDSVETEINDTDWESTFFLRHLPVSNMSEIGDLDEEYKKVMKEFAD 120

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
           ELEKLAE++LDLLC NLGLEKGYLKK FYGSKGPNFG+K+SNYPPCPKP+LIKGLRAHTD
Sbjct: 121 ELEKLAEEVLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPELIKGLRAHTD 180

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQD KVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ
Sbjct: 181 AGGLILLFQDDKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
            DGNRMSIASFYNPG+DAVIYPAPALVEGE++K KLYPKFVFDDYMKLYVG+KF+AKEPR
Sbjct: 241 EDGNRMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPR 300

Query: 301 FEAMKAMESTN----PIATV 316
           FEAMKAMESTN    PIATV
Sbjct: 301 FEAMKAMESTNLNMGPIATV 320

BLAST of Cp4.1LG10g09730 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 492.3 bits (1266), Expect = 3.0e-139
Identity = 230/319 (72.10%), Postives = 277/319 (86.83%), Query Frame = 0

Query: 5   FPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRMEQ 64
           FP+IN+EKLNG+ER   M++I DAC+NWGFFE +NHGIS EL+DKVEK+TKEHY+K ME+
Sbjct: 4   FPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCMEE 63

Query: 65  RFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVELEK 124
           RFKE + ++GLDS+ +E ND DWESTF+L+HLP SN+S +PDLD DYR +MK+FA ++EK
Sbjct: 64  RFKESIKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDFAGKIEK 123

Query: 125 LAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDAGGL 184
           L+E++LDLLC NLGLEKGYLKK FYGSK P FG+K+SNYPPCP PDL+KGLRAHTDAGG+
Sbjct: 124 LSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDAGGI 183

Query: 185 ILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQTDG- 244
           ILLFQD KVSGLQLLKDG+WVDVPP+ HSIV+NLGDQLEVITNGKYKSV HRV++QTDG 
Sbjct: 184 ILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEHRVLSQTDGE 243

Query: 245 NRMSIASFYNPGDDAVIYPAPALV--EGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPRF 304
            RMSIASFYNPG D+VI+PAP L+  E E++K + YP+FVF+DYMKLY  VKF+AKEPRF
Sbjct: 244 GRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAVKFQAKEPRF 303

Query: 305 EAMKAMEST-----NPIAT 316
           EAMKAME+T      P+AT
Sbjct: 304 EAMKAMETTVANNVGPLAT 322

BLAST of Cp4.1LG10g09730 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 473.4 bits (1217), Expect = 1.4e-133
Identity = 224/319 (70.22%), Postives = 262/319 (82.13%), Query Frame = 0

Query: 1   MEMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRK 60
           M + FPVI++ KLNG+ER   M  I+DACQNWGFFEL+NHG+ ++LMD +E++TKEHY+K
Sbjct: 3   MNIKFPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKK 62

Query: 61  RMEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAV 120
            MEQ+FKEM+ SKGLD++ETE  D DWESTF+L HLP SN+  IPD+  +YR  MK+F  
Sbjct: 63  HMEQKFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGK 122

Query: 121 ELEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTD 180
            LE LAE++LDLLC NLGLEKGYLKK F+G+ GP F +K+SNYPPCPKP++IKGLRAHTD
Sbjct: 123 RLEILAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTD 182

Query: 181 AGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 240
           AGGLILLFQD KVSGLQLLKDG WVDVPP+ HSIVINLGDQLEVITNGKYKSVMHRV+ Q
Sbjct: 183 AGGLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRVMTQ 242

Query: 241 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPR 300
            +GNRMSIASFYNPG DA I PA +LV    DK   YP FVFDDYMKLY G+KF+AKEPR
Sbjct: 243 KEGNRMSIASFYNPGSDAEISPATSLV----DKDSKYPSFVFDDYMKLYAGLKFQAKEPR 302

Query: 301 FEAMKAMEST---NPIATV 317
           FEAMK  E+    NP+A V
Sbjct: 303 FEAMKNAEAAADLNPVAVV 317

BLAST of Cp4.1LG10g09730 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 465.3 bits (1196), Expect = 3.9e-131
Identity = 214/317 (67.51%), Postives = 260/317 (82.02%), Query Frame = 0

Query: 3   MDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRM 62
           M FPV+++ KLNG+ER   M  IN+AC+NWGFFE++NHG+ H+LMDK+EK+TK+HY+   
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 63  EQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVEL 122
           EQ+F +M+ SKGLD++ETE  D DWESTF++RHLP SN++ I D+  +YR  MK+F   L
Sbjct: 65  EQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAMKDFGKRL 124

Query: 123 EKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDAG 182
           E LAE +LDLLC NLGLEKGYLKK F+G+KGP FG+K+SNYPPCPKP++IKGLRAHTDAG
Sbjct: 125 ENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDAG 184

Query: 183 GLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKSVMHRVIAQTD 242
           G+ILLFQD KVSGLQLLKDG W+DVPP++HSIVINLGDQLEVITNGKYKSV+HRV+ Q +
Sbjct: 185 GIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKYKSVLHRVVTQQE 244

Query: 243 GNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGVKFEAKEPRFE 302
           GNRMS+ASFYNPG DA I PA +LVE + +    YP FVFDDYMKLY GVKF+ KEPRF 
Sbjct: 245 GNRMSVASFYNPGSDAEISPATSLVEKDSE----YPSFVFDDYMKLYAGVKFQPKEPRFA 304

Query: 303 AMK---AMESTNPIATV 317
           AMK   A+   NP A V
Sbjct: 305 AMKNASAVTELNPTAAV 317

BLAST of Cp4.1LG10g09730 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 312.8 bits (800), Expect = 3.2e-85
Identity = 147/308 (47.73%), Postives = 211/308 (68.51%), Query Frame = 0

Query: 2   EMDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKR 61
           EM+ PVI+  +L+G++R   M  ++ AC  WGFF + NHGI  ELM+KV+K+   HY + 
Sbjct: 8   EMEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEH 67

Query: 62  MEQRFKEMVASKGLDSVETENNDTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKEFAVE 121
           ++++F +    K L   E + +D DWES+FF+ H P+SN+ QIP++ ++  + M E+  +
Sbjct: 68  LKEKFYQSEMVKALS--EGKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDEYVCQ 127

Query: 122 LEKLAEQILDLLCHNLGLEKGYLKKAFYGSKGPNFGSKISNYPPCPKPDLIKGLRAHTDA 181
           L K AE++  L+C NLGL++  +  AF G KGP FG+K++ YP CP+P+L++GLR HTDA
Sbjct: 128 LHKFAERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDA 187

Query: 182 GGLILLFQDHKVSGLQLLKDGKWVDVPP-MHHSIVINLGDQLEVITNGKYKSVMHRVIAQ 241
           GG+ILL QD +V GL+  KDGKWV +PP  +++I +N GDQLE+++NG+YKSV+HRV+  
Sbjct: 188 GGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTV 247

Query: 242 TDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPK-FVFDDYMKLYVGVKFEAKEP 301
             G+R+SIA+FYNP  DA+I PAP L         LYP  + F DY+KLY   KF  K P
Sbjct: 248 KHGSRLSIATFYNPAGDAIISPAPKL---------LYPSGYRFQDYLKLYSTTKFGDKGP 304

Query: 302 RFEAMKAM 308
           R E MK M
Sbjct: 308 RLETMKKM 304

BLAST of Cp4.1LG10g09730 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 2.6e-82
Identity = 154/315 (48.89%), Postives = 212/315 (67.30%), Query Frame = 0

Query: 3   MDFPVINMEKLNGDERGAVMDQINDACQNWGFFELLNHGISHELMDKVEKLTKEHYRKRM 62
           M  PVI+  KLNG+ER   + +I  AC+ WGFF+L+NHGI  EL++KV+KL+ + Y+   
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 63  EQRFKEMVASKGLDSVETENN-----DTDWESTFFLRHLPSSNMSQIPDLDQDYREVMKE 122
           E+ FK     K L+ +  +N+     + DWE  F L      N ++ P    + +E M E
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLL---DHNQNEWP---SNIKETMGE 120

Query: 123 FAVELEKLAEQILDLLCHNLGLEKGYLKKAFY-----GSKGPNFGSKISNYPPCPKPDLI 182
           +  E+ KLA ++++++  NLGL KGY+KKAF      G +   FG+K+S+YPPCP P+L+
Sbjct: 121 YREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELV 180

Query: 183 KGLRAHTDAGGLILLFQDHKVSGLQLLKDGKWVDVPPMHHSIVINLGDQLEVITNGKYKS 242
            GLRAHTDAGG++LLFQD +  GLQ+LKDG+W+DV P+ ++IVIN GDQ+EV++NG+YKS
Sbjct: 181 NGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKS 240

Query: 243 VMHRVIAQTDGNRMSIASFYNPGDDAVIYPAPALVEGEEDKGKLYPKFVFDDYMKLYVGV 302
             HRV+A+ +GNR SIASFYNP   A I   PA V  EE   K YPKFVF DYM +Y   
Sbjct: 241 AWHRVLAREEGNRRSIASFYNPSYKAAI--GPAAVAEEEGSEKKYPKFVFGDYMDVYANQ 300

Query: 303 KFEAKEPRFEAMKAM 308
           KF  KEPRF A+K++
Sbjct: 301 KFMPKEPRFLAVKSL 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P548472.6e-16487.191-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=ACO3 PE=2... [more]
P312372.1e-15079.751-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ... [more]
Q085072.6e-14878.231-aminocyclopropane-1-carboxylate oxidase 3 OS=Petunia hybrida OX=4102 GN=ACO3 P... [more]
P051161.3e-14778.591-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=A... [more]
Q009854.9e-14777.811-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023521976.18.03e-236100.001-aminocyclopropane-1-carboxylate oxidase 3-like isoform X1 [Cucurbita pepo subs... [more]
XP_023521978.16.60e-23599.681-aminocyclopropane-1-carboxylate oxidase 3-like isoform X3 [Cucurbita pepo subs... [more]
XP_022950853.16.33e-23399.051-aminocyclopropane-1-carboxylate oxidase 3-like [Cucurbita moschata][more]
KAG6603806.11.28e-23298.731-aminocyclopropane-1-carboxylate oxidase 3, partial [Cucurbita argyrosperma sub... [more]
XP_022979040.15.20e-23297.781-aminocyclopropane-1-carboxylate oxidase 3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GG163.07e-23399.051-aminocyclopropane-1-carboxylate oxidase 3-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1IS252.52e-23297.781-aminocyclopropane-1-carboxylate oxidase 3-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1EEI34.04e-21187.811-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KXP73.32e-21087.501-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A1S3B0691.76e-20887.811-aminocyclopropane-1-carboxylate oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103484... [more]
Match NameE-valueIdentityDescription
AT1G05010.13.0e-13972.10ethylene-forming enzyme [more]
AT1G12010.11.4e-13370.222-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G62380.13.9e-13167.51ACC oxidase 2 [more]
AT2G19590.13.2e-8547.73ACC oxidase 1 [more]
AT1G77330.12.6e-8248.892-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 6..97
e-value: 9.1E-15
score: 55.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 161..254
e-value: 6.0E-27
score: 94.1
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 3..316
e-value: 4.7E-103
score: 346.8
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 2..306
NoneNo IPR availablePANTHERPTHR47990:SF63ACC OXIDASE 2coord: 2..306
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 3..305
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 153..254
score: 13.595261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g09730.1Cp4.1LG10g09730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009693 ethylene biosynthetic process
biological_process GO:0009835 fruit ripening
molecular_function GO:0009815 1-aminocyclopropane-1-carboxylate oxidase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0046872 metal ion binding