Cp4.1LG10g09700 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG10g09700
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	cytochrome P450 77A1
Location	Cp4.1LG10: 4496040 .. 4497805 (-)
RNA-Seq Expression	Cp4.1LG10g09700
Synteny	Cp4.1LG10g09700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GACGTCGCCATTTATTTTCAACTTTCAATTATCAAACACATTTCTTGATCTCGCCTCGCCTCTGCTATGGACTTACTCGATGCCGTTGTTCTTCTCCTGGCCTTGCTTTTTTTCTCCTTATGGTGGCGTTATTGGTCCGCTACTGGGGGTGGCTCCAAGAATCTCCCTCCTGGGCCACCGGGCTGGCCGATTGTCGGAAATCTCATACAAGTTATTCTCCAACGTCGTCCGTTTATTTTCGTGGCTCGCGATTTACGTGAAAAATATGGTCCCATTTTCACAATGCAAATGGGGCAACGCACACTCATCATTGTGTCTAGTGCGGAGTTGATTCACGAAGCTCTCGTGCAGCGTGGTCCCTTATTTGCCAGCCGTCCTGCTGACTCACCTATTCGTCTAGTCTTCAGCGTTGGAAAATGCGCTGTCAACTCAGCCGAGTATGGTCCCCTATGGCGAACACTCCGCCGTAACTTCGTGACGGAGCTGATAAACCCGGCGAGAATCAAGCAATGTAGTTGGATAAGGAAGTGGGCCATTAAAAGCCATTTGGAGAGACTGAGGAAAGAGAATTCGGAGAAGGGATTCGTGGAGGTTATGAGCAATTGCCGGCTGTCGGTATGTAGCATTCTGATCTGTATATGCTTTGGAGCGAAAATCCCGGAAGAGGAGATTAAGGTTATAGAGAGTATACTGAAGGACGTGATGTTGATTACGTTGCCGAAGCTCCCTGACTTTTTGCCGGTGCTCACACCTTTGTTCCGGCGGCAACTGAAGCAAGCGAAGGAGCTGAGGAGAAAACAGTTGGAATGTCTGATACCGTTGATAAGGAGGAGAAGGTTGTTCGTGGAGAGGAATGGGGATGAGAGCGTGATGAAGGATTTGCCGGAGATGGTTAGTCCAATTGGAGCTGCGTATCTTGATTCGCTATTTGAACTGGAAACGCCTGGACGTGGGCGGTTGGGGGAGGAGGAGCTGGTCACGCTTTGTTCGGAGGTAATTAATGCCGGAACTGACACCAGCGCCACCGCGTTGGAGTGGGCTTTGCTTCATTTGGTTCAAGACCAACAAATTCAGGAAAGACTATACGACGAAATCATTAGCGTTGTGGGTAATGTTCATCCATCCCTCCTATTTTATTCAATTTAATAGTTTTAAAATAAGGTTTAAATAGATAAAAATAGCTTGTAATCATAACATTTTTAGGAACAAAATTGGAATTTATTGTGCTAAATTAAACTTAAAAGTTAACCTTTTAGTGGAATGTTGTAACAGGTAAAGATGGAGTGGTAACCGAAGGCGACGTAGAGAAAATGCCATATCTAGGGGCAGTGGTGAAAGAGACATTCCGGCGACACCCTCCCAGCCATTTCTTACTATCTCACGCGGCGACGAAAGAGACGGAGCTCGGCGGGTACACGATCCCGGCGGACGCCAGCGTGGAGTTTTACACGGCTCATTTATCGGACGACCCCAACACGTGGGAAGAACCGGGTTCGTTTCGGCCGGATAGGTTCTTGGAGGGCGACGGTGTTGGCGTGGACGTAACAGGAACTAAGGGCGTCAAGATGGTGCCGTTTGGGGCAGGGAGGCGGATCTGCCCGGCCATGACTTTGGGGACGTTGCATGTTCACATGATGCTGGCAAGAATGGTGCAGGCATTCAAGTGGGTCCCGGCTCCTGGAGCCCCCCCCGACCCGACCGAGACTTTTGCGTTTACAGTGATTATGAAGAACCCTCTGAAGGCTACCATATTGGAGAGGTCGGGT

mRNA sequence

GACGTCGCCATTTATTTTCAACTTTCAATTATCAAACACATTTCTTGATCTCGCCTCGCCTCTGCTATGGACTTACTCGATGCCGTTGTTCTTCTCCTGGCCTTGCTTTTTTTCTCCTTATGGTGGCGTTATTGTTATTCTCCAACGTCGTCCGTTTATTTTCGTGGCTCGCGATTTACGTGAAAAATATGGTCCCATTTTCACAATGCAAATGGGGCAACGCACACTCATCATTGTGTCTAGTGCGGAGTTGATTCACGAAGCTCTCGTGCAGCGTGGTCCCTTATTTGCCAGCCGTCCTGCTGACTCACCTATTCGTCTAGTCTTCAGCGTTGGAAAATGCGCTGTCAACTCAGCCGAGTATGGTCCCCTATGGCGAACACTCCGCCGTAACTTCGTGACGGAGCTGATAAACCCGGCGAGAATCAAGCAATGTAGTTGGATAAGGAAGTGGGCCATTAAAAGCCATTTGGAGAGACTGAGGAAAGAGAATTCGGAGAAGGGATTCGTGGAGGTTATGAGCAATTGCCGGCTGTCGGTATGTAGCATTCTGATCTGTATATGCTTTGGAGCGAAAATCCCGGAAGAGGAGATTAAGGTTATAGAGAGTATACTGAAGGACGTGATGTTGATTACGTTGCCGAAGCTCCCTGACTTTTTGCCGGTGCTCACACCTTTGTTCCGGCGGCAACTGAAGCAAGCGAAGGAGCTGAGGAGAAAACAGTTGGAATGTCTGATACCGTTGATAAGGAGGAGAAGGTTGTTCGTGGAGAGGAATGGGGATGAGAGCGTGATGAAGGATTTGCCGGAGATGGTTAGTCCAATTGGAGCTGCGTATCTTGATTCGCTATTTGAACTGGAAACGCCTGGACGTGGGCGGTTGGGGGAGGAGGAGCTGGTCACGCTTTGTTCGGAGGTAATTAATGCCGGAACTGACACCAGCGCCACCGCGTTGGAGTGGGCTTTGCTTCATTTGGTTCAAGACCAACAAATTCAGGAAAGACTATACGACGAAATCATTAGCGTTGTGGCCCCCCCCGACCCGACCGAGACTTTTGCGTTTACAGTGATTATGAAGAACCCTCTGAAGGCTACCATATTGGAGAGGTCGGGT

Coding sequence (CDS)

ATGCCGTTGTTCTTCTCCTGGCCTTGCTTTTTTTCTCCTTATGGTGGCGTTATTGTTATTCTCCAACGTCGTCCGTTTATTTTCGTGGCTCGCGATTTACGTGAAAAATATGGTCCCATTTTCACAATGCAAATGGGGCAACGCACACTCATCATTGTGTCTAGTGCGGAGTTGATTCACGAAGCTCTCGTGCAGCGTGGTCCCTTATTTGCCAGCCGTCCTGCTGACTCACCTATTCGTCTAGTCTTCAGCGTTGGAAAATGCGCTGTCAACTCAGCCGAGTATGGTCCCCTATGGCGAACACTCCGCCGTAACTTCGTGACGGAGCTGATAAACCCGGCGAGAATCAAGCAATGTAGTTGGATAAGGAAGTGGGCCATTAAAAGCCATTTGGAGAGACTGAGGAAAGAGAATTCGGAGAAGGGATTCGTGGAGGTTATGAGCAATTGCCGGCTGTCGGTATGTAGCATTCTGATCTGTATATGCTTTGGAGCGAAAATCCCGGAAGAGGAGATTAAGGTTATAGAGAGTATACTGAAGGACGTGATGTTGATTACGTTGCCGAAGCTCCCTGACTTTTTGCCGGTGCTCACACCTTTGTTCCGGCGGCAACTGAAGCAAGCGAAGGAGCTGAGGAGAAAACAGTTGGAATGTCTGATACCGTTGATAAGGAGGAGAAGGTTGTTCGTGGAGAGGAATGGGGATGAGAGCGTGATGAAGGATTTGCCGGAGATGGTTAGTCCAATTGGAGCTGCGTATCTTGATTCGCTATTTGAACTGGAAACGCCTGGACGTGGGCGGTTGGGGGAGGAGGAGCTGGTCACGCTTTGTTCGGAGGTAATTAATGCCGGAACTGACACCAGCGCCACCGCGTTGGAGTGGGCTTTGCTTCATTTGGTTCAAGACCAACAAATTCAGGAAAGACTATACGACGAAATCATTAGCGTTGTGGCCCCCCCCGACCCGACCGAGACTTTTGCGTTTACAGTGATTATGAAGAACCCTCTGAAGGCTACCATATTGGAGAGGTCGGGT

Protein sequence

MPLFFSWPCFFSPYGGVIVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVVAPPDPTETFAFTVIMKNPLKATILERSG

Homology

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy Swiss-Prot
Match: Q9LZ31 (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 130/296 (43.92%), Postives = 197/296 (66.55%), Query Frame = 0


Query: 24  RPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVF 83
           +PF   A DL++ YGPIFT++MG RT+II+S A L+HEAL+QRG LFASRPA++P R +F
Sbjct: 62  KPFFEYAEDLKKTYGPIFTLRMGTRTMIILSDATLVHEALIQRGALFASRPAENPTRTIF 121

Query: 84  SVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKE-NSEKG 143
           S  K  VN+A+YGP+WR+LRRN V  +++  R+K+   +R+ A+   +ER++ E     G
Sbjct: 122 SCNKFTVNAAKYGPVWRSLRRNMVQNMLSSTRLKEFGKLRQSAMDKLIERIKSEARDNDG 181

Query: 144 FVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFR 203
            + V+ N R +   IL+ +CFG ++ EE I+ ++ ILK V++   P++ D+LP+L P F 
Sbjct: 182 LIWVLKNARFAAFCILLEMCFGIEMDEETIEKMDEILKTVLMTVDPRIDDYLPILAPFFS 241

Query: 204 RQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELET 263
           ++ K+A E+RR+Q++ ++ +I RRR  ++  G +               +YLD+LF+L+ 
Sbjct: 242 KERKRALEVRREQVDYVVGVIERRRRAIQNPGSDKTASSF---------SYLDTLFDLKI 301

Query: 264 PGRGRL-GEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVV 318
            GR      EELVTLCSE +N GTDT+ TA+EW +  L+ + +IQ RLYDEI S V
Sbjct: 302 EGRKTTPSNEELVTLCSEFLNGGTDTTGTAIEWGIAQLIANPEIQSRLYDEIKSTV 348

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy Swiss-Prot
Match: P37124 (Cytochrome P450 77A2 OS=Solanum melongena OX=4111 GN=CYP77A2 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-65
Identity = 126/297 (42.42%), Postives = 203/297 (68.35%), Query Frame = 0


Query: 24  RPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVF 83
           +PF  + R+LR+KYGPIFT++MG RT+II+S+A+L+HEAL+ +G +FA+RP ++P R VF
Sbjct: 63  KPFFQIMRELRQKYGPIFTLRMGTRTMIILSNADLVHEALILKGQVFATRPRENPTRTVF 122

Query: 84  SVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKE-NSEKG 143
           S  K  VN+A YGP+WR+LR+N V   ++  R+K+   +RK A+   +E++R E ++ +G
Sbjct: 123 SCDKFTVNAAVYGPVWRSLRKNMVQNGLSSIRLKEFRAVRKSAMDKMIEKIRAEADANEG 182

Query: 144 FVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFR 203
            V V+ N R +V  IL+ +CFG ++ E+ I+ I+ ++K V++   P+L D+LP+L+P F 
Sbjct: 183 VVWVLKNARFAVFCILLAMCFGVEMDEKTIEKIDQMMKSVLIALDPRLDDYLPILSPFFS 242

Query: 204 RQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGA-AYLDSLFELE 263
           +Q K A ++R++Q++ ++P I +R+  +E           PE+     + +YLD+LF+L+
Sbjct: 243 KQRKHAMDVRKQQIKTIVPFIEQRKKILES----------PEIDKTAASFSYLDTLFDLK 302

Query: 264 TPGRGRLGE-EELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVV 318
             GR       ELVTLCSE +N GTDT+ATA+EWA+  L+++  IQ +LY+EI   V
Sbjct: 303 IEGRNSTPTYPELVTLCSEFLNGGTDTTATAIEWAIGRLIENPNIQSQLYEEIKKTV 349

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.7e-63
Identity = 132/301 (43.85%), Postives = 201/301 (66.78%), Query Frame = 0


Query: 24  RPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVF 83
           + F    RDL+ KYG IFT++MG RT+IIV+SAEL HEAL+Q+G +FASRP ++P R +F
Sbjct: 48  KQFFEYIRDLKPKYGSIFTLKMGSRTMIIVASAELAHEALIQKGQIFASRPRENPTRTIF 107

Query: 84  SVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLR---KENSE 143
           S  K +VN+A YGP+WR+LRRN V  +++P+R+K+    R+ A+   +ER+R   KEN++
Sbjct: 108 SCNKFSVNAAVYGPVWRSLRRNMVQNMLSPSRLKEFREFREIAMDKLIERIRVDAKENND 167

Query: 144 KGFVEVMSNCRLSVCSILICICFGAKIPEEE-IKVIESILKDVMLITLPKLPDFLPVLTP 203
              V  + N R +V  IL+ +CFG ++  EE I+ ++ ++KDV+++  P++ DFLP+L  
Sbjct: 168 --VVWALKNARFAVFYILVAMCFGVEMDNEEMIERVDQMMKDVLIVLDPRIDDFLPILR- 227

Query: 204 LF--RRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSL 263
           LF   +Q K+  E+R++Q+E L+PLI +RR  V+  G +               +YLD+L
Sbjct: 228 LFVGYKQRKRVNEVRKRQIETLVPLIEKRRSVVQNPGSDKTAASF---------SYLDTL 287

Query: 264 FELETPGR-GRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISV 318
           F+++  GR       ELVTLCSE +N GTDT+ATALEW +  L+++  IQ +LY EI ++
Sbjct: 288 FDVKVEGRKSGPTNAELVTLCSEFLNGGTDTTATALEWGIGRLMENPTIQNQLYQEIKTI 336

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 116/292 (39.73%), Postives = 192/292 (65.75%), Query Frame = 0


Query: 24  RPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVF 83
           +PF     D+R KYG IFT++MG RT+II++ A+L+HEA++Q+G  +A+RP ++P R +F
Sbjct: 58  KPFFEYVNDVRLKYGSIFTLKMGTRTMIILTDAKLVHEAMIQKGATYATRPPENPTRTIF 117

Query: 84  SVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKE-NSEKG 143
           S  K  VN+A YGP+W++LRRN V  +++  R+K+   +R  A+   + RL+ E     G
Sbjct: 118 SENKFTVNAATYGPVWKSLRRNMVQNMLSSTRLKEFRSVRDNAMDKLINRLKDEAEKNNG 177

Query: 144 FVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFR 203
            V V+ + R +V  IL+ +CFG ++ EE ++ I+ ++K V++   P++ D+LP+L+P F 
Sbjct: 178 VVWVLKDARFAVFCILVAMCFGLEMDEETVERIDQVMKSVLITLDPRIDDYLPILSPFFS 237

Query: 204 RQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELET 263
           +Q K+A E+RR+Q+E L+P+I +RR  ++  G +               +YLD+LF+L+ 
Sbjct: 238 KQRKKALEVRREQVEFLVPIIEQRRRAIQNPGSDHTATTF---------SYLDTLFDLKV 297

Query: 264 PG-RGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEI 314
            G +    + ELV+LCSE +N GTDT+ATA+EW +  L+ +  +Q +LY+EI
Sbjct: 298 EGKKSAPSDAELVSLCSEFLNGGTDTTATAVEWGIAQLIANPNVQTKLYEEI 340

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 182.6 bits (462), Expect = 7.7e-45
Identity = 116/316 (36.71%), Postives = 185/316 (58.54%), Query Frame = 0


Query: 31  RDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVFSVGKCAV 90
           R +  + GPI T+++  R  I V+   L HEALV  G ++A RP  + I  +  V +  +
Sbjct: 59  RSVHHRLGPIVTLRITSRPAIFVADRSLTHEALVLNGAVYADRPPPAVISKI--VDEHNI 118

Query: 91  NSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKENSEKGFVEVMSNC 150
           +S  YG  WR LRRN  +E+++P+R++  S  R W ++   ER R    E+  V ++ + 
Sbjct: 119 SSGSYGATWRLLRRNITSEILHPSRVRSYSHARHWVLEILFERFRNHGGEEPIV-LIHHL 178

Query: 151 RLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDF--LPVLTPL-FRRQLKQ 210
             ++ ++L+ +CFG K+ E++IK +E I + + L++L K   F   P  T L  R++ ++
Sbjct: 179 HYAMFALLVLMCFGDKLDEKQIKEVEFIQR-LQLLSLTKFNIFNIWPKFTKLILRKRWQE 238

Query: 211 AKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELETPGRGR 270
             ++RR+Q + L+PLIR RR  VE     S  +D  + V     +Y+D+L +LE P   R
Sbjct: 239 FLQIRRQQRDVLLPLIRARRKIVEER-KRSEQEDKKDYVQ----SYVDTLLDLELPEENR 298

Query: 271 -LGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVVAPPDPTETF 330
            L EE+++ LCSE + AGTDT+ATAL+W + +LV+  +IQERL++EI SVV   +  E  
Sbjct: 299 KLNEEDIMNLCSEFLTAGTDTTATALQWIMANLVKYPEIQERLHEEIKSVVG-EEAKEVE 358

Query: 331 AFTVIMKNPLKATILE 343
              V     LKA +LE
Sbjct: 359 EEDVEKMPYLKAVVLE 364

[Close]

BLAST of Cp4.1LG10g09700 vs. NCBI nr
Match: XP_023545118.1 (cytochrome P450 77A1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 586 bits (1511), Expect = 8.91e-207
Identity = 303/313 (96.81%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML
Sbjct: 159 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE
Sbjct: 219 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 338

Query: 306 IQERLYDEIISVV 317
           IQERLYDEIISVV
Sbjct: 339 IQERLYDEIISVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. NCBI nr
Match: KAG6603767.1 (Cytochrome P450 77A1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033936.1 Cytochrome P450 77A1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584 bits (1506), Expect = 5.13e-206
Identity = 302/313 (96.49%), Postives = 304/313 (97.12%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML
Sbjct: 159 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE
Sbjct: 219 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 338

Query: 306 IQERLYDEIISVV 317
           IQERLY+EIISVV
Sbjct: 339 IQERLYNEIISVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. NCBI nr
Match: XP_022950189.1 (cytochrome P450 77A1 [Cucurbita moschata])

HSP 1 Score: 582 bits (1501), Expect = 2.95e-205
Identity = 301/313 (96.17%), Postives = 303/313 (96.81%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML
Sbjct: 159 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE
Sbjct: 219 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQH 338

Query: 306 IQERLYDEIISVV 317
           IQERLY+EIISVV
Sbjct: 339 IQERLYNEIISVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. NCBI nr
Match: XP_022977848.1 (cytochrome P450 77A1 [Cucurbita maxima])

HSP 1 Score: 582 bits (1501), Expect = 2.95e-205
Identity = 301/313 (96.17%), Postives = 303/313 (96.81%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML
Sbjct: 159 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE
Sbjct: 219 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQH 338

Query: 306 IQERLYDEIISVV 317
           IQERLY+EIISVV
Sbjct: 339 IQERLYNEIISVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. NCBI nr
Match: XP_008439972.1 (PREDICTED: cytochrome P450 77A1 [Cucumis melo] >TYK13058.1 cytochrome P450 77A1 [Cucumis melo var. makuwa])

HSP 1 Score: 569 bits (1467), Expect = 4.51e-200
Identity = 290/313 (92.65%), Postives = 302/313 (96.49%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAI+SHLERLRKENSEKGFVEVM+NCRLSVCSILICICFGAKIPE+EIKVIESILKDVML
Sbjct: 159 WAIESHLERLRKENSEKGFVEVMTNCRLSVCSILICICFGAKIPEQEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLP+LTPLFRRQLKQAKELRRKQLECLIPLIR+RR+FVERNGDESV ++LPE
Sbjct: 219 ITLPKLPDFLPILTPLFRRQLKQAKELRRKQLECLIPLIRKRRMFVERNGDESVREELPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQD 338

Query: 306 IQERLYDEIISVV 317
           +QERLY+EII+VV
Sbjct: 339 VQERLYNEIINVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy TrEMBL
Match: A0A6J1IRA3 (cytochrome P450 77A1 OS=Cucurbita maxima OX=3661 GN=LOC111478022 PE=3 SV=1)

HSP 1 Score: 582 bits (1501), Expect = 1.43e-205
Identity = 301/313 (96.17%), Postives = 303/313 (96.81%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML
Sbjct: 159 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE
Sbjct: 219 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQH 338

Query: 306 IQERLYDEIISVV 317
           IQERLY+EIISVV
Sbjct: 339 IQERLYNEIISVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy TrEMBL
Match: A0A6J1GF34 (cytochrome P450 77A1 OS=Cucurbita moschata OX=3662 GN=LOC111453356 PE=3 SV=1)

HSP 1 Score: 582 bits (1501), Expect = 1.43e-205
Identity = 301/313 (96.17%), Postives = 303/313 (96.81%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML
Sbjct: 159 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE
Sbjct: 219 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQH 338

Query: 306 IQERLYDEIISVV 317
           IQERLY+EIISVV
Sbjct: 339 IQERLYNEIISVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy TrEMBL
Match: A0A5D3CPF5 (Cytochrome P450 77A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006600 PE=3 SV=1)

HSP 1 Score: 569 bits (1467), Expect = 2.18e-200
Identity = 290/313 (92.65%), Postives = 302/313 (96.49%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAI+SHLERLRKENSEKGFVEVM+NCRLSVCSILICICFGAKIPE+EIKVIESILKDVML
Sbjct: 159 WAIESHLERLRKENSEKGFVEVMTNCRLSVCSILICICFGAKIPEQEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLP+LTPLFRRQLKQAKELRRKQLECLIPLIR+RR+FVERNGDESV ++LPE
Sbjct: 219 ITLPKLPDFLPILTPLFRRQLKQAKELRRKQLECLIPLIRKRRMFVERNGDESVREELPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQD 338

Query: 306 IQERLYDEIISVV 317
           +QERLY+EII+VV
Sbjct: 339 VQERLYNEIINVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy TrEMBL
Match: A0A1S3B009 (cytochrome P450 77A1 OS=Cucumis melo OX=3656 GN=LOC103484599 PE=3 SV=1)

HSP 1 Score: 569 bits (1467), Expect = 2.18e-200
Identity = 290/313 (92.65%), Postives = 302/313 (96.49%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAI+SHLERLRKENSEKGFVEVM+NCRLSVCSILICICFGAKIPE+EIKVIESILKDVML
Sbjct: 159 WAIESHLERLRKENSEKGFVEVMTNCRLSVCSILICICFGAKIPEQEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLP+LTPLFRRQLKQAKELRRKQLECLIPLIR+RR+FVERNGDESV ++LPE
Sbjct: 219 ITLPKLPDFLPILTPLFRRQLKQAKELRRKQLECLIPLIRKRRMFVERNGDESVREELPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQD 338

Query: 306 IQERLYDEIISVV 317
           +QERLY+EII+VV
Sbjct: 339 VQERLYNEIINVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. ExPASy TrEMBL
Match: A0A0A0KJX8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G514850 PE=3 SV=1)

HSP 1 Score: 566 bits (1460), Expect = 2.53e-199
Identity = 289/313 (92.33%), Postives = 300/313 (95.85%), Query Frame = 0


Query: 6   SWPCFFSPYGGVI-VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 65
            WP      G +I VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV
Sbjct: 39  GWPI----VGNLIQVILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALV 98

Query: 66  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRK 125
           QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINP RIKQCSWIRK
Sbjct: 99  QRGPLFASRPADSPIRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPTRIKQCSWIRK 158

Query: 126 WAIKSHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 185
           WAI+SHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML
Sbjct: 159 WAIESHLERLRKENSEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVML 218

Query: 186 ITLPKLPDFLPVLTPLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPE 245
           ITLPKLPDFLP+LTPLF RQLKQAKELRRKQLECLIPLIR+RR+F+ERNGDESV K+LPE
Sbjct: 219 ITLPKLPDFLPILTPLFGRQLKQAKELRRKQLECLIPLIRKRRMFMERNGDESVRKELPE 278

Query: 246 MVSPIGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQ 305
           MVSP+GAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQ 
Sbjct: 279 MVSPMGAAYLDSLFELETPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQD 338

Query: 306 IQERLYDEIISVV 317
           +QERLY+EII+VV
Sbjct: 339 VQERLYNEIINVV 347

[Close]

BLAST of Cp4.1LG10g09700 vs. TAIR 10
Match: AT1G11600.1 (cytochrome P450, family 77, subfamily B, polypeptide 1 )

HSP 1 Score: 437.6 bits (1124), Expect = 9.5e-123
Identity = 212/300 (70.67%), Postives = 255/300 (85.00%), Query Frame = 0


Query: 19  VILQRRPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSP 78
           VI QRR F+F+ RDLR+KYGPIFTMQMGQRT+II++  +LIHEALVQRGP FASRP DSP
Sbjct: 51  VIFQRRHFVFLMRDLRKKYGPIFTMQMGQRTMIIITDEKLIHEALVQRGPTFASRPPDSP 110

Query: 79  IRLVFSVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKEN 138
           IRL+FSVGKCA+NSAEYG LWRTLRRNFVTEL+   R+KQCSWIR WA+++H++R++ EN
Sbjct: 111 IRLMFSVGKCAINSAEYGSLWRTLRRNFVTELVTAPRVKQCSWIRSWAMQNHMKRIKTEN 170

Query: 139 SEKGFVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLT 198
            EKGFVEVMS CRL++CSILIC+CFGAKI EE+IK IE++LKDVMLIT P LPDFLPV T
Sbjct: 171 VEKGFVEVMSQCRLTICSILICLCFGAKISEEKIKNIENVLKDVMLITSPTLPDFLPVFT 230

Query: 199 PLFRRQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLF 258
           PLFRRQ+++A+ELR+ QLECL+PLIR RR FV+   + +      EMVSPIGAAY+DSLF
Sbjct: 231 PLFRRQVREARELRKTQLECLVPLIRNRRKFVDAKENPN-----EEMVSPIGAAYVDSLF 290

Query: 259 ELETPGR-GRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVV 318
            L    R G LG+EE+VTLCSE+++AGTDTSAT LEWAL HLV DQ IQE+LY+E++ VV
Sbjct: 291 RLNLIERGGELGDEEIVTLCSEIVSAGTDTSATTLEWALFHLVTDQNIQEKLYEEVVGVV 345

HSP 2 Score: 44.3 bits (103), Expect = 2.3e-04
Identity = 19/27 (70.37%), Postives = 23/27 (85.19%), Query Frame = 0


Query: 318 APPDPTETFAFTVIMKNPLKATILERS 345
           +PPDPTET+AFTV+MKN LKA I  R+
Sbjct: 484 SPPDPTETYAFTVVMKNSLKAQIRSRT 510

[Close]

BLAST of Cp4.1LG10g09700 vs. TAIR 10
Match: AT5G04660.1 (cytochrome P450, family 77, subfamily A, polypeptide 4 )

HSP 1 Score: 260.8 bits (665), Expect = 1.6e-69
Identity = 130/296 (43.92%), Postives = 197/296 (66.55%), Query Frame = 0


Query: 24  RPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVF 83
           +PF   A DL++ YGPIFT++MG RT+II+S A L+HEAL+QRG LFASRPA++P R +F
Sbjct: 62  KPFFEYAEDLKKTYGPIFTLRMGTRTMIILSDATLVHEALIQRGALFASRPAENPTRTIF 121

Query: 84  SVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKE-NSEKG 143
           S  K  VN+A+YGP+WR+LRRN V  +++  R+K+   +R+ A+   +ER++ E     G
Sbjct: 122 SCNKFTVNAAKYGPVWRSLRRNMVQNMLSSTRLKEFGKLRQSAMDKLIERIKSEARDNDG 181

Query: 144 FVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFR 203
            + V+ N R +   IL+ +CFG ++ EE I+ ++ ILK V++   P++ D+LP+L P F 
Sbjct: 182 LIWVLKNARFAAFCILLEMCFGIEMDEETIEKMDEILKTVLMTVDPRIDDYLPILAPFFS 241

Query: 204 RQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELET 263
           ++ K+A E+RR+Q++ ++ +I RRR  ++  G +               +YLD+LF+L+ 
Sbjct: 242 KERKRALEVRREQVDYVVGVIERRRRAIQNPGSDKTASSF---------SYLDTLFDLKI 301

Query: 264 PGRGRL-GEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVV 318
            GR      EELVTLCSE +N GTDT+ TA+EW +  L+ + +IQ RLYDEI S V
Sbjct: 302 EGRKTTPSNEELVTLCSEFLNGGTDTTGTAIEWGIAQLIANPEIQSRLYDEIKSTV 348

[Close]

BLAST of Cp4.1LG10g09700 vs. TAIR 10
Match: AT5G04630.1 (cytochrome P450, family 77, subfamily A, polypeptide 9 )

HSP 1 Score: 240.4 bits (612), Expect = 2.2e-63
Identity = 124/296 (41.89%), Postives = 188/296 (63.51%), Query Frame = 0


Query: 24  RPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVF 83
           + F     ++R  YGPIFT++MG RT+II+S A L H+AL++RG  FA+RPA++P R +F
Sbjct: 59  KQFFEYVDEMRNIYGPIFTLKMGIRTMIIISDANLAHQALIERGAQFATRPAETPTRKIF 118

Query: 84  SVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKENSEK-G 143
           S     V+SA YGP+WR+LRRN V  ++   R+K+   IRK AI   +E+++ E  E  G
Sbjct: 119 SSSDITVHSAMYGPVWRSLRRNMVQNMLCSNRLKEFGSIRKSAIDKLVEKIKSEAKENDG 178

Query: 144 FVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFR 203
            V V+ N R +   IL+ +CFG K+ EE I+ ++ ++ +++    P++ D+LP+LTP + 
Sbjct: 179 LVWVLRNARFAAFCILLDMCFGVKMEEESIEKMDQMMTEILTAVDPRIHDYLPILTPFYF 238

Query: 204 RQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELET 263
           ++ K + ELRRK ++ ++  I +RRL +   G +               AYLD+LF+L  
Sbjct: 239 KERKNSLELRRKLVQFVVGFIEKRRLAIRNLGSDKTASSF---------AYLDTLFDLRV 298

Query: 264 PGRGRL-GEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVV 318
            GR     +E+LVTLCSE +NAGTDT+ TA+EW +  L+ + +IQ RLYDEI S V
Sbjct: 299 DGRETSPSDEDLVTLCSEFLNAGTDTTGTAIEWGIAELISNPKIQSRLYDEIKSTV 345

[Close]

BLAST of Cp4.1LG10g09700 vs. TAIR 10
Match: AT3G10570.1 (cytochrome P450, family 77, subfamily A, polypeptide 6 )

HSP 1 Score: 238.8 bits (608), Expect = 6.5e-63
Identity = 117/295 (39.66%), Postives = 192/295 (65.08%), Query Frame = 0


Query: 32  DLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVFSVGKCAVN 91
           D+R+KYGPI+T++MG RT+II+S + L+H+ L+QRGP+FA+RP ++P R +FS     VN
Sbjct: 72  DVRKKYGPIYTLRMGSRTMIIISDSALVHDVLIQRGPMFATRPTENPTRTIFSSNTFTVN 131

Query: 92  SAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKENSEK-GFVEVMSNC 151
           ++ YGP+WR+LR+N V  +++  R ++   +R+ A+   +ER++ E  +  G V V+ N 
Sbjct: 132 ASAYGPVWRSLRKNMVQNMLSSIRFREFGSLRQSAMDKLVERIKSEAKDNDGLVWVLRNA 191

Query: 152 RLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFRRQLKQAKE 211
           R +   IL+ +CFG ++ EE I  ++ ++K V++   P+L D+LP+L P + ++  +A E
Sbjct: 192 RFAAFCILLEMCFGIEMDEESILNMDQVMKKVLITLNPRLDDYLPILAPFYSKERARALE 251

Query: 212 LRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELETPGR-GRLG 271
           +R +Q++ ++ LI RRR  +++ G +               +YLD+LF+L+T GR     
Sbjct: 252 VRCEQVDFIVKLIERRRRAIQKPGTDKTASSF---------SYLDTLFDLKTEGRITTPS 311

Query: 272 EEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVVAPPDPTE 325
            EELV+LCSE +N GTDT+ TA+EW +  L+ + +IQ RLYDEI S V   +  E
Sbjct: 312 NEELVSLCSEFLNGGTDTTGTAIEWGIAQLIVNPEIQSRLYDEIKSTVGDREVEE 357

[Close]

BLAST of Cp4.1LG10g09700 vs. TAIR 10
Match: AT3G10560.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 215.7 bits (548), Expect = 5.9e-56
Identity = 119/323 (36.84%), Postives = 187/323 (57.89%), Query Frame = 0


Query: 24  RPFIFVARDLREKYGPIFTMQMGQRTLIIVSSAELIHEALVQRGPLFASRPADSPIRLVF 83
           + F     DL + YGPI T+++G RT+II+S A L HEAL++RG  FA+RP ++P R +F
Sbjct: 67  KQFFEYVEDLVKIYGPILTLRLGTRTMIIISDASLAHEALIERGAQFATRPVETPTRKIF 126

Query: 84  SVGKCAVNSAEYGPLWRTLRRNFVTELINPARIKQCSWIRKWAIKSHLERLRKE-NSEKG 143
           S  +  V+SA YGP+WR+LRRN V  +++  R+K+   +RK A+   +ER++ E     G
Sbjct: 127 SSSEITVHSAMYGPVWRSLRRNMVQNMLSSNRLKEFGSVRKSAMDKLIERIKSEARDNDG 186

Query: 144 FVEVMSNCRLSVCSILICICFGAKIPEEEIKVIESILKDVMLITLPKLPDFLPVLTPLFR 203
            V V+ N R +   +L+ ICFG ++ EE I+ ++ ++  ++    PKL D+LP+LTP   
Sbjct: 187 LVWVLQNSRYAAFCVLLDICFGVEMEEESIEKMDQLMTAILNAVDPKLHDYLPILTPFNY 246

Query: 204 RQLKQAKELRRKQLECLIPLIRRRRLFVERNGDESVMKDLPEMVSPIGAAYLDSLFELE- 263
            +  +A +LRR+ ++ ++  I +RR  +      S              +YLD+LF+L  
Sbjct: 247 NERNRALKLRRELVDFVVGFIEKRRKAIRTATVSSF-------------SYLDTLFDLRI 306

Query: 264 -TPGRGRLGEEELVTLCSEVINAGTDTSATALEWALLHLVQDQQIQERLYDEIISVVAPP 323
                    +E+LVTLCSE +NAGTDT+  A+EW +  L+ + +IQ RLYDEI S V   
Sbjct: 307 IEGSETTPSDEDLVTLCSEFLNAGTDTTGAAIEWGIAELIANPEIQSRLYDEIKSTVGDR 366

Query: 324 DPTETFAFTVIMKNPLKATILER 344
              E     +++   +   IL R
Sbjct: 367 AVDERDVDKMVLLQAVVKEILRR 376

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g09700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZ31	2.2e-68	43.92	Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1	[more]
P37124	3.0e-65	42.42	Cytochrome P450 77A2 OS=Solanum melongena OX=4111 GN=CYP77A2 PE=2 SV=1	[more]
P37123	3.7e-63	43.85	Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=...	[more]
O48928	1.8e-62	39.73	Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1	[more]
Q42602	7.7e-45	36.71	Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2	[more]

BLAST of Cp4.1LG10g09700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545118.1	8.91e-207	96.81	cytochrome P450 77A1 [Cucurbita pepo subsp. pepo]	[more]
KAG6603767.1	5.13e-206	96.49	Cytochrome P450 77A1, partial [Cucurbita argyrosperma subsp. sororia] >KAG703393...	[more]
XP_022950189.1	2.95e-205	96.17	cytochrome P450 77A1 [Cucurbita moschata]	[more]
XP_022977848.1	2.95e-205	96.17	cytochrome P450 77A1 [Cucurbita maxima]	[more]
XP_008439972.1	4.51e-200	92.65	PREDICTED: cytochrome P450 77A1 [Cucumis melo] >TYK13058.1 cytochrome P450 77A1 ...	[more]

BLAST of Cp4.1LG10g09700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IRA3	1.43e-205	96.17	cytochrome P450 77A1 OS=Cucurbita maxima OX=3661 GN=LOC111478022 PE=3 SV=1	[more]
A0A6J1GF34	1.43e-205	96.17	cytochrome P450 77A1 OS=Cucurbita moschata OX=3662 GN=LOC111453356 PE=3 SV=1	[more]
A0A5D3CPF5	2.18e-200	92.65	Cytochrome P450 77A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255...	[more]
A0A1S3B009	2.18e-200	92.65	cytochrome P450 77A1 OS=Cucumis melo OX=3656 GN=LOC103484599 PE=3 SV=1	[more]
A0A0A0KJX8	2.53e-199	92.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G514850 PE=3 SV=1	[more]

BLAST of Cp4.1LG10g09700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11600.1	9.5e-123	70.67	cytochrome P450, family 77, subfamily B, polypeptide 1	[more]
AT5G04660.1	1.6e-69	43.92	cytochrome P450, family 77, subfamily A, polypeptide 4	[more]
AT5G04630.1	2.2e-63	41.89	cytochrome P450, family 77, subfamily A, polypeptide 9	[more]
AT3G10570.1	6.5e-63	39.66	cytochrome P450, family 77, subfamily A, polypeptide 6	[more]
AT3G10560.1	5.9e-56	36.84	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 58..79 score: 29.53 coord: 292..318 score: 29.98 coord: 34..53 score: 31.47 coord: 272..289 score: 27.02 coord: 153..171 score: 20.44
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 8..342 e-value: 1.1E-44 score: 154.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 16..321
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 24..318 e-value: 3.7E-38 score: 131.5
None	No IPR available	PANTHER	PTHR24298:SF54	CYTOCHROME P450 LIKE PROTEIN	coord: 318..342 coord: 20..318
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 318..342 coord: 20..318

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG10g09700.1	Cp4.1LG10g09700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen