Cp4.1LG10g08270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG10g08270 vs. ExPASy Swiss-Prot
Match: Q6NY98 (mRNA-capping enzyme OS=Danio rerio OX=7955 GN=rngtt PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.3e-69
Identity = 196/594 (33.00%), Postives = 287/594 (48.32%), Query Frame = 0

Query: 83  PPGWLDCPAFGQEIC-CMIPSKVPLGDTFSDCVAPGKRYAFKQAIHQQRVWGRKLGLVID 142
           PP W +CP  GQ +    +P K  LG  + D V    R+      +  +    K+GL++D
Sbjct: 7   PPRWRNCPRRGQPVAGKFLPMKTMLGPRYDDKVPEENRFHPSMLSNYLKSLKVKMGLLVD 66

Query: 143 LTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNMFVYEVSQFISRQKHSKKYILV 202
           LTN+ R+Y   D+ KEGIKYVK+ CKG    P  ++  MF+     FI  +K   + I V
Sbjct: 67  LTNTTRFYDRADIEKEGIKYVKLSCKGHGECPTAETTEMFIRLCEHFI--EKTPTELIGV 126

Query: 203 HCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPGIYKPDYIDALYAFYHEKKPEL 262
           HCTHG NRTG++I  YL   +  S+  A+  F+ ARPPGIYK DY+  L+  Y + + + 
Sbjct: 127 HCTHGFNRTGFLICAYLVEKMDWSIEAAVAAFAQARPPGIYKGDYLKELFRRYGDVE-DA 186

Query: 263 VVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENHDD--------------AAQVM 322
              PP PEW    D + +GE      +DG ++AP +E                   A  +
Sbjct: 187 PAAPPLPEWCFDEDEEEDGE------EDGSASAPASEPSSSHTGQSKKKKERLKLGAVFL 246

Query: 323 TNDDILGDEIPEDQER--AFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQLLRQRY 382
               + G      Q +    +  C Q  + +        FPG+ PVS++R+N+++L Q  
Sbjct: 247 EGVSVKGVSQVTTQPKLGEIQRKCQQFSEWDRSG-----FPGAQPVSMDRKNIRMLEQNG 306

Query: 383 YYATWKADGTRYMMLIT-MDGCYLIDR-GFKFRRVQMRFPYRNANDGLVEKIH-NYTLLD 442
           Y  +WKADGTRYMMLI   +  Y+IDR    F    + FP+R        +IH + TLLD
Sbjct: 307 YKVSWKADGTRYMMLIDGRNEVYMIDRDNSVFHIENLEFPFRKD-----LRIHLSNTLLD 366

Query: 443 GEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNIYQ 502
           GEMIID +  + +   RYLIYD++  +   V +  F  R   +EKE+I PR +E+  + Q
Sbjct: 367 GEMIIDKV--NGQPVPRYLIYDIIKFSGQPVGQCDFNRRLLCIEKEIISPR-FEKMKLGQ 426

Query: 503 SRNPQYRYDLEPFRVRRKDFWLLSTVTKLLK-EFIPRLSHDADGLIFQMGDDDSQTLILF 562
               +     EPF VR K F+ +    KLL+  F  ++SH+ DGLIF       Q +  +
Sbjct: 427 IDKAK-----EPFSVRNKPFFDIHAARKLLEGSFTSQVSHEVDGLIF-------QPIGKY 486

Query: 563 ERG------KRKTMEGNRVKFK---------------------------------DGDPS 617
           + G      K K    N V F+                                   D  
Sbjct: 487 KPGRCDDILKWKPPSHNSVDFRLKITKVGGEGLIPQTVGLLYVGNYDMPFAQMKITKDLK 546

BLAST of Cp4.1LG10g08270 vs. ExPASy Swiss-Prot
Match: O60942 (mRNA-capping enzyme OS=Homo sapiens OX=9606 GN=RNGTT PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 6.0e-67
Identity = 188/587 (32.03%), Postives = 288/587 (49.06%), Query Frame = 0

Query: 80  SRLPPGWLDCPAFGQEIC-CMIPSKVPLGDTFSDCVAPGKRYAFKQAIHQQRVWGRKLGL 139
           +++PP WL+CP  GQ +    +P K  LG  +   VA   R+      +  +    K+GL
Sbjct: 4   NKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVKMGL 63

Query: 140 VIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNMFVYEVSQFISRQKHSKKY 199
           ++DLTN+ R+Y   D+ KEGIKY+K+ CKG    P  ++   F+    +F   +++  + 
Sbjct: 64  LVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERF--NERNPPEL 123

Query: 200 ILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPGIYKPDYIDALYAFYHEKK 259
           I VHCTHG NRTG++I  +L   +  S+  A+  F+ ARPPGIYK DY+  L+  Y + +
Sbjct: 124 IGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIE 183

Query: 260 PELVVYPPTPEWKRSSDLDLNGEAVPDDDDDG------GSAAPLNENHDD----AAQVMT 319
            E    P  P+W    D D       D+D+DG      GS+A   +   +     A  + 
Sbjct: 184 -EAPPPPLLPDWCFEDDED------EDEDEDGKKESEPGSSASFGKRRKERLKLGAIFLE 243

Query: 320 NDDILGDEIPEDQER--AFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQLLRQRYY 379
              + G      Q +    +  C+Q             FPG+ PVS++++N++LL  + Y
Sbjct: 244 GVTVKGVTQVTTQPKLGEVQQKCHQFCGWEGSG-----FPGAQPVSMDKQNIKLLDLKPY 303

Query: 380 YATWKADGTRYMMLIT-MDGCYLIDR-GFKFRRVQMRFPYRNANDGLVEKIHNYTLLDGE 439
             +WKADGTRYMMLI   +  ++IDR    F    + FP+R     L   + N TLLDGE
Sbjct: 304 KVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRK---DLRMHLSN-TLLDGE 363

Query: 440 MIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNIYQSR 499
           MIID +  + +   RYLIYD++  N   V +  F  R + +E+E+I PR+ + +     +
Sbjct: 364 MIIDRV--NGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDK 423

Query: 500 NPQYRYDLEPFRVRRKDFWLLSTVTKLLK-EFIPRLSHDADGLIFQ-------------- 559
                   EPF VR K F+ + T  KLL+  F   +SH+ DGLIFQ              
Sbjct: 424 TQ------EPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDIL 483

Query: 560 ------MGDDDSQTLILFERGKRKTMEGNRVKFKDG------------DPSFYSGKIVEC 619
                 +   D +  I    G+    +   + +  G            +   Y  KI+EC
Sbjct: 484 KWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIEC 543

BLAST of Cp4.1LG10g08270 vs. ExPASy Swiss-Prot
Match: O55236 (mRNA-capping enzyme OS=Mus musculus OX=10090 GN=Rngtt PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.3e-65
Identity = 181/581 (31.15%), Postives = 285/581 (49.05%), Query Frame = 0

Query: 80  SRLPPGWLDCPAFGQEIC-CMIPSKVPLGDTFSDCVAPGKRYAFKQAIHQQRVWGRKLGL 139
           +++PP WL+CP  GQ +    +P K  LG  +   VA   R+      +  +    K+ L
Sbjct: 4   NKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVKMSL 63

Query: 140 VIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNMFVYEVSQFISRQKHSKKY 199
           ++DLTN+ R+Y   D+ KEGIKY+K+ CKG    P  ++   F+    +F   ++   + 
Sbjct: 64  LVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERF--NERSPPEL 123

Query: 200 ILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPGIYKPDYIDALYAFYHEKK 259
           I VHCTHG NRTG++I  +L   +  S+  A+  F+ ARPPGIYK DY+  L+  Y + +
Sbjct: 124 IGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIE 183

Query: 260 PELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENHDD----AAQVMTNDDILG 319
            E    P  P+W    + + + +     D + GS+A  ++   +     A  +    + G
Sbjct: 184 -EAPPPPVLPDWCFEDEDEEDEDEDGKKDSEPGSSASFSKRRKERLKLGAIFLEGITVKG 243

Query: 320 DEIPEDQER--AFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQLLRQRYYYATWKA 379
                 Q +    +  C+Q             FPG+ PVS++++N++LL Q+ Y  +WKA
Sbjct: 244 VTQVTTQPKLGEVQQKCHQFCGWEGSG-----FPGAQPVSMDKQNIRLLEQKPYKVSWKA 303

Query: 380 DGTRYMMLIT-MDGCYLIDR-GFKFRRVQMRFPYRNANDGLVEKIHNYTLLDGEMIIDTL 439
           DGTRYMMLI   +  ++IDR    F    + FP+R     L   + N TLLDGEMIID +
Sbjct: 304 DGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRK---DLRMHLSN-TLLDGEMIIDKV 363

Query: 440 PDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNIYQSRNPQYRY 499
             + +   RYLIYD++  N   V +  F  R + +E+E+I PR+ + +     +      
Sbjct: 364 --NGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIISPRHEKMKTGLIDKTQ---- 423

Query: 500 DLEPFRVRRKDFWLLSTVTKLLK-EFIPRLSHDADGLIFQ-------------------- 559
             EPF VR K F+ ++   KLL+  F   +SH+ DGLIFQ                    
Sbjct: 424 --EPFSVRPKQFFDINISRKLLEGNFAKEVSHEMDGLIFQPIGKYKPGRCDDILKWKPPS 483

Query: 560 MGDDDSQTLILFERGKRKTMEGNRVKFKDG------------DPSFYSGKIVECSWDSDE 619
           +   D +  I    G+    +   + +  G            +   Y  KI+EC ++++ 
Sbjct: 484 LNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNS 543

BLAST of Cp4.1LG10g08270 vs. ExPASy Swiss-Prot
Match: Q17607 (mRNA-capping enzyme OS=Caenorhabditis elegans OX=6239 GN=cel-1 PE=1 SV=2)

HSP 1 Score: 232.3 bits (591), Expect = 1.6e-59
Identity = 179/601 (29.78%), Postives = 284/601 (47.25%), Query Frame = 0

Query: 79  RSRLPPGWLDCPAFGQEI-CCMIPSKVPLGDTFSDCVAPGKRYAFKQA--IHQQRVWGRK 138
           R  LP  WL CP  G  I     P K PL   + + +A  +RY F  A       + G+K
Sbjct: 12  RMGLPDRWLHCPKTGTLINNLFFPFKTPLCKMYDNQIAE-RRYQFHPAEVFSHPHLHGKK 71

Query: 139 LGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNMFVYEVSQFISRQKHS 198
           +GL IDLTN+ RYY   ++ +    Y K+   GR   P  +  + F+  V +F   +K+ 
Sbjct: 72  IGLWIDLTNTDRYYFREEVTEHECIYHKMKMAGRGVSPTQEDTDNFIKLVQEF--HKKYP 131

Query: 199 KKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPGIYKPDYIDALYAFYH 258
            + + VHCTHG NRTG++I  YL +     +  A+  F++ R  GIYK DYID L+A Y 
Sbjct: 132 DRVVGVHCTHGFNRTGFLIAAYLFQVEEYGLDAAIGEFAENRQKGIYKQDYIDDLFARYD 191

Query: 259 EKKPELVVYPPTPEWKRSSDLDLN----------GEAVPDDDDDGGSAAPLNENHDDAAQ 318
             + + ++ P  P+W+R   + ++           + +P  + +            D ++
Sbjct: 192 PTEDDKILAPEKPDWEREMSIGMSTQIDNGRPSTSQQIPATNGNNNQNGNQLSGGGDNSK 251

Query: 319 VMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQ-FPGSHPVSLNRENLQLLRQR 378
           +  +  I G ++ ED+ +          K+    + N Q FPG  PVSL+R N+ LL Q 
Sbjct: 252 LFMDGLIRGVKVCEDEGKK----SMLQAKIKNLCKYNKQGFPGLQPVSLSRGNINLLEQE 311

Query: 379 YYYATWKADGTRYMMLITMDGCYLIDRGFK-FRRVQMRFPYRNANDGLVEKIHNYTLLDG 438
            Y  +WKADG RY++ I     Y  DR  + F    + F  +N    L+E     TL+D 
Sbjct: 312 SYMVSWKADGMRYIIYINDGDVYAFDRDNEVFEIENLDFVTKNGAP-LME-----TLVDT 371

Query: 439 EMIID--TLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNIY 498
           E+IID   +  +   + R LIYD+M  N V+V + PFY+R+++++ E+I+ R        
Sbjct: 372 EVIIDKVEINGAMCDQPRMLIYDIMRFNSVNVMKEPFYKRFEIIKTEIIDMRT------A 431

Query: 499 QSRNPQYRYDLEPFRVRRKDFWLLSTVTKLL-KEFIPRLSHDADGLIFQMGDDDSQTLIL 558
             +  + +++ +   VRRKDF+ L    KL   +F+  + H+ DGLIFQ      +T   
Sbjct: 432 AFKTGRLKHENQIMSVRRKDFYDLEATAKLFGPKFVQHVGHEIDGLIFQPKKTKYETGRC 491

Query: 559 FERGKRKTMEGNRVKF--------KDG---------------DP----------SFYSGK 618
            +  K K    N V F        K+G               DP            Y  K
Sbjct: 492 DKVLKWKPPSHNSVDFLLKVEKKCKEGMLPEWIGYLFVQNLSDPFGTMKATATLKKYHNK 551

Query: 619 IVECSWDSDE----RVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIR 625
           I+EC+   D     + W  MR RTDK+ PN   T   V+ ++ + +TE  L++ ++  +R
Sbjct: 552 IIECTLLVDNQGRPKEWKFMRERTDKSLPNGLRTAENVVETMVNPVTETYLIEYVNHALR 593

BLAST of Cp4.1LG10g08270 vs. ExPASy Swiss-Prot
Match: P40997 (mRNA-capping enzyme subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ceg1 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.0e-26
Identity = 105/344 (30.52%), Postives = 157/344 (45.64%), Query Frame = 0

Query: 345 FPGSHPVSLNRENLQLLRQRYYYATWKADGTRYMMLIT-------MDGCYLIDRGFKFRR 404
           FPGS PVS ++++LQ L+++ Y+   K+DG R ++ +T           YL DR   F  
Sbjct: 41  FPGSQPVSFSKKHLQALKEKNYFVCEKSDGIRCLLYMTEHPRYENRPSVYLFDRKMNFYH 100

Query: 405 VQMRFPYRNANDGLVEKIHNYTLLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERP 464
           V+  F Y   ND   +K H  TLLDGE+++D  P  +KQ  RYL++D +A + +    R 
Sbjct: 101 VEKIF-YPVENDKSGKKYHVDTLLDGELVLDIYPGGKKQ-LRYLVFDCLACDGIVYMSRL 160

Query: 465 FYERWKMLEKEVIEPRN-YERQNIYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFI 524
             +R  +  K + +P + Y + ++ ++        + PF    K   L   + KL  E I
Sbjct: 161 LDKRLGIFAKSIQKPLDEYTKTHMRETA-------IFPFLTSLKKMELGHGILKLFNEVI 220

Query: 525 PRLSHDADGLIFQMGD-----DDSQTLILFERGKRKTMEGNRVKF---------KDGD-- 584
           PRL H  DGLIF   +        Q+L+     K K  E N + F         ++GD  
Sbjct: 221 PRLRHGNDGLIFTCTETPYVSGTDQSLL-----KWKPKEMNTIDFMLKLEFAQPEEGDID 280

Query: 585 ----PSFYSG---------------------------------KIVECSWDSDERVWVCM 628
               P F  G                                 +IVEC  D DE  W  +
Sbjct: 281 YSAMPEFQLGVWEGRNMYSFFAFMYVDEKEWEKLKSFNVPLSERIVECYLD-DENRWRFL 340

BLAST of Cp4.1LG10g08270 vs. NCBI nr
Match: XP_023543695.1 (mRNA-capping enzyme-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1304 bits (3374), Expect = 0.0
Identity = 647/677 (95.57%), Postives = 647/677 (95.57%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180
           AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM
Sbjct: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180

Query: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240
           FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG
Sbjct: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240

Query: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300
           IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH
Sbjct: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300

Query: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360
           DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL
Sbjct: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360

Query: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420
           LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL
Sbjct: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420

Query: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480
           LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI
Sbjct: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480

Query: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ----------- 540
           YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ           
Sbjct: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRTH 540

Query: 541 -------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE 600
                              MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE
Sbjct: 541 EGLLKWKYPEMNSVDFLFEMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE 600

Query: 601 CSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR 647
           CSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR
Sbjct: 601 CSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR 660

BLAST of Cp4.1LG10g08270 vs. NCBI nr
Match: XP_023543692.1 (mRNA-capping enzyme-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543694.1 mRNA-capping enzyme-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1303 bits (3373), Expect = 0.0
Identity = 647/678 (95.43%), Postives = 647/678 (95.43%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180
           AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM
Sbjct: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180

Query: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240
           FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG
Sbjct: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240

Query: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300
           IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH
Sbjct: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300

Query: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360
           DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL
Sbjct: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360

Query: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420
           LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL
Sbjct: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420

Query: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480
           LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI
Sbjct: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480

Query: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ----------- 540
           YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ           
Sbjct: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRTH 540

Query: 541 --------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600
                               MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV
Sbjct: 541 EGLLKWKYPEMNSVDFLFEVMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600

Query: 601 ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 647
           ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD
Sbjct: 601 ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 660

BLAST of Cp4.1LG10g08270 vs. NCBI nr
Match: XP_022950699.1 (mRNA-capping enzyme-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1294 bits (3349), Expect = 0.0
Identity = 645/678 (95.13%), Postives = 645/678 (95.13%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDRHEV YSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRHEVVYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQ-RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180
           AFKQAIHQQ RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN
Sbjct: 121 AFKQAIHQQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180

Query: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240
           MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP
Sbjct: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240

Query: 241 GIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300
           GIYKPDYIDALYAFYHEKKPELVV PPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN
Sbjct: 241 GIYKPDYIDALYAFYHEKKPELVVCPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300

Query: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360
           HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ
Sbjct: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360

Query: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420
           LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT
Sbjct: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420

Query: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480
           LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN
Sbjct: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480

Query: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ---------- 540
           IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ          
Sbjct: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRT 540

Query: 541 --------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600
                               MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV
Sbjct: 541 HEGLLKWKYPEMNSVDFLFEMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600

Query: 601 ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 647
           ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD
Sbjct: 601 ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 660

BLAST of Cp4.1LG10g08270 vs. NCBI nr
Match: XP_022950697.1 (mRNA-capping enzyme-like isoform X1 [Cucurbita moschata] >XP_022950698.1 mRNA-capping enzyme-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 645/679 (94.99%), Postives = 645/679 (94.99%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDRHEV YSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRHEVVYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQ-RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180
           AFKQAIHQQ RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN
Sbjct: 121 AFKQAIHQQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180

Query: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240
           MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP
Sbjct: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240

Query: 241 GIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300
           GIYKPDYIDALYAFYHEKKPELVV PPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN
Sbjct: 241 GIYKPDYIDALYAFYHEKKPELVVCPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300

Query: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360
           HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ
Sbjct: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360

Query: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420
           LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT
Sbjct: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420

Query: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480
           LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN
Sbjct: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480

Query: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ---------- 540
           IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ          
Sbjct: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRT 540

Query: 541 ---------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKI 600
                                MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKI
Sbjct: 541 HEGLLKWKYPEMNSVDFLFEVMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKI 600

Query: 601 VECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYA 647
           VECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYA
Sbjct: 601 VECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYA 660

BLAST of Cp4.1LG10g08270 vs. NCBI nr
Match: XP_022978112.1 (mRNA-capping enzyme-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 639/677 (94.39%), Postives = 642/677 (94.83%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDR+EV YSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRNEVVYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           +QQ ARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  HQQAARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180
           AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM
Sbjct: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180

Query: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240
           FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG
Sbjct: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240

Query: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300
           IYKPDYIDALY FYHEKKPELVV P TPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH
Sbjct: 241 IYKPDYIDALYDFYHEKKPELVVCPTTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300

Query: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360
           DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL
Sbjct: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360

Query: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420
           LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL
Sbjct: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420

Query: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480
           LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI
Sbjct: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480

Query: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ----------- 540
           YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ           
Sbjct: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRTH 540

Query: 541 -------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE 600
                              MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE
Sbjct: 541 EGLLKWKYPEMNSVDFLFEMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE 600

Query: 601 CSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR 647
           CSWDSDERVWVCMR+RTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR
Sbjct: 601 CSWDSDERVWVCMRVRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR 660

BLAST of Cp4.1LG10g08270 vs. ExPASy TrEMBL
Match: A0A6J1GGL8 (GTP--RNA guanylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111453716 PE=4 SV=1)

HSP 1 Score: 1294 bits (3349), Expect = 0.0
Identity = 645/678 (95.13%), Postives = 645/678 (95.13%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDRHEV YSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRHEVVYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQ-RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180
           AFKQAIHQQ RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN
Sbjct: 121 AFKQAIHQQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180

Query: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240
           MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP
Sbjct: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240

Query: 241 GIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300
           GIYKPDYIDALYAFYHEKKPELVV PPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN
Sbjct: 241 GIYKPDYIDALYAFYHEKKPELVVCPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300

Query: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360
           HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ
Sbjct: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360

Query: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420
           LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT
Sbjct: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420

Query: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480
           LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN
Sbjct: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480

Query: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ---------- 540
           IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ          
Sbjct: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRT 540

Query: 541 --------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600
                               MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV
Sbjct: 541 HEGLLKWKYPEMNSVDFLFEMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600

Query: 601 ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 647
           ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD
Sbjct: 601 ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 660

BLAST of Cp4.1LG10g08270 vs. ExPASy TrEMBL
Match: A0A6J1GFL6 (GTP--RNA guanylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111453716 PE=4 SV=1)

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 645/679 (94.99%), Postives = 645/679 (94.99%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDRHEV YSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRHEVVYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQ-RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180
           AFKQAIHQQ RVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN
Sbjct: 121 AFKQAIHQQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180

Query: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240
           MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP
Sbjct: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240

Query: 241 GIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300
           GIYKPDYIDALYAFYHEKKPELVV PPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN
Sbjct: 241 GIYKPDYIDALYAFYHEKKPELVVCPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300

Query: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360
           HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ
Sbjct: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360

Query: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420
           LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT
Sbjct: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420

Query: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480
           LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN
Sbjct: 421 LLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQN 480

Query: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ---------- 540
           IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ          
Sbjct: 481 IYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRT 540

Query: 541 ---------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKI 600
                                MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKI
Sbjct: 541 HEGLLKWKYPEMNSVDFLFEVMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKI 600

Query: 601 VECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYA 647
           VECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYA
Sbjct: 601 VECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYA 660

BLAST of Cp4.1LG10g08270 vs. ExPASy TrEMBL
Match: A0A6J1IK92 (GTP--RNA guanylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111478187 PE=4 SV=1)

HSP 1 Score: 1285 bits (3326), Expect = 0.0
Identity = 639/677 (94.39%), Postives = 642/677 (94.83%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDR+EV YSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRNEVVYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           +QQ ARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  HQQAARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180
           AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM
Sbjct: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180

Query: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240
           FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG
Sbjct: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240

Query: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300
           IYKPDYIDALY FYHEKKPELVV P TPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH
Sbjct: 241 IYKPDYIDALYDFYHEKKPELVVCPTTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300

Query: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360
           DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL
Sbjct: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360

Query: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420
           LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL
Sbjct: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420

Query: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480
           LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI
Sbjct: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480

Query: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ----------- 540
           YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ           
Sbjct: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRTH 540

Query: 541 -------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE 600
                              MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE
Sbjct: 541 EGLLKWKYPEMNSVDFLFEMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE 600

Query: 601 CSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR 647
           CSWDSDERVWVCMR+RTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR
Sbjct: 601 CSWDSDERVWVCMRVRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR 660

BLAST of Cp4.1LG10g08270 vs. ExPASy TrEMBL
Match: A0A6J1ILU7 (GTP--RNA guanylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111478187 PE=4 SV=1)

HSP 1 Score: 1285 bits (3325), Expect = 0.0
Identity = 639/678 (94.25%), Postives = 642/678 (94.69%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETFDR+EV YSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE
Sbjct: 1   MDLNASPVPEEDEETFDRNEVVYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           +QQ ARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY
Sbjct: 61  HQQAARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120

Query: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180
           AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM
Sbjct: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180

Query: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240
           FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG
Sbjct: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240

Query: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300
           IYKPDYIDALY FYHEKKPELVV P TPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH
Sbjct: 241 IYKPDYIDALYDFYHEKKPELVVCPTTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300

Query: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360
           DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL
Sbjct: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360

Query: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420
           LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL
Sbjct: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420

Query: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480
           LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI
Sbjct: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480

Query: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ----------- 540
           YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ           
Sbjct: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRTH 540

Query: 541 --------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600
                               MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV
Sbjct: 541 EGLLKWKYPEMNSVDFLFEVMGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIV 600

Query: 601 ECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 647
           ECSWDSDERVWVCMR+RTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD
Sbjct: 601 ECSWDSDERVWVCMRVRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYAD 660

BLAST of Cp4.1LG10g08270 vs. ExPASy TrEMBL
Match: A0A6J1EUX3 (GTP--RNA guanylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111438145 PE=4 SV=1)

HSP 1 Score: 1228 bits (3176), Expect = 0.0
Identity = 603/677 (89.07%), Postives = 627/677 (92.61%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASPVPEEDEETF+RHE  YSAPE+HIE+GASIARREREERKRRLKRDRSIERPVHE
Sbjct: 5   MDLNASPVPEEDEETFERHEEVYSAPEEHIESGASIARREREERKRRLKRDRSIERPVHE 64

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
           YQQPARDQ YPAKNQK  R+RLPPGWLDCPAFGQEICCMIPSKVPLG++F+DC+ PGKRY
Sbjct: 65  YQQPARDQFYPAKNQKYDRNRLPPGWLDCPAFGQEICCMIPSKVPLGESFNDCITPGKRY 124

Query: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180
           +FKQ IHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM
Sbjct: 125 SFKQVIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 184

Query: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPPG 240
           FVYEV QF+SRQKHSKKYILVHCTHGHNRTGYMIINYL RALSISVTQALK FSDARPPG
Sbjct: 185 FVYEVIQFLSRQKHSKKYILVHCTHGHNRTGYMIINYLVRALSISVTQALKTFSDARPPG 244

Query: 241 IYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 300
           IYKPDYIDALYAFYHEKKPE+VV PPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH
Sbjct: 245 IYKPDYIDALYAFYHEKKPEMVVCPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNENH 304

Query: 301 DDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQL 360
           DD  Q+MTNDDILGDEIPEDQERA KHFCYQMLK+NAGARANLQFPGSHPVSLNR+NLQL
Sbjct: 305 DDGTQMMTNDDILGDEIPEDQERALKHFCYQMLKLNAGARANLQFPGSHPVSLNRDNLQL 364

Query: 361 LRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYTL 420
           LRQRYYYATWKADGTRYMMLIT+DGCYLIDRGFKFRRVQMRFP RNANDGLVEK H++TL
Sbjct: 365 LRQRYYYATWKADGTRYMMLITVDGCYLIDRGFKFRRVQMRFPCRNANDGLVEKSHHFTL 424

Query: 421 LDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQNI 480
           LDGEMIIDT+PDSQKQERRYLIYDMMAINHVSV ERPFYERWKMLEKEVIEPRNYERQNI
Sbjct: 425 LDGEMIIDTVPDSQKQERRYLIYDMMAINHVSVIERPFYERWKMLEKEVIEPRNYERQNI 484

Query: 481 YQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ----------- 540
           YQSRN  YRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ           
Sbjct: 485 YQSRNLHYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQGWDDAYVPRTH 544

Query: 541 -------------------MGDDDSQTLILFERGKRKTMEGNRVKFKDGDPSFYSGKIVE 600
                              +G+DDSQ LILFERGKRKTM+GNRVKFKDGDPSFYSGKIVE
Sbjct: 545 EGLLKWKYPEMNSVDFLFELGEDDSQILILFERGKRKTMDGNRVKFKDGDPSFYSGKIVE 604

Query: 601 CSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPMYADR 647
           CSWDSDE+VWVCMRIRTDKTTPNDFNTY+KVMRSI+DNITEEDLLKEIHEIIRLPMYADR
Sbjct: 605 CSWDSDEQVWVCMRIRTDKTTPNDFNTYKKVMRSIRDNITEEDLLKEIHEIIRLPMYADR 664

BLAST of Cp4.1LG10g08270 vs. TAIR 10
Match: AT3G09100.2 (mRNA capping enzyme family protein )

HSP 1 Score: 876.7 bits (2264), Expect = 1.1e-254
Identity = 443/675 (65.63%), Postives = 524/675 (77.63%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASP PEED+E + RH   YS+ ++ IE+   IARREREERK+R++ D    +P H 
Sbjct: 5   MDLNASPQPEEDDEPYVRHLEDYSSRDERIESAVEIARREREERKKRMRYD----KPTHN 64

Query: 61  YQQPARDQSYPAKNQKTV-RSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKR 120
            Q   RDQ Y  +N K   R ++P GWLDCP  G EI  ++PSKVPL +++++ V PG R
Sbjct: 65  SQPVFRDQYYQNRNTKAYDRYKIPQGWLDCPPSGNEIGFLVPSKVPLNESYNNHVPPGSR 124

Query: 121 YAFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180
           Y+FKQ IH QR+ GRKLGLVIDLTN+ RYY+TTDL KEGIK+VKI CKGRD+VPDN SVN
Sbjct: 125 YSFKQVIHNQRIAGRKLGLVIDLTNTTRYYSTTDLKKEGIKHVKIACKGRDAVPDNVSVN 184

Query: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240
            FV EV+QF+   KHSKKYILVHCTHGHNRTG+MI++YL R+  ++VTQALK+FSDARPP
Sbjct: 185 AFVNEVNQFVLNLKHSKKYILVHCTHGHNRTGFMIVHYLMRSGPMNVTQALKIFSDARPP 244

Query: 241 GIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDD-DDDGGSAAPLNE 300
           GIYKPDYIDALY+FYHE KPE V+ P TPEWKRS++LDLNGEA+PDD DDDGG A P+  
Sbjct: 245 GIYKPDYIDALYSFYHEIKPESVICPSTPEWKRSTELDLNGEALPDDEDDDGGPAGPVQG 304

Query: 301 NHDDAAQV---MTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNR 360
             +++ QV   M+NDD+LGDEIP DQE  ++ F Y+ML +N G R   QFPGSHPVSLNR
Sbjct: 305 FQEESHQVDVKMSNDDVLGDEIPPDQEEGYRQFFYRMLSLNIGGRGCSQFPGSHPVSLNR 364

Query: 361 ENLQLLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKI 420
           ENLQLLRQRYYYATWKADGTRYMML+T DGCY++DR F+FRRVQMRFP+R+  +G+ +K+
Sbjct: 365 ENLQLLRQRYYYATWKADGTRYMMLLTTDGCYIVDRSFRFRRVQMRFPFRHPTEGISDKV 424

Query: 421 HNYTLLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNY 480
           H++TLLDGEMIIDTLPD QKQERRYLIYDM+AIN  SV ERPFYERWKMLEKEVI+PRN+
Sbjct: 425 HHFTLLDGEMIIDTLPDKQKQERRYLIYDMVAINGQSVVERPFYERWKMLEKEVIDPRNH 484

Query: 481 ERQNIYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQMGDD-- 540
           E+     +R+  YRYDLEPFRVRRKDFWLLS V K+LK FIP LSH+ADGLIFQ  DD  
Sbjct: 485 EK-----ARSHIYRYDLEPFRVRRKDFWLLSAVEKVLKGFIPSLSHEADGLIFQGWDDPY 544

Query: 541 ----------------------------DSQTLILFERGKRKTMEGNRVKFK-DGDPSFY 600
                                           L LFERGK+K M+GN V F+ D DP+ Y
Sbjct: 545 VPRTHEGLLKWKYPEMNSVDFLYEQDESGRGMLSLFERGKKKHMDGNSVVFRDDSDPAEY 604

Query: 601 SGKIVECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRL 640
           SGKIVECSWD DE+VWV MR+R DK+TPND NT+RKVMRSIKDNITEE LL+EI EIIRL
Sbjct: 605 SGKIVECSWDQDEKVWVSMRVRVDKSTPNDINTFRKVMRSIKDNITEEVLLQEIREIIRL 664

BLAST of Cp4.1LG10g08270 vs. TAIR 10
Match: AT5G01290.1 (mRNA capping enzyme family protein )

HSP 1 Score: 801.2 bits (2068), Expect = 6.1e-232
Identity = 417/673 (61.96%), Postives = 499/673 (74.15%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASP PEED+E F R        ED +E+   IARREREERK+R++ D    RP   
Sbjct: 1   MDLNASPQPEEDDEPFKRRH------EDRMESAVEIARREREERKKRMRFD----RPTRV 60

Query: 61  YQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKRY 120
            Q   RDQ    +     +S+LP GWLDCP FG EI C+IPSKVPL +++++ V PGKRY
Sbjct: 61  SQPAFRDQYRDTRVYD--QSKLPQGWLDCPGFGLEIGCIIPSKVPLSESYNEHVPPGKRY 120

Query: 121 AFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVNM 180
           +FKQ +  QR+ GRKLGLVIDLTN+ RYY T DL K+GIK+VKI C+GRD+VPDN SVN 
Sbjct: 121 SFKQVVRNQRINGRKLGLVIDLTNTTRYYPTLDLKKDGIKHVKIACRGRDAVPDNVSVNT 180

Query: 181 FVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARAL-SISVTQALKMFSDARPP 240
           FV EV QF+  QKH+KKY+LVHCTHGHNRTG+MI++YL R++ +++VTQALK+FSDARPP
Sbjct: 181 FVNEVLQFVLNQKHAKKYVLVHCTHGHNRTGFMIVHYLMRSMPTMNVTQALKLFSDARPP 240

Query: 241 GIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDDDDDGGSAAPLNEN 300
           GIYKPDYIDALY FYHE KPE V  PPTPEWKRS++LDLNGEAV DDDDD     P+ E 
Sbjct: 241 GIYKPDYIDALYTFYHEIKPESVTCPPTPEWKRSAELDLNGEAVQDDDDDDSPPDPVQEI 300

Query: 301 HDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNRENLQ 360
           + +  + M+NDD LGDEIP  QE A++ FCY+ML MN G R  +QFPGSHPVSL+RE+LQ
Sbjct: 301 NQENVK-MSNDDTLGDEIPHYQEEAYRQFCYKMLMMNVGGRGFMQFPGSHPVSLDRESLQ 360

Query: 361 LLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKIHNYT 420
           LLRQRYYYATWKADGTRYMML+T+DGCYLIDR FKFRRVQMRFP +++ +G+ +K+H+YT
Sbjct: 361 LLRQRYYYATWKADGTRYMMLLTIDGCYLIDRSFKFRRVQMRFPCKHSREGISDKVHHYT 420

Query: 421 LLDGEMIIDTLPDSQ-KQERRYLIYDMMAINHVSVTERPFYERWKMLEKEVIEPRNYERQ 480
           LLDGEM+IDT    Q +  RRYL+YDM+AIN  SV ER F ERW M  +EVI PR  E+ 
Sbjct: 421 LLDGEMVIDTPTGEQGEARRRYLVYDMVAINGESVVERTFCERWNMFVREVIGPRAAEK- 480

Query: 481 NIYQSRNPQYRYDLEPFRVRRKDFWLLSTVTKLLKEFIPRLSHDADGLIFQ--------- 540
                R+  YRYDLEPF VR K FWLLSTV KLLK  IP LSH+ADGLIFQ         
Sbjct: 481 ----LRSHCYRYDLEPFAVRMKGFWLLSTVEKLLKNTIPSLSHEADGLIFQGWDDPYVPR 540

Query: 541 ---------------------MGDDDSQ-TLILFERGKRKTMEGNRVKFK-DGDPSFYSG 600
                                MG+++ +  L L ERGK+K MEG  V+F+ D DPS Y+G
Sbjct: 541 THKGLLKWKYAEMNSVDFLYEMGEEEGRGFLFLHERGKKKLMEGYSVEFRDDSDPSSYNG 600

Query: 601 KIVECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKDNITEEDLLKEIHEIIRLPM 640
           KIVEC+WD D++VW  MRIR DKTTPND NT RKV++SI DNITEE LL+EI EIIRLPM
Sbjct: 601 KIVECAWDKDKKVWFSMRIRVDKTTPNDINTARKVIKSINDNITEEVLLQEIREIIRLPM 655

BLAST of Cp4.1LG10g08270 vs. TAIR 10
Match: AT3G09100.1 (mRNA capping enzyme family protein )

HSP 1 Score: 627.9 bits (1618), Expect = 9.3e-180
Identity = 307/460 (66.74%), Postives = 370/460 (80.43%), Query Frame = 0

Query: 1   MDLNASPVPEEDEETFDRHEVAYSAPEDHIETGASIARREREERKRRLKRDRSIERPVHE 60
           MDLNASP PEED+E + RH   YS+ ++ IE+   IARREREERK+R++ D    +P H 
Sbjct: 5   MDLNASPQPEEDDEPYVRHLEDYSSRDERIESAVEIARREREERKKRMRYD----KPTHN 64

Query: 61  YQQPARDQSYPAKNQKTV-RSRLPPGWLDCPAFGQEICCMIPSKVPLGDTFSDCVAPGKR 120
            Q   RDQ Y  +N K   R ++P GWLDCP  G EI  ++PSKVPL +++++ V PG R
Sbjct: 65  SQPVFRDQYYQNRNTKAYDRYKIPQGWLDCPPSGNEIGFLVPSKVPLNESYNNHVPPGSR 124

Query: 121 YAFKQAIHQQRVWGRKLGLVIDLTNSYRYYTTTDLNKEGIKYVKIPCKGRDSVPDNKSVN 180
           Y+FKQ IH QR+ GRKLGLVIDLTN+ RYY+TTDL KEGIK+VKI CKGRD+VPDN SVN
Sbjct: 125 YSFKQVIHNQRIAGRKLGLVIDLTNTTRYYSTTDLKKEGIKHVKIACKGRDAVPDNVSVN 184

Query: 181 MFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARALSISVTQALKMFSDARPP 240
            FV EV+QF+   KHSKKYILVHCTHGHNRTG+MI++YL R+  ++VTQALK+FSDARPP
Sbjct: 185 AFVNEVNQFVLNLKHSKKYILVHCTHGHNRTGFMIVHYLMRSGPMNVTQALKIFSDARPP 244

Query: 241 GIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEAVPDD-DDDGGSAAPLNE 300
           GIYKPDYIDALY+FYHE KPE V+ P TPEWKRS++LDLNGEA+PDD DDDGG A P+  
Sbjct: 245 GIYKPDYIDALYSFYHEIKPESVICPSTPEWKRSTELDLNGEALPDDEDDDGGPAGPVQG 304

Query: 301 NHDDAAQV---MTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARANLQFPGSHPVSLNR 360
             +++ QV   M+NDD+LGDEIP DQE  ++ F Y+ML +N G R   QFPGSHPVSLNR
Sbjct: 305 FQEESHQVDVKMSNDDVLGDEIPPDQEEGYRQFFYRMLSLNIGGRGCSQFPGSHPVSLNR 364

Query: 361 ENLQLLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRFPYRNANDGLVEKI 420
           ENLQLLRQRYYYATWKADGTRYMML+T DGCY++DR F+FRRVQMRFP+R+  +G+ +K+
Sbjct: 365 ENLQLLRQRYYYATWKADGTRYMMLLTTDGCYIVDRSFRFRRVQMRFPFRHPTEGISDKV 424

Query: 421 HNYTLLDGEMIIDTLPDSQKQERRYLIYDMMAINHVSVTE 456
           H++TLLDGEMIIDTLPD QKQERRYLIYDM+AIN  SV E
Sbjct: 425 HHFTLLDGEMIIDTLPDKQKQERRYLIYDMVAINGQSVVE 460

BLAST of Cp4.1LG10g08270 vs. TAIR 10
Match: AT5G28210.1 (mRNA capping enzyme family protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.9e-172
Identity = 339/640 (52.97%), Postives = 428/640 (66.88%), Query Frame = 0

Query: 45  KRRLKRDRSIERPVHEYQQPARDQSYPAKNQKTVRSRLPPGWLDCPAFGQEICCMIPSKV 104
           KR L+ DR+  R   E+ +  R  S+  ++QK     +P GWLDCP FGQ I  +IPSKV
Sbjct: 19  KRHLE-DRTENR--EEHSKRLRPTSF--RDQKVT---IPQGWLDCPRFGQHIGLIIPSKV 78

Query: 105 PLGDTFSDCVAPGKRYAFKQAIHQQRVWGRKLGLVIDLTNSYRYY-TTTDLNKEGIKYVK 164
           PL ++++DCV  GKRY FKQ       W  K+GLVIDLTN+ RYY   T+L +  I+YVK
Sbjct: 79  PLSESYNDCVPSGKRYNFKQ-------WLTKIGLVIDLTNTTRYYHPNTELRQNRIEYVK 138

Query: 165 IPCKGRDSVPDNKSVNMFVYEVSQFISRQKHSKKYILVHCTHGHNRTGYMIINYLARAL- 224
           I C GRDSVPDN SVN FV+EV+QF      S+KY+LVHCTHGHNRTG+MI++YL R+  
Sbjct: 139 IRCSGRDSVPDNVSVNTFVHEVTQF-ENHNLSEKYLLVHCTHGHNRTGFMIVHYLMRSRP 198

Query: 225 SISVTQALKMFSDARPPGIYKPDYIDALYAFYHEKKPELVVYPPTPEWKRSSDLDLNGEA 284
            +SVTQALK+FSDARPPGIYKPDYIDALY FYHE KP  V+ PPTPEWKRS       EA
Sbjct: 199 MMSVTQALKIFSDARPPGIYKPDYIDALYRFYHEVKPGSVICPPTPEWKRSE------EA 258

Query: 285 VPDDDDDGGSAAPLNENHDDAAQVMTNDDILGDEIPEDQERAFKHFCYQMLKMNAGARAN 344
             DDDD+  S   +  N+ +    ++NDD+LGDEIP DQE ++++    ML +      +
Sbjct: 259 KFDDDDNALSYREVQGNNQEENVQLSNDDVLGDEIPYDQEVSYQNSINHMLNI------S 318

Query: 345 LQFPGSHPVSLNRENLQLLRQRYYYATWKADGTRYMMLITMDGCYLIDRGFKFRRVQMRF 404
           +QFPGSHP+SL RE LQLLRQRYYYATWKADGTRYMML+T DGCYL++R ++FRRVQMRF
Sbjct: 319 MQFPGSHPLSLGREALQLLRQRYYYATWKADGTRYMMLLTRDGCYLVNREYRFRRVQMRF 378

Query: 405 PYRNANDGLVEKIHNYTLLDGEMIIDTLP--DSQKQERRYLIYDMMAINHVSVTERPFYE 464
           P          K+H+YTLLDGEM++DT+   ++Q+  RRYL+YD++AIN   V ERPF E
Sbjct: 379 PCEYEPSDY--KVHHYTLLDGEMVVDTIKEGETQRHVRRYLVYDLVAINGQFVAERPFSE 438

Query: 465 RWKMLEKEVIEPRNYERQNIYQSRNPQYRYDLEPFRVRRKDFWLLSTV-TKLLKEFIPRL 524
           RW +LE+E+I+PRN E+    + R+  YRYD EPF VR K F LLS V  K+  E IP L
Sbjct: 439 RWNILERELIKPRNDEK----KVRDHWYRYDKEPFGVRIKAFCLLSAVEKKVFNELIPLL 498

Query: 525 SHDADGLIFQMGDD----------------------------DSQTLILFERGKRKTMEG 584
           SH++DGLIFQ  D+                              Q L L ERG+ K MEG
Sbjct: 499 SHESDGLIFQGWDNPYVFGPNKDLLKWKFVETLDFLFDMDKYGRQMLFLQERGRMKLMEG 558

Query: 585 NRVKFK----DGDPSFYSGKIVECSWDSDERVWVCMRIRTDKTTPNDFNTYRKVMRSIKD 644
             V+F+    D D + Y GKIVECSWD +++VWV +RIR DK+ PN   T R V++ I+D
Sbjct: 559 FAVEFRGDGWDNDLASYCGKIVECSWDKEKKVWVSLRIRVDKSKPNGIGTGRSVIKCIED 618

Query: 645 NITEEDLLKEIHEIIRLPMYADRIRNDSKAAQHTNSTRRR 648
           ++T+E LLKEI EII LPMY +RI ND+K A       R+
Sbjct: 619 DLTKEVLLKEIKEIILLPMYVERIENDTKEAARRKHGNRK 624