Cp4.1LG10g07910 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g07910
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionVacuolar cation/proton exchanger
LocationCp4.1LG10: 5356035 .. 5360453 (-)
RNA-Seq ExpressionCp4.1LG10g07910
SyntenyCp4.1LG10g07910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGCCTATAAAGAGTTCCATCTTACCGCCATATCTCTCAGAAAACATCTGAGACCAACAGCGCCAACTCTATCTTCCTCGTCCCTTTTTCCAGCCTCAAAAAAAGCGAACCCATGGCTTCCCAGTCCGAGCCATGGCTCATGGAAAACGGAACCCTTAAGCGCTTAACCAAGGAGAGTCGCCACGGCCATGGCCGAACTGCCCACAACTTGTCCTCCTCTTCCCTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGCAAGTTCCTCATCAATCTTCAGGAGGTCATTTTGGGAACTAAACTTTCCATTCTCTTCCCTGCCATTCCGCTCGCTATTGTCGCTCAGAGTTTCGGGTTTGGAAGAGTGAGAACCCTTATCCCCTCTCTATTTCCCGTGAAACGACATGTCGTTCTGGTTAATTTAATTAATTAACTAATTTATTTATGTATTTAGCCGTGGATTTTTGCTTTGAGCTTGCTTGGCCTCACGCCATTGGCTGAACGTGTCAGCTTCCTGACCGAGTAAGCTTCTCTTAACCTTTTTATTAATATCAAATATTTATTATTATTCTTTAATTTCCTCTTATTGTTTCCTTTCGTCTTTGAAGGCAAATCGCTTATTTCACCGGCCCAACAGGTAATCGGTCGTTTATATTAATTTTTTTTTTTTTAATAATAATAAAAAAAAGATTTAATTAAAAATTTGGTATATAGTTGGGGGGCTTTTGAATGCAACATGTGGGAATGCGACGGAGCTGATAATTGCAATTCTGGCGCTGACACAAGGAAAGATAGACGTGGTGAAGTTCTCTCTTTTGGGCTCTGTTCTTTCCAACCTTCTTCTTGTTCTTGGCACCTCTCTCTTCTGTGGTGGCATTATTAATAAAGAGCAGACTTATGACAGAGTAAGTAACACCTCTCTCTCTCTCTCTCGTTATATTAATTTTTTACGAACCATCTAAGTTCACCGCTAACACATATTGTTATATTTGGCTTTTCTTTCTGAGCTTACTCTCAAAGTTTTAAAACATGTTTTTACTGTGAGATCTCAAAGTTAGTTGGAGAAGTAACAGATACATTTTAAAGCTTTAGGAAAAGACTAGGAGAGAAAGCCGGACCTAAATAAGATCATACAAGTCAGAGAGGAGAACAAATGATATGAAAACCTCTGTCTAACAAACCTGTTTTAAAATATTGGGAGAGAGGTTAAAGAAGACAATATATCTAACGGTGTAGTTGAACTATTATAATAATATCATAACTGGATAATGGACCCAATGAGTGGAGGGTGTGAAAATCTGTTATTGCTATTGGATGATAAAAGTCCCACATCGACTAATTTAGGGAATGATCATGAGTTTATAATTAAGAAATATCTCTTCATTGGTATAAGGCATTTTGGAGAAGCGGAAAGGAAAGTGAATTTTAAACCGGATTATTAGGTGAACGGTAATAGATTTGAAGTCTTAGAAATATAATTAAATATGTAATTTTGTAATTTTAACAGAGGCAAGCGGATGTGAACGCACTAATGCTGATGCTGTCATTACTGTGCCACCTATTGCCGTTATTGTTTGGGTACGCCGCCTCCTCCGCCTCCTCCGCCTCCACTGCCGCCGCCTCCACCCTCCACTTGTCCAGAGCCAGCAGCATTGTAATGCTCGCCGCTTACATTGCTTACTTGGTCTTCCAACTCTGGACTCACCGGACATTATTTGAAACTGAGGTACGTTCCTTCTCCTCTCTTTAATATTCTATTTTTAATTTAAAAAAATTCAGTTATTTATTTATTTATTTATTTAAATAATTTAAGTTTATAAACACCATTTCTATCCATCATTATTATTATTATTATTTAATTTTCAAAATTAAATCAGGTAAAAAGTTAGTACACTATAATTATAAGAAGGTGATGTATTTGTCACACTGACTAGTGGCTAAGGATGTTTTGGTGGTTCTAAATGAACCACTACGACTACGACCACGGCCACGACAGCAACAACAAAAGTCGACTTTATCGGAAAAATGCTATGGTCCCGAATCTTCCACTAGAATAGTCGTTTATCTACTAATAAATATTCAAATTTAGTTTCAAATTTTTAATAGATCGTAAAAATAGTCCCGACTATAAATTGGTTTCGTATTTTATAAAATTAAATAAAAATAACTAATTTTAAGATTTTATTAAAATTACACACAATAATTATAATTTTTAAGATTTTATTAAAATTACACACAATAATTATAATAATAATAACCAATGTCTTCGTGGGTTGATTTTTGAACCAAGATAAACATTTATTTATTAAAATTGAAATGTTTTATTTATTTATTTTATTGTGTGAGAATTAAATTATTTAATTATATTATTGGAAAATTAGGAAACCGAAGACGACGACGTTTCTGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGCTGGAATGACACTTCTTATTGCTTTGCTGTCCGAATACGTTGTCAACACAATTGAGGTAAATTTTAATTACTGTATATTTAAAGGGCTTTTCAGCAAAAAATGAAATATATTATTATTTTTTTCTCTCTTTAATAAAAAATTAAAATTGGGATAATTATAATTTTTTATAAACTAAAGTTTATTATTAATTTCAGGAAGCATCTACTACTTGGGGTTTGTCGGTCAGTTTCCTTAGTATAATCTTGCTCCCAATTGTCGGAAATGCAGCCGAACACGCCGGAGCTATCATATTCGCCTTCAAGAATAAGTTGGTAAGTATCGTAAATTTTCACGTATAAGTAGTTCAATTAATTTTAAAAAAATTAAATTTTTCGAGATTTATTAAATATAAAATTAAATGTTTTGTTTTTGTTTACAGGACATATCATTGGGAGTTGCATTGGGATCTGCAACACAAATTGCACTTTTTGTGGTACGTATGTGTAATTAATGAAAACTTTAAACCCCTCTTAGAAAAAGGTTGAAATTGCACCAACTGACCCCTGAAGAATATGTAATTGCAGATTCCACTATGTGTGATAGTTGCTTGGACAATGGGAGTGGACATGGACCTGAACTTTAACCTCATGGAAACGGGGTCGCTTGGGTTGGCCATTATTGCCACGGCCTTCACTTTACAAGATGGAACCTCTCACTATATTAAAGGCCTCATTCTTCTCCTCTGCTACATTATCATTGCTGCTTGCTTCTTTGTTTCCAAGACTCAAGAAGGTGAGTGACTAAATTGCATGGTTCGGGTTCGGTTCGAGTGAGTTTAGTTCATGGGTTTCAATAGTCTATTTTTGTGTAGTTTTAGGGTTAAGAAGGGTTTGAATTGTGTGAATTCGTGTAGAGGGGAGTGCCAGCGAGAACGCTGGGCTTCGAAGGGAGATGGATTGTAAGATCCCACGTCGGTTGGAGAAGATCGTGAAAACCTCTCCCTTGTATACGTGTTTTAACAACTTTGATGGGAAGCCCGAAAGGGAAACCTCAAAGAGGACAATATAGCGGTAGATTTTGGTTTAAAAACTTTGAGAGGAAACCCAAAAGCAAAACCCTAAAGAGAACAATATCTGCGAGCGTTGGGTTTGGGTTAAAAACTTTGAGAGGAAGCCTCAAAAGCAAAACCTTAAACAGAACAATATCTGGTAGGGGTGAGTTTGGGTTTAAAAACTTTGAAAGATAGTTGGAAAGGAAAACCCTAAAGACAATATCTGTTAGGGGTGGGTTCGGGCTCTTATATGATGAGTACTTTTAACAAAAGTATCTGCTAGGGTGGGTTTGGGCTCTTACATGAGTACTTTTAATGGAAGTACTTCAATTAGGTATCGGGTCGAATAATTAGGTTAATTTTGGGCTCTTACATGAGTACTTTTAATGAAAGTACTTCAATTAGGTATCGGGTCGAGTAATTAGGTCATTAAATTAACGTATTTTTGTTTTTCGCCCTTGCAGATGACAACAATGCCCCCAACTTGGGACTCCAATCATCAAGCCAATCAATCTTCAGAGCTTAAAGGCTAATATGATGCTTTTAAGTGGGTCAAATTGCAAAATGAGAGAGGGGTTACTTTTGAAATCTGGTCATGAAGAAGGACCAAACAGCAAAATCAAATGGATTTGTTATTAAATTCTTTGATATCAGAATTGGCCTGGATCGAACGCATGCCGCCTGCCTGGTGGAAAGCTTAGAAAATTTCAAACTATGGGGAGTTTGATGAAGGCAGTTTGTGGGCTAATAATTAATCGCATGCAATCTGCAATAAATATTTTTATGCTTTTTTTAATTTGTGTTTTTATATGCATTTTTCGTCGTTGTGTTTGAATTTTCATGTATCATAAGAATATGTCGGTAATGAGAATCATATCATTTCTATTTTCCTCTCCTTTTTAATTATACTTTAAA

mRNA sequence

TCAGCCTATAAAGAGTTCCATCTTACCGCCATATCTCTCAGAAAACATCTGAGACCAACAGCGCCAACTCTATCTTCCTCGTCCCTTTTTCCAGCCTCAAAAAAAGCGAACCCATGGCTTCCCAGTCCGAGCCATGGCTCATGGAAAACGGAACCCTTAAGCGCTTAACCAAGGAGAGTCGCCACGGCCATGGCCGAACTGCCCACAACTTGTCCTCCTCTTCCCTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGCAAGTTCCTCATCAATCTTCAGGAGGTCATTTTGGGAACTAAACTTTCCATTCTCTTCCCTGCCATTCCGCTCGCTATTGTCGCTCAGAGTTTCGGGTTTGGAAGACCGTGGATTTTTGCTTTGAGCTTGCTTGGCCTCACGCCATTGGCTGAACGTGTCAGCTTCCTGACCGAGCAAATCGCTTATTTCACCGGCCCAACAGTTGGGGGGCTTTTGAATGCAACATGTGGGAATGCGACGGAGCTGATAATTGCAATTCTGGCGCTGACACAAGGAAAGATAGACGTGAGGCAAGCGGATGTGAACGCACTAATGCTGATGCTGTCATTACTGTGCCACCTATTGCCGTTATTGTTTGGGTACGCCGCCTCCTCCGCCTCCTCCGCCTCCACTGCCGCCGCCTCCACCCTCCACTTGTCCAGAGCCAGCAGCATTGTAATGCTCGCCGCTTACATTGCTTACTTGGTCTTCCAACTCTGGACTCACCGGACATTATTTGAAACTGAGGAAACCGAAGACGACGACGTTTCTGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGCTGGAATGACACTTCTTATTGCTTTGCTGTCCGAATACGTTGTCAACACAATTGAGGAAGCATCTACTACTTGGGGTTTGTCGGTCAGTTTCCTTAGTATAATCTTGCTCCCAATTGTCGGAAATGCAGCCGAACACGCCGGAGCTATCATATTCGCCTTCAAGAATAAGTTGGACATATCATTGGGAGTTGCATTGGGATCTGCAACACAAATTGCACTTTTTGTGATTCCACTATGTGTGATAGTTGCTTGGACAATGGGAGTGGACATGGACCTGAACTTTAACCTCATGGAAACGGGGTCGCTTGGGTTGGCCATTATTGCCACGGCCTTCACTTTACAAGATGGAACCTCTCACTATATTAAAGGCCTCATTCTTCTCCTCTGCTACATTATCATTGCTGCTTGCTTCTTTGTTTCCAAGACTCAAGAAGATGACAACAATGCCCCCAACTTGGGACTCCAATCATCAAGCCAATCAATCTTCAGAGCTTAAAGGCTAATATGATGCTTTTAAGTGGGTCAAATTGCAAAATGAGAGAGGGGTTACTTTTGAAATCTGGTCATGAAGAAGGACCAAACAGCAAAATCAAATGGATTTGTTATTAAATTCTTTGATATCAGAATTGGCCTGGATCGAACGCATGCCGCCTGCCTGGTGGAAAGCTTAGAAAATTTCAAACTATGGGGAGTTTGATGAAGGCAGTTTGTGGGCTAATAATTAATCGCATGCAATCTGCAATAAATATTTTTATGCTTTTTTTAATTTGTGTTTTTATATGCATTTTTCGTCGTTGTGTTTGAATTTTCATGTATCATAAGAATATGTCGGTAATGAGAATCATATCATTTCTATTTTCCTCTCCTTTTTAATTATACTTTAAA

Coding sequence (CDS)

ATGGCTTCCCAGTCCGAGCCATGGCTCATGGAAAACGGAACCCTTAAGCGCTTAACCAAGGAGAGTCGCCACGGCCATGGCCGAACTGCCCACAACTTGTCCTCCTCTTCCCTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGCAAGTTCCTCATCAATCTTCAGGAGGTCATTTTGGGAACTAAACTTTCCATTCTCTTCCCTGCCATTCCGCTCGCTATTGTCGCTCAGAGTTTCGGGTTTGGAAGACCGTGGATTTTTGCTTTGAGCTTGCTTGGCCTCACGCCATTGGCTGAACGTGTCAGCTTCCTGACCGAGCAAATCGCTTATTTCACCGGCCCAACAGTTGGGGGGCTTTTGAATGCAACATGTGGGAATGCGACGGAGCTGATAATTGCAATTCTGGCGCTGACACAAGGAAAGATAGACGTGAGGCAAGCGGATGTGAACGCACTAATGCTGATGCTGTCATTACTGTGCCACCTATTGCCGTTATTGTTTGGGTACGCCGCCTCCTCCGCCTCCTCCGCCTCCACTGCCGCCGCCTCCACCCTCCACTTGTCCAGAGCCAGCAGCATTGTAATGCTCGCCGCTTACATTGCTTACTTGGTCTTCCAACTCTGGACTCACCGGACATTATTTGAAACTGAGGAAACCGAAGACGACGACGTTTCTGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGCTGGAATGACACTTCTTATTGCTTTGCTGTCCGAATACGTTGTCAACACAATTGAGGAAGCATCTACTACTTGGGGTTTGTCGGTCAGTTTCCTTAGTATAATCTTGCTCCCAATTGTCGGAAATGCAGCCGAACACGCCGGAGCTATCATATTCGCCTTCAAGAATAAGTTGGACATATCATTGGGAGTTGCATTGGGATCTGCAACACAAATTGCACTTTTTGTGATTCCACTATGTGTGATAGTTGCTTGGACAATGGGAGTGGACATGGACCTGAACTTTAACCTCATGGAAACGGGGTCGCTTGGGTTGGCCATTATTGCCACGGCCTTCACTTTACAAGATGGAACCTCTCACTATATTAAAGGCCTCATTCTTCTCCTCTGCTACATTATCATTGCTGCTTGCTTCTTTGTTTCCAAGACTCAAGAAGATGACAACAATGCCCCCAACTTGGGACTCCAATCATCAAGCCAATCAATCTTCAGAGCTTAA

Protein sequence

MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLNATCGNATELIIAILALTQGKIDVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAACFFVSKTQEDDNNAPNLGLQSSSQSIFRA
Homology
BLAST of Cp4.1LG10g07910 vs. ExPASy Swiss-Prot
Match: Q93Z81 (Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 2.1e-142
Identity = 291/452 (64.38%), Postives = 337/452 (74.56%), Query Frame = 0

Query: 1   MASQSEPW--LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGC 60
           M S  EPW  + ENG    T K  ++E R  HGRTAHN+SSSSLRKKSDL LV K+    
Sbjct: 1   MGSIVEPWAAIAENGNANVTAKGSSRELR--HGRTAHNMSSSSLRKKSDLRLVQKVPCKT 60

Query: 61  LRKFLINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTE 120
           L+  L NLQEVILGTKL++LF AIPLAI+A S+ +GRP IF LSL+GLTPLAERVSFLTE
Sbjct: 61  LKNILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTE 120

Query: 121 QIAYFTGPTVGGLLNATCGNATELIIAILALTQGKI------------------------ 180
           Q+A++TGPTVGGLLNATCGNATELIIAILAL   K+                        
Sbjct: 121 QLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLF 180

Query: 181 -------------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSR 240
                        D +QADVN  +L++ LLCHLLPLL  YAA+   S S     +L LSR
Sbjct: 181 FGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSR 240

Query: 241 ASSIVMLAAYIAYLVFQLWTHRTLFETEETE----DDDVSGEEA-VIGFWSAFAWLAGMT 300
            SSIVML AYIAYL+FQLWTHR LFE ++ +    DD+VS EE  VIGFWS FAWL GMT
Sbjct: 241 TSSIVMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMT 300

Query: 301 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360
           ++IALLSEYVV+TIE+AS +WGLSVSF+SIILLPIVGNAAEHAGAIIFAFKNKLDISLGV
Sbjct: 301 IVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360

Query: 361 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 405
           ALGSATQI+LFV+PL VIVAW +G+ MDLNFN++ET SL LAII TAFTLQDGTSHY+KG
Sbjct: 361 ALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKG 420

BLAST of Cp4.1LG10g07910 vs. ExPASy Swiss-Prot
Match: Q39253 (Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 SV=3)

HSP 1 Score: 493.4 bits (1269), Expect = 2.4e-138
Identity = 280/451 (62.08%), Postives = 328/451 (72.73%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKI----------------------------- 184
           TGPT+GGLLNATCGNATELIIAILALT  K+                             
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 --------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
                   D +QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCY 411
           I LFV+PL +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGL+LLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

BLAST of Cp4.1LG10g07910 vs. ExPASy Swiss-Prot
Match: Q769E5 (Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1a PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 9.7e-127
Identity = 261/444 (58.78%), Postives = 315/444 (70.95%), Query Frame = 0

Query: 10  MENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINLQEVILGT 69
           ME G  ++L     HG  RTAHN+SSSSLRKKSD  LV K+    LR  L NLQEV L T
Sbjct: 7   MEAG--RKLAARHPHGRSRTAHNMSSSSLRKKSDAALVRKVPVAPLRPLLANLQEVFLAT 66

Query: 70  KLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLN 129
           KL++LFPA+PLAI AQ F F + W+FALSLLGL PLAERVSFLTEQIA +TGPTVGGLLN
Sbjct: 67  KLAVLFPAVPLAIAAQCFRFDQVWVFALSLLGLIPLAERVSFLTEQIALYTGPTVGGLLN 126

Query: 130 ATCGNATELIIAILALTQGKIDV------------------------------------- 189
           ATCGNATELIIA+ AL +GKI+V                                     
Sbjct: 127 ATCGNATELIIALFALLKGKIEVVKCSLLGSVLSNLLLVLGTSLFCGGVVNLGARQPYDR 186

Query: 190 RQADVNALMLMLSLLCHLLPLLFGY--AASSASSASTAAASTLHLSRASSIVMLAAYIAY 249
            Q+DV+  +L L++LCH  PLL  Y  AA   S ++T+AA++L LSRA S VMLA+Y+AY
Sbjct: 187 NQSDVSTALLFLAVLCHSAPLLLRYAVAAGEHSVSATSAAASLDLSRACSFVMLASYVAY 246

Query: 250 LVFQLWTHRTLFETEETEDDDV--SGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNTIEE 309
           L FQL THR LFE +E +  D     EE  +GF SA  WLA MT +I++LSEYVV TIE 
Sbjct: 247 LFFQLKTHRQLFEPQEVDGGDAGDDDEEPALGFASALFWLALMTAVISVLSEYVVGTIEP 306

Query: 310 ASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPLC 369
            S +WGLSVSF+SIILLPIVGNAAEHAGAIIFA KNKLDI+LGVALGSATQI++FV+PL 
Sbjct: 307 TSQSWGLSVSFISIILLPIVGNAAEHAGAIIFALKNKLDITLGVALGSATQISMFVVPLS 366

Query: 370 VIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAACFFV 411
           V+VAW MGV MDL+F L+ETGSL +A++ TAFTLQDGTSHY+KG++LLLCYI+I ACFFV
Sbjct: 367 VLVAWIMGVQMDLDFKLLETGSLFMAVLVTAFTLQDGTSHYLKGILLLLCYIVIGACFFV 426

BLAST of Cp4.1LG10g07910 vs. ExPASy Swiss-Prot
Match: Q5TKG3 (Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1b PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 8.8e-120
Identity = 245/428 (57.24%), Postives = 300/428 (70.09%), Query Frame = 0

Query: 9   LMENGTLKRLTKESRH-----GHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINLQ 68
           +ME+ T+ +      H        RTAHNLSSSSLRK SD +L+ K+    LR  L NL 
Sbjct: 7   MMESNTMAKEMAVRHHLLPGSPRRRTAHNLSSSSLRKSSDASLLHKVPCAALRSLLANLN 66

Query: 69  EVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPT 128
           +V+L T+L +LFPA+ LAI A    FG+ W+F LSL+GL PLAER+SFLTEQIA++TGPT
Sbjct: 67  DVLLTTRLFLLFPAVLLAIAATYLHFGQVWVFVLSLIGLVPLAERLSFLTEQIAFYTGPT 126

Query: 129 VGGLLNATCGNATELIIAILALTQGKI--------------------------------- 188
           VGGLLNAT GN TE+IIA+LAL +GKI                                 
Sbjct: 127 VGGLLNATFGNVTEVIIALLALREGKIEVVKCSLLGSILSNLLLVLGTSLFLAGIANLRA 186

Query: 189 ----DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLAA 248
               D +QA VN  +LML++LCH LPL+  YA +S   A  +  + LHLSRA SI+ML A
Sbjct: 187 HQPYDTKQAHVNTALLMLAVLCHSLPLMLRYAVTSGDHAIVSGDAALHLSRACSILMLIA 246

Query: 249 YIAYLVFQLWTHRTLFETEETEDDD------VSGEEAVIGFWSAFAWLAGMTLLIALLSE 308
           Y+AYL FQL THR LFE ++ EDDD         +E V+GF SA  WLA MTLL ALLS 
Sbjct: 247 YLAYLFFQLNTHRQLFEPQQVEDDDDDDLVIAQDDEPVLGFSSAMIWLALMTLLTALLSG 306

Query: 309 YVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQI 368
           YVV+TIE AS +W LSVSF+SIILLPIVGNAAEHAGA+IFA KNK+DI+LGV+LGSATQI
Sbjct: 307 YVVSTIEAASESWELSVSFISIILLPIVGNAAEHAGAVIFALKNKMDITLGVSLGSATQI 366

Query: 369 ALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYI 389
           ++FV+P+ VIVAWTMG+ MDL+FNL+ETGSL LAI+ TAFTLQ+G SHY+KGLIL+LCY 
Sbjct: 367 SMFVVPVSVIVAWTMGIPMDLDFNLLETGSLFLAILVTAFTLQEGESHYLKGLILVLCYA 426

BLAST of Cp4.1LG10g07910 vs. ExPASy Swiss-Prot
Match: Q945S5 (Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 SV=2)

HSP 1 Score: 411.8 bits (1057), Expect = 9.4e-114
Identity = 237/439 (53.99%), Postives = 309/439 (70.39%), Query Frame = 0

Query: 3   SQSEPWLMEN---GTLKRLTKESRHGHGRTAHNLSSSSL-RKKSDLTLVSKIQFGCLRKF 62
           S S   L+EN   G+ K   + SR    R    +S+SSL RK+SDL L+S++++  +R+ 
Sbjct: 8   SSSNLSLLENGGGGSDKPTAETSR----RVRRTVSASSLIRKRSDLKLISRVRWEFMRRI 67

Query: 63  LINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAY 122
           L NLQEV+LGTKL ILFPA+PLA+VA  +   R W+FALSLLGLTPLAER+SFLTEQIA+
Sbjct: 68  LTNLQEVLLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAF 127

Query: 123 FTGPTVGGLLNATCGNATELIIAILALTQGKI---------------------------- 182
            TGPTVGGL+NATCGNATE+IIAILA+ Q K+                            
Sbjct: 128 HTGPTVGGLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGI 187

Query: 183 ---------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSI 242
                    D RQ D+N+++L L+LLC  LP++  +   +  +     +  + LSRASS 
Sbjct: 188 SNLRKHQSFDPRQGDMNSMLLYLALLCQTLPMIMRF---TMEAEEYDGSDVVVLSRASSF 247

Query: 243 VMLAAYIAYLVFQLWTHR------TLFETEETEDDDVSGEE---AVIGFWSAFAWLAGMT 302
           VML AY+A+L+F L++         L + E+  DDDVS +E   AVIG WSA  WL  MT
Sbjct: 248 VMLIAYLAFLIFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMT 307

Query: 303 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 362
           LL+ALLS+Y+V+TI++A+ +WGLSV F+ IILLPIVGNAAEHAGA+IFAF+NKLDI+LG+
Sbjct: 308 LLVALLSDYLVSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGI 367

Query: 363 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 392
           ALGSATQIALFV+P+ V+VAWTMG++MDLNFNL+ET    L+I+ T+  LQDGTS+Y+KG
Sbjct: 368 ALGSATQIALFVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKG 427

BLAST of Cp4.1LG10g07910 vs. NCBI nr
Match: XP_023545142.1 (vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761 bits (1965), Expect = 1.03e-275
Identity = 413/448 (92.19%), Postives = 413/448 (92.19%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATELIIAILALTQGKIDV                            
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 -------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
                  RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQTYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 413
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

BLAST of Cp4.1LG10g07910 vs. NCBI nr
Match: XP_022978169.1 (vacuolar cation/proton exchanger 3 [Cucurbita maxima])

HSP 1 Score: 759 bits (1960), Expect = 5.96e-275
Identity = 411/448 (91.74%), Postives = 413/448 (92.19%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATE+IIAILALTQGKIDV                            
Sbjct: 121 GPTVGGLLNATCGNATEMIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 -------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
                  RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 413
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

BLAST of Cp4.1LG10g07910 vs. NCBI nr
Match: XP_022949765.1 (vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >KAG6603584.1 Vacuolar cation/proton exchanger 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 759 bits (1959), Expect = 8.46e-275
Identity = 411/448 (91.74%), Postives = 413/448 (92.19%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATELIIAILALTQGKIDV                            
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 -------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
                  RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 413
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

BLAST of Cp4.1LG10g07910 vs. NCBI nr
Match: XP_008449925.1 (PREDICTED: vacuolar cation/proton exchanger 3 [Cucumis melo])

HSP 1 Score: 721 bits (1861), Expect = 6.90e-260
Identity = 391/450 (86.89%), Postives = 400/450 (88.89%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIP AIVA SFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPFAIVAHSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDV                            
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 ---------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
                    RQADVN+LMLMLSLLCHLLPLLFGYAASSAS   TAAASTLHLSRASSIVM
Sbjct: 181 LRREQIYDRRQADVNSLMLMLSLLCHLLPLLFGYAASSAS---TAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L+AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTLLIALLSEYVV
Sbjct: 241 LSAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 413
           VIPLCVIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

BLAST of Cp4.1LG10g07910 vs. NCBI nr
Match: NP_001267681.1 (vacuolar cation/proton exchanger 1-like [Cucumis sativus] >XP_011652900.1 vacuolar cation/proton exchanger 1-like isoform X1 [Cucumis sativus] >AFQ99295.1 cation exchanger CAX3 [Cucumis sativus] >KGN54380.1 hypothetical protein Csa_018138 [Cucumis sativus])

HSP 1 Score: 721 bits (1860), Expect = 9.79e-260
Identity = 390/450 (86.67%), Postives = 401/450 (89.11%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDV                            
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 ---------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
                    RQADVN+LMLMLSLLCHLLPLLFGYAA+SAS   TAAASTLHLSRASSIVM
Sbjct: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASAS---TAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 413
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

BLAST of Cp4.1LG10g07910 vs. ExPASy TrEMBL
Match: A0A6J1ITD3 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111478234 PE=3 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 2.89e-275
Identity = 411/448 (91.74%), Postives = 413/448 (92.19%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATE+IIAILALTQGKIDV                            
Sbjct: 121 GPTVGGLLNATCGNATEMIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 -------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
                  RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 413
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

BLAST of Cp4.1LG10g07910 vs. ExPASy TrEMBL
Match: A0A6J1GDQ2 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111453055 PE=3 SV=1)

HSP 1 Score: 759 bits (1959), Expect = 4.10e-275
Identity = 411/448 (91.74%), Postives = 413/448 (92.19%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATELIIAILALTQGKIDV                            
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 -------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
                  RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 413
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

BLAST of Cp4.1LG10g07910 vs. ExPASy TrEMBL
Match: A0A1S3BNT2 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103491652 PE=3 SV=1)

HSP 1 Score: 721 bits (1861), Expect = 3.34e-260
Identity = 391/450 (86.89%), Postives = 400/450 (88.89%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIP AIVA SFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPFAIVAHSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDV                            
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 ---------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
                    RQADVN+LMLMLSLLCHLLPLLFGYAASSAS   TAAASTLHLSRASSIVM
Sbjct: 181 LRREQIYDRRQADVNSLMLMLSLLCHLLPLLFGYAASSAS---TAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L+AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTLLIALLSEYVV
Sbjct: 241 LSAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 413
           VIPLCVIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

BLAST of Cp4.1LG10g07910 vs. ExPASy TrEMBL
Match: J7KJQ3 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX3 PE=2 SV=1)

HSP 1 Score: 721 bits (1860), Expect = 4.74e-260
Identity = 390/450 (86.67%), Postives = 401/450 (89.11%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDV---------------------------- 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDV                            
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 ---------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
                    RQADVN+LMLMLSLLCHLLPLLFGYAA+SAS   TAAASTLHLSRASSIVM
Sbjct: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYAAASAS---TAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 413
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

BLAST of Cp4.1LG10g07910 vs. ExPASy TrEMBL
Match: A0A6J1DEY3 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111019448 PE=3 SV=1)

HSP 1 Score: 676 bits (1743), Expect = 3.46e-242
Identity = 371/450 (82.44%), Postives = 394/450 (87.56%), Query Frame = 0

Query: 3   SQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINL 62
           +Q+EPWL+ENGTLKRLTK+SRHGHGRTAHN+SSSSLRKKSDLTLVSKI+F CLR FLINL
Sbjct: 5   TQTEPWLLENGTLKRLTKDSRHGHGRTAHNMSSSSLRKKSDLTLVSKIRFPCLRNFLINL 64

Query: 63  QEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGP 122
           QEVILGTKLSILFPAIPLAI+AQ F FGRPW+FALSLLGLTPLAERVSFLTEQIAYFTGP
Sbjct: 65  QEVILGTKLSILFPAIPLAIIAQIFRFGRPWVFALSLLGLTPLAERVSFLTEQIAYFTGP 124

Query: 123 TVGGLLNATCGNATELIIAILALTQGKIDV------------------------------ 182
           TVGGLLNATCGNATELIIAILALTQ KIDV                              
Sbjct: 125 TVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANMR 184

Query: 183 -------RQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 242
                  RQADVNALMLMLSLLCHLLPLLFGYAASSA+   TAAASTLHLSRASSIVML+
Sbjct: 185 TEQNYDRRQADVNALMLMLSLLCHLLPLLFGYAASSAA---TAAASTLHLSRASSIVMLS 244

Query: 243 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 302
           AYIAYLVFQLWTHR LFE E+ +DD VS EEAVIGF SAFAWL GMT+LIALLSEYVVN+
Sbjct: 245 AYIAYLVFQLWTHRQLFE-EDGDDDVVSDEEAVIGFGSAFAWLIGMTILIALLSEYVVNS 304

Query: 303 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 362
           IEEAST+WGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDI+LGVALGSATQIALFVI
Sbjct: 305 IEEASTSWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDITLGVALGSATQIALFVI 364

Query: 363 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 413
           P+CVIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 365 PMCVIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 424

BLAST of Cp4.1LG10g07910 vs. TAIR 10
Match: AT3G51860.1 (cation exchanger 3 )

HSP 1 Score: 506.9 bits (1304), Expect = 1.5e-143
Identity = 291/452 (64.38%), Postives = 337/452 (74.56%), Query Frame = 0

Query: 1   MASQSEPW--LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGC 60
           M S  EPW  + ENG    T K  ++E R  HGRTAHN+SSSSLRKKSDL LV K+    
Sbjct: 1   MGSIVEPWAAIAENGNANVTAKGSSRELR--HGRTAHNMSSSSLRKKSDLRLVQKVPCKT 60

Query: 61  LRKFLINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTE 120
           L+  L NLQEVILGTKL++LF AIPLAI+A S+ +GRP IF LSL+GLTPLAERVSFLTE
Sbjct: 61  LKNILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTE 120

Query: 121 QIAYFTGPTVGGLLNATCGNATELIIAILALTQGKI------------------------ 180
           Q+A++TGPTVGGLLNATCGNATELIIAILAL   K+                        
Sbjct: 121 QLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLF 180

Query: 181 -------------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSR 240
                        D +QADVN  +L++ LLCHLLPLL  YAA+   S S     +L LSR
Sbjct: 181 FGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSR 240

Query: 241 ASSIVMLAAYIAYLVFQLWTHRTLFETEETE----DDDVSGEEA-VIGFWSAFAWLAGMT 300
            SSIVML AYIAYL+FQLWTHR LFE ++ +    DD+VS EE  VIGFWS FAWL GMT
Sbjct: 241 TSSIVMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMT 300

Query: 301 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360
           ++IALLSEYVV+TIE+AS +WGLSVSF+SIILLPIVGNAAEHAGAIIFAFKNKLDISLGV
Sbjct: 301 IVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360

Query: 361 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 405
           ALGSATQI+LFV+PL VIVAW +G+ MDLNFN++ET SL LAII TAFTLQDGTSHY+KG
Sbjct: 361 ALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKG 420

BLAST of Cp4.1LG10g07910 vs. TAIR 10
Match: AT2G38170.1 (cation exchanger 1 )

HSP 1 Score: 493.4 bits (1269), Expect = 1.7e-139
Identity = 280/451 (62.08%), Postives = 328/451 (72.73%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKI----------------------------- 184
           TGPT+GGLLNATCGNATELIIAILALT  K+                             
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 --------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
                   D +QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCY 411
           I LFV+PL +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGL+LLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

BLAST of Cp4.1LG10g07910 vs. TAIR 10
Match: AT2G38170.3 (cation exchanger 1 )

HSP 1 Score: 491.1 bits (1263), Expect = 8.6e-139
Identity = 275/436 (63.07%), Postives = 320/436 (73.39%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKI----------------------------- 184
           TGPT+GGLLNATCGNATELIIAILALT  K+                             
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 --------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
                   D +QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCY 396
           I LFV+PL +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGL+LLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

BLAST of Cp4.1LG10g07910 vs. TAIR 10
Match: AT5G01490.1 (cation exchanger 4 )

HSP 1 Score: 417.2 bits (1071), Expect = 1.6e-116
Identity = 239/448 (53.35%), Postives = 312/448 (69.64%), Query Frame = 0

Query: 3   SQSEPWLMEN---GTLKRLTKESRHGHGRTAHNLSSSSL-RKKSDLTLVSKIQFGCLRKF 62
           S S   L+EN   G+ K   + SR    R    +S+SSL RK+SDL L+S++++  +R+ 
Sbjct: 8   SSSNLSLLENGGGGSDKPTAETSR----RVRRTVSASSLIRKRSDLKLISRVRWEFMRRI 67

Query: 63  LINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAY 122
           L NLQEV+LGTKL ILFPA+PLA+VA  +   R W+FALSLLGLTPLAER+SFLTEQIA+
Sbjct: 68  LTNLQEVLLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAF 127

Query: 123 FTGPTVGGLLNATCGNATELIIAILALTQGKI---------------------------- 182
            TGPTVGGL+NATCGNATE+IIAILA+ Q K+                            
Sbjct: 128 HTGPTVGGLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGI 187

Query: 183 ---------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSI 242
                    D RQ D+N+++L L+LLC  LP++  +   +  +     +  + LSRASS 
Sbjct: 188 SNLRKHQSFDPRQGDMNSMLLYLALLCQTLPMIMRF---TMEAEEYDGSDVVVLSRASSF 247

Query: 243 VMLAAYIAYLVFQLWTHR------TLFETEETEDDDVSGEE---AVIGFWSAFAWLAGMT 302
           VML AY+A+L+F L++         L + E+  DDDVS +E   AVIG WSA  WL  MT
Sbjct: 248 VMLIAYLAFLIFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMT 307

Query: 303 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 362
           LL+ALLS+Y+V+TI++A+ +WGLSV F+ IILLPIVGNAAEHAGA+IFAF+NKLDI+LG+
Sbjct: 308 LLVALLSDYLVSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGI 367

Query: 363 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 401
           ALGSATQIALFV+P+ V+VAWTMG++MDLNFNL+ET    L+I+ T+  LQDGTS+Y+KG
Sbjct: 368 ALGSATQIALFVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKG 427

BLAST of Cp4.1LG10g07910 vs. TAIR 10
Match: AT2G38170.2 (cation exchanger 1 )

HSP 1 Score: 365.9 bits (938), Expect = 4.2e-101
Identity = 214/351 (60.97%), Postives = 247/351 (70.37%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKI----------------------------- 184
           TGPT+GGLLNATCGNATELIIAILALT  K+                             
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 --------DVRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
                   D +QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISL 311
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKL  S+
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLVSSI 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z812.1e-14264.38Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 ... [more]
Q392532.4e-13862.08Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 ... [more]
Q769E59.7e-12758.78Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q5TKG38.8e-12057.24Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q945S59.4e-11453.99Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023545142.11.03e-27592.19vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo][more]
XP_022978169.15.96e-27591.74vacuolar cation/proton exchanger 3 [Cucurbita maxima][more]
XP_022949765.18.46e-27591.74vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >KAG6603584.1 Vacuo... [more]
XP_008449925.16.90e-26086.89PREDICTED: vacuolar cation/proton exchanger 3 [Cucumis melo][more]
NP_001267681.19.79e-26086.67vacuolar cation/proton exchanger 1-like [Cucumis sativus] >XP_011652900.1 vacuol... [more]
Match NameE-valueIdentityDescription
A0A6J1ITD32.89e-27591.74Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111478234 PE=... [more]
A0A6J1GDQ24.10e-27591.74Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111453055 P... [more]
A0A1S3BNT23.34e-26086.89Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103491652 PE=3 SV... [more]
J7KJQ34.74e-26086.67Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX3 PE=2 SV=1[more]
A0A6J1DEY33.46e-24282.44Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111019448 ... [more]
Match NameE-valueIdentityDescription
AT3G51860.11.5e-14364.38cation exchanger 3 [more]
AT2G38170.11.7e-13962.08cation exchanger 1 [more]
AT2G38170.38.6e-13963.07cation exchanger 1 [more]
AT5G01490.11.6e-11653.35cation exchanger 4 [more]
AT2G38170.24.2e-10160.97cation exchanger 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 244..385
e-value: 9.1E-17
score: 61.4
coord: 93..216
e-value: 2.5E-8
score: 34.0
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 122..388
e-value: 3.1E-17
score: 64.7
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 150..401
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 4..152
NoneNo IPR availablePANTHERPTHR31503:SF1VACUOLAR CATION/PROTON EXCHANGER 3coord: 150..401
NoneNo IPR availablePANTHERPTHR31503:SF1VACUOLAR CATION/PROTON EXCHANGER 3coord: 4..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g07910.1Cp4.1LG10g07910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity