Cp4.1LG10g07220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG10g07220
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	RING-type E3 ubiquitin transferase
Location	Cp4.1LG10: 5977745 .. 5980189 (+)
RNA-Seq Expression	Cp4.1LG10g07220
Synteny	Cp4.1LG10g07220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAATGATTAATTATATGTAATACACGTGTATATCATGGTTTATTTTGACCAAACCGTAGGCGGTTTTGTTTGGCCATGTGGATAACATCCAAAAGTCTTCTCTCTCTCTCTCTCTCTCTCTCTCATATCTTTGTTTTTCCAAGTTTGACTTTCTCTCTCCTTTGACCGACAAAATTATATAAATTAACGACCAAGATCCTCTCCTCTCCGACGGCCTCAATTTACAACTTCCCAACAACTCTCTCTCTCTCTCTCTCTGAATCTCTCTCTAAATCTCTCTCTCTGAATCTCTCTCTAAATCTCTCTCTCTCTCATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTCTGCCCCATCTCTCTCCAAATCATGAAAGACCCCGTCACCGTCGCTTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCAGACAGACACAAGACGTGCCCCGTCACACAGGTCGTCTTGTCAAGCTTCGACATCACTCCCAACCATACTCTAAGACGAGTCATTCAAGCTTGGTGCACTGTCAATGCTTGCAAGGGTATCGAGAGGATTCCGACGCCTAAGCCACCGGTCGATTGTAAGCAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTCCGTTCTCGCAACAGAATTGTCTCCGACGACTCCGATCTATTGCAGCGGGGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCTGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTCAGAAGGTATTATTACTATAATAAATCCAAAATGTTAAAAAAAAAAAATATATATATATATATATATATATAATTATTTGATTTTTTTAAAAAAAATTTAAGCTTATTAAAGTATTAAAAAATAAAAAAAAGATAGCTTTTTTTAAATAAATAATAATAATAAAGGATTCGTTTTTAAATTTTTTTAAAAATTAACTTAAAAAAAATTTAATTTTCAATATTGCCTCGAAACAATTCAAGAAATTTAAAATTTCTTACAATTAATTTTAATTTAAAAAAAAATTAAAATTTTCAGCAATTTTAATAATTTCTTACACGCTATCTAATTATTTAAACCGAATTATTAAATTTTATGACATAAAAATTAATTAATTTATAAAAATTTTGAATATGTCACTAATTTATTAAATAAATAAATAAAAAGTTTAAAAATCTATTAAATTAATATAATTTAAAACTAATTAGAAGAAATATTTTAAATTTTGTGTGCGTTTCAAAAATACATTTTTAATTTTAATTTGCAACGTTTTTCTTTCGTTTTTTTTTTTTTTTAGGGTTTCAAAAATTTCGAGTTTAAATCATTTAAAATTTTAAGCTAAAATTGAAATTTAAACGATTTACGAGTCTAGATCGTTTAGAATTTTAGATCAAAACTGAAATTTAAACGAGTTAGGAGCGATTTCGAACGAAAATTTCAATTTCGATCCTAGAACTCTTATTCTAAATTTGTAATTTGTGTAGTAAAAGTTGAGAATCTAATATTTGATGAACATTGTTGAGCAGATTACGCTTACTTGATTGACGACCAGTTCGCGAATTTGGGGGATGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAGCATATTCAGTAATGCTTCTAACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTATGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACACTCGATCTACTCTGCGGCTGTGCCGACGGTAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTGTTGTATCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAGAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGTCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAGGGTTTGTTTTTGCCCCCAAAACTTTCTTTATTTCAATTGATCGAAATTGGTTTATATATTAAAAAAATTTGTACATATTCTT

mRNA sequence

GAAATGATTAATTATATGTAATACACGTGTATATCATGGTTTATTTTGACCAAACCGTAGGCGGTTTTGTTTGGCCATGTGGATAACATCCAAAAGTCTTCTCTCTCTCTCTCTCTCTCTCTCTCATATCTTTGTTTTTCCAAGTTTGACTTTCTCTCTCCTTTGACCGACAAAATTATATAAATTAACGACCAAGATCCTCTCCTCTCCGACGGCCTCAATTTACAACTTCCCAACAACTCTCTCTCTCTCTCTCTCTGAATCTCTCTCTAAATCTCTCTCTCTGAATCTCTCTCTAAATCTCTCTCTCTCTCATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTCTGCCCCATCTCTCTCCAAATCATGAAAGACCCCGTCACCGTCGCTTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCAGACAGACACAAGACGTGCCCCGTCACACAGGTCGTCTTGTCAAGCTTCGACATCACTCCCAACCATACTCTAAGACGAGTCATTCAAGCTTGGTGCACTGTCAATGCTTGCAAGGGTATCGAGAGGATTCCGACGCCTAAGCCACCGGTCGATTGTAAGCAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTCCGTTCTCGCAACAGAATTGTCTCCGACGACTCCGATCTATTGCAGCGGGGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCTGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTCAGAAGATTACGCTTACTTGATTGACGACCAGTTCGCGAATTTGGGGGATGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAGCATATTCAGTAATGCTTCTAACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTATGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACACTCGATCTACTCTGCGGCTGTGCCGACGGTAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTGTTGTATCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAGAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGTCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAGGGTTTGTTTTTGCCCCCAAAACTTTCTTTATTTCAATTGATCGAAATTGGTTTATATATTAAAAAAATTTGTACATATTCTT

Coding sequence (CDS)

ATGGCAGAATTAGATCAAGAACTTGATCCTCCTCCCTTCTTTCTCTGCCCCATCTCTCTCCAAATCATGAAAGACCCCGTCACCGTCGCTTCCGGCATAACCTACGACCGCGAAAGCATCGAGAAATGGTTGTTTTCAGACAGACACAAGACGTGCCCCGTCACACAGGTCGTCTTGTCAAGCTTCGACATCACTCCCAACCATACTCTAAGACGAGTCATTCAAGCTTGGTGCACTGTCAATGCTTGCAAGGGTATCGAGAGGATTCCGACGCCTAAGCCACCGGTCGATTGTAAGCAAGTGGTTCGAATTCTTGCAGATGCCAAGTTGTCTCCGTTCTCGCAACAGAATTGTCTCCGACGACTCCGATCTATTGCAGCGGGGAGTGAATCTAATAAACGTTGCATGGAAGCTGCTGGAGCTGTGGAGTTCTTGGCTGGAATTGTGTGTAATTCCACCAACGTGGAATTCAGCTCAGAAGATTACGCTTACTTGATTGACGACCAGTTCGCGAATTTGGGGGATGAGGCTTTGAGCATTCTTTACAAACTTCAAATCTCCGAATCCGGCCTGAAATTTCTACTCGCCAACAATGGCGGCATCTTCGTCTCAACCTTAACCAAAATCTTGCAAAACGGAACCTATGGCTCCCGAGCATATTCAGTAATGCTTCTAACTTCAATGTTCGAAGTTGCAGATCAGATTCAGATCCTCAGCCTAAACACCGATTTCTTCGTCGAAATCGTCCAAATTCTGAAGGATCAGATCTCGAAACAGGCTTCGAAATCGGCATTGAAACTGCTAATCGGTCTTTGTTCGAGTTCCAGAAACAGAGTTAAGGCGGTCAAAGCCGGTGCGGTTCCGGTTATGATCGATCTCCTCCTCGATCTCGATTCGTCAGAGAAGCGTTCGTGCGAGATGATTCTCGTGACACTCGATCTACTCTGCGGCTGTGCCGACGGTAGGGCGGAGGTATTGAATCACGCGGCAGGGATCGCTGTTGTATCGAAGAAGATTCTGAGAGTTTCGACACTCGGAAGCGAGAAGGCCGTCGGAATCTTGTGCTCTATCGCGAGATTCTCGGCGAGTGAAAGCGTCGTCCAGGAAATGATGCGGCTCGGAGTTGTGACGAAGCTCTGTTTTCTGCTTCAGGTCGTCGGCGCCGGAGGCAAAGCGAAGGAGAAAGCGAAAGAGATTCTGAAGCTTCATGGTCGGACATGGCGGAATTCTTCATGTTTGCCTTCGAGTCTGCGATCTTCTTTTCCACATTAG

Protein sequence

MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH

Homology

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 3.6e-121
Identity = 236/435 (54.25%), Postives = 309/435 (71.03%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDRESIEKWLFS +  +CPVT+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHTLRR+IQ+WCT+NA  GIERIPTPKPP+   ++ +++ ++  S  +Q  CL+RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAGSESNKRCMEAAGAVEFLAGIVCNST---NVEFSSEDYAYLID-----------DQ 183
            I + + +NKRC+EAA   EFLA IV NS    N   SS   + L D           D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 FANLGDEALSILYKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRAYSVMLLTSM 243
             +L DEALS+LY L  SE+ LK LL N  G   V TLTKI+Q G Y SRAY+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VMIDLLL-DLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC CA+GRAE LNH A IAVVSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVW 420

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.4e-117
Identity = 219/421 (52.02%), Postives = 305/421 (72.45%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR+SIEKWLF+ +  +CPVT+  ++  D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHTLRR+IQ+WCT+NA  G+ERIPTP+PP+   ++ +++ D+  S  +Q  CL+RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSILYK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L DEAL++LY 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC CA+GRAE LNH A IAVV KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQ V  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.7e-60
Identity = 165/457 (36.11%), Postives = 248/457 (54.27%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I KWL  ++  +CPVT+  L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 61  -SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK---LSPFSQQ 120
              D+TPNH LRR+IQ WC  N  +G+ RI TP+ P     VV  + + K        ++
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 121 NCLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLG-D 180
             L++L  +A    +N+R M   G  + L   V     V+ +SED     D +    G D
Sbjct: 121 ETLQKLEVLAMDG-NNRRLMCECGVHKSLILFV-----VKCTSEDE----DGRRRIKGLD 180

Query: 181 EALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQI 240
           E+L +L+ + I  +  K +L  N  + + +LT +L    + S+AY+++LL ++ E     
Sbjct: 181 ESLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSH 240

Query: 241 QILSLNTDFFVEIVQILKDQIS-----------------------------------KQA 300
            +  LN + F  I+  LKD ++                                   KQA
Sbjct: 241 IVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQA 300

Query: 301 SKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSSEKRSCEMILVTLDLLCGCAD 360
             +AL +L+   S SRNR   V  GAV  +I+L +   + EKR  E++L  L  LC CA+
Sbjct: 301 VTAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCAN 360

Query: 361 GRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLC 418
           GRAE+L H  GIAVV+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC
Sbjct: 361 GRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLC 420

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.7e-50
Identity = 152/424 (35.85%), Postives = 227/424 (53.54%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCPVT+  LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEA 183
            LRRLR  A  S+ N+  + A  A E L  I+ + T    SSE            L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---SSE------------LVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR---AYSVMLLTSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R   A  + ++++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  ++ID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.0e-47
Identity = 147/424 (34.67%), Postives = 222/424 (52.36%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  + TCPVT+V LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIV-CNSTNVEFSSEDYAYLIDDQFANLGDE 183
            +RRLR +A  SE N+  +    A E L  I+  +      SSE            L  E
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSE------------LVSE 187

Query: 184 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 243
           +L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  + 
Sbjct: 188 SLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 247

Query: 244 ---ILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMID 303
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  ++ID
Sbjct: 248 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 307

Query: 304 -LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAV 363
            L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSS 416
           G L  +A  +A E    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

[Close]

BLAST of Cp4.1LG10g07220 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 817 bits (2110), Expect = 4.83e-298
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180
           RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI
Sbjct: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 423
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of Cp4.1LG10g07220 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 803 bits (2074), Expect = 1.48e-292
Identity = 412/423 (97.40%), Postives = 420/423 (99.29%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSDRHKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPNHT+RRVIQAWCT+NACKGIERIPTPKPP+DCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180
           RLRSIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQFANLGDEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PV+IDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 423
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of Cp4.1LG10g07220 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 796 bits (2057), Expect = 5.77e-290
Identity = 413/423 (97.64%), Postives = 417/423 (98.58%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDR+SIEKWLFSD HKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60

Query: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPN TLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180
           RLRSIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQ ANLGDEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           L+KLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPV+IDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 423
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of Cp4.1LG10g07220 vs. NCBI nr
Match: XP_022950790.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 794 bits (2051), Expect = 4.74e-289
Identity = 409/423 (96.69%), Postives = 416/423 (98.35%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSDRHKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           +FDITPNHTLRRVIQAWCTVNACKG+ERIPTPKPPVDCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180
           RL+SIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQ ANLG EALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPV+IDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 423
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of Cp4.1LG10g07220 vs. NCBI nr
Match: KAG6603504.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 750 bits (1937), Expect = 4.86e-272
Identity = 389/401 (97.01%), Postives = 394/401 (98.25%), Query Frame = 0


Query: 23  MKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFDITPNHTLRRVIQAWCTVNA 82
           MKDPVTVASGITYDRESIEKWLFSD HKTCPVTQVVLSSFDITPN TLRRVIQAWCTVNA
Sbjct: 1   MKDPVTVASGITYDRESIEKWLFSDGHKTCPVTQVVLSSFDITPNITLRRVIQAWCTVNA 60

Query: 83  CKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIAAGSESNKRCMEAAGAV 142
           CKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIAA SESNKRCMEAAGAV
Sbjct: 61  CKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIAAASESNKRCMEAAGAV 120

Query: 143 EFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSILYKLQISESGLKFLLANNGGIF 202
           EFLAGIVCNSTNVEFS EDYAYLIDDQ ANLGDEALSILYKLQISESGLKFLLANNGGIF
Sbjct: 121 EFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSILYKLQISESGLKFLLANNGGIF 180

Query: 203 VSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFVEIVQILKDQISKQASK 262
           V+TLTKILQNGTYGSRAYSVMLLTSMFEVADQIQI+SLNTD F+EIVQILKDQISKQASK
Sbjct: 181 VTTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIVSLNTDIFIEIVQILKDQISKQASK 240

Query: 263 SALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSSEKRSCEMILVTLDLLCGCADGR 322
           SALKLLIGLCSSSRNRVKAVKAGAVPV+IDLLLDLDSSEKRSCEMILVTLDLLCGCADGR
Sbjct: 241 SALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSSEKRSCEMILVTLDLLCGCADGR 300

Query: 323 AEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL 382
           AEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL
Sbjct: 301 AEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLCFL 360

Query: 383 LQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH 423
           LQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH
Sbjct: 361 LQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFPH 401

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 803 bits (2074), Expect = 7.17e-293
Identity = 412/423 (97.40%), Postives = 420/423 (99.29%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSDRHKTCPVTQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           SFDITPNHT+RRVIQAWCT+NACKGIERIPTPKPP+DCKQVVRILADAKLSPFSQQNCLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180
           RLRSIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQFANLGDEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300
           NTDFF+EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAV+AGA+PV+IDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLC CADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 423
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 794 bits (2051), Expect = 2.29e-289
Identity = 409/423 (96.69%), Postives = 416/423 (98.35%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSDRHKTCP+TQVVLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120
           +FDITPNHTLRRVIQAWCTVNACKG+ERIPTPKPPVDCKQVVRILADAKLS FSQQNCLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180
           RL+SIAA SESNKRCMEAAGAVEFLAGIVCNSTNVEFS EDYAYLIDDQ ANLG EALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240
           LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR YSVMLLTSMFEVADQIQILSL
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300
           NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPV+IDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360
           EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIA+VSKKILRVSTLGSEKAVGILCSIARF
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420
           SASESVVQEMMRLGVVTKLCFLLQV GAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

Query: 421 FPH 423
           FPH
Sbjct: 421 FPH 423

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy TrEMBL
Match: A0A6J1EEF1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478 PE=4 SV=1)

HSP 1 Score: 685 bits (1767), Expect = 3.12e-246
Identity = 356/416 (85.58%), Postives = 380/416 (91.35%), Query Frame = 0


Query: 7   ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFDITP 66
           E+DPPPFFLCPISLQIMKDPVTVASGITYDRE+IE+WLFSD+H TCPVTQVVLS FDITP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 67  NHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLRSIA 126
           N+TL R+IQAWCTVN    +ERIPTPKPPVD KQVV+ILAD KLSPFSQQNCLRRL SIA
Sbjct: 63  NNTLSRLIQAWCTVN--NRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 122

Query: 127 AGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSILYKLQI 186
           + +E NKRCMEAAGAVEFL G+VCNS  +E S  D+A+  DD  ANLGDEALSILYKLQI
Sbjct: 123 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAF--DDPLANLGDEALSILYKLQI 182

Query: 187 SESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNTDFFV 246
           SESGLKFLLANNGGIFV+TLTK LQNGTYGSRAY+ MLL SMFEVAD+IQI SLN +FFV
Sbjct: 183 SESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFV 242

Query: 247 EIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSSEKRSCE 306
           EIVQILKDQISKQASKSALKLLIGLCSS RNRV AV+AGAVPV+IDLLLDLDSSEKR CE
Sbjct: 243 EIVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCE 302

Query: 307 MILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESV 366
           MILV LDLLCGCADGRAE+LNHAAGIAVVSKKILRVS+LGSEKAVGILCSIARFSAS+SV
Sbjct: 303 MILVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSV 362

Query: 367 VQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSFP 422
           VQEM RLGVV KLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSC PSSLR S+P
Sbjct: 363 VQEMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 675 bits (1742), Expect = 3.00e-242
Identity = 353/427 (82.67%), Postives = 389/427 (91.10%), Query Frame = 0


Query: 1   MAELD---QELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQV 60
           MAELD   Q +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIEKWLFSD+HKTCPV+ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSSFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQN 120
           VLS FD+TPNHTLRRVIQAWCTVNA KG+ERIPTPKPPVD +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNST-NVEFSSEDYAYLIDDQFANLGDE 180
           CL RLRSIAA +ESNKRCME+AG VEFLAG+VCNST N+E+  EDYA+  DD  AN  DE
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPV+IDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIAVVSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 422
           +LRS++P
Sbjct: 421 TLRSAYP 425

[Close]

BLAST of Cp4.1LG10g07220 vs. ExPASy TrEMBL
Match: A0A1S3B4K2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 SV=1)

HSP 1 Score: 675 bits (1742), Expect = 3.00e-242
Identity = 353/427 (82.67%), Postives = 389/427 (91.10%), Query Frame = 0


Query: 1   MAELD---QELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQV 60
           MAELD   Q +DPPP+FLCPISLQIMKDPVT+ASGITYDRESIEKWLFSD+HKTCPV+ +
Sbjct: 1   MAELDDQDQVVDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHI 60

Query: 61  VLSSFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQN 120
           VLS FD+TPNHTLRRVIQAWCTVNA KG+ERIPTPKPPVD +QVVRILADAKLSPFSQ+N
Sbjct: 61  VLSDFDVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKN 120

Query: 121 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNST-NVEFSSEDYAYLIDDQFANLGDE 180
           CL RLRSIAA +ESNKRCME+AG VEFLAG+VCNST N+E+  EDYA+  DD  AN  DE
Sbjct: 121 CLCRLRSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAF--DDMSANSADE 180

Query: 181 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 240
           AL IL+KLQISES LKFLL+NNGGIF+STLTKILQN +Y SR+YSVMLL SMFEVADQIQ
Sbjct: 181 ALIILHKLQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQ 240

Query: 241 ILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLD 300
           I +L  DFF+EI+QILKDQISKQASKSALKLLI + SS RNRVKAV+AGAVPV+IDLLLD
Sbjct: 241 IQNLTADFFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLD 300

Query: 301 LDSSE-KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILC 360
           LDSSE KR CEMILV LD LCGCADGRAE+LNHAAGIAVVSKKILR+ST+GSEKAVGIL 
Sbjct: 301 LDSSENKRLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILW 360

Query: 361 SIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPS 420
           SIARFS S+SVVQEM+R+GVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLPS
Sbjct: 361 SIARFSGSQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPS 420

Query: 421 SLRSSFP 422
           +LRS++P
Sbjct: 421 TLRSAYP 425

[Close]

BLAST of Cp4.1LG10g07220 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 436.4 bits (1121), Expect = 2.6e-122
Identity = 236/435 (54.25%), Postives = 309/435 (71.03%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           +DQE++ P FFLCPISL IMKDPV V++GITYDRESIEKWLFS +  +CPVT+ V++  D
Sbjct: 1   MDQEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD 60

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHTLRR+IQ+WCT+NA  GIERIPTPKPP+   ++ +++ ++  S  +Q  CL+RLR
Sbjct: 61  LTPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLR 120

Query: 124 SIAAGSESNKRCMEAAGAVEFLAGIVCNST---NVEFSSEDYAYLID-----------DQ 183
            I + + +NKRC+EAA   EFLA IV NS    N   SS   + L D           D 
Sbjct: 121 QIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDS 180

Query: 184 FANLGDEALSILYKLQISESGLKFLLANNGGI-FVSTLTKILQNGTYGSRAYSVMLLTSM 243
             +L DEALS+LY L  SE+ LK LL N  G   V TLTKI+Q G Y SRAY+ +LL  +
Sbjct: 181 SRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 240

Query: 244 FEVADQIQILSLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVP 303
            EVAD +QI+ L  + F E++QIL DQIS +A++SA+++L+  C   RNR KAV+ G + 
Sbjct: 241 LEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTIS 300

Query: 304 VMIDLLL-DLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGS 363
           ++I+LL+ D  SSE+R+ EM +V LD+LC CA+GRAE LNH A IAVVSKKILRVS + S
Sbjct: 301 MIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITS 360

Query: 364 EKAVGILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTW 423
           E+AV +L S+ RF A+ S++QEM++LGVV KLC +LQ V  G K KEKAKE+LKLH R W
Sbjct: 361 ERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVW 420

[Close]

BLAST of Cp4.1LG10g07220 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 423.7 bits (1088), Expect = 1.7e-118
Identity = 219/421 (52.02%), Postives = 305/421 (72.45%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           +D+E++ PPFFLCPISL+IMKDPV V++GITYDR+SIEKWLF+ +  +CPVT+  ++  D
Sbjct: 6   MDEEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDAD 65

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLRRLR 123
           +TPNHTLRR+IQ+WCT+NA  G+ERIPTP+PP+   ++ +++ D+  S  +Q  CL+RLR
Sbjct: 66  LTPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLR 125

Query: 124 SIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSILYK 183
            I + + +NKRC+EAAG  EFLA IV N               D +  +L DEAL++LY 
Sbjct: 126 QIVSENATNKRCLEAAGVPEFLANIVSN---------------DSENGSLTDEALNLLYH 185

Query: 184 LQISESGLKFLLAN-NGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSLNT 243
           L+ SE+ LK LL N      V +LTKI+Q G Y SR Y+ +LL ++ EVAD +Q ++L  
Sbjct: 186 LETSETVLKNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP 245

Query: 244 DFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLD-SSE 303
           + F E+VQIL D+IS++A+K+A+ +L+ +C   RNR KAV+AG + V+I+LL+D   +SE
Sbjct: 246 EVFTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSE 305

Query: 304 KRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFS 363
           +R  EM +V LDLLC CA+GRAE LNH A IAVV KKILRVS   S++AV +L S+ RF 
Sbjct: 306 RRGPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFC 365

Query: 364 ASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSSF 423
           A+ +++ EM++LGVV KLC +LQ V  GGK KEKAKE+LKLH R W++S CLP ++  ++
Sbjct: 366 ATPALLHEMLQLGVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAY 410

[Close]

BLAST of Cp4.1LG10g07220 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 233.4 bits (594), Expect = 3.3e-61
Identity = 165/457 (36.11%), Postives = 248/457 (54.27%), Query Frame = 0


Query: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60
           M + ++E++ P +F+CPISL+IMKDPVT  SGITYDR++I KWL  ++  +CPVT+  L 
Sbjct: 15  MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 74

Query: 61  -SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK---LSPFSQQ 120
              D+TPNH LRR+IQ WC  N  +G+ RI TP+ P     VV  + + K        ++
Sbjct: 75  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 134

Query: 121 NCLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLG-D 180
             L++L  +A    +N+R M   G  + L   V     V+ +SED     D +    G D
Sbjct: 135 ETLQKLEVLAMDG-NNRRLMCECGVHKSLILFV-----VKCTSEDE----DGRRRIKGLD 194

Query: 181 EALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQI 240
           E+L +L+ + I  +  K +L  N  + + +LT +L    + S+AY+++LL ++ E     
Sbjct: 195 ESLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSH 254

Query: 241 QILSLNTDFFVEIVQILKDQIS-----------------------------------KQA 300
            +  LN + F  I+  LKD ++                                   KQA
Sbjct: 255 IVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQA 314

Query: 301 SKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSSEKRSCEMILVTLDLLCGCAD 360
             +AL +L+   S SRNR   V  GAV  +I+L +   + EKR  E++L  L  LC CA+
Sbjct: 315 VTAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCAN 374

Query: 361 GRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARFSASESVVQEMMRLGVVTKLC 418
           GRAE+L H  GIAVV+K++LRVS    ++A+ IL ++++FS    VV+EM+ +G V KLC
Sbjct: 375 GRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLC 434

[Close]

BLAST of Cp4.1LG10g07220 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 199.9 bits (507), Expect = 4.1e-51
Identity = 152/424 (35.85%), Postives = 227/424 (53.54%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL++M+DPVTV +G TYDR SIE W+    + TCPVT+  LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEA 183
            LRRLR  A  S+ N+  + A  A E L  I+ + T    SSE            L  E+
Sbjct: 128 ALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETT---SSE------------LVSES 187

Query: 184 LSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSR---AYSVMLLTSMFEVADQ 243
           L++L  L I+E      ++++ G  V  LT++L + +  +R   A  + ++++  + AD 
Sbjct: 188 LALLVMLPITEPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADL 247

Query: 244 IQILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMID- 303
              +S +   F  ++ +L++ I S++A K  +K L  LCS    R  A+ AGA  ++ID 
Sbjct: 248 KGSISNSESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDR 307

Query: 304 LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVG 363
           L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A G
Sbjct: 308 LAADFDRCD---TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG 367

Query: 364 ILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSC 417
            L  +A  +A E   +E    GVV +L  ++Q      +AK+KA+++LKL   +W + + 
Sbjct: 368 AL--LALCTAEERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSWPDYNS 409

[Close]

BLAST of Cp4.1LG10g07220 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 191.4 bits (485), Expect = 1.4e-48
Identity = 147/424 (34.67%), Postives = 222/424 (52.36%), Query Frame = 0


Query: 4   LDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLSSFD 63
           LD  +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  + TCPVT+V LS F 
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFT 67

Query: 64  ITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAK------LSPFSQQN 123
           + PNHTLRR+IQ WC  N   G+ERIPTPK P D   V  +L+ A       +S  S+  
Sbjct: 68  LIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAA 127

Query: 124 CLRRLRSIAAGSESNKRCMEAAGAVEFLAGIV-CNSTNVEFSSEDYAYLIDDQFANLGDE 183
            +RRLR +A  SE N+  +    A E L  I+  +      SSE            L  E
Sbjct: 128 AIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSE------------LVSE 187

Query: 184 ALSILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQ 243
           +L++L  L ++E+  + + ++     V  +T++L + +   R  +  L+  +   A  + 
Sbjct: 188 SLALLVLLHMTETECEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 247

Query: 244 ---ILSLNTDFFVEIVQILKDQI-SKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMID 303
              I+S +   F  ++ +LK+ I S++A K  +K +  LC   + R  A+ AGA  ++ID
Sbjct: 248 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 307

Query: 304 -LLLDLDSSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAV 363
            L  D D  +    E  L T++LLC   +G A    HA  + ++ K ILRVS   +E A 
Sbjct: 308 RLAADFDRCD---TERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAA 367

Query: 364 GILCSIARFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSS 416
           G L  +A  +A E    E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 368 GAL--LALCTAEERCRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 410

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g07220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVC6	3.6e-121	54.25	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q84TG3	2.4e-117	52.02	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SF15	4.7e-60	36.11	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	5.7e-50	35.85	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	2.0e-47	34.67	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of Cp4.1LG10g07220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023543358.1	4.83e-298	100.00	E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo]	[more]
XP_022978192.1	1.48e-292	97.40	E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima]	[more]
KAG7033684.1	5.77e-290	97.64	E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022950790.1	4.74e-289	96.69	E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata]	[more]
KAG6603504.1	4.86e-272	97.01	E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cp4.1LG10g07220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IKG7	7.17e-293	97.40	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P...	[more]
A0A6J1GGQ8	2.29e-289	96.69	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790...	[more]
A0A6J1EEF1	3.12e-246	85.58	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478...	[more]
A0A5A7UVZ1	3.00e-242	82.67	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B4K2	3.00e-242	82.67	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485961 PE=4 ...	[more]

BLAST of Cp4.1LG10g07220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52450.1	2.6e-122	54.25	plant U-box 22	[more]
AT2G35930.1	1.7e-118	52.02	plant U-box 23	[more]
AT3G11840.1	3.3e-61	36.11	plant U-box 24	[more]
AT3G19380.1	4.1e-51	35.85	plant U-box 25	[more]
AT1G49780.1	1.4e-48	34.67	plant U-box 26	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 13..77 e-value: 3.5E-34 score: 129.6
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 11..79 e-value: 7.1E-21 score: 74.2
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 9..84 score: 42.098454
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..86 e-value: 2.0E-28 score: 100.2
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 98..404 e-value: 6.7E-17 score: 63.3
None	No IPR available	PANTHER	PTHR22849:SF132	E3 UBIQUITIN-PROTEIN LIGASE PUB23	coord: 7..422
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 7..422
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 14..56 e-value: 3.78377E-22 score: 86.5186
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 7..85
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 102..384

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG10g07220.1	Cp4.1LG10g07220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity