Cp4.1LG09g11480 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g11480
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Peptidyl-tRNA hydrolase
Location	Cp4.1LG09: 9774423 .. 9778728 (+)
RNA-Seq Expression	Cp4.1LG09g11480
Synteny	Cp4.1LG09g11480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAATTTTGGTTCCTCCCGCCAATCCTCAATCAATCCAATCTCTCTATCAAACCAAAATCACAGCCTCTTGCCTTGCCATCGTCTCCTCCTCCCGCCATTGTTATATCTTCTTCACTTCACTCCTCTCCAATTCTTCCCCCTTTTCAAATCATTCAAGAAACGCCCCACCAGATATGAGTTTTGGTGCTTCTCTATCTTCAGTCGCCGCCCTGCGCTTTCCTATTTGTCTCCATTGTTTTCGAACCCCCAGAACACCCATTTCCTTCCCTTATCTATCCTTAAATCCTGGAAGTTTGCATATCTTTGCGTCGATGACTTCTTCCTTTACTGCGTCGATGTCTACTTCCTCACCTCTGGCCCCCGATCAGCTTGCTGTGGAGGAGAAGAAGCCCGCGCCGCCGCCGAAGCCATGGCTGATCGTTGGCCTTGGGAATCCCGGGAAGAAGTATGAACGCACGCGCCATAATGTCCGTATGGTCCTATCTTTTCGTTTACGAATTGAACTTGCTCTTTTTTGTACCATTTATTCTTTGTTCCTTTTATATCGAGCGCGAAGGATTATTCATTTATAGTATTTAGGTAGGAAATTAAATTCTGTTTCATTGAGAACTATGTTGATCTAGACGATTATGCCTTATTTTCTGTTGGGTTAACTGGGTATGCCGTTTATCGTGTGGGAATCCCCTTAGGATAGGTTTTCGAGCATTAAATTTGGTTATCTCGAGGAAATTATGATTTATGTTGATTTACAAAGCTGCAGAACGATGTCTTATTTAGTGCTGTTTTTGTTATTAGCCATATTGAGTGTTGTCTGGATGCCCTTTATGAAGACGAATTGAAGCATGGCAGTCCCTGATGCTATGATTAATTTTCCAGGTAGGTTTTGAGATGGTGGATGCTGTCGCTGAGGTTGAAGGGATATCCATGAGTAGTGTTTCCTTCAAAGCTCTTATCGGGAAAGGTGTGCAGATTTTAACAAACTCGTTGTTCTTACCTACTGATTTTTGACAATTCTCGACAGTTGATAAAATTAAAATATCTGGCTAGAACTTAGAAGTACATCTAGCTGCTAGTTTTCTTGCTTTGAACGGACTTGCAAGTAGATTACTGACTCCTTTCTTCCCTGTTTGAAGATGTGTAAACTTTTCATCCTTATCAATTAGATTATCTGTTATCAGACTCATATTGACTTCTAAATTCTATTTGTGCATTCTCAAACCATAATAAGCAATTTTTGCTGGTTCGAGTTTGTTTGACAAGATACTCTTTTTCTTTTAGTTCAACAATTGGAGGATGGAGGGATTGACCATAGACCTCAGGATAGTAATTATTGTTTTATCCCTCAGTTTGCATGCACCTCAACTATTTTTATGTATCAACCGTGTAAGCCTACTATTTTTTATGTTTGTGATGGAGCCCAAATATATTGAAATGTAAAAGATAATGGACTTACTATATACTTCTCAAGTATTTGAGGTACTTCCACCACTCTTGTGATACCACTCAAGCTTGAAGTCACTAAACAATTGCATGCCTTCATATCTCCCCTCTAACTCTATTTATAACCATGTCCACTGACAACTTCTTACCTAATTACTAATACTCCCTTATATTTCCATTTCTGTCCTAATTGCATTCATATTAATTTGGATGCCAAGGAAACTCCAAGCATATTATTGAACTCATTCTCTCTTGGCTAGTACGTGGTTTTTATTAATGTTTGAATTCTTTTATGAAGATGAAATATCAAGAAAAACCAATTTTTCTTGCCCCCAAAAAAGGAGTTCTTGTCTAGGAAAAATTATTTAGTATATGATTATTTCCAGCTGGAGATTGCTACAATAGTGAAGATATTCTATCAATGTTTACTTGTATTTTAGGTTTCATCGGGGATGTTCCTATAATGCTTGCCAAGCCACAAACTTTCATGAATGCAAGTGGTGAGTCTGTAAGTAAAGCAATCTCAAGGATCTCCATCCATTTTAATTATTAAGTTTACCCTTTGGTCTCAATGATTGATTTTTTATGCATTTAACCTTTGATTTCAGGTTGGTGCTATTGTTTCCTACTATAAAATTCCATTGAAGCAAGTCCTTGTGGTAAATATTTTATTCCCCATGTGAGATTATCAGTTTTCTTGACATGAGAGGAGGTTGGGCTGTGGGGTGGGTATCTATGTTATTCACTTTCTTTTTCCCCTACATCTAAGCCTTGAATTGGGGTCCTTCAACAGATATTCGATGACTTAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGGTAATTGTCTTTTTTGTATTATAATTCAGCTTAACGCTTCATTTTGGGTGTCTCAGCCTTCTGAGTTTTGTGGCATTCATTTTCTAATATCCTTAATACTAGGAGGTATAGTGGTAGGTTTTCATATATTTTCTTCGGGGTCATGCTGCATAATATCCCCTGCCTTTTTATTTTTATTAACAGAAGTTGCATACAGTGAGCTTCTAGCCCTTTGTATCCGAACAATGCCATTTTTTATTTACTTATTTTCTTTATTCTGTTTTGACAATCCTGCGTGTGTTGTCCATCAGAAGAAAGAGAAGCATATTCCATTTTAAGGGATTTCTGGTTGAAAGATTGTGTTTTTGCAGAATTTTGTCCCTCAAGGAAAAGTCTACTTTCTCTAAGTATGATCTTTTACTTGAAAAGTTTTGACCGCAAAAGTTTTCCTCCCTATAGAAGAGCATGCTTTCTTCACATCTGAATATAGATTTTTACTAGAGTTTTTTGTAGCTTTTAAGAGGAAAAAAAATTGAGTTGACCAAGTAATATTAAATGTAAAAGTTCATTAAATATAACGTGTGCACCTGATCAAATAATTTAAGCTCGGTACAAGGGTCTAGGGGAATACGAGTATTTATTGAATGATTAAGATGTAATAGAATAAACATTAAGAAGAGCAAAAGGCATAGAAGTGCAACTTTTAATTTGTTTATTTGGTTTGTGTGGCAGGATGAGGAGTATTATTGATCACTTGAAAGGCAGTCGTGATTTCCCTCGGTTAAGAATAGGTACGTCCCTTGAGGCTTTCGAACTGCTTCTATATAATATCAGCTGTTAAATGAGTGAAAACATTCCAGGCATTGGGCGCCCTCCCGGGAAAATGGATGCTGCGAACTTTGTTATGCGCCCCTTCAACAAACAAGAACGAGAAGAGGTGAGTAAAATGGCATCACATTCCACAAAATAAAGTTTGTTGATAATGGAACTCAGTTTTTAACCATCCGAGTGGAATCTCTTTTCAGTTGGATTTTACAATGCAAGTCGGAGTAGACGCAATACGGATACTTTTGCTGGAGGGGTTTAACAAAAGTGCAACATTTGTCAACACCAACAAACCTCTGGAACAAATAGGCTGAAAACATTCAGTGTTTAACTTTAAACCTTTTTATGGACTCTAATAGGGCTCCAGCCATTTTGTTTACTGTATCAAACTGCTTTAGAAACTGCTTTATACACAGGCGGTATTTGCTACCAGTTTCAACTCTATAGCAACATCCTTGAAAGCTTTGGATAGGATTATGGGGGAGGAAGACATGACAATCAGTGTAATTTTTGAACCCCATCAATGTAAGCACATTGTCTTTCTTATTGAAGCATCTCTCTCTGTCTCTTGGAATTGAAATATGTTAAGACATAAAGTAGTGATCTTTGAAGAGATCAATGAAGCCAATAACCTCCCCCACCATGCAGCAAAGAGACCGATAAAAGTTGAGAAGGACAGGAATTGATAGAACGGAGCAAGCTGCCTCTACGGCTCTACCTAACTTTGAATGCTTTAATTATCTCATCTTGACATCACCGAGGGAGGCTATTCTTTGCCTGTCTTTGTCATCTTCTTCTCCTTTATGCATTTTCTCTGTAGTTTAACCATTCGCGTCCTCTATTCTTCATGTTTGTCTTGCCTGTGTTTTCAGGTTTCTGGTGAATGCCTTCCCTATGGAATTGCCTCATACGTGGATGATTTCATGCAGGTGGATCCCACATAATTTTGAATGTACGTCATATGCTTCAAAAACTAATTATTTCATGGTATCTGTGTTTATTTATTTATTTTAAAATTGATTCCTACTATTTTAGAAGTTTTTATTTAGTATTTATGGTTAAGCGTGGCCATATGGTTAGGACCAAATTGGCTTGAAGATTTAAAATGTTGATGTCAACCAAAATATTGATATTATGATGATGTTGATGGATATTTCAGTATTACACTTGAAGAACTGTTAAGAGTTGTGTAT

mRNA sequence

ATCAATTTTGGTTCCTCCCGCCAATCCTCAATCAATCCAATCTCTCTATCAAACCAAAATCACAGCCTCTTGCCTTGCCATCGTCTCCTCCTCCCGCCATTGTTATATCTTCTTCACTTCACTCCTCTCCAATTCTTCCCCCTTTTCAAATCATTCAAGAAACGCCCCACCAGATATGAGTTTTGGTGCTTCTCTATCTTCAGTCGCCGCCCTGCGCTTTCCTATTTGTCTCCATTGTTTTCGAACCCCCAGAACACCCATTTCCTTCCCTTATCTATCCTTAAATCCTGGAAGTTTGCATATCTTTGCGTCGATGACTTCTTCCTTTACTGCGTCGATGTCTACTTCCTCACCTCTGGCCCCCGATCAGCTTGCTGTGGAGGAGAAGAAGCCCGCGCCGCCGCCGAAGCCATGGCTGATCGTTGGCCTTGGGAATCCCGGGAAGAAGTATGAACGCACGCGCCATAATGTAGGTTTTGAGATGGTGGATGCTGTCGCTGAGGTTGAAGGGATATCCATGAGTAGTGTTTCCTTCAAAGCTCTTATCGGGAAAGGTTTCATCGGGGATGTTCCTATAATGCTTGCCAAGCCACAAACTTTCATGAATGCAAGTGGTGAGTCTGTTGGTGCTATTGTTTCCTACTATAAAATTCCATTGAAGCAAGTCCTTGTGATATTCGATGACTTAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGGATGAGGAGTATTATTGATCACTTGAAAGGCAGTCGTGATTTCCCTCGGTTAAGAATAGGCATTGGGCGCCCTCCCGGGAAAATGGATGCTGCGAACTTTGTTATGCGCCCCTTCAACAAACAAGAACGAGAAGAGTTGGATTTTACAATGCAAGTCGGAGTAGACGCAATACGGATACTTTTGCTGGAGGGGTTTAACAAAAGTGCAACATTTGTCAACACCAACAAACCTCTGGAACAAATAGGCTGAAAACATTCAGTGTTTAACTTTAAACCTTTTTATGGACTCTAATAGGGCTCCAGCCATTTTGTTTACTGTATCAAACTGCTTTAGAAACTGCTTTATACACAGGCGGTATTTGCTACCAGTTTCAACTCTATAGCAACATCCTTGAAAGCTTTGGATAGGATTATGGGGGAGGAAGACATGACAATCAGTGTAATTTTTGAACCCCATCAATCAAAGAGACCGATAAAAGTTGAGAAGGACAGGAATTGATAGAACGGAGCAAGCTGCCTCTACGGCTCTACCTAACTTTGAATGCTTTAATTATCTCATCTTGACATCACCGAGGGAGGCTATTCTTTGCCTGTCTTTGTCATCTTCTTCTCCTTTATGCATTTTCTCTGTAGTTTAACCATTCGCGTCCTCTATTCTTCATGTTTGTCTTGCCTGTGTTTTCAGGTTTCTGGTGAATGCCTTCCCTATGGAATTGCCTCATACGTGGATGATTTCATGCAGGTGGATCCCACATAATTTTGAATGTACGTCATATGCTTCAAAAACTAATTATTTCATGGTATCTGTGTTTATTTATTTATTTTAAAATTGATTCCTACTATTTTAGAAGTTTTTATTTAGTATTTATGGTTAAGCGTGGCCATATGGTTAGGACCAAATTGGCTTGAAGATTTAAAATGTTGATGTCAACCAAAATATTGATATTATGATGATGTTGATGGATATTTCAGTATTACACTTGAAGAACTGTTAAGAGTTGTGTAT

Coding sequence (CDS)

ATGAGTTTTGGTGCTTCTCTATCTTCAGTCGCCGCCCTGCGCTTTCCTATTTGTCTCCATTGTTTTCGAACCCCCAGAACACCCATTTCCTTCCCTTATCTATCCTTAAATCCTGGAAGTTTGCATATCTTTGCGTCGATGACTTCTTCCTTTACTGCGTCGATGTCTACTTCCTCACCTCTGGCCCCCGATCAGCTTGCTGTGGAGGAGAAGAAGCCCGCGCCGCCGCCGAAGCCATGGCTGATCGTTGGCCTTGGGAATCCCGGGAAGAAGTATGAACGCACGCGCCATAATGTAGGTTTTGAGATGGTGGATGCTGTCGCTGAGGTTGAAGGGATATCCATGAGTAGTGTTTCCTTCAAAGCTCTTATCGGGAAAGGTTTCATCGGGGATGTTCCTATAATGCTTGCCAAGCCACAAACTTTCATGAATGCAAGTGGTGAGTCTGTTGGTGCTATTGTTTCCTACTATAAAATTCCATTGAAGCAAGTCCTTGTGATATTCGATGACTTAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGGATGAGGAGTATTATTGATCACTTGAAAGGCAGTCGTGATTTCCCTCGGTTAAGAATAGGCATTGGGCGCCCTCCCGGGAAAATGGATGCTGCGAACTTTGTTATGCGCCCCTTCAACAAACAAGAACGAGAAGAGTTGGATTTTACAATGCAAGTCGGAGTAGACGCAATACGGATACTTTTGCTGGAGGGGTTTAACAAAAGTGCAACATTTGTCAACACCAACAAACCTCTGGAACAAATAGGCTGA

Protein sequence

MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSPLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG

Homology

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BLAST of Cp4.1LG09g11480 vs. ExPASy Swiss-Prot
Match: Q8GW64 (Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g18440 PE=2 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 2.9e-95
Identity = 183/280 (65.36%), Postives = 215/280 (76.79%), Query Frame = 0


Query: 8   SSVAALRFP--ICLHCFRTPRTPIS--FPYLSLNPGSLHI---------FASMTSSFTAS 67
           + +A L FP   C   FR+P T +S   P   L  G   +          +S + SF+++
Sbjct: 8   AKIANLSFPSNCCSLFFRSPATFLSPALPCRKLTKGIRGLEGLMSQCLSSSSQSLSFSSN 67

Query: 68  MSTSSPLAPDQLAVEEKKPAPPP--KPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEG 127
             +S P +    A+   KP  PP   PWLIVGLGNPGKKY+ TRHNVGFEMVDA+A+ EG
Sbjct: 68  SFSSQPESELLQALPSSKPKSPPLQLPWLIVGLGNPGKKYQGTRHNVGFEMVDALADAEG 127

Query: 128 ISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLD 187
           ISM++V+FKAL GKG IG++PIMLAKPQTFMN SGESVG IVS+YKIPLKQVLV++DDLD
Sbjct: 128 ISMNTVNFKALFGKGVIGNIPIMLAKPQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLD 187

Query: 188 LPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQ 247
           LPF KLR+LPKGGHGGHNGMRSIID LKGSRDFPRLRIGIGRPPGKMD ANFV+R FN+Q
Sbjct: 188 LPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQ 247

Query: 248 EREELDFTMQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 273
           E+EELD T Q G++AIRILLLEGFNKSATFVNT K +EQ+
Sbjct: 248 EQEELDHTFQTGLEAIRILLLEGFNKSATFVNTRKSMEQL 287

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy Swiss-Prot
Match: Q5N9Q7 (Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0693900 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 3.8e-87
Identity = 148/195 (75.90%), Postives = 174/195 (89.23%), Query Frame = 0


Query: 78  KPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLA 137
           KPWL VGLGNPGK Y+ TRHNVGFEM+D +AE EGIS+SS+ FKA++GKG IGD PIMLA
Sbjct: 54  KPWLFVGLGNPGKVYQGTRHNVGFEMIDVIAEAEGISLSSMQFKAMVGKGRIGDAPIMLA 113

Query: 138 KPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIID 197
           KPQTFMNASGESVG +VSY+KIPL QVLV++DDLDLPFAKLR+LPKGGHGGHNG+RSII+
Sbjct: 114 KPQTFMNASGESVGQLVSYFKIPLNQVLVMYDDLDLPFAKLRLLPKGGHGGHNGVRSIIN 173

Query: 198 HLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFN 257
           HLK +RDFPRLRIGIGRPPGKMD ANFV+RPFN++E+EELDF    G++A+RI+ LEGFN
Sbjct: 174 HLKQNRDFPRLRIGIGRPPGKMDPANFVLRPFNRKEQEELDFAFHRGLEAVRIMALEGFN 233

Query: 258 KSATFVNTNKPLEQI 273
           KSAT+VNT +  E +
Sbjct: 234 KSATYVNTAQSSEML 248

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy Swiss-Prot
Match: Q6NLS8 (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 6.7e-60
Identity = 99/195 (50.77%), Postives = 146/195 (74.87%), Query Frame = 0


Query: 78  KPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLA 137
           +PWL +GLGNPG KY+ TRHN+GFEM+D  AE  GI M+ V+FKA++G+GF+ D+P++LA
Sbjct: 17  RPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVILA 76

Query: 138 KPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIID 197
           KPQT+MN SGES G + +YYK+PL +VLV+ DD+ LP   LR+  KGGHG HNG++S+++
Sbjct: 77  KPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMN 136

Query: 198 HLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFN 257
           H +G+R+F RLRIGIG+PPG+MD   F+++ F+   RE +D  +  GVDA++++L + F 
Sbjct: 137 HFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFG 196

Query: 258 KSATFVNTNKPLEQI 273
           +S    N  +  + +
Sbjct: 197 ESWRLFNVEQKYKHL 211

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy Swiss-Prot
Match: Q9M5P4 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays OX=4577 GN=CRS2 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.3e-58
Identity = 108/224 (48.21%), Postives = 153/224 (68.30%), Query Frame = 0


Query: 44  FASMTSSFTASMSTSSPLAPDQLAVEEKKPAPPP---KPWLIVGLGNPGKKYERTRHNVG 103
           F     S TA +       P  +      PA  P    PWLI GLGNPG KY  TRHNVG
Sbjct: 23  FRMPRKSLTAPLHRIRRPRPFTVVSSVPDPAAGPVEYTPWLIAGLGNPGNKYYGTRHNVG 82

Query: 104 FEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIP 163
           FEMVD +A  EGI+M+++  K+L+G G IG+VP+++ KPQ++MN SGE++G + +YY++P
Sbjct: 83  FEMVDRIAAEEGITMNTIQSKSLLGIGSIGEVPVLVVKPQSYMNYSGEAIGPLAAYYQVP 142

Query: 164 LKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMD 223
           L+ +L+I+DD  LP   LR+  KGGHG HNG++++I+HL G R+FPRL IGIG PPGKMD
Sbjct: 143 LRHILLIYDDTSLPNGVLRLQKKGGHGRHNGLQNVIEHLDGRREFPRLSIGIGSPPGKMD 202

Query: 224 AANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFNKSATFVN 265
              F+++ F+ +ER ++D  ++ GVDA+R L+L+GF+ S    N
Sbjct: 203 PRAFLLQKFSSEERVQIDTALEQGVDAVRTLVLKGFSGSTERFN 246

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy Swiss-Prot
Match: Q9LF14 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.7e-58
Identity = 100/186 (53.76%), Postives = 143/186 (76.88%), Query Frame = 0


Query: 79  PWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLAK 138
           PWLIVGLGNPG KY  TRHNVGFEM+D +A  EG+ M+++  KALIG G I DVPI+LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 139 PQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDH 198
           PQT+MN SGESVG++ S+Y++PL+ +L+I+D++ LP   LR+ PKGG G HNG++S++ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 199 LKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFNK 258
           L G R+FPRL IGIG+PPG MD   F+++ F+  E+++++  ++ G +A++ L+L GFN+
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 259 SATFVN 265
             +  N
Sbjct: 225 GISRFN 230

[Close]

BLAST of Cp4.1LG09g11480 vs. NCBI nr
Match: XP_023541955.1 (peptidyl-tRNA hydrolase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546 bits (1406), Expect = 8.34e-196
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60
           MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP
Sbjct: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60

Query: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120
           LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF
Sbjct: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180
           KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM
Sbjct: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240
           LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273
           MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273

[Close]

BLAST of Cp4.1LG09g11480 vs. NCBI nr
Match: XP_022994507.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 516 bits (1330), Expect = 3.22e-184
Identity = 259/273 (94.87%), Postives = 264/273 (96.70%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60
           M+  ASLSS+AALR PICLHCF T RTPISFP LSLNP SLHIF+SMTSS TASMSTSSP
Sbjct: 1   MNIAASLSSLAALRLPICLHCFPTFRTPISFPSLSLNPPSLHIFSSMTSSITASMSTSSP 60

Query: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120
           LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEM+DAVAEVEGISMSS SF
Sbjct: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMLDAVAEVEGISMSSASF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180
           KALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM
Sbjct: 121 KALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240
           LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273
           MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273

[Close]

BLAST of Cp4.1LG09g11480 vs. NCBI nr
Match: XP_022954858.1 (peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 515 bits (1326), Expect = 1.31e-183
Identity = 258/273 (94.51%), Postives = 263/273 (96.34%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60
           M+  ASLSS+AALR P+CLHCFRT RTPISFP LSLNP SLHIF+SMTSSFTASMSTSSP
Sbjct: 1   MNIAASLSSLAALRLPVCLHCFRTFRTPISFPSLSLNPPSLHIFSSMTSSFTASMSTSSP 60

Query: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120
           LAPD LAVEE KPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVA VEGISMSS SF
Sbjct: 61  LAPDLLAVEENKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAVVEGISMSSASF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180
           KALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM
Sbjct: 121 KALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240
           LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273
           MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273

[Close]

BLAST of Cp4.1LG09g11480 vs. NCBI nr
Match: KAG6572934.1 (Peptidyl-tRNA hydrolase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514 bits (1324), Expect = 2.64e-183
Identity = 257/273 (94.14%), Postives = 263/273 (96.34%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60
           M+  ASLSS+AALR P+CLHCFRT RTPISFP LSLNP SLHIF+SMTSS TASMSTSSP
Sbjct: 1   MNIAASLSSLAALRLPVCLHCFRTFRTPISFPSLSLNPPSLHIFSSMTSSITASMSTSSP 60

Query: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120
           LAPD LA+EE KPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSS SF
Sbjct: 61  LAPDLLALEENKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSASF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180
           KALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM
Sbjct: 121 KALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240
           LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273
           MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273

[Close]

BLAST of Cp4.1LG09g11480 vs. NCBI nr
Match: XP_038893864.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893865.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893866.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 478 bits (1230), Expect = 1.29e-168
Identity = 242/274 (88.32%), Postives = 255/274 (93.07%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLS-LNPGSLHIFASMTSS-FTASMSTS 60
           M+ G+S+SSV ALR PICLHCFRT +T ISFP LS LN G+ HIF+SMTSS  TASMSTS
Sbjct: 1   MNVGSSISSVTALRLPICLHCFRTSKTLISFPSLSFLNSGTSHIFSSMTSSSITASMSTS 60

Query: 61  SPLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSV 120
           S    DQL V++KKPAPPPKPWLIVGLGNPGKKY+RTRHNVGFEMVD VAE EGISMSSV
Sbjct: 61  SSPTTDQLPVDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDTVAEAEGISMSSV 120

Query: 121 SFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKL 180
           SFKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKL
Sbjct: 121 SFKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKL 180

Query: 181 RMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELD 240
           RMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFV+RPFNKQEREEL+
Sbjct: 181 RMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELN 240

Query: 241 FTMQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 272
           FT+QVG DAIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 FTLQVGTDAIRILLLEGFNKSATFVNTNKPLEQI 274

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy TrEMBL
Match: A0A6J1JW15 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=3 SV=1)

HSP 1 Score: 516 bits (1330), Expect = 1.56e-184
Identity = 259/273 (94.87%), Postives = 264/273 (96.70%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60
           M+  ASLSS+AALR PICLHCF T RTPISFP LSLNP SLHIF+SMTSS TASMSTSSP
Sbjct: 1   MNIAASLSSLAALRLPICLHCFPTFRTPISFPSLSLNPPSLHIFSSMTSSITASMSTSSP 60

Query: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120
           LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEM+DAVAEVEGISMSS SF
Sbjct: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMLDAVAEVEGISMSSASF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180
           KALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM
Sbjct: 121 KALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240
           LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273
           MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy TrEMBL
Match: A0A6J1GS97 (peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456993 PE=3 SV=1)

HSP 1 Score: 515 bits (1326), Expect = 6.34e-184
Identity = 258/273 (94.51%), Postives = 263/273 (96.34%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSLNPGSLHIFASMTSSFTASMSTSSP 60
           M+  ASLSS+AALR P+CLHCFRT RTPISFP LSLNP SLHIF+SMTSSFTASMSTSSP
Sbjct: 1   MNIAASLSSLAALRLPVCLHCFRTFRTPISFPSLSLNPPSLHIFSSMTSSFTASMSTSSP 60

Query: 61  LAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSF 120
           LAPD LAVEE KPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVA VEGISMSS SF
Sbjct: 61  LAPDLLAVEENKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAVVEGISMSSASF 120

Query: 121 KALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180
           KALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM
Sbjct: 121 KALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRM 180

Query: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240
           LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT
Sbjct: 181 LPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFT 240

Query: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273
           MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG
Sbjct: 241 MQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy TrEMBL
Match: A0A1S3C5R3 (peptidyl-tRNA hydrolase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496915 PE=3 SV=1)

HSP 1 Score: 464 bits (1193), Expect = 1.22e-163
Identity = 234/273 (85.71%), Postives = 251/273 (91.94%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSL-NPGSLHIFASMTSSFTASMSTSS 60
           M+ GAS+SSVAALR P  +H FRT RT ISFP LSL +  + H F+S+TSS TASMSTSS
Sbjct: 1   MNIGASISSVAALRLPFSIHSFRTSRTLISFPSLSLLHSPTFHNFSSITSSITASMSTSS 60

Query: 61  PLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVS 120
               DQ A+++KKPAPPPKPWLIVGLGNPGKKY+RTRHNVGFEMVDAVAE EGIS+S+VS
Sbjct: 61  SPTTDQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDAVAEAEGISISNVS 120

Query: 121 FKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180
           FKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR
Sbjct: 121 FKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180

Query: 181 MLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDF 240
           MLPKGGHGGHNGMRSIIDH KGSRDFPRLRIGIGRPPGKMDAANFV+RPFNKQEREEL+F
Sbjct: 181 MLPKGGHGGHNGMRSIIDHFKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240

Query: 241 TMQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 272
           T+QVG DAIRILLLEGFNKSATFVNTNKPLEQI
Sbjct: 241 TLQVGADAIRILLLEGFNKSATFVNTNKPLEQI 273

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy TrEMBL
Match: A0A0A0LVQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042230 PE=3 SV=1)

HSP 1 Score: 459 bits (1181), Expect = 8.20e-162
Identity = 232/273 (84.98%), Postives = 249/273 (91.21%), Query Frame = 0


Query: 1   MSFGASLSSVAALRFPICLHCFRTPRTPISFPYLSL-NPGSLHIFASMTSSFTASMSTSS 60
           M  GAS+SSVAALR P   HCF T +T ISFP LSL +  + HIF+S+TSS TASMSTSS
Sbjct: 1   MIIGASISSVAALRLPFSFHCFPTSKTLISFPSLSLLHSPTFHIFSSITSSITASMSTSS 60

Query: 61  PLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVS 120
               +Q A+++KKPAPPPKPWLIVGLGNPGKKY+RTRHNVGFEMVDAVAE EGIS+S+VS
Sbjct: 61  SPTTEQPALDDKKPAPPPKPWLIVGLGNPGKKYDRTRHNVGFEMVDAVAEAEGISISNVS 120

Query: 121 FKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180
           FKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR
Sbjct: 121 FKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLR 180

Query: 181 MLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDF 240
           MLPKGGHGGHNGMRSIIDH KGSRDFPRLRIGIGRPPGKMDAANFV+RPFNKQEREEL+F
Sbjct: 181 MLPKGGHGGHNGMRSIIDHFKGSRDFPRLRIGIGRPPGKMDAANFVLRPFNKQEREELNF 240

Query: 241 TMQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 272
           T+QVG DAIRILLLEGFNKSATFVNTNK LEQI
Sbjct: 241 TLQVGEDAIRILLLEGFNKSATFVNTNKSLEQI 273

[Close]

BLAST of Cp4.1LG09g11480 vs. ExPASy TrEMBL
Match: A0A6J1K5D1 (peptidyl-tRNA hydrolase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=3 SV=1)

HSP 1 Score: 448 bits (1153), Expect = 2.76e-158
Identity = 223/227 (98.24%), Postives = 225/227 (99.12%), Query Frame = 0


Query: 47  MTSSFTASMSTSSPLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDA 106
           MTSS TASMSTSSPLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEM+DA
Sbjct: 1   MTSSITASMSTSSPLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMLDA 60

Query: 107 VAEVEGISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLV 166
           VAEVEGISMSS SFKALIGKGFIGDVP+MLAKPQTFMNASGESVGAIVSYYKIPLKQVLV
Sbjct: 61  VAEVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNASGESVGAIVSYYKIPLKQVLV 120

Query: 167 IFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVM 226
           IFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVM
Sbjct: 121 IFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVM 180

Query: 227 RPFNKQEREELDFTMQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 273
           RPFNKQEREELDFTMQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG
Sbjct: 181 RPFNKQEREELDFTMQVGVDAIRILLLEGFNKSATFVNTNKPLEQIG 227

[Close]

BLAST of Cp4.1LG09g11480 vs. TAIR 10
Match: AT1G18440.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.1e-96
Identity = 183/280 (65.36%), Postives = 215/280 (76.79%), Query Frame = 0


Query: 8   SSVAALRFP--ICLHCFRTPRTPIS--FPYLSLNPGSLHI---------FASMTSSFTAS 67
           + +A L FP   C   FR+P T +S   P   L  G   +          +S + SF+++
Sbjct: 8   AKIANLSFPSNCCSLFFRSPATFLSPALPCRKLTKGIRGLEGLMSQCLSSSSQSLSFSSN 67

Query: 68  MSTSSPLAPDQLAVEEKKPAPPP--KPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEG 127
             +S P +    A+   KP  PP   PWLIVGLGNPGKKY+ TRHNVGFEMVDA+A+ EG
Sbjct: 68  SFSSQPESELLQALPSSKPKSPPLQLPWLIVGLGNPGKKYQGTRHNVGFEMVDALADAEG 127

Query: 128 ISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLD 187
           ISM++V+FKAL GKG IG++PIMLAKPQTFMN SGESVG IVS+YKIPLKQVLV++DDLD
Sbjct: 128 ISMNTVNFKALFGKGVIGNIPIMLAKPQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLD 187

Query: 188 LPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQ 247
           LPF KLR+LPKGGHGGHNGMRSIID LKGSRDFPRLRIGIGRPPGKMD ANFV+R FN+Q
Sbjct: 188 LPFGKLRLLPKGGHGGHNGMRSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQ 247

Query: 248 EREELDFTMQVGVDAIRILLLEGFNKSATFVNTNKPLEQI 273
           E+EELD T Q G++AIRILLLEGFNKSATFVNT K +EQ+
Sbjct: 248 EQEELDHTFQTGLEAIRILLLEGFNKSATFVNTRKSMEQL 287

[Close]

BLAST of Cp4.1LG09g11480 vs. TAIR 10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 232.3 bits (591), Expect = 4.8e-61
Identity = 99/195 (50.77%), Postives = 146/195 (74.87%), Query Frame = 0


Query: 78  KPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLA 137
           +PWL +GLGNPG KY+ TRHN+GFEM+D  AE  GI M+ V+FKA++G+GF+ D+P++LA
Sbjct: 17  RPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVILA 76

Query: 138 KPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIID 197
           KPQT+MN SGES G + +YYK+PL +VLV+ DD+ LP   LR+  KGGHG HNG++S+++
Sbjct: 77  KPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPCGVLRLQEKGGHGCHNGLKSVMN 136

Query: 198 HLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFN 257
           H +G+R+F RLRIGIG+PPG+MD   F+++ F+   RE +D  +  GVDA++++L + F 
Sbjct: 137 HFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARERMDKALAEGVDALKLVLAKDFG 196

Query: 258 KSATFVNTNKPLEQI 273
           +S    N  +  + +
Sbjct: 197 ESWRLFNVEQKYKHL 211

[Close]

BLAST of Cp4.1LG09g11480 vs. TAIR 10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-59
Identity = 100/186 (53.76%), Postives = 143/186 (76.88%), Query Frame = 0


Query: 79  PWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLAK 138
           PWLIVGLGNPG KY  TRHNVGFEM+D +A  EG+ M+++  KALIG G I DVPI+LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 139 PQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDH 198
           PQT+MN SGESVG++ S+Y++PL+ +L+I+D++ LP   LR+ PKGG G HNG++S++ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 199 LKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFNK 258
           L G R+FPRL IGIG+PPG MD   F+++ F+  E+++++  ++ G +A++ L+L GFN+
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 259 SATFVN 265
             +  N
Sbjct: 225 GISRFN 230

[Close]

BLAST of Cp4.1LG09g11480 vs. TAIR 10
Match: AT5G16140.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-59
Identity = 100/186 (53.76%), Postives = 143/186 (76.88%), Query Frame = 0


Query: 79  PWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEGISMSSVSFKALIGKGFIGDVPIMLAK 138
           PWLIVGLGNPG KY  TRHNVGFEM+D +A  EG+ M+++  KALIG G I DVPI+LAK
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAK 104

Query: 139 PQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNGMRSIIDH 198
           PQT+MN SGESVG++ S+Y++PL+ +L+I+D++ LP   LR+ PKGG G HNG++S++ H
Sbjct: 105 PQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGVKSVMGH 164

Query: 199 LKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQEREELDFTMQVGVDAIRILLLEGFNK 258
           L G R+FPRL IGIG+PPG MD   F+++ F+  E+++++  ++ G +A++ L+L GFN+
Sbjct: 165 LDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLVLNGFNQ 224

Query: 259 SATFVN 265
             +  N
Sbjct: 225 GISRFN 230

[Close]

BLAST of Cp4.1LG09g11480 vs. TAIR 10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.2e-56
Identity = 101/212 (47.64%), Postives = 148/212 (69.81%), Query Frame = 0


Query: 53  ASMSTSSPLAPDQLAVEEKKPAPPPKPWLIVGLGNPGKKYERTRHNVGFEMVDAVAEVEG 112
           +S+  S P++ ++L   E        PWLIVGLGNPG KY  TRHN+GFEM+D +A    
Sbjct: 35  SSLRASLPVSDNKLLKFEY------TPWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATD 94

Query: 113 ISMSSVSFKALIGKGFIGDVPIMLAKPQTFMNASGESVGAIVSYYKIPLKQVLVIFDDLD 172
           ISM+++  KAL+G G +G+VPI+L KPQ +MN SGESVG + +YY+IPL+ +L+I+DD+ 
Sbjct: 95  ISMNTIQSKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMG 154

Query: 173 LPFAKLRMLPKGGHGGHNGMRSIIDHLKGSRDFPRLRIGIGRPPGKMDAANFVMRPFNKQ 232
           L    LR+ PKGGH  HNG++++ +HL G R +PRL IGIG PPG MD   F+++ F+  
Sbjct: 155 LSNGVLRLQPKGGHSQHNGLKNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPL 214

Query: 233 EREELDFTMQVGVDAIRILLLEGFNKSATFVN 265
           ER+++D  ++ GV+ ++ L+ EGF+ S +  N
Sbjct: 215 ERKQMDEGLEQGVEGVKTLVEEGFSDSISRFN 240

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g11480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GW64	2.9e-95	65.36	Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g18...	[more]
Q5N9Q7	3.8e-87	75.90	Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6NLS8	6.7e-60	50.77	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19...	[more]
Q9M5P4	1.3e-58	48.21	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea m...	[more]
Q9LF14	1.7e-58	53.76	Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Ara...	[more]

BLAST of Cp4.1LG09g11480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023541955.1	8.34e-196	100.00	peptidyl-tRNA hydrolase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022994507.1	3.22e-184	94.87	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima]	[more]
XP_022954858.1	1.31e-183	94.51	peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
KAG6572934.1	2.64e-183	94.14	Peptidyl-tRNA hydrolase, chloroplastic, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_038893864.1	1.29e-168	88.32	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Benincasa hispida] >XP_038893...	[more]

BLAST of Cp4.1LG09g11480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JW15	1.56e-184	94.87	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1GS97	6.34e-184	94.51	peptidyl-tRNA hydrolase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=...	[more]
A0A1S3C5R3	1.22e-163	85.71	peptidyl-tRNA hydrolase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103496915 P...	[more]
A0A0A0LVQ7	8.20e-162	84.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042230 PE=3 SV=1	[more]
A0A6J1K5D1	2.76e-158	98.24	peptidyl-tRNA hydrolase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of Cp4.1LG09g11480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18440.1	2.1e-96	65.36	Peptidyl-tRNA hydrolase family protein	[more]
AT5G19830.1	4.8e-61	50.77	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.1	1.2e-59	53.76	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.2	1.2e-59	53.76	Peptidyl-tRNA hydrolase family protein	[more]
AT5G38290.2	1.2e-56	47.64	Peptidyl-tRNA hydrolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036416	Peptidyl-tRNA hydrolase superfamily	GENE3D	3.40.50.1470		coord: 67..266 e-value: 4.9E-74 score: 250.0
IPR036416	Peptidyl-tRNA hydrolase superfamily	SUPERFAMILY	53178	Peptidyl-tRNA hydrolase-like	coord: 79..264
IPR001328	Peptidyl-tRNA hydrolase	TIGRFAM	TIGR00447	TIGR00447	coord: 81..260 e-value: 4.0E-56 score: 187.7
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 81..259 e-value: 4.3E-62 score: 209.2
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 52..268
IPR001328	Peptidyl-tRNA hydrolase	HAMAP	MF_00083	Pept_tRNA_hydro_bact	coord: 79..257 score: 33.786148
None	No IPR available	PANTHER	PTHR17224:SF5	PEPTIDYL-TRNA HYDROLASE CHLOROPLASTIC	coord: 52..268
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01196	PEPT_TRNA_HYDROL_2	coord: 185..195
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01195	PEPT_TRNA_HYDROL_1	coord: 92..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g11480.1	Cp4.1LG09g11480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity