Cp4.1LG09g09140 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g09140
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAminomethyltransferase
LocationCp4.1LG09: 8286403 .. 8289190 (+)
RNA-Seq ExpressionCp4.1LG09g09140
SyntenyCp4.1LG09g09140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCCTAAACCCTAATTAATGCACAATATTGTTTCAATACCAATGGAAATTATACTAAACACAACCAAGTTAAGATCTTGATCTTCATTTCCATTTCCTAGATCTTAAGCGATCGCCCACCGAACTTGGGTGAGGCATTCTGATACCCTGTACGGAGCTTCATGGGAACGATCCTAGACTTTCAACTGATCTCCGTCGGTCAGCCATAGGATCCCACCACTTTGTCATAAGCGGTGGAGATTCTCCGTTGATGGATTTGGTTTTACATTTTGGGTATAAACACGATCGAAGCAACGGATGAATGGGCTGGGAGTTTAAAGAGATTGGGGTATGTGTGAGACTGTGCCACCACCATCCATTTTCTTTTGATTATCATTCTTTTGTTCTATATATAAAGCTTCAAGCCTTTTTCTCCTCCTGATAGCTCAACACTGATCCACAAATGCTCTTGTAGCCTCTCAGATTTGTTTGATTGAAGAACTAAATTTGAAAATAACCCATTTCTGTAAGTGATTCCCTCTTGATTTCATGTCTTTTTATCTGCTTACATTGCGTTTTCCCATCTGTGTTCTTATCTTTTCTCGTAACTTCTCTGTTCTTCTTGGGATTGTTCGATTGGTTTTGTGAGTTTTTGGGATTGTTTGATGGGATCTTGGAAATCGATGTTGAATCGCATTCATAAATGTGCTTGATGAAATTTGGTGTTCATGAAAGGGAATTTGGAATTCTGTCTGTAATCTTGTCTGGTTGGATTGTTCTTTAGATGGTCAGTTTATAATTGTTCCAAACGGTGTTTCAAAATTTAATGCGCTTGATGTCTTGCATCTGCAATGTGCCATATGGGTTCTTCATGCTATCTGCTTAAGATCTACGACTTATGCTAAATAGTTTTGTTCATCTGGAGGAATTTGCATATCATTAGCAGCTTGAAAATCGAACTGTTCTTGTTCTTAATTGTGTATGATCCATGACGGCCTTTTTGTTTAAGGGCAGCTCGCCTTTTTTAATTTTAGGAAGTGAGATAGCATTGGTTCTTTCGATAAATCGATGCATGAGCTGAGGCTCCCATGTCAGACCCTTCGACTAAATAGTTTTGTTAATCTAGAGGAACTTGCAAATCATTAGCAGCTTGTAAATCGAACTGTTCTTGTTCTTAATTCGTGTATGATCCATAACGATGATATATCTGAGTTTTGTAGTGTTCATACTGATTTTGCTTCTACTTTTAGGCTTTTAGTTATATCTTCATTAAGGGTTCATGCTGTGGTTTCATTTATAGACTAGCAAGATGAGGGGAGGTCTGTGGCAACTGGGGCAATCGATAACCCGCCGTCTTGCTCAGGCTGATAAGAAATCCGTGGGGCGTCGATTCTTTGCTGCAGAGTCTGAGCTAAAAAAAACTGCTCTATTTGATTTCCATGTTGCTCATGGTGGAAAGATGGTTCCATTTGCTGGGTGGAGTATGCCTATTCAGTACAAGGACTCGATTATGGATTCGACTGTGAACTGCAGGCAAAATGGTGGACTGTTTGATGTTTCTCATATGTGTGGACTGAGCCTCAAGGGGAAGGACTCTATCCCTTTCCTTGAAACACTTGTTATTGCTGATGTTGCTGGGCTTGCTCCTGGAACTGGAACACTTACTGTCTTTACCAATGAAAAGGGTGGAGCTATCGACGATTCGGTGATCACGAAGGTGACCGATGACCACTTATACTTGGTTGTGAATGCGGGGTGCAGAGACAAGGATTTAGCTCACATCGAGGAGCATATGAAGGCCTTCAAGGCGAAAGGTGGAGACGTCTCGTGGCATATTCATGATGAGAGATCTCTTCTAGCACTCCAGGTTTATATTAAATTGTTGAACTCATCGAACCATTCGGTTCAGGTCGATTGCTTTGTTCTGTTCTTTACATGTTTGCTTGATTTGATTGTATACCTAGGGCCCTCTTGCTGCACCAGTTCTACAACATTTGACGAAAGACGATCTGAGCAAGCTTTACTTCGGCGAGTTTCGCATATTGGATATCAATGGAGCTCATTGCTTCCTCACCAGGACAGGGTATGCTTCCTTTCCACTCTGAACGAGCCATGTCGATTTATCATAACATCATTTATCAACTGTTCTTGAGAAAACTGGTTGAAGTCTATTTATGAATCGTTTTGCTTCAAGGCATTCCGACTTTGCGAGTGGTATCGAAAATGGAACACCTTACACTTCTTATACTCCTGAAATACTCAGGTACACAGGTGAAGATGGGTTTGAGATTTCAGTGCCATCAGAGCACGCACTGGATCTTGCCAAAGCAATCCTGGAGAAATCAGAAGGGAAGGTGAGATTGACAGGACTAGGTGCACGAGACAGTCTTCGACTCGAAGCAGGCCTGTGTTTATATGGCAATGACATGGAACAACACACAACGCCAGTCGAGGCGGGGCTAACATGGGCTATAGGAAAAAGAAGGAGAGCAGAAGGCGGTTTTCTCGGTGCCGAGGTGATTCTGAAACAGCTCGAAGAGGGTCCAGCAGTAAGGCGTGTCGGTTTCGTCTCGTCGGGACCTCCTGCAAGAAGCCACAGTGAGATTCAGAATGAGGAAGGGAAAAACATTGGGGAAATCACGAGTGGAGGATTTAGTCCATGCCTGAAGAAGAACATTGCTATGGGTTATGTGAAATCTGGATCACACAAGGGTGGCACCAAAGTGAAGATTGTTATCAGAGGAAAGACCTACGATGGTGCTGTCACTAAAATGCCATTTGTTCCAACAAAATACTACAAGCCGACATAA

mRNA sequence

ACCCTAAACCCTAATTAATGCACAATATTGTTTCAATACCAATGGAAATTATACTAAACACAACCAAGTTAAGATCTTGATCTTCATTTCCATTTCCTAGATCTTAAGCGATCGCCCACCGAACTTGGGTGAGGCATTCTGATACCCTGTACGGAGCTTCATGGGAACGATCCTAGACTTTCAACTGATCTCCGTCGGTCAGCCATAGGATCCCACCACTTTGTCATAAGCGGTGGAGATTCTCCGTTGATGGATTTGGTTTTACATTTTGGGTATAAACACGATCGAAGCAACGGATGAATGGGCTGGGAGTTTAAAGAGATTGGGCTCAACACTGATCCACAAATGCTCTTGTAGCCTCTCAGATTTGTTTGATTGAAGAACTAAATTTGAAAATAACCCATTTCTACTAGCAAGATGAGGGGAGGTCTGTGGCAACTGGGGCAATCGATAACCCGCCGTCTTGCTCAGGCTGATAAGAAATCCGTGGGGCGTCGATTCTTTGCTGCAGAGTCTGAGCTAAAAAAAACTGCTCTATTTGATTTCCATGTTGCTCATGGTGGAAAGATGGTTCCATTTGCTGGGTGGAGTATGCCTATTCAGTACAAGGACTCGATTATGGATTCGACTGTGAACTGCAGGCAAAATGGTGGACTGTTTGATGTTTCTCATATGTGTGGACTGAGCCTCAAGGGGAAGGACTCTATCCCTTTCCTTGAAACACTTGTTATTGCTGATGTTGCTGGGCTTGCTCCTGGAACTGGAACACTTACTGTCTTTACCAATGAAAAGGGTGGAGCTATCGACGATTCGGTGATCACGAAGGTGACCGATGACCACTTATACTTGGTTGTGAATGCGGGGTGCAGAGACAAGGATTTAGCTCACATCGAGGAGCATATGAAGGCCTTCAAGGCGAAAGGTGGAGACGTCTCGTGGCATATTCATGATGAGAGATCTCTTCTAGCACTCCAGGGCCCTCTTGCTGCACCAGTTCTACAACATTTGACGAAAGACGATCTGAGCAAGCTTTACTTCGGCGAGTTTCGCATATTGGATATCAATGGAGCTCATTGCTTCCTCACCAGGACAGGGCATTCCGACTTTGCGAGTGGTATCGAAAATGGAACACCTTACACTTCTTATACTCCTGAAATACTCAGGTACACAGGTGAAGATGGGTTTGAGATTTCAGTGCCATCAGAGCACGCACTGGATCTTGCCAAAGCAATCCTGGAGAAATCAGAAGGGAAGGTGAGATTGACAGGACTAGGTGCACGAGACAGTCTTCGACTCGAAGCAGGCCTGTGTTTATATGGCAATGACATGGAACAACACACAACGCCAGTCGAGGCGGGGCTAACATGGGCTATAGGAAAAAGAAGGAGAGCAGAAGGCGGTTTTCTCGGTGCCGAGGTGATTCTGAAACAGCTCGAAGAGGGTCCAGCAGTAAGGCGTGTCGGTTTCGTCTCGTCGGGACCTCCTGCAAGAAGCCACAGTGAGATTCAGAATGAGGAAGGGAAAAACATTGGGGAAATCACGAGTGGAGGATTTAGTCCATGCCTGAAGAAGAACATTGCTATGGGTTATGTGAAATCTGGATCACACAAGGGTGGCACCAAAGTGAAGATTGTTATCAGAGGAAAGACCTACGATGGTGCTGTCACTAAAATGCCATTTGTTCCAACAAAATACTACAAGCCGACATAA

Coding sequence (CDS)

ATGAGGGGAGGTCTGTGGCAACTGGGGCAATCGATAACCCGCCGTCTTGCTCAGGCTGATAAGAAATCCGTGGGGCGTCGATTCTTTGCTGCAGAGTCTGAGCTAAAAAAAACTGCTCTATTTGATTTCCATGTTGCTCATGGTGGAAAGATGGTTCCATTTGCTGGGTGGAGTATGCCTATTCAGTACAAGGACTCGATTATGGATTCGACTGTGAACTGCAGGCAAAATGGTGGACTGTTTGATGTTTCTCATATGTGTGGACTGAGCCTCAAGGGGAAGGACTCTATCCCTTTCCTTGAAACACTTGTTATTGCTGATGTTGCTGGGCTTGCTCCTGGAACTGGAACACTTACTGTCTTTACCAATGAAAAGGGTGGAGCTATCGACGATTCGGTGATCACGAAGGTGACCGATGACCACTTATACTTGGTTGTGAATGCGGGGTGCAGAGACAAGGATTTAGCTCACATCGAGGAGCATATGAAGGCCTTCAAGGCGAAAGGTGGAGACGTCTCGTGGCATATTCATGATGAGAGATCTCTTCTAGCACTCCAGGGCCCTCTTGCTGCACCAGTTCTACAACATTTGACGAAAGACGATCTGAGCAAGCTTTACTTCGGCGAGTTTCGCATATTGGATATCAATGGAGCTCATTGCTTCCTCACCAGGACAGGGCATTCCGACTTTGCGAGTGGTATCGAAAATGGAACACCTTACACTTCTTATACTCCTGAAATACTCAGGTACACAGGTGAAGATGGGTTTGAGATTTCAGTGCCATCAGAGCACGCACTGGATCTTGCCAAAGCAATCCTGGAGAAATCAGAAGGGAAGGTGAGATTGACAGGACTAGGTGCACGAGACAGTCTTCGACTCGAAGCAGGCCTGTGTTTATATGGCAATGACATGGAACAACACACAACGCCAGTCGAGGCGGGGCTAACATGGGCTATAGGAAAAAGAAGGAGAGCAGAAGGCGGTTTTCTCGGTGCCGAGGTGATTCTGAAACAGCTCGAAGAGGGTCCAGCAGTAAGGCGTGTCGGTTTCGTCTCGTCGGGACCTCCTGCAAGAAGCCACAGTGAGATTCAGAATGAGGAAGGGAAAAACATTGGGGAAATCACGAGTGGAGGATTTAGTCCATGCCTGAAGAAGAACATTGCTATGGGTTATGTGAAATCTGGATCACACAAGGGTGGCACCAAAGTGAAGATTGTTATCAGAGGAAAGACCTACGATGGTGCTGTCACTAAAATGCCATTTGTTCCAACAAAATACTACAAGCCGACATAA

Protein sequence

MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMPIQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTVFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDERSLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPYTSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLYGNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSHSEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMPFVPTKYYKPT
Homology
BLAST of Cp4.1LG09g09140 vs. ExPASy Swiss-Prot
Match: P54260 (Aminomethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 GN=GDCST PE=2 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 5.7e-215
Identity = 363/429 (84.62%), Postives = 392/429 (91.38%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKK++GRR FA++++LKKT L+DFHV +GGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKTIGRRCFASDADLKKTVLYDFHVVNGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCR+NG LFDVSHMCGLSLKGKD+IPFLE LVIADVAGLAPGTG+LTV
Sbjct: 61  IQYKDSIMDSTVNCRENGSLFDVSHMCGLSLKGKDTIPFLEKLVIADVAGLAPGTGSLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSV+TKVT+DH+YLVVNAGCRDKDLAHIEEHMK+FK+KGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVVTKVTNDHIYLVVNAGCRDKDLAHIEEHMKSFKSKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQ+LTKDDLSK+YFGEFR+LDINGA CFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQYLTKDDLSKMYFGEFRVLDINGAPCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSE+ALDLAKA+LEKSEGK+RLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSENALDLAKALLEKSEGKIRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQ+EEGP +RRVGF SSGPP RSH
Sbjct: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQIEEGPKIRRVGFFSSGPPPRSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQ+  G+NIGEITSGGFSPCLKKNIAMGYVK+G+HK GT VKIVIRGK+YDG VTKMP
Sbjct: 361 SEIQDSNGQNIGEITSGGFSPCLKKNIAMGYVKTGNHKAGTNVKIVIRGKSYDGVVTKMP 406

Query: 421 FVPTKYYKP 430
           FVPTKYYKP
Sbjct: 421 FVPTKYYKP 406

BLAST of Cp4.1LG09g09140 vs. ExPASy Swiss-Prot
Match: O49849 (Aminomethyltransferase, mitochondrial OS=Flaveria anomala OX=35877 GN=GDCST PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 4.3e-210
Identity = 354/429 (82.52%), Postives = 387/429 (90.21%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRL Q+DKK++ RR +A+E++LKKT L+DFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLGQSDKKTIVRRCYASEADLKKTVLYDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDST+NCR+NG LFDVSHMCGLSLKGKD + FLE LV+ADVAGLAPGTG+LTV
Sbjct: 61  IQYKDSIMDSTINCRENGSLFDVSHMCGLSLKGKDCVAFLEKLVVADVAGLAPGTGSLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDH+YLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHI+DER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIYDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLA   LQHLTK+DLSK+YFG+FRI+DING+ CFLTRTG              
Sbjct: 181 SLLALQGPLAGSTLQHLTKEDLSKMYFGDFRIIDINGSKCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSE+A+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSENAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQH TPVEAGLTWAIGKRRRAEGGFLGA+VILKQ+ +GPA+RRVG  S+GPPARSH
Sbjct: 301 GNDMEQHITPVEAGLTWAIGKRRRAEGGFLGADVILKQIADGPAIRRVGLFSTGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNE+G+NIGE+TSGGFSPCLKKNI MGYVKSG HK GTK+KIVIRGKTY+G+VTKMP
Sbjct: 361 SEIQNEKGENIGEVTSGGFSPCLKKNIGMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMP 406

Query: 421 FVPTKYYKP 430
           FVPTKYYKP
Sbjct: 421 FVPTKYYKP 406

BLAST of Cp4.1LG09g09140 vs. ExPASy Swiss-Prot
Match: O65396 (Aminomethyltransferase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GDCST PE=2 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 7.3e-210
Identity = 357/431 (82.83%), Postives = 388/431 (90.02%), Query Frame = 0

Query: 1   MRGG-LWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSM 60
           MRGG LWQLGQSITRRLAQ+DKK V RR+FA+E++LKKTAL+DFHVAHGGKMVPFAGWSM
Sbjct: 1   MRGGSLWQLGQSITRRLAQSDKKVVSRRYFASEADLKKTALYDFHVAHGGKMVPFAGWSM 60

Query: 61  PIQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLT 120
           PIQYKDSIMDSTVNCR+NG LFDV+HMCGLSLKGKD +PFLETLV+ADVAGLAPGTG+LT
Sbjct: 61  PIQYKDSIMDSTVNCRENGSLFDVAHMCGLSLKGKDCVPFLETLVVADVAGLAPGTGSLT 120

Query: 121 VFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDE 180
           VFTNEKGGAIDDSVITKVTD+H+YLVVNAGCRDKDLAHIEEHMKAFK+KGGDVSWHIHDE
Sbjct: 121 VFTNEKGGAIDDSVITKVTDEHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDE 180

Query: 181 RSLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTP 240
           RSLLALQGPLAAPVLQHLTK+DLSKLYFG F+ILDING+ CFLTRTG             
Sbjct: 181 RSLLALQGPLAAPVLQHLTKEDLSKLYFGNFQILDINGSTCFLTRTG------------- 240

Query: 241 YTSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300
                     YTGEDGFEISVP EHA+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL
Sbjct: 241 ----------YTGEDGFEISVPDEHAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300

Query: 301 YGNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARS 360
           YGNDMEQH +PVEAGLTWAIGKRRRAEGGFLGA+VIL+QL++GP +RRVGF SSGPPARS
Sbjct: 301 YGNDMEQHISPVEAGLTWAIGKRRRAEGGFLGADVILQQLKDGPTIRRVGFFSSGPPARS 360

Query: 361 HSEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKM 420
           HSE+ +E G  IGEITSGGFSP LKKNIAMGYVKSG HK GTKVKI++RGK Y+G++TKM
Sbjct: 361 HSEVHDESGNKIGEITSGGFSPNLKKNIAMGYVKSGQHKTGTKVKILVRGKPYEGSITKM 408

Query: 421 PFVPTKYYKPT 431
           PFV TKYYKPT
Sbjct: 421 PFVATKYYKPT 408

BLAST of Cp4.1LG09g09140 vs. ExPASy Swiss-Prot
Match: P49364 (Aminomethyltransferase, mitochondrial OS=Pisum sativum OX=3888 GN=GDCST PE=1 SV=2)

HSP 1 Score: 731.1 bits (1886), Expect = 7.3e-210
Identity = 359/431 (83.29%), Postives = 383/431 (88.86%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQ-ADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSM 60
           MRGGLWQLGQSITRRLA   DKK+V RR FA ESELKKT L+DFHVAHGGKMVPFAGWSM
Sbjct: 1   MRGGLWQLGQSITRRLANGGDKKAVARRCFATESELKKTVLYDFHVAHGGKMVPFAGWSM 60

Query: 61  PIQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLT 120
           PIQYKDSIMDST+NCRQNG LFDVSHMCGLSLKGKD + FLE LVIADVA LA GTGTLT
Sbjct: 61  PIQYKDSIMDSTLNCRQNGSLFDVSHMCGLSLKGKDVVSFLEKLVIADVAALAHGTGTLT 120

Query: 121 VFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDE 180
           VFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDE
Sbjct: 121 VFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDE 180

Query: 181 RSLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTP 240
           RSLLALQGPLAAPVLQHLTK+DLSKLYFGEFR+LDING+ CFLTRTG             
Sbjct: 181 RSLLALQGPLAAPVLQHLTKEDLSKLYFGEFRVLDINGSQCFLTRTG------------- 240

Query: 241 YTSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300
                     YTGEDGFEISVPSEH ++LAKA+LEKSEGK+RLTGLGARDSLRLEAGLCL
Sbjct: 241 ----------YTGEDGFEISVPSEHGVELAKALLEKSEGKIRLTGLGARDSLRLEAGLCL 300

Query: 301 YGNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARS 360
           YGND+EQH TP+EAGLTWAIGKRRRAEGGFLGA+VILKQL +GP++RRVGF+SSGPP RS
Sbjct: 301 YGNDLEQHITPIEAGLTWAIGKRRRAEGGFLGADVILKQLADGPSIRRVGFISSGPPPRS 360

Query: 361 HSEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKM 420
           HSEIQ+E G NIGE+TSGGFSPCLKKNIA+GYVKSG HK GTKVKI+IRGK  +G VTKM
Sbjct: 361 HSEIQDEGGNNIGEVTSGGFSPCLKKNIAIGYVKSGLHKAGTKVKIIIRGKQNEGVVTKM 408

Query: 421 PFVPTKYYKPT 431
           PFVPTKYYKP+
Sbjct: 421 PFVPTKYYKPS 408

BLAST of Cp4.1LG09g09140 vs. ExPASy Swiss-Prot
Match: P49363 (Aminomethyltransferase, mitochondrial OS=Flaveria pringlei OX=4226 GN=GDCST PE=2 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 1.6e-209
Identity = 352/429 (82.05%), Postives = 387/429 (90.21%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRL Q+DKK++ RR +A+E++LKKT L+DFHVA+GGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLGQSDKKTIARRCYASEADLKKTVLYDFHVANGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIM+ST+NCR+NG LFDVSHMCGLSLKGKD +PFLE LV+ADVAGL PGTG+LTV
Sbjct: 61  IQYKDSIMESTINCRENGSLFDVSHMCGLSLKGKDCVPFLEKLVVADVAGLRPGTGSLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDH+YLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHI+DER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIYDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLA   LQHLTK+DLSK+YFG+FRI+DING+ CFLTRTG              
Sbjct: 181 SLLALQGPLAGSTLQHLTKEDLSKMYFGDFRIIDINGSKCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSE+A+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSENAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQH TPVEAGLTWAIGKRRRAEGGFLGA+VILKQ+ +GPA+RRVG  S+GPPARSH
Sbjct: 301 GNDMEQHITPVEAGLTWAIGKRRRAEGGFLGADVILKQIADGPAIRRVGLFSTGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNE+G+NIGE+TSGGFSPCLKKNI MGYVKSG HK GTK+KIVIRGKTY+G+VTKMP
Sbjct: 361 SEIQNEKGENIGEVTSGGFSPCLKKNIGMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMP 406

Query: 421 FVPTKYYKP 430
           FVPTKYYKP
Sbjct: 421 FVPTKYYKP 406

BLAST of Cp4.1LG09g09140 vs. NCBI nr
Match: XP_023542681.1 (aminomethyltransferase, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023542682.1 aminomethyltransferase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 810 bits (2092), Expect = 1.93e-295
Identity = 407/430 (94.65%), Postives = 407/430 (94.65%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH
Sbjct: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP
Sbjct: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. NCBI nr
Match: XP_022954805.1 (aminomethyltransferase, mitochondrial [Cucurbita moschata] >XP_022954806.1 aminomethyltransferase, mitochondrial [Cucurbita moschata] >XP_022954807.1 aminomethyltransferase, mitochondrial [Cucurbita moschata] >XP_022954808.1 aminomethyltransferase, mitochondrial [Cucurbita moschata] >KAG6573179.1 Aminomethyltransferase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808 bits (2086), Expect = 1.59e-294
Identity = 406/430 (94.42%), Postives = 406/430 (94.42%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH
Sbjct: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHK GTKVKIVIRGKTYDGAVTKMP
Sbjct: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKAGTKVKIVIRGKTYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. NCBI nr
Match: KAG7012358.1 (Aminomethyltransferase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 806 bits (2083), Expect = 4.55e-294
Identity = 405/430 (94.19%), Postives = 406/430 (94.42%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRL+QADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLSQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH
Sbjct: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHK GTKVKIVIRGKTYDGAVTKMP
Sbjct: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKAGTKVKIVIRGKTYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. NCBI nr
Match: XP_022994194.1 (aminomethyltransferase, mitochondrial [Cucurbita maxima] >XP_022994195.1 aminomethyltransferase, mitochondrial [Cucurbita maxima] >XP_022994196.1 aminomethyltransferase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 804 bits (2076), Expect = 5.31e-293
Identity = 405/430 (94.19%), Postives = 405/430 (94.19%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH
Sbjct: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGEITSGGFSP LKKNIAMGYVKSGSHK GTKVKIVIRGKTYDGAVTKMP
Sbjct: 361 SEIQNEEGKNIGEITSGGFSPSLKKNIAMGYVKSGSHKAGTKVKIVIRGKTYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. NCBI nr
Match: XP_038894763.1 (aminomethyltransferase, mitochondrial [Benincasa hispida] >XP_038894765.1 aminomethyltransferase, mitochondrial [Benincasa hispida])

HSP 1 Score: 787 bits (2033), Expect = 1.90e-286
Identity = 390/430 (90.70%), Postives = 399/430 (92.79%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKK+VGRRFF+ ESELKKT L+DFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKAVGRRFFSTESELKKTVLYDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIM+STVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLV+ADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMESTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVVADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDH+YLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHIYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHALDLAKAILEKSE KVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHALDLAKAILEKSEAKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQH TPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPA+RRVGF SSGPPARSH
Sbjct: 301 GNDMEQHITPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAIRRVGFFSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGE+TSGGFSPCLKKNIAMGYVKSGSHK GTKVKI+IRGK YDG VTKMP
Sbjct: 361 SEIQNEEGKNIGEVTSGGFSPCLKKNIAMGYVKSGSHKAGTKVKIIIRGKAYDGVVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. ExPASy TrEMBL
Match: A0A6J1GS47 (Aminomethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456958 PE=3 SV=1)

HSP 1 Score: 808 bits (2086), Expect = 7.68e-295
Identity = 406/430 (94.42%), Postives = 406/430 (94.42%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH
Sbjct: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHK GTKVKIVIRGKTYDGAVTKMP
Sbjct: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKAGTKVKIVIRGKTYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. ExPASy TrEMBL
Match: A0A6J1K0K4 (Aminomethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111490006 PE=3 SV=1)

HSP 1 Score: 804 bits (2076), Expect = 2.57e-293
Identity = 405/430 (94.19%), Postives = 405/430 (94.19%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH
Sbjct: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGEITSGGFSP LKKNIAMGYVKSGSHK GTKVKIVIRGKTYDGAVTKMP
Sbjct: 361 SEIQNEEGKNIGEITSGGFSPSLKKNIAMGYVKSGSHKAGTKVKIVIRGKTYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. ExPASy TrEMBL
Match: A0A6J1KQ11 (Aminomethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495448 PE=3 SV=1)

HSP 1 Score: 781 bits (2018), Expect = 1.77e-284
Identity = 388/430 (90.23%), Postives = 399/430 (92.79%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQ+DKK+VGRRFF+ ESELKKT L+DFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQSDKKAVGRRFFSGESELKKTVLYDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLE+LVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLESLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAP LQ LTKDDLSKLYFGEFRILDINGAHC+LTRTG              
Sbjct: 181 SLLALQGPLAAPSLQQLTKDDLSKLYFGEFRILDINGAHCYLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHA+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQH TPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPA+RRVGF SSGPPARSH
Sbjct: 301 GNDMEQHITPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAIRRVGFFSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGK+IGE+TSGGFSPCLKKNIAMGYVKSGSHK GTKVKI+IRGK YDGAVTKMP
Sbjct: 361 SEIQNEEGKSIGEVTSGGFSPCLKKNIAMGYVKSGSHKAGTKVKIIIRGKAYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. ExPASy TrEMBL
Match: A0A6J1E8P4 (Aminomethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111431648 PE=3 SV=1)

HSP 1 Score: 781 bits (2018), Expect = 1.77e-284
Identity = 388/430 (90.23%), Postives = 399/430 (92.79%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQ+DKK+VGRRFF+ ESELKKT L+DFHVAHGGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQSDKKAVGRRFFSGESELKKTVLYDFHVAHGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLE+LVIADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLESLVIADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SLLALQGPLAAP LQ LTKDDLSKLYFGEFRILDINGAHC+LTRTG              
Sbjct: 181 SLLALQGPLAAPSLQQLTKDDLSKLYFGEFRILDINGAHCYLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHA+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQH TPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPA+RRVGF SSGPPARSH
Sbjct: 301 GNDMEQHITPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAIRRVGFFSSGPPARSH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGK+IGE+TSGGFSPCLKKNIAMGYVKSGSHK GTKVKI+IRGK YDGAVTKMP
Sbjct: 361 SEIQNEEGKSIGEVTSGGFSPCLKKNIAMGYVKSGSHKAGTKVKIIIRGKAYDGAVTKMP 407

Query: 421 FVPTKYYKPT 430
           FVPTKYYKPT
Sbjct: 421 FVPTKYYKPT 407

BLAST of Cp4.1LG09g09140 vs. ExPASy TrEMBL
Match: A0A6J1CEI1 (Aminomethyltransferase OS=Momordica charantia OX=3673 GN=LOC111010769 PE=3 SV=1)

HSP 1 Score: 777 bits (2006), Expect = 1.20e-282
Identity = 386/429 (89.98%), Postives = 396/429 (92.31%), Query Frame = 0

Query: 1   MRGGLWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSMP 60
           MRGGLWQLGQSITRRLAQADKK+VGRRFF+AESELKKTAL+DFHVA+GGKMVPFAGWSMP
Sbjct: 1   MRGGLWQLGQSITRRLAQADKKAVGRRFFSAESELKKTALYDFHVANGGKMVPFAGWSMP 60

Query: 61  IQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLTV 120
           IQYKDSIMDSTVNCRQNGGLFDV+HMCGLSLKGKD IPFLETLV+ADVAGLAPGTGTLTV
Sbjct: 61  IQYKDSIMDSTVNCRQNGGLFDVAHMCGLSLKGKDCIPFLETLVVADVAGLAPGTGTLTV 120

Query: 121 FTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDER 180
           FTNEKGGAIDDSVITKV DDH+YLVVNAGCRDKDLAHIE HMKAFKAKGGDVSWHIHDER
Sbjct: 121 FTNEKGGAIDDSVITKVKDDHIYLVVNAGCRDKDLAHIEHHMKAFKAKGGDVSWHIHDER 180

Query: 181 SLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTPY 240
           SL+ALQGPLAAPVLQHLTK+DLSKLYFGEFRILDINGAHCFLTRTG              
Sbjct: 181 SLIALQGPLAAPVLQHLTKEDLSKLYFGEFRILDINGAHCFLTRTG-------------- 240

Query: 241 TSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300
                    YTGEDGFEISVPSEHA+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY
Sbjct: 241 ---------YTGEDGFEISVPSEHAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCLY 300

Query: 301 GNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARSH 360
           GNDMEQHTTPVEAGL WAIGKRRRAEGGFLGAEVILKQL EGPAVRRVGF SSGPPAR H
Sbjct: 301 GNDMEQHTTPVEAGLAWAIGKRRRAEGGFLGAEVILKQLAEGPAVRRVGFFSSGPPARGH 360

Query: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKMP 420
           SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHK GTKVKI IRGKTYDG VTKMP
Sbjct: 361 SEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKAGTKVKIAIRGKTYDGVVTKMP 406

Query: 421 FVPTKYYKP 429
           FVPTKYYKP
Sbjct: 421 FVPTKYYKP 406

BLAST of Cp4.1LG09g09140 vs. TAIR 10
Match: AT1G11860.1 (Glycine cleavage T-protein family )

HSP 1 Score: 731.1 bits (1886), Expect = 5.2e-211
Identity = 357/431 (82.83%), Postives = 388/431 (90.02%), Query Frame = 0

Query: 1   MRGG-LWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSM 60
           MRGG LWQLGQSITRRLAQ+DKK V RR+FA+E++LKKTAL+DFHVAHGGKMVPFAGWSM
Sbjct: 1   MRGGSLWQLGQSITRRLAQSDKKVVSRRYFASEADLKKTALYDFHVAHGGKMVPFAGWSM 60

Query: 61  PIQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLT 120
           PIQYKDSIMDSTVNCR+NG LFDV+HMCGLSLKGKD +PFLETLV+ADVAGLAPGTG+LT
Sbjct: 61  PIQYKDSIMDSTVNCRENGSLFDVAHMCGLSLKGKDCVPFLETLVVADVAGLAPGTGSLT 120

Query: 121 VFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDE 180
           VFTNEKGGAIDDSVITKVTD+H+YLVVNAGCRDKDLAHIEEHMKAFK+KGGDVSWHIHDE
Sbjct: 121 VFTNEKGGAIDDSVITKVTDEHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDE 180

Query: 181 RSLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTP 240
           RSLLALQGPLAAPVLQHLTK+DLSKLYFG F+ILDING+ CFLTRTG             
Sbjct: 181 RSLLALQGPLAAPVLQHLTKEDLSKLYFGNFQILDINGSTCFLTRTG------------- 240

Query: 241 YTSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300
                     YTGEDGFEISVP EHA+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL
Sbjct: 241 ----------YTGEDGFEISVPDEHAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300

Query: 301 YGNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARS 360
           YGNDMEQH +PVEAGLTWAIGKRRRAEGGFLGA+VIL+QL++GP +RRVGF SSGPPARS
Sbjct: 301 YGNDMEQHISPVEAGLTWAIGKRRRAEGGFLGADVILQQLKDGPTIRRVGFFSSGPPARS 360

Query: 361 HSEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKM 420
           HSE+ +E G  IGEITSGGFSP LKKNIAMGYVKSG HK GTKVKI++RGK Y+G++TKM
Sbjct: 361 HSEVHDESGNKIGEITSGGFSPNLKKNIAMGYVKSGQHKTGTKVKILVRGKPYEGSITKM 408

Query: 421 PFVPTKYYKPT 431
           PFV TKYYKPT
Sbjct: 421 PFVATKYYKPT 408

BLAST of Cp4.1LG09g09140 vs. TAIR 10
Match: AT1G11860.2 (Glycine cleavage T-protein family )

HSP 1 Score: 731.1 bits (1886), Expect = 5.2e-211
Identity = 357/431 (82.83%), Postives = 388/431 (90.02%), Query Frame = 0

Query: 1   MRGG-LWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSM 60
           MRGG LWQLGQSITRRLAQ+DKK V RR+FA+E++LKKTAL+DFHVAHGGKMVPFAGWSM
Sbjct: 1   MRGGSLWQLGQSITRRLAQSDKKVVSRRYFASEADLKKTALYDFHVAHGGKMVPFAGWSM 60

Query: 61  PIQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLT 120
           PIQYKDSIMDSTVNCR+NG LFDV+HMCGLSLKGKD +PFLETLV+ADVAGLAPGTG+LT
Sbjct: 61  PIQYKDSIMDSTVNCRENGSLFDVAHMCGLSLKGKDCVPFLETLVVADVAGLAPGTGSLT 120

Query: 121 VFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDE 180
           VFTNEKGGAIDDSVITKVTD+H+YLVVNAGCRDKDLAHIEEHMKAFK+KGGDVSWHIHDE
Sbjct: 121 VFTNEKGGAIDDSVITKVTDEHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDE 180

Query: 181 RSLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTP 240
           RSLLALQGPLAAPVLQHLTK+DLSKLYFG F+ILDING+ CFLTRTG             
Sbjct: 181 RSLLALQGPLAAPVLQHLTKEDLSKLYFGNFQILDINGSTCFLTRTG------------- 240

Query: 241 YTSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300
                     YTGEDGFEISVP EHA+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL
Sbjct: 241 ----------YTGEDGFEISVPDEHAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300

Query: 301 YGNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARS 360
           YGNDMEQH +PVEAGLTWAIGKRRRAEGGFLGA+VIL+QL++GP +RRVGF SSGPPARS
Sbjct: 301 YGNDMEQHISPVEAGLTWAIGKRRRAEGGFLGADVILQQLKDGPTIRRVGFFSSGPPARS 360

Query: 361 HSEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKM 420
           HSE+ +E G  IGEITSGGFSP LKKNIAMGYVKSG HK GTKVKI++RGK Y+G++TKM
Sbjct: 361 HSEVHDESGNKIGEITSGGFSPNLKKNIAMGYVKSGQHKTGTKVKILVRGKPYEGSITKM 408

Query: 421 PFVPTKYYKPT 431
           PFV TKYYKPT
Sbjct: 421 PFVATKYYKPT 408

BLAST of Cp4.1LG09g09140 vs. TAIR 10
Match: AT1G11860.3 (Glycine cleavage T-protein family )

HSP 1 Score: 731.1 bits (1886), Expect = 5.2e-211
Identity = 357/431 (82.83%), Postives = 388/431 (90.02%), Query Frame = 0

Query: 1   MRGG-LWQLGQSITRRLAQADKKSVGRRFFAAESELKKTALFDFHVAHGGKMVPFAGWSM 60
           MRGG LWQLGQSITRRLAQ+DKK V RR+FA+E++LKKTAL+DFHVAHGGKMVPFAGWSM
Sbjct: 1   MRGGSLWQLGQSITRRLAQSDKKVVSRRYFASEADLKKTALYDFHVAHGGKMVPFAGWSM 60

Query: 61  PIQYKDSIMDSTVNCRQNGGLFDVSHMCGLSLKGKDSIPFLETLVIADVAGLAPGTGTLT 120
           PIQYKDSIMDSTVNCR+NG LFDV+HMCGLSLKGKD +PFLETLV+ADVAGLAPGTG+LT
Sbjct: 61  PIQYKDSIMDSTVNCRENGSLFDVAHMCGLSLKGKDCVPFLETLVVADVAGLAPGTGSLT 120

Query: 121 VFTNEKGGAIDDSVITKVTDDHLYLVVNAGCRDKDLAHIEEHMKAFKAKGGDVSWHIHDE 180
           VFTNEKGGAIDDSVITKVTD+H+YLVVNAGCRDKDLAHIEEHMKAFK+KGGDVSWHIHDE
Sbjct: 121 VFTNEKGGAIDDSVITKVTDEHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDE 180

Query: 181 RSLLALQGPLAAPVLQHLTKDDLSKLYFGEFRILDINGAHCFLTRTGHSDFASGIENGTP 240
           RSLLALQGPLAAPVLQHLTK+DLSKLYFG F+ILDING+ CFLTRTG             
Sbjct: 181 RSLLALQGPLAAPVLQHLTKEDLSKLYFGNFQILDINGSTCFLTRTG------------- 240

Query: 241 YTSYTPEILRYTGEDGFEISVPSEHALDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300
                     YTGEDGFEISVP EHA+DLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL
Sbjct: 241 ----------YTGEDGFEISVPDEHAVDLAKAILEKSEGKVRLTGLGARDSLRLEAGLCL 300

Query: 301 YGNDMEQHTTPVEAGLTWAIGKRRRAEGGFLGAEVILKQLEEGPAVRRVGFVSSGPPARS 360
           YGNDMEQH +PVEAGLTWAIGKRRRAEGGFLGA+VIL+QL++GP +RRVGF SSGPPARS
Sbjct: 301 YGNDMEQHISPVEAGLTWAIGKRRRAEGGFLGADVILQQLKDGPTIRRVGFFSSGPPARS 360

Query: 361 HSEIQNEEGKNIGEITSGGFSPCLKKNIAMGYVKSGSHKGGTKVKIVIRGKTYDGAVTKM 420
           HSE+ +E G  IGEITSGGFSP LKKNIAMGYVKSG HK GTKVKI++RGK Y+G++TKM
Sbjct: 361 HSEVHDESGNKIGEITSGGFSPNLKKNIAMGYVKSGQHKTGTKVKILVRGKPYEGSITKM 408

Query: 421 PFVPTKYYKPT 431
           PFV TKYYKPT
Sbjct: 421 PFVATKYYKPT 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P542605.7e-21584.62Aminomethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 GN=GDCST PE=2... [more]
O498494.3e-21082.52Aminomethyltransferase, mitochondrial OS=Flaveria anomala OX=35877 GN=GDCST PE=3... [more]
O653967.3e-21082.83Aminomethyltransferase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GDCST P... [more]
P493647.3e-21083.29Aminomethyltransferase, mitochondrial OS=Pisum sativum OX=3888 GN=GDCST PE=1 SV=... [more]
P493631.6e-20982.05Aminomethyltransferase, mitochondrial OS=Flaveria pringlei OX=4226 GN=GDCST PE=2... [more]
Match NameE-valueIdentityDescription
XP_023542681.11.93e-29594.65aminomethyltransferase, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023542682... [more]
XP_022954805.11.59e-29494.42aminomethyltransferase, mitochondrial [Cucurbita moschata] >XP_022954806.1 amino... [more]
KAG7012358.14.55e-29494.19Aminomethyltransferase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022994194.15.31e-29394.19aminomethyltransferase, mitochondrial [Cucurbita maxima] >XP_022994195.1 aminome... [more]
XP_038894763.11.90e-28690.70aminomethyltransferase, mitochondrial [Benincasa hispida] >XP_038894765.1 aminom... [more]
Match NameE-valueIdentityDescription
A0A6J1GS477.68e-29594.42Aminomethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456958 PE=3 SV=1[more]
A0A6J1K0K42.57e-29394.19Aminomethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111490006 PE=3 SV=1[more]
A0A6J1KQ111.77e-28490.23Aminomethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495448 PE=3 SV=1[more]
A0A6J1E8P41.77e-28490.23Aminomethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111431648 PE=3 SV=1[more]
A0A6J1CEI11.20e-28289.98Aminomethyltransferase OS=Momordica charantia OX=3673 GN=LOC111010769 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G11860.15.2e-21182.83Glycine cleavage T-protein family [more]
AT1G11860.25.2e-21182.83Glycine cleavage T-protein family [more]
AT1G11860.35.2e-21182.83Glycine cleavage T-protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027266GTP-binding protein TrmE/Glycine cleavage system T protein, domain 1GENE3D3.30.1360.120Probable tRNA modification gtpase trme; domain 1coord: 36..227
e-value: 1.6E-64
score: 219.5
IPR027266GTP-binding protein TrmE/Glycine cleavage system T protein, domain 1GENE3D3.30.1360.120Probable tRNA modification gtpase trme; domain 1coord: 240..295
e-value: 7.2E-15
score: 57.2
IPR006222Aminomethyltransferase, folate-binding domainPFAMPF01571GCV_Tcoord: 250..318
e-value: 3.5E-25
score: 88.8
coord: 40..228
e-value: 2.6E-56
score: 190.8
IPR013977Glycine cleavage T-protein, C-terminal barrel domainPFAMPF08669GCV_T_Ccoord: 346..422
e-value: 1.9E-17
score: 63.0
NoneNo IPR availableGENE3D4.10.1250.10Aminomethyltransferase fragmentcoord: 296..340
e-value: 2.7E-23
score: 83.3
NoneNo IPR availableGENE3D3.30.70.1400coord: 86..176
e-value: 1.6E-64
score: 219.5
NoneNo IPR availableGENE3D2.40.30.110coord: 344..418
e-value: 5.0E-28
score: 98.3
NoneNo IPR availablePANTHERPTHR43757:SF6AMINOMETHYLTRANSFERASEcoord: 1..229
coord: 249..430
NoneNo IPR availableSUPERFAMILY103025Folate-binding domaincoord: 34..343
IPR028896Aminomethyltransferase-likePIRSFPIRSF006487GCSTcoord: 11..235
e-value: 1.8E-36
score: 123.6
coord: 247..430
e-value: 3.2E-41
score: 139.3
IPR028896Aminomethyltransferase-likePANTHERPTHR43757AMINOMETHYLTRANSFERASEcoord: 1..229
coord: 249..430
IPR029043Glycine cleavage T-protein/YgfZ, C-terminalSUPERFAMILY101790Aminomethyltransferase beta-barrel domaincoord: 335..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g09140.1Cp4.1LG09g09140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019464 glycine decarboxylation via glycine cleavage system
biological_process GO:0032259 methylation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004047 aminomethyltransferase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0008483 transaminase activity