Cp4.1LG09g08540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g08540
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	alpha/beta-Hydrolases superfamily protein
Location	Cp4.1LG09: 7894377 .. 7895375 (+)
RNA-Seq Expression	Cp4.1LG09g08540
Synteny	Cp4.1LG09g08540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCAAACCCATCACCAAAATCCCCAATCCTTTCTGAACTACTGGGGAAACATCTCCGAGGAAGATTATTACGCTTCTCAACGAATCAAATCCTCCAAATCCTTCTACACTTCCCCTCGCGGCCTCAAGCTCTTCACTCGTTCATGGCTTCCTCTGCCTCCCACCCCTCCCCGAGCCCTCATCTTCATGGTCCATGGCTACGGCAACAACATCAGCTGGACCTTCCAAGCCACTGCCATCTTCCTCGCCCAGATGGGTTTCGCCTGCTTCGCTCTCGACCTCGAAGGCCATGGCGGATCCGAAGGTCTCAAAGCCTTCGTCCCTAATGTCGACTCTGTTGTCCACGACTGTCTCTCCTTCTTCCGATTCGTTAAATTGAACCCCCAATTTCAGGGTCTGCCCGCCTTCCTCTTCGGCGAGTCAATGGGCGGTGCGATTTGCTTATTGATTCATTTCGCTGATCCCAAAGGGTTCGAGGGCGCTATTCTCGTCGCACCCATGTGCAAAATCTCTGATAAAGTGAAACCCATTTGGCCGATTCCTCAATTTCTTACGGCGGTTGCGAGGTTTTTGCCGAGTTTGGCGATTGTGCCGACGGCGGATCTTTTGGAGAAGTCGGTGAAGGTGGAGGAGAAGAAGATTGTGGCGGAGATGAATCCGATGAGGTATAGGGGGAAACCCAGATTGGGAACTGTGGTGGAGCTTTTGAGAGTTACAGAGGATTTGAGCGGGAGGCTGGGGGATGTGAAGCTGCCGTTCATCGTCGTCCATGGCGATGCGGATGTTGTTACGGATCCGAATGTGAGCAAATCTCTGTATGAGGAAGCGCAGAGCGAGGACAAGACGATGAAGATTTATGAGGGAATGATGCATTCTATGCTGTTTGGGGAGACGGATGAGAATGTGGAGATTGTTCGAAATGATATTGTTTCTTGGTTGAATGCGAGATGTAGGAGTAGGATAAGTGATGATGATGATGATGATCAGAAGAATTAG

mRNA sequence

ATGTCCCAAACCCATCACCAAAATCCCCAATCCTTTCTGAACTACTGGGGAAACATCTCCGAGGAAGATTATTACGCTTCTCAACGAATCAAATCCTCCAAATCCTTCTACACTTCCCCTCGCGGCCTCAAGCTCTTCACTCGTTCATGGCTTCCTCTGCCTCCCACCCCTCCCCGAGCCCTCATCTTCATGGTCCATGGCTACGGCAACAACATCAGCTGGACCTTCCAAGCCACTGCCATCTTCCTCGCCCAGATGGGTTTCGCCTGCTTCGCTCTCGACCTCGAAGGCCATGGCGGATCCGAAGGTCTCAAAGCCTTCGTCCCTAATGTCGACTCTGTTGTCCACGACTGTCTCTCCTTCTTCCGATTCGTTAAATTGAACCCCCAATTTCAGGGTCTGCCCGCCTTCCTCTTCGGCGAGTCAATGGGCGGTGCGATTTGCTTATTGATTCATTTCGCTGATCCCAAAGGGTTCGAGGGCGCTATTCTCGTCGCACCCATGTGCAAAATCTCTGATAAAGTGAAACCCATTTGGCCGATTCCTCAATTTCTTACGGCGGTTGCGAGGTTTTTGCCGAGTTTGGCGATTGTGCCGACGGCGGATCTTTTGGAGAAGTCGGTGAAGGTGGAGGAGAAGAAGATTGTGGCGGAGATGAATCCGATGAGGTATAGGGGGAAACCCAGATTGGGAACTGTGGTGGAGCTTTTGAGAGTTACAGAGGATTTGAGCGGGAGGCTGGGGGATGTGAAGCTGCCGTTCATCGTCGTCCATGGCGATGCGGATGTTGTTACGGATCCGAATGTGAGCAAATCTCTGTATGAGGAAGCGCAGAGCGAGGACAAGACGATGAAGATTTATGAGGGAATGATGCATTCTATGCTGTTTGGGGAGACGGATGAGAATGTGGAGATTGTTCGAAATGATATTGTTTCTTGGTTGAATGCGAGATGTAGGAGTAGGATAAGTGATGATGATGATGATGATCAGAAGAATTAG

Coding sequence (CDS)

ATGTCCCAAACCCATCACCAAAATCCCCAATCCTTTCTGAACTACTGGGGAAACATCTCCGAGGAAGATTATTACGCTTCTCAACGAATCAAATCCTCCAAATCCTTCTACACTTCCCCTCGCGGCCTCAAGCTCTTCACTCGTTCATGGCTTCCTCTGCCTCCCACCCCTCCCCGAGCCCTCATCTTCATGGTCCATGGCTACGGCAACAACATCAGCTGGACCTTCCAAGCCACTGCCATCTTCCTCGCCCAGATGGGTTTCGCCTGCTTCGCTCTCGACCTCGAAGGCCATGGCGGATCCGAAGGTCTCAAAGCCTTCGTCCCTAATGTCGACTCTGTTGTCCACGACTGTCTCTCCTTCTTCCGATTCGTTAAATTGAACCCCCAATTTCAGGGTCTGCCCGCCTTCCTCTTCGGCGAGTCAATGGGCGGTGCGATTTGCTTATTGATTCATTTCGCTGATCCCAAAGGGTTCGAGGGCGCTATTCTCGTCGCACCCATGTGCAAAATCTCTGATAAAGTGAAACCCATTTGGCCGATTCCTCAATTTCTTACGGCGGTTGCGAGGTTTTTGCCGAGTTTGGCGATTGTGCCGACGGCGGATCTTTTGGAGAAGTCGGTGAAGGTGGAGGAGAAGAAGATTGTGGCGGAGATGAATCCGATGAGGTATAGGGGGAAACCCAGATTGGGAACTGTGGTGGAGCTTTTGAGAGTTACAGAGGATTTGAGCGGGAGGCTGGGGGATGTGAAGCTGCCGTTCATCGTCGTCCATGGCGATGCGGATGTTGTTACGGATCCGAATGTGAGCAAATCTCTGTATGAGGAAGCGCAGAGCGAGGACAAGACGATGAAGATTTATGAGGGAATGATGCATTCTATGCTGTTTGGGGAGACGGATGAGAATGTGGAGATTGTTCGAAATGATATTGTTTCTTGGTTGAATGCGAGATGTAGGAGTAGGATAAGTGATGATGATGATGATGATCAGAAGAATTAG

Protein sequence

MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLNARCRSRISDDDDDDQKN

Homology

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BLAST of Cp4.1LG09g08540 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.9e-65
Identity = 128/307 (41.69%), Postives = 194/307 (63.19%), Query Frame = 0


Query: 14  NYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVHGYGNNIS 73
           N+WG + EE+YY SQ +++SKS++ +P G KLFT+S+LPL     +  ++M HGYG++ S
Sbjct: 21  NFWGTMPEEEYYTSQGVRNSKSYFETPNG-KLFTQSFLPL-DGEIKGTVYMSHGYGSDTS 80

Query: 74  WTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQG 133
           W FQ   +  +  G+A FA DL GHG S+G++ ++ +++ V    L+FF+ V+ +  ++ 
Sbjct: 81  WMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSDPYKD 140

Query: 134 LPAFLFGESMGGAICLLIHF-ADPKGFEGAILVAPMCKISDKVKPIWPIPQFLTAVARFL 193
           LPAFLFGESMGG + LL++F ++P+ + G +  AP+  I + +KP          +    
Sbjct: 141 LPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLA 200

Query: 194 PSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGRLGDVKL 253
            + A +P   ++ K++K  EK  +   NP RY GKPR+GT+ ELLR T+ +    G V +
Sbjct: 201 DTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYVQENFGKVTI 260

Query: 254 PFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVS 313
           P    HG AD VT P  SK LYE+A S DKT+KIYEGM HS++ GE DEN EIV  D+  
Sbjct: 261 PVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMRE 320

Query: 314 WLNARCR 320
           W++ + +
Sbjct: 321 WIDEKVK 325

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BLAST of Cp4.1LG09g08540 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 2.5e-24
Identity = 90/280 (32.14%), Postives = 136/280 (48.57%), Query Frame = 0


Query: 42  GLKLFTRSWLPLPPTPPRALIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGS 101
           G  LF R W   P   P+ALIF+ HG G + S  ++  A  L  +    FA D  GHG S
Sbjct: 27  GQYLFCRYW--KPTGTPKALIFVSHGAGEH-SGRYEELARMLMGLDLLVFAHDHVGHGQS 86

Query: 102 EGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEG 161
           EG +  V +    V D L       +   + GLP FL G SMGGAI +L     P  F G
Sbjct: 87  EGERMVVSDFHVFVRDVLQ--HVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAG 146

Query: 162 AILVAPMCKISDKVKPIWPI--PQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEM 221
            +L++P+   + +    + +   + L  V   LP+L++ P    ++ SV    K  V   
Sbjct: 147 MVLISPLVLANPESATTFKVLAAKVLNLV---LPNLSLGP----IDSSVLSRNKTEVDIY 206

Query: 222 N--PMRYRGKPRLGTVVELLRVTEDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEA 281
           N  P+  R   ++   ++LL     +   L  + +PF+++ G AD + D   +  L E A
Sbjct: 207 NSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELA 266

Query: 282 QSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLNAR 318
           +S+DKT+KIYEG  H +L  E  E    V ++I  W++ R
Sbjct: 267 KSQDKTLKIYEGAYH-VLHKELPEVTNSVFHEINMWVSQR 293

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 4.0e-22
Identity = 82/278 (29.50%), Postives = 130/278 (46.76%), Query Frame = 0


Query: 42  GLKLFTRSWLPLPPTPPRALIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGS 101
           G  LF R W   P   P+ALIF+ HG G +    +   A  L  +    FA D  GHG S
Sbjct: 27  GQYLFCRYW--KPSGTPKALIFVSHGAGEHCG-RYDELAHMLKGLDMLVFAHDHVGHGQS 86

Query: 102 EGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEG 161
           EG +  V +    V D L     ++    +  +P FL G SMGGAI +L+    P  F G
Sbjct: 87  EGERMVVSDFQVFVRDVLQHVDTIQ--KDYPDVPIFLLGHSMGGAISILVAAERPTYFSG 146

Query: 162 AILVAPMCKISDKVKPIWPIPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMN- 221
            +L++P+   + +      +       A+ L  +    T   ++ SV    K  V   N 
Sbjct: 147 MVLISPLVLANPESASTLKV-----LAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNS 206

Query: 222 -PMRYRGKPRLGTVVELLRVTEDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQS 281
            P+  R   ++   ++LL     +   +  + LPF+++ G AD + D   +  L E ++S
Sbjct: 207 DPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRS 266

Query: 282 EDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLNAR 318
           +DKT+K+YEG  H +L  E  E    V +++ SW++ R
Sbjct: 267 QDKTLKMYEGAYH-VLHRELPEVTNSVLHEVNSWVSHR 293

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-21
Identity = 85/282 (30.14%), Postives = 130/282 (46.10%), Query Frame = 0


Query: 42  GLKLFTRSWLPLPPTPPRALIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGS 101
           G  LF R W   P   P+ALIF+ HG G +    +   A  L ++    FA D  GHG S
Sbjct: 27  GQYLFCRYW--KPSGTPKALIFVSHGAGEHCG-RYDELAQMLKRLDMLVFAHDHVGHGQS 86

Query: 102 EGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEG 161
           EG +  V +    V D L     V+    +  +P FL G SMGGAI +L     P  F G
Sbjct: 87  EGERMVVSDFQVFVRDLLQHVNTVQ--KDYPEVPVFLLGHSMGGAISILAAAERPTHFSG 146

Query: 162 AILVAPMCKISDKVKPIWPIPQFLTAVARFLPSLA--IVPTADL--LEKSVKVEEKKIVA 221
            IL++P+         I   P+  + +      L   ++P   L  ++ SV    K  V 
Sbjct: 147 MILISPL---------ILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEVD 206

Query: 222 EMN--PMRYRGKPRLGTVVELLRVTEDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYE 281
             N  P+      ++   ++LL     +   +  + LPF+++ G AD + D   +  L E
Sbjct: 207 LYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLME 266

Query: 282 EAQSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLNAR 318
            + S+DKT+K+YEG  H +L  E  E    V ++I +W++ R
Sbjct: 267 SSPSQDKTLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHR 293

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy Swiss-Prot
Match: P28321 (Monoglyceride lipase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJU3 PE=1 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 2.6e-13
Identity = 72/277 (25.99%), Postives = 119/277 (42.96%), Query Frame = 0


Query: 59  RALIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHG-GSEGLKAFVPNVDSVVHD 118
           R  + ++HG+G      F+     L+  G+  F  D  G G  S G    V +   V +D
Sbjct: 40  RGRVLLIHGFGEYTKIQFRLMD-HLSLNGYESFTFDQRGAGVTSPGRSKGVTDEYHVFND 99

Query: 119 CLSFFRFVKLNPQFQGLPAFLFGESMGGAICLLI-----HFADPKGFEGA---ILVAPMC 178
              F        + +G+P F++G SMGG ICL       H  +  G+ G+   I++ P  
Sbjct: 100 LEHFVEKNLSECKAKGIPLFMWGHSMGGGICLNYACQGKHKNEISGYIGSGPLIILHPHT 159

Query: 179 KISDKVKPIWPIPQFLTAVARFLPSLAIVPTADLLEKSVKVEEK-KIVAEMNPMRYRGKP 238
             +   + I P+      +A+FLP + I    DL  K +  ++  +     +PM     P
Sbjct: 160 MYNKPTQIIAPL------LAKFLPRVRIDTGLDL--KGITSDKAYRAFLGSDPM---SVP 219

Query: 239 RLGTV----------VELLRVTEDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQ 298
             G+            +L +   +   +      P I++HG  D + DP  S+   ++  
Sbjct: 220 LYGSFRQIHDFMQRGAKLYKNENNYIQKNFAKDKPVIIMHGQDDTINDPKGSEKFIQDCP 279

Query: 299 SEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLN 316
           S DK +K+Y G  HS+   ETD+    V ND+  WL+
Sbjct: 280 SADKELKLYPGARHSIFSLETDKVFNTVFNDMKQWLD 304

[Close]

BLAST of Cp4.1LG09g08540 vs. NCBI nr
Match: XP_023542714.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672 bits (1733), Expect = 1.15e-243
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA
Sbjct: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRISDDDDDDQKN 332
           ENVEIVRNDIVSWLNARCRSRISDDDDDDQKN
Sbjct: 301 ENVEIVRNDIVSWLNARCRSRISDDDDDDQKN 332

[Close]

BLAST of Cp4.1LG09g08540 vs. NCBI nr
Match: KAG7012415.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659 bits (1699), Expect = 1.76e-238
Identity = 326/332 (98.19%), Postives = 328/332 (98.80%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPP  PRA
Sbjct: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPALPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGF+GAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFDGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDV+LPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVRLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRISDDDDDDQKN 332
           ENVEIVRNDIVSWLNARCR RIS DDDDDQKN
Sbjct: 301 ENVEIVRNDIVSWLNARCRGRISVDDDDDQKN 332

[Close]

BLAST of Cp4.1LG09g08540 vs. NCBI nr
Match: KAG6573245.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 657 bits (1694), Expect = 1.02e-237
Identity = 325/332 (97.89%), Postives = 327/332 (98.49%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPP  PRA
Sbjct: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPALPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGF+GAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFDGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDV+LPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVRLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRISDDDDDDQKN 332
           ENVEIVRNDIVSWLNARCR RIS DDDDD KN
Sbjct: 301 ENVEIVRNDIVSWLNARCRGRISVDDDDDHKN 332

[Close]

BLAST of Cp4.1LG09g08540 vs. NCBI nr
Match: XP_022954754.1 (caffeoylshikimate esterase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 651 bits (1680), Expect = 1.49e-235
Identity = 322/327 (98.47%), Postives = 324/327 (99.08%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPP  PRA
Sbjct: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPALPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGF+GAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFDGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDV+LPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVRLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRISDDDD 327
           ENVEIVRNDIVSWLNARCRSRIS DDD
Sbjct: 301 ENVEIVRNDIVSWLNARCRSRISVDDD 327

[Close]

BLAST of Cp4.1LG09g08540 vs. NCBI nr
Match: XP_022954755.1 (caffeoylshikimate esterase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 647 bits (1669), Expect = 5.07e-234
Identity = 319/323 (98.76%), Postives = 321/323 (99.38%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPP  PRA
Sbjct: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPALPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGF+GAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFDGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDV+LPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVRLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRIS 323
           ENVEIVRNDIVSWLNARCRSRIS
Sbjct: 301 ENVEIVRNDIVSWLNARCRSRIS 323

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy TrEMBL
Match: A0A6J1GTY8 (caffeoylshikimate esterase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456913 PE=4 SV=1)

HSP 1 Score: 651 bits (1680), Expect = 7.23e-236
Identity = 322/327 (98.47%), Postives = 324/327 (99.08%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPP  PRA
Sbjct: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPALPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGF+GAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFDGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDV+LPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVRLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRISDDDD 327
           ENVEIVRNDIVSWLNARCRSRIS DDD
Sbjct: 301 ENVEIVRNDIVSWLNARCRSRISVDDD 327

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy TrEMBL
Match: A0A6J1GTA9 (caffeoylshikimate esterase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456913 PE=4 SV=1)

HSP 1 Score: 647 bits (1669), Expect = 2.45e-234
Identity = 319/323 (98.76%), Postives = 321/323 (99.38%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPP  PRA
Sbjct: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPALPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGF+GAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFDGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDV+LPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVRLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRIS 323
           ENVEIVRNDIVSWLNARCRSRIS
Sbjct: 301 ENVEIVRNDIVSWLNARCRSRIS 323

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy TrEMBL
Match: A0A6J1K547 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111490147 PE=4 SV=1)

HSP 1 Score: 639 bits (1649), Expect = 2.75e-231
Identity = 315/323 (97.52%), Postives = 319/323 (98.76%), Query Frame = 0


Query: 1   MSQTHHQNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRA 60
           MSQ HHQNPQ FLNYWGNISEED+YASQRIKSSKSFYTSPRGLKLFTRSWLPLPP  PRA
Sbjct: 1   MSQNHHQNPQFFLNYWGNISEEDHYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPVLPRA 60

Query: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120
           LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS
Sbjct: 61  LIFMVHGYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLS 120

Query: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWP 180
           FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADP+GF+GAILVAPMCKISDKVKPIWP
Sbjct: 121 FFRFVKLNPQFQGLPAFLFGESMGGAICLLIHFADPEGFDGAILVAPMCKISDKVKPIWP 180

Query: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240
           IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT
Sbjct: 181 IPQFLTAVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVT 240

Query: 241 EDLSGRLGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300
           EDLSGRLGDV+LPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD
Sbjct: 241 EDLSGRLGDVRLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETD 300

Query: 301 ENVEIVRNDIVSWLNARCRSRIS 323
           ENVEIVRNDIVSWLNARCRSRIS
Sbjct: 301 ENVEIVRNDIVSWLNARCRSRIS 323

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy TrEMBL
Match: A0A0A0LUH8 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073040 PE=4 SV=1)

HSP 1 Score: 581 bits (1498), Expect = 2.26e-208
Identity = 279/314 (88.85%), Postives = 302/314 (96.18%), Query Frame = 0


Query: 7   QNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVH 66
           ++PQSFLNYWGNISEEDY+ SQRIK+SKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVH
Sbjct: 5   EHPQSFLNYWGNISEEDYFTSQRIKASKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVH 64

Query: 67  GYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVK 126
           GYGNNISWTFQAT+IFLAQMGFACFALDLEGHG S+GLKAFVPNVDSVVHDCLSFF F+K
Sbjct: 65  GYGNNISWTFQATSIFLAQMGFACFALDLEGHGRSQGLKAFVPNVDSVVHDCLSFFNFLK 124

Query: 127 LNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLT 186
           L+PQFQGLP FL+GESMGGAICL+IHFADPKGF+GA+LVAPMCKISD VKP WPIPQFLT
Sbjct: 125 LDPQFQGLPCFLYGESMGGAICLMIHFADPKGFDGAVLVAPMCKISDNVKPHWPIPQFLT 184

Query: 187 AVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGR 246
           AVA+FLP+LAIVPTADLL+KSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTE LS R
Sbjct: 185 AVAQFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEHLSQR 244

Query: 247 LGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIV 306
           L DV LPFIV+HG+ADVVTDPNVSK+LYEEA+SEDKT+KIYEGMMHSML+GETDENVEIV
Sbjct: 245 LKDVNLPFIVLHGNADVVTDPNVSKTLYEEAKSEDKTIKIYEGMMHSMLYGETDENVEIV 304

Query: 307 RNDIVSWLNARCRS 320
           RNDI+ WLN RCR+
Sbjct: 305 RNDILCWLNERCRT 318

[Close]

BLAST of Cp4.1LG09g08540 vs. ExPASy TrEMBL
Match: A0A5D3DS62 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00460 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 5.32e-207
Identity = 277/314 (88.22%), Postives = 301/314 (95.86%), Query Frame = 0


Query: 7   QNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVH 66
           ++PQSFLNYWGNI EEDY+ SQRIK+SKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVH
Sbjct: 5   EHPQSFLNYWGNIPEEDYFTSQRIKASKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVH 64

Query: 67  GYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVK 126
           GYGNNISWTFQAT+IFLAQMGFACFALDLEGHG S+GLKAFVPNVDSVVHDCLSFF F+K
Sbjct: 65  GYGNNISWTFQATSIFLAQMGFACFALDLEGHGRSQGLKAFVPNVDSVVHDCLSFFNFLK 124

Query: 127 LNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLT 186
           L+PQFQGLP FL+GESMGGAICL+IHFADPKGF+GA+LVAPMCKISD VKP WPIPQFLT
Sbjct: 125 LDPQFQGLPCFLYGESMGGAICLMIHFADPKGFDGAVLVAPMCKISDNVKPHWPIPQFLT 184

Query: 187 AVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGR 246
           AVA+FLP+LAIVPTADLL+KSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTE LS R
Sbjct: 185 AVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEHLSQR 244

Query: 247 LGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIV 306
           L DV LPFIV+HG+ADVVTDP+VSK+LYEEA+SEDKT+KIYEGMMHSML+GETDENVEIV
Sbjct: 245 LKDVSLPFIVLHGNADVVTDPDVSKTLYEEAKSEDKTIKIYEGMMHSMLYGETDENVEIV 304

Query: 307 RNDIVSWLNARCRS 320
           RNDI+ WLN RCR+
Sbjct: 305 RNDILRWLNERCRT 318

[Close]

BLAST of Cp4.1LG09g08540 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 469.9 bits (1208), Expect = 1.7e-132
Identity = 221/312 (70.83%), Postives = 263/312 (84.29%), Query Frame = 0


Query: 7   QNPQSFLNYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVH 66
           Q  ++ +N+WG  SEEDY+  + I  SKSF+TSPRGL LFTRSWLP   +PPR LIFMVH
Sbjct: 4   QQLETEINFWGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVH 63

Query: 67  GYGNNISWTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVK 126
           GYGN++SWTFQ+T IFLAQMGFACFALD+EGHG S+G++A+VP+VD VV D +SFF  +K
Sbjct: 64  GYGNDVSWTFQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIK 123

Query: 127 LNPQFQGLPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLT 186
            NP+FQGLP FLFGESMGGAICLLI FADP GF+GA+LVAPMCKISDKV+P WP+ QFL 
Sbjct: 124 QNPKFQGLPRFLFGESMGGAICLLIQFADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLI 183

Query: 187 AVARFLPSLAIVPTADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGR 246
            ++RFLP+ AIVPT DLLEKS+KVEEKK +A+ NPMRY  KPRLGTV+ELLRVT+ L  +
Sbjct: 184 MISRFLPTWAIVPTEDLLEKSIKVEEKKPIAKRNPMRYNEKPRLGTVMELLRVTDYLGKK 243

Query: 247 LGDVKLPFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIV 306
           L DV +PFI+VHG AD VTDP VS+ LYE A+S+DKT+KIY+GMMHSMLFGE D+N+EIV
Sbjct: 244 LKDVSIPFIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIV 303

Query: 307 RNDIVSWLNARC 319
           R DIVSWLN RC
Sbjct: 304 RKDIVSWLNDRC 315

[Close]

BLAST of Cp4.1LG09g08540 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 4.0e-70
Identity = 133/291 (45.70%), Postives = 187/291 (64.26%), Query Frame = 0


Query: 27  SQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVHGYGNNISWTFQATAIFLAQM 86
           ++ IK  +SF  + RG+KLFT  W+P     P+AL+F+ HGY    S T  +TA  L + 
Sbjct: 5   TENIKYEESFIKNTRGMKLFTCKWVP-AKQEPKALVFICHGYAMECSITMNSTARRLVKA 64

Query: 87  GFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQGLPAFLFGESMGGA 146
           GFA + +D EGHG S+GL A+VPN D +V D  + +  +    + +G   FL GESMGGA
Sbjct: 65  GFAVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGA 124

Query: 147 ICLLIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLTAVARFLPSLAIVPTADLLEK 206
           + LL+H   P+ ++GA+LVAPMCKI++++KP   +   L  ++  +PS  I+P  D++E 
Sbjct: 125 VLLLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIET 184

Query: 207 SVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGRLGDVKLPFIVVHGDADVVTD 266
           + K  E +     NP  Y+G+PRL T  ELLRV+ DL  RL +V LPFIV+HG+ D VTD
Sbjct: 185 AFKQPEIRKQVRENPYCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTD 244

Query: 267 PNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLNAR 318
             VS+ LYE A S DKT K+Y GM H +L+GET EN+E V  DI+ WL+ +
Sbjct: 245 KAVSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWLDKK 294

[Close]

BLAST of Cp4.1LG09g08540 vs. TAIR 10
Match: AT5G16120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 6.9e-70
Identity = 128/286 (44.76%), Postives = 187/286 (65.38%), Query Frame = 0


Query: 30  IKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVHGYGNNISWTFQATAIFLAQMGFA 89
           IK+ +SF  + RG+++F++SWLP   + PRAL+   HGYG+  ++ F+  A  LA  G+ 
Sbjct: 53  IKTKESFEVNSRGVEIFSKSWLP-EASKPRALVCFCHGYGDTCTFFFEGIARRLALSGYG 112

Query: 90  CFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQGLPAFLFGESMGGAICL 149
            FA+D  G G SEGL  ++P+ D +V D +  +  +K NP+F  LP+FLFG+SMGGA+ L
Sbjct: 113 VFAMDYPGFGLSEGLHGYIPSFDLLVQDVIEHYSNIKANPEFSSLPSFLFGQSMGGAVSL 172

Query: 150 LIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLTAVARFLPSLAIVPTADLLEKSVK 209
            IH   P  + GA+L+APMCKI+D + P   + Q L  +A  LP   +VP  DL E   +
Sbjct: 173 KIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPKHKLVPQKDLAEAGFR 232

Query: 210 VEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGRLGDVKLPFIVVHGDADVVTDPNV 269
              K+ +   N + Y GKPRL T VE+LR T+D+  +L +V LP +++HG+AD VTDP+V
Sbjct: 233 DIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQEVSLPILILHGEADTVTDPSV 292

Query: 270 SKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLN 316
           S+ LYE+A+S DK + +YE   HS+L GE D+ +  V +DI+SWLN
Sbjct: 293 SRELYEKAKSPDKKIVLYENAYHSLLEGEPDDMILRVLSDIISWLN 337

[Close]

BLAST of Cp4.1LG09g08540 vs. TAIR 10
Match: AT5G16120.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 6.9e-70
Identity = 128/286 (44.76%), Postives = 187/286 (65.38%), Query Frame = 0


Query: 30  IKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVHGYGNNISWTFQATAIFLAQMGFA 89
           IK+ +SF  + RG+++F++SWLP   + PRAL+   HGYG+  ++ F+  A  LA  G+ 
Sbjct: 71  IKTKESFEVNSRGVEIFSKSWLP-EASKPRALVCFCHGYGDTCTFFFEGIARRLALSGYG 130

Query: 90  CFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQGLPAFLFGESMGGAICL 149
            FA+D  G G SEGL  ++P+ D +V D +  +  +K NP+F  LP+FLFG+SMGGA+ L
Sbjct: 131 VFAMDYPGFGLSEGLHGYIPSFDLLVQDVIEHYSNIKANPEFSSLPSFLFGQSMGGAVSL 190

Query: 150 LIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLTAVARFLPSLAIVPTADLLEKSVK 209
            IH   P  + GA+L+APMCKI+D + P   + Q L  +A  LP   +VP  DL E   +
Sbjct: 191 KIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPKHKLVPQKDLAEAGFR 250

Query: 210 VEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGRLGDVKLPFIVVHGDADVVTDPNV 269
              K+ +   N + Y GKPRL T VE+LR T+D+  +L +V LP +++HG+AD VTDP+V
Sbjct: 251 DIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQEVSLPILILHGEADTVTDPSV 310

Query: 270 SKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVSWLN 316
           S+ LYE+A+S DK + +YE   HS+L GE D+ +  V +DI+SWLN
Sbjct: 311 SRELYEKAKSPDKKIVLYENAYHSLLEGEPDDMILRVLSDIISWLN 355

[Close]

BLAST of Cp4.1LG09g08540 vs. TAIR 10
Match: AT5G19290.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 9.9e-69
Identity = 131/309 (42.39%), Postives = 190/309 (61.49%), Query Frame = 0


Query: 14  NYWGNISEEDYYASQRIKSSKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMVHGYGNNIS 73
           N +G+++ +++YA   +  S +F T+PRGLKLFT+ W PLPPT P  +I +VHG+    S
Sbjct: 13  NPFGSLTADEFYAKHSVSHSSAFITNPRGLKLFTQWWSPLPPTKPIGIIAVVHGFTGESS 72

Query: 74  WTFQATAIFLAQMGFACFALDLEGHGGSEGLKAFVPNVDSVVHDCLSFFRFVKLNPQFQG 133
           W  Q T+I  A+ GF   A+D +GHG S+GL A +P+++ VV DC+SFF   +       
Sbjct: 73  WFLQLTSILFAKSGFITCAIDHQGHGFSDGLIAHIPDINPVVDDCISFFDDFRSRQTPSD 132

Query: 134 LPAFLFGESMGGAICLLIHFADPKGFEGAILVAPMCKISDKVKPIWPIPQFLTAVARFLP 193
           LP FL+ ES+GGAI L I       ++G IL   MC ISDK KP WP+   L  VA  +P
Sbjct: 133 LPCFLYSESLGGAIALYISLRQRGVWDGLILNGAMCGISDKFKPPWPLEHLLFVVANLIP 192

Query: 194 SLAIVPT-ADLLEKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEDLSGRLGDVKL 253
           +  ++PT   + + S K   K+ +A  +P R   KPR  T  EL+RV +DL GR  +V++
Sbjct: 193 TWRVIPTRGSIPDVSFKEPWKRKLAMASPRRTVAKPRAATAYELIRVCKDLQGRFEEVEV 252

Query: 254 PFIVVHGDADVVTDPNVSKSLYEEAQSEDKTMKIYEGMMHSMLFGETDENVEIVRNDIVS 313
           P ++VHG  DVV D    + L+  A SEDKT+KIY  + H M+ GE++E V++V  D++S
Sbjct: 253 PLLIVHGGGDVVCDVACVEELHRRAISEDKTIKIYPELWHQMI-GESEEKVDLVYGDMLS 312

Query: 314 WLNARCRSR 322
           WL +R   +
Sbjct: 313 WLKSRAERK 320

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g08540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C942	2.9e-65	41.69	Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1	[more]
Q99685	2.5e-24	32.14	Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2	[more]
O35678	4.0e-22	29.50	Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1	[more]
Q8R431	2.6e-21	30.14	Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1	[more]
P28321	2.6e-13	25.99	Monoglyceride lipase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX...	[more]

BLAST of Cp4.1LG09g08540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023542714.1	1.15e-243	100.00	caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7012415.1	1.76e-238	98.19	Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6573245.1	1.02e-237	97.89	Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022954754.1	1.49e-235	98.47	caffeoylshikimate esterase-like isoform X1 [Cucurbita moschata]	[more]
XP_022954755.1	5.07e-234	98.76	caffeoylshikimate esterase-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Cp4.1LG09g08540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GTY8	7.23e-236	98.47	caffeoylshikimate esterase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1GTA9	2.45e-234	98.76	caffeoylshikimate esterase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1K547	2.75e-231	97.52	caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111490147 PE=4...	[more]
A0A0A0LUH8	2.26e-208	88.85	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073040...	[more]
A0A5D3DS62	5.32e-207	88.22	Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Cp4.1LG09g08540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11090.1	1.7e-132	70.83	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39420.1	4.0e-70	45.70	alpha/beta-Hydrolases superfamily protein	[more]
AT5G16120.1	6.9e-70	44.76	alpha/beta-Hydrolases superfamily protein	[more]
AT5G16120.2	6.9e-70	44.76	alpha/beta-Hydrolases superfamily protein	[more]
AT5G19290.1	9.9e-69	42.39	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 87..102 score: 34.48 coord: 138..151 score: 46.8 coord: 254..268 score: 27.36
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 21..324 e-value: 4.0E-56 score: 192.4
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 29..317
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 57..298 e-value: 2.6E-54 score: 184.0
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 18..317
None	No IPR available	PANTHER	PTHR11614:SF20	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 18..317

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g08540.1	Cp4.1LG09g08540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity