Cp4.1LG09g07090 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g07090
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprimase homolog protein-like isoform X1
LocationCp4.1LG09: 6371301 .. 6377176 (+)
RNA-Seq ExpressionCp4.1LG09g07090
SyntenyCp4.1LG09g07090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCTCTTTCTCCTACTCCGCCGTCGCCATCGCCACCACCAGGTACTTAATCGTTTCGACCTTGTTATAATACTCTTTTGCTTTGTTTACGGTTTTAGGGTTAATGTTATGGGCGAATCTAAAGTGAAGGTTCTAATGGAGAAAATGGATCTTATCGGAATAGTCTGTGATGAACATTGTACTCCTGGAAAATACTATTGCCTGCTCTGTCCCAAGGTTATCTGCTCCTCCCGTCCATGCCTTTCGTGGTTTCTATACATGAAAATCAAATCCATATTCTTGTTTTGATGGTTGAACAGTGCAAGGGTGGGCGCTTAATGGAGAGGAGCTTATCTCTACATGTAATTCCAACAGGGTAAGTGATTATTACTGTTTTTCCCTTGATTTTATGAGGATTGATTGGCAGTACATTGTTTATGAATTACATTTTGTTATTGGGAACAGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGGTAACGTCATATTAATATGTTCAATCTCTTATTTAACAATTAACACATGAGAATTTCTTAGCCGAATGATTGTTTGGTTTAGATATTTGCAGATGGCAGATTGGCGTTTAATGAATTCAGCAGAACTGCGAAGTTGTTTGGAGGAACTGTGAAGAAGAGTCTTATTTTGGAGCCTCTTGGTGATGAGGTACTTCGAATTTCATTGCATTTCTATGTATAAGACACACTTAGACTAATACGTTAGTTCTGAGACCCCACAATGGTTGGAGAGGAAATCTCTCCATAGTAGACGCGTTTTAAAACTGTAAGGCTGACGGCGATACGTAACGGGCTAAAAGGACAATGTTTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATTAGAGCTAGACACCAGGTGGTGTGCTAGTGAGGACATTGGGCTCCCAAGGAGGGTGGATTGTGAGATCTCACATCGGTTGAAGAGGAGAACGAAGCATTGCTTATAAGGGTGTGGAAACCTCTTCCTACCAGACGTGTTTTAAAAACCTTGAGGGGAAGCCTAGGAGGGATAGCCCAAGAGGACAATATTAGAGCCAGACACCAGGTGGTGTGCCAGTGAGGATACTGGGCCCCCAAGGGGGGTGGATTGTGAGATCTCACATCGGTTGGAGAGGAGAACGAAACATTGCTTATAAGGGTGTGAAAACCTCTCCCTACCAGATGCGATTTAAAAACCTTGAGGGATAGCCCAAAGAGGACAATATTTGCTAGTGGTGGAATTGGACTGTAACAAATGGTATCAGAGCCAGACAATAGGCAGTGTGCCAGCGAGGACGATGGGCCTCAAGGGGGGTAGATTGTGAGATCCCACATCGATTGGAGAGGAAAACGAAGTATTCCTTATAAGGGTATGGAAACCTTTCCCTAGTAGACGTGTTTTAAAATTGTAAGGCTGATGGTGATATAATGGGCCAAAATGAACAATATCTGCTAGGGGTGTGCTTGGGCTTGGGCTGATATTAGTATTGTTTGAATGGGTTAACTTGTGCTGTCAAGAATGGGTTAACTTGTGCAATCTTTTGACAGAACCACGGTTAATTTTGATTGCTAGGTAGTCCTACATGTTACGATGAATAAGACTAGGAAGACTTTTGATCTTAATGCAAACTGTTGAATTTATCTTTGAATTTGAGGTTCAAGTGTTTTGATGTTTCGTTTTCAGCTAATCACTTACTTCAGCAAGAGAATGATATCCCAGGAAACTCTCAAAAGGAATGTTGTTATGCAAACAGCTGATCGTCAGGTACCTGAAAGAATGCTTTTGTTTGTATGCCTTTCATTGAATTTTTAGACTCGTACCCTTGTGGATGCAATACTGCCCAGAAGTTATGTAACAGCCCAAGCCCAAGCCCAAGCTCACTGCTAGTTGATATTATCCTCTTTTGACTTTCCCTTTCGAGCTTCCTCTCAAGGTTTTTAAAACGTGTTTGCAAGGGAGAGCTTTCCACACCCATATAAAAATGTTTCGTTCTCCTCCACAATCAATGTGGGATCTCAATCCACCCCCCTTCGGGGCCCAGCGTCCTCGCTGCACTTGTTCCCTTCTCCAATCGATGTGGGACCCCCCAATCCACCATCTTTCGGGGCCCAACGTCCTTGCTGGTACACCACCTCGTGCACACCTCCTTCAGGGCTTAGCCTCTTCACTGACACATCGCCCAGTGTCTGGCTCTAATACCATTTGTAATAGCTCAAGCCCACCGCTAGCAAATATTGTCTTCTTTAGGTTTTCCTTTTCAGGCTTCCCCTCAAGGTTTTTTAAAACGTAGGGAGAGGTCTCCACACCCTTGTAAAAAATGCTTCGTTCTCCTCTCCAACCGATGTGGGATCTCACAAGCGCGTCTCTCTCATTTCCTTGTAGCTTCAACTTGTGACTAGTTTAAAGGTCACTTGCAGGCTGCAATTGCATTCACTTATCGACAAAACGGACAGCTCGTTGGCTGTAAGTACCGAACCATAAACAAGAAATTTTGGCAGGTACATTAATCAATCCTTCTTCAATCAGAGTTATTTTAGAAACTGTCCAGCATCTTCCTGTTCTTCGGCTTGTAATTCTAAGCGTTTCTAAGCTTTCTAATCTGTATGTCTGATATCAATATTCAGGCGAAGGGCACAGAAAAATTGTTTTATGGGATTGATGATATTAATGATGCAGATGAACTCATCATTGTATGAATTCACAATACCTTGTTTTGTAAGTTTGTATTTTATATTTTGATCTATTTTTCCATCGATTTTTGATAATGTTGTGTATGTCGATTAAGGTCGAAGGTGAAATGGATAAACTTTCAGTAGAAGAAGCTGGCTTCCTGAATTGTATCAGTGTTCCTGGTGGTGCCCCAACTAAGGTTTCAACTAATGCAGTAGCATCAATTCAAAAGGTTCTGTGTTATAGAAATTCATTTATGCCTATCTTGTTTGCTTTTACATCATTCCTAGCTATCTGAGTTATGCTACTAAACTATTTCCCTCTTCCCTCGATCCCACATTGGTTGGAGAGGGGAACGAATCATTCTTTATAAGGGTGTGGCAACCTTTCCCTAGCAGAAGCGTTTTAAAAGCTTGGGTTGTTACAAATGGTATTAGAGCCAGACACCGGGCGATGTGTCAGCGAAGAGGTTGAGCCCTGAAGGGGGGTGAAGCCAGCAAGGACGTTGGACCCCGAACTGGGTGGATTGGGGGGTCTCATATCGATTGGAGAAGGGAACGAGTGCCAGCGACGACGCTGGCCCCAAAGGGGGATGGATCGTGAGATCCCACATTGGAAACATTTTTTATAAGGGTGTGGAAACCTCTCCCTAACAGACGTTTTAAAATCTTGAGGGGAAGCCCAGAAAGAAAAACCCAAAAAGGACAATATCTGCTAGTGGTGGGCTTGAGCTGTTACATTACGGAACTTGAAATGTGGTTTAAAATGGAGTAAAACACACCTTTGATACTCAAAGATTTAAATTTAGTATCTATTTAGTCATTTAGTTCTTGAAATTTGAATACGTTTCTATGTGTTTTGAGTTTCATTTTTAATAGATCTCGAAAGTTTGGAATTGGTTTCTAATTGGTCCTTCAGCGTAAAAAGTTAAAAACTTCAAATTTTAGAGACCAATTAGAAATTGGTTTCTAATTGGTCCTTCAGCGTAAAAAGTTAAAAACTTCAAATTTTAGAGACCAATTAGAAACTAGATTCAAAATTCGAGGATGAGAGTTTTAGGATTTCAAGGACCAGATAGAAACTAAATCTCAATCTAAAAAACTAGACGTGTAATGGTTCATCTTTAGAAAGTTACGACAAGAAGGGACACTAAAGCATCGTATAAGAAACGCTCTACACCCGTTTTCAACTAGAAACCAAAGATAAGAAGAACTGATTCTAAGTTAAAACTTTTGTGATAATATTAGGTTTAGCTATGATGATCATATATATTTTACAATTTATATGGTTTCACATGTCAGGATACTGCATATCAGTATTTATGGAGCTGCAAAGACTTGTTAGATAAGGTAAATAATATGGTCTGGTCCTGGTTTATTCTGGATTTTGAAAGTTTTTTAAAAGACCAAATAGTTATCAAATGGAGCCTTCATTAACGAGATTTTGCTGCTTTTTTTCTTTTATGTTAGAAATAAGCACGTTAACAGCTTCGTCGTTTTGTAATTTACGTTTTGAAAACGTGTTTTAAAGTTTGAAATACTAACAAAATTATTAACCTGAAAGGTTCCCCGGATTATATTGGCTACTGATAGTGATGAACCAGGGCAAGCTTTGGCAGAAGAGCTAGCACTTCGGCTTGGAAAACACAGGTCTCCACTCAGTTGTGAACTTTGTGTTAATCTTAACGCTTGATTTGTTTTTCATAAAGGCAAAATAACGTGGCTTTGTTGTTTTGGTTAACAGATGTTGGCGGGTAGATTGGCCTTATAAAGATGACTTCAACCGTTTTAAAGATGCCAATGAGGTTACATTCCTCCCTAAATTACTCTTTTAAGTTATTGTTTTTAGCTCAACAAGGTGGGATGGTGCTGTGCCGTAACGTCAAACCTTAATGCTCTGGTACTAAGTACCAATGGTATGATACCACTTGTTGTACTTAAATGGCAAAATCAAAATGTTCTGATACTCAATTATTGTTGCTGAGGCGGAAGCAAGACCGGTCGCACTCACACACCCACAAACAGAAATTAAACAAAGAGACACAAAAATTTACGTGGTTTGAAGAGAAGAAATTTCCCTACATCCACGGGTGATAGTCAATCACTATAACCAAAGTGTTTACAAGTGTATCTCTCGCACTCTTACACTACAAAGTCTCAGAACAAAGTTCTTCTCAATTGCCTCTAAGCAAATACAAAATGACAATAACTCCACACTTCAAAAGAATGCTAGACAAGCGTTTATATAGGCATCATGAACCAAATAAAAGAGAATATGAACAAAAGGGTTATAAACTAAATGAGAGTTAAGAACCAGGTGAAGTCGAGCATCCTAAAGCTAATTATAGATCATTATTCTATGAAGTACGTTTTATATTCTTACTATTTTGGTTGAAAATCTGGAAAACGGCAAGGATGCCAAAAAGAGGGCGGTTTCGTGTCGTTAATTCGAAGCTTGTTTCTTGGGATGATGATATTTATTTTGATCCTTTTGGCATTTGAATGTGTTATTACAAACAGATTCTGATGCAGTTGGGCGCAGATGCTTTAAAGAAAGCAATTGAAGATGCCAAGTCATATCAACTTTGTGATACACATCAAATACAAGTAGGTGGGTATCCAAATATTTCCAGAAAATTCCCATTTTTAAAATGTTTTTGATTTGGGTTTTGGTAATTATTTTGATGACCTTCCTCTCTTTTAACTTTGCAGAAGAGGACCTCGAGAGCTTCCACGAGCCTTCGTAACTATGGAGAATCATTTTGAGTGGTAATCGGTAACAATTAGATTCATAAATTACATGGAAATAAACGTTTCGTTCGTGCAGTGAAAACATTGATATTGACTTTGATTTTACTTTGAATTAAAATAAAAACTAAAGTTTTTATATTAAAGAAATATGACTTCCCTCGATCTTTGTTTAACCACACGCTCTATGGATTAAACATTCGACCTTATGGTTAATAGTCACATGCTCTCTAACCAACTGAATTACCCTTTTACTATACATATACAACTATTAAAAAGTTTCTAAACTACTGCTAAAAGAAAAGCTTGAGCCTTCAACCTTACTTGACTTGACAAATCTAAGAGCCACTTGCTATCATCACATGACTTTGCCCATCTCGGATTTGGACTCTCAAGGTCAATTCAAGCCCACCAAATGTCCCCGATAAACACCCATGAT

mRNA sequence

ATGCGCTCTTTCTCCTACTCCGCCGTCGCCATCGCCACCACCAGGGTTAATGTTATGGGCGAATCTAAAGTGAAGGTTCTAATGGAGAAAATGGATCTTATCGGAATAGTCTGTGATGAACATTGTACTCCTGGAAAATACTATTGCCTGCTCTGTCCCAAGTGCAAGGGTGGGCGCTTAATGGAGAGGAGCTTATCTCTACATGTAATTCCAACAGGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGATATTTGCAGATGGCAGATTGGCGTTTAATGAATTCAGCAGAACTGCGAAGTTGTTTGGAGGAACTGTGAAGAAGAGTCTTATTTTGGAGCCTCTTGGTGATGAGCTAATCACTTACTTCAGCAAGAGAATGATATCCCAGGAAACTCTCAAAAGGAATGTTGTTATGCAAACAGCTGATCGTCAGGCTGCAATTGCATTCACTTATCGACAAAACGGACAGCTCGTTGGCTGTAAGTACCGAACCATAAACAAGAAATTTTGGCAGGCGAAGGGCACAGAAAAATTGTTTTATGGGATTGATGATATTAATGATGCAGATGAACTCATCATTGTCGAAGGTGAAATGGATAAACTTTCAGTAGAAGAAGCTGGCTTCCTGAATTGTATCAGTGTTCCTGGTGGTGCCCCAACTAAGGTTTCAACTAATGCAGTAGCATCAATTCAAAAGGATACTGCATATCAGTATTTATGGAGCTGCAAAGACTTGTTAGATAAGGTTCCCCGGATTATATTGGCTACTGATAGTGATGAACCAGGGCAAGCTTTGGCAGAAGAGCTAGCACTTCGGCTTGGAAAACACAGATGTTGGCGGGTAGATTGGCCTTATAAAGATGACTTCAACCGTTTTAAAGATGCCAATGAGATTCTGATGCAGTTGGGCGCAGATGCTTTAAAGAAAGCAATTGAAGATGCCAAGTCATATCAACTTTGTGATACACATCAAATACAAGTAGAAGAGGACCTCGAGAGCTTCCACGAGCCTTCGTAACTATGGAGAATCATTTTGAGTGGTAATCGGTAACAATTAGATTCATAAATTACATGGAAATAAACGTTTCGTTCGTGCAGTGAAAACATTGATATTGACTTTGATTTTACTTTGAATTAAAATAAAAACTAAAGTTTTTATATTAAAGAAATATGACTTCCCTCGATCTTTGTTTAACCACACGCTCTATGGATTAAACATTCGACCTTATGGTTAATAGTCACATGCTCTCTAACCAACTGAATTACCCTTTTACTATACATATACAACTATTAAAAAGTTTCTAAACTACTGCTAAAAGAAAAGCTTGAGCCTTCAACCTTACTTGACTTGACAAATCTAAGAGCCACTTGCTATCATCACATGACTTTGCCCATCTCGGATTTGGACTCTCAAGGTCAATTCAAGCCCACCAAATGTCCCCGATAAACACCCATGAT

Coding sequence (CDS)

ATGCGCTCTTTCTCCTACTCCGCCGTCGCCATCGCCACCACCAGGGTTAATGTTATGGGCGAATCTAAAGTGAAGGTTCTAATGGAGAAAATGGATCTTATCGGAATAGTCTGTGATGAACATTGTACTCCTGGAAAATACTATTGCCTGCTCTGTCCCAAGTGCAAGGGTGGGCGCTTAATGGAGAGGAGCTTATCTCTACATGTAATTCCAACAGGAGATTTTGCAATGTGGAGGTGTTTCCAGTCTGAGTGTGGTTGGGCAGGCCGGATATTTGCAGATGGCAGATTGGCGTTTAATGAATTCAGCAGAACTGCGAAGTTGTTTGGAGGAACTGTGAAGAAGAGTCTTATTTTGGAGCCTCTTGGTGATGAGCTAATCACTTACTTCAGCAAGAGAATGATATCCCAGGAAACTCTCAAAAGGAATGTTGTTATGCAAACAGCTGATCGTCAGGCTGCAATTGCATTCACTTATCGACAAAACGGACAGCTCGTTGGCTGTAAGTACCGAACCATAAACAAGAAATTTTGGCAGGCGAAGGGCACAGAAAAATTGTTTTATGGGATTGATGATATTAATGATGCAGATGAACTCATCATTGTCGAAGGTGAAATGGATAAACTTTCAGTAGAAGAAGCTGGCTTCCTGAATTGTATCAGTGTTCCTGGTGGTGCCCCAACTAAGGTTTCAACTAATGCAGTAGCATCAATTCAAAAGGATACTGCATATCAGTATTTATGGAGCTGCAAAGACTTGTTAGATAAGGTTCCCCGGATTATATTGGCTACTGATAGTGATGAACCAGGGCAAGCTTTGGCAGAAGAGCTAGCACTTCGGCTTGGAAAACACAGATGTTGGCGGGTAGATTGGCCTTATAAAGATGACTTCAACCGTTTTAAAGATGCCAATGAGATTCTGATGCAGTTGGGCGCAGATGCTTTAAAGAAAGCAATTGAAGATGCCAAGTCATATCAACTTTGTGATACACATCAAATACAAGTAGAAGAGGACCTCGAGAGCTTCCACGAGCCTTCGTAA

Protein sequence

MRSFSYSAVAIATTRVNVMGESKVKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAKSYQLCDTHQIQVEEDLESFHEPS
Homology
BLAST of Cp4.1LG09g07090 vs. ExPASy Swiss-Prot
Match: B5X582 (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.2e-83
Identity = 157/330 (47.58%), Postives = 214/330 (64.85%), Query Frame = 0

Query: 5   SYSAVAIATTRVNVMGESKVKVLMEKMDLIGI------VCDEHCTPGKYYCLLCPKCKGG 64
           SY+++    T V+   E+  +V++ ++  +        V  E+C PG++  L+CP C+GG
Sbjct: 83  SYNSIPRVPTPVDTEVEADKRVVLSRLVTLRRKLAEQGVDAENCPPGQHSGLICPTCEGG 142

Query: 65  RLMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLI 124
              E+SLSL + P G  A W CF+ +CG  G + ADG LA        K+      + + 
Sbjct: 143 NSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADGGLA--SADPIEKVERKITVEGIE 202

Query: 125 LEPLGDELITYFSKRMISQETLKRNVVMQ-TADRQAAIAFTYRQNGQLVGCKYRTINKKF 184
           LEPL DE+  YF+ R IS++TL+RN VMQ     +  IAFTY Q G+LV CKYR++ K F
Sbjct: 203 LEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFTYWQRGELVSCKYRSLTKMF 262

Query: 185 WQAKGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVAS 244
           +Q + T ++ YG+DDI    E+IIVEGE+DKL++EEAGFLNC+SVP GAP KVS+  + S
Sbjct: 263 FQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLNCVSVPDGAPAKVSSKEIPS 322

Query: 245 IQKDTAYQYLWSCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDF 304
             KDT Y++LW+C D L K  RI++ATD D PGQA+AEE+A RLGK RCWRV WP K + 
Sbjct: 323 EDKDTKYKFLWNCNDYLKKASRIVIATDGDGPGQAMAEEIARRLGKERCWRVKWPKKSED 382

Query: 305 NRFKDANEILMQLGADALKKAIEDAKSYQL 328
             FKDANE+LM  G   LK+AI DA+ Y +
Sbjct: 383 EHFKDANEVLMSKGPHLLKEAILDAEPYPI 410

BLAST of Cp4.1LG09g07090 vs. ExPASy Swiss-Prot
Match: F4I6E6 (Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 3.0e-81
Identity = 155/307 (50.49%), Postives = 203/307 (66.12%), Query Frame = 0

Query: 22  SKVKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCF 81
           SK+  LM K+   GI   ++C PG   CL+CPKC+ G   E+SL+L++ P G  A W C 
Sbjct: 33  SKLVTLMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC- 92

Query: 82  QSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLK 141
           + +CG  G +  DG+L   +     K+      +S+ LEPL DE+  +F+ R IS +TL+
Sbjct: 93  RRKCGLKGVLQVDGKLVSKD--PIGKVERKITVESIKLEPLCDEIQDFFAARAISGKTLE 152

Query: 142 RNVVMQ-TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELI 201
           RN VMQ   D +  IAFTY Q G+LV CKYR++ KKF Q + T K+ YG+DDI +  E+I
Sbjct: 153 RNRVMQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEII 212

Query: 202 IVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRI 261
           IVEGE DKL++EEAGF NC+SVP GAP  VS+  + S  KDTA++Y+W+C D L K  RI
Sbjct: 213 IVEGEPDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRI 272

Query: 262 ILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIE 321
           ++ATD D PGQALAEELA RLGK RCW V WP K +   FKDANE+LM  G   LK+AI 
Sbjct: 273 VIATDGDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAIL 332

Query: 322 DAKSYQL 328
           +A+ Y L
Sbjct: 333 NAEPYPL 335

BLAST of Cp4.1LG09g07090 vs. NCBI nr
Match: XP_023541322.1 (primase homolog protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665 bits (1716), Expect = 9.33e-240
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 24  VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 83
           VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS
Sbjct: 76  VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 135

Query: 84  ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 143
           ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN
Sbjct: 136 ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 195

Query: 144 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 203
           VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE
Sbjct: 196 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 255

Query: 204 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 263
           GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA
Sbjct: 256 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 315

Query: 264 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 323
           TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK
Sbjct: 316 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 375

Query: 324 SYQLCDTHQIQVEEDLESFHEPS 346
           SYQLCDTHQIQVEEDLESFHEPS
Sbjct: 376 SYQLCDTHQIQVEEDLESFHEPS 398

BLAST of Cp4.1LG09g07090 vs. NCBI nr
Match: XP_022955059.1 (primase homolog protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 654 bits (1688), Expect = 1.48e-235
Identity = 318/323 (98.45%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 24  VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 83
           VKVLMEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS
Sbjct: 72  VKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 131

Query: 84  ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 143
           ECGWAGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELITYFSKRMISQETLKRN
Sbjct: 132 ECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRN 191

Query: 144 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 203
           VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE
Sbjct: 192 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 251

Query: 204 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 263
           GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA
Sbjct: 252 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 311

Query: 264 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 323
           TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+QLGADALKKAIEDAK
Sbjct: 312 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIEDAK 371

Query: 324 SYQLCDTHQIQVEEDLESFHEPS 346
           SYQLCDT +IQVEEDLESFHEPS
Sbjct: 372 SYQLCDTDEIQVEEDLESFHEPS 394

BLAST of Cp4.1LG09g07090 vs. NCBI nr
Match: KAG6573428.1 (Primase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 653 bits (1685), Expect = 4.58e-235
Identity = 317/323 (98.14%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 24  VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 83
           VKVLMEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS
Sbjct: 74  VKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 133

Query: 84  ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 143
           ECGWAGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELITYFSKRMISQETLKRN
Sbjct: 134 ECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRN 193

Query: 144 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 203
           VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE
Sbjct: 194 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 253

Query: 204 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 263
           GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA
Sbjct: 254 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 313

Query: 264 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 323
           TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+QLGADALKKAI+DAK
Sbjct: 314 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIQDAK 373

Query: 324 SYQLCDTHQIQVEEDLESFHEPS 346
           SYQLCDT +IQVEEDLESFHEPS
Sbjct: 374 SYQLCDTDEIQVEEDLESFHEPS 396

BLAST of Cp4.1LG09g07090 vs. NCBI nr
Match: XP_022955060.1 (primase homolog protein-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 649 bits (1673), Expect = 1.75e-234
Identity = 314/319 (98.43%), Postives = 316/319 (99.06%), Query Frame = 0

Query: 28  MEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 87
           MEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW
Sbjct: 1   MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 60

Query: 88  AGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 147
           AGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELITYFSKRMISQETLKRNVVMQ
Sbjct: 61  AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 120

Query: 148 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 207
           TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD
Sbjct: 121 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 180

Query: 208 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 267
           KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD
Sbjct: 181 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 240

Query: 268 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAKSYQL 327
           EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+QLGADALKKAIEDAKSYQL
Sbjct: 241 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIEDAKSYQL 300

Query: 328 CDTHQIQVEEDLESFHEPS 346
           CDT +IQVEEDLESFHEPS
Sbjct: 301 CDTDEIQVEEDLESFHEPS 319

BLAST of Cp4.1LG09g07090 vs. NCBI nr
Match: XP_022994814.1 (primase homolog protein isoform X1 [Cucurbita maxima])

HSP 1 Score: 649 bits (1674), Expect = 3.64e-233
Identity = 316/323 (97.83%), Postives = 317/323 (98.14%), Query Frame = 0

Query: 24  VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 83
           VKVLMEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS
Sbjct: 88  VKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 147

Query: 84  ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 143
           ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN
Sbjct: 148 ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 207

Query: 144 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 203
           VVMQTA RQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE
Sbjct: 208 VVMQTAGRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 267

Query: 204 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 263
           GEMDKLSVEEAGFLNCISVPGGAP KVSTNAVASIQKDTAYQYLWSCKDLLDKVPR+ILA
Sbjct: 268 GEMDKLSVEEAGFLNCISVPGGAPNKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRVILA 327

Query: 264 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 323
           TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK
Sbjct: 328 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 387

Query: 324 SYQLCDTHQIQVEEDLESFHEPS 346
           SYQLCDT  IQVE DLESFHEPS
Sbjct: 388 SYQLCDTDHIQVEGDLESFHEPS 410

BLAST of Cp4.1LG09g07090 vs. ExPASy TrEMBL
Match: A0A6J1GUU9 (primase homolog protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457136 PE=4 SV=1)

HSP 1 Score: 654 bits (1688), Expect = 7.18e-236
Identity = 318/323 (98.45%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 24  VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 83
           VKVLMEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS
Sbjct: 72  VKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 131

Query: 84  ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 143
           ECGWAGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELITYFSKRMISQETLKRN
Sbjct: 132 ECGWAGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRN 191

Query: 144 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 203
           VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE
Sbjct: 192 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 251

Query: 204 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 263
           GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA
Sbjct: 252 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 311

Query: 264 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 323
           TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+QLGADALKKAIEDAK
Sbjct: 312 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIEDAK 371

Query: 324 SYQLCDTHQIQVEEDLESFHEPS 346
           SYQLCDT +IQVEEDLESFHEPS
Sbjct: 372 SYQLCDTDEIQVEEDLESFHEPS 394

BLAST of Cp4.1LG09g07090 vs. ExPASy TrEMBL
Match: A0A6J1GU46 (primase homolog protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457136 PE=4 SV=1)

HSP 1 Score: 649 bits (1673), Expect = 8.47e-235
Identity = 314/319 (98.43%), Postives = 316/319 (99.06%), Query Frame = 0

Query: 28  MEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 87
           MEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW
Sbjct: 1   MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 60

Query: 88  AGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 147
           AGRIFADGRLAFNEFSRTAKLFGGT KKSLILEPLGDELITYFSKRMISQETLKRNVVMQ
Sbjct: 61  AGRIFADGRLAFNEFSRTAKLFGGTAKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 120

Query: 148 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 207
           TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD
Sbjct: 121 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 180

Query: 208 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 267
           KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD
Sbjct: 181 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 240

Query: 268 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAKSYQL 327
           EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEIL+QLGADALKKAIEDAKSYQL
Sbjct: 241 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILVQLGADALKKAIEDAKSYQL 300

Query: 328 CDTHQIQVEEDLESFHEPS 346
           CDT +IQVEEDLESFHEPS
Sbjct: 301 CDTDEIQVEEDLESFHEPS 319

BLAST of Cp4.1LG09g07090 vs. ExPASy TrEMBL
Match: A0A6J1K3X3 (primase homolog protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490430 PE=4 SV=1)

HSP 1 Score: 649 bits (1674), Expect = 1.76e-233
Identity = 316/323 (97.83%), Postives = 317/323 (98.14%), Query Frame = 0

Query: 24  VKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 83
           VKVLMEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS
Sbjct: 88  VKVLMEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQS 147

Query: 84  ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 143
           ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN
Sbjct: 148 ECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRN 207

Query: 144 VVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 203
           VVMQTA RQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE
Sbjct: 208 VVMQTAGRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVE 267

Query: 204 GEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILA 263
           GEMDKLSVEEAGFLNCISVPGGAP KVSTNAVASIQKDTAYQYLWSCKDLLDKVPR+ILA
Sbjct: 268 GEMDKLSVEEAGFLNCISVPGGAPNKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRVILA 327

Query: 264 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 323
           TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK
Sbjct: 328 TDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAK 387

Query: 324 SYQLCDTHQIQVEEDLESFHEPS 346
           SYQLCDT  IQVE DLESFHEPS
Sbjct: 388 SYQLCDTDHIQVEGDLESFHEPS 410

BLAST of Cp4.1LG09g07090 vs. ExPASy TrEMBL
Match: A0A6J1K2E1 (primase homolog protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490430 PE=4 SV=1)

HSP 1 Score: 643 bits (1659), Expect = 1.15e-232
Identity = 312/319 (97.81%), Postives = 313/319 (98.12%), Query Frame = 0

Query: 28  MEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 87
           MEKMDLIGIVCDE CTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW
Sbjct: 1   MEKMDLIGIVCDEQCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCFQSECGW 60

Query: 88  AGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 147
           AGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ
Sbjct: 61  AGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLKRNVVMQ 120

Query: 148 TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 207
           TA RQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD
Sbjct: 121 TAGRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELIIVEGEMD 180

Query: 208 KLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRIILATDSD 267
           KLSVEEAGFLNCISVPGGAP KVSTNAVASIQKDTAYQYLWSCKDLLDKVPR+ILATDSD
Sbjct: 181 KLSVEEAGFLNCISVPGGAPNKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRVILATDSD 240

Query: 268 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAKSYQL 327
           EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAKSYQL
Sbjct: 241 EPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIEDAKSYQL 300

Query: 328 CDTHQIQVEEDLESFHEPS 346
           CDT  IQVE DLESFHEPS
Sbjct: 301 CDTDHIQVEGDLESFHEPS 319

BLAST of Cp4.1LG09g07090 vs. ExPASy TrEMBL
Match: A0A6J1K0C5 (primase homolog protein isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111490430 PE=4 SV=1)

HSP 1 Score: 570 bits (1470), Expect = 2.15e-204
Identity = 280/286 (97.90%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 61  MERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILE 120
           MERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILE
Sbjct: 1   MERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILE 60

Query: 121 PLGDELITYFSKRMISQETLKRNVVMQTADRQAAIAFTYRQNGQLVGCKYRTINKKFWQA 180
           PLGDELITYFSKRMISQETLKRNVVMQTA RQAAIAFTYRQNGQLVGCKYRTINKKFWQA
Sbjct: 61  PLGDELITYFSKRMISQETLKRNVVMQTAGRQAAIAFTYRQNGQLVGCKYRTINKKFWQA 120

Query: 181 KGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQK 240
           KGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAP KVSTNAVASIQK
Sbjct: 121 KGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPNKVSTNAVASIQK 180

Query: 241 DTAYQYLWSCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRF 300
           DTAYQYLWSCKDLLDKVPR+ILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRF
Sbjct: 181 DTAYQYLWSCKDLLDKVPRVILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRF 240

Query: 301 KDANEILMQLGADALKKAIEDAKSYQLCDTHQIQVEEDLESFHEPS 346
           KDANEILMQLGADALKKAIEDAKSYQLCDT  IQVE DLESFHEPS
Sbjct: 241 KDANEILMQLGADALKKAIEDAKSYQLCDTDHIQVEGDLESFHEPS 286

BLAST of Cp4.1LG09g07090 vs. TAIR 10
Match: AT1G30680.1 (toprim domain-containing protein )

HSP 1 Score: 310.1 bits (793), Expect = 2.3e-84
Identity = 157/330 (47.58%), Postives = 214/330 (64.85%), Query Frame = 0

Query: 5   SYSAVAIATTRVNVMGESKVKVLMEKMDLIGI------VCDEHCTPGKYYCLLCPKCKGG 64
           SY+++    T V+   E+  +V++ ++  +        V  E+C PG++  L+CP C+GG
Sbjct: 83  SYNSIPRVPTPVDTEVEADKRVVLSRLVTLRRKLAEQGVDAENCPPGQHSGLICPTCEGG 142

Query: 65  RLMERSLSLHVIPTGDFAMWRCFQSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLI 124
              E+SLSL + P G  A W CF+ +CG  G + ADG LA        K+      + + 
Sbjct: 143 NSGEKSLSLFIAPDGSSATWNCFRGKCGLKGGVRADGGLA--SADPIEKVERKITVEGIE 202

Query: 125 LEPLGDELITYFSKRMISQETLKRNVVMQ-TADRQAAIAFTYRQNGQLVGCKYRTINKKF 184
           LEPL DE+  YF+ R IS++TL+RN VMQ     +  IAFTY Q G+LV CKYR++ K F
Sbjct: 203 LEPLCDEIQDYFAARAISRKTLERNRVMQKRIGDEIVIAFTYWQRGELVSCKYRSLTKMF 262

Query: 185 WQAKGTEKLFYGIDDINDADELIIVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVAS 244
           +Q + T ++ YG+DDI    E+IIVEGE+DKL++EEAGFLNC+SVP GAP KVS+  + S
Sbjct: 263 FQERKTRRILYGLDDIEKTSEVIIVEGEIDKLAMEEAGFLNCVSVPDGAPAKVSSKEIPS 322

Query: 245 IQKDTAYQYLWSCKDLLDKVPRIILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDF 304
             KDT Y++LW+C D L K  RI++ATD D PGQA+AEE+A RLGK RCWRV WP K + 
Sbjct: 323 EDKDTKYKFLWNCNDYLKKASRIVIATDGDGPGQAMAEEIARRLGKERCWRVKWPKKSED 382

Query: 305 NRFKDANEILMQLGADALKKAIEDAKSYQL 328
             FKDANE+LM  G   LK+AI DA+ Y +
Sbjct: 383 EHFKDANEVLMSKGPHLLKEAILDAEPYPI 410

BLAST of Cp4.1LG09g07090 vs. TAIR 10
Match: AT1G30660.1 (nucleic acid binding;nucleic acid binding )

HSP 1 Score: 303.5 bits (776), Expect = 2.1e-82
Identity = 155/307 (50.49%), Postives = 203/307 (66.12%), Query Frame = 0

Query: 22  SKVKVLMEKMDLIGIVCDEHCTPGKYYCLLCPKCKGGRLMERSLSLHVIPTGDFAMWRCF 81
           SK+  LM K+   GI   ++C PG   CL+CPKC+ G   E+SL+L++ P G  A W C 
Sbjct: 33  SKLVTLMRKLSEQGIDA-QNCPPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC- 92

Query: 82  QSECGWAGRIFADGRLAFNEFSRTAKLFGGTVKKSLILEPLGDELITYFSKRMISQETLK 141
           + +CG  G +  DG+L   +     K+      +S+ LEPL DE+  +F+ R IS +TL+
Sbjct: 93  RRKCGLKGVLQVDGKLVSKD--PIGKVERKITVESIKLEPLCDEIQDFFAARAISGKTLE 152

Query: 142 RNVVMQ-TADRQAAIAFTYRQNGQLVGCKYRTINKKFWQAKGTEKLFYGIDDINDADELI 201
           RN VMQ   D +  IAFTY Q G+LV CKYR++ KKF Q + T K+ YG+DDI +  E+I
Sbjct: 153 RNRVMQKRIDDEIVIAFTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEII 212

Query: 202 IVEGEMDKLSVEEAGFLNCISVPGGAPTKVSTNAVASIQKDTAYQYLWSCKDLLDKVPRI 261
           IVEGE DKL++EEAGF NC+SVP GAP  VS+  + S  KDTA++Y+W+C D L K  RI
Sbjct: 213 IVEGEPDKLAMEEAGFFNCVSVPDGAPETVSSKEIPSESKDTAFKYIWNCNDYLKKASRI 272

Query: 262 ILATDSDEPGQALAEELALRLGKHRCWRVDWPYKDDFNRFKDANEILMQLGADALKKAIE 321
           ++ATD D PGQALAEELA RLGK RCW V WP K +   FKDANE+LM  G   LK+AI 
Sbjct: 273 VIATDGDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKDANEVLMSKGPHLLKEAIL 332

Query: 322 DAKSYQL 328
           +A+ Y L
Sbjct: 333 NAEPYPL 335

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B5X5823.2e-8347.58Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
F4I6E63.0e-8150.49Primase homolog protein OS=Arabidopsis thaliana OX=3702 GN=At1g30660 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023541322.19.33e-240100.00primase homolog protein-like [Cucurbita pepo subsp. pepo][more]
XP_022955059.11.48e-23598.45primase homolog protein-like isoform X1 [Cucurbita moschata][more]
KAG6573428.14.58e-23598.14Primase-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022955060.11.75e-23498.43primase homolog protein-like isoform X2 [Cucurbita moschata][more]
XP_022994814.13.64e-23397.83primase homolog protein isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GUU97.18e-23698.45primase homolog protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1GU468.47e-23598.43primase homolog protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K3X31.76e-23397.83primase homolog protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490430 P... [more]
A0A6J1K2E11.15e-23297.81primase homolog protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490430 P... [more]
A0A6J1K0C52.15e-20497.90primase homolog protein isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111490430 P... [more]
Match NameE-valueIdentityDescription
AT1G30680.12.3e-8447.58toprim domain-containing protein [more]
AT1G30660.12.1e-8250.49nucleic acid binding;nucleic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006171TOPRIM domainSMARTSM00493toprim5coord: 197..286
e-value: 2.1E-6
score: 37.3
IPR006171TOPRIM domainPFAMPF13662Toprim_4coord: 197..289
e-value: 5.1E-18
score: 65.3
IPR006171TOPRIM domainPROSITEPS50880TOPRIMcoord: 197..315
score: 9.651064
NoneNo IPR availableGENE3D3.40.1360.10coord: 184..326
e-value: 2.9E-32
score: 113.3
NoneNo IPR availablePANTHERPTHR12873:SF1ACID-BINDING PROTEIN, PUTATIVE-RELATEDcoord: 10..326
NoneNo IPR availableSUPERFAMILY56731DNA primase corecoord: 129..309
IPR027032Twinkle-like proteinPANTHERPTHR12873T7-LIKE MITOCHONDRIAL DNA HELICASEcoord: 10..326
IPR034154Archaeal primase DnaG/twinkle, TOPRIM domainCDDcd01029TOPRIM_primasescoord: 197..289
e-value: 2.29872E-11
score: 57.2802

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g07090.1Cp4.1LG09g07090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032508 DNA duplex unwinding
molecular_function GO:0043139 5'-3' DNA helicase activity
molecular_function GO:0003697 single-stranded DNA binding