Cp4.1LG09g06170 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g06170
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionATP-dependent DNA helicase Q-like 5
LocationCp4.1LG09: 3951925 .. 3960284 (+)
RNA-Seq ExpressionCp4.1LG09g06170
SyntenyCp4.1LG09g06170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGGGCAAAATAGGAAACCTTCGACACATCTTCATCCGTTGCTCTCCCTCCAATTTTCCGTCGCCATTACCCGACATGAATTCCGATTCAGACTCAGATTCAGATGCTTCTCACATCTCCGCCACTCCTCCCAGAGATTCAAATCCGCCGCCGCCGCCGCTGCCGTCGTCTTCTTCAGTTACTCCGTTCACGTCAGCTTCCAATGCTAAGTGTAAGTTCAGTAAAGCCACTTCTTCTCTGGCGCGTACCAGGTCGAAACCTGGAAAGCACTCTTCCAGAGTTGCTAAGCCGTCTCCACCGCCTGATACGAAACCCTCCGAGGAAGAATCGCTTCCCGACTGGACTCCCCTTCCAACTCTGCCATATCAAATCCGTCGGGCTTCGGATCAGAGTCGTGGAGTTTCGTCCTCTGAATCTATCGAGATGCTTCCAGCCGGGTTCTTTTCAAAATCGTCATCGTTCTTGAAATTCCGTGGATCCTGTCTTAATTTTGAGACTTCCGAGGAAAATCGGTCTTTATCGGAGCCGAGCCAGCAGAACAATGCTGAGACTGAGGTCGCTAGTTCTTCAACGGCTGATTGGGGATCAAAAGATAATTTGTTTAGTCCCGGGAATCCGGTGAAACTCGTTAGGAAATATCCTAACTTGATTGGAGCCCACGTTCCTGCTCCGCCAGTCAAAGTGCGCAAATGTGGAGGTGAAGGCAACTTTGTGAAGCTTAACTTGAATGGTAGAAAGCGAAAATTTATTAAGAAATTCAACCGGAGAAAATATGGCGAGCGGAGTAGTTACCGGTCCCATAGAAGGACTAAGATGAACTCGAGAAATGAAGATTGCAACGAGACGGCTAGTTTCTGCGATGAAGATGGTTTGGTTACAGAAACAACGCAACATCCACAGAAGCAGGGCAATGGAGGAGCGAAATTTGATCCCGTTTCCATTGAAGAGACTATATCCAATGTGCGAAATGACCCATCTGATGATAACTTGGTAAAGTTGTTGAAATTAGCTTATGGGTATGATTCTTTCCAAGAAGGGCAGTTAGAGACTATCAAAATGGTGCTGGCCGGGAAATCAACGATGGTTGTTTTGCCCACCGGTGCCGGTAAATCAATTTGTTATCAAATACCGGCAATGGTTCTACCTGGGATAACCGTTGTTGTTAGTCCTTTGGTGGCATTAATGATAGATCAGATAAAACAATTGCCGCCTGTAATTCAGGGTGGTTTATTATGCAGCAGTCAGGTAGTCGCTGCTCTATTTCTCTATCAAGCTTTTTAAACGTTTTTGCTTTTGTATGATGACAGTGTGAAAGAGTGTGAAACTTTACTTGATCGTTCTTGTGTATTTGTAGAGGCCTGATGAAGTATCTGAAATAGTGAGGCTGCTTATCCAAGGGTCGATGAAGGTACATGAATGGGAAAATTCCCATACTTTTCTCTGTCTATTGCAAAACATTATCGTGAGAACAACTGCATTGATTCGTTATCTTGCATTTAAATGTTACAAGTATTATTCGGATGAGTGTTAGAAATCACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGACTTTGCTTTGGGCTTTCCCAAAAGGCCTCGTACCAATGGAGATGTATTCCTTACTTATAAACCCATGATCATTCTCTAAATTAGCCAACGTGGGACTCCCTCCCAAGAATCCTTTATAGTTGGAATGTCATCAGTCATGGCGTGGTTAAGTTCTTATATGTATTTTCAGGTGCTCTTTGTTTCACCTGAAAGGTTTCAGAACCCAGATTTTCTGTCAATGTTTTCTAGCGGCTTAGTGGTATCTCTTCTTGTGGTGGATGAAGCTCACTGTATATCTGAATGGCAAGTTGATTCCCAATTTGAACCGTGATCGGCATCATTTTCTTATTTAGACACTCCACTTATGAGACCTCTGGCTTTCAGCATTTGCTATGGCATGAATGAACTAGTCTAAGTTATTCAATTTGATATGACCAGGTCACACAATTTTCGACCTTCATATATGAGGCTTAGAGCATCTCTACTTCGAGCTCAACTCAACATTAACTGCATTCTTGCAATGACCGCAACGGCAACTACTTCAACTATGCAAGCAATTATGACTGCGTTAGAAATTCCTTCAGATAATCTCATTCTGAAGACTACAGTAAGGACAAATCTACAATTGTCAGTATCAATGAGTACAAACAGGCAAGTATTAAATCTTGAGAGTTGATTTTTCTCCTTCTTAACCACTTTAATTTTATCATATGGATCCTTTTAATTAACACTTTCAATGTTTCAATTTGATTTGGTGATGTGCTGAAAATAAGAGTGAAAGATCTACTTAAGCTAATCAAGTCTTCTCCCATAGCTGAAGTTCGGAGCATTATTATCTACTGCAAGTTTCAGGTTTGTTTCTGTCAGTAATTTATTATCATAATACATAGAAGTTTGAATGAAGCTTAAGCTCACCCTCTAGGTCTGTAGCTGTCTTTTGAGTTAGTGTGGAATGAAACGCTCAAATTAACATTATTATGTCTTTTCCCATTTATTTCATTTTCTGAATGGGCCAATTTTGTCATTAACTTTGAACTGTTTCGTTCCTACGTAATTAAGTATTTTTGCAGAGGAAAGTTTGGTTGAACCCTTAGCTGATCTGTAGTTGTTTGTTCATCATTTTGCACAGTGACCTGATAGTGTTTCTCAATTTTCATTAATCGTCTAGATAAATTTTGTAACTTTTAGTTAGCCATGACTGCTTCTCTGCTCTGATGCTGCAGTTGGAAACTGACTCGGTAAGCAGATACTTGTGTGATAATGGCATCTCTGCAAAGGTTACTTCTGCATCTTAGCTAGCTTATGAATAACAACTACTATTACCTTTTTGGGGTATTTGAAAAGTAAATCTGATCGGTTGCTTCCTATTTTGCTAATTAGAGTTATCACAGTGGTCTTCCTGCTAAAGATCGCAAACACATACAGGACAATTTTTGTTCAAACAAGATTAGAGTGGTGAGATACCATATTCTTCTATTATTTGTGTGAATGTATGTATATAAATGTTATGAATAATTATTTCCTATCTCAATGCATTGACTGACACTTTTTCTAAAGGTTGTTGCTACTGTGGCTTTTGGCATGGGACTTAACAAGAGGGACGTTGGCGCTGTAAGAATCTAGAGAGCATTTTCAAATTGTTTTATTCCTTATTTGTGTGAGGATATTCCTGATGTATTGAAATGGTTGACTAGCAATGTAAGAGTAGTATGAACATCTTTCTGTATCGGATCCGCTCATCTCTCTAAAGATGCTCCATGTCAGGAATGTTAGATTTTTTCTTTTTTTTTTTTTCAAAGCATGTGCTTGATTTTTTATTTGTTTCAAAAAGGAGTGCGAGATCTAATTTTGAAGTCAGTTGAATACATCAATGCCACATCCTTGTGCATGTAATGTAATAAATAGTTAGCTGTGGAGGATTACAATAACGTGAAATCACTTAGGTGGACCACATTGTACATGAGATAAATGTTCGAGGCACAAATTTAGCTTTTATTGGTTTTGGAATATTTGTACTATATGAACGATGATATCTAAACAGTAAAAAAGTAAAGTTAAATTGTTTCAGGTAATCCATTATAGCATGCCGGAAAGCTTGGAGGAATATATTCAAGTCGGTAGCTTCTTCTAATATTGTTCATTTTCTAATATGACATGTATGTTATGAACATTCTATATAGATTAATAGTACACTTATTGCAGGAGATAGGGCGTGCAGGCCGTGATGGTAGATTATCCTACTGTCATCTATTTCTTGACAATGACACATATTTCAAGCTTCGAAGTCTTATGCACAGGTCATGTGATATAAAATTTCTCGTTTTGTTAGCTATTCAAGGATTATATATTCTGGAACTGATTTGAAGTATTGTATGAACAGTGATGGAGTTGATGAATACAATATAAACAAATTCATCAGTGAAGTGTTCTCTGAAAATAAGTGTGGAAAAGTGTATTCAATGGTCAAAGATCCTGCATCTCGTAAATTTGATATGAAGGAAGAGGTCACTTTCTTCCACTGTTTTTGCAACTATCTAATTTTTTTACTTTGGAGCATTGAGGTGACAAACTTAACTGTTATTATCGCCTATGGATATAGTCACAATTTCAAATGAATATACATTGGCTGTGGTGTTTGCAAGAAGTATTAGGAAAATCTACCATCATGGAAATCTTTTGTTAGTTAGAAACTCCATTAATCTGTTAGGAAGAATTGATGTAACAGCACTACTTACAATTTGGAGAAACATGGCTCAATTAAGAGAAAAAAATCAAGAAAGGGAGTAGAACCACGCCAATTCTTTTGCGGGAAGGATTCTAAATCTCCCATCAACTTAAGGAACCAAAGCAAATTGTAGGATGCTGAAAAGCAACTAAATTCTAGAAGTAGATTCTTATGCGTAATTCAAGTCAGAGTTTTAGTTGGTGAGCAAAACATATAAACTTTTTGAAAAGTCAGAAGCCATCTCAACTTAAATCACTCTACTGTTCTCCATTACCGTGTATTAAGTCCTTGTATGCAACTTTTTCATCCCTACATATTCTTCCACATGTTCATTGCTTTAGAAATTTATCAAGTAATATAATGATGATGTAAATATAGAAGCCATGGAGATCTTTTAACTGCTGGAATTTTTTTTAGTGAAAATGGAACCTCCTTACCGAACTTATCCATTATTTTTTTTAGGAGAAGTTGTGTTCTCACTTGGTTTGATCTCCGACAGGTGATGATTACAATTCTAACATACTTGGAGCTCGGAGAAATGCAATATTTGCGCTTACTTCCTCAGTTAAATGTGACTTGCACTTTGAATTTTCATAAGGTTGATCTTCAATGATTCTTTTCTCTCTTACTTTCTGACTATGAAAGCTTAATTAAAATTTGATTGCTTCATGACTATGGCTATTATAGTTCCTACAGCCTTTTACTAAATTAATGGTATCATTCCTGATAGATGTTCAGTATCTCAGCATATATTGATCCATCTACTTGTGTCATGCATTTGAAAAATGTAAATATTCATGATATGTTGCAGTCAATATTTGGTGAAAGGGTTAGGATAGGTTCGTTTCTTGGTGCTTGATGATTTTGCGTATTCTGTGTTCGGTGCAGACTTCACCAGCGATGCTTGCTGACAAAGATATCATTGTTGCTGAAATTTTGAAGAAGTGAGTACCTAGTTTTGATCTCGACCAATCTAATACTATGCACTATTGGGAGAAAAAAAATTGCTTTTCGTATCAGGCTATTAAACTTGGGAACTTTAGGTCTGAAACTAAGCAAGGGCAGCATGTTTTTGACATGCTCACGGTGGTCAATAGCATTGGGATTTCAGCTACCAGTCTCTCAAATCATTTGCAAAATCTCAAGGTTCGGATGATTTTAAATGGAAAACTCAACATCTCCTTTATTTTCTTTTCATTCCATATTTACAACAGTTAGTTATTGTTTAAGCTTTATTCCAATATAACCTTGCATTTTAAATTTAGTTACTCTCAACTACTCAATGTATCAGTGTTTATCAACTTTGATTGATATATAAATATTCAATTTGAAGAATTGAAGATGAATGAATGCATGTTAAAAATAGGCCCTTTTTCAATTTTATCTATGCAGTTGCATGCATGAATATAATTTAGAAAGACGTTAGACTAATTGTTCGTCGATTTTAAACGGATGTAGCATGCAACTTTGGTTTCGATAATCCTAGAGAAGATTTTTGGCGATACGTCGAGCGATCTAAAAAGTTATTGAGAAGTTATATCATGTGTTATATTCTTTTGCTTTTGTAATTTTGTCTCTATTATCTTCTATTTCTTTTATGTAACAAAGTTTCTTTCTTATACTCTTTTAGTTGAAGGGAGAAATCACATACGAAATGAAGGATCTGGCGTACTGTTATACAATTTTGAAATATCCTGAAGATTTTTGTTCTCTGTCAGCGCATCTTAGAAAATGGTTAGCCGAGATTCAGACTAGTAAGGTTGTTGATTCTGACTTTACTATGCATTTGTGAATCTATTTATAATACCATAACAGATGGCCGTGATACTACCACAAAACTTGTAATTGTGGAGCCTTTTTTTGATCTATACTTCTCATCCACTAAAGTTTTGATTCACTTGCACCTACTTTTCATTCAAGAAAACTTGAAAGAATTGGTTCATTACGTCTGCTGATTTGTATTTATTCACCCAAAGCACCAAAATTTTTGTTAAGATATATTGTTGTTACCTTCCATTTTCAACACCTTTGGGCCAAAGCTCTGTTGTTCTGACTGGGTAATTTACGTGCAAGCTAGCTCATCTACTCGTGAGTGTCCCCTCGTAGATCAACATTCGGGAGGATGTTGTATGTTTTTCTTTAAATCAAACCGACATGTCTAGGGGTTGTTTTTGTTTTCAGGTTAGGAAACTGGACGCCATGTTTGATGCGGTGACTTCGGCTGTGAATTTGTATGAAAAGAAGACTCAGGGCTGCTTTAACTATGAGCAAACACCATGCTTAGAAGGAAAGATTTGTTCTTATTTCCAGGAGGGTGATAATTATGATATTCCAAATAAAATGAACCCGAGCAGGTATGTACAGTCACTATTTCTGATATACAGTTAGAAGAAATGAGAAGAGATTTGACCAGTAGTGTAACGACCCAAGCCCACCGCTAGTATATATTATCCTCTTTGGGTTTTTCCTTTCGAGCTTCTCCTTAAAAATTTTAAAACGCATATGTTAGATAACCGATGGGGGATCTCATAGTCCACCTTCCTTCAGAGCCCAGCGTCCGCGCGGACACTCGTTCCCTTCTCCAATCGATGTGAGACCCCTCAATCCACAACATTCTTGCTGGCACACCGTCCCATGTAAGGGCCCAACGTCTTTGCTGGCACACCATCCCGTGCCACCCCCCTTTGAAGTTTAGCCTCCTTGCTGGCACATCGCTCGGTGTCTGGCTCTGATATCATTTGTAACGATCTAAGCCCACCGCTAGCAGATATTATTCTCTTTGGGCTTCCCCTCAAGGTTTTTAAATCGCATTTGCTAGGAAATGAGGTTTTTACACCCTTATAAAAAACGTTTCGTTCTTGTTTCCAACTGATGTGTGATCTCATAAGTAGATTATTCATGCAATACTATTAATTCTTGTTCTTGTTCATTTACTACGTACATGAGGTACAGCTTGCTTCTAACTTCAGTCTGGTTATAATCGTACTAACATCAATATTATTCATGTGCAGCCCATTCCTCCGAGCAGATATAAAAGTGAGGGCTATACAAATGCTTAATGAGATCATTTTCAACGGGTCAAGGCTCAGCTATGTTGAAAAAGCTTATACTTTTTGCTGTAACAGGTCTTTTTGCAGAGCAACTCACAGGCTAAGTTCACTCCCAGGGCCGTTGCAAGGATTATGCATGGCATTTCGAGCCCCGCTTATCCTTCAACAATATGGTCAAGAACTCATTTCTGGTAACCAGATTTCCTTTTAGTTTCAATCAAATTCTCGTACATCACCATTAGCAAATACTTAGCTGACGAACCACCTTTAGATCACGCTCACCATGCCAAATATGAATTTGTCTAAACTTGTAGGAAGAAAGAAAGTCTGTCACCTTAGGAAAACATTGAAAATTCATAATTTGCCTGAAATATAAGCAACCAATCTGATTGTAAGCACATTTGGGATGACAGGGGAAGGTATACACAGGTCGATTTCTCGGTAATAATGGAAGCAGCTACGTTAGAGCTCTTAAATTTTGTCGGGAAGGACGTCGTGTCCTAGCTATACATGAACAGATAGATCTTTTTCAAAACTGTTGTTGAGCATCTAGATCCACAGGCATAGCAGTTGGGTGATTCAAAACATGGTCATCTCATTCTCATCAATCTACCATTGCCATGATTACCACTCAAGTAGTTAAGAAGCAGATGGTCCATTTTGTTGTCCGCCGAGCCTAATGATCAAGAGGAGGACAAACAAGATCGATGTCAAGAAAGGTAGATTAACTGAATCACTGACAGACTTTCATAGATTTTTCTTAACTTTCTGTCAGTTGTGAATTAATTGGTGTTTGCAGGAGAACATATTGGAATCACAAGGGATGGAGTTCATCAAGTTTTCTGTTGGTTTTTGTACAGATTTATTGATTACTAAGCCCCATTTGCCTTGTTCATCTGTACTTCCTGCAACTAGTGTAACTTATACCACAAGTAAAGTAAAAAAAAAAAAAAAAAATTAAAAAACTAGGAATAATTATGCCTTTTTTCATTATTCTTTTTATTATTTTATAATCCT

mRNA sequence

TCGGGCAAAATAGGAAACCTTCGACACATCTTCATCCGTTGCTCTCCCTCCAATTTTCCGTCGCCATTACCCGACATGAATTCCGATTCAGACTCAGATTCAGATGCTTCTCACATCTCCGCCACTCCTCCCAGAGATTCAAATCCGCCGCCGCCGCCGCTGCCGTCGTCTTCTTCAGTTACTCCGTTCACGTCAGCTTCCAATGCTAAGTGTAAGTTCAGTAAAGCCACTTCTTCTCTGGCGCGTACCAGGTCGAAACCTGGAAAGCACTCTTCCAGAGTTGCTAAGCCGTCTCCACCGCCTGATACGAAACCCTCCGAGGAAGAATCGCTTCCCGACTGGACTCCCCTTCCAACTCTGCCATATCAAATCCGTCGGGCTTCGGATCAGAGTCGTGGAGTTTCGTCCTCTGAATCTATCGAGATGCTTCCAGCCGGGTTCTTTTCAAAATCGTCATCGTTCTTGAAATTCCGTGGATCCTGTCTTAATTTTGAGACTTCCGAGGAAAATCGGTCTTTATCGGAGCCGAGCCAGCAGAACAATGCTGAGACTGAGGTCGCTAGTTCTTCAACGGCTGATTGGGGATCAAAAGATAATTTGTTTAGTCCCGGGAATCCGGTGAAACTCGTTAGGAAATATCCTAACTTGATTGGAGCCCACGTTCCTGCTCCGCCAGTCAAAGTGCGCAAATGTGGAGGTGAAGGCAACTTTGTGAAGCTTAACTTGAATGGTAGAAAGCGAAAATTTATTAAGAAATTCAACCGGAGAAAATATGGCGAGCGGAGTAGTTACCGGTCCCATAGAAGGACTAAGATGAACTCGAGAAATGAAGATTGCAACGAGACGGCTAGTTTCTGCGATGAAGATGGTTTGGTTACAGAAACAACGCAACATCCACAGAAGCAGGGCAATGGAGGAGCGAAATTTGATCCCGTTTCCATTGAAGAGACTATATCCAATGTGCGAAATGACCCATCTGATGATAACTTGGTAAAGTTGTTGAAATTAGCTTATGGGTATGATTCTTTCCAAGAAGGGCAGTTAGAGACTATCAAAATGGTGCTGGCCGGGAAATCAACGATGGTTGTTTTGCCCACCGGTGCCGGTAAATCAATTTGTTATCAAATACCGGCAATGGTTCTACCTGGGATAACCGTTGTTGTTAGTCCTTTGGTGGCATTAATGATAGATCAGATAAAACAATTGCCGCCTGTAATTCAGGGTGGTTTATTATGCAGCAGTCAGAGGCCTGATGAAGTATCTGAAATAGTGAGGCTGCTTATCCAAGGGTCGATGAAGGTGCTCTTTGTTTCACCTGAAAGGTTTCAGAACCCAGATTTTCTGTCAATGTTTTCTAGCGGCTTAGTGGTATCTCTTCTTGTGGTGGATGAAGCTCACTGTATATCTGAATGGCAAGTTGATTCCCAATTTGAACCGTCACACAATTTTCGACCTTCATATATGAGGCTTAGAGCATCTCTACTTCGAGCTCAACTCAACATTAACTGCATTCTTGCAATGACCGCAACGGCAACTACTTCAACTATGCAAGCAATTATGACTGCGTTAGAAATTCCTTCAGATAATCTCATTCTGAAGACTACAGTAAGGACAAATCTACAATTAGTGAAAGATCTACTTAAGCTAATCAAGTCTTCTCCCATAGCTGAAGTTCGGAGCATTATTATCTACTGCAAGTTTCAGTTGGAAACTGACTCGGTAAGCAGATACTTGTGTGATAATGGCATCTCTGCAAAGAGTTATCACAGTGGTCTTCCTGCTAAAGATCGCAAACACATACAGGACAATTTTTGTTCAAACAAGATTAGAGTGGTTGTTGCTACTGTGGCTTTTGGCATGGGACTTAACAAGAGGGACGTTGGCGCTGTAATCCATTATAGCATGCCGGAAAGCTTGGAGGAATATATTCAAGAGATAGGGCGTGCAGGCCGTGATGGTAGATTATCCTACTGTCATCTATTTCTTGACAATGACACATATTTCAAGCTTCGAAGTCTTATGCACAGTGATGGAGTTGATGAATACAATATAAACAAATTCATCAGTGAAGTGTTCTCTGAAAATAAGTGTGGAAAAGTGTATTCAATGGTCAAAGATCCTGCATCTCGTAAATTTGATATGAAGGAAGAGGTGATGATTACAATTCTAACATACTTGGAGCTCGGAGAAATGCAATATTTGCGCTTACTTCCTCAGTTAAATGTGACTTGCACTTTGAATTTTCATAAGACTTCACCAGCGATGCTTGCTGACAAAGATATCATTGTTGCTGAAATTTTGAAGAAGTCTGAAACTAAGCAAGGGCAGCATGTTTTTGACATGCTCACGGTGGTCAATAGCATTGGGATTTCAGCTACCAGTCTCTCAAATCATTTGCAAAATCTCAAGTTGAAGGGAGAAATCACATACGAAATGAAGGATCTGGCGTACTGTTATACAATTTTGAAATATCCTGAAGATTTTTGTTCTCTGTCAGCGCATCTTAGAAAATGGTTAGCCGAGATTCAGACTAGTAAGGTCTTTTTGCAGAGCAACTCACAGGCTAAGTTCACTCCCAGGGCCGTTGCAAGGATTATGCATGGCATTTCGAGCCCCGCTTATCCTTCAACAATATGGTCAAGAACTCATTTCTGGGGAAGGTATACACAGGTCGATTTCTCGGTAATAATGGAAGCAGCTACGTTAGAGCTCTTAAATTTTGTCGGGAAGGACGTCGTGTCCTAGCTATACATGAACAGATAGATCTTTTTCAAAACTGTTGTTGAGCATCTAGATCCACAGGCATAGCAGTTGGGTGATTCAAAACATGGTCATCTCATTCTCATCAATCTACCATTGCCATGATTACCACTCAAGTAGTTAAGAAGCAGATGGTCCATTTTGTTGTCCGCCGAGCCTAATGATCAAGAGGAGGACAAACAAGATCGATGTCAAGAAAGGAGAACATATTGGAATCACAAGGGATGGAGTTCATCAAGTTTTCTGTTGGTTTTTGTACAGATTTATTGATTACTAAGCCCCATTTGCCTTGTTCATCTGTACTTCCTGCAACTAGTGTAACTTATACCACAAGTAAAGTAAAAAAAAAAAAAAAAAATTAAAAAACTAGGAATAATTATGCCTTTTTTCATTATTCTTTTTATTATTTTATAATCCT

Coding sequence (CDS)

ATGAATTCCGATTCAGACTCAGATTCAGATGCTTCTCACATCTCCGCCACTCCTCCCAGAGATTCAAATCCGCCGCCGCCGCCGCTGCCGTCGTCTTCTTCAGTTACTCCGTTCACGTCAGCTTCCAATGCTAAGTGTAAGTTCAGTAAAGCCACTTCTTCTCTGGCGCGTACCAGGTCGAAACCTGGAAAGCACTCTTCCAGAGTTGCTAAGCCGTCTCCACCGCCTGATACGAAACCCTCCGAGGAAGAATCGCTTCCCGACTGGACTCCCCTTCCAACTCTGCCATATCAAATCCGTCGGGCTTCGGATCAGAGTCGTGGAGTTTCGTCCTCTGAATCTATCGAGATGCTTCCAGCCGGGTTCTTTTCAAAATCGTCATCGTTCTTGAAATTCCGTGGATCCTGTCTTAATTTTGAGACTTCCGAGGAAAATCGGTCTTTATCGGAGCCGAGCCAGCAGAACAATGCTGAGACTGAGGTCGCTAGTTCTTCAACGGCTGATTGGGGATCAAAAGATAATTTGTTTAGTCCCGGGAATCCGGTGAAACTCGTTAGGAAATATCCTAACTTGATTGGAGCCCACGTTCCTGCTCCGCCAGTCAAAGTGCGCAAATGTGGAGGTGAAGGCAACTTTGTGAAGCTTAACTTGAATGGTAGAAAGCGAAAATTTATTAAGAAATTCAACCGGAGAAAATATGGCGAGCGGAGTAGTTACCGGTCCCATAGAAGGACTAAGATGAACTCGAGAAATGAAGATTGCAACGAGACGGCTAGTTTCTGCGATGAAGATGGTTTGGTTACAGAAACAACGCAACATCCACAGAAGCAGGGCAATGGAGGAGCGAAATTTGATCCCGTTTCCATTGAAGAGACTATATCCAATGTGCGAAATGACCCATCTGATGATAACTTGGTAAAGTTGTTGAAATTAGCTTATGGGTATGATTCTTTCCAAGAAGGGCAGTTAGAGACTATCAAAATGGTGCTGGCCGGGAAATCAACGATGGTTGTTTTGCCCACCGGTGCCGGTAAATCAATTTGTTATCAAATACCGGCAATGGTTCTACCTGGGATAACCGTTGTTGTTAGTCCTTTGGTGGCATTAATGATAGATCAGATAAAACAATTGCCGCCTGTAATTCAGGGTGGTTTATTATGCAGCAGTCAGAGGCCTGATGAAGTATCTGAAATAGTGAGGCTGCTTATCCAAGGGTCGATGAAGGTGCTCTTTGTTTCACCTGAAAGGTTTCAGAACCCAGATTTTCTGTCAATGTTTTCTAGCGGCTTAGTGGTATCTCTTCTTGTGGTGGATGAAGCTCACTGTATATCTGAATGGCAAGTTGATTCCCAATTTGAACCGTCACACAATTTTCGACCTTCATATATGAGGCTTAGAGCATCTCTACTTCGAGCTCAACTCAACATTAACTGCATTCTTGCAATGACCGCAACGGCAACTACTTCAACTATGCAAGCAATTATGACTGCGTTAGAAATTCCTTCAGATAATCTCATTCTGAAGACTACAGTAAGGACAAATCTACAATTAGTGAAAGATCTACTTAAGCTAATCAAGTCTTCTCCCATAGCTGAAGTTCGGAGCATTATTATCTACTGCAAGTTTCAGTTGGAAACTGACTCGGTAAGCAGATACTTGTGTGATAATGGCATCTCTGCAAAGAGTTATCACAGTGGTCTTCCTGCTAAAGATCGCAAACACATACAGGACAATTTTTGTTCAAACAAGATTAGAGTGGTTGTTGCTACTGTGGCTTTTGGCATGGGACTTAACAAGAGGGACGTTGGCGCTGTAATCCATTATAGCATGCCGGAAAGCTTGGAGGAATATATTCAAGAGATAGGGCGTGCAGGCCGTGATGGTAGATTATCCTACTGTCATCTATTTCTTGACAATGACACATATTTCAAGCTTCGAAGTCTTATGCACAGTGATGGAGTTGATGAATACAATATAAACAAATTCATCAGTGAAGTGTTCTCTGAAAATAAGTGTGGAAAAGTGTATTCAATGGTCAAAGATCCTGCATCTCGTAAATTTGATATGAAGGAAGAGGTGATGATTACAATTCTAACATACTTGGAGCTCGGAGAAATGCAATATTTGCGCTTACTTCCTCAGTTAAATGTGACTTGCACTTTGAATTTTCATAAGACTTCACCAGCGATGCTTGCTGACAAAGATATCATTGTTGCTGAAATTTTGAAGAAGTCTGAAACTAAGCAAGGGCAGCATGTTTTTGACATGCTCACGGTGGTCAATAGCATTGGGATTTCAGCTACCAGTCTCTCAAATCATTTGCAAAATCTCAAGTTGAAGGGAGAAATCACATACGAAATGAAGGATCTGGCGTACTGTTATACAATTTTGAAATATCCTGAAGATTTTTGTTCTCTGTCAGCGCATCTTAGAAAATGGTTAGCCGAGATTCAGACTAGTAAGGTCTTTTTGCAGAGCAACTCACAGGCTAAGTTCACTCCCAGGGCCGTTGCAAGGATTATGCATGGCATTTCGAGCCCCGCTTATCCTTCAACAATATGGTCAAGAACTCATTTCTGGGGAAGGTATACACAGGTCGATTTCTCGGTAATAATGGAAGCAGCTACGTTAGAGCTCTTAAATTTTGTCGGGAAGGACGTCGTGTCCTAG

Protein sequence

MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRSKPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPAGFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGNPVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYRSHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDPSDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGITVVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNPDFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCILAMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQLVKDLLKLIKSSPIAEVRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSDGVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKVFLQSNSQAKFTPRAVARIMHGISSPAYPSTIWSRTHFWGRYTQVDFSVIMEAATLELLNFVGKDVVS
Homology
BLAST of Cp4.1LG09g06170 vs. ExPASy Swiss-Prot
Match: Q0WVW7 (ATP-dependent DNA helicase Q-like 5 OS=Arabidopsis thaliana OX=3702 GN=RECQL5 PE=2 SV=2)

HSP 1 Score: 817.0 bits (2109), Expect = 2.1e-235
Identity = 499/965 (51.71%), Postives = 616/965 (63.83%), Query Frame = 0

Query: 4   DSDSDSDASHISATPPRDSNP-PPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRSKP 63
           D DSDSD SH+SATPPRDS P  PP L S +   P  S         K TSS +R++ K 
Sbjct: 2   DFDSDSDGSHVSATPPRDSFPSSPPQLQSPAKHVPPVS--------RKMTSSSSRSKPKA 61

Query: 64  GKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPAGF 123
             H        PPP+  PS+E  +P  +P P  P      ++    +  S+     PA F
Sbjct: 62  PTH--------PPPN--PSQEAPVP--SPYPPPPPPSPLFTNLPFRICQSQ-----PARF 121

Query: 124 FSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGNPV 183
            S  SSF +    C               S+ +    E   S   D+  +  L     P 
Sbjct: 122 SSSVSSFSRL---C---------------SRASFTSVEKLKSDGVDFVPEPPLVEVIAPP 181

Query: 184 KLV-RKYPNLIGAHVPAPPVK---VRKCG-GEGNFVKLNLNGRKRKFIKKFNRRKYGERS 243
           K V RK PNLI   + +PPVK    R  G GEGNFVKLNLNG++ K      +     RS
Sbjct: 182 KSVRRKPPNLITDTITSPPVKPMVFRSNGNGEGNFVKLNLNGKRGKKFPSKYKGVSKSRS 241

Query: 244 SYRSHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVR 303
           SY S R  +   +  D +  +   +E  L        QKQ    A     S+E+ I  V+
Sbjct: 242 SY-SFRGKRYKKKEADGDGESLLEEESDL--------QKQIEDEANGFISSVEDAILAVK 301

Query: 304 NDPSDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLP 363
            + SD+NL KLL L YGYDSF++GQL+ IKM+L G STM+VLPTGAGKS+CYQIPAM+LP
Sbjct: 302 TEASDENLTKLLNLVYGYDSFRDGQLQAIKMILGGSSTMLVLPTGAGKSLCYQIPAMILP 361

Query: 364 GITVVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERF 423
           GIT+VVSPLV+LMIDQ+K LP +I+GGLL SSQRP+E +E +R L +G +KVLFVSPER 
Sbjct: 362 GITLVVSPLVSLMIDQLKHLPSIIKGGLLSSSQRPEEATETLRKLKEGIIKVLFVSPERL 421

Query: 424 QNPDFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNIN 483
            N +FLSMF   L VSL+VVDEAHC+SEW        SHNFRPSYMRL+AS+L ++L   
Sbjct: 422 LNVEFLSMFRMSLSVSLVVVDEAHCVSEW--------SHNFRPSYMRLKASMLFSELKAE 481

Query: 484 CILAMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL---------VKDLLKLIKSS 543
           CILAMTATATT T+QA+M++LEIPS NLI K+ +R N +L         +KDLL L++S 
Sbjct: 482 CILAMTATATTMTLQAVMSSLEIPSTNLIQKSQLRDNFELSVSLSGANRMKDLLILMESP 541

Query: 544 PIAEVRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVV 603
           P  E+RSII+YCKFQ ETD +S+YL DN I+AK YHSGLPAKDR  IQ++FCSNKIRVVV
Sbjct: 542 PYKEIRSIIVYCKFQYETDMISKYLRDNNINAKGYHSGLPAKDRVRIQESFCSNKIRVVV 601

Query: 604 ATVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSL 663
           ATVAFGMGL+K DVGAVIH+S+P S+EEY+QEIGRAGRDGRLSYCHLF DNDTY KLRSL
Sbjct: 602 ATVAFGMGLDKGDVGAVIHFSVPGSMEEYVQEIGRAGRDGRLSYCHLFYDNDTYLKLRSL 661

Query: 664 MHSDGVDEYNINKFISEVFS--ENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEM 723
            HSDGVDEY + KF++ VFS    +  K+ S+V + AS+KFDMKEEVM TILT+LELGE+
Sbjct: 662 AHSDGVDEYAVGKFLTHVFSTETKQHEKICSLVIESASQKFDMKEEVMQTILTHLELGEV 721

Query: 724 QYLRLLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGIS 783
           QYLR+LPQLN+ CTLNFHK+SP  LA +  IVA ILKKS  KQG HVFD+  V +SI ++
Sbjct: 722 QYLRMLPQLNICCTLNFHKSSPNTLAARSAIVAAILKKSHVKQGLHVFDIPAVASSICVA 781

Query: 784 ATSLSNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTS------ 843
            T +   +Q LK+KGE+TYE+KD A+CYTILK P++ CSLS+HL KWL EI++       
Sbjct: 782 TTDVLAEIQALKMKGEVTYELKDSAFCYTILKSPKEICSLSSHLTKWLTEIESCKVRKLD 841

Query: 844 -----------------------------------------------------------K 887
                                                                      K
Sbjct: 842 IMSSAAVAAISVSNTSELSSGAKQTRSLQSRIFDYFNGDEKCDSPSKATQNCAFLRADIK 901

BLAST of Cp4.1LG09g06170 vs. ExPASy Swiss-Prot
Match: O94761 (ATP-dependent DNA helicase Q4 OS=Homo sapiens OX=9606 GN=RECQL4 PE=1 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 2.0e-65
Identity = 208/738 (28.18%), Postives = 328/738 (44.44%), Query Frame = 0

Query: 314  GYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVL----PGITVVVSPLVAL 373
            G+ +F+ GQ   +  +L+G ST++VLPTGAGKS+CYQ+PA++     P +T+VVSPL++L
Sbjct: 477  GHQAFRPGQERAVMRILSGISTLLVLPTGAGKSLCYQLPALLYSRRSPCLTLVVSPLLSL 536

Query: 374  MIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNPDFLSMFSSG 433
            M DQ+  LPP ++   + S     +   +++ +    + VL ++PE       L   +  
Sbjct: 537  MDDQVSGLPPCLKAACIHSGMTRKQRESVLQKIRAAQVHVLMLTPEALVGAGGLPPAAQL 596

Query: 434  LVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCILAMTATATTS 493
              V+   +DEAHC+S+W        SHNFRP Y+R+   +LR ++ ++C L +TATAT  
Sbjct: 597  PPVAFACIDEAHCLSQW--------SHNFRPCYLRV-CKVLRERMGVHCFLGLTATATRR 656

Query: 494  TMQAIMTALEIPSD-NLILKTTVRTNLQLV--------KDLLKLIKSSPIAEVRSIIIYC 553
            T   +   L +  + +L     V TNL L         + LL L++      + SIIIYC
Sbjct: 657  TASDVAQHLAVAEEPDLHGPAPVPTNLHLSVSMDRDTDQALLTLLQGKRFQNLDSIIIYC 716

Query: 554  KFQLETDSVSRYL--CDNG------------ISAKSYHSGLPAKDRKHIQDNFCSNKIRV 613
              + +T+ ++  L  C +              +A++YH+G+ +++R+ +Q  F   ++RV
Sbjct: 717  NRREDTERIAALLRTCLHAAWVPGSGGRAPKTTAEAYHAGMCSRERRRVQRAFMQGQLRV 776

Query: 614  VVATVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFL--DNDTYFK 673
            VVATVAFGMGL++ DV AV+H  +P S E Y+Q +GRAGRDG+ ++CHLFL    +   +
Sbjct: 777  VVATVAFGMGLDRPDVRAVLHLGLPPSFESYVQAVGRAGRDGQPAHCHLFLQPQGEDLRE 836

Query: 674  LRSLMHSDGVDEYNINKFISEVFSENKC--------------------------GKVYSM 733
            LR  +H+D  D   + + +  VF    C                           +  S 
Sbjct: 837  LRRHVHADSTDFLAVKRLVQRVFPACTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSH 896

Query: 734  VKDPASRK-----------------FDMKEEVMITILTYLELGEMQYLRLLPQLNVTCTL 793
               P  R+                  DM EE + T+L YLEL    +L LL      C L
Sbjct: 897  QAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRL 956

Query: 794  NFHKTSPAML-------ADKDIIVAEILKKSETKQGQHV-FDMLTVVNSIGISATSLSNH 853
            N     PA L           + +A+ L +   +    V FDM+ +V+S+G    S+   
Sbjct: 957  NC-PGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRA 1016

Query: 854  L---------------------------QNLKLKGEITYEMKD----------------- 881
            L                            +L+  G++T E KD                 
Sbjct: 1017 LCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQA 1076

BLAST of Cp4.1LG09g06170 vs. ExPASy Swiss-Prot
Match: Q75NR7 (ATP-dependent DNA helicase Q4 OS=Mus musculus OX=10090 GN=Recql4 PE=2 SV=2)

HSP 1 Score: 251.1 bits (640), Expect = 4.6e-65
Identity = 210/729 (28.81%), Postives = 321/729 (44.03%), Query Frame = 0

Query: 314  GYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVL----PGITVVVSPLVAL 373
            GY +F+ GQ   I  +L+G ST++VLPTGAGKS+CYQ+PA++     P +T+VVSPL++L
Sbjct: 494  GYRAFRPGQERAIMRILSGISTLLVLPTGAGKSLCYQLPALLYAQRSPCLTLVVSPLLSL 553

Query: 374  MIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERF-----QNPDFLS 433
            M DQ+  LP  ++   L S     +   +++ +    + VL VSPE       + P  L 
Sbjct: 554  MDDQVSDLPSCLKAACLHSGMTKKQRESVLKKVRAAQVHVLIVSPEALVGCGARGPGSLP 613

Query: 434  MFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCILAMTA 493
              +    ++   +DE HC+S+W        SHNFRP Y+R+   +LR  + + C L +TA
Sbjct: 614  QAAQLPPIAFACIDEVHCLSQW--------SHNFRPCYLRV-CKVLREHMGVRCFLGLTA 673

Query: 494  TATTSTMQAIMTALEIPSD-NLILKTTVRTNLQLV--------KDLLKLIKSSPIAEVRS 553
            TAT ST + +   L I  +  L     +  NL L         + L+ L++      + S
Sbjct: 674  TATRSTARDVAQHLGIAGEFELSGSANIPANLHLSVSMDRDSDQALVTLLQGDRFRTLDS 733

Query: 554  IIIYCK---------------FQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFC 613
            +IIYC                  +  DS  R  C     A++YH+G+ +++R+ +Q  F 
Sbjct: 734  VIIYCTRERIQNGWLALLRTCLSMVGDSRPRG-CGPEAIAEAYHAGMSSQERRRVQQAFM 793

Query: 614  SNKIRVVVATVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFL--D 673
               +R+VVATVAFGMGL++ DV AV+H  +P S E Y+Q IGRAGRDG+ ++CHLF+   
Sbjct: 794  RGHLRMVVATVAFGMGLDRPDVRAVLHLGLPPSFESYVQAIGRAGRDGKPAHCHLFMHPQ 853

Query: 674  NDTYFKLRSLMHSDGVDEYNINKFISEVFSENKCGKVYSMVKDPASRK------------ 733
             +  ++LR   H+D  D   + + +  VF    C +       P   K            
Sbjct: 854  GEDLWELRRHAHADSTDFLAVKRLVQRVFPPCTCSQRPVSKSSPEEVKEHSGQQTYPVLG 913

Query: 734  ------------------FDMKEEVMITILTYLELGEMQYLRLLPQLNVTCTLNFHKTSP 793
                               DM EE + T+L YLEL    +L LLP     C L+    S 
Sbjct: 914  QACLGHERALPVQSTVQALDMTEEAIETLLCYLELHPRHWLELLPWTYAQCHLHCLGGSA 973

Query: 794  AM--LADKDIIVAEILKK---SETKQGQH--VFDMLTVVNSIGISATSLSNHLQNLK--- 853
             +  LA +   +A    K    +T QG+    F ++ + +S+G    S+   L  LK   
Sbjct: 974  QLQALAHRCPPLAACQAKWPPKDTSQGRSSLEFGVVELADSMGWKLASVRQALHQLKWDP 1033

Query: 854  ------------------------LKGEITYEMKD-----------------LAYCYTIL 881
                                     +G++T E KD                 LA+ + + 
Sbjct: 1034 EPKKGAAQGTGVLVKFSELAFHLHSRGDLTDEEKDQICDFLYNRVQAREHKALAHLHQMS 1093

BLAST of Cp4.1LG09g06170 vs. ExPASy Swiss-Prot
Match: Q9VGI8 (Bloom syndrome protein homolog OS=Drosophila melanogaster OX=7227 GN=Blm PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 2.1e-57
Identity = 136/370 (36.76%), Postives = 213/370 (57.57%), Query Frame = 0

Query: 309  LKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGITVVVSPLVA 368
            L  ++G  SF+  QL+ I   L G    V++PTG GKS+CYQ+PA++  G+T+V+SPL +
Sbjct: 729  LSYSFGLKSFRPNQLQVINATLLGNDCFVLMPTGGGKSLCYQLPAILTEGVTIVISPLKS 788

Query: 369  LMIDQIKQLPPV-IQGGLLCSSQRPDEVSEIVRLLIQ--GSMKVLFVSPERFQN----PD 428
            L+ DQI +L  + I    L   Q+  +V  I R L      +K+L+V+PE+  +     D
Sbjct: 789  LIFDQINKLASLDICAKSLSGEQKMADVMAIYRDLESQPPMVKLLYVTPEKISSSARFQD 848

Query: 429  FLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCILA 488
             L   +S   +S  V+DEAHC+S+W         H+FRP Y +L   +L+ +      +A
Sbjct: 849  TLDTLNSNNYISRFVIDEAHCVSQW--------GHDFRPDYKKL--GVLKKRFPNVPTIA 908

Query: 489  MTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQ----------LVKDLLKLIKSSPIA 548
            +TATAT      I+  L + +    L +  R+NL+           + D+ + I+S P  
Sbjct: 909  LTATATPRVRLDILAQLNLKNCKWFLSSFNRSNLRYRVLPKKGVSTLDDISRYIRSKP-- 968

Query: 549  EVRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATV 608
            +  S IIYC  + E D  S+ +C +G+ A SYH+GL   DR+  Q ++ + K+RV+ ATV
Sbjct: 969  QHFSGIIYCLSRKECDETSKKMCKDGVRAVSYHAGLTDTDRESRQKDWLTGKMRVICATV 1028

Query: 609  AFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHS 662
            AFGMG++K DV  V+HYS+P+S+E Y QE GRAGRDG ++ C L+ +     +++ ++ S
Sbjct: 1029 AFGMGIDKPDVRFVLHYSLPKSIEGYYQEAGRAGRDGDVADCILYYNYSDMLRIKKMLDS 1086

BLAST of Cp4.1LG09g06170 vs. ExPASy Swiss-Prot
Match: Q9CL21 (ATP-dependent DNA helicase RecQ OS=Pasteurella multocida (strain Pm70) OX=272843 GN=recQ PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.0e-56
Identity = 132/356 (37.08%), Postives = 208/356 (58.43%), Query Frame = 0

Query: 300 PSDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGI 359
           P     + +L   +GY SF++GQ E I   L GK ++V++ TG GKS+CYQIPA+   G+
Sbjct: 21  PLKQTALDVLHAVFGYQSFRKGQEEVIDATLMGKDSLVIMATGNGKSLCYQIPALCFEGL 80

Query: 360 TVVVSPLVALMIDQIKQ-LPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQ 419
           T+V+SPL++LM DQ+ Q L   I+   L SSQ   E  ++   L+ G++K+L+VSPE+  
Sbjct: 81  TLVISPLISLMKDQVDQLLANGIEADYLNSSQTFTEQQQVQNKLMSGTLKLLYVSPEKVM 140

Query: 420 NPDFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINC 479
              F  + S    VS + +DEAHCIS+W         H+FRP Y +L    L++      
Sbjct: 141 TTSFFHLISH-CKVSFVAIDEAHCISQW--------GHDFRPEYTQLGG--LKSCFPHAP 200

Query: 480 ILAMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQLV-----KDLLKLIKSSPIAEV 539
           I+A+TATA  +T Q I+  L + S ++ + +  R N++       K + +L +     + 
Sbjct: 201 IMALTATADHATRQDILRHLNLQSPHVYIGSFDRPNIRYTLVEKFKPMEQLCRFVLGQKG 260

Query: 540 RSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVAF 599
           +S IIYC  + + + ++  L + G+SA++YH+GL    R+ +Q  F  + ++VVVAT+AF
Sbjct: 261 KSGIIYCNSRSKVERIAESLRNKGVSAQAYHAGLETSQREQVQRAFQRDNVQVVVATIAF 320

Query: 600 GMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLM 650
           GMG+NK +V  V+H+ +P S+E Y QE GRAGRD   +   LF +   Y  L  ++
Sbjct: 321 GMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPAEAVLFYEPADYAWLHKIL 365

BLAST of Cp4.1LG09g06170 vs. NCBI nr
Match: XP_023542981.1 (ATP-dependent DNA helicase Q-like 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1654 bits (4284), Expect = 0.0
Identity = 881/963 (91.48%), Postives = 881/963 (91.48%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP
Sbjct: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT
Sbjct: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720
           GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL
Sbjct: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720

Query: 721 PQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780
           PQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN
Sbjct: 721 PQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780

Query: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV---------- 840
           HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV          
Sbjct: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKVRKLDAMFDAV 840

Query: 841 --------------------------------------------------------FLQS 889
                                                                   FLQS
Sbjct: 841 TSAVNLYEKKTQGCFNYEQTPCLEGKICSYFQEGDNYDIPNKMNPSSPFLRADIKVFLQS 900

BLAST of Cp4.1LG09g06170 vs. NCBI nr
Match: KAG6573663.1 (ATP-dependent DNA helicase Q-like 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1604 bits (4153), Expect = 0.0
Identity = 857/965 (88.81%), Postives = 867/965 (89.84%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPRDSNPPPPP   SSSVTPFTSA NAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRDSNPPPPP---SSSVTPFTSAFNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNF TSE+NR LSEPSQQNN ETEVA  STADWGSKDN F PGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFGTSEDNRPLSEPSQQNNVETEVAGCSTADWGSKDNSFIPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERS+YR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSTYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN +NEDC+ETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEET+SNVRNDP
Sbjct: 241 SHRRTKMNLKNEDCHETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETLSNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT
Sbjct: 301 SDDNLVQLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMT+LEIPSDNL+LKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTSLEIPSDNLVLKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIRVVVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRVVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENK--CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720
           GVDEYNINKFISEVFSEN   CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR
Sbjct: 661 GVDEYNINKFISEVFSENNNSCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720

Query: 721 LLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSL 780
           LLPQLNVTC LNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSL
Sbjct: 721 LLPQLNVTCILNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSL 780

Query: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV-------- 840
           SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWL+EIQTSKV        
Sbjct: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLSEIQTSKVRKLDAMFD 840

Query: 841 ----------------------------------------------------------FL 889
                                                                     FL
Sbjct: 841 AVTSAVNLCEKKTQGCFNYEQTPCLEGKICSYFQEGDNYDIPNKMNQSSPFLRADIKVFL 900

BLAST of Cp4.1LG09g06170 vs. NCBI nr
Match: XP_022966727.1 (ATP-dependent DNA helicase Q-like 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1599 bits (4141), Expect = 0.0
Identity = 857/965 (88.81%), Postives = 867/965 (89.84%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPR+S PPP     SSSVTPFTSASNAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRNSKPPP-----SSSVTPFTSASNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPY IRRASDQSRG+SSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYHIRRASDQSRGISSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSEEN SLSEPSQQNNAETEVAS STADWGSKDNLFSPGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEENPSLSEPSQQNNAETEVASCSTADWGSKDNLFSPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN +N DC+ETASF DEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP
Sbjct: 241 SHRRTKMNLKNGDCHETASFFDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQ+PAMVLPGIT
Sbjct: 301 SDDNLVELLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQMPAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIRVVVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRVVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENK--CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720
           GVDEYNINKFISEVFSEN   CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR
Sbjct: 661 GVDEYNINKFISEVFSENNNSCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720

Query: 721 LLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSL 780
           LLPQLNVTCTLNFHKTSPA+LADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGIS+TSL
Sbjct: 721 LLPQLNVTCTLNFHKTSPAVLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISSTSL 780

Query: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV-------- 840
           SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWL+EIQTSKV        
Sbjct: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLSEIQTSKVRKLDAMFD 840

Query: 841 ----------------------------------------------------------FL 889
                                                                     FL
Sbjct: 841 AVTSAVNLCEKKTQGCFNYEQTPCLEGKICSYFQEGDNYDIPNKMNQSSPFLRADIKVFL 900

BLAST of Cp4.1LG09g06170 vs. NCBI nr
Match: XP_022945198.1 (ATP-dependent DNA helicase Q-like 5 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1599 bits (4140), Expect = 0.0
Identity = 853/963 (88.58%), Postives = 862/963 (89.51%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPRDSNPPPPP   SSSVTPFTSA NAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRDSNPPPPP---SSSVTPFTSAFNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSE+NR LSE SQQNN ETEVA  S ADWGSKDN F PGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEDNRPLSELSQQNNVETEVAGCSMADWGSKDNSFIPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKF KKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFTKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN  NEDC+ETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEET+SNVRNDP
Sbjct: 241 SHRRTKMNLNNEDCHETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETLSNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQI AMVLPGIT
Sbjct: 301 SDDNLVQLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQISAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMT+LEIPSDNL+LKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTSLEIPSDNLVLKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIR VVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRAVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720
           GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL
Sbjct: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720

Query: 721 PQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780
           PQLNVTC LNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN
Sbjct: 721 PQLNVTCILNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780

Query: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV---------- 840
           HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCS+SAHLRKWL+EIQTSKV          
Sbjct: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSMSAHLRKWLSEIQTSKVRKLDAMFDAV 840

Query: 841 --------------------------------------------------------FLQS 889
                                                                   FLQS
Sbjct: 841 TSAVNLCEKKTQGCFNYEQTPCLEGKICSYFQEGDNYDIPNKMNQSSPFLRADIKVFLQS 900

BLAST of Cp4.1LG09g06170 vs. NCBI nr
Match: XP_022966728.1 (ATP-dependent DNA helicase Q-like 5 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1497 bits (3876), Expect = 0.0
Identity = 791/832 (95.07%), Postives = 801/832 (96.27%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPR+S PPP     SSSVTPFTSASNAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRNSKPPP-----SSSVTPFTSASNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPY IRRASDQSRG+SSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYHIRRASDQSRGISSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSEEN SLSEPSQQNNAETEVAS STADWGSKDNLFSPGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEENPSLSEPSQQNNAETEVASCSTADWGSKDNLFSPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN +N DC+ETASF DEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP
Sbjct: 241 SHRRTKMNLKNGDCHETASFFDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQ+PAMVLPGIT
Sbjct: 301 SDDNLVELLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQMPAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIRVVVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRVVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENK--CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720
           GVDEYNINKFISEVFSEN   CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR
Sbjct: 661 GVDEYNINKFISEVFSENNNSCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720

Query: 721 LLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSL 780
           LLPQLNVTCTLNFHKTSPA+LADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGIS+TSL
Sbjct: 721 LLPQLNVTCTLNFHKTSPAVLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISSTSL 780

Query: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV 822
           SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWL+EIQTSKV
Sbjct: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLSEIQTSKV 819

BLAST of Cp4.1LG09g06170 vs. ExPASy TrEMBL
Match: A0A6J1HNS2 (ATP-dependent DNA helicase Q-like 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466352 PE=3 SV=1)

HSP 1 Score: 1599 bits (4141), Expect = 0.0
Identity = 857/965 (88.81%), Postives = 867/965 (89.84%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPR+S PPP     SSSVTPFTSASNAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRNSKPPP-----SSSVTPFTSASNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPY IRRASDQSRG+SSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYHIRRASDQSRGISSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSEEN SLSEPSQQNNAETEVAS STADWGSKDNLFSPGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEENPSLSEPSQQNNAETEVASCSTADWGSKDNLFSPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN +N DC+ETASF DEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP
Sbjct: 241 SHRRTKMNLKNGDCHETASFFDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQ+PAMVLPGIT
Sbjct: 301 SDDNLVELLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQMPAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIRVVVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRVVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENK--CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720
           GVDEYNINKFISEVFSEN   CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR
Sbjct: 661 GVDEYNINKFISEVFSENNNSCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720

Query: 721 LLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSL 780
           LLPQLNVTCTLNFHKTSPA+LADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGIS+TSL
Sbjct: 721 LLPQLNVTCTLNFHKTSPAVLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISSTSL 780

Query: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV-------- 840
           SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWL+EIQTSKV        
Sbjct: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLSEIQTSKVRKLDAMFD 840

Query: 841 ----------------------------------------------------------FL 889
                                                                     FL
Sbjct: 841 AVTSAVNLCEKKTQGCFNYEQTPCLEGKICSYFQEGDNYDIPNKMNQSSPFLRADIKVFL 900

BLAST of Cp4.1LG09g06170 vs. ExPASy TrEMBL
Match: A0A6J1G056 (ATP-dependent DNA helicase Q-like 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449514 PE=3 SV=1)

HSP 1 Score: 1599 bits (4140), Expect = 0.0
Identity = 853/963 (88.58%), Postives = 862/963 (89.51%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPRDSNPPPPP   SSSVTPFTSA NAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRDSNPPPPP---SSSVTPFTSAFNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSE+NR LSE SQQNN ETEVA  S ADWGSKDN F PGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEDNRPLSELSQQNNVETEVAGCSMADWGSKDNSFIPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKF KKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFTKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN  NEDC+ETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEET+SNVRNDP
Sbjct: 241 SHRRTKMNLNNEDCHETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETLSNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQI AMVLPGIT
Sbjct: 301 SDDNLVQLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQISAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMT+LEIPSDNL+LKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTSLEIPSDNLVLKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIR VVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRAVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720
           GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL
Sbjct: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720

Query: 721 PQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780
           PQLNVTC LNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN
Sbjct: 721 PQLNVTCILNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780

Query: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV---------- 840
           HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCS+SAHLRKWL+EIQTSKV          
Sbjct: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSMSAHLRKWLSEIQTSKVRKLDAMFDAV 840

Query: 841 --------------------------------------------------------FLQS 889
                                                                   FLQS
Sbjct: 841 TSAVNLCEKKTQGCFNYEQTPCLEGKICSYFQEGDNYDIPNKMNQSSPFLRADIKVFLQS 900

BLAST of Cp4.1LG09g06170 vs. ExPASy TrEMBL
Match: A0A6J1HSY9 (ATP-dependent DNA helicase Q-like 5 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466352 PE=3 SV=1)

HSP 1 Score: 1497 bits (3876), Expect = 0.0
Identity = 791/832 (95.07%), Postives = 801/832 (96.27%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPR+S PPP     SSSVTPFTSASNAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRNSKPPP-----SSSVTPFTSASNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPY IRRASDQSRG+SSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYHIRRASDQSRGISSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSEEN SLSEPSQQNNAETEVAS STADWGSKDNLFSPGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEENPSLSEPSQQNNAETEVASCSTADWGSKDNLFSPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN +N DC+ETASF DEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP
Sbjct: 241 SHRRTKMNLKNGDCHETASFFDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQ+PAMVLPGIT
Sbjct: 301 SDDNLVELLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQMPAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIRVVVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRVVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENK--CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720
           GVDEYNINKFISEVFSEN   CGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR
Sbjct: 661 GVDEYNINKFISEVFSENNNSCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLR 720

Query: 721 LLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSL 780
           LLPQLNVTCTLNFHKTSPA+LADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGIS+TSL
Sbjct: 721 LLPQLNVTCTLNFHKTSPAVLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISSTSL 780

Query: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV 822
           SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWL+EIQTSKV
Sbjct: 781 SNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLSEIQTSKV 819

BLAST of Cp4.1LG09g06170 vs. ExPASy TrEMBL
Match: A0A6J1G092 (ATP-dependent DNA helicase Q-like 5 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449514 PE=3 SV=1)

HSP 1 Score: 1497 bits (3875), Expect = 0.0
Identity = 787/830 (94.82%), Postives = 796/830 (95.90%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           MNSDSDSDSDASHISATPPRDSNPPPPP   SSSVTPFTSA NAKCKFSKATSSLARTRS
Sbjct: 1   MNSDSDSDSDASHISATPPRDSNPPPPP---SSSVTPFTSAFNAKCKFSKATSSLARTRS 60

Query: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120
           KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA
Sbjct: 61  KPGKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPA 120

Query: 121 GFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGN 180
           GFFSKSSSFLKFRGSCLNFETSE+NR LSE SQQNN ETEVA  S ADWGSKDN F PGN
Sbjct: 121 GFFSKSSSFLKFRGSCLNFETSEDNRPLSELSQQNNVETEVAGCSMADWGSKDNSFIPGN 180

Query: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERSSYR 240
           PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKF KKFNRRKYGERSSYR
Sbjct: 181 PVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFTKKFNRRKYGERSSYR 240

Query: 241 SHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVRNDP 300
           SHRRTKMN  NEDC+ETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEET+SNVRNDP
Sbjct: 241 SHRRTKMNLNNEDCHETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETLSNVRNDP 300

Query: 301 SDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGIT 360
           SDDNLV+LLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQI AMVLPGIT
Sbjct: 301 SDDNLVQLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQISAMVLPGIT 360

Query: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420
           VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP
Sbjct: 361 VVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERFQNP 420

Query: 421 DFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCIL 480
           DFLSMFSSGLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLNINCIL
Sbjct: 421 DFLSMFSSGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNINCIL 480

Query: 481 AMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSPIAE 540
           AMTATATTSTMQAIMT+LEIPSDNL+LKTTVRTNLQL        VKDLLKLIKSSPIAE
Sbjct: 481 AMTATATTSTMQAIMTSLEIPSDNLVLKTTVRTNLQLSVSMSTNRVKDLLKLIKSSPIAE 540

Query: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 600
           VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKIR VVATVA
Sbjct: 541 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIRAVVATVA 600

Query: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660
           FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD
Sbjct: 601 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 660

Query: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720
           GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL
Sbjct: 661 GVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQYLRLL 720

Query: 721 PQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780
           PQLNVTC LNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN
Sbjct: 721 PQLNVTCILNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISATSLSN 780

Query: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV 822
           HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCS+SAHLRKWL+EIQTSKV
Sbjct: 781 HLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSMSAHLRKWLSEIQTSKV 819

BLAST of Cp4.1LG09g06170 vs. ExPASy TrEMBL
Match: A0A6J1DDG1 (ATP-dependent DNA helicase Q-like 5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019761 PE=3 SV=1)

HSP 1 Score: 1391 bits (3601), Expect = 0.0
Identity = 748/967 (77.35%), Postives = 804/967 (83.14%), Query Frame = 0

Query: 1   MNSDSDSDSDASHISATPPRDSNPPPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRS 60
           M+SDSDS SD SH+SATPPR  NPPPPP  S       TSASNA  +FSKA+SSLA  RS
Sbjct: 1   MDSDSDSGSDVSHVSATPPRTPNPPPPPSLS------LTSASNANRRFSKASSSLAHVRS 60

Query: 61  KPGKHSSR--VAKPSPPPDTKPSEEESLP-DWTPLPTLPYQIRRASDQSRGVSSSESIEM 120
           KP K SSR  VAK SP PD KPS++ES P DWTPLPTLP+QIRRASDQ R +SSSES+EM
Sbjct: 61  KPPKSSSRPRVAKRSPSPDAKPSKDESPPPDWTPLPTLPFQIRRASDQIRAISSSESMEM 120

Query: 121 LPAGFFSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFS 180
           LPAGFFSKSSSF KFR SCLN ETSE++RSLSEPSQQNN ETE+AS STADWG+KD LFS
Sbjct: 121 LPAGFFSKSSSFSKFRRSCLNLETSEDDRSLSEPSQQNNVETEIASCSTADWGTKDELFS 180

Query: 181 PGNPVKLVRKYPNLIGAHVPAPPVKVRKCGGEGNFVKLNLNGRKRKFIKKFNRRKYGERS 240
           P NPVK VRK+PNLIGA VP PPVKVRKCGGEGNFVKLN+NGRKRKF+KK + R YG+R 
Sbjct: 181 PANPVKFVRKHPNLIGARVPVPPVKVRKCGGEGNFVKLNMNGRKRKFVKKTSGRNYGQRG 240

Query: 241 SYRSHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVR 300
           S+R +RRTK   + EDC ETA  CDEDGLVTETTQ  QKQGNG AKFDP++IEETISNVR
Sbjct: 241 SFRPYRRTKTKLKTEDCLETAGLCDEDGLVTETTQQKQKQGNGRAKFDPITIEETISNVR 300

Query: 301 NDPSDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLP 360
           NDPS+DNLV+LLKL YGYDSFQ+GQLE IKMVLA KSTMVVLPTGAGKSICYQIPAM+LP
Sbjct: 301 NDPSNDNLVQLLKLVYGYDSFQDGQLEAIKMVLAKKSTMVVLPTGAGKSICYQIPAMILP 360

Query: 361 GITVVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERF 420
           GITVVVSPLVALMIDQIKQLPPVIQGG LCSSQRP+EVSEIVRLLIQG+MKVLFVSPERF
Sbjct: 361 GITVVVSPLVALMIDQIKQLPPVIQGGFLCSSQRPEEVSEIVRLLIQGTMKVLFVSPERF 420

Query: 421 QNPDFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNIN 480
           QN DFLS+FS GLVVSLLVVDEAHCISEW        SHNFRPSYMRLRASLLRAQLN+N
Sbjct: 421 QNTDFLSIFSGGLVVSLLVVDEAHCISEW--------SHNFRPSYMRLRASLLRAQLNVN 480

Query: 481 CILAMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL--------VKDLLKLIKSSP 540
           CILAMTATA T+T+QAIMTALEI SDNLILK+TVRTNLQL        VKDLL LIKSSP
Sbjct: 481 CILAMTATANTATIQAIMTALEIHSDNLILKSTVRTNLQLSVSMSKNRVKDLLMLIKSSP 540

Query: 541 IAEVRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVA 600
           I EV+SIIIYCKFQ ETD +SRYLCDNGISAKSYHSGLPAKDRKHIQ+NFCSNKI+VV+A
Sbjct: 541 ITEVQSIIIYCKFQSETDLISRYLCDNGISAKSYHSGLPAKDRKHIQENFCSNKIKVVIA 600

Query: 601 TVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLM 660
           TVAFGMGL+KRDVGAVIHYSMPESLEEY+QEIGRAGRDGRLSYCHLFLDNDTYFKLRSLM
Sbjct: 601 TVAFGMGLDKRDVGAVIHYSMPESLEEYVQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLM 660

Query: 661 HSDGVDEYNINKFISEVFSE-NKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEMQY 720
           HSDGVDEYNINKF+SEVF++ N CGKV S+VK+PASRKFD+KEEVMITILTYLELGE+QY
Sbjct: 661 HSDGVDEYNINKFLSEVFTDKNLCGKVCSIVKEPASRKFDIKEEVMITILTYLELGEIQY 720

Query: 721 LRLLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGISAT 780
           LRLLPQLNVTC LNFHKTSPAMLA+KD +VAEILKKSETKQGQHVFDM+TVVNSIGISAT
Sbjct: 721 LRLLPQLNVTCILNFHKTSPAMLAEKDTVVAEILKKSETKQGQHVFDMITVVNSIGISAT 780

Query: 781 SLSNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTSKV------ 840
           SLSNHLQNLKLKGE+TYEMKDLAYCYTILK PEDFCSLSAHLRKWL+EIQTSKV      
Sbjct: 781 SLSNHLQNLKLKGEVTYEMKDLAYCYTILKIPEDFCSLSAHLRKWLSEIQTSKVRKLDAM 840

Query: 841 ------------------------------------------------------------ 889
                                                                       
Sbjct: 841 FDAVTSAVNSCEKKTHGCFNYQQTPCLEGKICSYFQQGDSYDIPNKMNQSSPFLRADIKV 900

BLAST of Cp4.1LG09g06170 vs. TAIR 10
Match: AT1G27880.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 817.0 bits (2109), Expect = 1.5e-236
Identity = 499/965 (51.71%), Postives = 616/965 (63.83%), Query Frame = 0

Query: 4   DSDSDSDASHISATPPRDSNP-PPPPLPSSSSVTPFTSASNAKCKFSKATSSLARTRSKP 63
           D DSDSD SH+SATPPRDS P  PP L S +   P  S         K TSS +R++ K 
Sbjct: 2   DFDSDSDGSHVSATPPRDSFPSSPPQLQSPAKHVPPVS--------RKMTSSSSRSKPKA 61

Query: 64  GKHSSRVAKPSPPPDTKPSEEESLPDWTPLPTLPYQIRRASDQSRGVSSSESIEMLPAGF 123
             H        PPP+  PS+E  +P  +P P  P      ++    +  S+     PA F
Sbjct: 62  PTH--------PPPN--PSQEAPVP--SPYPPPPPPSPLFTNLPFRICQSQ-----PARF 121

Query: 124 FSKSSSFLKFRGSCLNFETSEENRSLSEPSQQNNAETEVASSSTADWGSKDNLFSPGNPV 183
            S  SSF +    C               S+ +    E   S   D+  +  L     P 
Sbjct: 122 SSSVSSFSRL---C---------------SRASFTSVEKLKSDGVDFVPEPPLVEVIAPP 181

Query: 184 KLV-RKYPNLIGAHVPAPPVK---VRKCG-GEGNFVKLNLNGRKRKFIKKFNRRKYGERS 243
           K V RK PNLI   + +PPVK    R  G GEGNFVKLNLNG++ K      +     RS
Sbjct: 182 KSVRRKPPNLITDTITSPPVKPMVFRSNGNGEGNFVKLNLNGKRGKKFPSKYKGVSKSRS 241

Query: 244 SYRSHRRTKMNSRNEDCNETASFCDEDGLVTETTQHPQKQGNGGAKFDPVSIEETISNVR 303
           SY S R  +   +  D +  +   +E  L        QKQ    A     S+E+ I  V+
Sbjct: 242 SY-SFRGKRYKKKEADGDGESLLEEESDL--------QKQIEDEANGFISSVEDAILAVK 301

Query: 304 NDPSDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLP 363
            + SD+NL KLL L YGYDSF++GQL+ IKM+L G STM+VLPTGAGKS+CYQIPAM+LP
Sbjct: 302 TEASDENLTKLLNLVYGYDSFRDGQLQAIKMILGGSSTMLVLPTGAGKSLCYQIPAMILP 361

Query: 364 GITVVVSPLVALMIDQIKQLPPVIQGGLLCSSQRPDEVSEIVRLLIQGSMKVLFVSPERF 423
           GIT+VVSPLV+LMIDQ+K LP +I+GGLL SSQRP+E +E +R L +G +KVLFVSPER 
Sbjct: 362 GITLVVSPLVSLMIDQLKHLPSIIKGGLLSSSQRPEEATETLRKLKEGIIKVLFVSPERL 421

Query: 424 QNPDFLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNIN 483
            N +FLSMF   L VSL+VVDEAHC+SEW        SHNFRPSYMRL+AS+L ++L   
Sbjct: 422 LNVEFLSMFRMSLSVSLVVVDEAHCVSEW--------SHNFRPSYMRLKASMLFSELKAE 481

Query: 484 CILAMTATATTSTMQAIMTALEIPSDNLILKTTVRTNLQL---------VKDLLKLIKSS 543
           CILAMTATATT T+QA+M++LEIPS NLI K+ +R N +L         +KDLL L++S 
Sbjct: 482 CILAMTATATTMTLQAVMSSLEIPSTNLIQKSQLRDNFELSVSLSGANRMKDLLILMESP 541

Query: 544 PIAEVRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVV 603
           P  E+RSII+YCKFQ ETD +S+YL DN I+AK YHSGLPAKDR  IQ++FCSNKIRVVV
Sbjct: 542 PYKEIRSIIVYCKFQYETDMISKYLRDNNINAKGYHSGLPAKDRVRIQESFCSNKIRVVV 601

Query: 604 ATVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSL 663
           ATVAFGMGL+K DVGAVIH+S+P S+EEY+QEIGRAGRDGRLSYCHLF DNDTY KLRSL
Sbjct: 602 ATVAFGMGLDKGDVGAVIHFSVPGSMEEYVQEIGRAGRDGRLSYCHLFYDNDTYLKLRSL 661

Query: 664 MHSDGVDEYNINKFISEVFS--ENKCGKVYSMVKDPASRKFDMKEEVMITILTYLELGEM 723
            HSDGVDEY + KF++ VFS    +  K+ S+V + AS+KFDMKEEVM TILT+LELGE+
Sbjct: 662 AHSDGVDEYAVGKFLTHVFSTETKQHEKICSLVIESASQKFDMKEEVMQTILTHLELGEV 721

Query: 724 QYLRLLPQLNVTCTLNFHKTSPAMLADKDIIVAEILKKSETKQGQHVFDMLTVVNSIGIS 783
           QYLR+LPQLN+ CTLNFHK+SP  LA +  IVA ILKKS  KQG HVFD+  V +SI ++
Sbjct: 722 QYLRMLPQLNICCTLNFHKSSPNTLAARSAIVAAILKKSHVKQGLHVFDIPAVASSICVA 781

Query: 784 ATSLSNHLQNLKLKGEITYEMKDLAYCYTILKYPEDFCSLSAHLRKWLAEIQTS------ 843
            T +   +Q LK+KGE+TYE+KD A+CYTILK P++ CSLS+HL KWL EI++       
Sbjct: 782 TTDVLAEIQALKMKGEVTYELKDSAFCYTILKSPKEICSLSSHLTKWLTEIESCKVRKLD 841

Query: 844 -----------------------------------------------------------K 887
                                                                      K
Sbjct: 842 IMSSAAVAAISVSNTSELSSGAKQTRSLQSRIFDYFNGDEKCDSPSKATQNCAFLRADIK 901

BLAST of Cp4.1LG09g06170 vs. TAIR 10
Match: AT4G35740.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 210.3 bits (534), Expect = 6.3e-54
Identity = 142/387 (36.69%), Postives = 219/387 (56.59%), Query Frame = 0

Query: 283 KFDPVSIEETISNVRNDPSDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTG 342
           K  P+ ++   S+ +N    + LVKLL+  +G+  F+  QLE I+ V++G+    ++PTG
Sbjct: 2   KKSPLPVQNVQSSDKNVAGKEALVKLLRWHFGHADFRGKQLEAIQAVVSGRDCFCLMPTG 61

Query: 343 AGKSICYQIPAMVLPGITVVVSPLVALMIDQIKQLPPV-IQGGLLCSSQRPDEVSEIVRL 402
            GKSICYQIPA+  PGI +VVSPL+ALM +Q+  L    I    L S+Q     ++I   
Sbjct: 62  GGKSICYQIPALAKPGIVLVVSPLIALMENQVMALKEKGIAAEYLSSTQATHVKNKIHED 121

Query: 403 LIQG--SMKVLFVSPERFQNPDFL----SMFSSGLVVSLLVVDEAHCISEWQVDSQFEPS 462
           L  G  S+++L+V+PE      F+     + S GL ++L+ +DEAHCIS W         
Sbjct: 122 LDSGKPSVRLLYVTPELIATKGFMLKLRKLHSRGL-LNLIAIDEAHCISSW--------G 181

Query: 463 HNFRPSYMRLRASLLRAQLNINCILAMTATATTSTMQAIMTALEIPSDNLILKTTVRTNL 522
           H+FRPSY +L  S LR  L    +LA+TATA     + ++ +L + +  ++  +  R N+
Sbjct: 182 HDFRPSYRQL--STLRDSLADVPVLALTATAAPKVQKDVIDSLNLRNPLVLKSSFNRPNI 241

Query: 523 -----------QLVKDLLKLIKSSPIAEVRSIIIYCKFQLETDSVSRYLCDNGISAKSYH 582
                          DL  L+KS         IIYC  +   D +S +L   GIS+ +YH
Sbjct: 242 FYEVRYKDLLDNAYTDLGNLLKSCGNI---CAIIYCLERTTCDDLSVHLSSIGISSAAYH 301

Query: 583 SGLPAKDRKHIQDNFCSNKIRVVVATVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRA 642
           +GL +K R  + D++ S+K +++VATVAFGMG++K+DV  V H+++P+S+E + QE GRA
Sbjct: 302 AGLNSKMRSTVLDDWLSSKKQIIVATVAFGMGIDKKDVRMVCHFNIPKSMESFYQESGRA 361

Query: 643 GRDGRLSYCHLFLDNDTYFKLRSLMHS 652
           GRD   S   L+   D   K+  L+ +
Sbjct: 362 GRDQLPSRSVLYYGVDDRKKMEYLLRN 374

BLAST of Cp4.1LG09g06170 vs. TAIR 10
Match: AT1G10930.1 (DNA helicase (RECQl4A) )

HSP 1 Score: 200.7 bits (509), Expect = 5.0e-51
Identity = 131/364 (35.99%), Postives = 203/364 (55.77%), Query Frame = 0

Query: 310 KLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGITVVVSPLVAL 369
           K  +G  SF+  Q E I   ++G    V++PTG GKS+ YQ+PA++  GIT+V+SPLV+L
Sbjct: 446 KKVFGNHSFRPNQREIINATMSGSDVFVLMPTGGGKSLTYQLPALICGGITLVISPLVSL 505

Query: 370 MIDQIKQLPPV-IQGGLLCSSQRPDEVSEIVRLL--IQGSMKVLFVSPERFQNPDFL--- 429
           + DQI  L    I    L +     E  +I + L       K+L+V+PE+    D L   
Sbjct: 506 IQDQIMNLLQANIPAASLSAGMEWAEQLKIFQELNSEHSKYKLLYVTPEKVAKSDSLLRH 565

Query: 430 --SMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCILA 489
             ++ S GL ++  V+DEAHC+S+W         H+FRP Y  L   +L+ +     +LA
Sbjct: 566 LENLNSRGL-LARFVIDEAHCVSQW--------GHDFRPDYQSL--GILKQKFPNIPVLA 625

Query: 490 MTATATTSTMQAIMTALEIPSDNLILKTTVRTNL---------QLVKDLLKLIKSSPIAE 549
           +TATAT S  + ++ AL + +  +  ++  R NL         + ++D+ K IK +   E
Sbjct: 626 LTATATASVKEDVVQALGLVNCVVFRQSFNRPNLWYSVVPKTKKCLEDIDKFIKENHFDE 685

Query: 550 VRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVA 609
               IIYC  +++ + VS  L + G  A  YH  +  + R  IQ  +  ++I ++ ATVA
Sbjct: 686 CG--IIYCLSRMDCEKVSERLQEFGHKAAFYHGSMEPEQRAFIQTQWSKDEINIICATVA 745

Query: 610 FGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSD 657
           FGMG+NK DV  VIH+S+P+S+E Y QE GRAGRDG+ S C L+     Y +++ ++   
Sbjct: 746 FGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGQRSSCVLYYGYGDYIRVKHMISQG 796

BLAST of Cp4.1LG09g06170 vs. TAIR 10
Match: AT1G60930.1 (RECQ helicase L4B )

HSP 1 Score: 195.7 bits (496), Expect = 1.6e-49
Identity = 146/466 (31.33%), Postives = 240/466 (51.50%), Query Frame = 0

Query: 300 PSDDNLVKLLKLAYGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGI 359
           P   NL    KL +G  SF+  Q E I   ++G    V++PTG GKS+ YQ+PA++  GI
Sbjct: 452 PWTKNLEVYNKLVFGNHSFRPNQREIINATMSGCDVFVLMPTGGGKSLTYQLPALLCAGI 511

Query: 360 TVVVSPLVALMIDQIKQLPPV-IQGGLLCSSQRPDEVSEIVRLLI--QGSMKVLFVSPER 419
           T+V+SPLV+L+ DQI  L    I    L +     E  EI++ L   +   K+L+V+PE+
Sbjct: 512 TLVISPLVSLIQDQIMNLLQTNISAASLSAGMEWAEQLEILQELSSEKSKYKLLYVTPEK 571

Query: 420 FQNPD----FLSMFSSGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRA 479
               +     L + +S  +++  V+DEAHC+S+W         H+FRP Y  L   +L+ 
Sbjct: 572 VAKSESLLRHLEILNSRSLLARFVIDEAHCVSQW--------GHDFRPDYQGL--GVLKQ 631

Query: 480 QLNINCILAMTATATTSTMQAIMTALEIPSDNLILKTTVRTNL---------QLVKDLLK 539
           +     +LA+TATATTS  + ++ AL + +  +  ++  R NL         + ++D+ K
Sbjct: 632 KFPNIPMLALTATATTSVKEDVVQALGLVNCVVFRQSFNRPNLWYSVVPKTNKCLEDIDK 691

Query: 540 LIKSSPIAEVRSIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNK 599
            I+ +   E    IIYC  +++ + V+  L   G  A  YH  +    R  +Q  +  ++
Sbjct: 692 FIRENHFDECG--IIYCLSRMDCEKVTEALRVFGHKAAFYHGSMDPGKRAFVQKQWSKDE 751

Query: 600 IRVVVATVAFGMGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYF 659
           I ++ ATVAFGMG+NK DV  VIH+S+P+S+E Y QE GRAGRDG+ S C L+     Y 
Sbjct: 752 INIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGQRSSCVLYYSYTDYI 811

Query: 660 KLRSLMHSDGVDEYNINKFISEVFSENKCGKVYSMVKDPASRKFDMKEEVMITILTYLEL 719
           +++ ++   G+ +  +              K+    K  + R  +   E ++ +++Y E 
Sbjct: 812 RVKHMISQGGLGQGQM--------------KMGYNCKASSGRMLETNTENLLRMVSYCE- 871

Query: 720 GEMQYLRLLPQLNV-----------TCTLNFHKTSPAMLADKDIIV 739
            E+   R L  +++           TC    + +S  +L DKD+ V
Sbjct: 872 NEVDCRRFLQLVHLGEKFDSTNCKNTCD---NCSSSKILIDKDVTV 887

BLAST of Cp4.1LG09g06170 vs. TAIR 10
Match: AT1G31360.1 (RECQ helicase L2 )

HSP 1 Score: 191.0 bits (484), Expect = 4.0e-48
Identity = 126/378 (33.33%), Postives = 203/378 (53.70%), Query Frame = 0

Query: 313 YGYDSFQEGQLETIKMVLAGKSTMVVLPTGAGKSICYQIPAMVLPGITVVVSPLVALMID 372
           +G   ++  Q E I  ++ G+  +V++  G GKS+CYQ+PAM+  G T+VVSPL++L+ D
Sbjct: 85  FGISKYRANQKEIINAIMTGRDVLVIMAAGGGKSLCYQLPAMLRGGTTLVVSPLLSLIQD 144

Query: 373 QIKQLPPV-IQGGLLCSSQRPDEVSEIVRLLIQG--SMKVLFVSPERF-QNPDFLSMFS- 432
           Q+  L  + I   +L S+   +    + + L +G   +K+L+V+PE+  ++  F+S    
Sbjct: 145 QVMGLAALGISAYMLTSTSGKENEKFVYKALEKGEDDLKILYVTPEKVSKSKRFMSKLEK 204

Query: 433 --SGLVVSLLVVDEAHCISEWQVDSQFEPSHNFRPSYMRLRASLLRAQLNINCILAMTAT 492
             +   +SL+ +DEAHC S+W         H+FRP Y  L  S+L+ Q     ++A+TAT
Sbjct: 205 CHNAGRLSLISIDEAHCCSQW--------GHDFRPDYKNL--SILKTQFPKVPMVALTAT 264

Query: 493 ATTSTMQAIMTALEIPSDNLILKTTVRTNL-----------QLVKDLLKLIKSSPIAEVR 552
           AT      ++  L IP     + +  R NL           +LV D +        +   
Sbjct: 265 ATQKVQNDLIEMLHIPKCVKFVSSVNRPNLFYSVREKSAVGKLVVDEIAEFIRESYSNNE 324

Query: 553 SIIIYCKFQLETDSVSRYLCDNGISAKSYHSGLPAKDRKHIQDNFCSNKIRVVVATVAFG 612
           S I+YC  + E + ++  L + GISA  YH+ + A  R+ +   +  NK++V+V TVAFG
Sbjct: 325 SGIVYCFSRKECEQIAGDLRERGISADYYHADMDANMREKVHMRWSKNKLQVIVGTVAFG 384

Query: 613 MGLNKRDVGAVIHYSMPESLEEYIQEIGRAGRDGRLSYCHLFLDNDTYFKLRSLMHSDGV 672
           MG+NK DV  VIH+S+ +S+E Y QE GRAGRDG  S C LF  +    +  S++  +  
Sbjct: 385 MGINKPDVRFVIHHSLSKSMETYYQESGRAGRDGLPSECILFFRSADVPRQSSMVFYEYS 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WVW72.1e-23551.71ATP-dependent DNA helicase Q-like 5 OS=Arabidopsis thaliana OX=3702 GN=RECQL5 PE... [more]
O947612.0e-6528.18ATP-dependent DNA helicase Q4 OS=Homo sapiens OX=9606 GN=RECQL4 PE=1 SV=2[more]
Q75NR74.6e-6528.81ATP-dependent DNA helicase Q4 OS=Mus musculus OX=10090 GN=Recql4 PE=2 SV=2[more]
Q9VGI82.1e-5736.76Bloom syndrome protein homolog OS=Drosophila melanogaster OX=7227 GN=Blm PE=1 SV... [more]
Q9CL211.0e-5637.08ATP-dependent DNA helicase RecQ OS=Pasteurella multocida (strain Pm70) OX=272843... [more]
Match NameE-valueIdentityDescription
XP_023542981.10.091.48ATP-dependent DNA helicase Q-like 5 [Cucurbita pepo subsp. pepo][more]
KAG6573663.10.088.81ATP-dependent DNA helicase Q-like 5, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022966727.10.088.81ATP-dependent DNA helicase Q-like 5 isoform X1 [Cucurbita maxima][more]
XP_022945198.10.088.58ATP-dependent DNA helicase Q-like 5 isoform X1 [Cucurbita moschata][more]
XP_022966728.10.095.07ATP-dependent DNA helicase Q-like 5 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1HNS20.088.81ATP-dependent DNA helicase Q-like 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1G0560.088.58ATP-dependent DNA helicase Q-like 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HSY90.095.07ATP-dependent DNA helicase Q-like 5 isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1G0920.094.82ATP-dependent DNA helicase Q-like 5 isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1DDG10.077.35ATP-dependent DNA helicase Q-like 5 isoform X2 OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
AT1G27880.11.5e-23651.71DEAD/DEAH box RNA helicase family protein [more]
AT4G35740.16.3e-5436.69DEAD/DEAH box RNA helicase family protein [more]
AT1G10930.15.0e-5135.99DNA helicase (RECQl4A) [more]
AT1G60930.11.6e-4931.33RECQ helicase L4B [more]
AT1G31360.14.0e-4833.33RECQ helicase L2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 314..518
e-value: 1.0E-18
score: 78.2
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 326..504
score: 18.770727
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 547..628
e-value: 3.5E-27
score: 106.3
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 530..627
e-value: 2.0E-18
score: 66.7
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 517..683
score: 18.844444
IPR004589DNA helicase, ATP-dependent, RecQ typeTIGRFAMTIGR00614TIGR00614coord: 308..655
e-value: 1.8E-96
score: 321.7
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 321..491
e-value: 2.6E-13
score: 50.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 504..690
e-value: 8.9E-46
score: 157.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 288..503
e-value: 5.7E-60
score: 204.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 358..647
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..33
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..175
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..173
NoneNo IPR availablePANTHERPTHR13710DNA HELICASE RECQ FAMILY MEMBERcoord: 63..822
NoneNo IPR availablePANTHERPTHR13710:SF108ATP-DEPENDENT DNA HELICASE Q4coord: 63..822
IPR002464DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved sitePROSITEPS00690DEAH_ATP_HELICASEcoord: 433..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g06170.1Cp4.1LG09g06170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042631 cellular response to water deprivation
biological_process GO:0006268 DNA unwinding involved in DNA replication
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006310 DNA recombination
cellular_component GO:0005694 chromosome
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0043138 3'-5' DNA helicase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0009378 four-way junction helicase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004386 helicase activity