Cp4.1LG09g03980 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g03980 vs. ExPASy Swiss-Prot
Match: Q93Z04 (Probable pectate lyase 13 OS=Arabidopsis thaliana OX=3702 GN=PMR6 PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.5e-149
Identity = 274/487 (56.26%), Postives = 338/487 (69.40%), Query Frame = 0

Query: 10  IWMSGLFFLVASAS--FNLTLPHQHPDPDSVADELQRTVNASISRRK------ALSIESK 69
           I++  LFF + SA+   NLTLPHQHP PDSVA  + R+VN S++RR+      + S  S 
Sbjct: 10  IFLLCLFFTLLSATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSS 69

Query: 70  DQAFSCLTGNPIDDCWRC-DPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 129
             + SC TGNPIDDCWRC D +W+ NRQRLADC IGFG   +GGK GKIY+VTDSSD+NP
Sbjct: 70  SSSSSCRTGNPIDDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNP 129

Query: 130 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 189
            NPTPGTLR+ VIQ EPLWI+FS++M I+LK ELIINS+KT+DGRG+ VHITG GC+T+Q
Sbjct: 130 TNPTPGTLRYGVIQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQ 189

Query: 190 YVSNIIIHNIHVHHCVPS------------GNANISSRKIWIDHCSLSYCTDGLIDAIMG 249
           YV +IIIHN+H++ C PS            G +   S+KIW+DHCS+S+CTDGLIDA+MG
Sbjct: 190 YVQHIIIHNLHIYDCKPSAGFEKRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMG 249

Query: 250 STGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVV 309
           ST ITISN+YF+HHDEVMLLGHDD Y  D+GMQVTIAFNHFG+ LVQRMPRCR GYIHVV
Sbjct: 250 STAITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVV 309

Query: 310 NNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAET-EWTAWNWRTDGD 369
           NNDFT W+MYAIGGS  PTINSQGNRY AP +P+AKEVT+R+D+ +  EW+ WNWRT+GD
Sbjct: 310 NNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGD 369

Query: 370 ILVNGAFFVPSGAGLSTQYGKASSTEPKYSN--------------------GEGIVYPGF 429
           ++ NGAFFV SG G+S+ Y KASS +PK ++                      G  Y  +
Sbjct: 370 LMENGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPRDDQGQSGNSYSPY 429

Query: 430 SGSTGGG---NGGYGGGTVINNSPMYGNSGGGEDD--FYGMIFKGGGNRVSPPWSPPMIL 450
            G  GGG    G  GGG  +      G+S    DD   + MIF         P  P + L
Sbjct: 430 GGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDSNVFQMIFGS-----DAPSRPRLTL 489

BLAST of Cp4.1LG09g03980 vs. ExPASy Swiss-Prot
Match: Q9SCP2 (Probable pectate lyase 12 OS=Arabidopsis thaliana OX=3702 GN=At3g53190 PE=2 SV=2)

HSP 1 Score: 520.8 bits (1340), Expect = 1.6e-146
Identity = 269/454 (59.25%), Postives = 316/454 (69.60%), Query Frame = 0

Query: 9   LIWMSGLFFLVASASFNL----TLPHQHPDPDSVADELQRTVNASISRRKALSIESKDQA 68
           ++    LF LV    F++     L   HPDP+ VA ++Q  VNASI+RR+AL    +  +
Sbjct: 8   IVLFFSLFLLVPQMVFSMLNRTLLLIPHPDPELVAYQVQWKVNASITRRQALDTTDQAGS 67

Query: 69  FSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNPENPT 128
             C+TGNPIDDCW+CDPNW  NRQ LADCGIGFG+ A+GGKGG+ Y VTDSSD +  NP 
Sbjct: 68  TPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFYFVTDSSDDDAVNPK 127

Query: 129 PGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQYVSN 188
           PGTLR+ VIQ EPLWI+F ++M IKLK ELI NS+KT+DGRGANVHI GGGCIT+QYVSN
Sbjct: 128 PGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYVSN 187

Query: 189 IIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCTDGLI 248
           IIIHNIH+HHC  SGN N+                      S+ IWIDHCSLS C DGLI
Sbjct: 188 IIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIWIDHCSLSRCKDGLI 247

Query: 249 DAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHG 308
           DA+MGSTGITISN++FSHH+EVMLLGH D Y  DSGMQVTIAFNHFGE L+QRMPRCR G
Sbjct: 248 DAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHFGEKLIQRMPRCRRG 307

Query: 309 YIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTAWNWR 368
           YIHVVNNDFT WEMYAIGGS  PTINSQGNRY AP NP AKEVT+R++  + +W  WNWR
Sbjct: 308 YIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKRVETPDGDWKGWNWR 367

Query: 369 TDGDILVNGAFFVPSGAGLSTQYGKASSTEPKYSN--GEGIVYPGFSGSTGGGNGGYGGG 428
           ++GDILVNGAFFV SG G   +Y KA S EPK ++   +   + G  G  GG N   G  
Sbjct: 368 SEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGVLG-VGGRNNNLGMW 427

Query: 429 TVINNSPMYGNSGGGEDDFYGMIFKGGG--NRVS 434
           T   +    G SG    + Y     G G  NR+S
Sbjct: 428 TTTGSE---GTSGLDSYNDYTDEMSGAGSTNRLS 457

BLAST of Cp4.1LG09g03980 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 492.7 bits (1267), Expect = 4.7e-138
Identity = 230/367 (62.67%), Postives = 276/367 (75.20%), Query Frame = 0

Query: 33  PDPDSVADELQRTVNASISRRKALSIESKDQAFSCLTGNPIDDCWRCDPNWAANRQRLAD 92
           PDP+SV +E+ +++NAS++ R+ L         SC TGNPIDDCWRCDP+W  +RQRLAD
Sbjct: 28  PDPESVVEEVHKSINASVAGRRKLGY------LSCTTGNPIDDCWRCDPHWEQHRQRLAD 87

Query: 93  CGIGFGRDAMGGKGGKIYIVTDSSDSNPENPTPGTLRHAVIQFEPLWIIFSADMTIKLKY 152
           C IGFG++A+GG+ G+IY+VTDS + NP +P PGTLRHAV+Q EPLWIIF  DMTI+LK 
Sbjct: 88  CAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKE 147

Query: 153 ELIINSFKTIDGRGANVHITGGGCITIQYVSNIIIHNIHVHHCVPSGNANISS------- 212
           ELI+NSFKTIDGRGA+VHI+GG CITIQYV+NIIIH IH+H C   GNA + S       
Sbjct: 148 ELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGW 207

Query: 213 --------------RKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHD 272
                           +W+DHCS S C DGLIDAIMGST IT+SN++ +HHD+VMLLGH 
Sbjct: 208 RTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHS 267

Query: 273 DRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSARPTINSQ 332
           D Y +D  MQVTIAFNHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA PTINSQ
Sbjct: 268 DTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 327

Query: 333 GNRYVAPVNPNAKEVTRRLDAAETEWTAWNWRTDGDILVNGAFFVPSGAGLSTQYGKASS 379
           GNR++AP    +KEVT+  DA E+EW  WNWR+ GD+L+NGAFF PSG   S+ Y KASS
Sbjct: 328 GNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASS 387

BLAST of Cp4.1LG09g03980 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 4.4e-136
Identity = 229/366 (62.57%), Postives = 273/366 (74.59%), Query Frame = 0

Query: 34  DPDSVADELQRTVNASISRRKALSIESKDQAFSCLTGNPIDDCWRCDPNWAANRQRLADC 93
           DPD VADE+ + V  S   R A     K   FSC TGNPIDDCWRCD NW  NR+RLADC
Sbjct: 88  DPDMVADEVAKLVQMSEQNRTA---RRKLGFFSCGTGNPIDDCWRCDRNWHKNRKRLADC 147

Query: 94  GIGFGRDAMGGKGGKIYIVTDSSDSNPENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYE 153
           GIGFGR+A+GG+ G+ YIVTD +D +  NP PGTLRHAVIQ EPLWI+F  DM I+LK E
Sbjct: 148 GIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQE 207

Query: 154 LIINSFKTIDGRGANVHITGGGCITIQYVSNIIIHNIHVHHCVPSGNANI---------- 213
           LI+NSFKTID RG+NVHI  G CITIQ+++N+IIH +H+H C P+GNA +          
Sbjct: 208 LIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHFGWR 267

Query: 214 -----------SSRKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDD 273
                       S  IWIDH SLS+C DGL+DA+MGST IT+SN++F+HH+EVMLLGH D
Sbjct: 268 TMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFTHHNEVMLLGHSD 327

Query: 274 RYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSARPTINSQG 333
            Y +D  MQVTIA+NHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA PTINSQG
Sbjct: 328 SYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQG 387

Query: 334 NRYVAPVNPNAKEVTRRLDAAETEWTAWNWRTDGDILVNGAFFVPSGAGLSTQYGKASST 379
           NRY AP++  AKEVT+R++   +EW  WNWR++GD+L+NGAFF PSGAG S  YG+ASS 
Sbjct: 388 NRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSL 447

BLAST of Cp4.1LG09g03980 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 485.0 bits (1247), Expect = 9.7e-136
Identity = 224/370 (60.54%), Postives = 269/370 (72.70%), Query Frame = 0

Query: 30  HQHPDPDSVADELQRTVNASISRRKALSIESKDQAFSCLTGNPIDDCWRCDPNWAANRQR 89
           H   +PD VADE+      S+      +   K   F+C TGNPIDDCWRCDPNW  NR+R
Sbjct: 43  HAVTNPDEVADEVLALTEMSVRNH---TERRKLGYFTCGTGNPIDDCWRCDPNWHKNRKR 102

Query: 90  LADCGIGFGRDAMGGKGGKIYIVTDSSDSNPENPTPGTLRHAVIQFEPLWIIFSADMTIK 149
           LADCGIGFGR+A+GG+ G+ Y+VTD  D NP NP PGTLRHAVIQ  PLWI+F  DM I+
Sbjct: 103 LADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRPLWIVFKRDMVIQ 162

Query: 150 LKYELIINSFKTIDGRGANVHITGGGCITIQYVSNIIIHNIHVHHCVPSGNANISSRK-- 209
           LK ELI+NSFKTIDGRGANVHI  GGCITIQ+V+N+I+H +H+H C P+GNA + S +  
Sbjct: 163 LKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKPTGNAMVRSSETH 222

Query: 210 -------------------IWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLL 269
                              +WIDH SLS+C DGL+DA+MGST ITISN++ +HH+EVMLL
Sbjct: 223 FGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISNNHLTHHNEVMLL 282

Query: 270 GHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSARPTI 329
           GH D Y++D  MQVTIA+NHFG  L+QRMPRCRHGY HVVNND+T WEMYAIGGSA PTI
Sbjct: 283 GHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTI 342

Query: 330 NSQGNRYVAPVNPNAKEVTRRLDAAETEWTAWNWRTDGDILVNGAFFVPSGAGLSTQYGK 379
           NSQGNRY AP NP AKEVT+R+D   + W  WNWR++GD+L NGA+F  SGA  S  Y +
Sbjct: 343 NSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGSYAR 402

BLAST of Cp4.1LG09g03980 vs. NCBI nr
Match: XP_023543015.1 (probable pectate lyase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 462/502 (92.03%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES
Sbjct: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHCVPSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK                   YSNGE
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPRYSNGE 420

Query: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 462
           GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM
Sbjct: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 480

BLAST of Cp4.1LG09g03980 vs. NCBI nr
Match: KAG7012916.1 (putative pectate lyase 13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 461/502 (91.83%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES
Sbjct: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHCVPSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK                   YSNGE
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPRYSNGE 420

Query: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 462
           GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM
Sbjct: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 480

BLAST of Cp4.1LG09g03980 vs. NCBI nr
Match: XP_022945874.1 (probable pectate lyase 18 [Cucurbita moschata])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 460/502 (91.63%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES
Sbjct: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIIN+FKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINNFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHCVPSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK                   YSNGE
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPRYSNGE 420

Query: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 462
           GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM
Sbjct: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 480

BLAST of Cp4.1LG09g03980 vs. NCBI nr
Match: KAG6573852.1 (putative pectate lyase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 921 bits (2380), Expect = 0.0
Identity = 460/502 (91.63%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES
Sbjct: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIIN+FKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINNFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHCVPSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK                   YSNGE
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPRYSNGE 420

Query: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 462
           GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM
Sbjct: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 480

BLAST of Cp4.1LG09g03980 vs. NCBI nr
Match: XP_022966978.1 (probable pectate lyase 18 [Cucurbita maxima])

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 456/502 (90.84%), Postives = 457/502 (91.04%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSGLFFLVA ASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES
Sbjct: 1   MFPVLSILLIWMSGLFFLVALASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHCVPSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK                   +SNGE
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPRFSNGE 420

Query: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 462
           GIVYPGFSGS GGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKG GNRVSPPWSPPM
Sbjct: 421 GIVYPGFSGSNGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGDGNRVSPPWSPPM 480

BLAST of Cp4.1LG09g03980 vs. ExPASy TrEMBL
Match: A0A6J1G261 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111449983 PE=3 SV=1)

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 460/502 (91.63%), Postives = 462/502 (92.03%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES
Sbjct: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIIN+FKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINNFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHCVPSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK                   YSNGE
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPRYSNGE 420

Query: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 462
           GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM
Sbjct: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 480

BLAST of Cp4.1LG09g03980 vs. ExPASy TrEMBL
Match: A0A6J1HTT6 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111466524 PE=3 SV=1)

HSP 1 Score: 910 bits (2353), Expect = 0.0
Identity = 456/502 (90.84%), Postives = 457/502 (91.04%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSGLFFLVA ASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES
Sbjct: 1   MFPVLSILLIWMSGLFFLVALASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP
Sbjct: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
           ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHCVPSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCVPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK                   +SNGE
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKSVALINQLTMNAGVLGAPRFSNGE 420

Query: 421 GIVYPGFSGSTGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVSPPWSPPM 462
           GIVYPGFSGS GGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKG GNRVSPPWSPPM
Sbjct: 421 GIVYPGFSGSNGGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGDGNRVSPPWSPPM 480

BLAST of Cp4.1LG09g03980 vs. ExPASy TrEMBL
Match: A0A6J1CBN1 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111010036 PE=3 SV=1)

HSP 1 Score: 809 bits (2090), Expect = 2.52e-293
Identity = 411/505 (81.39%), Postives = 435/505 (86.14%), Query Frame = 0

Query: 1   MFPVLSILLIWMSGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIES 60
           MFPVLSILLIWMSG  F VASASFNLTLPHQHPDPDSVADELQR VNASISRR+ LS+ES
Sbjct: 1   MFPVLSILLIWMSGSVFNVASASFNLTLPHQHPDPDSVADELQRRVNASISRRQMLSLES 60

Query: 61  KDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 120
           KD+AFSCL+GNPIDDCWRCDPNWAANRQRLADCGIGFGRDA+GGKGGKIYIV+DSSDS+P
Sbjct: 61  KDEAFSCLSGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAVGGKGGKIYIVSDSSDSDP 120

Query: 121 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 180
            NPTPGTLRHAVIQFEPLWIIFSADMTI+LKYELI+NSFKTIDGRGANVHITGGGCITIQ
Sbjct: 121 ANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIVNSFKTIDGRGANVHITGGGCITIQ 180

Query: 181 YVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCT 240
           YVSNIIIHNIHVHHC PSGNANI                     SSRKIWIDHCSLSYCT
Sbjct: 181 YVSNIIIHNIHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYCT 240

Query: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300
           DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR
Sbjct: 241 DGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPR 300

Query: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTA 360
           CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRY AP++PNAKEVTRRLDAAET+W+A
Sbjct: 301 CRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYTAPLDPNAKEVTRRLDAAETDWSA 360

Query: 361 WNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNGE 420
           WNWRTDGDILVNGAFFVPSGAG+STQYGKASS EPK                   Y++G+
Sbjct: 361 WNWRTDGDILVNGAFFVPSGAGVSTQYGKASSVEPKSVALINQLTMNAGVLAAPRYNSGQ 420

Query: 421 GIVYPGFSGST-GGGNGGYGGGTVINNSPMYGNSGGGEDDFYGMIFKGGGNRVS--PPWS 462
           GIVYPGFSG+  G G G YGG TVINNSP+ GN GGGEDDFYGMIFK GG RVS  PP S
Sbjct: 421 GIVYPGFSGNNDGSGIGEYGGNTVINNSPIGGNGGGGEDDFYGMIFKAGGYRVSSLPPLS 480

BLAST of Cp4.1LG09g03980 vs. ExPASy TrEMBL
Match: A0A1S3BGT0 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489433 PE=3 SV=1)

HSP 1 Score: 778 bits (2009), Expect = 7.32e-281
Identity = 396/511 (77.50%), Postives = 422/511 (82.58%), Query Frame = 0

Query: 1   MFPVLSILLIWM-SGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSIE 60
           MFPVL+ L IWM + +  + +S+SFNLTLPHQHPDPDSVADELQRTVNASI RR+ LSIE
Sbjct: 1   MFPVLTTLFIWMFASISCVSSSSSFNLTLPHQHPDPDSVADELQRTVNASIWRRQMLSIE 60

Query: 61  SKDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSN 120
           SKDQ FSCLTGNPIDDCWRCDPNW+ANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSD +
Sbjct: 61  SKDQTFSCLTGNPIDDCWRCDPNWSANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDLD 120

Query: 121 PENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITI 180
           P NPTPGTLRHAVIQFEPLWIIFSADMTI+LKYELIINSFKTIDGRGANVHITGGGCITI
Sbjct: 121 PANPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCITI 180

Query: 181 QYVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYC 240
           QYVSN+IIHN+HVHHC PSGNANI                     SSRKIWIDHCSLSYC
Sbjct: 181 QYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSYC 240

Query: 241 TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP 300
           TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP
Sbjct: 241 TDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMP 300

Query: 301 RCRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWT 360
           RCRHGYIHVVNNDFTAW+MYAIGGSA+PTINSQGNRY APV+PNAKEVTRRLDAAETEW 
Sbjct: 301 RCRHGYIHVVNNDFTAWQMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEWA 360

Query: 361 AWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSNG 420
            WNWRTDGDILVNGAFFVPSGAGLSTQYGKASS EPK                   Y+ G
Sbjct: 361 GWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPRYTGG 420

Query: 421 EGIVYPGFSGSTGG-GNGGYGGGTVINNSPMYGNSGGGE------DDFYGMIFKGGGNRV 462
           EGIVYPGFSG  GG G+ GYGG TVINNSP+Y N GGG       DD++GMIFK  GNR+
Sbjct: 421 EGIVYPGFSGGNGGNGDEGYGGNTVINNSPIYENGGGGNGNSNGNDDYFGMIFKANGNRI 480

BLAST of Cp4.1LG09g03980 vs. ExPASy TrEMBL
Match: A0A0A0KRG9 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G622520 PE=3 SV=1)

HSP 1 Score: 776 bits (2003), Expect = 6.96e-280
Identity = 396/510 (77.65%), Postives = 421/510 (82.55%), Query Frame = 0

Query: 1   MFP-VLSILLIWM-SGLFFLVASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSI 60
           MFP VL+ L IWM +     V+S+SFNLTLPHQHPDPDSVADELQRTVNAS+ RR+ LSI
Sbjct: 1   MFPRVLTTLFIWMVASSISCVSSSSFNLTLPHQHPDPDSVADELQRTVNASVWRRQMLSI 60

Query: 61  ESKDQAFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDS 120
           ESKDQ FSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSD 
Sbjct: 61  ESKDQTFSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDL 120

Query: 121 NPENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCIT 180
           +P NPTPGTLRHAVIQFEPLWIIFSADMTI+LKYELIINSFKTIDGRGANVHITGGGCIT
Sbjct: 121 DPTNPTPGTLRHAVIQFEPLWIIFSADMTIRLKYELIINSFKTIDGRGANVHITGGGCIT 180

Query: 181 IQYVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSY 240
           IQYVSN+IIHN+HVHHC PSGNANI                     SSRKIWIDHCSLSY
Sbjct: 181 IQYVSNVIIHNVHVHHCKPSGNANIRSTPTHVGHRGLSDGDGISIFSSRKIWIDHCSLSY 240

Query: 241 CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM 300
           CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM
Sbjct: 241 CTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRM 300

Query: 301 PRCRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEW 360
           PRCRHGYIHVVNNDFTAW+MYAIGGSA+PTINSQGNRY APV+PNAKEVTRRLDAAETEW
Sbjct: 301 PRCRHGYIHVVNNDFTAWQMYAIGGSAQPTINSQGNRYTAPVDPNAKEVTRRLDAAETEW 360

Query: 361 TAWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPK-------------------YSN 420
             WNWRTDGDILVNGAFFVPSGAGLSTQYGKASS EPK                   Y+ 
Sbjct: 361 AGWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSVEPKSVALINQLTMNAGVLGAPRYNG 420

Query: 421 GEGIVYPGFSGSTGG-GNGGYGGGTVINNSPMYGNSGG--------GEDDFYGMIFKGGG 458
           GEGIVYPGFSG +GG G+ GYGG TVINNSP+Y N GG        G DD++GMIFK  G
Sbjct: 421 GEGIVYPGFSGGSGGNGDEGYGGNTVINNSPIYENGGGSGGYGNGNGNDDYFGMIFKANG 480

BLAST of Cp4.1LG09g03980 vs. TAIR 10
Match: AT5G04310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 559.3 bits (1440), Expect = 2.9e-159
Identity = 282/495 (56.97%), Postives = 344/495 (69.49%), Query Frame = 0

Query: 7   ILLIWMSGLFFL--------VASASFNLTLPHQHPDPDSVADELQRTVNASISRRKALSI 66
           I +IW   L  L         +++ FNL+LPHQHP P+ V   +QR +N S+SRR+ L+ 
Sbjct: 17  ICIIWFCLLVSLSHHGRASSTSASIFNLSLPHQHPFPEHVVLNVQRKLNDSLSRRQLLTY 76

Query: 67  ESKDQAF------SCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIV 126
           +  D         SC+TGNPIDDCWRCDPNW+ANRQRLADC IGFG+  +GGKGG+ Y+V
Sbjct: 77  QQDDGTTASSPIPSCITGNPIDDCWRCDPNWSANRQRLADCSIGFGQGTLGGKGGQFYLV 136

Query: 127 TDSSDSNPENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHIT 186
           TDSSD++  NP PGTLRHAVIQ EPLWIIFS+DM IKLK+ELII S+KTIDGRG N+ IT
Sbjct: 137 TDSSDNDAANPIPGTLRHAVIQPEPLWIIFSSDMGIKLKHELIIGSYKTIDGRGTNIQIT 196

Query: 187 GGGCITIQYVSNIIIHNIHVHHCVPSGNANI---------------------SSRKIWID 246
           G GC+TIQ VS++IIHN+H+HHC PSGN  +                     +S  IW+D
Sbjct: 197 GHGCLTIQQVSHVIIHNVHIHHCKPSGNTLVASSPTHVGFRGVSDGDGISVSASHHIWVD 256

Query: 247 HCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGE 306
           HCSL YC DGLID I+ ST +TISN+YFSHHDEVMLLGHDDRY  D GMQVTIAFNHFGE
Sbjct: 257 HCSLGYCADGLIDVILASTAVTISNNYFSHHDEVMLLGHDDRYTADKGMQVTIAFNHFGE 316

Query: 307 ALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLD 366
            LVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSA PTINSQGNRY AP++PNAKEVT+R+D
Sbjct: 317 GLVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSASPTINSQGNRYTAPIDPNAKEVTKRVD 376

Query: 367 AAETEWTAWNWRTDGDILVNGAFFVPSGAGLSTQYGKASSTEPKYSN--GEGIVYPGFSG 426
           + E  W+ WNWRT+GD++VNGAFFVPSG G+S  Y +A+S +PK +    +  V  G  G
Sbjct: 377 SNEKHWSGWNWRTEGDVMVNGAFFVPSGDGVSPAYARATSVQPKAAAIIDQLTVNAGVFG 436

Query: 427 STGGGNG--------GYGGGTVINNSPMYGNSGGG-----EDDFYGMIFKGGGNRVSPPW 452
              G NG          GGGT+       G +GGG     +D  + +IF  G N  +   
Sbjct: 437 DPSGRNGQGGSFPGITNGGGTITRGYSKSGPAGGGSGSDSDDGLFTLIF--GNNSGAVAL 496

BLAST of Cp4.1LG09g03980 vs. TAIR 10
Match: AT3G54920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 530.8 bits (1366), Expect = 1.1e-150
Identity = 274/487 (56.26%), Postives = 338/487 (69.40%), Query Frame = 0

Query: 10  IWMSGLFFLVASAS--FNLTLPHQHPDPDSVADELQRTVNASISRRK------ALSIESK 69
           I++  LFF + SA+   NLTLPHQHP PDSVA  + R+VN S++RR+      + S  S 
Sbjct: 10  IFLLCLFFTLLSATKPLNLTLPHQHPSPDSVALHVIRSVNESLARRQLSSPSSSSSSSSS 69

Query: 70  DQAFSCLTGNPIDDCWRC-DPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNP 129
             + SC TGNPIDDCWRC D +W+ NRQRLADC IGFG   +GGK GKIY+VTDSSD+NP
Sbjct: 70  SSSSSCRTGNPIDDCWRCSDADWSTNRQRLADCSIGFGHGTLGGKNGKIYVVTDSSDNNP 129

Query: 130 ENPTPGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQ 189
            NPTPGTLR+ VIQ EPLWI+FS++M I+LK ELIINS+KT+DGRG+ VHITG GC+T+Q
Sbjct: 130 TNPTPGTLRYGVIQEEPLWIVFSSNMLIRLKQELIINSYKTLDGRGSAVHITGNGCLTLQ 189

Query: 190 YVSNIIIHNIHVHHCVPS------------GNANISSRKIWIDHCSLSYCTDGLIDAIMG 249
           YV +IIIHN+H++ C PS            G +   S+KIW+DHCS+S+CTDGLIDA+MG
Sbjct: 190 YVQHIIIHNLHIYDCKPSAGFEKRGRSDGDGISIFGSQKIWVDHCSMSHCTDGLIDAVMG 249

Query: 250 STGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVV 309
           ST ITISN+YF+HHDEVMLLGHDD Y  D+GMQVTIAFNHFG+ LVQRMPRCR GYIHVV
Sbjct: 250 STAITISNNYFTHHDEVMLLGHDDNYAPDTGMQVTIAFNHFGQGLVQRMPRCRRGYIHVV 309

Query: 310 NNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAET-EWTAWNWRTDGD 369
           NNDFT W+MYAIGGS  PTINSQGNRY AP +P+AKEVT+R+D+ +  EW+ WNWRT+GD
Sbjct: 310 NNDFTEWKMYAIGGSGNPTINSQGNRYSAPSDPSAKEVTKRVDSKDDGEWSNWNWRTEGD 369

Query: 370 ILVNGAFFVPSGAGLSTQYGKASSTEPKYSN--------------------GEGIVYPGF 429
           ++ NGAFFV SG G+S+ Y KASS +PK ++                      G  Y  +
Sbjct: 370 LMENGAFFVASGEGMSSMYSKASSVDPKAASLVDQLTRNAGVFGGPRDDQGQSGNSYSPY 429

Query: 430 SGSTGGG---NGGYGGGTVINNSPMYGNSGGGEDD--FYGMIFKGGGNRVSPPWSPPMIL 450
            G  GGG    G  GGG  +      G+S    DD   + MIF         P  P + L
Sbjct: 430 GGDGGGGGSSGGSSGGGMDVMGGTTRGSSSSSGDDSNVFQMIFGS-----DAPSRPRLTL 489

BLAST of Cp4.1LG09g03980 vs. TAIR 10
Match: AT3G53190.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-147
Identity = 269/454 (59.25%), Postives = 316/454 (69.60%), Query Frame = 0

Query: 9   LIWMSGLFFLVASASFNL----TLPHQHPDPDSVADELQRTVNASISRRKALSIESKDQA 68
           ++    LF LV    F++     L   HPDP+ VA ++Q  VNASI+RR+AL    +  +
Sbjct: 8   IVLFFSLFLLVPQMVFSMLNRTLLLIPHPDPELVAYQVQWKVNASITRRQALDTTDQAGS 67

Query: 69  FSCLTGNPIDDCWRCDPNWAANRQRLADCGIGFGRDAMGGKGGKIYIVTDSSDSNPENPT 128
             C+TGNPIDDCW+CDPNW  NRQ LADCGIGFG+ A+GGKGG+ Y VTDSSD +  NP 
Sbjct: 68  TPCITGNPIDDCWKCDPNWPNNRQGLADCGIGFGQYALGGKGGQFYFVTDSSDDDAVNPK 127

Query: 129 PGTLRHAVIQFEPLWIIFSADMTIKLKYELIINSFKTIDGRGANVHITGGGCITIQYVSN 188
           PGTLR+ VIQ EPLWI+F ++M IKLK ELI NS+KT+DGRGANVHI GGGCIT+QYVSN
Sbjct: 128 PGTLRYGVIQEEPLWIVFPSNMMIKLKQELIFNSYKTLDGRGANVHIVGGGCITLQYVSN 187

Query: 189 IIIHNIHVHHCVPSGNANI---------------------SSRKIWIDHCSLSYCTDGLI 248
           IIIHNIH+HHC  SGN N+                      S+ IWIDHCSLS C DGLI
Sbjct: 188 IIIHNIHIHHCYQSGNTNVRSSPTHYGFRTKSDGDGISIFGSKDIWIDHCSLSRCKDGLI 247

Query: 249 DAIMGSTGITISNSYFSHHDEVMLLGHDDRYVQDSGMQVTIAFNHFGEALVQRMPRCRHG 308
           DA+MGSTGITISN++FSHH+EVMLLGH D Y  DSGMQVTIAFNHFGE L+QRMPRCR G
Sbjct: 248 DAVMGSTGITISNNFFSHHNEVMLLGHSDHYEPDSGMQVTIAFNHFGEKLIQRMPRCRRG 307

Query: 309 YIHVVNNDFTAWEMYAIGGSARPTINSQGNRYVAPVNPNAKEVTRRLDAAETEWTAWNWR 368
           YIHVVNNDFT WEMYAIGGS  PTINSQGNRY AP NP AKEVT+R++  + +W  WNWR
Sbjct: 308 YIHVVNNDFTQWEMYAIGGSGNPTINSQGNRYTAPTNPFAKEVTKRVETPDGDWKGWNWR 367

Query: 369 TDGDILVNGAFFVPSGAGLSTQYGKASSTEPKYSN--GEGIVYPGFSGSTGGGNGGYGGG 428
           ++GDILVNGAFFV SG G   +Y KA S EPK ++   +   + G  G  GG N   G  
Sbjct: 368 SEGDILVNGAFFVASGEGAEMRYEKAYSVEPKSASFITQITFHSGVLG-VGGRNNNLGMW 427

Query: 429 TVINNSPMYGNSGGGEDDFYGMIFKGGG--NRVS 434
           T   +    G SG    + Y     G G  NR+S
Sbjct: 428 TTTGSE---GTSGLDSYNDYTDEMSGAGSTNRLS 457

BLAST of Cp4.1LG09g03980 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 492.7 bits (1267), Expect = 3.3e-139
Identity = 230/367 (62.67%), Postives = 276/367 (75.20%), Query Frame = 0

Query: 33  PDPDSVADELQRTVNASISRRKALSIESKDQAFSCLTGNPIDDCWRCDPNWAANRQRLAD 92
           PDP+SV +E+ +++NAS++ R+ L         SC TGNPIDDCWRCDP+W  +RQRLAD
Sbjct: 28  PDPESVVEEVHKSINASVAGRRKLGY------LSCTTGNPIDDCWRCDPHWEQHRQRLAD 87

Query: 93  CGIGFGRDAMGGKGGKIYIVTDSSDSNPENPTPGTLRHAVIQFEPLWIIFSADMTIKLKY 152
           C IGFG++A+GG+ G+IY+VTDS + NP +P PGTLRHAV+Q EPLWIIF  DMTI+LK 
Sbjct: 88  CAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKE 147

Query: 153 ELIINSFKTIDGRGANVHITGGGCITIQYVSNIIIHNIHVHHCVPSGNANISS------- 212
           ELI+NSFKTIDGRGA+VHI+GG CITIQYV+NIIIH IH+H C   GNA + S       
Sbjct: 148 ELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGW 207

Query: 213 --------------RKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHD 272
                           +W+DHCS S C DGLIDAIMGST IT+SN++ +HHD+VMLLGH 
Sbjct: 208 RTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHS 267

Query: 273 DRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSARPTINSQ 332
           D Y +D  MQVTIAFNHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA PTINSQ
Sbjct: 268 DTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 327

Query: 333 GNRYVAPVNPNAKEVTRRLDAAETEWTAWNWRTDGDILVNGAFFVPSGAGLSTQYGKASS 379
           GNR++AP    +KEVT+  DA E+EW  WNWR+ GD+L+NGAFF PSG   S+ Y KASS
Sbjct: 328 GNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASS 387

BLAST of Cp4.1LG09g03980 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 492.7 bits (1267), Expect = 3.3e-139
Identity = 230/367 (62.67%), Postives = 276/367 (75.20%), Query Frame = 0

Query: 33  PDPDSVADELQRTVNASISRRKALSIESKDQAFSCLTGNPIDDCWRCDPNWAANRQRLAD 92
           PDP+SV +E+ +++NAS++ R+ L         SC TGNPIDDCWRCDP+W  +RQRLAD
Sbjct: 28  PDPESVVEEVHKSINASVAGRRKLGY------LSCTTGNPIDDCWRCDPHWEQHRQRLAD 87

Query: 93  CGIGFGRDAMGGKGGKIYIVTDSSDSNPENPTPGTLRHAVIQFEPLWIIFSADMTIKLKY 152
           C IGFG++A+GG+ G+IY+VTDS + NP +P PGTLRHAV+Q EPLWIIF  DMTI+LK 
Sbjct: 88  CAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEPLWIIFQRDMTIQLKE 147

Query: 153 ELIINSFKTIDGRGANVHITGGGCITIQYVSNIIIHNIHVHHCVPSGNANISS------- 212
           ELI+NSFKTIDGRGA+VHI+GG CITIQYV+NIIIH IH+H C   GNA + S       
Sbjct: 148 ELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQGGNAMVRSSPRHFGW 207

Query: 213 --------------RKIWIDHCSLSYCTDGLIDAIMGSTGITISNSYFSHHDEVMLLGHD 272
                           +W+DHCS S C DGLIDAIMGST IT+SN++ +HHD+VMLLGH 
Sbjct: 208 RTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSNNHMTHHDKVMLLGHS 267

Query: 273 DRYVQDSGMQVTIAFNHFGEALVQRMPRCRHGYIHVVNNDFTAWEMYAIGGSARPTINSQ 332
           D Y +D  MQVTIAFNHFGE LVQRMPRCRHGY HVVNND+T WEMYAIGGSA PTINSQ
Sbjct: 268 DTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQ 327

Query: 333 GNRYVAPVNPNAKEVTRRLDAAETEWTAWNWRTDGDILVNGAFFVPSGAGLSTQYGKASS 379
           GNR++AP    +KEVT+  DA E+EW  WNWR+ GD+L+NGAFF PSG   S+ Y KASS
Sbjct: 328 GNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFTPSGGAASSSYAKASS 387