Cp4.1LG09g01230 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g01230 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 554/747 (74.16%), Postives = 648/747 (86.75%), Query Frame = 0

Query: 27  KTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPEREGQGNGGGEDRIIYSYQNVFHGV 86
           KTYV+ MD+SAMP  +++H +WYS+ ++SV     + + Q   G  +RI+Y+YQ  FHG+
Sbjct: 35  KTYVIHMDKSAMPLPYTNHLQWYSSKINSVT----QHKSQEEEGNNNRILYTYQTAFHGL 94

Query: 87  AARLSEEEAERLEEEHGVLAVFPEVKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVVV 146
           AA+L++EEAERLEEE GV+AV PE +YELHTTRSP FLGLE  +S   W++++ DHDVVV
Sbjct: 95  AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVV 154

Query: 147 GVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTKQNCNRKIVGARVFYHGYEAATG 206
           GVLDTGIWPESESFND GMSPVPA W+G CETG+ F K+NCNRKIVGARVFY GYEAATG
Sbjct: 155 GVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATG 214

Query: 207 KFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGYAYGTARGMAPGARIAAYKVCWV 266
           K +++LEYKSPRD+DGHGTHTAATVAGSPV GANL G+AYGTARGMA  AR+AAYKVCWV
Sbjct: 215 KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWV 274

Query: 267 GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNG 326
           GGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGNG
Sbjct: 275 GGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNG 334

Query: 327 GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTITGVSLYRGRITIQENKQFPVVY 386
           GPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR  + +NKQ+P+VY
Sbjct: 335 GPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVY 394

Query: 387 MGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAA 446
           +G N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGGIGM+L+NTA 
Sbjct: 395 LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTAT 454

Query: 447 NGEELVADCHLLPAVAVGETEGKAIKQYALTNRKPTATLGLLGTRLGVKPSPVVAAFSSR 506
           NGEELVAD H+LPAVAVGE EGK IKQYA+T++K TA+L +LGTR+G+KPSPVVAAFSSR
Sbjct: 455 NGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSR 514

Query: 507 GPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVA 566
           GPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVA
Sbjct: 515 GPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVA 574

Query: 567 ALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHINPRKALDP 626
           ALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL DAS A+PSSPYDHGAGHI+P +A DP
Sbjct: 575 ALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDP 634

Query: 627 GLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNRSC-HRLLPNPGDLNYPAISAVFPEKT 686
           GLVY+I PQ+YFEFLCTQDL+PSQLKVF+KHSNR+C H L  NPG+LNYPAISA+FPE T
Sbjct: 635 GLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENT 694

Query: 687 TITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKIEPERLNFTRRYQKLSYRITFVTKKR 746
            + ++TL RTVTNVGP  SSY   VSPF GA+V ++P+ LNFT ++QKLSY +TF T+ R
Sbjct: 695 HVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFR 754

Query: 747 LSMPEFGGLIWKDGSHRVRSPIIITWL 773
           +  PEFGGL+WK  +H+VRSP+IITWL
Sbjct: 755 MKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Cp4.1LG09g01230 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 3.4e-234
Identity = 409/757 (54.03%), Postives = 527/757 (69.62%), Query Frame = 0

Query: 17  CLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPEREGQGNGGGEDRII 76
           C + SS+    TY+V M +S MP SF  H  WY + L S + D+ E            ++
Sbjct: 20  CHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS-ISDSAE------------LL 79

Query: 77  YSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRSPKFLGLEPADSNSAWS 136
           Y+Y+N  HG + RL++EEA+ L  + GV++V PE +YELHTTR+P FLGL+   ++  + 
Sbjct: 80  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD-LFP 139

Query: 137 QQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTKQNCNRKIVGARV 196
           +  +  DVVVGVLDTG+WPES+S++D G  P+P+ WKG CE G  FT   CNRK++GAR 
Sbjct: 140 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 199

Query: 197 FYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGYAYGTARGMAPGA 256
           F  GYE+  G  ++  E +SPRD DGHGTHT++T AGS V GA+LLGYA GTARGMAP A
Sbjct: 200 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 259

Query: 257 RIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMG 316
           R+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AME G
Sbjct: 260 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 319

Query: 317 VFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTITGVSLYRGRITI 376
           + VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LGNG+  TGVSL++G    
Sbjct: 320 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL- 379

Query: 377 QENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGG 436
             +K  P +Y G N+SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK AGG
Sbjct: 380 -PDKLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 439

Query: 437 IGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRKPTATLGLLGTRLGVKP 496
           +GMIL+NTAANGEELVAD HLLPA  VGE  G  I+ Y  T+  PTA++ +LGT +GVKP
Sbjct: 440 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 499

Query: 497 SPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTS 556
           SPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+SGTS
Sbjct: 500 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 559

Query: 557 MSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAG 616
           MSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+DHGAG
Sbjct: 560 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 619

Query: 617 HINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNRSCHRLLPNPGDLNYPA 676
           H++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+ +         +  DLNYP+
Sbjct: 620 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPS 679

Query: 677 ISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKIEPERLNFTRRYQKLSY 736
            +        + +    RTVT+VG A +    + S  TG  + +EP  LNF    +K SY
Sbjct: 680 FAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSY 739

Query: 737 RITFV--TKKRLSMPEFGGLIWKDGSHRVRSPIIITW 772
            +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 740 TVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG09g01230 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 1.0e-222
Identity = 392/763 (51.38%), Postives = 519/763 (68.02%), Query Frame = 0

Query: 14  FFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPEREGQGNGGGED 73
           F   LL ++A+  KTY+++++ S  P+SF  H +WY++ L+S                E 
Sbjct: 17  FLFLLLHTTAK--KTYIIRVNHSDKPESFLTHHDWYTSQLNS----------------ES 76

Query: 74  RIIYSYQNVFHGVAARLSEEEAER-LEEEHGVLAVFPEVKYELHTTRSPKFLGLEPADSN 133
            ++Y+Y   FHG +A L   EA+  L   + +L +F +  Y LHTTR+P+FLGL      
Sbjct: 77  SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEF 136

Query: 134 SAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTKQNCNRKIV 193
                  + + V++GVLDTG+WPES SF+D  M  +P+ WKGECE+G  F  + CN+K++
Sbjct: 137 GVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLI 196

Query: 194 GARVFYHGYEAAT-GKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGYAYGTARG 253
           GAR F  G++ A+ G F+ + E  SPRD DGHGTHT+ T AGS V  A+ LGYA GTARG
Sbjct: 197 GARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARG 256

Query: 254 MAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFG 313
           MA  AR+A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF 
Sbjct: 257 MATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFS 316

Query: 314 AMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTITGVSLYR 373
           AME GVFVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LGNG+ +TGVSLY 
Sbjct: 317 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 376

Query: 374 GRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVV 433
           G       K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV
Sbjct: 377 G--VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVV 436

Query: 434 KNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRKPTATLGLLGTR 493
           ++AGG+GMI++NTAA+GEELVAD HLLPA+AVG+  G  +++Y  ++ KPTA L   GT 
Sbjct: 437 RDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTV 496

Query: 494 LGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNI 553
           L VKPSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI
Sbjct: 497 LDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNI 556

Query: 554 LSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPY 613
           +SGTSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL DA+  S S+PY
Sbjct: 557 MSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPY 616

Query: 614 DHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNRSCHRLLPNPGD 673
            HG+GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C +   +PG 
Sbjct: 617 AHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQ 676

Query: 674 LNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKIEPERLNFTRRY 733
           LNYP+ S +F  K  +      R VTNVG ASS Y   V+      + ++P +L+F    
Sbjct: 677 LNYPSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVG 736

Query: 734 QKLSYRITFVTKKRLSM---PEFGGLIWKDGSHRVRSPIIITW 772
           +K  Y +TFV+KK +SM    EFG + W +  H VRSP+  +W
Sbjct: 737 EKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Cp4.1LG09g01230 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 756.5 bits (1952), Expect = 2.9e-217
Identity = 388/781 (49.68%), Postives = 528/781 (67.61%), Query Frame = 0

Query: 11  FPLFFHCLLLS------SAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPERE 70
           F  FF  L LS      S+    TY+V +D  A P  F  HF WY++ L+S+    P   
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPS-- 63

Query: 71  GQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRSPKFL 130
                     II++Y  VFHG +ARL+ ++A +L +   V++V PE    LHTTRSP+FL
Sbjct: 64  ----------IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFL 123

Query: 131 GLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTK 190
           GL   D      +     D+V+GV+DTG+WPE  SF+D G+ PVP  WKG+C   + F +
Sbjct: 124 GLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPE 183

Query: 191 QNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGY 250
             CNRK+VGAR F  GYEA  GK N+  E++SPRD DGHGTHTA+  AG  V  A+ LGY
Sbjct: 184 SACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGY 243

Query: 251 AYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDS 310
           A+G A GMAP AR+AAYKVCW  GC+ SDIL+A D AVADGV+V+S+S+GG V  YY D+
Sbjct: 244 AHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDA 303

Query: 311 LSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTI 370
           +++ AFGA++ G+FVS SAGNGGP  +++TNV+PW+TTVGA T+DRDFPA VKLGNG+ I
Sbjct: 304 IAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMI 363

Query: 371 TGVSLYRGRITIQENKQFPVVYMGS-NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 430
           +GVS+Y G   +   + +P+VY GS    +   SSLCLEG+LDP+ V GKIV+CDRGI+ 
Sbjct: 364 SGVSVYGGP-GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINS 423

Query: 431 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQY------ALT 490
           R  KG +V+  GG+GMI++N   +GE LVADCH+LPA +VG + G  I++Y      + +
Sbjct: 424 RATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRS 483

Query: 491 NRKPTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGP 550
           ++ PTAT+   GTRLG++P+PVVA+FS+RGPN  T EILKPD++APG+NILAAW  + GP
Sbjct: 484 SKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 543

Query: 551 SSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYK 610
           S +T+D RR +FNILSGTSM+CPHVSG+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +
Sbjct: 544 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 603

Query: 611 PLKDASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQL-KVFSK 670
           P+ D S  + SS  D+G+GH++P KA+DPGLVY+I   DY  FLC  + T + +  +  +
Sbjct: 604 PMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRR 663

Query: 671 HSNRSCHRLLPNPGDLNYPAISAVFPEKTTITSLTLH--RTVTNVGPASSSYHAIVSPFT 730
            ++    R   + G+LNYP+ S VF ++   + ++ H  RTVTNVG + S Y   + P  
Sbjct: 664 QADCDGARRAGHVGNLNYPSFSVVF-QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPR 723

Query: 731 GAAVKIEPERLNFTRRYQKLSYRITFVTKKRLSMP-----EFGGLIWKDGSHRVRSPIII 771
           G  V +EPE+L+F R  QKLS+ +   T +    P     E G ++W DG   V SP+++
Sbjct: 724 GTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVV 770

BLAST of Cp4.1LG09g01230 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 2.0e-213
Identity = 390/786 (49.62%), Postives = 523/786 (66.54%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MA + +  L F L F  +  +++Q  KT++ ++D  +MP  F  H+ WYST  +      
Sbjct: 1   MASSTIVLLLF-LSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE----- 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
                      E RI++ Y  VFHG +A ++ +EA+ L     VLAVF + + ELHTTRS
Sbjct: 61  -----------ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           P+FLGL+  +    WS+     DV++GV DTGIWPE  SF+D  + P+P  W+G CE+G 
Sbjct: 121 PQFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGA 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAAT-GKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGA 240
            F+ +NCNRKI+GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     A
Sbjct: 181 RFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKA 240

Query: 241 NLLGYAYGTARGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG-- 300
           ++ GYA G A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG  
Sbjct: 241 SMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDG 300

Query: 301 -VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAI 360
             S YY D +++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA 
Sbjct: 301 ITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPAD 360

Query: 361 VKLGNGRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIV 420
             LG+G  + GVSLY G       + FPVVY G   S    +SLC+E TLDP  V GKIV
Sbjct: 361 AILGDGHRLRGVSLYAG--VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIV 420

Query: 421 ICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYA 480
           ICDRG SPRV KG+VVK AGG+GMIL+N A+NGE LV D HL+PA AVG  EG  IK YA
Sbjct: 421 ICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYA 480

Query: 481 LTNRKPTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 540
            ++  P A++   GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   
Sbjct: 481 SSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAV 540

Query: 541 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 600
           GP+ L +D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+
Sbjct: 541 GPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNS 600

Query: 601 YKPLKDASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFS 660
            + L D S    ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V +
Sbjct: 601 NRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT 660

Query: 661 KHSNRSCHRLLPNPGDLNYPAISAVFP-EKTTITSLTLHRTVTNVGPASSSYHAIVSPFT 720
           +   R      P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    
Sbjct: 661 RTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPR 720

Query: 721 GAAVKIEPERLNFTRRYQKLSYRITF-VTKKRLSMPE----FGGLIWKD-GSHRVRSPII 775
           G  V ++P RL FT   ++ SY +T  V  + + + E    FG + W D G H VRSPI+
Sbjct: 721 GVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIV 763

BLAST of Cp4.1LG09g01230 vs. NCBI nr
Match: XP_023542216.1 (subtilisin-like protease SBT1.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1565 bits (4051), Expect = 0.0
Identity = 775/775 (100.00%), Postives = 775/775 (100.00%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN
Sbjct: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775
           EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775

BLAST of Cp4.1LG09g01230 vs. NCBI nr
Match: KAG6574133.1 (Subtilisin-like protease 1.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1561 bits (4042), Expect = 0.0
Identity = 773/775 (99.74%), Postives = 774/775 (99.87%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDH EWYSTV+SSVVVDN
Sbjct: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775
           EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775

BLAST of Cp4.1LG09g01230 vs. NCBI nr
Match: XP_022945029.1 (subtilisin-like protease SBT1.3 [Cucurbita moschata])

HSP 1 Score: 1556 bits (4029), Expect = 0.0
Identity = 769/775 (99.23%), Postives = 772/775 (99.61%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPLQWLCFPLFFHCL  SSAQFFKTYVVQMDRSAMPDSFSDH EWYSTV+SSVVVDN
Sbjct: 1   MAHTPLQWLCFPLFFHCLFFSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           DASAASPSSPY+HGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR
Sbjct: 601 DASAASPSSPYEHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SCHRLLPNPGDLNYPAISAVFPEKTT+TSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775
           EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775

BLAST of Cp4.1LG09g01230 vs. NCBI nr
Match: XP_022967972.1 (subtilisin-like protease SBT1.3 [Cucurbita maxima])

HSP 1 Score: 1538 bits (3983), Expect = 0.0
Identity = 761/775 (98.19%), Postives = 768/775 (99.10%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPLQWLC PLFFHC LLSSAQFF TYVVQMDRSAMPDSFSD FEWYSTVLSSVVVDN
Sbjct: 1   MAHTPLQWLCLPLFFHCFLLSSAQFFNTYVVQMDRSAMPDSFSDPFEWYSTVLSSVVVDN 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAER+EEEHGVLAVFPEVKYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERVEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATL +LGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLEILGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLR 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SC+RLL NPGDLNYPAISAVFPEKTT+TSLTLHRTVTNVGPASSSYHAIVSPF GAAVK+
Sbjct: 661 SCNRLLLNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFNGAAVKV 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775
           EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775

BLAST of Cp4.1LG09g01230 vs. NCBI nr
Match: KAG7013190.1 (Subtilisin-like protease SBT1.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1536 bits (3977), Expect = 0.0
Identity = 760/763 (99.61%), Postives = 762/763 (99.87%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDH EWYSTV+SSVVVDN
Sbjct: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRV 763
           EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHR+
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRL 763

BLAST of Cp4.1LG09g01230 vs. ExPASy TrEMBL
Match: A0A6J1FZU3 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111449392 PE=3 SV=1)

HSP 1 Score: 1556 bits (4029), Expect = 0.0
Identity = 769/775 (99.23%), Postives = 772/775 (99.61%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPLQWLCFPLFFHCL  SSAQFFKTYVVQMDRSAMPDSFSDH EWYSTV+SSVVVDN
Sbjct: 1   MAHTPLQWLCFPLFFHCLFFSSAQFFKTYVVQMDRSAMPDSFSDHLEWYSTVVSSVVVDN 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           DASAASPSSPY+HGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR
Sbjct: 601 DASAASPSSPYEHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SCHRLLPNPGDLNYPAISAVFPEKTT+TSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI
Sbjct: 661 SCHRLLPNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775
           EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775

BLAST of Cp4.1LG09g01230 vs. ExPASy TrEMBL
Match: A0A6J1HS88 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111467351 PE=3 SV=1)

HSP 1 Score: 1538 bits (3983), Expect = 0.0
Identity = 761/775 (98.19%), Postives = 768/775 (99.10%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPLQWLC PLFFHC LLSSAQFF TYVVQMDRSAMPDSFSD FEWYSTVLSSVVVDN
Sbjct: 1   MAHTPLQWLCLPLFFHCFLLSSAQFFNTYVVQMDRSAMPDSFSDPFEWYSTVLSSVVVDN 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAER+EEEHGVLAVFPEVKYELHTTRS
Sbjct: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERVEEEHGVLAVFPEVKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR
Sbjct: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATL +LGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLEILGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPL+
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLR 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR
Sbjct: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SC+RLL NPGDLNYPAISAVFPEKTT+TSLTLHRTVTNVGPASSSYHAIVSPF GAAVK+
Sbjct: 661 SCNRLLLNPGDLNYPAISAVFPEKTTVTSLTLHRTVTNVGPASSSYHAIVSPFNGAAVKV 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775
           EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF
Sbjct: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLSSF 775

BLAST of Cp4.1LG09g01230 vs. ExPASy TrEMBL
Match: A0A6J1E4V2 (subtilisin-like protease SBT1.3 OS=Cucurbita moschata OX=3662 GN=LOC111430759 PE=3 SV=1)

HSP 1 Score: 1460 bits (3779), Expect = 0.0
Identity = 717/773 (92.76%), Postives = 748/773 (96.77%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPL+WLC  L F CLLLSSAQF KTYVVQMDRSAMPDSFSDH +WYSTVL+SVVV N
Sbjct: 1   MAHTPLKWLCLLLVFDCLLLSSAQFMKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVV-N 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREG  NGGGE RIIYSYQNVFHGVAARL+EEEAERLEEE+GVLA+FPE KYELHTTRS
Sbjct: 61  PEREG--NGGGEQRIIYSYQNVFHGVAARLNEEEAERLEEENGVLAIFPETKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPA+WKGECETGR
Sbjct: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
            FTKQNCNRKIVGAR+FYHGYEAATGKFN+QLEYKSPRDQDGHGTHTAATVAGSP AGAN
Sbjct: 181 DFTKQNCNRKIVGARMFYHGYEAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPTAGAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLY+GRITI E+KQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTVTGVSLYKGRITIPESKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHL+PAVAVGE EGKAIK YALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKLYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
           PTATLG LGTRLGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 PTATLGFLGTRLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           D+S ASPSSPYDHGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTPSQLKVFSK+SNR
Sbjct: 601 DSSGASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SCH LLPNPGDLNYPAISAVFPEKT++TSLTLHRTVTNVGPA+SSYHA+V+PF GAAVK+
Sbjct: 661 SCHHLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFNGAAVKV 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773
           EPE LNFTRRYQKLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPI+ITWLS
Sbjct: 721 EPESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLS 770

BLAST of Cp4.1LG09g01230 vs. ExPASy TrEMBL
Match: A0A6J1J576 (subtilisin-like protease SBT1.3 OS=Cucurbita maxima OX=3661 GN=LOC111482733 PE=3 SV=1)

HSP 1 Score: 1444 bits (3738), Expect = 0.0
Identity = 709/773 (91.72%), Postives = 741/773 (95.86%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MAHTPL+WLC  L F C LLSSAQF KTYVVQMDRSAMPDSFSDH +WYSTVL+SVVV N
Sbjct: 1   MAHTPLKWLCLLLVFDCSLLSSAQFLKTYVVQMDRSAMPDSFSDHLQWYSTVLTSVVV-N 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
           PEREG  NGGGE RIIYSYQNVFHGVAARLSEEEAERLEEE GVLA+FPE KY+LHTTRS
Sbjct: 61  PEREG--NGGGEQRIIYSYQNVFHGVAARLSEEEAERLEEEDGVLAIFPETKYDLHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDV+VGVLDTGIWPESESFNDAGMSPVPA+WKGECETGR
Sbjct: 121 PRFLGLEPADSNSAWSQQIADHDVIVGVLDTGIWPESESFNDAGMSPVPAHWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
            FTKQNCNRKIVGAR+FYHGYEAATGKFN++LEYKSPRDQDGHGTHTAATVAGSP A AN
Sbjct: 181 DFTKQNCNRKIVGARMFYHGYEAATGKFNEKLEYKSPRDQDGHGTHTAATVAGSPTASAN 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCW GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWAGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGN GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNAGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT+TGVSLY+GRITI ENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG
Sbjct: 361 GRTVTGVSLYKGRITIPENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGG+GMIL+NTAANGEELVADCHL+PAVAVGE EGKAIK YALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGVGMILANTAANGEELVADCHLVPAVAVGEREGKAIKVYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
            TA+LG LGT+LGV+PSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 ATASLGFLGTKLGVRPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           D+S  SPSSPYDHGAGHINPRKALDPGLVYEI+PQDYFEFLCTQDLTPSQLKVFSK+SNR
Sbjct: 601 DSSGTSPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFEFLCTQDLTPSQLKVFSKYSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           SCH LLPNPGDLNYPAISAVFPEK ++TSLTLHRTVTNVGPA+SSYHA+ +PF GAAVK+
Sbjct: 661 SCHHLLPNPGDLNYPAISAVFPEKASVTSLTLHRTVTNVGPATSSYHAVATPFNGAAVKV 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773
           EPE LNFTRRYQKLSY+ITF+TKKR SMPEFGGLIWKDG+H VRSPI+ITWLS
Sbjct: 721 EPESLNFTRRYQKLSYKITFLTKKRQSMPEFGGLIWKDGTHSVRSPIVITWLS 770

BLAST of Cp4.1LG09g01230 vs. ExPASy TrEMBL
Match: A0A5A7SUB4 (Subtilisin-like protease SBT1.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00040 PE=3 SV=1)

HSP 1 Score: 1430 bits (3702), Expect = 0.0
Identity = 705/773 (91.20%), Postives = 747/773 (96.64%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MA +P++ LC  LF   LLLSSA F KTYVVQMDRSAMP SF++HFEWYS VLS+VVVD 
Sbjct: 1   MADSPIKCLCLLLFLDSLLLSSALFLKTYVVQMDRSAMPASFTNHFEWYSNVLSNVVVDL 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
            EREG G GGGE+RIIYSYQNVFHGVAARLSEEE E+LEEE GV+A+FPE+KYELHTTRS
Sbjct: 61  -EREGNG-GGGEERIIYSYQNVFHGVAARLSEEEVEKLEEEDGVVAIFPEMKYELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           P+FLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPES+SF+DAGMSPVPA+WKGECETGR
Sbjct: 121 PRFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESDSFDDAGMSPVPAHWKGECETGR 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGAN 240
           GFTKQNCNRKIVGARVFYHGY+AATGKFN+QLEYKSPRDQDGHGTHTAATVAGSPVAGA+
Sbjct: 181 GFTKQNCNRKIVGARVFYHGYQAATGKFNEQLEYKSPRDQDGHGTHTAATVAGSPVAGAS 240

Query: 241 LLGYAYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300
           LLGYAYGTARGMAPGARIAAYKVCW+GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY
Sbjct: 241 LLGYAYGTARGMAPGARIAAYKVCWIGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSY 300

Query: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGN 360
           YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLG+
Sbjct: 301 YRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGD 360

Query: 361 GRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRG 420
           GRT++G SLYRGRITI ENKQFP+VYMGSNSS+PDPSSLCLEGTLDPH VAGKIVICDRG
Sbjct: 361 GRTMSGASLYRGRITIPENKQFPIVYMGSNSSSPDPSSLCLEGTLDPHVVAGKIVICDRG 420

Query: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRK 480
           ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHL+PAVAVGE EGKAIKQYALTNRK
Sbjct: 421 ISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLVPAVAVGEKEGKAIKQYALTNRK 480

Query: 481 PTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540
            TATLG LGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL
Sbjct: 481 ATATLGFLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSL 540

Query: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLK 600
           TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAY+HDNTYKPL+
Sbjct: 541 TTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYIHDNTYKPLQ 600

Query: 601 DASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNR 660
           D+SAASPSSPYDHGAGHINPRKALDPGLVYEI+PQDYF+FLCTQDLTP+QLKVFSK+SNR
Sbjct: 601 DSSAASPSSPYDHGAGHINPRKALDPGLVYEIQPQDYFDFLCTQDLTPTQLKVFSKYSNR 660

Query: 661 SCHRLLPNPGDLNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKI 720
           +C RLLPNPGDLNYPAISAVFPEKT++TSLTLHRTVTNVGPA+SSYHA+V+PF GAAVK+
Sbjct: 661 TCRRLLPNPGDLNYPAISAVFPEKTSVTSLTLHRTVTNVGPATSSYHAVVTPFKGAAVKV 720

Query: 721 EPERLNFTRRYQKLSYRITFVTKKRLSMPEFGGLIWKDGSHRVRSPIIITWLS 773
           EPE LNFTRRY+KLSYRITFVTKKRLSMPEFGGLIWKDGSH+VRSPI+ITWLS
Sbjct: 721 EPESLNFTRRYEKLSYRITFVTKKRLSMPEFGGLIWKDGSHKVRSPIVITWLS 771

BLAST of Cp4.1LG09g01230 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 554/747 (74.16%), Postives = 648/747 (86.75%), Query Frame = 0

Query: 27  KTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPEREGQGNGGGEDRIIYSYQNVFHGV 86
           KTYV+ MD+SAMP  +++H +WYS+ ++SV     + + Q   G  +RI+Y+YQ  FHG+
Sbjct: 35  KTYVIHMDKSAMPLPYTNHLQWYSSKINSVT----QHKSQEEEGNNNRILYTYQTAFHGL 94

Query: 87  AARLSEEEAERLEEEHGVLAVFPEVKYELHTTRSPKFLGLEPADSNSAWSQQIADHDVVV 146
           AA+L++EEAERLEEE GV+AV PE +YELHTTRSP FLGLE  +S   W++++ DHDVVV
Sbjct: 95  AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVV 154

Query: 147 GVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTKQNCNRKIVGARVFYHGYEAATG 206
           GVLDTGIWPESESFND GMSPVPA W+G CETG+ F K+NCNRKIVGARVFY GYEAATG
Sbjct: 155 GVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATG 214

Query: 207 KFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGYAYGTARGMAPGARIAAYKVCWV 266
           K +++LEYKSPRD+DGHGTHTAATVAGSPV GANL G+AYGTARGMA  AR+AAYKVCWV
Sbjct: 215 KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWV 274

Query: 267 GGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMGVFVSCSAGNG 326
           GGCFSSDILSAVD+AVADGV VLSISLGGGVS+Y RDSLS+A FGAMEMGVFVSCSAGNG
Sbjct: 275 GGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNG 334

Query: 327 GPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTITGVSLYRGRITIQENKQFPVVY 386
           GPDP+SLTNVSPWITTVGASTMDRDFPA VK+G  RT  GVSLY+GR  + +NKQ+P+VY
Sbjct: 335 GPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVY 394

Query: 387 MGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGGIGMILSNTAA 446
           +G N+S+PDP+S CL+G LD   VAGKIVICDRG++PRVQKG VVK AGGIGM+L+NTA 
Sbjct: 395 LGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTAT 454

Query: 447 NGEELVADCHLLPAVAVGETEGKAIKQYALTNRKPTATLGLLGTRLGVKPSPVVAAFSSR 506
           NGEELVAD H+LPAVAVGE EGK IKQYA+T++K TA+L +LGTR+G+KPSPVVAAFSSR
Sbjct: 455 NGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSR 514

Query: 507 GPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTSMSCPHVSGVA 566
           GPNFL+LEILKPDL+APGVNILAAWTG   PSSL++D RRVKFNILSGTSMSCPHVSGVA
Sbjct: 515 GPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVA 574

Query: 567 ALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAGHINPRKALDP 626
           ALIKS+HPDWSP+AIKSALMTTAYVHDN +KPL DAS A+PSSPYDHGAGHI+P +A DP
Sbjct: 575 ALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDP 634

Query: 627 GLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNRSC-HRLLPNPGDLNYPAISAVFPEKT 686
           GLVY+I PQ+YFEFLCTQDL+PSQLKVF+KHSNR+C H L  NPG+LNYPAISA+FPE T
Sbjct: 635 GLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENT 694

Query: 687 TITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKIEPERLNFTRRYQKLSYRITFVTKKR 746
            + ++TL RTVTNVGP  SSY   VSPF GA+V ++P+ LNFT ++QKLSY +TF T+ R
Sbjct: 695 HVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFR 754

Query: 747 LSMPEFGGLIWKDGSHRVRSPIIITWL 773
           +  PEFGGL+WK  +H+VRSP+IITWL
Sbjct: 755 MKRPEFGGLVWKSTTHKVRSPVIITWL 777

BLAST of Cp4.1LG09g01230 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 812.8 bits (2098), Expect = 2.4e-235
Identity = 409/757 (54.03%), Postives = 527/757 (69.62%), Query Frame = 0

Query: 17  CLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPEREGQGNGGGEDRII 76
           C + SS+    TY+V M +S MP SF  H  WY + L S + D+ E            ++
Sbjct: 20  CHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS-ISDSAE------------LL 79

Query: 77  YSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRSPKFLGLEPADSNSAWS 136
           Y+Y+N  HG + RL++EEA+ L  + GV++V PE +YELHTTR+P FLGL+   ++  + 
Sbjct: 80  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTAD-LFP 139

Query: 137 QQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTKQNCNRKIVGARV 196
           +  +  DVVVGVLDTG+WPES+S++D G  P+P+ WKG CE G  FT   CNRK++GAR 
Sbjct: 140 EAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARF 199

Query: 197 FYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGYAYGTARGMAPGA 256
           F  GYE+  G  ++  E +SPRD DGHGTHT++T AGS V GA+LLGYA GTARGMAP A
Sbjct: 200 FARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 259

Query: 257 RIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFGAMEMG 316
           R+A YKVCW+GGCFSSDIL+A+D+A+AD VNVLS+SLGGG+S YYRD +++ AF AME G
Sbjct: 260 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 319

Query: 317 VFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTITGVSLYRGRITI 376
           + VSCSAGN GP   SL+NV+PWITTVGA T+DRDFPA+  LGNG+  TGVSL++G    
Sbjct: 320 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL- 379

Query: 377 QENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVVKNAGG 436
             +K  P +Y G N+SN    +LC+ GTL P  V GKIV+CDRGI+ RVQKG VVK AGG
Sbjct: 380 -PDKLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 439

Query: 437 IGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRKPTATLGLLGTRLGVKP 496
           +GMIL+NTAANGEELVAD HLLPA  VGE  G  I+ Y  T+  PTA++ +LGT +GVKP
Sbjct: 440 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 499

Query: 497 SPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNILSGTS 556
           SPVVAAFSSRGPN +T  ILKPDL+APGVNILAAWTG  GP+ L +D+RRV+FNI+SGTS
Sbjct: 500 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 559

Query: 557 MSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPYDHGAG 616
           MSCPHVSG+AAL+KS HP+WSP+AI+SALMTTAY      KPL D +   PS+P+DHGAG
Sbjct: 560 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 619

Query: 617 HINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNRSCHRLLPNPGDLNYPA 676
           H++P  A +PGL+Y++  +DY  FLC  + T  Q++  S+ +         +  DLNYP+
Sbjct: 620 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPS 679

Query: 677 ISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKIEPERLNFTRRYQKLSY 736
            +        + +    RTVT+VG A +    + S  TG  + +EP  LNF    +K SY
Sbjct: 680 FAV---NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSY 739

Query: 737 RITFV--TKKRLSMPEFGGLIWKDGSHRVRSPIIITW 772
            +TF   + K      FG + W DG H V SP+ I+W
Sbjct: 740 TVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG09g01230 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 774.6 bits (1999), Expect = 7.3e-224
Identity = 392/763 (51.38%), Postives = 519/763 (68.02%), Query Frame = 0

Query: 14  FFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPEREGQGNGGGED 73
           F   LL ++A+  KTY+++++ S  P+SF  H +WY++ L+S                E 
Sbjct: 17  FLFLLLHTTAK--KTYIIRVNHSDKPESFLTHHDWYTSQLNS----------------ES 76

Query: 74  RIIYSYQNVFHGVAARLSEEEAER-LEEEHGVLAVFPEVKYELHTTRSPKFLGLEPADSN 133
            ++Y+Y   FHG +A L   EA+  L   + +L +F +  Y LHTTR+P+FLGL      
Sbjct: 77  SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN--SEF 136

Query: 134 SAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTKQNCNRKIV 193
                  + + V++GVLDTG+WPES SF+D  M  +P+ WKGECE+G  F  + CN+K++
Sbjct: 137 GVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLI 196

Query: 194 GARVFYHGYEAAT-GKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGYAYGTARG 253
           GAR F  G++ A+ G F+ + E  SPRD DGHGTHT+ T AGS V  A+ LGYA GTARG
Sbjct: 197 GARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARG 256

Query: 254 MAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDSLSVAAFG 313
           MA  AR+A YKVCW  GCF SDIL+A+DRA+ DGV+VLS+SLGGG + YYRD++++ AF 
Sbjct: 257 MATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFS 316

Query: 314 AMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTITGVSLYR 373
           AME GVFVSCSAGN GP   S+ NV+PW+ TVGA T+DRDFPA   LGNG+ +TGVSLY 
Sbjct: 317 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 376

Query: 374 GRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISPRVQKGVVV 433
           G       K   +VY   NSS+   S+LCL G+LD   V GKIV+CDRG++ RV+KG VV
Sbjct: 377 G--VGMGTKPLELVYNKGNSSS---SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVV 436

Query: 434 KNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYALTNRKPTATLGLLGTR 493
           ++AGG+GMI++NTAA+GEELVAD HLLPA+AVG+  G  +++Y  ++ KPTA L   GT 
Sbjct: 437 RDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTV 496

Query: 494 LGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGPSSLTTDTRRVKFNI 553
           L VKPSPVVAAFSSRGPN +T EILKPD++ PGVNILA W+   GP+ L  D+RR +FNI
Sbjct: 497 LDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNI 556

Query: 554 LSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYKPLKDASAASPSSPY 613
           +SGTSMSCPH+SG+A L+K+ HP+WSPSAIKSALMTTAYV DNT  PL DA+  S S+PY
Sbjct: 557 MSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPY 616

Query: 614 DHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFSKHSNRSCHRLLPNPGD 673
            HG+GH++P+KAL PGLVY+I  ++Y  FLC+ D T   +    K  + +C +   +PG 
Sbjct: 617 AHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQ 676

Query: 674 LNYPAISAVFPEKTTITSLTLHRTVTNVGPASSSYHAIVSPFTGAAVKIEPERLNFTRRY 733
           LNYP+ S +F  K  +      R VTNVG ASS Y   V+      + ++P +L+F    
Sbjct: 677 LNYPSFSVLFGGKRVV---RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVG 736

Query: 734 QKLSYRITFVTKKRLSM---PEFGGLIWKDGSHRVRSPIIITW 772
           +K  Y +TFV+KK +SM    EFG + W +  H VRSP+  +W
Sbjct: 737 EKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Cp4.1LG09g01230 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 756.5 bits (1952), Expect = 2.1e-218
Identity = 388/781 (49.68%), Postives = 528/781 (67.61%), Query Frame = 0

Query: 11  FPLFFHCLLLS------SAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDNPERE 70
           F  FF  L LS      S+    TY+V +D  A P  F  HF WY++ L+S+    P   
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPS-- 63

Query: 71  GQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRSPKFL 130
                     II++Y  VFHG +ARL+ ++A +L +   V++V PE    LHTTRSP+FL
Sbjct: 64  ----------IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFL 123

Query: 131 GLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGRGFTK 190
           GL   D      +     D+V+GV+DTG+WPE  SF+D G+ PVP  WKG+C   + F +
Sbjct: 124 GLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPE 183

Query: 191 QNCNRKIVGARVFYHGYEAATGKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGANLLGY 250
             CNRK+VGAR F  GYEA  GK N+  E++SPRD DGHGTHTA+  AG  V  A+ LGY
Sbjct: 184 SACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGY 243

Query: 251 AYGTARGMAPGARIAAYKVCWVGGCFSSDILSAVDRAVADGVNVLSISLGGGVSSYYRDS 310
           A+G A GMAP AR+AAYKVCW  GC+ SDIL+A D AVADGV+V+S+S+GG V  YY D+
Sbjct: 244 AHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDA 303

Query: 311 LSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAIVKLGNGRTI 370
           +++ AFGA++ G+FVS SAGNGGP  +++TNV+PW+TTVGA T+DRDFPA VKLGNG+ I
Sbjct: 304 IAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMI 363

Query: 371 TGVSLYRGRITIQENKQFPVVYMGS-NSSNPDPSSLCLEGTLDPHFVAGKIVICDRGISP 430
           +GVS+Y G   +   + +P+VY GS    +   SSLCLEG+LDP+ V GKIV+CDRGI+ 
Sbjct: 364 SGVSVYGGP-GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINS 423

Query: 431 RVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQY------ALT 490
           R  KG +V+  GG+GMI++N   +GE LVADCH+LPA +VG + G  I++Y      + +
Sbjct: 424 RATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRS 483

Query: 491 NRKPTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKTGP 550
           ++ PTAT+   GTRLG++P+PVVA+FS+RGPN  T EILKPD++APG+NILAAW  + GP
Sbjct: 484 SKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 543

Query: 551 SSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNTYK 610
           S +T+D RR +FNILSGTSM+CPHVSG+AAL+K+ HPDWSP+AI+SAL+TTAY  DN+ +
Sbjct: 544 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 603

Query: 611 PLKDASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQL-KVFSK 670
           P+ D S  + SS  D+G+GH++P KA+DPGLVY+I   DY  FLC  + T + +  +  +
Sbjct: 604 PMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRR 663

Query: 671 HSNRSCHRLLPNPGDLNYPAISAVFPEKTTITSLTLH--RTVTNVGPASSSYHAIVSPFT 730
            ++    R   + G+LNYP+ S VF ++   + ++ H  RTVTNVG + S Y   + P  
Sbjct: 664 QADCDGARRAGHVGNLNYPSFSVVF-QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPR 723

Query: 731 GAAVKIEPERLNFTRRYQKLSYRITFVTKKRLSMP-----EFGGLIWKDGSHRVRSPIII 771
           G  V +EPE+L+F R  QKLS+ +   T +    P     E G ++W DG   V SP+++
Sbjct: 724 GTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVV 770

BLAST of Cp4.1LG09g01230 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 743.8 bits (1919), Expect = 1.4e-214
Identity = 390/786 (49.62%), Postives = 523/786 (66.54%), Query Frame = 0

Query: 1   MAHTPLQWLCFPLFFHCLLLSSAQFFKTYVVQMDRSAMPDSFSDHFEWYSTVLSSVVVDN 60
           MA + +  L F L F  +  +++Q  KT++ ++D  +MP  F  H+ WYST  +      
Sbjct: 1   MASSTIVLLLF-LSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAE----- 60

Query: 61  PEREGQGNGGGEDRIIYSYQNVFHGVAARLSEEEAERLEEEHGVLAVFPEVKYELHTTRS 120
                      E RI++ Y  VFHG +A ++ +EA+ L     VLAVF + + ELHTTRS
Sbjct: 61  -----------ESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRS 120

Query: 121 PKFLGLEPADSNSAWSQQIADHDVVVGVLDTGIWPESESFNDAGMSPVPAYWKGECETGR 180
           P+FLGL+  +    WS+     DV++GV DTGIWPE  SF+D  + P+P  W+G CE+G 
Sbjct: 121 PQFLGLQ--NQKGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGA 180

Query: 181 GFTKQNCNRKIVGARVFYHGYEAAT-GKFNQQLEYKSPRDQDGHGTHTAATVAGSPVAGA 240
            F+ +NCNRKI+GAR F  G +AA  G  N+ +E+ SPRD DGHGTHT++T AG     A
Sbjct: 181 RFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKA 240

Query: 241 NLLGYAYGTARGMAPGARIAAYKVCWV-GGCFSSDILSAVDRAVADGVNVLSISLGGG-- 300
           ++ GYA G A+G+AP ARIAAYKVCW   GC  SDIL+A D AV DGV+V+SIS+GGG  
Sbjct: 241 SMSGYASGVAKGVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDG 300

Query: 301 -VSSYYRDSLSVAAFGAMEMGVFVSCSAGNGGPDPVSLTNVSPWITTVGASTMDRDFPAI 360
             S YY D +++ ++GA   G+FVS SAGN GP+ +S+TN++PW+TTVGAST+DR+FPA 
Sbjct: 301 ITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPAD 360

Query: 361 VKLGNGRTITGVSLYRGRITIQENKQFPVVYMGSNSSNPDPSSLCLEGTLDPHFVAGKIV 420
             LG+G  + GVSLY G       + FPVVY G   S    +SLC+E TLDP  V GKIV
Sbjct: 361 AILGDGHRLRGVSLYAG--VPLNGRMFPVVYPG--KSGMSSASLCMENTLDPKQVRGKIV 420

Query: 421 ICDRGISPRVQKGVVVKNAGGIGMILSNTAANGEELVADCHLLPAVAVGETEGKAIKQYA 480
           ICDRG SPRV KG+VVK AGG+GMIL+N A+NGE LV D HL+PA AVG  EG  IK YA
Sbjct: 421 ICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYA 480

Query: 481 LTNRKPTATLGLLGTRLGVKPSPVVAAFSSRGPNFLTLEILKPDLVAPGVNILAAWTGKT 540
            ++  P A++   GT +G+KP+PV+A+FS RGPN L+ EILKPDL+APGVNILAAWT   
Sbjct: 481 SSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAV 540

Query: 541 GPSSLTTDTRRVKFNILSGTSMSCPHVSGVAALIKSKHPDWSPSAIKSALMTTAYVHDNT 600
           GP+ L +D R+ +FNILSGTSM+CPHVSG AAL+KS HPDWSP+ I+SA+MTT  + DN+
Sbjct: 541 GPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNS 600

Query: 601 YKPLKDASAASPSSPYDHGAGHINPRKALDPGLVYEIKPQDYFEFLCTQDLTPSQLKVFS 660
            + L D S    ++PYD+G+GH+N  +A++PGLVY+I   DY  FLC+    P  ++V +
Sbjct: 601 NRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT 660

Query: 661 KHSNRSCHRLLPNPGDLNYPAISAVFP-EKTTITSLTLHRTVTNVGPASSSYHAIVSPFT 720
           +   R      P+PG+LNYP+I+AVFP  +  + S T+ RT TNVG A + Y A +    
Sbjct: 661 RTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPR 720

Query: 721 GAAVKIEPERLNFTRRYQKLSYRITF-VTKKRLSMPE----FGGLIWKD-GSHRVRSPII 775
           G  V ++P RL FT   ++ SY +T  V  + + + E    FG + W D G H VRSPI+
Sbjct: 721 GVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIV 763