Cp4.1LG09g01130 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g01130
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein DETOXIFICATION
LocationCp4.1LG09: 640061 .. 641419 (-)
RNA-Seq ExpressionCp4.1LG09g01130
SyntenyCp4.1LG09g01130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCCTCACCAGCCTCCTTCTCTACTCCCGCTCCCTCATTTCCATGCTCTTCCTCGGCCGCCTTGGCGAGCTCTCTCTCGCCGGCGGATCCCTCGCTCTCGGCTTTGCCAACATCACCGGCTACTCCATTCTCTCTGGCCTCGCCATCGGCATGGAACCCATCTGCGGACAAGCCTTCGGCGCCAAGAAATTCACTCTCCTTGGCCTTGCTCTGCAGAGGACCATTCTTCTCCTCTCCTTGATTTCAATACCCATTTCAATTCTCTGGCTCAACGTCAAGCCTATCCTTTTGATTTGCAAACAGGACGAATCCATTGCCCACCATGCTCAATTGTTCCTTCTCTACTCTGTTCCTGACCTCTTCGCTCAATCCCTCATCCACCCACTTCGAATTTACCTCCGAACCCAATCCATAACACTCCCTCTCACTTTCTGCGCCACTTTTTCGATCTTTCTCCACATACCCATCAATTATTTTCTTGTTTCTTATCTCAATTTGGGCATTCGAGGAGTTGCAATAGCAGGGGTTTGGACAAACTTCAACTTGGTGGCTTCTTTGATTCTCTATATAGTAATCTTCCAAGTTCATAAGGAAACATGGGGAGGATTCTCGCTTGAATGCTTCAAAGAATGGGGGAAGCTTCTCAATTTGGCAATTCCAAGCTGCGTATCCGTCTGCCTCGAGTGGTGGTGGTATGAAATCATGATCTTGCTCTGTGGCCTGCTGCTAAACCCCAAAGCCACGGTGGCGTCAATGGGCATTTTGATTCAAACCACGGCATTGATTTACATATTCCCATCTTCGTTGAGCTTCAGCGTGTCGACAAGGGTGGGGAATGAATTGGGAGCAGAGCAACCAAAGAAGGCAAAATTAGCCGCCATTGTTGGGCTGTCTTGCAGCTTCGTATTGGGAATCTCTGCCCTGTTTTTCGCAGTTTCGATAAGAAAGATTTGGACGAGTATGTTCACAGAAGATAAAGAGATCATAGCACTAACGACGATGGTGCTCCCCATAATCGGACTCTGCGAGCTCGGGAACTGCCCACAGACGACGGGCTGCGGCGTATTGAGAGGAACAGCCAGGCCTAAAATCGGGGCGAATATAAATTTAGGATGTTTTTATTTGGTGGGTATGCCAGTGGCGGTGGGGCTATCATTCTACGGTGGGTTCGATTTCCGGGGACTGTGGCTGGGACTACTGGCGGCGCAGGGATGCTGTGCGGCGGCGATGTTGGTAGTTTTGGGCTTTACAGATTGGGAATTTGAAGCGATTAGAGCTAAGAAGCTAACCGGTAGCGGCGATGAAGCGGTGGAAGCTCAATCACTTATTCCTAAGAACAAGGAAGATTGCTGCTAA

mRNA sequence

ATGATCCTCACCAGCCTCCTTCTCTACTCCCGCTCCCTCATTTCCATGCTCTTCCTCGGCCGCCTTGGCGAGCTCTCTCTCGCCGGCGGATCCCTCGCTCTCGGCTTTGCCAACATCACCGGCTACTCCATTCTCTCTGGCCTCGCCATCGGCATGGAACCCATCTGCGGACAAGCCTTCGGCGCCAAGAAATTCACTCTCCTTGGCCTTGCTCTGCAGAGGACCATTCTTCTCCTCTCCTTGATTTCAATACCCATTTCAATTCTCTGGCTCAACGTCAAGCCTATCCTTTTGATTTGCAAACAGGACGAATCCATTGCCCACCATGCTCAATTGTTCCTTCTCTACTCTGTTCCTGACCTCTTCGCTCAATCCCTCATCCACCCACTTCGAATTTACCTCCGAACCCAATCCATAACACTCCCTCTCACTTTCTGCGCCACTTTTTCGATCTTTCTCCACATACCCATCAATTATTTTCTTGTTTCTTATCTCAATTTGGGCATTCGAGGAGTTGCAATAGCAGGGGTTTGGACAAACTTCAACTTGGTGGCTTCTTTGATTCTCTATATAGTAATCTTCCAAGTTCATAAGGAAACATGGGGAGGATTCTCGCTTGAATGCTTCAAAGAATGGGGGAAGCTTCTCAATTTGGCAATTCCAAGCTGCGTATCCGTCTGCCTCGAGTGGTGGTGGTATGAAATCATGATCTTGCTCTGTGGCCTGCTGCTAAACCCCAAAGCCACGGTGGCGTCAATGGGCATTTTGATTCAAACCACGGCATTGATTTACATATTCCCATCTTCGTTGAGCTTCAGCGTGTCGACAAGGGTGGGGAATGAATTGGGAGCAGAGCAACCAAAGAAGGCAAAATTAGCCGCCATTGTTGGGCTGTCTTGCAGCTTCGTATTGGGAATCTCTGCCCTGTTTTTCGCAGTTTCGATAAGAAAGATTTGGACGAGTATGTTCACAGAAGATAAAGAGATCATAGCACTAACGACGATGGTGCTCCCCATAATCGGACTCTGCGAGCTCGGGAACTGCCCACAGACGACGGGCTGCGGCGTATTGAGAGGAACAGCCAGGCCTAAAATCGGGGCGAATATAAATTTAGGATGTTTTTATTTGGTGGGTATGCCAGTGGCGGTGGGGCTATCATTCTACGGTGGGTTCGATTTCCGGGGACTGTGGCTGGGACTACTGGCGGCGCAGGGATGCTGTGCGGCGGCGATGTTGGTAGTTTTGGGCTTTACAGATTGGGAATTTGAAGCGATTAGAGCTAAGAAGCTAACCGGTAGCGGCGATGAAGCGGTGGAAGCTCAATCACTTATTCCTAAGAACAAGGAAGATTGCTGCTAA

Coding sequence (CDS)

ATGATCCTCACCAGCCTCCTTCTCTACTCCCGCTCCCTCATTTCCATGCTCTTCCTCGGCCGCCTTGGCGAGCTCTCTCTCGCCGGCGGATCCCTCGCTCTCGGCTTTGCCAACATCACCGGCTACTCCATTCTCTCTGGCCTCGCCATCGGCATGGAACCCATCTGCGGACAAGCCTTCGGCGCCAAGAAATTCACTCTCCTTGGCCTTGCTCTGCAGAGGACCATTCTTCTCCTCTCCTTGATTTCAATACCCATTTCAATTCTCTGGCTCAACGTCAAGCCTATCCTTTTGATTTGCAAACAGGACGAATCCATTGCCCACCATGCTCAATTGTTCCTTCTCTACTCTGTTCCTGACCTCTTCGCTCAATCCCTCATCCACCCACTTCGAATTTACCTCCGAACCCAATCCATAACACTCCCTCTCACTTTCTGCGCCACTTTTTCGATCTTTCTCCACATACCCATCAATTATTTTCTTGTTTCTTATCTCAATTTGGGCATTCGAGGAGTTGCAATAGCAGGGGTTTGGACAAACTTCAACTTGGTGGCTTCTTTGATTCTCTATATAGTAATCTTCCAAGTTCATAAGGAAACATGGGGAGGATTCTCGCTTGAATGCTTCAAAGAATGGGGGAAGCTTCTCAATTTGGCAATTCCAAGCTGCGTATCCGTCTGCCTCGAGTGGTGGTGGTATGAAATCATGATCTTGCTCTGTGGCCTGCTGCTAAACCCCAAAGCCACGGTGGCGTCAATGGGCATTTTGATTCAAACCACGGCATTGATTTACATATTCCCATCTTCGTTGAGCTTCAGCGTGTCGACAAGGGTGGGGAATGAATTGGGAGCAGAGCAACCAAAGAAGGCAAAATTAGCCGCCATTGTTGGGCTGTCTTGCAGCTTCGTATTGGGAATCTCTGCCCTGTTTTTCGCAGTTTCGATAAGAAAGATTTGGACGAGTATGTTCACAGAAGATAAAGAGATCATAGCACTAACGACGATGGTGCTCCCCATAATCGGACTCTGCGAGCTCGGGAACTGCCCACAGACGACGGGCTGCGGCGTATTGAGAGGAACAGCCAGGCCTAAAATCGGGGCGAATATAAATTTAGGATGTTTTTATTTGGTGGGTATGCCAGTGGCGGTGGGGCTATCATTCTACGGTGGGTTCGATTTCCGGGGACTGTGGCTGGGACTACTGGCGGCGCAGGGATGCTGTGCGGCGGCGATGTTGGTAGTTTTGGGCTTTACAGATTGGGAATTTGAAGCGATTAGAGCTAAGAAGCTAACCGGTAGCGGCGATGAAGCGGTGGAAGCTCAATCACTTATTCCTAAGAACAAGGAAGATTGCTGCTAA

Protein sequence

MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAFGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPDLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTNFNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLCGLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSCSFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGTARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDWEFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC
Homology
BLAST of Cp4.1LG09g01130 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 8.0e-175
Identity = 317/440 (72.05%), Postives = 371/440 (84.32%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELS-LAGGSLALGFANITGYSILSGLAIGMEPICGQA 60
           +ILT LLLYSRS+ISMLFLGRL +LS L+GGSLALGFANITGYS+LSGL+IGMEPIC QA
Sbjct: 43  LILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLSGLSIGMEPICVQA 102

Query: 61  FGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVP 120
           FGAK+F LLGLALQRT LLL L S+PISILWLN+K ILL   QDE I++ A++F+L+S+P
Sbjct: 103 FGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEISNQAEIFILFSLP 162

Query: 121 DLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWT 180
           DL  QS +HP+RIYLR+QSITLPLT+ A F++ LHIPINY LVS L LG++GVA+  +WT
Sbjct: 163 DLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLGLGLKGVALGAIWT 222

Query: 181 NFNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILL 240
           N NL+  LI+YIV   V+++TWGGFS++CFK W  L+ LAIPSCVSVCLEWWWYEIMILL
Sbjct: 223 NVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSVCLEWWWYEIMILL 282

Query: 241 CGLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLS 300
           CGLLLNP+ATVASMGILIQTTALIYIFPSSLS SVSTRVGNELGA QP KA++AA  GLS
Sbjct: 283 CGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQPDKARIAARTGLS 342

Query: 301 CSFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRG 360
            S  LG+ A+FFA+ +R  W  +FT+++EI+ LT+MVLPIIGLCELGNCPQTT CGVLRG
Sbjct: 343 LSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELGNCPQTTLCGVLRG 402

Query: 361 TARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTD 420
           +ARPK+GANINL CFY VGMPVAV LSF+ GFDF+GLWLGL AAQG C  +MLVVL  TD
Sbjct: 403 SARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGSCLISMLVVLARTD 462

Query: 421 WEFEAIRAKKL---TGSGDE 437
           WE E  RAK+L   +  GDE
Sbjct: 463 WEVEVHRAKELMTRSCDGDE 482

BLAST of Cp4.1LG09g01130 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.0e-150
Identity = 270/429 (62.94%), Postives = 334/429 (77.86%), Query Frame = 0

Query: 3   LTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAFGA 62
           +T LL+YSR++ISMLFLG LGEL LAGGSL++GFANITGYS++SGL++GMEPICGQA+GA
Sbjct: 71  MTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGLSMGMEPICGQAYGA 130

Query: 63  KKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPDLF 122
           K+  LLGL LQRT+LLL   S+PIS  WLN++ ILL C QDE I+  AQ FLL+++PDLF
Sbjct: 131 KQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISSVAQQFLLFAIPDLF 190

Query: 123 AQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTNFN 182
             SL+HPLRIYLRTQ+ITLP+T+    S+ LH+P+NY LV  L +G+ GVAIA V TN N
Sbjct: 191 LLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMGVAGVAIAMVLTNLN 250

Query: 183 LVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLCGL 242
           LV  L  ++    VH +TW   +++  K W  LL+LAIP+CVSVCLEWWWYE MI+LCGL
Sbjct: 251 LVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVSVCLEWWWYEFMIILCGL 310

Query: 243 LLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSCSF 302
           L NP+ATVASMGILIQTTAL+Y+FPSSLS  VSTR+ NELGA++P KA+++ I+ L C+ 
Sbjct: 311 LANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRPAKARVSMIISLFCAI 370

Query: 303 VLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGTAR 362
            LG+ A+ FAV +R  W  +FT D EI+ LT++ LPI+GLCELGNCPQTTGCGVLRG AR
Sbjct: 371 ALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGNCPQTTGCGVLRGCAR 430

Query: 363 PKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDWEF 422
           P +GANINLG FY VGMPVA+   F     F GLW GLLAAQ  CA+ ML  L  TDW+ 
Sbjct: 431 PTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATCASLMLCALLRTDWKV 490

Query: 423 EAIRAKKLT 432
           +A RA++LT
Sbjct: 491 QAERAEELT 499

BLAST of Cp4.1LG09g01130 vs. ExPASy Swiss-Prot
Match: Q9FJ87 (Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 3.9e-145
Identity = 275/460 (59.78%), Postives = 340/460 (73.91%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           ++LT L LY RS +S+ FLG LG+ +LAGGSLA  FANITGYS+ SGL +G+E IC QAF
Sbjct: 46  LVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYSLFSGLTMGVESICSQAF 105

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GA+++  +  +++R I+LL + S+P+++LW+N++ ILLI KQD+ +A  A +FLLYSVPD
Sbjct: 106 GARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQDKKLASEAHIFLLYSVPD 165

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           L AQS +HPLR+YLRTQS TLPL+ C   + FLH+PI +FLVSYL LGI+G+A++GV +N
Sbjct: 166 LVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVSYLGLGIKGIALSGVVSN 225

Query: 181 FNLVASLILYIVIFQ----------VHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEW 240
           FNLVA L LYI  F+          + +ET      +  +EW KLL LAIPSC+SVCLEW
Sbjct: 226 FNLVAFLFLYICFFEDKLSVNEDEKITEET----CEDSVREWKKLLCLAIPSCISVCLEW 285

Query: 241 WWYEIMILLCGLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKA 300
           W YEIMILLCG LL+PKA+VASMGILIQ T+L+YIFP SLS  VSTRVGNELG+ QPK+A
Sbjct: 286 WCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTRVGNELGSNQPKRA 345

Query: 301 KLAAIVGLSCSFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQ 360
           + AAIVGL  S  LG +A  F VS+R  W   FT+DKEI+ LT M LPI+GLCELGNCPQ
Sbjct: 346 RRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMALPIVGLCELGNCPQ 405

Query: 361 TTGCGVLRGTARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAA 420
           TTGCGVLRG+ARPKIGANIN   FY VG+PV   L+F+ GF F+GLWLG+LAAQ  C   
Sbjct: 406 TTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLWLGMLAAQITCVIG 465

Query: 421 MLVVLGFTDWEFEAIRAKKLTGSGDEAVEAQSLIPKNKED 451
           M+     TDWE EA RAK LT     AV+  S     KED
Sbjct: 466 MMAATCRTDWELEAERAKVLT----TAVDCGSSDDDAKED 497

BLAST of Cp4.1LG09g01130 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 6.8e-142
Identity = 256/429 (59.67%), Postives = 318/429 (74.13%), Query Frame = 0

Query: 3   LTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAFGA 62
           +T+L+LY RS +SM FLG+LG+L LA GSLA+ FANITGYS+LSGLA+GMEP+C QAFGA
Sbjct: 69  VTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSGLALGMEPLCSQAFGA 128

Query: 63  KKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPDLF 122
            +F LL L L RT++ L +  +PIS+LW NV  I +   QD  IA  AQ +L++S+PDL 
Sbjct: 129 HRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIAKLAQTYLIFSLPDLL 188

Query: 123 AQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTNFN 182
             +L+HP+RIYLR Q I  P+T  +      H+P N FLVSYL LG+ GVA+A   TN  
Sbjct: 189 TNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRLGLTGVAVASSITNIF 248

Query: 183 LVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLCGL 242
           +VA L+ Y+    +H  TW   + +CF+ W  LL LA PSCVSVCLEWWWYEIMI+LCGL
Sbjct: 249 VVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGPSCVSVCLEWWWYEIMIVLCGL 308

Query: 243 LLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSCSF 302
           L+NP++TVA+MG+LIQTT+ +Y+FPSSLSF+VSTRVGNELGA +PK AKL A V +  + 
Sbjct: 309 LVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGANRPKTAKLTATVAIVFAA 368

Query: 303 VLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGTAR 362
           V GI A  FA S+R  W  +FT DKEI+ LT   LPI+GLCE+GNCPQT GCGV+RGTAR
Sbjct: 369 VTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEIGNCPQTVGCGVVRGTAR 428

Query: 363 PKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDWEF 422
           P   AN+NLG FYLVGMPVAVGL F+ G  F GLW+GLLAAQ  CA  M+ V+G TDWE 
Sbjct: 429 PSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQISCAGLMMYVVGTTDWES 488

Query: 423 EAIRAKKLT 432
           EA +A+ LT
Sbjct: 489 EAKKAQTLT 497

BLAST of Cp4.1LG09g01130 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 8.9e-142
Identity = 259/430 (60.23%), Postives = 322/430 (74.88%), Query Frame = 0

Query: 2   ILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAFG 61
           IL +L+LY+RS ISMLFLG +GEL LAGGSLA+ FANITGYS+L+GLA+GM+P+C QAFG
Sbjct: 48  ILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFG 107

Query: 62  AKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPDL 121
           A +  LL L LQRT+L L   S+ I  LWLN+  I++   QD SI+  AQ ++L S+PDL
Sbjct: 108 AGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDL 167

Query: 122 FAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTNF 181
              S +HPLRIYLR Q IT PLT         HIP+N+FLVSYL  G  GV++A   +N 
Sbjct: 168 LTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNL 227

Query: 182 NLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLCG 241
            +V  L+ ++ I  +H+ TW   S ECFK+WG ++ LAIPSC+ VCLEWWWYEIM +LCG
Sbjct: 228 LVVIFLVAHVWIAGLHQPTWTRPSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCG 287

Query: 242 LLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSCS 301
           LL++P   VASMGILIQTT+L+YIFPSSL  +VSTRVGNELG+ +P KA+L+AIV +S +
Sbjct: 288 LLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFA 347

Query: 302 FVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGTA 361
            V+G++A  FA  +  +W  +FT D  II LT   LPI+GLCELGNCPQT GCGV+RGTA
Sbjct: 348 GVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTA 407

Query: 362 RPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDWE 421
           RP + ANINLG FYLVG PVAVGL+F+  + F GLW+GLLAAQ CCAA ML V+  TDWE
Sbjct: 408 RPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWE 467

Query: 422 FEAIRAKKLT 432
            EAIRA+KLT
Sbjct: 468 KEAIRARKLT 477

BLAST of Cp4.1LG09g01130 vs. NCBI nr
Match: XP_022944944.1 (protein DETOXIFICATION 49-like [Cucurbita moschata])

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF
Sbjct: 79  MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 138

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD
Sbjct: 139 GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 198

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN
Sbjct: 199 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 258

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC
Sbjct: 259 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 318

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 319 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 378

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 379 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 438

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 439 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 498

Query: 421 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 452
           EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC
Sbjct: 499 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 530

BLAST of Cp4.1LG09g01130 vs. NCBI nr
Match: XP_023542378.1 (protein DETOXIFICATION 49-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF
Sbjct: 79  MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 138

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD
Sbjct: 139 GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 198

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN
Sbjct: 199 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 258

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC
Sbjct: 259 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 318

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 319 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 378

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 379 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 438

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 439 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 498

Query: 421 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 452
           EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC
Sbjct: 499 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 530

BLAST of Cp4.1LG09g01130 vs. NCBI nr
Match: KAG6574153.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013207.1 Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 881 bits (2277), Expect = 0.0
Identity = 451/452 (99.78%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           MILTSLLLYSRSLISMLFLGRLGELSLAGGSLA+GFANITGYSILSGLAIGMEPICGQAF
Sbjct: 79  MILTSLLLYSRSLISMLFLGRLGELSLAGGSLAIGFANITGYSILSGLAIGMEPICGQAF 138

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD
Sbjct: 139 GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 198

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN
Sbjct: 199 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 258

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC
Sbjct: 259 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 318

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 319 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 378

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 379 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 438

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 439 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 498

Query: 421 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 452
           EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC
Sbjct: 499 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 530

BLAST of Cp4.1LG09g01130 vs. NCBI nr
Match: XP_022968021.1 (protein DETOXIFICATION 49-like [Cucurbita maxima])

HSP 1 Score: 877 bits (2267), Expect = 0.0
Identity = 449/452 (99.34%), Postives = 451/452 (99.78%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           MILTSLLLYSRSLISMLFLGRLGELSLAGGSLA+GFANITGYSILSGLAIGMEPICGQAF
Sbjct: 79  MILTSLLLYSRSLISMLFLGRLGELSLAGGSLAIGFANITGYSILSGLAIGMEPICGQAF 138

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD
Sbjct: 139 GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 198

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVS+LNLGIRGVAIAGVWTN
Sbjct: 199 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSHLNLGIRGVAIAGVWTN 258

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC
Sbjct: 259 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 318

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 319 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 378

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGISALFFAVSIRKIW SMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 379 SFVLGISALFFAVSIRKIWASMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 438

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 439 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 498

Query: 421 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 452
           EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC
Sbjct: 499 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 530

BLAST of Cp4.1LG09g01130 vs. NCBI nr
Match: XP_038880058.1 (protein DETOXIFICATION 49 [Benincasa hispida])

HSP 1 Score: 820 bits (2119), Expect = 5.13e-297
Identity = 416/454 (91.63%), Postives = 436/454 (96.04%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           M+LTSLLLYSRSLISMLFLGRLGEL LAGGSLA+GFANITGYSILSGLAIGMEPICGQAF
Sbjct: 86  MVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAIGFANITGYSILSGLAIGMEPICGQAF 145

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRTILLLSL SIPIS+LW NVK ILL+CKQD SIA  AQLFLLYSVPD
Sbjct: 146 GAKKFTLLGLALQRTILLLSLASIPISLLWFNVKNILLLCKQDHSIASQAQLFLLYSVPD 205

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLR+QSITLPLTFCA FSI LHIPINY LVSYL+ GIRGVAIAGVWTN
Sbjct: 206 LFAQSLIHPLRIYLRSQSITLPLTFCAAFSILLHIPINYLLVSYLDFGIRGVAIAGVWTN 265

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYI+IF+VHK+TWGGFSL+CFKEWG LLNLAIPSC+SVCLEWWWYEIMILLC
Sbjct: 266 FNLVASLILYILIFRVHKDTWGGFSLQCFKEWGDLLNLAIPSCISVCLEWWWYEIMILLC 325

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 326 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 385

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SF+LGI ALFFAVSIRKIW SMFT+DK+IIALT+MVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 386 SFLLGICALFFAVSIRKIWASMFTDDKDIIALTSMVLPIIGLCELGNCPQTTGCGVLRGT 445

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 446 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 505

Query: 421 EFEAIRAKKLTGSG--DEAVEAQSLIPKNKEDCC 452
           EFEAIRA+KLTG G  +E VEA+SL+PKNK+DCC
Sbjct: 506 EFEAIRARKLTGGGGEEEVVEAESLVPKNKQDCC 539

BLAST of Cp4.1LG09g01130 vs. ExPASy TrEMBL
Match: A0A6J1FZK1 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111449327 PE=3 SV=1)

HSP 1 Score: 882 bits (2279), Expect = 0.0
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF
Sbjct: 79  MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 138

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD
Sbjct: 139 GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 198

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN
Sbjct: 199 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 258

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC
Sbjct: 259 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 318

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 319 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 378

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 379 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 438

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 439 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 498

Query: 421 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 452
           EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC
Sbjct: 499 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 530

BLAST of Cp4.1LG09g01130 vs. ExPASy TrEMBL
Match: A0A6J1HTP9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111467391 PE=3 SV=1)

HSP 1 Score: 877 bits (2267), Expect = 0.0
Identity = 449/452 (99.34%), Postives = 451/452 (99.78%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           MILTSLLLYSRSLISMLFLGRLGELSLAGGSLA+GFANITGYSILSGLAIGMEPICGQAF
Sbjct: 79  MILTSLLLYSRSLISMLFLGRLGELSLAGGSLAIGFANITGYSILSGLAIGMEPICGQAF 138

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD
Sbjct: 139 GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 198

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVS+LNLGIRGVAIAGVWTN
Sbjct: 199 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSHLNLGIRGVAIAGVWTN 258

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC
Sbjct: 259 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 318

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 319 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 378

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGISALFFAVSIRKIW SMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 379 SFVLGISALFFAVSIRKIWASMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 438

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 439 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 498

Query: 421 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 452
           EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC
Sbjct: 499 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 530

BLAST of Cp4.1LG09g01130 vs. ExPASy TrEMBL
Match: A0A6J1JBI0 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482957 PE=3 SV=1)

HSP 1 Score: 820 bits (2117), Expect = 4.48e-297
Identity = 418/453 (92.27%), Postives = 433/453 (95.58%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           M+LTSLLLYSRSLISMLFLGRLGEL LAGGSLA+GFANITGYSILSGLA+GMEPICGQAF
Sbjct: 84  MVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAIGFANITGYSILSGLALGMEPICGQAF 143

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAK+F LLGLALQRTILLL+  SIPIS+LW NVK ILL+CKQD SIA  AQLFLLYSVPD
Sbjct: 144 GAKRFNLLGLALQRTILLLTFASIPISLLWFNVKNILLLCKQDASIASQAQLFLLYSVPD 203

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSI LHIPINYFLVSYLNLGIRGVAIAGVWTN
Sbjct: 204 LFAQSLIHPLRIYLRTQSITLPLTFCATFSILLHIPINYFLVSYLNLGIRGVAIAGVWTN 263

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYI+IF VHKETWGGFSLECFKEWG LLNLAIPSCVSVCLEWWWYEIMIL+C
Sbjct: 264 FNLVASLILYILIFNVHKETWGGFSLECFKEWGNLLNLAIPSCVSVCLEWWWYEIMILIC 323

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 324 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 383

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGI ALFFAVSIRKIW SMFTEDKEIIALT+MVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 384 SFVLGIMALFFAVSIRKIWASMFTEDKEIIALTSMVLPIIGLCELGNCPQTTGCGVLRGT 443

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGL+FYGGFDFRGLWLGLLAAQGCCAA MLVVLGFTDW
Sbjct: 444 ARPKIGANINLGCFYLVGMPVAVGLAFYGGFDFRGLWLGLLAAQGCCAAVMLVVLGFTDW 503

Query: 421 EFEAIRAKKLTGSG-DEAVEAQSLIPKNKEDCC 452
           EFEAIRA+KLTG G DE VEA+S+ PKNK+D C
Sbjct: 504 EFEAIRARKLTGGGGDEVVEAESVEPKNKQDWC 536

BLAST of Cp4.1LG09g01130 vs. ExPASy TrEMBL
Match: A0A5D3DG99 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00350 PE=3 SV=1)

HSP 1 Score: 817 bits (2111), Expect = 8.90e-297
Identity = 412/452 (91.15%), Postives = 434/452 (96.02%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           M+LTSLLLYSRSLISMLFLGRLGEL LAGGSLA+GFANITGYSILSGLA+GMEPICGQAF
Sbjct: 47  MVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAIGFANITGYSILSGLAMGMEPICGQAF 106

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAKKFTLLGLALQRT+LLLSL SIPIS+LW NVK ILL+CKQD SIA  AQLFLLYS+PD
Sbjct: 107 GAKKFTLLGLALQRTVLLLSLTSIPISLLWFNVKNILLLCKQDPSIASQAQLFLLYSLPD 166

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLR+QSITLPLTFCA FSI LHIPINY LVSYLN GIRGVAIAGVWTN
Sbjct: 167 LFAQSLIHPLRIYLRSQSITLPLTFCAAFSILLHIPINYLLVSYLNFGIRGVAIAGVWTN 226

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYI+IF+VHK+TW GFSL+CFKEWG LLNLAIPSC+SVCLEWWWYEIMILLC
Sbjct: 227 FNLVASLILYILIFRVHKDTWAGFSLQCFKEWGDLLNLAIPSCISVCLEWWWYEIMILLC 286

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 287 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 346

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SF+LGI ALFFAVSIRKIW SMFTEDK+IIALT+MVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 347 SFLLGICALFFAVSIRKIWASMFTEDKDIIALTSMVLPIIGLCELGNCPQTTGCGVLRGT 406

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGLSF+GGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW
Sbjct: 407 ARPKIGANINLGCFYLVGMPVAVGLSFFGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 466

Query: 421 EFEAIRAKKLTGSGDEAVEAQSLIPKNKEDCC 452
           EFEAIRA+KLTG  +E VEA+SL+PKNK+DCC
Sbjct: 467 EFEAIRARKLTGGCEEVVEAESLMPKNKQDCC 498

BLAST of Cp4.1LG09g01130 vs. ExPASy TrEMBL
Match: A0A6J1E4L7 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430734 PE=3 SV=1)

HSP 1 Score: 817 bits (2111), Expect = 2.35e-296
Identity = 418/453 (92.27%), Postives = 432/453 (95.36%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           M+LTSLLLYSRSLISMLFLGRLGEL LAGGSLA+GFANITGYSILSGLA+GMEPICGQAF
Sbjct: 72  MVLTSLLLYSRSLISMLFLGRLGELPLAGGSLAIGFANITGYSILSGLAMGMEPICGQAF 131

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GAK+F LLGLALQRTILLL+  SIPIS+LW NVK ILL+CKQD SIA  AQLFLLYSVPD
Sbjct: 132 GAKRFNLLGLALQRTILLLTFASIPISLLWFNVKNILLLCKQDASIASQAQLFLLYSVPD 191

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           LFAQSLIHPLRIYLRTQSITLPLTFCATFSI LHIPINYFLVSYLN GIRGVAIAGVWTN
Sbjct: 192 LFAQSLIHPLRIYLRTQSITLPLTFCATFSILLHIPINYFLVSYLNFGIRGVAIAGVWTN 251

Query: 181 FNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLC 240
           FNLVASLILYI+IF VHKETWGGFSLECFKEWG LLNLAIPSCVSVCLEWWWYEIMILLC
Sbjct: 252 FNLVASLILYILIFNVHKETWGGFSLECFKEWGNLLNLAIPSCVSVCLEWWWYEIMILLC 311

Query: 241 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 300
           GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC
Sbjct: 312 GLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSC 371

Query: 301 SFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGT 360
           SFVLGI ALFFAVSIRKIW SMFTEDKEIIALT+MVLPIIGLCELGNCPQTTGCGVLRGT
Sbjct: 372 SFVLGIMALFFAVSIRKIWASMFTEDKEIIALTSMVLPIIGLCELGNCPQTTGCGVLRGT 431

Query: 361 ARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDW 420
           ARPKIGANINLGCFYLVGMPVAVGL+FYGGFDFRGLWLGLLAAQGCCAA MLVVLGFTDW
Sbjct: 432 ARPKIGANINLGCFYLVGMPVAVGLAFYGGFDFRGLWLGLLAAQGCCAAVMLVVLGFTDW 491

Query: 421 EFEAIRAKKLTGSG-DEAVEAQSLIPKNKEDCC 452
           E EAIRA+KLTG G DEAVEA+S+ PKNK+D C
Sbjct: 492 ELEAIRARKLTGGGGDEAVEAESVEPKNKQDRC 524

BLAST of Cp4.1LG09g01130 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 614.8 bits (1584), Expect = 5.7e-176
Identity = 317/440 (72.05%), Postives = 371/440 (84.32%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELS-LAGGSLALGFANITGYSILSGLAIGMEPICGQA 60
           +ILT LLLYSRS+ISMLFLGRL +LS L+GGSLALGFANITGYS+LSGL+IGMEPIC QA
Sbjct: 43  LILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLSGLSIGMEPICVQA 102

Query: 61  FGAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVP 120
           FGAK+F LLGLALQRT LLL L S+PISILWLN+K ILL   QDE I++ A++F+L+S+P
Sbjct: 103 FGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEISNQAEIFILFSLP 162

Query: 121 DLFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWT 180
           DL  QS +HP+RIYLR+QSITLPLT+ A F++ LHIPINY LVS L LG++GVA+  +WT
Sbjct: 163 DLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLGLGLKGVALGAIWT 222

Query: 181 NFNLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILL 240
           N NL+  LI+YIV   V+++TWGGFS++CFK W  L+ LAIPSCVSVCLEWWWYEIMILL
Sbjct: 223 NVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSVCLEWWWYEIMILL 282

Query: 241 CGLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLS 300
           CGLLLNP+ATVASMGILIQTTALIYIFPSSLS SVSTRVGNELGA QP KA++AA  GLS
Sbjct: 283 CGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQPDKARIAARTGLS 342

Query: 301 CSFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRG 360
            S  LG+ A+FFA+ +R  W  +FT+++EI+ LT+MVLPIIGLCELGNCPQTT CGVLRG
Sbjct: 343 LSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELGNCPQTTLCGVLRG 402

Query: 361 TARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTD 420
           +ARPK+GANINL CFY VGMPVAV LSF+ GFDF+GLWLGL AAQG C  +MLVVL  TD
Sbjct: 403 SARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGSCLISMLVVLARTD 462

Query: 421 WEFEAIRAKKL---TGSGDE 437
           WE E  RAK+L   +  GDE
Sbjct: 463 WEVEVHRAKELMTRSCDGDE 482

BLAST of Cp4.1LG09g01130 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 7.5e-152
Identity = 270/429 (62.94%), Postives = 334/429 (77.86%), Query Frame = 0

Query: 3   LTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAFGA 62
           +T LL+YSR++ISMLFLG LGEL LAGGSL++GFANITGYS++SGL++GMEPICGQA+GA
Sbjct: 71  MTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGLSMGMEPICGQAYGA 130

Query: 63  KKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPDLF 122
           K+  LLGL LQRT+LLL   S+PIS  WLN++ ILL C QDE I+  AQ FLL+++PDLF
Sbjct: 131 KQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISSVAQQFLLFAIPDLF 190

Query: 123 AQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTNFN 182
             SL+HPLRIYLRTQ+ITLP+T+    S+ LH+P+NY LV  L +G+ GVAIA V TN N
Sbjct: 191 LLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMGVAGVAIAMVLTNLN 250

Query: 183 LVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLCGL 242
           LV  L  ++    VH +TW   +++  K W  LL+LAIP+CVSVCLEWWWYE MI+LCGL
Sbjct: 251 LVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVSVCLEWWWYEFMIILCGL 310

Query: 243 LLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSCSF 302
           L NP+ATVASMGILIQTTAL+Y+FPSSLS  VSTR+ NELGA++P KA+++ I+ L C+ 
Sbjct: 311 LANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRPAKARVSMIISLFCAI 370

Query: 303 VLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGTAR 362
            LG+ A+ FAV +R  W  +FT D EI+ LT++ LPI+GLCELGNCPQTTGCGVLRG AR
Sbjct: 371 ALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGNCPQTTGCGVLRGCAR 430

Query: 363 PKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDWEF 422
           P +GANINLG FY VGMPVA+   F     F GLW GLLAAQ  CA+ ML  L  TDW+ 
Sbjct: 431 PTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATCASLMLCALLRTDWKV 490

Query: 423 EAIRAKKLT 432
           +A RA++LT
Sbjct: 491 QAERAEELT 499

BLAST of Cp4.1LG09g01130 vs. TAIR 10
Match: AT5G52050.1 (MATE efflux family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 2.7e-146
Identity = 275/460 (59.78%), Postives = 340/460 (73.91%), Query Frame = 0

Query: 1   MILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAF 60
           ++LT L LY RS +S+ FLG LG+ +LAGGSLA  FANITGYS+ SGL +G+E IC QAF
Sbjct: 46  LVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYSLFSGLTMGVESICSQAF 105

Query: 61  GAKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPD 120
           GA+++  +  +++R I+LL + S+P+++LW+N++ ILLI KQD+ +A  A +FLLYSVPD
Sbjct: 106 GARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQDKKLASEAHIFLLYSVPD 165

Query: 121 LFAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTN 180
           L AQS +HPLR+YLRTQS TLPL+ C   + FLH+PI +FLVSYL LGI+G+A++GV +N
Sbjct: 166 LVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVSYLGLGIKGIALSGVVSN 225

Query: 181 FNLVASLILYIVIFQ----------VHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEW 240
           FNLVA L LYI  F+          + +ET      +  +EW KLL LAIPSC+SVCLEW
Sbjct: 226 FNLVAFLFLYICFFEDKLSVNEDEKITEET----CEDSVREWKKLLCLAIPSCISVCLEW 285

Query: 241 WWYEIMILLCGLLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKA 300
           W YEIMILLCG LL+PKA+VASMGILIQ T+L+YIFP SLS  VSTRVGNELG+ QPK+A
Sbjct: 286 WCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTRVGNELGSNQPKRA 345

Query: 301 KLAAIVGLSCSFVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQ 360
           + AAIVGL  S  LG +A  F VS+R  W   FT+DKEI+ LT M LPI+GLCELGNCPQ
Sbjct: 346 RRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMALPIVGLCELGNCPQ 405

Query: 361 TTGCGVLRGTARPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAA 420
           TTGCGVLRG+ARPKIGANIN   FY VG+PV   L+F+ GF F+GLWLG+LAAQ  C   
Sbjct: 406 TTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLWLGMLAAQITCVIG 465

Query: 421 MLVVLGFTDWEFEAIRAKKLTGSGDEAVEAQSLIPKNKED 451
           M+     TDWE EA RAK LT     AV+  S     KED
Sbjct: 466 MMAATCRTDWELEAERAKVLT----TAVDCGSSDDDAKED 497

BLAST of Cp4.1LG09g01130 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 4.8e-143
Identity = 256/429 (59.67%), Postives = 318/429 (74.13%), Query Frame = 0

Query: 3   LTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAFGA 62
           +T+L+LY RS +SM FLG+LG+L LA GSLA+ FANITGYS+LSGLA+GMEP+C QAFGA
Sbjct: 69  VTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSGLALGMEPLCSQAFGA 128

Query: 63  KKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPDLF 122
            +F LL L L RT++ L +  +PIS+LW NV  I +   QD  IA  AQ +L++S+PDL 
Sbjct: 129 HRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIAKLAQTYLIFSLPDLL 188

Query: 123 AQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTNFN 182
             +L+HP+RIYLR Q I  P+T  +      H+P N FLVSYL LG+ GVA+A   TN  
Sbjct: 189 TNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRLGLTGVAVASSITNIF 248

Query: 183 LVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLCGL 242
           +VA L+ Y+    +H  TW   + +CF+ W  LL LA PSCVSVCLEWWWYEIMI+LCGL
Sbjct: 249 VVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGPSCVSVCLEWWWYEIMIVLCGL 308

Query: 243 LLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSCSF 302
           L+NP++TVA+MG+LIQTT+ +Y+FPSSLSF+VSTRVGNELGA +PK AKL A V +  + 
Sbjct: 309 LVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGANRPKTAKLTATVAIVFAA 368

Query: 303 VLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGTAR 362
           V GI A  FA S+R  W  +FT DKEI+ LT   LPI+GLCE+GNCPQT GCGV+RGTAR
Sbjct: 369 VTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEIGNCPQTVGCGVVRGTAR 428

Query: 363 PKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDWEF 422
           P   AN+NLG FYLVGMPVAVGL F+ G  F GLW+GLLAAQ  CA  M+ V+G TDWE 
Sbjct: 429 PSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQISCAGLMMYVVGTTDWES 488

Query: 423 EAIRAKKLT 432
           EA +A+ LT
Sbjct: 489 EAKKAQTLT 497

BLAST of Cp4.1LG09g01130 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 505.0 bits (1299), Expect = 6.3e-143
Identity = 259/430 (60.23%), Postives = 322/430 (74.88%), Query Frame = 0

Query: 2   ILTSLLLYSRSLISMLFLGRLGELSLAGGSLALGFANITGYSILSGLAIGMEPICGQAFG 61
           IL +L+LY+RS ISMLFLG +GEL LAGGSLA+ FANITGYS+L+GLA+GM+P+C QAFG
Sbjct: 48  ILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFG 107

Query: 62  AKKFTLLGLALQRTILLLSLISIPISILWLNVKPILLICKQDESIAHHAQLFLLYSVPDL 121
           A +  LL L LQRT+L L   S+ I  LWLN+  I++   QD SI+  AQ ++L S+PDL
Sbjct: 108 AGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDL 167

Query: 122 FAQSLIHPLRIYLRTQSITLPLTFCATFSIFLHIPINYFLVSYLNLGIRGVAIAGVWTNF 181
              S +HPLRIYLR Q IT PLT         HIP+N+FLVSYL  G  GV++A   +N 
Sbjct: 168 LTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNL 227

Query: 182 NLVASLILYIVIFQVHKETWGGFSLECFKEWGKLLNLAIPSCVSVCLEWWWYEIMILLCG 241
            +V  L+ ++ I  +H+ TW   S ECFK+WG ++ LAIPSC+ VCLEWWWYEIM +LCG
Sbjct: 228 LVVIFLVAHVWIAGLHQPTWTRPSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCG 287

Query: 242 LLLNPKATVASMGILIQTTALIYIFPSSLSFSVSTRVGNELGAEQPKKAKLAAIVGLSCS 301
           LL++P   VASMGILIQTT+L+YIFPSSL  +VSTRVGNELG+ +P KA+L+AIV +S +
Sbjct: 288 LLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFA 347

Query: 302 FVLGISALFFAVSIRKIWTSMFTEDKEIIALTTMVLPIIGLCELGNCPQTTGCGVLRGTA 361
            V+G++A  FA  +  +W  +FT D  II LT   LPI+GLCELGNCPQT GCGV+RGTA
Sbjct: 348 GVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTA 407

Query: 362 RPKIGANINLGCFYLVGMPVAVGLSFYGGFDFRGLWLGLLAAQGCCAAAMLVVLGFTDWE 421
           RP + ANINLG FYLVG PVAVGL+F+  + F GLW+GLLAAQ CCAA ML V+  TDWE
Sbjct: 408 RPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWE 467

Query: 422 FEAIRAKKLT 432
            EAIRA+KLT
Sbjct: 468 KEAIRARKLT 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O827528.0e-17572.05Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1[more]
Q9SLV01.0e-15062.94Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1[more]
Q9FJ873.9e-14559.78Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1[more]
Q9SZE26.8e-14259.67Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1[more]
Q4PSF48.9e-14260.23Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022944944.10.0100.00protein DETOXIFICATION 49-like [Cucurbita moschata][more]
XP_023542378.10.0100.00protein DETOXIFICATION 49-like [Cucurbita pepo subsp. pepo][more]
KAG6574153.10.099.78Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_022968021.10.099.34protein DETOXIFICATION 49-like [Cucurbita maxima][more]
XP_038880058.15.13e-29791.63protein DETOXIFICATION 49 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FZK10.0100.00Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111449327 PE=3 SV=1[more]
A0A6J1HTP90.099.34Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111467391 PE=3 SV=1[more]
A0A6J1JBI04.48e-29792.27Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111482957 PE=3 SV=1[more]
A0A5D3DG998.90e-29791.15Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... [more]
A0A6J1E4L72.35e-29692.27Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430734 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G23030.15.7e-17672.05MATE efflux family protein [more]
AT1G58340.17.5e-15262.94MATE efflux family protein [more]
AT5G52050.12.7e-14659.78MATE efflux family protein [more]
AT4G29140.14.8e-14359.67MATE efflux family protein [more]
AT5G19700.16.3e-14360.23MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 1..397
e-value: 2.1E-67
score: 225.7
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 221..382
e-value: 4.9E-36
score: 123.8
coord: 1..160
e-value: 3.9E-36
score: 124.1
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 1..438
NoneNo IPR availablePANTHERPTHR11206:SF394PROTEIN DETOXIFICATIONcoord: 1..438
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 1..424
e-value: 3.35569E-168
score: 478.221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g01130.1Cp4.1LG09g01130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity