Cp4.1LG08g12420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG08g12420 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 9.7e-120
Identity = 202/341 (59.24%), Postives = 256/341 (75.07%), Query Frame = 0

Query: 7   WPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE-VT 66
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     + +    IPV+DM  +    E + 
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLR 64

Query: 67  AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRLG 126
            +  AC+EWGFF ++NHG+   +ME ++  W +FF  PL+ KR+YANSP TYEGYGSRLG
Sbjct: 65  LVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLG 124

Query: 127 IEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSMG 186
           + K A LDWSDYFF+NYLP S+RN +KWP+ PP  +ELIE+YG+E+ KLCE++ + LS  
Sbjct: 125 VVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSES 184

Query: 187 LGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 246
           LGL+   L++A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V GL
Sbjct: 185 LGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGL 244

Query: 247 QVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPRS 306
           QVR+GD W+TI  +PNALIVNI DQ+Q+LSNGIYKSV H+VIVNS  +RVSLAFFYNPRS
Sbjct: 245 QVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRS 304

Query: 307 DLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQ 346
           D+ + P + LVT  RPAL+ P  FDEYR  IR++G CG +Q
Sbjct: 305 DIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQ 345

BLAST of Cp4.1LG08g12420 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.8e-118
Identity = 202/352 (57.39%), Postives = 263/352 (74.72%), Query Frame = 0

Query: 5   ESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERN---VPRNIPVVDMEKLQSA 64
           E WPEP+V VQSLAES + ++P RY+KPPSQRP    ++        NIP++D++ L S 
Sbjct: 47  EKWPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSG 106

Query: 65  AE--VTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEG 124
            E     ++EAC+EWGFF VINHG+  E+M+  ++ W  FF+ P++ K  Y+NSP TYEG
Sbjct: 107 NEDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEG 166

Query: 125 YGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVM 184
           YGSRLG+EK AILDW+DY+++++LP++L++  KWP+ P + +E+ +EYGKE+VKL  ++M
Sbjct: 167 YGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLM 226

Query: 185 KGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 244
             LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD
Sbjct: 227 TILSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPD 286

Query: 245 HNVPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAF 304
             V GLQVR GD WIT++P+ +A IVNI DQIQ+LSN  YKSV HRVIVNS K+RVSLAF
Sbjct: 287 DQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAF 346

Query: 305 FYNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 351
           FYNP+SD+ I+P + LVT   P L+PP TFD+YRL IR +G  G S  E  I
Sbjct: 347 FYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKSHVESHI 397

BLAST of Cp4.1LG08g12420 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.6e-117
Identity = 200/354 (56.50%), Postives = 263/354 (74.29%), Query Frame = 0

Query: 4   LESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE 63
           ++ WPEP+V VQSLAES + ++P RY+KP S RP+    +     NIP++D+E L S   
Sbjct: 17  MDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPT-TTEDAPTATNIPIIDLEGLFSEEG 76

Query: 64  VT------AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTY 123
           ++       ++EAC+ WGFF V+NHG+  E+M+  ++ W +FFH P++ K  Y+NSP TY
Sbjct: 77  LSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTY 136

Query: 124 EGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEK 183
           EGYGSRLG+EK A LDWSDY+F++ LP  L++  KWP+FPP+ +E+I+EYG+E+VKL  +
Sbjct: 137 EGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGR 196

Query: 184 VMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILL 243
           +M+ LS  LGL+E+   +AFGG E IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILL
Sbjct: 197 IMRVLSTNLGLKEDKFQEAFGG-ENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 256

Query: 244 PDHNVPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSL 303
           PD  V GLQVRK D WIT+ P P+A IVNI DQIQ+LSN  YKSV HRVIVNS+K+RVSL
Sbjct: 257 PDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSL 316

Query: 304 AFFYNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 351
           AFFYNP+SD+ I+P + LV+   P L+PP TFD+YRL IR +G  G S  E  I
Sbjct: 317 AFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKSHVESHI 368

BLAST of Cp4.1LG08g12420 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 3.3e-112
Identity = 194/349 (55.59%), Postives = 258/349 (73.93%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPR--NIPVVDMEKL 60
           M+  + WPEP+V VQSL+ES +  IP RYVKP SQRP+     ++ P+   IP++D+ +L
Sbjct: 1   MNIFQDWPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRL 60

Query: 61  QS------AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYAN 120
            +      A  +  +++AC+E GFF V+NHG++ ++M+  K  W +FF+ P++LK  +AN
Sbjct: 61  YTDDLTLQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHAN 120

Query: 121 SPTTYEGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFP-PSKELIEEYGKEMV 180
           SP TYEGYGSRLG+EK AILDWSDY++++Y P SL++  KWP+ P   +E++E+Y KEMV
Sbjct: 121 SPKTYEGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMV 180

Query: 181 KLCEKVMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 240
           KLCE +MK LS  LGL+E+ L  AFGG+E  G C+R N YPKCPQP+LTLG+SPHSDPGG
Sbjct: 181 KLCENLMKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGG 240

Query: 241 ITILLPDHNVPGLQVRKGDD-WITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSN 300
           +TILLPD  V  LQVR  DD WIT++P P+A IVN+ DQIQ+LSN IYKSV HRVIVN  
Sbjct: 241 LTILLPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPE 300

Query: 301 KDRVSLAFFYNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRG 340
            +R+SLAFFYNP+ ++ IEP K LVT + PAL+  TT+D YR  IR +G
Sbjct: 301 NERLSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQG 349

BLAST of Cp4.1LG08g12420 vs. ExPASy Swiss-Prot
Match: P51091 (Leucoanthocyanidin dioxygenase OS=Malus domestica OX=3750 GN=ANS PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.4e-62
Identity = 127/333 (38.14%), Postives = 197/333 (59.16%), Query Frame = 0

Query: 14  VQSLAESGIRTIPVRYVKPPSQRPSGAAV----ERNVPRNIPVVDMEKLQSAAEVT---- 73
           V++LA SGI TIP  Y++P  +  +   +    + N    +P +D+++++S  E      
Sbjct: 11  VETLAGSGISTIPKEYIRPKDELVNIGDIFEQEKNNEGPQVPTIDLKEIESDNEKVRAKC 70

Query: 74  --AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTT--YEGYG 133
              + +A  +WG  H++NHGI+ E+M+ ++     FF  P++ K +YAN   +   +GYG
Sbjct: 71  REKLKKAAVDWGVMHLVNHGISDELMDKVRKAGKAFFDLPIEQKEKYANDQASGKIQGYG 130

Query: 134 SRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSK-ELIEEYGKEMVKLCEKVMKG 193
           S+L    +  L+W DYFF    P   R+ + WP  P    E   EY K++ +L  KV+K 
Sbjct: 131 SKLANNASGQLEWEDYFFHCVYPEDKRDLSIWPQTPADYIEATAEYAKQLRELATKVLKV 190

Query: 194 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 253
           LS+GLGL+E  L K  GG E +   M+ N YPKCPQP+L LG+  H+D   +T +L  HN
Sbjct: 191 LSLGLGLDEGRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAHTDVSALTFIL--HN 250

Query: 254 -VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFF 313
            VPGLQ+     W+T   +PN+++++I D +++LSNG YKS+ HR +VN  K R+S A F
Sbjct: 251 MVPGLQLFYEGKWVTAKCVPNSIVMHIGDTLEILSNGKYKSILHRGMVNKEKVRISWAVF 310

Query: 314 YN-PRSDLIIEPAKVLVTEERPALFPPTTFDEY 332
              P+  +I++P    V+E+ PA+FPP TF E+
Sbjct: 311 CEPPKEKIILKPLPETVSEDEPAMFPPRTFAEH 341

BLAST of Cp4.1LG08g12420 vs. NCBI nr
Match: XP_023539287.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita pepo subsp. pepo])

HSP 1 Score: 718 bits (1853), Expect = 2.39e-261
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS
Sbjct: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350

BLAST of Cp4.1LG08g12420 vs. NCBI nr
Match: XP_022948721.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita moschata])

HSP 1 Score: 709 bits (1830), Expect = 7.68e-258
Identity = 345/350 (98.57%), Postives = 347/350 (99.14%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEV AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVN+ADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           NPRSDLIIEPAKVLVTEERPALFPP TFDEYRLQIR+RGICGTSQQEQSI
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 350

BLAST of Cp4.1LG08g12420 vs. NCBI nr
Match: KAG6596343.1 (putative 2-oxoglutarate-dependent dioxygenase ANS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 708 bits (1828), Expect = 1.55e-257
Identity = 345/349 (98.85%), Postives = 346/349 (99.14%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEV AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVN+ADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQS 349
           NPRSDLIIEPAKVLVTEERPALFPP TFDEYRLQIRKRGICGTSQQEQS
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRKRGICGTSQQEQS 349

BLAST of Cp4.1LG08g12420 vs. NCBI nr
Match: XP_022971580.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita maxima])

HSP 1 Score: 703 bits (1814), Expect = 2.11e-255
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAV+RNV RNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVKRNVWRNIPVVDMEKLQS 60

Query: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEK AILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKGAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVN+ADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           NPRSDLIIEPAKVLVTEERPALFPP TFDEYRLQIRKRGICGT QQEQSI
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRKRGICGTLQQEQSI 350

BLAST of Cp4.1LG08g12420 vs. NCBI nr
Match: XP_023528714.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 606 bits (1563), Expect = 3.91e-217
Identity = 297/347 (85.59%), Postives = 314/347 (90.49%), Query Frame = 0

Query: 6   SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVER-NVPRNIPVVDMEKLQSAAEV 65
           SWPEPVVCVQSLAESGIRTIPVRYVKPP QRP GAA+E   V RNIPVVDM KL+SAA V
Sbjct: 2   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAV 61

Query: 66  TAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRL 125
             MAEAC+EWGFFHVINHGI+GE+MEC+K+ W QFF QPLDLK+QYANSP TYEGYGSRL
Sbjct: 62  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSM 185
           GIEK AILDWSDYFF+NY+P SLRN A WPAFPPS K+LIEEYG E VKLC K+MK LS+
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGNEAVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPR 305
           LQVRKGD WITIDPIPNALIVNI DQIQVLSN IYKSV HRVIVNSNKDRVSLAFFYNP+
Sbjct: 242 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           SDLIIEPAK LV+EERPALFP  TFDEYRL IRKRGICGTSQ EQS+
Sbjct: 302 SDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSL 348

BLAST of Cp4.1LG08g12420 vs. ExPASy TrEMBL
Match: A0A6J1G9Z8 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita moschata OX=3662 GN=LOC111452304 PE=3 SV=1)

HSP 1 Score: 709 bits (1830), Expect = 3.72e-258
Identity = 345/350 (98.57%), Postives = 347/350 (99.14%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEV AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVN+ADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           NPRSDLIIEPAKVLVTEERPALFPP TFDEYRLQIR+RGICGTSQQEQSI
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 350

BLAST of Cp4.1LG08g12420 vs. ExPASy TrEMBL
Match: A0A6J1I649 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN=LOC111470253 PE=3 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 1.02e-255
Identity = 343/350 (98.00%), Postives = 345/350 (98.57%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCL SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAV+RNV RNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVKRNVWRNIPVVDMEKLQS 60

Query: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEK AILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKGAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVN+ADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           NPRSDLIIEPAKVLVTEERPALFPP TFDEYRLQIRKRGICGT QQEQSI
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRKRGICGTLQQEQSI 350

BLAST of Cp4.1LG08g12420 vs. ExPASy TrEMBL
Match: A0A6J1J756 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3661 GN=LOC111481976 PE=3 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 3.82e-217
Identity = 295/347 (85.01%), Postives = 316/347 (91.07%), Query Frame = 0

Query: 6   SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVER-NVPRNIPVVDMEKLQSAAEV 65
           SWPEPVVCVQSLAESGI+TIPVRYVKPP QRP GAA+E   V RNIP+VDM KL+SAA V
Sbjct: 2   SWPEPVVCVQSLAESGIKTIPVRYVKPPCQRPGGAAMESVEVQRNIPMVDMAKLESAAAV 61

Query: 66  TAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRL 125
             MAEAC+EWGFFHVINHGI+GE+MEC+K+ W QFF QPLDLK+QYANSP TYEGYGSRL
Sbjct: 62  RDMAEACQEWGFFHVINHGISGELMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSM 185
           GIEK AILDWSDYFF+NY+P SLRN A WPAFPPS K+LIEEYG E+VKLC K+MK LS+
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGSEVVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPR 305
           LQVRKGD+WITIDPIPNALIVNI DQIQVLSN IYKSV HRVIVNSNKDRVSLAFFYNP+
Sbjct: 242 LQVRKGDNWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           SDLIIEPAK LVTEERPALFP  TFDEYRL IRKRGICGTSQ EQS+
Sbjct: 302 SDLIIEPAKELVTEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSL 348

BLAST of Cp4.1LG08g12420 vs. ExPASy TrEMBL
Match: A0A6J1F503 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111442217 PE=3 SV=1)

HSP 1 Score: 603 bits (1555), Expect = 3.14e-216
Identity = 295/347 (85.01%), Postives = 314/347 (90.49%), Query Frame = 0

Query: 6   SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVER-NVPRNIPVVDMEKLQSAAEV 65
           SWPEPVVCVQSLAESGIRTIPVRYVKPP QRP GAA+E   V  NIPVVDM KL+SAA V
Sbjct: 2   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQMNIPVVDMAKLESAAAV 61

Query: 66  TAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRL 125
             MAEAC+EWGFFHVINHGI+GE+MEC+K+ W QFF QPLDLK+QYANSP TYEGYGSRL
Sbjct: 62  RDMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSM 185
           GIEK AILDWSDYFF+NY+P SLRN A WPAFPPS K+LIEEYG E+VKLC K+MK LS+
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSTKKLIEEYGNEVVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPR 305
           LQVRKGD WITIDPIPNALIVNI DQIQVLSN IYKSV HRVIVNSNKDRVSLAFFYNP+
Sbjct: 242 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 350
           SDLIIEPAK LV+EERPALFP  TFDEYRL IRKRGICGTSQ EQS+
Sbjct: 302 SDLIIEPAKELVSEERPALFPRMTFDEYRLDIRKRGICGTSQHEQSL 348

BLAST of Cp4.1LG08g12420 vs. ExPASy TrEMBL
Match: A0A0A0LCS8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731160 PE=3 SV=1)

HSP 1 Score: 574 bits (1479), Expect = 1.10e-204
Identity = 274/348 (78.74%), Postives = 309/348 (88.79%), Query Frame = 0

Query: 2   SCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSA 61
           SCL+SWPEPVVCVQSLAESGI  IP RYVKPPSQRP G  V++   RNIPVVDMEK++S 
Sbjct: 4   SCLQSWPEPVVCVQSLAESGISKIPGRYVKPPSQRPDGGTVKKM--RNIPVVDMEKVESG 63

Query: 62  AEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYG 121
           A V  MAEAC+EWGFF +INHGI+GEM+EC+K+ W +FF+QPLDLK QYAN+P TYEGYG
Sbjct: 64  AAVKEMAEACREWGFFQIINHGISGEMIECVKESWKEFFNQPLDLKNQYANTPATYEGYG 123

Query: 122 SRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKG 181
           SRLGIEK AILDWSDYFF+N+LP+SLRN  KWPAFP S K++IEEYG E+VK+C K+MKG
Sbjct: 124 SRLGIEKGAILDWSDYFFLNFLPLSLRNPTKWPAFPSSFKKVIEEYGDEVVKVCAKLMKG 183

Query: 182 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 241
           LSMGLGLEEEYLL AFGGE+GIG  MRANMYPKCPQPDLTLGLS HSDPGGITILL DHN
Sbjct: 184 LSMGLGLEEEYLLNAFGGEKGIGGSMRANMYPKCPQPDLTLGLSSHSDPGGITILLADHN 243

Query: 242 VPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 301
           VPGLQV KG+DWIT+DPIPNALIVNI DQIQVLSNGIYKSV HRV+VN NK+RVSLAFFY
Sbjct: 244 VPGLQVLKGNDWITVDPIPNALIVNIGDQIQVLSNGIYKSVKHRVMVNPNKERVSLAFFY 303

Query: 302 NPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQ 348
           NP+ DLIIEP+K L+T+ERP+LFPP TFDEYRL IRK+GICGTS+ +Q
Sbjct: 304 NPKGDLIIEPSKELLTKERPSLFPPMTFDEYRLYIRKKGICGTSKHQQ 349

BLAST of Cp4.1LG08g12420 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 431.4 bits (1108), Expect = 6.9e-121
Identity = 202/341 (59.24%), Postives = 256/341 (75.07%), Query Frame = 0

Query: 7   WPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE-VT 66
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     + +    IPV+DM  +    E + 
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLR 64

Query: 67  AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRLG 126
            +  AC+EWGFF ++NHG+   +ME ++  W +FF  PL+ KR+YANSP TYEGYGSRLG
Sbjct: 65  LVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLG 124

Query: 127 IEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSMG 186
           + K A LDWSDYFF+NYLP S+RN +KWP+ PP  +ELIE+YG+E+ KLCE++ + LS  
Sbjct: 125 VVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSES 184

Query: 187 LGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 246
           LGL+   L++A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V GL
Sbjct: 185 LGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGL 244

Query: 247 QVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPRS 306
           QVR+GD W+TI  +PNALIVNI DQ+Q+LSNGIYKSV H+VIVNS  +RVSLAFFYNPRS
Sbjct: 245 QVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRS 304

Query: 307 DLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQ 346
           D+ + P + LVT  RPAL+ P  FDEYR  IR++G CG +Q
Sbjct: 305 DIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQ 345

BLAST of Cp4.1LG08g12420 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 427.2 bits (1097), Expect = 1.3e-119
Identity = 202/352 (57.39%), Postives = 263/352 (74.72%), Query Frame = 0

Query: 5   ESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERN---VPRNIPVVDMEKLQSA 64
           E WPEP+V VQSLAES + ++P RY+KPPSQRP    ++        NIP++D++ L S 
Sbjct: 47  EKWPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSG 106

Query: 65  AE--VTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEG 124
            E     ++EAC+EWGFF VINHG+  E+M+  ++ W  FF+ P++ K  Y+NSP TYEG
Sbjct: 107 NEDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEG 166

Query: 125 YGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVM 184
           YGSRLG+EK AILDW+DY+++++LP++L++  KWP+ P + +E+ +EYGKE+VKL  ++M
Sbjct: 167 YGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLM 226

Query: 185 KGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 244
             LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD
Sbjct: 227 TILSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPD 286

Query: 245 HNVPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAF 304
             V GLQVR GD WIT++P+ +A IVNI DQIQ+LSN  YKSV HRVIVNS K+RVSLAF
Sbjct: 287 DQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAF 346

Query: 305 FYNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 351
           FYNP+SD+ I+P + LVT   P L+PP TFD+YRL IR +G  G S  E  I
Sbjct: 347 FYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKSHVESHI 397

BLAST of Cp4.1LG08g12420 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 423.7 bits (1088), Expect = 1.4e-118
Identity = 198/341 (58.06%), Postives = 259/341 (75.95%), Query Frame = 0

Query: 5   ESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERN---VPRNIPVVDMEKLQSA 64
           E WPEP+V VQSLAES + ++P RY+KPPSQRP    ++        NIP++D++ L S 
Sbjct: 47  EKWPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSG 106

Query: 65  AE--VTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEG 124
            E     ++EAC+EWGFF VINHG+  E+M+  ++ W  FF+ P++ K  Y+NSP TYEG
Sbjct: 107 NEDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEG 166

Query: 125 YGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVM 184
           YGSRLG+EK AILDW+DY+++++LP++L++  KWP+ P + +E+ +EYGKE+VKL  ++M
Sbjct: 167 YGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLM 226

Query: 185 KGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 244
             LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD
Sbjct: 227 TILSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPD 286

Query: 245 HNVPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAF 304
             V GLQVR GD WIT++P+ +A IVNI DQIQ+LSN  YKSV HRVIVNS K+RVSLAF
Sbjct: 287 DQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAF 346

Query: 305 FYNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRG 340
           FYNP+SD+ I+P + LVT   P L+PP TFD+YRL IR +G
Sbjct: 347 FYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQG 386

BLAST of Cp4.1LG08g12420 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 423.3 bits (1087), Expect = 1.9e-118
Identity = 200/354 (56.50%), Postives = 263/354 (74.29%), Query Frame = 0

Query: 4   LESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE 63
           ++ WPEP+V VQSLAES + ++P RY+KP S RP+    +     NIP++D+E L S   
Sbjct: 17  MDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPT-TTEDAPTATNIPIIDLEGLFSEEG 76

Query: 64  VT------AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTY 123
           ++       ++EAC+ WGFF V+NHG+  E+M+  ++ W +FFH P++ K  Y+NSP TY
Sbjct: 77  LSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTY 136

Query: 124 EGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEK 183
           EGYGSRLG+EK A LDWSDY+F++ LP  L++  KWP+FPP+ +E+I+EYG+E+VKL  +
Sbjct: 137 EGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGR 196

Query: 184 VMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILL 243
           +M+ LS  LGL+E+   +AFGG E IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILL
Sbjct: 197 IMRVLSTNLGLKEDKFQEAFGG-ENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 256

Query: 244 PDHNVPGLQVRKGDDWITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSNKDRVSL 303
           PD  V GLQVRK D WIT+ P P+A IVNI DQIQ+LSN  YKSV HRVIVNS+K+RVSL
Sbjct: 257 PDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSL 316

Query: 304 AFFYNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRGICGTSQQEQSI 351
           AFFYNP+SD+ I+P + LV+   P L+PP TFD+YRL IR +G  G S  E  I
Sbjct: 317 AFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKSHVESHI 368

BLAST of Cp4.1LG08g12420 vs. TAIR 10
Match: AT3G55970.1 (jasmonate-regulated gene 21 )

HSP 1 Score: 406.4 bits (1043), Expect = 2.4e-113
Identity = 194/349 (55.59%), Postives = 258/349 (73.93%), Query Frame = 0

Query: 1   MSCLESWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPR--NIPVVDMEKL 60
           M+  + WPEP+V VQSL+ES +  IP RYVKP SQRP+     ++ P+   IP++D+ +L
Sbjct: 1   MNIFQDWPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRL 60

Query: 61  QS------AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYAN 120
            +      A  +  +++AC+E GFF V+NHG++ ++M+  K  W +FF+ P++LK  +AN
Sbjct: 61  YTDDLTLQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHAN 120

Query: 121 SPTTYEGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFP-PSKELIEEYGKEMV 180
           SP TYEGYGSRLG+EK AILDWSDY++++Y P SL++  KWP+ P   +E++E+Y KEMV
Sbjct: 121 SPKTYEGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMV 180

Query: 181 KLCEKVMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 240
           KLCE +MK LS  LGL+E+ L  AFGG+E  G C+R N YPKCPQP+LTLG+SPHSDPGG
Sbjct: 181 KLCENLMKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGG 240

Query: 241 ITILLPDHNVPGLQVRKGDD-WITIDPIPNALIVNIADQIQVLSNGIYKSVNHRVIVNSN 300
           +TILLPD  V  LQVR  DD WIT++P P+A IVN+ DQIQ+LSN IYKSV HRVIVN  
Sbjct: 241 LTILLPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPE 300

Query: 301 KDRVSLAFFYNPRSDLIIEPAKVLVTEERPALFPPTTFDEYRLQIRKRG 340
            +R+SLAFFYNP+ ++ IEP K LVT + PAL+  TT+D YR  IR +G
Sbjct: 301 NERLSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQG 349