Cp4.1LG08g08230 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG08g08230 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 2.6e-201
Identity = 370/741 (49.93%), Postives = 477/741 (64.37%), Query Frame = 0

Query: 14  YIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSYNRHINGFAAMLD 73
           Y+VY G+ SH    +     RV E+HY  L S +GS+  A +AI +SY +HINGFAA LD
Sbjct: 32  YVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHLD 91

Query: 74  ENQASELA---------------------------------------------------- 133
            + A E++                                                    
Sbjct: 92  HDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIAN 151

Query: 134 --------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGY----GILN 193
                   +KSF DEG GPIPSRWKG CQ+  D  FHCN+KLIGAR+FNKGY    G LN
Sbjct: 152 LDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLN 211

Query: 194 ATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCW-PVEDR 253
           ++F+SPRD +GHG+HTLS A G+FV G ++F   NGTAKGGSPRAR+A+YKVCW PV+  
Sbjct: 212 SSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGN 271

Query: 254 QCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFG 313
           +C D + LAA+DAAI DG DVISVS+GGEP  F  D++++G+FHA +  IVVVCSAGN G
Sbjct: 272 ECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSG 331

Query: 314 PTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVD 373
           P  +TVSNV+PW +TVGAST+DR+F + +VLGN K  KG S SS AL   KFYP++ +V+
Sbjct: 332 PADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVN 391

Query: 374 AKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKD 433
           AKA NAS+ DA++C   SLDP K  GKI+VCLRG   RV KG  VA  G  GM+L N   
Sbjct: 392 AKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYV 451

Query: 434 NGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSR 493
            G+ +  D H+LPA+ +T  D  ++ +YI  TK P+A I+  +T+L + P+PVMA FSS+
Sbjct: 452 TGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSK 511

Query: 494 GPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIV 553
           GPS +   ILKPDITAPGV++IAAY   +     Q D RR  F   SGTSM+CPH++GI 
Sbjct: 512 GPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIA 571

Query: 554 GLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPG 613
           GLLK+RYP WSPAAI+SAIMTTA    +   PI + T +KATP ++G GHV PN  ++PG
Sbjct: 572 GLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPG 631

Query: 614 LVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQ-SFKVTDLNYPSISVTDIKTGPVT 673
           LVYD+ I DYLNFLC+ G NA QI+  S   F C      + +LNYPSI+V ++ +  VT
Sbjct: 632 LVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVT 691

Query: 674 VNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYA 689
           V+R +KNVG    Y  +V  P  V +AV+P++L FT + E+K+FK++L +S     +GY 
Sbjct: 692 VSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV 751

BLAST of Cp4.1LG08g08230 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 3.6e-195
Identity = 370/754 (49.07%), Postives = 478/754 (63.40%), Query Frame = 0

Query: 2   LFQTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSY 61
           LF +   A +K YIVYLGS +H    SS H   V  SH + L S  GS   A+EAI +SY
Sbjct: 30  LFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSY 89

Query: 62  NRHINGFAAMLDENQASELA---------------------------------------- 121
            RHINGFAA+LDEN+A+E+A                                        
Sbjct: 90  KRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWN 149

Query: 122 --------------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFF 181
                               +KSFSDEGYG +P+RWKG C  D      CN+KLIGAR+F
Sbjct: 150 KAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKD----VPCNRKLIGARYF 209

Query: 182 NKGY----GI-LNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARI 241
           NKGY    G+  NA++ + RD +GHG+HTLS A GNFV GANVF + NGTA GGSP+AR+
Sbjct: 210 NKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARV 269

Query: 242 ASYKVCW-PVEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQ 301
           A+YKVCW PV+  +C D + LAA +AAI DGVDV+S S+GG+  +++ D +++G+FHAV+
Sbjct: 270 AAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVK 329

Query: 302 HGIVVVCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKAL 361
           +G+ VVCSAGN GP   TVSNV+PWV+TVGAS++DR+F  FV L N +  KGTS  SK L
Sbjct: 330 NGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL-SKPL 389

Query: 362 AFNKFYPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQ 421
              K Y LI+A DA   N + +DA +C + SLDP K+ GKI+VCLRG  +RV KG   A 
Sbjct: 390 PEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAA 449

Query: 422 AGAAGMILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELD 481
           AGAAGM+L NDK +G+ I +D H+LPAS + + DG ++F Y+ STK P   I +    L+
Sbjct: 450 AGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLN 509

Query: 482 VNPSPVMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDS 541
             P+P MA FSSRGP+TI   ILKPDITAPGVNIIAA+ +     +L  D+RR PF  +S
Sbjct: 510 TKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTES 569

Query: 542 GTSMACPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYG 601
           GTSM+CPH++G+VGLLK+ +P WSPAAI+SAIMTT++T  N   P++D +  KA P +YG
Sbjct: 570 GTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYG 629

Query: 602 NGHVDPNNVMDPGLVYDISIDDYLNFLCARGENAIQINKLSH-KLFVCDQSFKVTDLNYP 661
           +GHV PN    PGLVYD++  DYL+FLCA G N   +   +    + C Q   + D NYP
Sbjct: 630 SGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYP 689

Query: 662 SISVTDIKTGPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIV 689
           SI+V ++ TG +TV RKLKNVG   TY A+ + PL V ++VEP  L F    E K F++ 
Sbjct: 690 SITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMT 749

BLAST of Cp4.1LG08g08230 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 590.1 bits (1520), Expect = 3.2e-167
Identity = 344/761 (45.20%), Postives = 461/761 (60.58%), Query Frame = 0

Query: 10  TRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSYNRHINGFA 69
           ++K YIVY+G+ SHG S +S   +  T+SHY LL S+ GS+  A+EAI +SYNRHINGFA
Sbjct: 28  SKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFA 87

Query: 70  AMLDENQASELA------------------------------------------------ 129
           A+L+E +A+++A                                                
Sbjct: 88  ALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRGQNSAWQKGRFGENTIIG 147

Query: 130 ---------TKSFSDEGYGPIPSRWK-GTCQSDSDPNF---HCNKKLIGARFFNKGY--- 189
                    ++SFSD+GYG +PS+W+ G CQ +  P      CN+KLIGAR++NK +   
Sbjct: 148 NIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAH 207

Query: 190 -GILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWP 249
            G L+   ++ RD  GHGTHTLS AGGNFV GA VFA+ NGTAKGGSPRAR+A+YKVCW 
Sbjct: 208 NGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWS 267

Query: 250 VED-RQCLDPNALAAYDAAISDGVDVISVSIGGE----PKEFLRDALSVGAFHAVQHGIV 309
           + D   C   + LAA D AI DGVDVI+VS G       +    D +S+GAFHA+   I+
Sbjct: 268 LTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNIL 327

Query: 310 VVCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNK 369
           +V SAGN GPTP TV+NV+PWV T+ AST+DRDF++ + + N + ++G S     L  N+
Sbjct: 328 LVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTI-NNQLIEGASLFVN-LPPNQ 387

Query: 370 FYPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLR-GVISRVSKGYVVAQAGA 429
            + LI + DAK  NA+  DA++C   +LD TK+ GKIV+C R G I  V++G     AGA
Sbjct: 388 AFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGA 447

Query: 430 AGMILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKST-----KTPMAC-----IS 489
            GMIL N   NG  ++ + H+    +       S    +K+T       P+       +S
Sbjct: 448 RGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMS 507

Query: 490 SVKTELDVNPSPVMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRR 549
             +T     P+PVMA FSSRGP+ I+ SILKPD+TAPGVNI+AAY +      L VD+RR
Sbjct: 508 RARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRR 567

Query: 550 A-PFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILD-FTG 609
              F V  GTSM+CPH +GI GLLK+R+P WSPAAIKSAIMTTA T  N   PI D F  
Sbjct: 568 GFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDK 627

Query: 610 LKATPLAYGNGHVDPNNVMDPGLVYDISIDDYLNFLCARGENAIQINKLS-HKLFVCDQS 669
             A   AYG+GHV P+  ++PGLVYD+S+ DYLNFLCA G +   I+ L+ ++ F+C  S
Sbjct: 628 TLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGS 687

Query: 670 FKVTDLNYPSISVTDIKTGPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAM 687
             V DLNYPSI++ +++  PVT+ R + NVG   TY    ++P   SIAV P +L FT +
Sbjct: 688 HSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKI 747

BLAST of Cp4.1LG08g08230 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 5.3e-146
Identity = 285/620 (45.97%), Postives = 392/620 (63.23%), Query Frame = 0

Query: 82  TKSFSDEGYGPIPSRWKGTCQSDSDPNFH---CNKKLIGARFFNKGY----GILNAT--F 141
           +KS+SDEG+GPIPS WKG C++ +  NF    CN+KLIGARFF +GY    G ++ +   
Sbjct: 146 SKSYSDEGFGPIPSSWKGGCEAGT--NFTASLCNRKLIGARFFARGYESTMGPIDESKES 205

Query: 142 NSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLD 201
            SPRD +GHGTHT S A G+ V GA++   A+GTA+G +PRAR+A YKVCW      C  
Sbjct: 206 RSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL---GGCFS 265

Query: 202 PNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPA 261
            + LAA D AI+D V+V+S+S+GG   ++ RD +++GAF A++ GI+V CSAGN GP+ +
Sbjct: 266 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 325

Query: 262 TVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTS-FSSKALAFNKFYPLINAVDAKA 321
           ++SNV+PW+ TVGA T+DRDF    +LGN K   G S F  +AL  +K  P I A     
Sbjct: 326 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP-DKLLPFIYA----G 385

Query: 322 NNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGD 381
           N +++++  +C   +L P K+ GKIV+C RG+ +RV KG VV  AG  GMIL N   NG+
Sbjct: 386 NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGE 445

Query: 382 AIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPS 441
            +  D HLLPA+ V    G  I  Y+ +   P A IS + T + V PSPV+A FSSRGP+
Sbjct: 446 ELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPN 505

Query: 442 TIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLL 501
           +I  +ILKPD+ APGVNI+AA+        L  D RR  F + SGTSM+CPHV+G+  LL
Sbjct: 506 SITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALL 565

Query: 502 KSRYPKWSPAAIKSAIMTTAKTNANNFNPILDF-TGLKATPLAYGNGHVDPNNVMDPGLV 561
           KS +P+WSPAAI+SA+MTTA     +  P+LD  TG  +TP  +G GHV P    +PGL+
Sbjct: 566 KSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLI 625

Query: 562 YDISIDDYLNFLCARGENAIQINKLSHKLFVCD--QSFKVTDLNYPSISVTDIKTGPVTV 621
           YD++ +DYL FLCA    + QI  +S + + CD  +S+ V DLNYPS +V     G    
Sbjct: 626 YDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKY 685

Query: 622 NRKLKNVGSLGTYVAQVKAPLE-VSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYA 681
            R + +VG  GTY  +V +    V I+VEP+ L F   +E+KS+ +             +
Sbjct: 686 TRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNS 745

Query: 682 FGELAWSDGKHNVRSSIAVN 688
           FG + WSDGKH V S +A++
Sbjct: 746 FGSIEWSDGKHVVGSPVAIS 755

BLAST of Cp4.1LG08g08230 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.9e-135
Identity = 277/631 (43.90%), Postives = 381/631 (60.38%), Query Frame = 0

Query: 84  SFSDEGYGPIPSRWKGTCQSDSD-PNFHCNKKLIGARFFNKGY----GILNAT--FNSPR 143
           SF D G GP+P +WKG C +  D P   CN+KL+GARFF  GY    G +N T  F SPR
Sbjct: 146 SFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPR 205

Query: 144 DQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLDPNAL 203
           D +GHGTHT SI+ G +V  A+    A+G A G +P+AR+A+YKVCW   +  C D + L
Sbjct: 206 DSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW---NSGCYDSDIL 265

Query: 204 AAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSN 263
           AA+D A++DGVDVIS+S+GG    +  DA+++GAF A+  GI V  SAGN GP   TV+N
Sbjct: 266 AAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTN 325

Query: 264 VSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTS-FSSKALAFNKFYPLINAVDAKANNAS 323
           V+PW+ TVGA TIDRDF   V LGN K + G S +    L   + YPL+        +  
Sbjct: 326 VAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY 385

Query: 324 SSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIAT 383
           SS   +C E SLDP  + GKIV+C RG+ SR +KG +V + G  GMI+ N   +G+ +  
Sbjct: 386 SS--SLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVA 445

Query: 384 DLHLLPASHVTFNDGISIFQYI------KSTKTPMACISSVKTELDVNPSPVMADFSSRG 443
           D H+LPA+ V  + G  I +YI      +S+K P A I    T L + P+PV+A FS+RG
Sbjct: 446 DCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARG 505

Query: 444 PSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVG 503
           P+     ILKPD+ APG+NI+AA+PD I    +  D+RR  F + SGTSMACPHV+G+  
Sbjct: 506 PNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAA 565

Query: 504 LLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILD-FTGLKATPLAYGNGHVDPNNVMDPG 563
           LLK+ +P WSPAAI+SA++TTA T  N+  P++D  TG  ++ + YG+GHV P   MDPG
Sbjct: 566 LLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPG 625

Query: 564 LVYDISIDDYLNFLCARGENAIQINKLSHKLFVCD---QSFKVTDLNYPSISVTDIKTGP 623
           LVYDI+  DY+NFLC        I  ++ +   CD   ++  V +LNYPS SV   + G 
Sbjct: 626 LVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGE 685

Query: 624 VTVN----RKLKNVG-SLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 683
             ++    R + NVG S   Y  +++ P   ++ VEP  L F  + ++ SF + ++ +  
Sbjct: 686 SKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEV 745

Query: 684 GSQEG---YAFGELAWSDGKHNVRSSIAVNL 689
               G      G + WSDGK NV S + V L
Sbjct: 746 KLSPGATNVETGHIVWSDGKRNVTSPLVVTL 771

BLAST of Cp4.1LG08g08230 vs. NCBI nr
Match: KAG6596775.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1315 bits (3403), Expect = 0.0
Identity = 671/750 (89.47%), Postives = 681/750 (90.80%), Query Frame = 0

Query: 1   MLFQTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHS 60
           MLFQTCIIAT+KPYIVYLGSLSHGSSTSSLHHQRVTESHY+LL+SVSGSKNIAEEAISHS
Sbjct: 17  MLFQTCIIATKKPYIVYLGSLSHGSSTSSLHHQRVTESHYNLLESVSGSKNIAEEAISHS 76

Query: 61  YNRHINGFAAMLDENQASELA--------------------------------------- 120
            NRHINGFAAMLDENQAS+LA                                       
Sbjct: 77  CNRHINGFAAMLDENQASKLAKFPDVVSVFECQARTLHTTRSWNFLGMEKHEGIPLNSIW 136

Query: 121 ---------------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARF 180
                                 KSFSDEGYGPIPSRWKGTCQSD DPNFHCNKKLIGARF
Sbjct: 137 NFARFGDDTIIANFDSGVWPEAKSFSDEGYGPIPSRWKGTCQSDLDPNFHCNKKLIGARF 196

Query: 181 FNKGYGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYK 240
           FNKGYGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYK
Sbjct: 197 FNKGYGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYK 256

Query: 241 VCWPVEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV 300
           VCWPVE RQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV
Sbjct: 257 VCWPVEGRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV 316

Query: 301 VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKF 360
           VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGN KKLKGTSFSSKALAFNKF
Sbjct: 317 VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNNKKLKGTSFSSKALAFNKF 376

Query: 361 YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG 420
           YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG
Sbjct: 377 YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG 436

Query: 421 MILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSP 480
           MILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSP
Sbjct: 437 MILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSP 496

Query: 481 VMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA 540
           VMADFSSRGPSTIE SILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA
Sbjct: 497 VMADFSSRGPSTIEESILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA 556

Query: 541 CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVD 600
           CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGL+ATPLAYGNGHVD
Sbjct: 557 CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLEATPLAYGNGHVD 616

Query: 601 PNNVMDPGLVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTD 660
           PN+VMDPGL+YDISIDDYLNFLCARGENA QINKLSHKLFVCD SFKVTDLNYPSISVT+
Sbjct: 617 PNSVMDPGLIYDISIDDYLNFLCARGENATQINKLSHKLFVCDPSFKVTDLNYPSISVTN 676

Query: 661 IKTGPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 690
           +KTGPVT+NRKLKNVGS GTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK
Sbjct: 677 LKTGPVTINRKLKNVGSPGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 736

BLAST of Cp4.1LG08g08230 vs. NCBI nr
Match: XP_023005316.1 (subtilisin-like protease SBT5.3 [Cucurbita maxima])

HSP 1 Score: 1308 bits (3384), Expect = 0.0
Identity = 667/750 (88.93%), Postives = 678/750 (90.40%), Query Frame = 0

Query: 1   MLFQTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHS 60
           MLFQTC IAT+K YIVYLGSLSHGSSTSSLHHQRVTESHY+LL+ VSGSKNIAEEAISHS
Sbjct: 17  MLFQTCTIATKKSYIVYLGSLSHGSSTSSLHHQRVTESHYNLLELVSGSKNIAEEAISHS 76

Query: 61  YNRHINGFAAMLDENQASELA--------------------------------------- 120
           YNRHINGFAAMLDENQ SELA                                       
Sbjct: 77  YNRHINGFAAMLDENQVSELAKFPDVVSVFECQARTLHTTRSWNFLGMEKHEGIPSTSIW 136

Query: 121 ---------------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARF 180
                                 KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARF
Sbjct: 137 NIARFGGDTIIANFDSGVWPEAKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARF 196

Query: 181 FNKGYGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYK 240
           FNKGYG LNATFNSPRDQEGHGTHTLSIAGGNFVSGANVF MANGTAKGGSPRARIASYK
Sbjct: 197 FNKGYGNLNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFDMANGTAKGGSPRARIASYK 256

Query: 241 VCWPVEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV 300
           VCWPVE RQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV
Sbjct: 257 VCWPVEGRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV 316

Query: 301 VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKF 360
           VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKF
Sbjct: 317 VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKF 376

Query: 361 YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG 420
           YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG
Sbjct: 377 YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG 436

Query: 421 MILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSP 480
           MILVNDKDNGDAIATDLHLLPASHVTFNDGISIF YIKSTKTPMACISSVKTELDVNPSP
Sbjct: 437 MILVNDKDNGDAIATDLHLLPASHVTFNDGISIFHYIKSTKTPMACISSVKTELDVNPSP 496

Query: 481 VMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA 540
           VMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA
Sbjct: 497 VMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA 556

Query: 541 CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVD 600
           CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKT+ANNFNPILDFTGL+ATPLAYGNGHVD
Sbjct: 557 CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTSANNFNPILDFTGLEATPLAYGNGHVD 616

Query: 601 PNNVMDPGLVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTD 660
           PN+VMDPGLVYDI+IDDYLNFLCARG+NA QINKLSHKLFVCD SFKVTDLNYPSISVT+
Sbjct: 617 PNSVMDPGLVYDIAIDDYLNFLCARGDNATQINKLSHKLFVCDPSFKVTDLNYPSISVTN 676

Query: 661 IKTGPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 690
           +KTGPVT+NRKLKNVGS GTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK
Sbjct: 677 LKTGPVTINRKLKNVGSPGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 736

BLAST of Cp4.1LG08g08230 vs. NCBI nr
Match: XP_023540457.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1219 bits (3155), Expect = 0.0
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0

Query: 83  KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG 142
           KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG
Sbjct: 73  KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG 132

Query: 143 THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLDPNALAAYDAA 202
           THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLDPNALAAYDAA
Sbjct: 133 THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLDPNALAAYDAA 192

Query: 203 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 262
           ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL
Sbjct: 193 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 252

Query: 263 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC 322
           TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC
Sbjct: 253 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC 312

Query: 323 NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA 382
           NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA
Sbjct: 313 NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA 372

Query: 383 SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI 442
           SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI
Sbjct: 373 SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI 432

Query: 443 TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA 502
           TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA
Sbjct: 433 TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA 492

Query: 503 IKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPGLVYDISIDDYLNFL 562
           IKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPGLVYDISIDDYLNFL
Sbjct: 493 IKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPGLVYDISIDDYLNFL 552

Query: 563 CARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKTGPVTVNRKLKNVGSLGTYV 622
           CARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKTGPVTVNRKLKNVGSLGTYV
Sbjct: 553 CARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKTGPVTVNRKLKNVGSLGTYV 612

Query: 623 AQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYAFGELAWSDGKHNVRS 682
           AQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYAFGELAWSDGKHNVRS
Sbjct: 613 AQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYAFGELAWSDGKHNVRS 672

Query: 683 SIAVNLGK 690
           SIAVNLGK
Sbjct: 673 SIAVNLGK 680

BLAST of Cp4.1LG08g08230 vs. NCBI nr
Match: XP_022946325.1 (subtilisin-like protease SBT5.3 [Cucurbita moschata])

HSP 1 Score: 1199 bits (3103), Expect = 0.0
Identity = 596/608 (98.03%), Postives = 603/608 (99.18%), Query Frame = 0

Query: 83  KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG 142
           KSFSDEGYGPIPSRWKGTCQSD DPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG
Sbjct: 73  KSFSDEGYGPIPSRWKGTCQSDLDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG 132

Query: 143 THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLDPNALAAYDAA 202
           THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVE RQCLDPNALAAYDAA
Sbjct: 133 THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEGRQCLDPNALAAYDAA 192

Query: 203 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 262
           ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL
Sbjct: 193 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 252

Query: 263 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC 322
           TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC
Sbjct: 253 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC 312

Query: 323 NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA 382
           NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA
Sbjct: 313 NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA 372

Query: 383 SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI 442
           SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI
Sbjct: 373 SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI 432

Query: 443 TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA 502
           TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA
Sbjct: 433 TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA 492

Query: 503 IKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPGLVYDISIDDYLNFL 562
           IKSAIMTTAKTNANNFNPILDFTGL+ATPLAYGNGHVDPN+VMDPGLVYDISIDDYLNFL
Sbjct: 493 IKSAIMTTAKTNANNFNPILDFTGLEATPLAYGNGHVDPNSVMDPGLVYDISIDDYLNFL 552

Query: 563 CARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKTGPVTVNRKLKNVGSLGTYV 622
           CARGENA QINKLSHKLFVCD SFKVTDLNYPSISVT++KTGPVT+NRKLKNVGS GTY+
Sbjct: 553 CARGENATQINKLSHKLFVCDPSFKVTDLNYPSISVTNLKTGPVTINRKLKNVGSPGTYI 612

Query: 623 AQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYAFGELAWSDGKHNVRS 682
           AQVKAPLEVSIAVEPSTLQFTAMDEEKSFK+VLQRSGKGSQEGYAFGELAWSDGKHNVRS
Sbjct: 613 AQVKAPLEVSIAVEPSTLQFTAMDEEKSFKMVLQRSGKGSQEGYAFGELAWSDGKHNVRS 672

Query: 683 SIAVNLGK 690
           SIAVNLGK
Sbjct: 673 SIAVNLGK 680

BLAST of Cp4.1LG08g08230 vs. NCBI nr
Match: XP_038903594.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1080 bits (2792), Expect = 0.0
Identity = 546/749 (72.90%), Postives = 606/749 (80.91%), Query Frame = 0

Query: 2   LFQTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSY 61
           LFQTC IA +KPYIVYLGS SHG S SSL H+R T SHY LL SV GSK IA+EAI +SY
Sbjct: 18  LFQTCTIANKKPYIVYLGSHSHGLSASSLDHRRATVSHYDLLGSVLGSKMIAKEAILYSY 77

Query: 62  NRHINGFAAMLDENQASELA---------------------------------------- 121
           N+HINGFAAMLDE QA++LA                                        
Sbjct: 78  NKHINGFAAMLDEKQAADLAKFPNVVSVFESQARKLHTTRSWKFLGIEKHEEIPSNYIWN 137

Query: 122 --------------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFF 181
                               +KSFSDEGYGPIPSRWKGTCQSD  P FHCN+KLIGARFF
Sbjct: 138 VARFGDDIIIANFDTGVWPESKSFSDEGYGPIPSRWKGTCQSD--PKFHCNRKLIGARFF 197

Query: 182 NKGYGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKV 241
           N GYG L+ TFNS +D  GHGTHTLSIAGGNFVSGANV  M NGT KGGSPRAR+ASYKV
Sbjct: 198 NLGYGELSVTFNSSKDNVGHGTHTLSIAGGNFVSGANVLGMGNGTIKGGSPRARVASYKV 257

Query: 242 CWPVEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVV 301
           CWP E  +CLDPN LAA++AAI DGVDVIS+S+G EPKEF  DALSVGAFHAV+ GIVVV
Sbjct: 258 CWPAEASECLDPNTLAAFEAAIDDGVDVISISVGAEPKEFFSDALSVGAFHAVERGIVVV 317

Query: 302 CSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFY 361
           CSAGN GPTP TVSNVSPW+LTVGASTIDRDFTNFVVLGNKKK KGTSFSSKAL FNK Y
Sbjct: 318 CSAGNVGPTPGTVSNVSPWILTVGASTIDRDFTNFVVLGNKKKFKGTSFSSKALPFNKLY 377

Query: 362 PLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGM 421
           PLINAVDAKANN S+SDAEVC E SLDP KL GKIVVCLRG + RVSKGYV A+AGAAGM
Sbjct: 378 PLINAVDAKANNVSNSDAEVCEEGSLDPEKLTGKIVVCLRGGLPRVSKGYVAAKAGAAGM 437

Query: 422 ILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPV 481
           ILVND+++G+AI TDLH+LPASH+T+ND ISIFQYI STKTPMA ISSV TEL++ PSPV
Sbjct: 438 ILVNDEESGNAILTDLHILPASHITYNDSISIFQYINSTKTPMAYISSVMTELEIKPSPV 497

Query: 482 MADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMAC 541
           MADFSSRGP+TIE SILKPDITAPGVNI+AAYP+ +PL +L +DDR+APF VDSGTSMAC
Sbjct: 498 MADFSSRGPNTIEESILKPDITAPGVNIMAAYPNGVPLTKLPLDDRQAPFNVDSGTSMAC 557

Query: 542 PHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDP 601
           PHVAGIVGLLK+  PKWSPAAIKSAIMTTAKT  N+ +PILD TGLKATPLAYG GHV+P
Sbjct: 558 PHVAGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDNSLHPILDSTGLKATPLAYGAGHVNP 617

Query: 602 NNVMDPGLVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDI 661
           N+ MDPGLVYDI+IDDYLNFLCARG NA QI ++S K+FVCD+SFKVTDLNYPSISVT +
Sbjct: 618 NSAMDPGLVYDITIDDYLNFLCARGYNATQIKRISKKIFVCDRSFKVTDLNYPSISVTGL 677

Query: 662 KTGPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKG 690
           KTG VT+NRK+KNVGS GTYVA+VKAPLEVSI VEPSTL FTA+DEEKSFK++L  +GKG
Sbjct: 678 KTGHVTINRKVKNVGSPGTYVARVKAPLEVSIMVEPSTLHFTAIDEEKSFKVLLHSTGKG 737

BLAST of Cp4.1LG08g08230 vs. ExPASy TrEMBL
Match: A0A6J1KUM0 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498350 PE=3 SV=1)

HSP 1 Score: 1308 bits (3384), Expect = 0.0
Identity = 667/750 (88.93%), Postives = 678/750 (90.40%), Query Frame = 0

Query: 1   MLFQTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHS 60
           MLFQTC IAT+K YIVYLGSLSHGSSTSSLHHQRVTESHY+LL+ VSGSKNIAEEAISHS
Sbjct: 17  MLFQTCTIATKKSYIVYLGSLSHGSSTSSLHHQRVTESHYNLLELVSGSKNIAEEAISHS 76

Query: 61  YNRHINGFAAMLDENQASELA--------------------------------------- 120
           YNRHINGFAAMLDENQ SELA                                       
Sbjct: 77  YNRHINGFAAMLDENQVSELAKFPDVVSVFECQARTLHTTRSWNFLGMEKHEGIPSTSIW 136

Query: 121 ---------------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARF 180
                                 KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARF
Sbjct: 137 NIARFGGDTIIANFDSGVWPEAKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARF 196

Query: 181 FNKGYGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYK 240
           FNKGYG LNATFNSPRDQEGHGTHTLSIAGGNFVSGANVF MANGTAKGGSPRARIASYK
Sbjct: 197 FNKGYGNLNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFDMANGTAKGGSPRARIASYK 256

Query: 241 VCWPVEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV 300
           VCWPVE RQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV
Sbjct: 257 VCWPVEGRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVV 316

Query: 301 VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKF 360
           VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKF
Sbjct: 317 VCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKF 376

Query: 361 YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG 420
           YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG
Sbjct: 377 YPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAG 436

Query: 421 MILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSP 480
           MILVNDKDNGDAIATDLHLLPASHVTFNDGISIF YIKSTKTPMACISSVKTELDVNPSP
Sbjct: 437 MILVNDKDNGDAIATDLHLLPASHVTFNDGISIFHYIKSTKTPMACISSVKTELDVNPSP 496

Query: 481 VMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA 540
           VMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA
Sbjct: 497 VMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMA 556

Query: 541 CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVD 600
           CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKT+ANNFNPILDFTGL+ATPLAYGNGHVD
Sbjct: 557 CPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTSANNFNPILDFTGLEATPLAYGNGHVD 616

Query: 601 PNNVMDPGLVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTD 660
           PN+VMDPGLVYDI+IDDYLNFLCARG+NA QINKLSHKLFVCD SFKVTDLNYPSISVT+
Sbjct: 617 PNSVMDPGLVYDIAIDDYLNFLCARGDNATQINKLSHKLFVCDPSFKVTDLNYPSISVTN 676

Query: 661 IKTGPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 690
           +KTGPVT+NRKLKNVGS GTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK
Sbjct: 677 LKTGPVTINRKLKNVGSPGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 736

BLAST of Cp4.1LG08g08230 vs. ExPASy TrEMBL
Match: A0A6J1G3J4 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111450435 PE=3 SV=1)

HSP 1 Score: 1199 bits (3103), Expect = 0.0
Identity = 596/608 (98.03%), Postives = 603/608 (99.18%), Query Frame = 0

Query: 83  KSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG 142
           KSFSDEGYGPIPSRWKGTCQSD DPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG
Sbjct: 73  KSFSDEGYGPIPSRWKGTCQSDLDPNFHCNKKLIGARFFNKGYGILNATFNSPRDQEGHG 132

Query: 143 THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLDPNALAAYDAA 202
           THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVE RQCLDPNALAAYDAA
Sbjct: 133 THTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEGRQCLDPNALAAYDAA 192

Query: 203 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 262
           ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL
Sbjct: 193 ISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVL 252

Query: 263 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC 322
           TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC
Sbjct: 253 TVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVC 312

Query: 323 NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA 382
           NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA
Sbjct: 313 NERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPA 372

Query: 383 SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI 442
           SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI
Sbjct: 373 SHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDI 432

Query: 443 TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA 502
           TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA
Sbjct: 433 TAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAA 492

Query: 503 IKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPGLVYDISIDDYLNFL 562
           IKSAIMTTAKTNANNFNPILDFTGL+ATPLAYGNGHVDPN+VMDPGLVYDISIDDYLNFL
Sbjct: 493 IKSAIMTTAKTNANNFNPILDFTGLEATPLAYGNGHVDPNSVMDPGLVYDISIDDYLNFL 552

Query: 563 CARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKTGPVTVNRKLKNVGSLGTYV 622
           CARGENA QINKLSHKLFVCD SFKVTDLNYPSISVT++KTGPVT+NRKLKNVGS GTY+
Sbjct: 553 CARGENATQINKLSHKLFVCDPSFKVTDLNYPSISVTNLKTGPVTINRKLKNVGSPGTYI 612

Query: 623 AQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYAFGELAWSDGKHNVRS 682
           AQVKAPLEVSIAVEPSTLQFTAMDEEKSFK+VLQRSGKGSQEGYAFGELAWSDGKHNVRS
Sbjct: 613 AQVKAPLEVSIAVEPSTLQFTAMDEEKSFKMVLQRSGKGSQEGYAFGELAWSDGKHNVRS 672

Query: 683 SIAVNLGK 690
           SIAVNLGK
Sbjct: 673 SIAVNLGK 680

BLAST of Cp4.1LG08g08230 vs. ExPASy TrEMBL
Match: A0A5D3D737 (Subtilisin-like protease SBT5.3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00080 PE=3 SV=1)

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 519/740 (70.14%), Postives = 579/740 (78.24%), Query Frame = 0

Query: 12  KPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSYNRHINGFAAM 71
           KPYIVYLGS SHG STS L H+R T SHY LL S  GSK IA+E I +SYN++INGF AM
Sbjct: 9   KPYIVYLGSHSHGWSTSPLDHRRATTSHYDLLGSALGSKKIAKEVILYSYNKNINGFVAM 68

Query: 72  LDENQASELA-------------------------------------------------- 131
           LDE QA++LA                                                  
Sbjct: 69  LDERQATDLAKFPHVVSVFESRSRKLHTTQSWKFLGVEKHEEIPTSNSIWNVTRFGEDII 128

Query: 132 -----------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGYGILNA 191
                      +KSFSDEGYGPIPSRW GTCQSD+DP F CN+KLIGARFFN GYG L  
Sbjct: 129 IANFDTGVWPESKSFSDEGYGPIPSRWMGTCQSDADPKFRCNRKLIGARFFNIGYGELAD 188

Query: 192 TFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQC 251
           TFNS RD  GHGTHTLSIAGGNFV GANV  + NGT KGGSPRAR+ASYKVCWP E  +C
Sbjct: 189 TFNSSRDNVGHGTHTLSIAGGNFVPGANVLGLGNGTVKGGSPRARVASYKVCWPDEANEC 248

Query: 252 LDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPT 311
           +DPN LAA++AAI DGVDVIS+S+GGEPKEF  DALSVGAFHAV+ GIVVV SAGN GPT
Sbjct: 249 MDPNTLAAFEAAIDDGVDVISISVGGEPKEFFSDALSVGAFHAVERGIVVVSSAGNMGPT 308

Query: 312 PATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAK 371
           P TVSNVSPW+LTVGAST DR FTNFV+LGNKKK KGTSFSSK L  NKFYPLINAVDAK
Sbjct: 309 PGTVSNVSPWILTVGASTTDRGFTNFVILGNKKKFKGTSFSSKVLPVNKFYPLINAVDAK 368

Query: 372 ANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNG 431
           A N S SDAEVC+E SLDP KLAGKIVVCLRG +SRVSKGYV A+AGA GMI+ ND+D+G
Sbjct: 369 AKNVSVSDAEVCDEGSLDPKKLAGKIVVCLRGGLSRVSKGYVAAKAGAVGMIVENDEDSG 428

Query: 432 DAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGP 491
           +AI TD H+LPASHVT++D ISIFQYI STK P A ISSV TEL++ PS V+ADFSSRGP
Sbjct: 429 NAIITDSHVLPASHVTYDDSISIFQYINSTKRPKAYISSVMTELEITPSAVVADFSSRGP 488

Query: 492 STIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGL 551
           +TIE SILKPDITAPGVNI+AAYPD IPL E  +DDR++PFKVDSGTSMACPHVAGIVGL
Sbjct: 489 NTIEESILKPDITAPGVNILAAYPDGIPLTEAPLDDRQSPFKVDSGTSMACPHVAGIVGL 548

Query: 552 LKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPGLV 611
           LK+  PKWSPAAIKSAIMTTAKT  NNFNPI+D  GL+A PLAYG GHV+PN+ MDPGLV
Sbjct: 549 LKTLNPKWSPAAIKSAIMTTAKTTDNNFNPIVDHRGLEANPLAYGAGHVNPNSAMDPGLV 608

Query: 612 YDISIDDYLNFLCARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKTGPVTVNR 671
           YDI+IDDYLNFLCARG N  QI ++S K F+CD+SFKVTDLNYPSISVT++K GPV +NR
Sbjct: 609 YDITIDDYLNFLCARGYNTKQIKRISKKNFICDKSFKVTDLNYPSISVTNLKMGPVAINR 668

Query: 672 KLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYAFGE 690
           KLKNVGS G YVA+VK PLEVSI VEP  L+FTAMDEEKSFK++L+RSGKG QEGY FGE
Sbjct: 669 KLKNVGSPGRYVARVKTPLEVSIIVEPRILEFTAMDEEKSFKVLLKRSGKGKQEGYVFGE 728

BLAST of Cp4.1LG08g08230 vs. ExPASy TrEMBL
Match: A0A6J1EWY3 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111438777 PE=3 SV=1)

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 505/746 (67.69%), Postives = 577/746 (77.35%), Query Frame = 0

Query: 4   QTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSYNR 63
           QT  IAT+KPYIVYLGS SHGSS SSL  Q VT+SHY LL SV GSK IA+EAI +SYNR
Sbjct: 20  QTSTIATKKPYIVYLGSHSHGSSPSSLDLQLVTKSHYDLLGSVLGSKEIAKEAIFYSYNR 79

Query: 64  HINGFAAMLDENQASELA------------------------------------------ 123
           +INGFAAML+E QA++LA                                          
Sbjct: 80  YINGFAAMLNEKQAADLARIPNVISVFENKERQLHTTRSWDFLGLETQGVPSDSIWNVTR 139

Query: 124 -----------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKG 183
                            +KSFSDEGYGP+PSRW G+C+S +DPNFHCN+KLIGARFFN  
Sbjct: 140 FGKDAIIANIDTGVWPESKSFSDEGYGPVPSRWLGSCRSGADPNFHCNRKLIGARFFNLA 199

Query: 184 YGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWP 243
            G LN + NSPRD +GHGTHTLS AGGNFVSGANVF  ANGTAKGGSPRAR+ASYKVCW 
Sbjct: 200 NGPLNGSLNSPRDHQGHGTHTLSTAGGNFVSGANVFGYANGTAKGGSPRARVASYKVCWE 259

Query: 244 VEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSA 303
            E   C D + LAA++AAI DGVDVIS S+G  P +FL D LS+GAFHAVQHGIVVVCSA
Sbjct: 260 AEGGGCFDVDILAAFEAAIGDGVDVISASLGAIPSDFLDDGLSIGAFHAVQHGIVVVCSA 319

Query: 304 GNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLI 363
           GN GP P +VSNVSPW+LTVGASTIDR+FTNF+VLGN KKLKG S SSKAL  +KFYPLI
Sbjct: 320 GNLGPDPTSVSNVSPWMLTVGASTIDREFTNFIVLGNNKKLKGASLSSKALTDDKFYPLI 379

Query: 364 NAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILV 423
           NAVDAKANN     AEVC+E +LDPTKL GKIVVC+ GVI+RV+KGY+ AQAGA GMILV
Sbjct: 380 NAVDAKANNVPELAAEVCDEGTLDPTKLNGKIVVCVVGVIARVTKGYLAAQAGAVGMILV 439

Query: 424 NDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMAD 483
           ND+++G+ I  D H++PASHVT+ND I+I QYI ST+TPMA ISSV  +L V P+P +A 
Sbjct: 440 NDEESGNEIKADPHIIPASHVTYNDSITISQYISSTRTPMAYISSVTAKLGVTPAPTVAR 499

Query: 484 FSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHV 543
           FS RGPS IE SILKPDI APGVNIIAAYPD IPL  L VDDRR PF V SGTSM+CPHV
Sbjct: 500 FSGRGPSIIEESILKPDIIAPGVNIIAAYPDGIPLANLPVDDRRGPFMVYSGTSMSCPHV 559

Query: 544 AGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNV 603
           +GIVGLLK+  PKWSPAAIKSAIMTTAKT  ++ +PI+D  G+ ATPLAYG GHV PN+ 
Sbjct: 560 SGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDSSLHPIVDLDGVIATPLAYGAGHVHPNSA 619

Query: 604 MDPGLVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKTG 663
           MDPGLVYDI+IDDYLNFLC+RG NA Q+ K S+  FVC++SFKVTDLNYPSISV D+KTG
Sbjct: 620 MDPGLVYDITIDDYLNFLCSRGYNATQMKKFSNNTFVCNKSFKVTDLNYPSISVADLKTG 679

Query: 664 PVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQE 690
           PVT+NRK+KNVGS G YVA+V +PLEVSI VEPSTLQF+AMDEEKSF++VLQRSGKG+Q+
Sbjct: 680 PVTINRKVKNVGSPGKYVARVTSPLEVSIVVEPSTLQFSAMDEEKSFRVVLQRSGKGNQQ 739

BLAST of Cp4.1LG08g08230 vs. ExPASy TrEMBL
Match: A0A6J1HN63 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111465152 PE=3 SV=1)

HSP 1 Score: 989 bits (2556), Expect = 0.0
Identity = 506/747 (67.74%), Postives = 574/747 (76.84%), Query Frame = 0

Query: 4   QTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSYNR 63
           QT  IAT+KPYIVYLGS SHG S SSL  Q VT+SHY LL SV GSK IA+EAI +SYNR
Sbjct: 20  QTSTIATKKPYIVYLGSHSHGLSPSSLDLQLVTKSHYDLLGSVLGSKEIAKEAILYSYNR 79

Query: 64  HINGFAAMLDENQASELA------------------------------------------ 123
           +INGFAAML+E QA++LA                                          
Sbjct: 80  YINGFAAMLNEKQATDLARIPNVISVFESKERQLHTTRSWDFLGLETQEAVPSDSIWNVT 139

Query: 124 ------------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNK 183
                             ++SFSDEGYGPIPSRW G+CQS +DPNFHCN+KLIGAR FN 
Sbjct: 140 RFGEDAIIANIDTGVWPESRSFSDEGYGPIPSRWLGSCQSGADPNFHCNRKLIGARSFNL 199

Query: 184 GYGILNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCW 243
             G LN +FNSPRD +GHGTHTLS AGGNFVSGANVF  ANGT+KGGSPRAR+ASYKVCW
Sbjct: 200 ANGPLNGSFNSPRDHQGHGTHTLSTAGGNFVSGANVFGYANGTSKGGSPRARVASYKVCW 259

Query: 244 PVEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCS 303
             E   C D + LAA++AAI DGVDVIS S+G  P +FL D LS+GAFHAVQHGIVVVCS
Sbjct: 260 EAEGGGCFDVDILAAFEAAIGDGVDVISASLGAIPSDFLDDGLSIGAFHAVQHGIVVVCS 319

Query: 304 AGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPL 363
           AGN GP P TVSNVSPW+LTVGA TIDR+FTNFVVLGNKKKLKG S SSKAL  +KFYPL
Sbjct: 320 AGNSGPAPRTVSNVSPWMLTVGAGTIDREFTNFVVLGNKKKLKGASLSSKALKVDKFYPL 379

Query: 364 INAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMIL 423
           INAVDAKANN S  DAE+C E +LD +KL GKIVVCL GV +RV+KGYV AQAGA GMIL
Sbjct: 380 INAVDAKANNVSELDAEICEEGTLDSSKLNGKIVVCLVGVNARVAKGYVAAQAGAIGMIL 439

Query: 424 VNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMA 483
           VND+++G+ I  D H++PASHVT+ND I+I QYI ST+TPMA ISSV  +L V P+P +A
Sbjct: 440 VNDEESGNEIKADPHIIPASHVTYNDSITISQYISSTRTPMAYISSVTAKLGVTPAPTIA 499

Query: 484 DFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPH 543
            FS RGPS IE SILKPDITAPGVNIIAAYPD IPL  L VDDRR PF V SGTSM+CPH
Sbjct: 500 RFSGRGPSIIEESILKPDITAPGVNIIAAYPDGIPLANLPVDDRRVPFMVYSGTSMSCPH 559

Query: 544 VAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNN 603
           V+GIVGLLK+  PKWSPAAIKSAIMTTAKT  +  +PI+D  G+ ATPLAYG GHV PN+
Sbjct: 560 VSGIVGLLKTLNPKWSPAAIKSAIMTTAKTRDSTLHPIVDLDGVIATPLAYGAGHVHPNS 619

Query: 604 VMDPGLVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQSFKVTDLNYPSISVTDIKT 663
            MDPGLVYDI+ID+YLNFLCARG NA QI + S+  FVC++SFKVTDLNYPSISV D+KT
Sbjct: 620 AMDPGLVYDITIDEYLNFLCARGYNATQIKQFSNNTFVCNRSFKVTDLNYPSISVADLKT 679

Query: 664 GPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQ 690
           GPVT+NRK+KNVGS G YVA+V +PLE SI VEPSTLQFTAMDEEKSF++VLQRSGKG+Q
Sbjct: 680 GPVTINRKVKNVGSPGKYVARVTSPLEASIVVEPSTLQFTAMDEEKSFRVVLQRSGKGNQ 739

BLAST of Cp4.1LG08g08230 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 703.4 bits (1814), Expect = 1.8e-202
Identity = 370/741 (49.93%), Postives = 477/741 (64.37%), Query Frame = 0

Query: 14  YIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSYNRHINGFAAMLD 73
           Y+VY G+ SH    +     RV E+HY  L S +GS+  A +AI +SY +HINGFAA LD
Sbjct: 32  YVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHLD 91

Query: 74  ENQASELA---------------------------------------------------- 133
            + A E++                                                    
Sbjct: 92  HDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIAN 151

Query: 134 --------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFFNKGY----GILN 193
                   +KSF DEG GPIPSRWKG CQ+  D  FHCN+KLIGAR+FNKGY    G LN
Sbjct: 152 LDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLN 211

Query: 194 ATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCW-PVEDR 253
           ++F+SPRD +GHG+HTLS A G+FV G ++F   NGTAKGGSPRAR+A+YKVCW PV+  
Sbjct: 212 SSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGN 271

Query: 254 QCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFG 313
           +C D + LAA+DAAI DG DVISVS+GGEP  F  D++++G+FHA +  IVVVCSAGN G
Sbjct: 272 ECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSG 331

Query: 314 PTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKALAFNKFYPLINAVD 373
           P  +TVSNV+PW +TVGAST+DR+F + +VLGN K  KG S SS AL   KFYP++ +V+
Sbjct: 332 PADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVN 391

Query: 374 AKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKD 433
           AKA NAS+ DA++C   SLDP K  GKI+VCLRG   RV KG  VA  G  GM+L N   
Sbjct: 392 AKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYV 451

Query: 434 NGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSR 493
            G+ +  D H+LPA+ +T  D  ++ +YI  TK P+A I+  +T+L + P+PVMA FSS+
Sbjct: 452 TGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSK 511

Query: 494 GPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIV 553
           GPS +   ILKPDITAPGV++IAAY   +     Q D RR  F   SGTSM+CPH++GI 
Sbjct: 512 GPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIA 571

Query: 554 GLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYGNGHVDPNNVMDPG 613
           GLLK+RYP WSPAAI+SAIMTTA    +   PI + T +KATP ++G GHV PN  ++PG
Sbjct: 572 GLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPG 631

Query: 614 LVYDISIDDYLNFLCARGENAIQINKLSHKLFVCDQ-SFKVTDLNYPSISVTDIKTGPVT 673
           LVYD+ I DYLNFLC+ G NA QI+  S   F C      + +LNYPSI+V ++ +  VT
Sbjct: 632 LVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVT 691

Query: 674 VNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYA 689
           V+R +KNVG    Y  +V  P  V +AV+P++L FT + E+K+FK++L +S     +GY 
Sbjct: 692 VSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV 751

BLAST of Cp4.1LG08g08230 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 682.9 bits (1761), Expect = 2.6e-196
Identity = 370/754 (49.07%), Postives = 478/754 (63.40%), Query Frame = 0

Query: 2   LFQTCIIATRKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSY 61
           LF +   A +K YIVYLGS +H    SS H   V  SH + L S  GS   A+EAI +SY
Sbjct: 30  LFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSY 89

Query: 62  NRHINGFAAMLDENQASELA---------------------------------------- 121
            RHINGFAA+LDEN+A+E+A                                        
Sbjct: 90  KRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWN 149

Query: 122 --------------------TKSFSDEGYGPIPSRWKGTCQSDSDPNFHCNKKLIGARFF 181
                               +KSFSDEGYG +P+RWKG C  D      CN+KLIGAR+F
Sbjct: 150 KAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKD----VPCNRKLIGARYF 209

Query: 182 NKGY----GI-LNATFNSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARI 241
           NKGY    G+  NA++ + RD +GHG+HTLS A GNFV GANVF + NGTA GGSP+AR+
Sbjct: 210 NKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARV 269

Query: 242 ASYKVCW-PVEDRQCLDPNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQ 301
           A+YKVCW PV+  +C D + LAA +AAI DGVDV+S S+GG+  +++ D +++G+FHAV+
Sbjct: 270 AAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVK 329

Query: 302 HGIVVVCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTSFSSKAL 361
           +G+ VVCSAGN GP   TVSNV+PWV+TVGAS++DR+F  FV L N +  KGTS  SK L
Sbjct: 330 NGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL-SKPL 389

Query: 362 AFNKFYPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQ 421
              K Y LI+A DA   N + +DA +C + SLDP K+ GKI+VCLRG  +RV KG   A 
Sbjct: 390 PEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAA 449

Query: 422 AGAAGMILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELD 481
           AGAAGM+L NDK +G+ I +D H+LPAS + + DG ++F Y+ STK P   I +    L+
Sbjct: 450 AGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLN 509

Query: 482 VNPSPVMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDS 541
             P+P MA FSSRGP+TI   ILKPDITAPGVNIIAA+ +     +L  D+RR PF  +S
Sbjct: 510 TKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTES 569

Query: 542 GTSMACPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILDFTGLKATPLAYG 601
           GTSM+CPH++G+VGLLK+ +P WSPAAI+SAIMTT++T  N   P++D +  KA P +YG
Sbjct: 570 GTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYG 629

Query: 602 NGHVDPNNVMDPGLVYDISIDDYLNFLCARGENAIQINKLSH-KLFVCDQSFKVTDLNYP 661
           +GHV PN    PGLVYD++  DYL+FLCA G N   +   +    + C Q   + D NYP
Sbjct: 630 SGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYP 689

Query: 662 SISVTDIKTGPVTVNRKLKNVGSLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIV 689
           SI+V ++ TG +TV RKLKNVG   TY A+ + PL V ++VEP  L F    E K F++ 
Sbjct: 690 SITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMT 749

BLAST of Cp4.1LG08g08230 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 519.6 bits (1337), Expect = 3.8e-147
Identity = 285/620 (45.97%), Postives = 392/620 (63.23%), Query Frame = 0

Query: 82  TKSFSDEGYGPIPSRWKGTCQSDSDPNFH---CNKKLIGARFFNKGY----GILNAT--F 141
           +KS+SDEG+GPIPS WKG C++ +  NF    CN+KLIGARFF +GY    G ++ +   
Sbjct: 146 SKSYSDEGFGPIPSSWKGGCEAGT--NFTASLCNRKLIGARFFARGYESTMGPIDESKES 205

Query: 142 NSPRDQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLD 201
            SPRD +GHGTHT S A G+ V GA++   A+GTA+G +PRAR+A YKVCW      C  
Sbjct: 206 RSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL---GGCFS 265

Query: 202 PNALAAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPA 261
            + LAA D AI+D V+V+S+S+GG   ++ RD +++GAF A++ GI+V CSAGN GP+ +
Sbjct: 266 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 325

Query: 262 TVSNVSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTS-FSSKALAFNKFYPLINAVDAKA 321
           ++SNV+PW+ TVGA T+DRDF    +LGN K   G S F  +AL  +K  P I A     
Sbjct: 326 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP-DKLLPFIYA----G 385

Query: 322 NNASSSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGD 381
           N +++++  +C   +L P K+ GKIV+C RG+ +RV KG VV  AG  GMIL N   NG+
Sbjct: 386 NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGE 445

Query: 382 AIATDLHLLPASHVTFNDGISIFQYIKSTKTPMACISSVKTELDVNPSPVMADFSSRGPS 441
            +  D HLLPA+ V    G  I  Y+ +   P A IS + T + V PSPV+A FSSRGP+
Sbjct: 446 ELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPN 505

Query: 442 TIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLL 501
           +I  +ILKPD+ APGVNI+AA+        L  D RR  F + SGTSM+CPHV+G+  LL
Sbjct: 506 SITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALL 565

Query: 502 KSRYPKWSPAAIKSAIMTTAKTNANNFNPILDF-TGLKATPLAYGNGHVDPNNVMDPGLV 561
           KS +P+WSPAAI+SA+MTTA     +  P+LD  TG  +TP  +G GHV P    +PGL+
Sbjct: 566 KSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLI 625

Query: 562 YDISIDDYLNFLCARGENAIQINKLSHKLFVCD--QSFKVTDLNYPSISVTDIKTGPVTV 621
           YD++ +DYL FLCA    + QI  +S + + CD  +S+ V DLNYPS +V     G    
Sbjct: 626 YDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKY 685

Query: 622 NRKLKNVGSLGTYVAQVKAPLE-VSIAVEPSTLQFTAMDEEKSFKIVLQRSGKGSQEGYA 681
            R + +VG  GTY  +V +    V I+VEP+ L F   +E+KS+ +             +
Sbjct: 686 TRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNS 745

Query: 682 FGELAWSDGKHNVRSSIAVN 688
           FG + WSDGKH V S +A++
Sbjct: 746 FGSIEWSDGKHVVGSPVAIS 755

BLAST of Cp4.1LG08g08230 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 484.6 bits (1246), Expect = 1.4e-136
Identity = 277/631 (43.90%), Postives = 381/631 (60.38%), Query Frame = 0

Query: 84  SFSDEGYGPIPSRWKGTCQSDSD-PNFHCNKKLIGARFFNKGY----GILNAT--FNSPR 143
           SF D G GP+P +WKG C +  D P   CN+KL+GARFF  GY    G +N T  F SPR
Sbjct: 146 SFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPR 205

Query: 144 DQEGHGTHTLSIAGGNFVSGANVFAMANGTAKGGSPRARIASYKVCWPVEDRQCLDPNAL 203
           D +GHGTHT SI+ G +V  A+    A+G A G +P+AR+A+YKVCW   +  C D + L
Sbjct: 206 DSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW---NSGCYDSDIL 265

Query: 204 AAYDAAISDGVDVISVSIGGEPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSN 263
           AA+D A++DGVDVIS+S+GG    +  DA+++GAF A+  GI V  SAGN GP   TV+N
Sbjct: 266 AAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTN 325

Query: 264 VSPWVLTVGASTIDRDFTNFVVLGNKKKLKGTS-FSSKALAFNKFYPLINAVDAKANNAS 323
           V+PW+ TVGA TIDRDF   V LGN K + G S +    L   + YPL+        +  
Sbjct: 326 VAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY 385

Query: 324 SSDAEVCNERSLDPTKLAGKIVVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIAT 383
           SS   +C E SLDP  + GKIV+C RG+ SR +KG +V + G  GMI+ N   +G+ +  
Sbjct: 386 SS--SLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVA 445

Query: 384 DLHLLPASHVTFNDGISIFQYI------KSTKTPMACISSVKTELDVNPSPVMADFSSRG 443
           D H+LPA+ V  + G  I +YI      +S+K P A I    T L + P+PV+A FS+RG
Sbjct: 446 DCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARG 505

Query: 444 PSTIEGSILKPDITAPGVNIIAAYPDEIPLIELQVDDRRAPFKVDSGTSMACPHVAGIVG 503
           P+     ILKPD+ APG+NI+AA+PD I    +  D+RR  F + SGTSMACPHV+G+  
Sbjct: 506 PNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAA 565

Query: 504 LLKSRYPKWSPAAIKSAIMTTAKTNANNFNPILD-FTGLKATPLAYGNGHVDPNNVMDPG 563
           LLK+ +P WSPAAI+SA++TTA T  N+  P++D  TG  ++ + YG+GHV P   MDPG
Sbjct: 566 LLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPG 625

Query: 564 LVYDISIDDYLNFLCARGENAIQINKLSHKLFVCD---QSFKVTDLNYPSISVTDIKTGP 623
           LVYDI+  DY+NFLC        I  ++ +   CD   ++  V +LNYPS SV   + G 
Sbjct: 626 LVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGE 685

Query: 624 VTVN----RKLKNVG-SLGTYVAQVKAPLEVSIAVEPSTLQFTAMDEEKSFKIVLQRSGK 683
             ++    R + NVG S   Y  +++ P   ++ VEP  L F  + ++ SF + ++ +  
Sbjct: 686 SKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEV 745

Query: 684 GSQEG---YAFGELAWSDGKHNVRSSIAVNL 689
               G      G + WSDGK NV S + V L
Sbjct: 746 KLSPGATNVETGHIVWSDGKRNVTSPLVVTL 771

BLAST of Cp4.1LG08g08230 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 482.3 bits (1240), Expect = 6.7e-136
Identity = 311/781 (39.82%), Postives = 421/781 (53.91%), Query Frame = 0

Query: 11  RKPYIVYLGSLSHGSSTSSLHHQRVTESHYSLLKSVSGSKNIAEEAISHSYNRHINGFAA 70
           ++ YIVY G         + H   + E H+S L+SV  S+  A  ++ +SY   INGFAA
Sbjct: 24  KQVYIVYFG---EHKGDKAFH--EIEEHHHSYLQSVKESEEDARASLLYSYKHSINGFAA 83

Query: 71  MLDENQASEL-------------------------------------------------- 130
            L  +QAS+L                                                  
Sbjct: 84  ELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRF 143

Query: 131 ---------------------------ATKSFSDEGYGPIPSRWKGTCQSDSDPN-FHCN 190
                                       +KSF+D+G GP+P  WKG CQ+    N  HCN
Sbjct: 144 RVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCN 203

Query: 191 KKLIGARFFNKG----YGILNATFN----SPRDQEGHGTHTLSIAGGNFVSGANVF-AMA 250
           +K+IGAR++ KG    YG  NAT N    SPRD +GHG+HT S A G  V GA+     A
Sbjct: 204 RKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFA 263

Query: 251 NGTAKGGSPRARIASYKVCW------PVEDRQCLDPNALAAYDAAISDGVDVISVSIG-G 310
            G+A GG+P AR+A YK CW       VE   CL+ + LAA D AI+DGV VIS+SIG  
Sbjct: 264 KGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTT 323

Query: 311 EPKEFLRDALSVGAFHAVQHGIVVVCSAGNFGPTPATVSNVSPWVLTVGASTIDRDFTNF 370
           EP  F +D +++GA HAV+  IVV  SAGN GP P T+SN++PW++TVGAST+DR F   
Sbjct: 324 EPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGG 383

Query: 371 VVLGNKKKLKGTSFSSKALAFNKFYPLINAVDAKANNASSSDAEVCNERSLDPTKLAGKI 430
           +VLGN   +K  S +  A   +KF PL+ A +      + ++   C   SL P  ++GK+
Sbjct: 384 LVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKV 443

Query: 431 VVCLRGVISRVSKGYVVAQAGAAGMILVNDKDNGDAIATDLHLLPASHVTFNDGISIFQY 490
           V+CLRG  SR+ KG  V +AG AGMIL N   NG+ + +D H +P + VT      I +Y
Sbjct: 444 VLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEY 503

Query: 491 IKSTKTPMACISSVKTELDVNPSPVMADFSSRGPSTIEGSILKPDITAPGVNIIAAYPDE 550
           IK+ K P A I   KT      +P M  FSSRGP+ ++ +ILKPDITAPG+ I+AA+   
Sbjct: 504 IKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGA 563

Query: 551 IPLIELQVDDRRAPFKVDSGTSMACPHVAGIVGLLKSRYPKWSPAAIKSAIMTTAKTNAN 610
               ++ VD R A + + SGTSM+CPHVAG + LLK+ +PKWS AAI+SA+MTTA    +
Sbjct: 564 DSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTND 623

Query: 611 NFNPILDFTGLKATPLAYGNGHVDPNNVMDPGLVYDISIDDYLNFLCARGENAIQINKLS 670
              PI D TGL A P A G+GH  P    DPGLVYD S   YL + C     ++ I  + 
Sbjct: 624 KKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGC-----SVNITNID 683

Query: 671 HKLFVCDQSFKV-TDLNYPSISVTDIKTGPVTVNRKLKNVG---SLGTYVAQVKAPLEVS 689
              F C        + NYPSI+V ++K   VTV R + NVG   S  TY+  VK P  +S
Sbjct: 684 -PTFKCPSKIPPGYNHNYPSIAVPNLKK-TVTVKRTVTNVGTGNSTSTYLFSVKPPSGIS 743