Cp4.1LG08g04320 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG08g04320 vs. ExPASy Swiss-Prot
Match: Q3UHX9 (Putative methyltransferase C9orf114 homolog OS=Mus musculus OX=10090 GN=Spout1 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.1e-04
Identity = 29/63 (46.03%), Postives = 43/63 (68.25%), Query Frame = 0

Query: 6   RKKKKKRKFENEIEGERRRSISKPRVI-IAVSGSIIDNAQSLETASRLAGQIARAATIFR 65
           ++++ KR+ E E    +R +  +P  + +A+ GSI+DNAQS E  + LAGQIARA TIF 
Sbjct: 50  QEEEAKRQEEEEEAAAQRSNQGRPYTLSVALPGSILDNAQSPELRTYLAGQIARACTIFC 109

Query: 66  IDE 68
           +DE
Sbjct: 110 VDE 112

BLAST of Cp4.1LG08g04320 vs. ExPASy Swiss-Prot
Match: Q10950 (Putative methyltransferase B0361.6 OS=Caenorhabditis elegans OX=6239 GN=B0361.6 PE=3 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.3e-04
Identity = 28/68 (41.18%), Postives = 41/68 (60.29%), Query Frame = 0

Query: 6   RKKKKKRKFENEIEGERRRSISKP------RVIIAVSGSIIDNAQSLETASRLAGQIARA 65
           +K+K + K   E E  ++  I K        + IAV G  ++NAQS E  + +AGQIARA
Sbjct: 37  KKRKNEEKIIKEAEEAKKAKIEKVDHTPPFTISIAVPGQFLNNAQSAELRTYMAGQIARA 96

Query: 66  ATIFRIDE 68
           AT++R+DE
Sbjct: 97  ATLYRVDE 104

BLAST of Cp4.1LG08g04320 vs. NCBI nr
Match: XP_022138246.1 (putative methyltransferase C9orf114 [Momordica charantia])

HSP 1 Score: 137 bits (345), Expect = 1.14e-35
Identity = 94/191 (49.21%), Postives = 100/191 (52.36%), Query Frame = 0

Query: 7   KKKKKRKFENE-IEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFRI 66
           K+KKK+K ENE IEGE+R  +SKP V IAVSGSIIDNAQSLE A+RLAGQIARAATIFRI
Sbjct: 35  KRKKKKKLENEEIEGEKREPVSKPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRI 94

Query: 67  DE---------------------------------------------------------- 126
           DE                                                          
Sbjct: 95  DEVVVFDSGRNSVTGSDTVAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRF 154

Query: 127 -GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARVTP 137
            GMLPPLDAPHHLRKHEWGPY E                    GVTLT +ERAPDAR TP
Sbjct: 155 VGMLPPLDAPHHLRKHEWGPYRE--------------------GVTLTQRERAPDARGTP 205

BLAST of Cp4.1LG08g04320 vs. NCBI nr
Match: KAF5941741.1 (hypothetical protein HYC85_019383 [Camellia sinensis])

HSP 1 Score: 135 bits (339), Expect = 3.62e-35
Identity = 81/130 (62.31%), Postives = 92/130 (70.77%), Query Frame = 0

Query: 8   KKKKRKFENEIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFRIDE 67
           KKKK+K ENE +  + + I  P V IAV GSIIDNAQSLE A+RLAGQIARAATIFRIDE
Sbjct: 42  KKKKKKRENEKDRVQPKEI--PTVSIAVPGSIIDNAQSLELATRLAGQIARAATIFRIDE 101

Query: 68  GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARVTPV 127
           G+LPPLDAPHHLRKHEW PY E  F  T S          +L   +TLKE+AP+   T V
Sbjct: 102 GLLPPLDAPHHLRKHEWAPYREGCFLLTFS----------ILVCCVTLKEQAPNFASTLV 159

Query: 128 DVGLSKAVYV 137
           DVGLSK V +
Sbjct: 162 DVGLSKNVLI 159

BLAST of Cp4.1LG08g04320 vs. NCBI nr
Match: XP_038879615.1 (putative methyltransferase C9orf114 [Benincasa hispida])

HSP 1 Score: 130 bits (327), Expect = 5.25e-33
Identity = 99/211 (46.92%), Postives = 107/211 (50.71%), Query Frame = 0

Query: 7   KKKKKRKFEN-EIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFRI 66
           KKKKK+K EN EI+ E+R+ ISKP V IAVSGSIIDNAQSLE A+RLAGQIARAATIFRI
Sbjct: 39  KKKKKKKVENDEIKVEKRKPISKPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRI 98

Query: 67  DE---------------------------------------------------------- 126
           DE                                                          
Sbjct: 99  DEVVVFDSGRSSMTGSDIAAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRF 158

Query: 127 -GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARVTP 157
            GMLPPLDAPHHLRKHEWGPY E                    GVTL  KERAPDAR TP
Sbjct: 159 VGMLPPLDAPHHLRKHEWGPYRE--------------------GVTL--KERAPDARGTP 218

BLAST of Cp4.1LG08g04320 vs. NCBI nr
Match: XP_028118657.1 (putative methyltransferase C9orf114 isoform X2 [Camellia sinensis])

HSP 1 Score: 127 bits (320), Expect = 1.84e-32
Identity = 80/130 (61.54%), Postives = 88/130 (67.69%), Query Frame = 0

Query: 8   KKKKRKFENEIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFRIDE 67
           KKKKRK ENE +  + + I  P V IAV GSIIDNAQSLE A+RLAGQIARAATIFRIDE
Sbjct: 42  KKKKRKRENEKDHVQPKEI--PTVSIAVPGSIIDNAQSLELATRLAGQIARAATIFRIDE 101

Query: 68  GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARVTPV 127
           G+LPPLDAPHHLRKHEW PY E                    GVTL  KE+AP+   T V
Sbjct: 102 GLLPPLDAPHHLRKHEWAPYRE--------------------GVTL--KEQAPNFASTLV 147

Query: 128 DVGLSKAVYV 137
           DVGLSK V +
Sbjct: 162 DVGLSKNVLI 147

BLAST of Cp4.1LG08g04320 vs. NCBI nr
Match: XP_040973743.1 (putative methyltransferase C9orf114 homolog isoform X2 [Gossypium hirsutum] >KAB2095269.1 hypothetical protein ES319_A01G024700v1 [Gossypium barbadense])

HSP 1 Score: 127 bits (319), Expect = 2.43e-32
Identity = 76/133 (57.14%), Postives = 86/133 (64.66%), Query Frame = 0

Query: 5   HRKKKKKRKFENEIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFR 64
           HRKKK K K E E  GE ++S   P V +A++GSI+DNAQSLE A+RLA QIARAATIFR
Sbjct: 36  HRKKKPKTKPEKETNGETKKSDDTPTVTVAIAGSIVDNAQSLELATRLASQIARAATIFR 95

Query: 65  IDEGMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARV 124
           IDEGMLPPLDAPHHLRKHEW PY E                    GVTL  +E+   +  
Sbjct: 96  IDEGMLPPLDAPHHLRKHEWAPYRE--------------------GVTL--EEKPTSSMG 146

Query: 125 TPVDVGLSKAVYV 137
           T VDVGL K V V
Sbjct: 156 TVVDVGLDKNVVV 146

BLAST of Cp4.1LG08g04320 vs. ExPASy TrEMBL
Match: A0A6J1C971 (putative methyltransferase C9orf114 OS=Momordica charantia OX=3673 GN=LOC111009468 PE=3 SV=1)

HSP 1 Score: 137 bits (345), Expect = 5.51e-36
Identity = 94/191 (49.21%), Postives = 100/191 (52.36%), Query Frame = 0

Query: 7   KKKKKRKFENE-IEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFRI 66
           K+KKK+K ENE IEGE+R  +SKP V IAVSGSIIDNAQSLE A+RLAGQIARAATIFRI
Sbjct: 35  KRKKKKKLENEEIEGEKREPVSKPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRI 94

Query: 67  DE---------------------------------------------------------- 126
           DE                                                          
Sbjct: 95  DEVVVFDSGRNSVTGSDTVAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRF 154

Query: 127 -GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARVTP 137
            GMLPPLDAPHHLRKHEWGPY E                    GVTLT +ERAPDAR TP
Sbjct: 155 VGMLPPLDAPHHLRKHEWGPYRE--------------------GVTLTQRERAPDARGTP 205

BLAST of Cp4.1LG08g04320 vs. ExPASy TrEMBL
Match: A0A7J7GP98 (Uncharacterized protein OS=Camellia sinensis OX=4442 GN=HYC85_019383 PE=3 SV=1)

HSP 1 Score: 135 bits (339), Expect = 1.75e-35
Identity = 81/130 (62.31%), Postives = 92/130 (70.77%), Query Frame = 0

Query: 8   KKKKRKFENEIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFRIDE 67
           KKKK+K ENE +  + + I  P V IAV GSIIDNAQSLE A+RLAGQIARAATIFRIDE
Sbjct: 42  KKKKKKRENEKDRVQPKEI--PTVSIAVPGSIIDNAQSLELATRLAGQIARAATIFRIDE 101

Query: 68  GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARVTPV 127
           G+LPPLDAPHHLRKHEW PY E  F  T S          +L   +TLKE+AP+   T V
Sbjct: 102 GLLPPLDAPHHLRKHEWAPYREGCFLLTFS----------ILVCCVTLKEQAPNFASTLV 159

Query: 128 DVGLSKAVYV 137
           DVGLSK V +
Sbjct: 162 DVGLSKNVLI 159

BLAST of Cp4.1LG08g04320 vs. ExPASy TrEMBL
Match: A0A6P4Q7Q5 (putative methyltransferase C9orf114 homolog isoform X2 OS=Gossypium arboreum OX=29729 GN=LOC108480795 PE=3 SV=1)

HSP 1 Score: 127 bits (319), Expect = 1.18e-32
Identity = 76/133 (57.14%), Postives = 86/133 (64.66%), Query Frame = 0

Query: 5   HRKKKKKRKFENEIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFR 64
           HRKKK K K E E  GE ++S   P V +A++GSI+DNAQSLE A+RLA QIARAATIFR
Sbjct: 36  HRKKKPKTKPEKETNGETKKSDDTPTVTVAIAGSIVDNAQSLELATRLASQIARAATIFR 95

Query: 65  IDEGMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARV 124
           IDEGMLPPLDAPHHLRKHEW PY E                    GVTL  +E+   +  
Sbjct: 96  IDEGMLPPLDAPHHLRKHEWAPYRE--------------------GVTL--EEKPTSSMG 146

Query: 125 TPVDVGLSKAVYV 137
           T VDVGL K V V
Sbjct: 156 TVVDVGLDKNVVV 146

BLAST of Cp4.1LG08g04320 vs. ExPASy TrEMBL
Match: A0A5J5WVC8 (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=ES319_A01G024700v1 PE=3 SV=1)

HSP 1 Score: 127 bits (319), Expect = 1.18e-32
Identity = 76/133 (57.14%), Postives = 86/133 (64.66%), Query Frame = 0

Query: 5   HRKKKKKRKFENEIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATIFR 64
           HRKKK K K E E  GE ++S   P V +A++GSI+DNAQSLE A+RLA QIARAATIFR
Sbjct: 36  HRKKKPKTKPEKETNGETKKSDDTPTVTVAIAGSIVDNAQSLELATRLASQIARAATIFR 95

Query: 65  IDEGMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDARV 124
           IDEGMLPPLDAPHHLRKHEW PY E                    GVTL  +E+   +  
Sbjct: 96  IDEGMLPPLDAPHHLRKHEWAPYRE--------------------GVTL--EEKPTSSMG 146

Query: 125 TPVDVGLSKAVYV 137
           T VDVGL K V V
Sbjct: 156 TVVDVGLDKNVVV 146

BLAST of Cp4.1LG08g04320 vs. ExPASy TrEMBL
Match: A0A6J1GWH0 (putative methyltransferase C9orf114 homolog OS=Cucurbita moschata OX=3662 GN=LOC111458056 PE=3 SV=1)

HSP 1 Score: 127 bits (320), Expect = 2.85e-32
Identity = 94/194 (48.45%), Postives = 99/194 (51.03%), Query Frame = 0

Query: 7   KKKKKRKFEN----EIEGERRRSISKPRVIIAVSGSIIDNAQSLETASRLAGQIARAATI 66
           KKKKKRK +N    EIEGE+R+ ISKP V +AVSGSIIDNAQSLE A+RLAGQIARAATI
Sbjct: 39  KKKKKRKKQNVENEEIEGEKRKPISKPTVSMAVSGSIIDNAQSLELATRLAGQIARAATI 98

Query: 67  FRIDE------------------------------------------------------- 126
           FRIDE                                                       
Sbjct: 99  FRIDEVVVFDSGRSSMTGSDVAAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKHNN 158

Query: 127 ----GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKERAPDAR 137
               GMLPPLDAPHHLRKHEWGPY E                    GVTL  KERAPDAR
Sbjct: 159 LRFVGMLPPLDAPHHLRKHEWGPYRE--------------------GVTL--KERAPDAR 210

BLAST of Cp4.1LG08g04320 vs. TAIR 10
Match: AT5G19300.1 (CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Protein of unknown function DUF171 (InterPro:IPR003750); Has 3649 Blast hits to 1964 proteins in 291 species: Archae - 113; Bacteria - 121; Metazoa - 1082; Fungi - 399; Plants - 227; Viruses - 4; Other Eukaryotes - 1703 (source: NCBI BLink). )

HSP 1 Score: 88.6 bits (218), Expect = 5.1e-18
Identity = 74/200 (37.00%), Postives = 90/200 (45.00%), Query Frame = 0

Query: 7   KKKKKRKFENE----------IEGERRRSISKPR--VIIAVSGSIIDNAQSLETASRLAG 66
           KKK+K K + E          ++ E + ++ + R  V IA++GSII N QSLE A+RLAG
Sbjct: 64  KKKRKNKNQEEEPELVTEKTKVQEEEKGNVEEGRATVSIAIAGSIIHNTQSLELATRLAG 123

Query: 67  QIARAATIFRIDE----------------------------------------------- 126
           QIARAATIFRIDE                                               
Sbjct: 124 QIARAATIFRIDEIVVFDNKSSSEIESAATNASDSNESGASFLVRILKYLETPQYLRKSL 183

Query: 127 ----------GMLPPLDAPHHLRKHEWGPYIEKSFPATISFVHLFLLQIFLLGVTLTLKE 138
                     GMLPPLDAPHHLRKHEW  Y E                    GVTL+  E
Sbjct: 184 FPKQNDLRYVGMLPPLDAPHHLRKHEWEQYRE--------------------GVTLS--E 241