Cp4.1LG07g09060 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g09060
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionkunitz trypsin inhibitor 1-like
LocationCp4.1LG07: 8216104 .. 8216744 (+)
RNA-Seq ExpressionCp4.1LG07g09060
SyntenyCp4.1LG07g09060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCTGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACCGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTACCACTTCGTTCACAAGAAATAGGTATTATTGCATCACTATCGAACTCAAAAACTCAATTTGATCAGTGATACCATTAGAAAACTCTTGATTATATTACGAATTCTTAACCATAGGTTTTGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCGTTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACAATGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCATTTATAATATAGGATGGTGTCCGTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

mRNA sequence

ATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCTGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACCGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTACCACTTCGTTCACAAGAAATAGGTTTTGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCGTTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACAATGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCATTTATAATATAGGATGGTGTCCGTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Coding sequence (CDS)

ATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCTGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCCATCACCGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTACCACTTCGTTCACAAGAAATAGGTTTTGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTTGTGTTTCAAGCGTTTTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACAATGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCATTTATAATATAGGATGGTGTCCGTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Protein sequence

MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFVRA
Homology
[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 3.7e-38
Identity = 89/186 (47.85%), Postives = 119/186 (63.98%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           +  +SIAQ   V+DT G P++ + +YFI+PAIT   G+LTL+TR  + CPF+VG  P   
Sbjct: 18  VVTTSIAQF--VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNVGLDPDAP 77

Query: 61  QEIGFGVTFTPYLPG--EDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 120
           Q  GF V  +P++    ED +R GRDL+V+FQA + C  ST WR+GE D TTGRRF+ TG
Sbjct: 78  Q--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITG 137

Query: 121 DNDTATG----YFRI-DRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGS 180
            +D+  G    +FRI    + GI+NI WCP++V    +  CG  GI+ E G  LLALDGS
Sbjct: 138 RDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGS 197

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.6e-33
Identity = 82/185 (44.32%), Postives = 107/185 (57.84%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MA +SIAQ   V+DT G P++ + EYFI+PAIT   G  TL+T  G  CP HVG +    
Sbjct: 18  MATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-PCPLHVG-LDNTE 77

Query: 61  QEIGFGVTFTPYLP--GEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 120
             +G  V FTP+ P   +D +R  RDL+V F   + C  ST WR+GE D T+GRR + TG
Sbjct: 78  GTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDATSGRRLIVTG 137

Query: 121 -DNDTAT--GYFRI-DRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSA 180
            DN   +   +FRI      G YNI WCP++     + +CG  G++ E G  LLALDG A
Sbjct: 138 RDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENGKNLLALDGDA 197

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 5.2e-32
Identity = 78/186 (41.94%), Postives = 107/186 (57.53%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           +  ++IAQ   VLDT G P++ + EYFI+P IT+  G  T+++R  + CP HVG + L  
Sbjct: 20  ITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-NESCPLHVG-LELTG 79

Query: 61  QEIGFGVTFTPYLPGE--DTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 120
              G  V FTP+ P    D +R  RDL++ FQA S C+ ST WR+GE D  +GRR + TG
Sbjct: 80  LGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEKDTKSGRRLIITG 139

Query: 121 DNDTATG----YFRI-DRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGS 180
            +    G    +FRI +    G+YNI WCP++V    +  CG   +L E G  LLALDG 
Sbjct: 140 TDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLNENGKILLALDGG 199

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.5e-15
Identity = 56/179 (31.28%), Postives = 81/179 (45.25%), Query Frame = 0 

Query: 12  VLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTFTP 71
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V F+ 
Sbjct: 30  VVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 72  YLPGEDTIREGRDLKVVFQ-AFSICITSTAWRVGETDPTTGRRFVETG------DNDTAT 131
           +      + E ++L +      +ICI ST WRVGE D    + FV  G        D+  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 132 GYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRLLALDGSAFPFEFVRA 183
            +F+I++     Y   +CP      G P+C   GI I E G+R LAL    F   F +A
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKA 205

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 1.7e-14
Identity = 43/117 (36.75%), Postives = 61/117 (52.14%), Query Frame = 0 

Query: 4   SSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEI 63
           S  A  PPV DT+G  L+ +  Y++  A     G LT+    G  CP  V Q P   Q  
Sbjct: 20  SRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHD 79

Query: 64  GFGVTFTPY--LPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 119
           GF V  TPY   P +  IR   D+++ F+A++ C+ ST W + +++   GRR V TG
Sbjct: 80  GFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAAGRRHVITG 134

[Close]
BLAST of Cp4.1LG07g09060 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380 bits (976), Expect = 4.90e-133
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 17  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 76

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
           QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN
Sbjct: 77  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 136

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 137 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 196

Query: 181 RA 182
           RA
Sbjct: 197 RA 198

[Close]
BLAST of Cp4.1LG07g09060 vs. NCBI nr
Match: KAG6586275.1 (hypothetical protein SDJN03_19008, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 377 bits (969), Expect = 3.24e-132
Identity = 179/182 (98.35%), Postives = 182/182 (100.00%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MAVSSIAQSPPVLDTEGRPLQINVEYFIKPA+TDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 60

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
           QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGD+
Sbjct: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 120

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 121 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180

Query: 181 RA 182
           RA
Sbjct: 181 RA 182

[Close]
BLAST of Cp4.1LG07g09060 vs. NCBI nr
Match: KAG7021121.1 (hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377 bits (969), Expect = 5.52e-132
Identity = 179/182 (98.35%), Postives = 182/182 (100.00%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MAVSSIAQSPPVLDTEGRPLQINVEYFIKPA+TDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 16  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 75

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
           QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGD+
Sbjct: 76  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 135

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 136 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 195

Query: 181 RA 182
           RA
Sbjct: 196 RA 197

[Close]
BLAST of Cp4.1LG07g09060 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 377 bits (969), Expect = 5.72e-132
Identity = 179/182 (98.35%), Postives = 182/182 (100.00%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MAVSSIAQSPPVLDTEGRPLQINVEYFIKPA+TDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 17  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 76

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
           QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGD+
Sbjct: 77  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 136

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 137 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 196

Query: 181 RA 182
           RA
Sbjct: 197 RA 198

[Close]
BLAST of Cp4.1LG07g09060 vs. NCBI nr
Match: XP_022965952.1 (kunitz trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 366 bits (940), Expect = 1.51e-127
Identity = 174/182 (95.60%), Postives = 177/182 (97.25%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MA SSIAQSPPVLD EGRPL+INVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 17  MAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 76

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
           QEIGFGVTFTPYLP ED IREGRDLKVVFQAFSICITSTAWRVGE DPTTGRRFVETGD+
Sbjct: 77  QEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETGDD 136

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 137 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 196

Query: 181 RA 182
           RA
Sbjct: 197 RA 198

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 377 bits (969), Expect = 2.77e-132
Identity = 179/182 (98.35%), Postives = 182/182 (100.00%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MAVSSIAQSPPVLDTEGRPLQINVEYFIKPA+TDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 17  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 76

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
           QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGD+
Sbjct: 77  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 136

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 137 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 196

Query: 181 RA 182
           RA
Sbjct: 197 RA 198

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 366 bits (940), Expect = 7.32e-128
Identity = 174/182 (95.60%), Postives = 177/182 (97.25%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MA SSIAQSPPVLD EGRPL+INVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 17  MAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 76

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
           QEIGFGVTFTPYLP ED IREGRDLKVVFQAFSICITSTAWRVGE DPTTGRRFVETGD+
Sbjct: 77  QEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGEADPTTGRRFVETGDD 136

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 137 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 196

Query: 181 RA 182
           RA
Sbjct: 197 RA 198

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy TrEMBL
Match: A0A6J1DBS1 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1)

HSP 1 Score: 320 bits (820), Expect = 1.54e-109
Identity = 149/182 (81.87%), Postives = 166/182 (91.21%), Query Frame = 0 

Query: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRS 60
           MA+ S AQSPPVLDT+GRPLQ  VEYFIKPA+TDVAGNLTLLTRKGD CPFHVGQVPLRS
Sbjct: 19  MAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRKGDPCPFHVGQVPLRS 78

Query: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDN 120
            +IG+ +    +L GEDTIREGRDLKVVFQAFSICITSTAWRVGET+PTTGRR V TG++
Sbjct: 79  TDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWRVGETEPTTGRRLVATGND 138

Query: 121 DTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180
           DTATG+FRID+ + G+YN+GWCPSDVPIRGRPRCGRAGIL+EKG+RLLALDGSAFPFEFV
Sbjct: 139 DTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGVRLLALDGSAFPFEFV 198

Query: 181 RA 182
           RA
Sbjct: 199 RA 200

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 311 bits (798), Expect = 6.04e-106
Identity = 146/183 (79.78%), Postives = 162/183 (88.52%), Query Frame = 0 

Query: 1   MAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPL 60
           MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPAITDVAGNLTL+TRKGDQCPF+VGQVPL
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  RSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG 120
            SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE DP +GRRFV+ G
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 DNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFE 180
           +++TATGYFRID+ N G+YNIGWCPSDVP +GRPRCG AGILIEKG+R LALDG AFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

[Close]
BLAST of Cp4.1LG07g09060 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 299 bits (766), Expect = 4.67e-101
Identity = 141/184 (76.63%), Postives = 156/184 (84.78%), Query Frame = 0 

Query: 1   MAVSSIA---QSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVP 60
           MA+ S A     PPVLDTEGRPLQ + EYFIKPAITDVAGNLTL+TRKGDQCPF+VGQVP
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  LRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVET 120
           L SQE GFGV+  PY  GEDTIREGRDLK VFQ F+ICIT T W+VGE DP TGRRFV+ 
Sbjct: 79  LFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPF 180
           G ++TATGYFRID+ + G+YNIGWCPSDVPI+GRPRCG AGILIEKG+R LALDG AFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

[Close]
BLAST of Cp4.1LG07g09060 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 82.0 bits (201), Expect = 5.3e-16
Identity = 56/179 (31.28%), Postives = 81/179 (45.25%), Query Frame = 0 

Query: 12  VLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTFTP 71
           V+D +G  +  +  Y++ P I    G LTL  R G  CP+ + Q      E G  V F+ 
Sbjct: 30  VVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 72  YLPGEDTIREGRDLKVVFQ-AFSICITSTAWRVGETDPTTGRRFVETG------DNDTAT 131
           +      + E ++L +      +ICI ST WRVGE D    + FV  G        D+  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 132 GYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRLLALDGSAFPFEFVRA 183
            +F+I++     Y   +CP      G P+C   GI I E G+R LAL    F   F +A
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKA 205

[Close]
BLAST of Cp4.1LG07g09060 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 45.8 bits (107), Expect = 4.2e-05
Identity = 38/140 (27.14%), Postives = 56/140 (40.00%), Query Frame = 0 

Query: 9   SPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRK---GDQCPFHVGQVPLRSQEI-G 68
           S  VLD  G P+Q NV+Y+I PA     G L    R     D C  ++  V   S  + G
Sbjct: 29  SQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLC-LNLDIVQSSSPFVSG 88

Query: 69  FGVTFTPYLPGEDTIREGRDLKVVFQA-FSICITSTAWRVGETDPTTGRRFVETGDNDTA 128
             VTF+P       ++    L + F +   +C  S  WR+  +           G     
Sbjct: 89  LPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHSVQLRKSFVSIGGQKGKG 148

Query: 129 TGYFRIDRDNFGIYNIGWCP 144
             +F+I  D    Y + +CP
Sbjct: 149 NSWFQIQEDG-DAYKLMYCP 166

The following BLAST results are available for this feature:
BLAST of Cp4.1LG07g09060 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
G7LCV73.7e-3847.85Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ... [more]
A0A072TH681.6e-3344.32Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ... [more]
G7LCV15.2e-3241.94Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ... [more]
Q8RXD57.5e-1531.28Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1[more]
P075961.7e-1436.75Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2[more]
BLAST of Cp4.1LG07g09060 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023521354.14.90e-133100.00alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381... [more]
KAG6586275.13.24e-13298.35hypothetical protein SDJN03_19008, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7021121.15.52e-13298.35hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022938134.15.72e-13298.35alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata][more]
XP_022965952.11.51e-12795.60kunitz trypsin inhibitor 1-like [Cucurbita maxima][more]
BLAST of Cp4.1LG07g09060 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FD702.77e-13298.35alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1HLP67.32e-12895.60kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3... [more]
A0A6J1DBS11.54e-10981.87miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1[more]
A0A0A0LGB46.04e-10679.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1[more]
A0A5A7V7Y24.67e-10176.63Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O... [more]
BLAST of Cp4.1LG07g09060 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G73260.15.3e-1631.28kunitz trypsin inhibitor 1 [more]
AT1G73325.14.2e-0527.14Kunitz family trypsin and protease inhibitor protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 11..182
e-value: 1.2E-21
score: 88.0
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 12..180
e-value: 4.7E-37
score: 127.6
IPR002160Proteinase inhibitor I3, Kunitz legumePANTHERPTHR33107KUNITZ TRYPSIN INHIBITOR 2coord: 1..182
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 12..28
NoneNo IPR availableGENE3D2.80.10.50coord: 8..182
e-value: 1.4E-39
score: 137.6
NoneNo IPR availablePANTHERPTHR33107:SF51PUTATIVE-RELATEDcoord: 1..182
IPR011065Kunitz inhibitor STI-like superfamilySUPERFAMILY50386STI-likecoord: 10..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g09060.1Cp4.1LG07g09060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
molecular_function GO:0004866 endopeptidase inhibitor activity