Cp4.1LG07g06420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG07g06420
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	mitotic checkpoint protein BUB3.3
Location	Cp4.1LG07: 4620074 .. 4625269 (-)
RNA-Seq Expression	Cp4.1LG07g06420
Synteny	Cp4.1LG07g06420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACGTGAGCCATTCACACAGCCAAGGATCATTTTTTCAAAAACCCTTCATTTGTTATTATACAGCTTGAAAGAGCGTCTCATCCAACTCAAAATTTGTTCTATTACTTCATTTCCCGCCTCTTTTGTTCGTCTAATCTCGATCCCCAGGCATGGAACCCAACAGGGTTTTGCTGGATTTTCAAAATCCGATCCAAGATGCCATCTCCACAATCCAATTCGCTCCCCGTTCGAACAATCTCCTTATTTCTTCCTGGGATTCTGTTAGTCTTCCTTTTGGTGTTCCAAATTCTTTCAACGTTTCTGCCATTTCTTTTCGTTCACTATGAATGAAGTTCTAGGTTTTCGTTTTTTTTTGGACAGAGTCTTCGTTTGTACGATGTGGATAATTCTGCACTCAGACTTGAAGCTCCCTCCGAAGCTGCACTGCTTGACTGCTGCTTCCAGGACGAGTCACTGGCTTTGAGCGCTGGTTCTGATGGCTTAATAAGAAGGTTTGGGGTTCGAATTTAATAATAAATCCTCTGGATTAATGTTATGAAATGAAATATCATGGTACAGATTAAGATATTTGGATCTAGCAATTATCTCGATTTGTTCCATTGTTCTTCTTTCAATTCATCATACTTGGAGAAATTGTTTGTCTATGGCTGTAACTATTAAGTTATTGAAATGATTTCCACAAAGTTGGGAAGGAAATTGTCTCTTTCAACCCCCTTCCACCAAATAGGAAAAGTCTCTGAAGTAAAACATGAGGATTATTTAGATGTTAATGATGGAGTTAAATTCTGTCCTGTTTTCTGCTGTGAAATAGTAAATGCAGTAGTTTGTTTGTTCTTCTTTTCATCTCATTCTGTTCTAGATAGTCAATTTTTTTGTCGGTTGATTTCTTAAGAGGATTATTAAAAGAACTACTTCAATTATGCATGAAAACCCCTCTTATTCTAGGTGAAGCCCCACGACAGTCATAAGAATACATGAATTTAAAAGGTACATATTGTAGTCAAGATAGGAATGATTCTGAGTTTTTAGATTCATTAAGTCTCTTGGATGAATAGTTTCCTTATCGAACTTGAATGGTTTCCCCTGATGTTCGATACCTCGAATCACATGTCGTTACAGTTTGGTATCAGAGCTAGACACCAAGTGGTATGCCAACGAAAATGCTGGGCCCTAAGGGGTTGGATGGAGGGGGGAATGAAACATTCAAGAATGGTGTGGACACTTCTCCCTAACAGATGCGTCTTAAAACTTCGAGGCGAAGCCCTTGAAGGGAAAACCCAAAGAAGACAATATCTGCTAGAGGTGGGCTTGGACTATTACAAATAGTATCAGAGCCAGTCACTGAACGGTGTGAGACACTGGTCGGAGTAGGGCTAGACTTTCTCCATAGTAGACGCGTTTTAAAACTGTGAGGTTGATGAAGATACATAACGGGCGAAAGCAGACAATATCTGTTAGCGGTGGGATTGGGTTGTTACAAATGGTATCAGAATCAAACACTGAGTGGTGTTCTAGCGAGGACGTTGGGCCCCAAAGAGGGATGGATTGTGAGATCCCACATCGGGTGGAGAGGAGAACGAAACATTCCTTATACGGGTGTGGAAACCTCTCCTTAACTGATGTGTTTTAAAACCTTGAGAGGAAGTTCTTGAAGAGAAAGCCCAAAGAGAGCAATATTTGCTAGCGGTAGGCTTGGGCTGTTATGTACTTCTTGTCATCTTTTAACTTTAATGGTTGGGCTGTGTGGCATAATGATTGACTCATTATTGGTTTGAATTTGAGAATAAGTTCTATTTGATCTTGGTTGAGCTAGTTGATCACTTATTTTCTTATCATTCTCCCTTTCTTCGAGAAGATTCGTCTTCGAATCTACAAGATCTCCTAGCAAAATAACTTTTTAGTCATCATCTGAATCTTGTTAAGCAAGTTTGAAAGAATCTTCTCTTCGAAACTCAAATGTGTAATTTCTTTGTAAAGGACTCCTCCCCTTTCGACATTGATCCAAAATTTCACAATTTATCATCCCATGTGTCTTGAACGAACCACCCAAAACACTTGGAAGAAAGCGTTTCTGGGATTACAAATCCCACTCAATGAGTTTTTCTCACATCCACTCTTTTCTTGTTCATAATGGAAGAAATGTCCCTTGGATAAGCCCATTTGTAATATTGTATCCGTAGTTTTTTTATGAGAAACAAAAAACGAATATAAACAAATGTATTGTATTATGAGTCTAACAAAAACAGACAAATTACAAGTATTTTAGTCTGTTACTTTATCTTGCAATGGTTTCTAAAAACGTTATTATAAATTTTTCCTTGGTTCTTTTCTTTACCTTTCTAGGTCTGTTTTATGCTAGCGGCATTGAGTCATGGTTGGATATTTTGACCATAGGTTGCTAATGGCTTTCATTAAAACTTTACAGGTATGACTTGCAATCAGGAATTTCTGAAACCATTGGAAAACATGATGATATTGCAACCAATATCAGATATTCGGATCAAACATGTGAGCTACTTTACCTCATGACATAATTTACGCGATTCATACACATATCAGTTACTTTTTGATAAATTATTTAAAATGGTGGAAACTATAATTTTTCCTTTTAGTATGCTTGTTAATAACTGGCTAATATTTTCCTTTTGAACTTGATGAACCGTGATTCTCATTGAGCCCTGTTCACTCAAGAATTGGCCAACTCTATGTCCATTGCAGTATTTCGTTAGCTATTATTGACCCTTTGTCACATTGGTTTTGAAAAGATAGAATTTACTCCCTTTATTTTTGTAGGCCAAATAGTAACAGCGGGTTTGGATGGAAAGATACTGTTGTGGGACACGCGTAGAGAAAAATGTGTGTCATTTGTGAGAAACAGGGGTTCAGATGTGGTGTCCATGTCTATCTCTGGATTTAATTTAATTGTGGCTTCTGGAATATGTGTATATTCACTGGACTTACGTAACATACAAAAACCAGTTCAACTCACAGATTCTTGTATGAAAGTTCCAGTAGCTTGTGTAAGCTCTGTTCCTTATACTGAAGGTTGGTTGACATATTATGCAGTTTCTTCCAGATAACTTGTAGCTAATATTCTGATTGTTGAAGGACGCCTATAAATCATCAGGACAACTTTTGCTTGAATTGTGTTCACTTCTAAATACTTTCTTTGCAGGATTTGCGATTGGCTCAGTAGATGGACGTGTAGCTTTGGAGATTGCTTGTCTGGACCAAGCAGATGACATCAGGTTTTTGCTTTCCCTACCCTTGGATATGGATGCTAATTACTTAAATCATATATTGAACTAGGTCGAGTTAGTATATTGCTTAAATGCTCTATCTTATTTGTAACGGTTCATGCCCACCACTAGCAGATATTGTCATCTTTGGACTTTTCCTTTCAGACTTCCCTTTAAGGTTTTTAAAATGCGTCTGTTAGGGAGAGGTTTTCACACTCTTATAAGGAGTGTTTCGTTCTCCTCCCCAACCGATGTGAGATCTCACATTATCCATCTAGGTACTACAGTCAATAATCTGTTGTTGGGTTCAGTTGCTTTTAGTCCATTATGGGAACACAAAAAACTTAGAACTTGTTTGGAGGTATTTTTACTGGGAATTGTTTACAAAAGACTGCTTAGCGACTTTGAAGACCTATTTGAAGTGTTGTCACCGATTTTCGTTCACTGATAACAATTTATGTTACGAACTTGTCTAACTTGTCTAATGTCATTGGAGTACCAGTGTACGTGTACTAATTGATGGTTATTATCTATACGAATGATGTGCACCTTCCTTTCTTGTGCCTTAAGCATAGAAAATCTAAAGTTATGTGCACCTTCCTTTCTTGTGCCTTAAGCATAGAAAATCAAAAGTTAAGTGTGCTTGGTTTAATCTTATATTGGGTGATCTTTTGTGAAATTTACGAGGAAGCAGGTGAGTTATGGTAGCATATTGATAGGACCCATGTGAAAATAGTCTTCAATCTAACAAGTAGCTTTAGACAAGTAAGGCGACATAGTCGATCGTAGGGAAATGCTAGAGCCGTCATGTGTTGAATCTAGATTCTGGATCTTAAGCTTAGGGTACTCTAGATTAAGACAAGTAAAGTGATGTAGTCAGGTCGCTGGAGAATGCTAGAATCGTCAAGGGTCAAACTTAGATTCCAAATCATAGGGCGTTCTCCATCCAATTACACTTAAGGCTTAATTAAGGATAAAGAGACATGTGAAAAGATTCATTTTCTGCCACGTTCCATCTTTCTAGAGTTTTTCAAGTGCTAAAGAAAGATATTCATGATATTCTTGAATCATTATTTGGCCTTGTCCAATAACTCCTGGGATGCATTATTCTTACACAGTATTTCCTTTATATTTTTGAAACAGATATATTTTCTGGTGTCACCCAAAGTCAAAGGGTAGAAGAAAGCATTTGGAGTCGGTGAATGATATTGTGTTTAATCCAATGTATGCATTATAGTTTCACCCAAAGTCAACATATTTTGTCACATTATGCTAATTTTGTATGTTGCATTTAGACATCATGGTGCCTTTGCAACGGGAGACAATGCCGGCTTTGTCAGCGTATGGGATTTCCAAAGCAAGAGAAGAATACTCGAGGTATGAGATTCTTTTTGTAATACTCTAAGTCCAATACTAGCATATATTGCTCTCTTTGAGCTTTTCCTTTAGACTTTAAAACGCGTCTGCTACCTTATAAATAGTGTTTCGTTCTCTTCTCCAATCAATGTGGGACTCTCACACTTTGCATTGTCCTCTAAAATTTATGTGATTAGGGTCGGCTATGTTTGATAATTCGTTCATTTGGTTTTAGTTGCCTAGATTTCCAAATAGCGTGGCATCGTTGTCGTACAACTGCGGAGGTCAACTTTTGGCTGTTGCTTCAAGTTGTACGTATCAAGAAGCTAAAGAAAGGTTCATTTTCTATTCATAATGTTTCTTCTCTTAAGCATTGCTTTATGTTTGGAGTTGATTTGGTGTTGTCATATATTATGTTTATGCAACAGAGAAGATGCTCCTCAAGTGTTCATACATGTAATGGAGGAGTTCAATCCCAAATCTTTCTCTGCCTCTTCCACTGGATAGCTTCACCTTTCCTACCATGCCTTGCTTCCCACTAAAGGTAATAAATTATTAGATTTTATGTCAAATTTATAGATGGAATATCCGAAACGGGT

mRNA sequence

AAACGTGAGCCATTCACACAGCCAAGGATCATTTTTTCAAAAACCCTTCATTTGTTATTATACAGCTTGAAAGAGCGTCTCATCCAACTCAAAATTTGTTCTATTACTTCATTTCCCGCCTCTTTTGTTCGTCTAATCTCGATCCCCAGGCATGGAACCCAACAGGGTTTTGCTGGATTTTCAAAATCCGATCCAAGATGCCATCTCCACAATCCAATTCGCTCCCCGTTCGAACAATCTCCTTATTTCTTCCTGGGATTCTAGTCTTCGTTTGTACGATGTGGATAATTCTGCACTCAGACTTGAAGCTCCCTCCGAAGCTGCACTGCTTGACTGCTGCTTCCAGGACGAGTCACTGGCTTTGAGCGCTGGTTCTGATGGCTTAATAAGAAGGTATGACTTGCAATCAGGAATTTCTGAAACCATTGGAAAACATGATGATATTGCAACCAATATCAGATATTCGGATCAAACATGCCAAATAGTAACAGCGGGTTTGGATGGAAAGATACTGTTGTGGGACACGCGTAGAGAAAAATGTGTGTCATTTGTGAGAAACAGGGGTTCAGATGTGGTGTCCATGTCTATCTCTGGATTTAATTTAATTGTGGCTTCTGGAATATGTGTATATTCACTGGACTTACGTAACATACAAAAACCAGTTCAACTCACAGATTCTTGTATGAAAGTTCCAGTAGCTTGTGTAAGCTCTGTTCCTTATACTGAAGGATTTGCGATTGGCTCAGTAGATGGACGTGTAGCTTTGGAGATTGCTTGTCTGGACCAAGCAGATGACATCAGATATATTTTCTGGTGTCACCCAAAGTCAAAGGGTAGAAGAAAGCATTTGGAGTCGGTGAATGATATTGTGTTTAATCCAATATTTCCAAATAGCGTGGCATCGTTGTCGTACAACTGCGGAGGTCAACTTTTGGCTGTTGCTTCAAGTTGTACGTATCAAGAAGCTAAAGAAAGAGAAGATGCTCCTCAAGTGTTCATACATGTAATGGAGGAGTTCAATCCCAAATCTTTCTCTGCCTCTTCCACTGGATAGCTTCACCTTTCCTACCATGCCTTGCTTCCCACTAAAGGTAATAAATTATTAGATTTTATGTCAAATTTATAGATGGAATATCCGAAACGGGT

Coding sequence (CDS)

ATGGAACCCAACAGGGTTTTGCTGGATTTTCAAAATCCGATCCAAGATGCCATCTCCACAATCCAATTCGCTCCCCGTTCGAACAATCTCCTTATTTCTTCCTGGGATTCTAGTCTTCGTTTGTACGATGTGGATAATTCTGCACTCAGACTTGAAGCTCCCTCCGAAGCTGCACTGCTTGACTGCTGCTTCCAGGACGAGTCACTGGCTTTGAGCGCTGGTTCTGATGGCTTAATAAGAAGGTATGACTTGCAATCAGGAATTTCTGAAACCATTGGAAAACATGATGATATTGCAACCAATATCAGATATTCGGATCAAACATGCCAAATAGTAACAGCGGGTTTGGATGGAAAGATACTGTTGTGGGACACGCGTAGAGAAAAATGTGTGTCATTTGTGAGAAACAGGGGTTCAGATGTGGTGTCCATGTCTATCTCTGGATTTAATTTAATTGTGGCTTCTGGAATATGTGTATATTCACTGGACTTACGTAACATACAAAAACCAGTTCAACTCACAGATTCTTGTATGAAAGTTCCAGTAGCTTGTGTAAGCTCTGTTCCTTATACTGAAGGATTTGCGATTGGCTCAGTAGATGGACGTGTAGCTTTGGAGATTGCTTGTCTGGACCAAGCAGATGACATCAGATATATTTTCTGGTGTCACCCAAAGTCAAAGGGTAGAAGAAAGCATTTGGAGTCGGTGAATGATATTGTGTTTAATCCAATATTTCCAAATAGCGTGGCATCGTTGTCGTACAACTGCGGAGGTCAACTTTTGGCTGTTGCTTCAAGTTGTACGTATCAAGAAGCTAAAGAAAGAGAAGATGCTCCTCAAGTGTTCATACATGTAATGGAGGAGTTCAATCCCAAATCTTTCTCTGCCTCTTCCACTGGATAG

Protein sequence

MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKVPVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIVFNPIFPNSVASLSYNCGGQLLAVASSCTYQEAKEREDAPQVFIHVMEEFNPKSFSASSTG

Homology

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy Swiss-Prot
Match: F4I241 (Mitotic checkpoint protein BUB3.3 OS=Arabidopsis thaliana OX=3702 GN=BUB3.3 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.3e-75
Identity = 140/307 (45.60%), Postives = 207/307 (67.43%), Query Frame = 0


Query: 8   LDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDE 67
           L+F+NPI+DA+S ++F+P+SNNLL++SWDS LRLYDV++S+L LE  S+AALLDCCF++E
Sbjct: 6   LEFENPIEDAVSRLRFSPQSNNLLVASWDSYLRLYDVESSSLSLELNSQAALLDCCFENE 65

Query: 68  SLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTRR 127
           S + ++GSDG IRRYDL +G  +TIG+HDDI+T+I YS +  ++++ G D KI  WDTR+
Sbjct: 66  STSFTSGSDGFIRRYDLNAGTVDTIGRHDDISTSIVYSYEKGEVISTGFDEKIKFWDTRQ 125

Query: 128 EKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKVPVACVSS 187
            + + F  + G  V  +++SG NL+V     ++  DLRN+ +  Q   S ++VP+ C++S
Sbjct: 126 RESLVFSTDAGGAVGCVTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITS 185

Query: 188 VPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIVFNPI--- 247
           VPY+ G+A+GSVDGRVA++      + +I+Y F CHPKS+  R     +N I F+P    
Sbjct: 186 VPYSRGYAVGSVDGRVAVDFPNTSCSSEIKYSFRCHPKSRNGRLDGVCINAIEFSPCGSG 245

Query: 248 ---------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAKERED 285
                                      + NS+ASL+++  G+LLA+ASS TYQ+AKE+E+
Sbjct: 246 TFVTGDNEGYVISWNAKSRRRLNELPRYSNSIASLAFDHTGELLAIASSHTYQDAKEKEE 305

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy Swiss-Prot
Match: Q9LJN8 (Mitotic checkpoint protein BUB3.1 OS=Arabidopsis thaliana OX=3702 GN=BUB3.1 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 7.4e-44
Identity = 108/319 (33.86%), Postives = 162/319 (50.78%), Query Frame = 0


Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD  +RLYDV  ++L+ E     A+LDCCF D+ 
Sbjct: 11  ELSNPPSDGISNLRFSNNSDHLLVSSWDKRVRLYDVSTNSLKGEFLHGGAVLDCCFHDDF 70

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTR-- 128
              S G+D  +RR     G  + +G HD     + YS    Q++T   D  +  WD R  
Sbjct: 71  SGFSVGADYKVRRIVFNVGKEDILGTHDKAVRCVEYSYAAGQVITGSWDKTVKCWDPRGA 130

Query: 129 --REKCVSFVRNRGSDVVSMSISGFNLIVA-SGICVYSLDLRNIQKPVQLTDSCMKVPVA 188
              E+       +   V SMS+ G  L+VA +G  V   DLRN+ +P Q  +S +K    
Sbjct: 131 SGPERTQVGTYLQPERVYSMSLVGHRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 190

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIVFN 248
           CV   P   G+A+ SV+GRVA+E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 191 CVRCYPNGTGYALSSVEGRVAMEFFDLSEAAQAKKYAFKCHRKSEAGRDIVYPVNSIAFH 250

Query: 249 PI-----------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAK 293
           PI                             +P S+++LS++  GQLLAVASS T++E +
Sbjct: 251 PIYGTFATGGCDGFVNIWDGNNKKRLYQYSKYPTSISALSFSRDGQLLAVASSYTFEEGE 310

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy Swiss-Prot
Match: Q9C701 (Mitotic checkpoint protein BUB3.2 OS=Arabidopsis thaliana OX=3702 GN=BUB3.2 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.8e-42
Identity = 107/319 (33.54%), Postives = 160/319 (50.16%), Query Frame = 0


Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD S+RLYD +   +R E     A+LDCCF D+S
Sbjct: 10  ELSNPPSDGISNLRFSNNSDHLLVSSWDKSVRLYDANGDLMRGEFKHGGAVLDCCFHDDS 69

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTR-- 128
              S  +D  +RR D  +G  + +G H+     + YS    Q++T   D  I  WD R  
Sbjct: 70  SGFSVCADTKVRRIDFNAGKEDVLGTHEKPVRCVEYSYAAGQVITGSWDKTIKCWDPRGA 129

Query: 129 --REKCVSFVRNRGSDVVSMSISGFNLIVA-SGICVYSLDLRNIQKPVQLTDSCMKVPVA 188
              E+       +   V S+S+ G  L+VA +G  V   DLRN+ +P Q  +S +K    
Sbjct: 130 SGTERTQIGTYMQPERVNSLSLVGNRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 189

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIVFN 248
           CV   P   G+A+ SV+GRV++E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 190 CVRCYPNGTGYALSSVEGRVSMEFFDLSEAAQAKKYAFKCHRKSEDGRDIVYPVNAIAFH 249

Query: 249 PI-----------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAK 293
           PI                             +P S+A+LS++  G LLAVASS T++E  
Sbjct: 250 PIYGTFASGGCDGFVNIWDGNNKKRLYQYSKYPTSIAALSFSRDGGLLAVASSYTFEEGD 309

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy Swiss-Prot
Match: Q54DM1 (Mitotic checkpoint protein bub3 OS=Dictyostelium discoideum OX=44689 GN=bub3 PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.1e-38
Identity = 90/316 (28.48%), Postives = 156/316 (49.37%), Query Frame = 0


Query: 2   EPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLD 61
           EP     + + P  D IS++ F P S NLL++SWDS++R YD  N+  + +   +  ++D
Sbjct: 4   EPTSSFYELRLPPSDGISSVNFCPNSVNLLVTSWDSTVRCYDTQNNVQKWQYNHKGPVMD 63

Query: 62  CCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKIL 121
            CF +++   S    G ++ YD  +G+ + +G H+D   ++ Y+  T Q+ T G D ++ 
Sbjct: 64  GCFPEKNKVFSGDVFGSVKHYDPVAGVEKEVGSHEDGVRSVVYNSDTQQLFTGGWDQQLK 123

Query: 122 LWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGIC---VYSLDLRNIQKPVQLTDSCM 181
           LWD R +K      +  S + +M +S  + ++  G     V   D R ++  +Q  +S +
Sbjct: 124 LWDIRSDKMEISNHDLQSKIFTMDVSPISNMLVIGTADKYVTIYDTRQMETHLQKRESSI 183

Query: 182 KVPVACVSSVPYTEGFAIGSVDGRVALEIACLDQA-DDIRYIFWCHPKSKGRRKHLESVN 241
           K    C+ +    +G+A+ SV+GR+A+E      A    +Y F CH  ++     +  VN
Sbjct: 184 KYQTRCIRTFTDGKGYALASVEGRIAMEYFDPSPAVQSKKYAFKCHRLTESGVDVVYPVN 243

Query: 242 DIVFNP-----------------------------IFPNSVASLSYNCGGQLLAVASSCT 285
            I FNP                              +P S++S+S+N  G +LAVASS T
Sbjct: 244 CIAFNPHYGTFATGGCDKNVFFWDGANRKRLHALKTYPTSISSMSFNTDGNILAVASSYT 303

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy Swiss-Prot
Match: Q1JQB2 (Mitotic checkpoint protein BUB3 OS=Bos taurus OX=9913 GN=BUB3 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.1e-34
Identity = 98/317 (30.91%), Postives = 158/317 (49.84%), Query Frame = 0


Query: 13  PIQDAISTIQFAPRSNN-LLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDESLAL 72
           P +D IS+++F+P ++  LL+SSWD+S+RLYDV  +++RL+     A+LDC F D + A 
Sbjct: 12  PPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAW 71

Query: 73  SAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTRREKCV 132
           S G D  ++ +DL +     +G HD     + Y  +   +VT   D  + LWD  R  C 
Sbjct: 72  SGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDP-RTPCN 131

Query: 133 SFVRNRGSDVVSMSISGFNLIV-ASGICVYSLDLRNIQKPVQLTDSCMKVPVACVSSVPY 192
           +   ++   V ++S+SG  LIV  +G  V   DLRN+    Q  +S +K    C+ + P 
Sbjct: 132 AGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPN 191

Query: 193 TEGFAIGSVDGRVALEIACLDQADDI---RYIFWCHPKSKGRRKHLESVNDIVFNPI--- 252
            +G+ + S++GRVA+E   LD + ++   +Y F CH   +   + +  VN I F+ I   
Sbjct: 192 KQGYVLSSIEGRVAVEY--LDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNT 251

Query: 253 --------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAKEREDA 294
                                     +P S+ASL+++  G  LA+ASS  Y+        
Sbjct: 252 FATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPE 311

[Close]

BLAST of Cp4.1LG07g06420 vs. NCBI nr
Match: XP_023537047.1 (mitotic checkpoint protein BUB3.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 586 bits (1510), Expect = 2.88e-210
Identity = 300/330 (90.91%), Postives = 300/330 (90.91%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV
Sbjct: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 300
           FNPI                              FPNSVASLSYNCGGQLLAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQLLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. NCBI nr
Match: XP_022951111.1 (mitotic checkpoint protein BUB3.3 [Cucurbita moschata])

HSP 1 Score: 571 bits (1472), Expect = 1.78e-204
Identity = 290/330 (87.88%), Postives = 296/330 (89.70%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNI YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASG+CVYSLD RN+QKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGVCVYSLDFRNMQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDI 
Sbjct: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 300
           FNPI                              FPNSVASLSYNCGGQ+LAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. NCBI nr
Match: KAG7020766.1 (Mitotic checkpoint protein BUB3.3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 569 bits (1466), Expect = 1.46e-203
Identity = 289/330 (87.58%), Postives = 296/330 (89.70%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNI YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNR SDVVSMSISGFNLI+ASG+CVYSLDLRN+QKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLILASGVCVYSLDLRNMQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDI 
Sbjct: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 300
           FNPI                              FPNSVASLSYNCGGQ+LAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. NCBI nr
Match: KAG6585860.1 (Mitotic checkpoint protein BUB3.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 568 bits (1464), Expect = 2.94e-203
Identity = 289/330 (87.58%), Postives = 296/330 (89.70%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLL+FQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLNFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNI YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNR SDVVSMSISGFNLIVASG+CVYSLDLRN+QKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRDSDVVSMSISGFNLIVASGVCVYSLDLRNMQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDI 
Sbjct: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 300
           FNPI                              FPNSVASLSYNCGGQ+LAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. NCBI nr
Match: XP_022973311.1 (mitotic checkpoint protein BUB3.3 [Cucurbita maxima])

HSP 1 Score: 567 bits (1462), Expect = 5.94e-203
Identity = 289/330 (87.58%), Postives = 295/330 (89.39%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAP SNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPCSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNI YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASG+CVYSLDLRNIQKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGVCVYSLDLRNIQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPYTEGFA+GSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDI 
Sbjct: 181 PVACVSSVPYTEGFAVGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 300
           FNPI                              FPNSVASLSYNCGGQ+LAVASSCTYQ
Sbjct: 241 FNPIHPGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy TrEMBL
Match: A0A6J1GGN0 (mitotic checkpoint protein BUB3.3 OS=Cucurbita moschata OX=3662 GN=LOC111454056 PE=4 SV=1)

HSP 1 Score: 571 bits (1472), Expect = 8.61e-205
Identity = 290/330 (87.88%), Postives = 296/330 (89.70%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNI YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASG+CVYSLD RN+QKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGVCVYSLDFRNMQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDI 
Sbjct: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 300
           FNPI                              FPNSVASLSYNCGGQ+LAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy TrEMBL
Match: A0A6J1I8A9 (mitotic checkpoint protein BUB3.3 OS=Cucurbita maxima OX=3661 GN=LOC111471870 PE=4 SV=1)

HSP 1 Score: 567 bits (1462), Expect = 2.88e-203
Identity = 289/330 (87.58%), Postives = 295/330 (89.39%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           MEPNRVLLDFQNPIQDAISTIQFAP SNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL
Sbjct: 1   MEPNRVLLDFQNPIQDAISTIQFAPCSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNI YSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNISYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASG+CVYSLDLRNIQKPVQLTDSCMKV
Sbjct: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGVCVYSLDLRNIQKPVQLTDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPYTEGFA+GSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDI 
Sbjct: 181 PVACVSSVPYTEGFAVGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIG 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 300
           FNPI                              FPNSVASLSYNCGGQ+LAVASSCTYQ
Sbjct: 241 FNPIHPGAFATGDNAGFVSVWDFQSKRRILELPRFPNSVASLSYNCGGQVLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy TrEMBL
Match: A0A6J1E115 (mitotic checkpoint protein BUB3.3-like OS=Momordica charantia OX=3673 GN=LOC111025428 PE=4 SV=1)

HSP 1 Score: 474 bits (1219), Expect = 2.06e-166
Identity = 241/320 (75.31%), Postives = 263/320 (82.19%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           ME NRVLLDFQ    DAISTIQFAP SNNL++SSWDSSLRLYDVD+SALRLEAPSEAALL
Sbjct: 1   MEMNRVLLDFQ----DAISTIQFAPHSNNLIVSSWDSSLRLYDVDSSALRLEAPSEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQDES+A SAGSDG IRRYDLQSGI +TIGKHDDIAT+I YS +TCQIV+AGLDGKI
Sbjct: 61  DCCFQDESVAFSAGSDGFIRRYDLQSGILDTIGKHDDIATHIGYSGRTCQIVSAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
           LLWDTR  K +S VRN GSDVVSMS+SGFNLIVA+G+CVYS DLR+++KP+QL DSCMKV
Sbjct: 121 LLWDTRTVKALSIVRNMGSDVVSMSLSGFNLIVATGVCVYSFDLRSLEKPIQLKDSCMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPY EG A+GSVDGRVALEI+CLD+ADDIRYIFWCHPKSK RR HLESVN I 
Sbjct: 181 PVACVSSVPYCEGHAVGSVDGRVALEISCLDRADDIRYIFWCHPKSKARRNHLESVNAIA 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 290
           FNPI                              FPNSVASLSYNCGG+LLAVASSCT+Q
Sbjct: 241 FNPIHHGAFATGDNEGFVSMWDFHSKRRLLELPRFPNSVASLSYNCGGELLAVASSCTFQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy TrEMBL
Match: A0A0A0KNE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G468480 PE=4 SV=1)

HSP 1 Score: 453 bits (1165), Expect = 3.29e-158
Identity = 232/318 (72.96%), Postives = 252/318 (79.25%), Query Frame = 0


Query: 1   MEPNRVLLDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALL 60
           ME NRVLLDFQ PIQDA+S +QFAP SNNLL+SSWDS LRLYD DN  LRLE  SEAALL
Sbjct: 1   MESNRVLLDFQIPIQDAVSRLQFAPLSNNLLVSSWDSILRLYDADNCTLRLEVASEAALL 60

Query: 61  DCCFQDESLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKI 120
           DCCFQ+ESLALSA SDG IRRY+LQSGI ET+GKH D  T+IRYSDQTCQIVTAGLDGKI
Sbjct: 61  DCCFQNESLALSAASDGCIRRYELQSGIFETVGKHGDSVTHIRYSDQTCQIVTAGLDGKI 120

Query: 121 LLWDTRREKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKV 180
            LWDTR +K  SFVRN GSDVVSMS+SGFNLIVASG CVY LDLRN++K +QL DS MKV
Sbjct: 121 QLWDTRNKKSPSFVRNMGSDVVSMSLSGFNLIVASGACVYLLDLRNLEKSIQLKDSYMKV 180

Query: 181 PVACVSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIV 240
           PVACVSSVPY EG A+GSVDGRVAL+IACLDQ DDIRYIF CHPKSK  + HLESVNDIV
Sbjct: 181 PVACVSSVPYREGVAVGSVDGRVALDIACLDQTDDIRYIFRCHPKSKAAKNHLESVNDIV 240

Query: 241 FNPI------------------------------FPNSVASLSYNCGGQLLAVASSCTYQ 288
           FNPI                              FPNSVASLSYNCGG+LLAVASSCTYQ
Sbjct: 241 FNPIHHGAFATGDNAGFVSIWDFQRKTRILELPRFPNSVASLSYNCGGELLAVASSCTYQ 300

[Close]

BLAST of Cp4.1LG07g06420 vs. ExPASy TrEMBL
Match: A0A540LL68 (Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_027262 PE=4 SV=1)

HSP 1 Score: 378 bits (970), Expect = 2.17e-128
Identity = 194/322 (60.25%), Postives = 236/322 (73.29%), Query Frame = 0


Query: 8   LDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDE 67
           L F+NPI+DAIS I+FAP+S+NLLISSWDSSLRLYDV +S LRLEAPSEAALLDCCFQDE
Sbjct: 6   LKFENPIRDAISRIRFAPKSDNLLISSWDSSLRLYDVGSSQLRLEAPSEAALLDCCFQDE 65

Query: 68  SLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTRR 127
           S+A +AGSDG+IRRYDL SGI ++IG HDD++T + YS++T Q++TAGLD KIL WDTR 
Sbjct: 66  SVAFAAGSDGVIRRYDLHSGIHDSIGNHDDMSTCVGYSEETSQLITAGLDRKILSWDTRT 125

Query: 128 EKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKVPVACVSS 187
           EK   F  N  S+V+SMS+SG +++VA G  +Y  DLRN+ KP+Q  DS M V + CVSS
Sbjct: 126 EKGFVFASNLDSEVMSMSLSGLDMMVAIGSSIYVYDLRNLDKPIQSKDSHMDVKIVCVSS 185

Query: 188 VPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIVFNPI--- 247
           +PY +GFA+GS+DGRVALEI+C   +DDIRY+F CHPKS  +R HLESVNDIVFNP+   
Sbjct: 186 IPYAKGFAVGSIDGRVALEISCPSSSDDIRYMFRCHPKSTDKRYHLESVNDIVFNPLMYG 245

Query: 248 ---------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAKERED 299
                                      +PNSVASLSYN  GQLLAVASS TYQEA E E+
Sbjct: 246 AFVTGDNDGYVTAWDAKSKKRLFQLPRYPNSVASLSYNHRGQLLAVASSYTYQEANEIEE 305

[Close]

BLAST of Cp4.1LG07g06420 vs. TAIR 10
Match: AT1G69400.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 284.6 bits (727), Expect = 8.9e-77
Identity = 140/307 (45.60%), Postives = 207/307 (67.43%), Query Frame = 0


Query: 8   LDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDE 67
           L+F+NPI+DA+S ++F+P+SNNLL++SWDS LRLYDV++S+L LE  S+AALLDCCF++E
Sbjct: 6   LEFENPIEDAVSRLRFSPQSNNLLVASWDSYLRLYDVESSSLSLELNSQAALLDCCFENE 65

Query: 68  SLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTRR 127
           S + ++GSDG IRRYDL +G  +TIG+HDDI+T+I YS +  ++++ G D KI  WDTR+
Sbjct: 66  STSFTSGSDGFIRRYDLNAGTVDTIGRHDDISTSIVYSYEKGEVISTGFDEKIKFWDTRQ 125

Query: 128 EKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKVPVACVSS 187
            + + F  + G  V  +++SG NL+V     ++  DLRN+ +  Q   S ++VP+ C++S
Sbjct: 126 RESLVFSTDAGGAVGCVTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITS 185

Query: 188 VPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIVFNPI--- 247
           VPY+ G+A+GSVDGRVA++      + +I+Y F CHPKS+  R     +N I F+P    
Sbjct: 186 VPYSRGYAVGSVDGRVAVDFPNTSCSSEIKYSFRCHPKSRNGRLDGVCINAIEFSPCGSG 245

Query: 248 ---------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAKERED 285
                                      + NS+ASL+++  G+LLA+ASS TYQ+AKE+E+
Sbjct: 246 TFVTGDNEGYVISWNAKSRRRLNELPRYSNSIASLAFDHTGELLAIASSHTYQDAKEKEE 305

[Close]

BLAST of Cp4.1LG07g06420 vs. TAIR 10
Match: AT1G69400.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 115/245 (46.94%), Postives = 175/245 (71.43%), Query Frame = 0


Query: 8   LDFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDE 67
           L+F+NPI+DA+S ++F+P+SNNLL++SWDS LRLYDV++S+L LE  S+AALLDCCF++E
Sbjct: 6   LEFENPIEDAVSRLRFSPQSNNLLVASWDSYLRLYDVESSSLSLELNSQAALLDCCFENE 65

Query: 68  SLALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTRR 127
           S + ++GSDG IRRYDL +G  +TIG+HDDI+T+I YS +  ++++ G D KI  WDTR+
Sbjct: 66  STSFTSGSDGFIRRYDLNAGTVDTIGRHDDISTSIVYSYEKGEVISTGFDEKIKFWDTRQ 125

Query: 128 EKCVSFVRNRGSDVVSMSISGFNLIVASGICVYSLDLRNIQKPVQLTDSCMKVPVACVSS 187
            + + F  + G  V  +++SG NL+V     ++  DLRN+ +  Q   S ++VP+ C++S
Sbjct: 126 RESLVFSTDAGGAVGCVTVSGNNLVVCVDASMHIYDLRNLDEAFQSYASQVEVPIRCITS 185

Query: 188 VPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIVFNPIFPN 247
           VPY+ G+A+GSVDGRVA++      + +I+Y F CHPKS+  R     +N I F+P + +
Sbjct: 186 VPYSRGYAVGSVDGRVAVDFPNTSCSSEIKYSFRCHPKSRNGRLDGVCINAIEFSPWYMS 245

Query: 248 SVASL 253
            +  L
Sbjct: 246 LIHHL 250

[Close]

BLAST of Cp4.1LG07g06420 vs. TAIR 10
Match: AT3G19590.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 179.1 bits (453), Expect = 5.3e-45
Identity = 108/319 (33.86%), Postives = 162/319 (50.78%), Query Frame = 0


Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD  +RLYDV  ++L+ E     A+LDCCF D+ 
Sbjct: 11  ELSNPPSDGISNLRFSNNSDHLLVSSWDKRVRLYDVSTNSLKGEFLHGGAVLDCCFHDDF 70

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTR-- 128
              S G+D  +RR     G  + +G HD     + YS    Q++T   D  +  WD R  
Sbjct: 71  SGFSVGADYKVRRIVFNVGKEDILGTHDKAVRCVEYSYAAGQVITGSWDKTVKCWDPRGA 130

Query: 129 --REKCVSFVRNRGSDVVSMSISGFNLIVA-SGICVYSLDLRNIQKPVQLTDSCMKVPVA 188
              E+       +   V SMS+ G  L+VA +G  V   DLRN+ +P Q  +S +K    
Sbjct: 131 SGPERTQVGTYLQPERVYSMSLVGHRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 190

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIVFN 248
           CV   P   G+A+ SV+GRVA+E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 191 CVRCYPNGTGYALSSVEGRVAMEFFDLSEAAQAKKYAFKCHRKSEAGRDIVYPVNSIAFH 250

Query: 249 PI-----------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAK 293
           PI                             +P S+++LS++  GQLLAVASS T++E +
Sbjct: 251 PIYGTFATGGCDGFVNIWDGNNKKRLYQYSKYPTSISALSFSRDGQLLAVASSYTFEEGE 310

[Close]

BLAST of Cp4.1LG07g06420 vs. TAIR 10
Match: AT1G49910.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.3e-43
Identity = 107/319 (33.54%), Postives = 160/319 (50.16%), Query Frame = 0


Query: 9   DFQNPIQDAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAALLDCCFQDES 68
           +  NP  D IS ++F+  S++LL+SSWD S+RLYD +   +R E     A+LDCCF D+S
Sbjct: 10  ELSNPPSDGISNLRFSNNSDHLLVSSWDKSVRLYDANGDLMRGEFKHGGAVLDCCFHDDS 69

Query: 69  LALSAGSDGLIRRYDLQSGISETIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTR-- 128
              S  +D  +RR D  +G  + +G H+     + YS    Q++T   D  I  WD R  
Sbjct: 70  SGFSVCADTKVRRIDFNAGKEDVLGTHEKPVRCVEYSYAAGQVITGSWDKTIKCWDPRGA 129

Query: 129 --REKCVSFVRNRGSDVVSMSISGFNLIVA-SGICVYSLDLRNIQKPVQLTDSCMKVPVA 188
              E+       +   V S+S+ G  L+VA +G  V   DLRN+ +P Q  +S +K    
Sbjct: 130 SGTERTQIGTYMQPERVNSLSLVGNRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 189

Query: 189 CVSSVPYTEGFAIGSVDGRVALEIACLDQADDI-RYIFWCHPKSKGRRKHLESVNDIVFN 248
           CV   P   G+A+ SV+GRV++E   L +A    +Y F CH KS+  R  +  VN I F+
Sbjct: 190 CVRCYPNGTGYALSSVEGRVSMEFFDLSEAAQAKKYAFKCHRKSEDGRDIVYPVNAIAFH 249

Query: 249 PI-----------------------------FPNSVASLSYNCGGQLLAVASSCTYQEAK 293
           PI                             +P S+A+LS++  G LLAVASS T++E  
Sbjct: 250 PIYGTFASGGCDGFVNIWDGNNKKRLYQYSKYPTSIAALSFSRDGGLLAVASSYTFEEGD 309

[Close]

BLAST of Cp4.1LG07g06420 vs. TAIR 10
Match: AT1G80670.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 1.3e-14
Identity = 58/240 (24.17%), Postives = 110/240 (45.83%), Query Frame = 0


Query: 16  DAISTIQFAPRSNNLLISSWDSSLRLYDVDNSALRLEAPSEAA-------LLDCCFQDES 75
           D+IS++ F+PR++ L+ +SWD+ +R +++  S   L +  +A+       L      D +
Sbjct: 26  DSISSLSFSPRADILVATSWDNQVRCWEISRSGASLASAPKASISHDQPVLCSAWKDDGT 85

Query: 76  LALSAGSDGLIRRYDLQSGISE-TIGKHDDIATNIRYSDQTCQIVTAGLDGKILLWDTRR 135
              S G D   + + L SG    T+  H+     + +      + T   D  +  WDTR+
Sbjct: 86  TVFSGGCDKQAKMWPLLSGGQPVTVAMHEGPIAAMAWIPGMNLLATGSWDKTLKYWDTRQ 145

Query: 136 EKCVSFVRNRGSDVVSMSISGFNLIVAS---GICVYSLDLRNIQKPVQLTDSCMKVPVAC 195
           +  V   +       ++S+    ++V +    + V+  +L+N Q   +   S +K    C
Sbjct: 146 QNPV-HTQQLPDKCYTLSVKHPLMVVGTADRNLIVF--NLQNPQTEFKRIQSPLKYQTRC 205

Query: 196 VSSVPYTEGFAIGSVDGRVALEIACLDQADDIRYIFWCHPKSKGRRKHLESVNDIVFNPI 245
           V++ P  +GF +GS++GRV +     D      + F CH         + SVN + F+P+
Sbjct: 206 VTAFPDQQGFLVGSIEGRVGVH-HLDDSQQSKNFTFKCHRDG----NDIYSVNSLNFHPV 257

The following BLAST results are available for this feature:

BLAST of Cp4.1LG07g06420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I241	1.3e-75	45.60	Mitotic checkpoint protein BUB3.3 OS=Arabidopsis thaliana OX=3702 GN=BUB3.3 PE=2...	[more]
Q9LJN8	7.4e-44	33.86	Mitotic checkpoint protein BUB3.1 OS=Arabidopsis thaliana OX=3702 GN=BUB3.1 PE=1...	[more]
Q9C701	1.8e-42	33.54	Mitotic checkpoint protein BUB3.2 OS=Arabidopsis thaliana OX=3702 GN=BUB3.2 PE=2...	[more]
Q54DM1	6.1e-38	28.48	Mitotic checkpoint protein bub3 OS=Dictyostelium discoideum OX=44689 GN=bub3 PE=...	[more]
Q1JQB2	3.1e-34	30.91	Mitotic checkpoint protein BUB3 OS=Bos taurus OX=9913 GN=BUB3 PE=2 SV=1	[more]

BLAST of Cp4.1LG07g06420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537047.1	2.88e-210	90.91	mitotic checkpoint protein BUB3.3 [Cucurbita pepo subsp. pepo]	[more]
XP_022951111.1	1.78e-204	87.88	mitotic checkpoint protein BUB3.3 [Cucurbita moschata]	[more]
KAG7020766.1	1.46e-203	87.58	Mitotic checkpoint protein BUB3.3 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6585860.1	2.94e-203	87.58	Mitotic checkpoint protein BUB3.3, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022973311.1	5.94e-203	87.58	mitotic checkpoint protein BUB3.3 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG07g06420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GGN0	8.61e-205	87.88	mitotic checkpoint protein BUB3.3 OS=Cucurbita moschata OX=3662 GN=LOC111454056 ...	[more]
A0A6J1I8A9	2.88e-203	87.58	mitotic checkpoint protein BUB3.3 OS=Cucurbita maxima OX=3661 GN=LOC111471870 PE...	[more]
A0A6J1E115	2.06e-166	75.31	mitotic checkpoint protein BUB3.3-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0KNE2	3.29e-158	72.96	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G468480 PE=4 SV=1	[more]
A0A540LL68	2.17e-128	60.25	Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_027262 PE=4 SV=1	[more]

BLAST of Cp4.1LG07g06420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69400.1	8.9e-77	45.60	Transducin/WD40 repeat-like superfamily protein	[more]
AT1G69400.2	1.2e-65	46.94	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G19590.1	5.3e-45	33.86	Transducin/WD40 repeat-like superfamily protein	[more]
AT1G49910.1	1.3e-43	33.54	Transducin/WD40 repeat-like superfamily protein	[more]
AT1G80670.1	1.3e-14	24.17	Transducin/WD40 repeat-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 3..43 e-value: 0.57 score: 18.5 coord: 85..124 e-value: 0.032 score: 23.4 coord: 44..83 e-value: 21.0 score: 8.7
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 92..133 score: 10.775796
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 4..293 e-value: 3.1E-47 score: 163.7
None	No IPR available	PANTHER	PTHR10971	MRNA EXPORT FACTOR AND BUB3	coord: 5..244
None	No IPR available	PANTHER	PTHR10971:SF17	MITOTIC CHECKPOINT PROTEIN BUB3.3	coord: 5..244
None	No IPR available	PANTHER	PTHR10971	MRNA EXPORT FACTOR AND BUB3	coord: 243..291
None	No IPR available	PANTHER	PTHR10971:SF17	MITOTIC CHECKPOINT PROTEIN BUB3.3	coord: 243..291
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 111..125
IPR036322	WD40-repeat-containing domain superfamily	SUPERFAMILY	50978	WD40 repeat-like	coord: 12..264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG07g06420.1	Cp4.1LG07g06420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding