Cp4.1LG07g01930 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g01930
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiongibberellin 3-beta-dioxygenase 1-like
LocationCp4.1LG07: 1733401 .. 1734131 (+)
RNA-Seq ExpressionCp4.1LG07g01930
SyntenyCp4.1LG07g01930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACGTACACAATTAGAGTTTACCACTCTATGCATATAAATTAGGGCTTACCACTTACCCGTTCAAATATTTTCGTAGTTTAAACTAAATTTGAGATTAAAATATATTTCTATACACCATCATTAAAATATTCGTACTAGAAGTGAAACTCTCGGTATTGTTTATCACAAAAATCTAATTAATTACATAAACTCATATTATCTTTTATTGAATATACGTCGAACTATTTTTATCCTGCTCATTACTTTTTTTTTTTTTTTTTTTTTTTACTCGGGTAAGTCCTACACCGCCCTCCAACTGAACTCGTATCCACCGTGCCCTGATCCAGACCAAGTCATGGGCCTTGCTGCCCACACCGACACCTCCCTCTTCACCATCGTTCACCAGGCATGCAATGACGGCCTCCAAATCTTCAAAGACAAAGCAGGCTGGATTCCGTTGTCTCCGATGAGAGGTTCTTTCGTTGTCAACGTTGGCGACCTTCTCCACATTATGTCGAACGGGCGGTTTCGGAGCATTCTTCATCGGGTTACGATACAAGGGAAGAAGGTGCATAGATTTTCATTGGCATATTTCTATTATCCACCACCTTATTTATATATTACACCTCATTGCAAGGCATTGAGTGAATCACCACAAACTCCTCTCTATCGGTGTGTGAGTGTGAAAGAATATTTCACCATGAAGGCCAAAAACAATGGGGAGGGACTTTCTACCATCAAAATATGA

mRNA sequence

ATGCACTCCTACACCGCCCTCCAACTGAACTCGTATCCACCGTGCCCTGATCCAGACCAAGTCATGGGCCTTGCTGCCCACACCGACACCTCCCTCTTCACCATCGTTCACCAGGCATGCAATGACGGCCTCCAAATCTTCAAAGACAAAGCAGGCTGGATTCCGTTGTCTCCGATGAGAGGTTCTTTCGTTGTCAACGTTGGCGACCTTCTCCACATTATGTCGAACGGGCGGTTTCGGAGCATTCTTCATCGGGTTACGATACAAGGGAAGAAGGTGCATAGATTTTCATTGGCATATTTCTATTATCCACCACCTTATTTATATATTACACCTCATTGCAAGGCATTGAGTGAATCACCACAAACTCCTCTCTATCGGTGTGTGAGTGTGAAAGAATATTTCACCATGAAGGCCAAAAACAATGGGGAGGGACTTTCTACCATCAAAATATGA

Coding sequence (CDS)

ATGCACTCCTACACCGCCCTCCAACTGAACTCGTATCCACCGTGCCCTGATCCAGACCAAGTCATGGGCCTTGCTGCCCACACCGACACCTCCCTCTTCACCATCGTTCACCAGGCATGCAATGACGGCCTCCAAATCTTCAAAGACAAAGCAGGCTGGATTCCGTTGTCTCCGATGAGAGGTTCTTTCGTTGTCAACGTTGGCGACCTTCTCCACATTATGTCGAACGGGCGGTTTCGGAGCATTCTTCATCGGGTTACGATACAAGGGAAGAAGGTGCATAGATTTTCATTGGCATATTTCTATTATCCACCACCTTATTTATATATTACACCTCATTGCAAGGCATTGAGTGAATCACCACAAACTCCTCTCTATCGGTGTGTGAGTGTGAAAGAATATTTCACCATGAAGGCCAAAAACAATGGGGAGGGACTTTCTACCATCAAAATATGA

Protein sequence

MHSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTIKI
Homology
BLAST of Cp4.1LG07g01930 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 4.1e-35
Identity = 72/146 (49.32%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 6   ALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVV 65
           ALQLNSYP CPDPD  MGLA HTD++  TI+ Q    GLQ+ ++ +GWI + P++G  VV
Sbjct: 210 ALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQGGLVV 269

Query: 66  NVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQTPL 125
           NVGDL HI+SNG + S+LHRV +   +  RFS+AY Y PP  + I PH K +  + + PL
Sbjct: 270 NVGDLFHILSNGLYPSVLHRVLVNRTR-QRFSVAYLYGPPSNVEICPHAKLIGPT-KPPL 329

Query: 126 YRCVSVKEYFTMKAKNNGEGLSTIKI 152
           YR V+  EY   KAK+  + LS++++
Sbjct: 330 YRSVTWNEYLGTKAKHFNKALSSVRL 353

BLAST of Cp4.1LG07g01930 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 6.6e-33
Identity = 70/144 (48.61%), Postives = 98/144 (68.06%), Query Frame = 0

Query: 7   LQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVVN 66
           +QLN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ+F+D  GW+   P+ GS VVN
Sbjct: 205 IQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVN 264

Query: 67  VGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQTPLY 126
           VGDLLHI++NG F S+LHR  +   +  RFS+AY + PP  + I+P  K L +  Q+PLY
Sbjct: 265 VGDLLHILTNGIFPSVLHRARVNHVR-SRFSMAYLWGPPSDIMISPLPK-LVDPLQSPLY 324

Query: 127 RCVSVKEYFTMKAKNNGEGLSTIK 151
             ++ K+Y   KA +  + LS I+
Sbjct: 325 PSLTWKQYLATKATHFNQSLSIIR 346

BLAST of Cp4.1LG07g01930 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-32
Identity = 70/145 (48.28%), Postives = 98/145 (67.59%), Query Frame = 0

Query: 6   ALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVV 65
           ALQLN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ+F+D  GW+ + P  GS VV
Sbjct: 211 ALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVV 270

Query: 66  NVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQTPL 125
           NVGDL HI+SNG F+S+LHR  +   +  R S+A+ + P   + I+P  K +S   ++PL
Sbjct: 271 NVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQSDIKISPVPKLVS-PVESPL 330

Query: 126 YRCVSVKEYFTMKAKNNGEGLSTIK 151
           Y+ V+ KEY   KA +  + LS I+
Sbjct: 331 YQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of Cp4.1LG07g01930 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 59/147 (40.14%), Postives = 96/147 (65.31%), Query Frame = 0

Query: 6   ALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVV 65
           A++LN YP CP+P++ MGLAAHTD+++ TI+HQ+   GLQ+F++++GW+ + P  G  VV
Sbjct: 206 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVV 265

Query: 66  NVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFY-YPPPYLYITPHCKALSESPQTP 125
           N+GDL HI+SNG+  S++HR  +   +  R S+AY +  P   + I P  K L+   +  
Sbjct: 266 NIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISK-LTGPAEPS 325

Query: 126 LYRCVSVKEYFTMKAKNNGEGLSTIKI 152
           LYR ++ KEY  +K +   + +  I++
Sbjct: 326 LYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of Cp4.1LG07g01930 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.1e-26
Identity = 67/147 (45.58%), Postives = 89/147 (60.54%), Query Frame = 0

Query: 7   LQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQI---FKDKAGWIPLSPMRGSF 66
           LQLNSYP CPDP   MGLA HTD+SL TI++Q    GL+I    ++ + WI + P+ GS 
Sbjct: 206 LQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSL 265

Query: 67  VVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQT 126
           VV +GDL HI+SNG+FRS +HR  +  K  HR S AYF  PP  L I P     S+    
Sbjct: 266 VVIMGDLSHIISNGQFRSTMHRAVV-NKTHHRVSAAYFAGPPKNLQIGP---LTSDKNHP 325

Query: 127 PLYRCVSVKEYFTMKAKNNGEGLSTIK 151
           P+YR +  +EY   KA +  + L+  +
Sbjct: 326 PIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of Cp4.1LG07g01930 vs. NCBI nr
Match: XP_023522671.1 (gibberellin 3-beta-dioxygenase 1-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 312 bits (800), Expect = 3.54e-107
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 0

Query: 3   SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 62
           SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS
Sbjct: 22  SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 81

Query: 63  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 122
           FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ
Sbjct: 82  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 141

Query: 123 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           TPLYRCVSVKEYFTMKAKNNGEGLSTIKI
Sbjct: 142 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 170

BLAST of Cp4.1LG07g01930 vs. NCBI nr
Match: XP_023537854.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 312 bits (800), Expect = 1.19e-104
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 0

Query: 3   SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 62
           SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS
Sbjct: 193 SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 252

Query: 63  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 122
           FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ
Sbjct: 253 FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 312

Query: 123 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           TPLYRCVSVKEYFTMKAKNNGEGLSTIKI
Sbjct: 313 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 341

BLAST of Cp4.1LG07g01930 vs. NCBI nr
Match: XP_022951873.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 308 bits (789), Expect = 5.58e-103
Identity = 145/149 (97.32%), Postives = 148/149 (99.33%), Query Frame = 0

Query: 3   SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 62
           SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGW+PLSPMRGS
Sbjct: 193 SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWVPLSPMRGS 252

Query: 63  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 122
           FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYI+PHCK LSESPQ
Sbjct: 253 FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYISPHCKPLSESPQ 312

Query: 123 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           TPLYRCVSVKEYFTMKAKNNGEGLST+KI
Sbjct: 313 TPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of Cp4.1LG07g01930 vs. NCBI nr
Match: KAG6585440.1 (Gibberellin 3-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 303 bits (776), Expect = 5.26e-101
Identity = 144/149 (96.64%), Postives = 147/149 (98.66%), Query Frame = 0

Query: 3   SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 62
           SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGW+PLSPMRGS
Sbjct: 193 SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWVPLSPMRGS 252

Query: 63  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 122
           FVVNVGDLLHIMSNGRF+SILHRVTIQGKKVHRFSLAYFYYPPPYLYI+PH K LSESPQ
Sbjct: 253 FVVNVGDLLHIMSNGRFQSILHRVTIQGKKVHRFSLAYFYYPPPYLYISPHSKPLSESPQ 312

Query: 123 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           TPLYRCVSVKEYFTMKAKNNGEGLSTIKI
Sbjct: 313 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 341

BLAST of Cp4.1LG07g01930 vs. NCBI nr
Match: XP_023002882.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 297 bits (761), Expect = 9.96e-99
Identity = 140/149 (93.96%), Postives = 144/149 (96.64%), Query Frame = 0

Query: 3   SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 62
           SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQ CNDGLQIFKD+A W+PLSPMRGS
Sbjct: 193 SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCNDGLQIFKDRASWVPLSPMRGS 252

Query: 63  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 122
           FVVNVGDLLHIMSNGRF SILHRVTIQGKKVHRFSLAYFYYPPPYLYI+PHCK LSESPQ
Sbjct: 253 FVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFYYPPPYLYISPHCKPLSESPQ 312

Query: 123 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
            PLYRCVSV+EYFTMKAKNNGEGLSTIKI
Sbjct: 313 APLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of Cp4.1LG07g01930 vs. ExPASy TrEMBL
Match: A0A6J1GIY0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454608 PE=3 SV=1)

HSP 1 Score: 308 bits (789), Expect = 2.70e-103
Identity = 145/149 (97.32%), Postives = 148/149 (99.33%), Query Frame = 0

Query: 3   SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 62
           SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGW+PLSPMRGS
Sbjct: 193 SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWVPLSPMRGS 252

Query: 63  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 122
           FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYI+PHCK LSESPQ
Sbjct: 253 FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYISPHCKPLSESPQ 312

Query: 123 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           TPLYRCVSVKEYFTMKAKNNGEGLST+KI
Sbjct: 313 TPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of Cp4.1LG07g01930 vs. ExPASy TrEMBL
Match: A0A6J1KKU0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496619 PE=3 SV=1)

HSP 1 Score: 297 bits (761), Expect = 4.82e-99
Identity = 140/149 (93.96%), Postives = 144/149 (96.64%), Query Frame = 0

Query: 3   SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGS 62
           SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQ CNDGLQIFKD+A W+PLSPMRGS
Sbjct: 193 SYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCNDGLQIFKDRASWVPLSPMRGS 252

Query: 63  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 122
           FVVNVGDLLHIMSNGRF SILHRVTIQGKKVHRFSLAYFYYPPPYLYI+PHCK LSESPQ
Sbjct: 253 FVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFYYPPPYLYISPHCKPLSESPQ 312

Query: 123 TPLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
            PLYRCVSV+EYFTMKAKNNGEGLSTIKI
Sbjct: 313 APLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of Cp4.1LG07g01930 vs. ExPASy TrEMBL
Match: A0A6J1BUZ4 (gibberellin 3-beta-dioxygenase 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004962 PE=3 SV=1)

HSP 1 Score: 253 bits (645), Expect = 1.99e-81
Identity = 120/148 (81.08%), Postives = 132/148 (89.19%), Query Frame = 0

Query: 4   YTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSF 63
           YTAL+LNSYPPCPDP QVMGLAAHTDTSLFTI+HQA  DGLQ+FKD+ GWIPLSPM G+ 
Sbjct: 197 YTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFKDEVGWIPLSPMSGTL 256

Query: 64  VVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQT 123
           VVN+GDLLHI+SNGRFRSILHRV IQGK+ HR S+AYFYYPP  LYI+PHC  LSESPQT
Sbjct: 257 VVNIGDLLHILSNGRFRSILHRVVIQGKE-HRLSMAYFYYPPNELYISPHCNPLSESPQT 316

Query: 124 PLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           PLYRCV V+EYF+MKAKN G GLSTIKI
Sbjct: 317 PLYRCVKVREYFSMKAKNMGRGLSTIKI 343

BLAST of Cp4.1LG07g01930 vs. ExPASy TrEMBL
Match: A0A6J1BSK2 (gibberellin 3-beta-dioxygenase 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004962 PE=3 SV=1)

HSP 1 Score: 253 bits (645), Expect = 2.39e-81
Identity = 120/148 (81.08%), Postives = 132/148 (89.19%), Query Frame = 0

Query: 4   YTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSF 63
           YTAL+LNSYPPCPDP QVMGLAAHTDTSLFTI+HQA  DGLQ+FKD+ GWIPLSPM G+ 
Sbjct: 203 YTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFKDEVGWIPLSPMSGTL 262

Query: 64  VVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQT 123
           VVN+GDLLHI+SNGRFRSILHRV IQGK+ HR S+AYFYYPP  LYI+PHC  LSESPQT
Sbjct: 263 VVNIGDLLHILSNGRFRSILHRVVIQGKE-HRLSMAYFYYPPNELYISPHCNPLSESPQT 322

Query: 124 PLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           PLYRCV V+EYF+MKAKN G GLSTIKI
Sbjct: 323 PLYRCVKVREYFSMKAKNMGRGLSTIKI 349

BLAST of Cp4.1LG07g01930 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 239 bits (610), Expect = 4.71e-76
Identity = 113/148 (76.35%), Postives = 127/148 (85.81%), Query Frame = 0

Query: 4   YTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSF 63
           Y AL+LNSYPPCPDP +VMGLAAHTDTSLFTIVHQA +DGLQ+FKD  GW+PLSPM G+ 
Sbjct: 202 YMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHDGLQVFKDGTGWVPLSPMSGTL 261

Query: 64  VVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQT 123
           +VNVGD+LHI+SNGRF SILHRV I   K HRFSLAYFY PP  LYI+P+CK LSESPQ 
Sbjct: 262 IVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFYDPPGDLYISPYCKPLSESPQI 321

Query: 124 PLYRCVSVKEYFTMKAKNNGEGLSTIKI 151
           PLYRCV+VKEYFT+KAK  G+GLS IKI
Sbjct: 322 PLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of Cp4.1LG07g01930 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 141.7 bits (356), Expect = 4.7e-34
Identity = 70/144 (48.61%), Postives = 98/144 (68.06%), Query Frame = 0

Query: 7   LQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVVN 66
           +QLN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ+F+D  GW+   P+ GS VVN
Sbjct: 205 IQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVN 264

Query: 67  VGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQTPLY 126
           VGDLLHI++NG F S+LHR  +   +  RFS+AY + PP  + I+P  K L +  Q+PLY
Sbjct: 265 VGDLLHILTNGIFPSVLHRARVNHVR-SRFSMAYLWGPPSDIMISPLPK-LVDPLQSPLY 324

Query: 127 RCVSVKEYFTMKAKNNGEGLSTIK 151
             ++ K+Y   KA +  + LS I+
Sbjct: 325 PSLTWKQYLATKATHFNQSLSIIR 346

BLAST of Cp4.1LG07g01930 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 140.6 bits (353), Expect = 1.0e-33
Identity = 70/145 (48.28%), Postives = 98/145 (67.59%), Query Frame = 0

Query: 6   ALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVV 65
           ALQLN YP CP+PD+ MGLAAHTD++L TI++Q    GLQ+F+D  GW+ + P  GS VV
Sbjct: 211 ALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVV 270

Query: 66  NVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQTPL 125
           NVGDL HI+SNG F+S+LHR  +   +  R S+A+ + P   + I+P  K +S   ++PL
Sbjct: 271 NVGDLFHILSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQSDIKISPVPKLVS-PVESPL 330

Query: 126 YRCVSVKEYFTMKAKNNGEGLSTIK 151
           Y+ V+ KEY   KA +  + LS I+
Sbjct: 331 YQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of Cp4.1LG07g01930 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 124.4 bits (311), Expect = 7.8e-29
Identity = 59/147 (40.14%), Postives = 96/147 (65.31%), Query Frame = 0

Query: 6   ALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQIFKDKAGWIPLSPMRGSFVV 65
           A++LN YP CP+P++ MGLAAHTD+++ TI+HQ+   GLQ+F++++GW+ + P  G  VV
Sbjct: 206 AIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVV 265

Query: 66  NVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFY-YPPPYLYITPHCKALSESPQTP 125
           N+GDL HI+SNG+  S++HR  +   +  R S+AY +  P   + I P  K L+   +  
Sbjct: 266 NIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISK-LTGPAEPS 325

Query: 126 LYRCVSVKEYFTMKAKNNGEGLSTIKI 152
           LYR ++ KEY  +K +   + +  I++
Sbjct: 326 LYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of Cp4.1LG07g01930 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 120.2 bits (300), Expect = 1.5e-27
Identity = 67/147 (45.58%), Postives = 89/147 (60.54%), Query Frame = 0

Query: 7   LQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGLQI---FKDKAGWIPLSPMRGSF 66
           LQLNSYP CPDP   MGLA HTD+SL TI++Q    GL+I    ++ + WI + P+ GS 
Sbjct: 206 LQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSL 265

Query: 67  VVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQT 126
           VV +GDL HI+SNG+FRS +HR  +  K  HR S AYF  PP  L I P     S+    
Sbjct: 266 VVIMGDLSHIISNGQFRSTMHRAVV-NKTHHRVSAAYFAGPPKNLQIGP---LTSDKNHP 325

Query: 127 PLYRCVSVKEYFTMKAKNNGEGLSTIK 151
           P+YR +  +EY   KA +  + L+  +
Sbjct: 326 PIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of Cp4.1LG07g01930 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 109.4 bits (272), Expect = 2.6e-24
Identity = 57/133 (42.86%), Postives = 86/133 (64.66%), Query Frame = 0

Query: 4   YTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACN-DGLQIFKDKAGWIPLSPMRGS 63
           + ++++N YPPCP PDQVMGL  H+D +  TI+ Q    +GLQI KD   W+ + P+R +
Sbjct: 208 WQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQVEGLQIKKD-GKWVVVKPLRDA 267

Query: 64  FVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYPPPYLYITPHCKALSESPQ 123
            VVNVG++L I++NGR+RSI HR  +  +K  R S+A F+ P     I P  K+L +  +
Sbjct: 268 LVVNVGEILEIITNGRYRSIEHRAVVNSEK-ERLSVAMFHSPGKETIIRP-AKSLVDRQK 327

Query: 124 TPLYRCVSVKEYF 136
             L++ +S +EYF
Sbjct: 328 QCLFKSMSTQEYF 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O246484.1e-3549.32Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1[more]
Q9ZT846.6e-3348.61Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ... [more]
Q391031.5e-3248.28Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ... [more]
Q9C9711.1e-2740.14Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ... [more]
Q9SVS82.1e-2645.58Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_023522671.13.54e-107100.00gibberellin 3-beta-dioxygenase 1-like, partial [Cucurbita pepo subsp. pepo][more]
XP_023537854.11.19e-104100.00gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022951873.15.58e-10397.32gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata][more]
KAG6585440.15.26e-10196.64Gibberellin 3-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023002882.19.96e-9993.96gibberellin 3-beta-dioxygenase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GIY02.70e-10397.32gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454... [more]
A0A6J1KKU04.82e-9993.96gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11149661... [more]
A0A6J1BUZ41.99e-8181.08gibberellin 3-beta-dioxygenase 1-like isoform X2 OS=Momordica charantia OX=3673 ... [more]
A0A6J1BSK22.39e-8181.08gibberellin 3-beta-dioxygenase 1-like isoform X1 OS=Momordica charantia OX=3673 ... [more]
Q9LM064.71e-7676.35Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE... [more]
Match NameE-valueIdentityDescription
AT1G80340.14.7e-3448.61gibberellin 3-oxidase 2 [more]
AT1G15550.11.0e-3348.28gibberellin 3-oxidase 1 [more]
AT1G80330.17.8e-2940.14gibberellin 3-oxidase 4 [more]
AT4G21690.11.5e-2745.58gibberellin 3-oxidase 3 [more]
AT1G78550.12.6e-2442.862-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 7..102
e-value: 5.0E-26
score: 91.1
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..149
e-value: 1.2E-41
score: 145.0
NoneNo IPR availablePANTHERPTHR47991:SF77GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKEcoord: 4..142
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 4..142
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 3..147
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 3..104
score: 12.072969

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g01930.1Cp4.1LG07g01930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding