Cp4.1LG07g01250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG07g01250
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	ethylene-responsive transcription factor RAP2-4-like
Location	Cp4.1LG07: 705295 .. 706413 (+)
RNA-Seq Expression	Cp4.1LG07g01250
Synteny	Cp4.1LG07g01250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGCTTTGATGGATTTCTATGGTTGCAGAGATCTTCAGTCGTATCCAGTTGGTGGTGAATTAATGGAAGCGCTTGAACCTTTTATGAAAGCTGCTTCCTCTTCTTCTTCTTCTTTCCTTCCTTCCTTTTCTCCTTCTTCTTCATCTTTCGTTCCTGATTTCTCCTCTTCTTTCTCTTCAATCCAAACGGAGTCCACTTCTTATCCTTCCGATTGCTCGCCGCCGGTGGCCTATTCATATTCCGACGGGTTTTCGGCTAACAACCTTCACGGTTTGGAGCAATCGGCTTCAATCGGACTCAACTACTTGACTCCCTTTCAGATCCAACAGATCCAATCTCAATTCGGTCTTCAGACTCAGATCCAGCCGGTTTGGGGACATGTTAACAACCAGCAAGAGCTAACGAAAAACCAGATGAACGGCGTGAATTTGCTTGGCCCGAAGGCGATTCCGATGAAACATGCGGGATCTCCACCGAAATCGACGAAGCTTTATCGAGGCGTGAGGCAAAGGCATTGGGGAAAATGGGTGGCTGAGATCCGTCTACCGAGGAACCGTACTCGGCTATGGCTAGGCACCTTCGACACCGCTGAAGAAGCCGCTTTAGCCTATGACAAAGCCGCCTACAAGCTCCGAGGCGATTTCGCCAGGCTTAATTTCCCTCAGCTGAAGCACCACGGCTCCTCCGTCGGCGGCGATTTCGGCGAGTACAAGCCGCTCCACTCCGCGGTCGATGCTAAGCTCGATGCCATATGCCAAACCTTGGCTGAGTCGCAAAAACAGGGGAAATCGAACCGATCTGCCTCCTCGAGGAAGTCGAAATCATCATCGATTTCTTCCACGGTCGGATCGGAGTCTCAGGCACCGACGGTGATCGATTCTGACGACGATCAGAAGGCTAGCGATGTCAGGTCGAGTGGATCGGAGATCTGTTGCAAGGCGGAGAACTCCTCGTCGCCGGTTTCAGCCGAGAGCGACGAGTCAGCAGGATCTTCGCCTTTATCAGATCTGACGTTCCCCGATCCGACAGATTCGCCATGGGAACAGCCGTCAGAAAGCTCTATGTTGCAGAAATATCCGTCTGAAATCGATTGGGCTTCGATTTTCACTTCATGA

mRNA sequence

ATGCCTGCTTTGATGGATTTCTATGGTTGCAGAGATCTTCAGTCGTATCCAGTTGGTGGTGAATTAATGGAAGCGCTTGAACCTTTTATGAAAGCTGCTTCCTCTTCTTCTTCTTCTTTCCTTCCTTCCTTTTCTCCTTCTTCTTCATCTTTCGTTCCTGATTTCTCCTCTTCTTTCTCTTCAATCCAAACGGAGTCCACTTCTTATCCTTCCGATTGCTCGCCGCCGGTGGCCTATTCATATTCCGACGGGTTTTCGGCTAACAACCTTCACGGTTTGGAGCAATCGGCTTCAATCGGACTCAACTACTTGACTCCCTTTCAGATCCAACAGATCCAATCTCAATTCGGTCTTCAGACTCAGATCCAGCCGGTTTGGGGACATGTTAACAACCAGCAAGAGCTAACGAAAAACCAGATGAACGGCGTGAATTTGCTTGGCCCGAAGGCGATTCCGATGAAACATGCGGGATCTCCACCGAAATCGACGAAGCTTTATCGAGGCGTGAGGCAAAGGCATTGGGGAAAATGGGTGGCTGAGATCCGTCTACCGAGGAACCGTACTCGGCTATGGCTAGGCACCTTCGACACCGCTGAAGAAGCCGCTTTAGCCTATGACAAAGCCGCCTACAAGCTCCGAGGCGATTTCGCCAGGCTTAATTTCCCTCAGCTGAAGCACCACGGCTCCTCCGTCGGCGGCGATTTCGGCGAGTACAAGCCGCTCCACTCCGCGGTCGATGCTAAGCTCGATGCCATATGCCAAACCTTGGCTGAGTCGCAAAAACAGGGGAAATCGAACCGATCTGCCTCCTCGAGGAAGTCGAAATCATCATCGATTTCTTCCACGGTCGGATCGGAGTCTCAGGCACCGACGGTGATCGATTCTGACGACGATCAGAAGGCTAGCGATGTCAGGTCGAGTGGATCGGAGATCTGTTGCAAGGCGGAGAACTCCTCGTCGCCGGTTTCAGCCGAGAGCGACGAGTCAGCAGGATCTTCGCCTTTATCAGATCTGACGTTCCCCGATCCGACAGATTCGCCATGGGAACAGCCGTCAGAAAGCTCTATGTTGCAGAAATATCCGTCTGAAATCGATTGGGCTTCGATTTTCACTTCATGA

Coding sequence (CDS)

ATGCCTGCTTTGATGGATTTCTATGGTTGCAGAGATCTTCAGTCGTATCCAGTTGGTGGTGAATTAATGGAAGCGCTTGAACCTTTTATGAAAGCTGCTTCCTCTTCTTCTTCTTCTTTCCTTCCTTCCTTTTCTCCTTCTTCTTCATCTTTCGTTCCTGATTTCTCCTCTTCTTTCTCTTCAATCCAAACGGAGTCCACTTCTTATCCTTCCGATTGCTCGCCGCCGGTGGCCTATTCATATTCCGACGGGTTTTCGGCTAACAACCTTCACGGTTTGGAGCAATCGGCTTCAATCGGACTCAACTACTTGACTCCCTTTCAGATCCAACAGATCCAATCTCAATTCGGTCTTCAGACTCAGATCCAGCCGGTTTGGGGACATGTTAACAACCAGCAAGAGCTAACGAAAAACCAGATGAACGGCGTGAATTTGCTTGGCCCGAAGGCGATTCCGATGAAACATGCGGGATCTCCACCGAAATCGACGAAGCTTTATCGAGGCGTGAGGCAAAGGCATTGGGGAAAATGGGTGGCTGAGATCCGTCTACCGAGGAACCGTACTCGGCTATGGCTAGGCACCTTCGACACCGCTGAAGAAGCCGCTTTAGCCTATGACAAAGCCGCCTACAAGCTCCGAGGCGATTTCGCCAGGCTTAATTTCCCTCAGCTGAAGCACCACGGCTCCTCCGTCGGCGGCGATTTCGGCGAGTACAAGCCGCTCCACTCCGCGGTCGATGCTAAGCTCGATGCCATATGCCAAACCTTGGCTGAGTCGCAAAAACAGGGGAAATCGAACCGATCTGCCTCCTCGAGGAAGTCGAAATCATCATCGATTTCTTCCACGGTCGGATCGGAGTCTCAGGCACCGACGGTGATCGATTCTGACGACGATCAGAAGGCTAGCGATGTCAGGTCGAGTGGATCGGAGATCTGTTGCAAGGCGGAGAACTCCTCGTCGCCGGTTTCAGCCGAGAGCGACGAGTCAGCAGGATCTTCGCCTTTATCAGATCTGACGTTCCCCGATCCGACAGATTCGCCATGGGAACAGCCGTCAGAAAGCTCTATGTTGCAGAAATATCCGTCTGAAATCGATTGGGCTTCGATTTTCACTTCATGA

Protein sequence

MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKYPSEIDWASIFTS

Homology

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy Swiss-Prot
Match: Q8H1E4 (Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana OX=3702 GN=RAP2-4 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 7.5e-70
Identity = 189/385 (49.09%), Postives = 231/385 (60.00%), Query Frame = 0


Query: 1   MPALMDFYGC-RDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSF 60
           M A M+ Y C R  Q    GGELM+AL PF+K+ S S SS   S + S+S+F+     S 
Sbjct: 1   MAAAMNLYTCSRSFQD--SGGELMDALVPFIKSVSDSPSS---SSAASASAFL---HPSA 60

Query: 61  SSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSAS-IGLNYLTPFQIQQIQSQFGL 120
            S+      YP        +SY           L+Q+ S IGLN L+  QI QIQSQ  +
Sbjct: 61  FSLPPLPGYYPDSTFLTQPFSYGS--------DLQQTGSLIGLNNLSSSQIHQIQSQ--I 120

Query: 121 QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKH-----------AGSPPKSTKLYR 180
              + P   H NN    +       NLL PK + MK            +G P K TKLYR
Sbjct: 121 HHPLPPT--HHNNNNSFS-------NLLSPKPLLMKQSGVAGSCFAYGSGVPSKPTKLYR 180

Query: 181 GVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHH 240
           GVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFP L+H+
Sbjct: 181 GVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPNLRHN 240

Query: 241 GSSVGGDFGEYKPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSS--ISSTVGS 300
           GS +GGDFGEYKPLHS+VDAKL+AIC+++AE+QKQ KS +S+  R+ K SS  +S  V +
Sbjct: 241 GSHIGGDFGEYKPLHSSVDAKLEAICKSMAETQKQDKSTKSSKKREKKVSSPDLSEKVKA 300

Query: 301 ESQAPTVIDSDDDQKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTD 360
           E  + ++                          S PV+   + +AGSSPLSDLTF DP +
Sbjct: 301 EENSVSI------------------------GGSPPVTEFEESTAGSSPLSDLTFADPEE 332

Query: 361 SPWEQPSESSMLQKYPS-EIDWASI 370
            P  Q +E+  L+KYPS EIDW SI
Sbjct: 361 PP--QWNETFSLEKYPSYEIDWDSI 332

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy Swiss-Prot
Match: Q9LM15 (Ethylene-responsive transcription factor RAP2-13 OS=Arabidopsis thaliana OX=3702 GN=RAP2-13 PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.0e-50
Identity = 159/374 (42.51%), Postives = 192/374 (51.34%), Query Frame = 0


Query: 1   MPALMDFYGCRDL-QSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSF 60
           M   MDFY  +   QS P GGELMEAL PF+K+ S+ SS+F                   
Sbjct: 1   MTTSMDFYSNKTFQQSDPFGGELMEALLPFIKSPSNDSSAF------------------- 60

Query: 61  SSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQ 120
                 + S P+    P++Y        ++LH                            
Sbjct: 61  ------AFSLPA----PISY-------GSDLHSFSHH----------------------- 120

Query: 121 TQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAG-SPPKSTKLYRGVRQRHWGKWV 180
                                     L PK + MK  G S  K TKLYRGVRQRHWGKWV
Sbjct: 121 --------------------------LSPKPVSMKQTGTSAAKPTKLYRGVRQRHWGKWV 180

Query: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY 240
           AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFP L+H+         EY
Sbjct: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPDLRHN--------DEY 240

Query: 241 KPLHSAVDAKLDAICQTLAE-SQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDD 300
           +PL S+VDAKL+AICQ LAE +QKQ +S + +SSRK +SS+++  +  E           
Sbjct: 241 QPLQSSVDAKLEAICQNLAETTQKQVRSTKKSSSRK-RSSTVAVKLPEE----------- 253

Query: 301 DQKASDVRSSGSEICCKAENSSSPVSAESDESAG-SSPLSDLTFPDPTDSPWEQPSESSM 360
                D  S+G          SSP+  ES  S G SSPLS+LTF D T+   + P   + 
Sbjct: 301 -----DYSSAG----------SSPLLTESYGSGGSSSPLSELTFGD-TEEEIQPPWNENA 253

Query: 361 LQKYPS-EIDWASI 370
           L+KYPS EIDW SI
Sbjct: 361 LEKYPSYEIDWDSI 253

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy Swiss-Prot
Match: Q9SKW5 (Ethylene-responsive transcription factor ERF055 OS=Arabidopsis thaliana OX=3702 GN=ERF055 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.7e-45
Identity = 155/363 (42.70%), Postives = 201/363 (55.37%), Query Frame = 0


Query: 19  GGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVP---DFSSSFSSIQTESTSYPSDCSP 78
           GGELMEAL+PF K+AS+S+S+  P+F+ S+ +F     D  SS S     ++ +PS  + 
Sbjct: 10  GGELMEALQPFYKSASTSASN--PAFASSNDAFASAPNDLFSSSSYYNPHASLFPSHSTT 69

Query: 79  PVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQTQIQPVWGHVNNQQEL 138
               SY D +S +    +   +S G +   P   +  QSQF  Q  I   + H +N   +
Sbjct: 70  ----SYPDIYSGS----MTYPSSFGSDLQQP---ENYQSQFHYQNTI--TYTHQDNNTCM 129

Query: 139 TKNQMNGVNLLGPK-----AIPMKHAGSPPKSTKLYRGVRQRHWGKWVAEIRLPRNRTRL 198
                  +N + P        P   +GS  K  KLYRGVRQRHWGKWVAEIRLPRNRTRL
Sbjct: 130 -------LNFIEPSQPGFMTQPGPSSGSVSKPAKLYRGVRQRHWGKWVAEIRLPRNRTRL 189

Query: 199 WLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKPLHSAVDAKLD 258
           WLGTFDTAEEAALAYD+AA+KLRGD ARLNFP L++   S   D GEY P+ +AVDAKL+
Sbjct: 190 WLGTFDTAEEAALAYDRAAFKLRGDSARLNFPALRYQTGSSPSDTGEYGPIQAAVDAKLE 249

Query: 259 AICQTLAESQKQ-GKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQKASDVRSSGS 318
           AI   LAE + Q GK+ R  +SRK   ++ SS                +Q ++  + SG 
Sbjct: 250 AI---LAEPKNQPGKTER--TSRKRAKAAASSA---------------EQPSAPQQHSG- 309

Query: 319 EICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKYPS-EIDWAS 372
                        S ESD S   SP SD+   +    P    +E+ ML K PS EIDWAS
Sbjct: 310 -------------SGESDGS--GSPTSDVMVQEMCQEPEMPWNENFMLGKCPSYEIDWAS 314

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy Swiss-Prot
Match: O65665 (Ethylene-responsive transcription factor ERF060 OS=Arabidopsis thaliana OX=3702 GN=ERF060 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.3e-41
Identity = 125/285 (43.86%), Postives = 161/285 (56.49%), Query Frame = 0


Query: 87  ANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQTQIQPVWGHVNNQQELTKNQMNGVNLL 146
           +N + G  Q+ S+GLN LTP+QI QIQ+Q   +  I            ++ N       L
Sbjct: 37  SNTVFGFNQTTSLGLNQLTPYQIHQIQNQLNQRRNI------------ISPN-------L 96

Query: 147 GPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYD 206
            PK +PMK+      + KLYRGVRQRHWGKWVAEIRLP+NRTRLWLGTFDTAEEAA+AYD
Sbjct: 97  APKPVPMKNM----TAQKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAMAYD 156

Query: 207 KAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKPLHSAVDAKLDAICQTLAESQKQGKSN 266
            AAYKLRG+FARLNFPQ +H     GG    + PLHS+VDAKL  ICQ+L          
Sbjct: 157 LAAYKLRGEFARLNFPQFRHEDGYYGGG-SCFNPLHSSVDAKLQEICQSL---------- 216

Query: 267 RSASSRKSKSSSISSTVGSESQAPTVIDSDDDQKASDVRSSGSEICCKAENSSSPVSAES 326
                RK++   +     SE++         + +   +RS         ENS S    + 
Sbjct: 217 -----RKTEDIDLPC---SETELFPPKTEYQESEYGFLRSD--------ENSFS----DE 266

Query: 327 DESAGSSPLSDL-TFPDPTDSPWEQPSESSMLQKYPS-EIDWASI 370
                SSP S + TF D +DS +++   S  L+K+PS EIDW +I
Sbjct: 277 SHVESSSPESGITTFLDFSDSGFDEIG-SFGLEKFPSVEIDWDAI 266

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy Swiss-Prot
Match: Q9SIE4 (Ethylene-responsive transcription factor ERF056 OS=Arabidopsis thaliana OX=3702 GN=ERF056 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.4e-36
Identity = 119/287 (41.46%), Postives = 160/287 (55.75%), Query Frame = 0


Query: 88  NNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQTQIQPVWGHVNNQQELTKNQMNGVNLLG 147
           N   G+ +S  +GLN LTP+QI QIQ+Q             +N+++    N       L 
Sbjct: 12  NTSFGVNKSMPLGLNQLTPYQIHQIQNQ-------------LNHRRSTISN-------LS 71

Query: 148 PKAIPMKH-AGSPPKSTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYD 207
           P  I MK+   S  K+  LYRGVRQRHWGKWVAEIRLP+NRTRLWLGTF+TAE+AALAYD
Sbjct: 72  PNRIRMKNLTPSTSKTKNLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFETAEKAALAYD 131

Query: 208 KAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKPLHSAVDAKLDAICQTLAESQKQGKSN 267
           +AA++LRGD A+LNFP L H          +  PL S+VD KL AIC++L ++++     
Sbjct: 132 QAAFQLRGDIAKLNFPNLIHE---------DMNPLPSSVDTKLQAICKSLRKTEE----I 191

Query: 268 RSASSRKSKSSSISSTVGSESQAPTVIDSDDDQKASDVRSSGSEICCKAENSSSPVSAES 327
            S S +  + S  S +  +E   P         KA         +     ++ SP S E+
Sbjct: 192 CSVSDQTKEYSVYSVSDKTELFLP---------KAELFLPKREHLETNELSNESPRSDET 251

Query: 328 ---DESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKYPS-EIDWASI 370
              DES      SD TF D +D+ +E+   S  L+K+PS EIDW +I
Sbjct: 252 SLLDESQAEYSSSDKTFLDFSDTEFEEIG-SFGLRKFPSVEIDWDAI 255

[Close]

BLAST of Cp4.1LG07g01250 vs. NCBI nr
Match: XP_023537287.1 (ethylene-responsive transcription factor RAP2-4-like [Cucurbita pepo subsp. pepo] >AXF54219.1 AP2-3 [Cucurbita pepo])

HSP 1 Score: 721 bits (1861), Expect = 8.05e-262
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60
           MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS
Sbjct: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60

Query: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120
           SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT
Sbjct: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120

Query: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180
           QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE
Sbjct: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180

Query: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240
           IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP
Sbjct: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240

Query: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300
           LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK
Sbjct: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300

Query: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360
           ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY
Sbjct: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360

Query: 361 PSEIDWASIFTS 372
           PSEIDWASIFTS
Sbjct: 361 PSEIDWASIFTS 372

[Close]

BLAST of Cp4.1LG07g01250 vs. NCBI nr
Match: KAG7020206.1 (Ethylene-responsive transcription factor RAP2-4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 716 bits (1849), Expect = 5.43e-260
Identity = 370/372 (99.46%), Postives = 370/372 (99.46%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60
           M ALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS
Sbjct: 1   MAALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60

Query: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120
           SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT
Sbjct: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120

Query: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180
           QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE
Sbjct: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180

Query: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240
           IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP
Sbjct: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240

Query: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300
           LHSAVDAKLDAICQTLAESQKQGKSNRS SSRKSKSSSISSTVGSESQAPTVIDSDDDQK
Sbjct: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSVSSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300

Query: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360
           ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY
Sbjct: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360

Query: 361 PSEIDWASIFTS 372
           PSEIDWASIFTS
Sbjct: 361 PSEIDWASIFTS 372

[Close]

BLAST of Cp4.1LG07g01250 vs. NCBI nr
Match: XP_022951481.1 (ethylene-responsive transcription factor RAP2-4-like [Cucurbita moschata])

HSP 1 Score: 713 bits (1840), Expect = 1.38e-258
Identity = 371/374 (99.20%), Postives = 371/374 (99.20%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSS--FLPSFSPSSSSFVPDFSSS 60
           M ALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSS  FLPSFSPSSSSFVPDFSSS
Sbjct: 1   MAALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSSSFLPSFSPSSSSFVPDFSSS 60

Query: 61  FSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGL 120
           FSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGL
Sbjct: 61  FSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGL 120

Query: 121 QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWV 180
           QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWV
Sbjct: 121 QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWV 180

Query: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY 240
           AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY
Sbjct: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY 240

Query: 241 KPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD 300
           KPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD
Sbjct: 241 KPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD 300

Query: 301 QKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQ 360
           QKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQ
Sbjct: 301 QKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQ 360

Query: 361 KYPSEIDWASIFTS 372
           KYPSEIDWASIFTS
Sbjct: 361 KYPSEIDWASIFTS 374

[Close]

BLAST of Cp4.1LG07g01250 vs. NCBI nr
Match: XP_023001968.1 (ethylene-responsive transcription factor RAP2-4-like [Cucurbita maxima])

HSP 1 Score: 702 bits (1812), Expect = 2.28e-254
Identity = 365/372 (98.12%), Postives = 366/372 (98.39%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60
           M ALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSS FLPSFSPSSSSF PDFSSSFS
Sbjct: 1   MAALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSS-FLPSFSPSSSSFDPDFSSSFS 60

Query: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120
           SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT
Sbjct: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120

Query: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180
           QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKH GSPPKSTKLYRGVRQRHWGKWVAE
Sbjct: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHVGSPPKSTKLYRGVRQRHWGKWVAE 180

Query: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240
           IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP
Sbjct: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240

Query: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300
           LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD K
Sbjct: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDLK 300

Query: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360
           A +VRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY
Sbjct: 301 AGEVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360

Query: 361 PSEIDWASIFTS 372
           PSEIDWASIFTS
Sbjct: 361 PSEIDWASIFTS 371

[Close]

BLAST of Cp4.1LG07g01250 vs. NCBI nr
Match: XP_038885142.1 (ethylene-responsive transcription factor RAP2-4-like [Benincasa hispida])

HSP 1 Score: 560 bits (1443), Expect = 2.03e-198
Identity = 304/373 (81.50%), Postives = 319/373 (85.52%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60
           M ALMDFYGCR++QS P GGELMEALEPFMK ASSS   + PS SP       DFSSS S
Sbjct: 1   MAALMDFYGCREIQSDPFGGELMEALEPFMKVASSS---YFPSSSP-------DFSSS-S 60

Query: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120
           SIQTESTSYPSD SPP+ + +S GFS  + HG+EQSASIGLNYLTPFQIQQIQSQFGLQT
Sbjct: 61  SIQTESTSYPSDYSPPMTHLFSSGFSGQDFHGVEQSASIGLNYLTPFQIQQIQSQFGLQT 120

Query: 121 QIQPVWGHVNNQQELTKNQMNGV-NLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVA 180
           QIQPVWG +NNQ  + +NQ N   NLLGPK IPMKH GSPPKSTKLYRGVRQRHWGKWVA
Sbjct: 121 QIQPVWGQINNQ--IARNQSNSAGNLLGPKGIPMKHVGSPPKSTKLYRGVRQRHWGKWVA 180

Query: 181 EIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYK 240
           EIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFP LKHHGSSVGGDFGEYK
Sbjct: 181 EIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPHLKHHGSSVGGDFGEYK 240

Query: 241 PLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQ 300
           PLHSAVDAKL+AICQTLAESQKQGKS+R    RKSKSS  S T GSESQ PTVIDSDD+ 
Sbjct: 241 PLHSAVDAKLEAICQTLAESQKQGKSDR----RKSKSSG-SPTFGSESQTPTVIDSDDEV 300

Query: 301 KASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQK 360
           KA D RSSGSEICCKAE SSSPVS ESDESAGSSPLSDLTFPD TDS WEQ +E  MLQK
Sbjct: 301 KAVDGRSSGSEICCKAEKSSSPVSGESDESAGSSPLSDLTFPDTTDSAWEQATERCMLQK 355

Query: 361 YPSEIDWASIFTS 372
           YPSEIDWASIFTS
Sbjct: 361 YPSEIDWASIFTS 355

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy TrEMBL
Match: A0A345BTG2 (AP2-3 OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 721 bits (1861), Expect = 3.90e-262
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60
           MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS
Sbjct: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60

Query: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120
           SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT
Sbjct: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120

Query: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180
           QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE
Sbjct: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180

Query: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240
           IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP
Sbjct: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240

Query: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300
           LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK
Sbjct: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300

Query: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360
           ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY
Sbjct: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360

Query: 361 PSEIDWASIFTS 372
           PSEIDWASIFTS
Sbjct: 361 PSEIDWASIFTS 372

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy TrEMBL
Match: A0A6J1GHP8 (ethylene-responsive transcription factor RAP2-4-like OS=Cucurbita moschata OX=3662 GN=LOC111454287 PE=4 SV=1)

HSP 1 Score: 713 bits (1840), Expect = 6.67e-259
Identity = 371/374 (99.20%), Postives = 371/374 (99.20%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSS--FLPSFSPSSSSFVPDFSSS 60
           M ALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSS  FLPSFSPSSSSFVPDFSSS
Sbjct: 1   MAALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSSSFLPSFSPSSSSFVPDFSSS 60

Query: 61  FSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGL 120
           FSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGL
Sbjct: 61  FSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGL 120

Query: 121 QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWV 180
           QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWV
Sbjct: 121 QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWV 180

Query: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY 240
           AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY
Sbjct: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY 240

Query: 241 KPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD 300
           KPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD
Sbjct: 241 KPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD 300

Query: 301 QKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQ 360
           QKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQ
Sbjct: 301 QKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQ 360

Query: 361 KYPSEIDWASIFTS 372
           KYPSEIDWASIFTS
Sbjct: 361 KYPSEIDWASIFTS 374

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy TrEMBL
Match: A0A6J1KS42 (ethylene-responsive transcription factor RAP2-4-like OS=Cucurbita maxima OX=3661 GN=LOC111495990 PE=4 SV=1)

HSP 1 Score: 702 bits (1812), Expect = 1.10e-254
Identity = 365/372 (98.12%), Postives = 366/372 (98.39%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60
           M ALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSS FLPSFSPSSSSF PDFSSSFS
Sbjct: 1   MAALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSS-FLPSFSPSSSSFDPDFSSSFS 60

Query: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120
           SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT
Sbjct: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120

Query: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180
           QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKH GSPPKSTKLYRGVRQRHWGKWVAE
Sbjct: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHVGSPPKSTKLYRGVRQRHWGKWVAE 180

Query: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240
           IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP
Sbjct: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240

Query: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300
           LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDD K
Sbjct: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDLK 300

Query: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360
           A +VRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY
Sbjct: 301 AGEVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360

Query: 361 PSEIDWASIFTS 372
           PSEIDWASIFTS
Sbjct: 361 PSEIDWASIFTS 371

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy TrEMBL
Match: A0A6J1KD69 (ethylene-responsive transcription factor RAP2-4-like OS=Cucurbita maxima OX=3661 GN=LOC111492176 PE=4 SV=1)

HSP 1 Score: 553 bits (1426), Expect = 3.17e-196
Identity = 301/372 (80.91%), Postives = 316/372 (84.95%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSFS 60
           M ALMD YGCRD QS PVGGELMEALEPFMKAASSS S             +PDFS S S
Sbjct: 1   MAALMDLYGCRDFQSDPVGGELMEALEPFMKAASSSFS-------------IPDFSFS-S 60

Query: 61  SIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQT 120
           SIQTESTSY SDCSPP+ +     FS+ + HGLEQSASIGLNYLTPFQIQQIQSQF LQT
Sbjct: 61  SIQTESTSYSSDCSPPMIHL----FSSQDFHGLEQSASIGLNYLTPFQIQQIQSQFALQT 120

Query: 121 QIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAE 180
            I PVWG +NNQ  + +NQ NG NLL PK I MK  GSPPKSTKLYRGVRQRHWGKWVAE
Sbjct: 121 LIPPVWGQINNQ--VVRNQRNGGNLLAPKGISMKQVGSPPKSTKLYRGVRQRHWGKWVAE 180

Query: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKP 240
           IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFP LKHHGSSVGGDFGEYKP
Sbjct: 181 IRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPHLKHHGSSVGGDFGEYKP 240

Query: 241 LHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQK 300
           LHSAVDAKL+AICQTLAESQKQGKSNR ASS+KSKSSS SSTV SE + PTVID D++ +
Sbjct: 241 LHSAVDAKLEAICQTLAESQKQGKSNR-ASSKKSKSSS-SSTVESECETPTVIDPDENLR 300

Query: 301 ASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKY 360
             D RSSGSEICCK ENSSSPVSAE+DESAGSSPLSDLTFPDPTDSPWE PSESSMLQKY
Sbjct: 301 TEDARSSGSEICCKVENSSSPVSAENDESAGSSPLSDLTFPDPTDSPWEPPSESSMLQKY 350

Query: 361 PSEIDWASIFTS 372
           PSEIDWASIFTS
Sbjct: 361 PSEIDWASIFTS 350

[Close]

BLAST of Cp4.1LG07g01250 vs. ExPASy TrEMBL
Match: A0A1S3BC06 (ethylene-responsive transcription factor RAP2-4-like OS=Cucumis melo OX=3656 GN=LOC103488407 PE=4 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 1.64e-194
Identity = 302/374 (80.75%), Postives = 319/374 (85.29%), Query Frame = 0


Query: 1   MPALMDFYGCRDLQSYPVGGELMEALEPFMKAASSS--SSSFLPSFSPSSSSFVPDFSSS 60
           M ALMD YGCR+ QS P GGELMEA+EPFMK ASSS   SSF P FS SSSS     SSS
Sbjct: 1   MAALMDLYGCREFQSDPFGGELMEAIEPFMKVASSSYFPSSF-PEFSFSSSS-----SSS 60

Query: 61  FSSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGL 120
            SSI TESTSYPSDCSPP+ + +SDGFS  +  G+EQS SIGLNYLTPFQIQQIQSQFGL
Sbjct: 61  SSSIPTESTSYPSDCSPPMTHLFSDGFSGQDFRGVEQSPSIGLNYLTPFQIQQIQSQFGL 120

Query: 121 QTQIQPVWGHVNNQQELTKNQMNGV-NLLGPKAIPMKHAGSPPKSTKLYRGVRQRHWGKW 180
           QTQIQPVWG +NNQ  + +NQ N   NLLGPK IPMKH GSPPKSTKLYRGVRQRHWGKW
Sbjct: 121 QTQIQPVWGQINNQ--VVRNQSNTAGNLLGPKGIPMKHVGSPPKSTKLYRGVRQRHWGKW 180

Query: 181 VAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGE 240
           VAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFP LKHHGSSVGGDFGE
Sbjct: 181 VAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPHLKHHGSSVGGDFGE 240

Query: 241 YKPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDD 300
           YKPLHSAVDAKL+AICQTLAESQ +GKS+R    RKSKSS  SST+ SE QAPTVID+DD
Sbjct: 241 YKPLHSAVDAKLEAICQTLAESQTKGKSDR----RKSKSSG-SSTIASEPQAPTVIDADD 300

Query: 301 DQKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSML 360
           D K +D  SSGSEICCKAENSSSPVSAESDESAGSSPLSDLTF D TDS WEQ +ES ML
Sbjct: 301 DLKPADAGSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFQDTTDSTWEQATESCML 360

Query: 361 QKYPSEIDWASIFT 371
           QKYPSEIDWASIFT
Sbjct: 361 QKYPSEIDWASIFT 361

[Close]

BLAST of Cp4.1LG07g01250 vs. TAIR 10
Match: AT1G78080.1 (related to AP2 4 )

HSP 1 Score: 265.8 bits (678), Expect = 5.3e-71
Identity = 189/385 (49.09%), Postives = 231/385 (60.00%), Query Frame = 0


Query: 1   MPALMDFYGC-RDLQSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSF 60
           M A M+ Y C R  Q    GGELM+AL PF+K+ S S SS   S + S+S+F+     S 
Sbjct: 1   MAAAMNLYTCSRSFQD--SGGELMDALVPFIKSVSDSPSS---SSAASASAFL---HPSA 60

Query: 61  SSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSAS-IGLNYLTPFQIQQIQSQFGL 120
            S+      YP        +SY           L+Q+ S IGLN L+  QI QIQSQ  +
Sbjct: 61  FSLPPLPGYYPDSTFLTQPFSYGS--------DLQQTGSLIGLNNLSSSQIHQIQSQ--I 120

Query: 121 QTQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKH-----------AGSPPKSTKLYR 180
              + P   H NN    +       NLL PK + MK            +G P K TKLYR
Sbjct: 121 HHPLPPT--HHNNNNSFS-------NLLSPKPLLMKQSGVAGSCFAYGSGVPSKPTKLYR 180

Query: 181 GVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHH 240
           GVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFP L+H+
Sbjct: 181 GVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPNLRHN 240

Query: 241 GSSVGGDFGEYKPLHSAVDAKLDAICQTLAESQKQGKSNRSASSRKSKSSS--ISSTVGS 300
           GS +GGDFGEYKPLHS+VDAKL+AIC+++AE+QKQ KS +S+  R+ K SS  +S  V +
Sbjct: 241 GSHIGGDFGEYKPLHSSVDAKLEAICKSMAETQKQDKSTKSSKKREKKVSSPDLSEKVKA 300

Query: 301 ESQAPTVIDSDDDQKASDVRSSGSEICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTD 360
           E  + ++                          S PV+   + +AGSSPLSDLTF DP +
Sbjct: 301 EENSVSI------------------------GGSPPVTEFEESTAGSSPLSDLTFADPEE 332

Query: 361 SPWEQPSESSMLQKYPS-EIDWASI 370
            P  Q +E+  L+KYPS EIDW SI
Sbjct: 361 PP--QWNETFSLEKYPSYEIDWDSI 332

[Close]

BLAST of Cp4.1LG07g01250 vs. TAIR 10
Match: AT1G22190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 2.1e-51
Identity = 159/374 (42.51%), Postives = 192/374 (51.34%), Query Frame = 0


Query: 1   MPALMDFYGCRDL-QSYPVGGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVPDFSSSF 60
           M   MDFY  +   QS P GGELMEAL PF+K+ S+ SS+F                   
Sbjct: 1   MTTSMDFYSNKTFQQSDPFGGELMEALLPFIKSPSNDSSAF------------------- 60

Query: 61  SSIQTESTSYPSDCSPPVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQ 120
                 + S P+    P++Y        ++LH                            
Sbjct: 61  ------AFSLPA----PISY-------GSDLHSFSHH----------------------- 120

Query: 121 TQIQPVWGHVNNQQELTKNQMNGVNLLGPKAIPMKHAG-SPPKSTKLYRGVRQRHWGKWV 180
                                     L PK + MK  G S  K TKLYRGVRQRHWGKWV
Sbjct: 121 --------------------------LSPKPVSMKQTGTSAAKPTKLYRGVRQRHWGKWV 180

Query: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEY 240
           AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFP L+H+         EY
Sbjct: 181 AEIRLPRNRTRLWLGTFDTAEEAALAYDKAAYKLRGDFARLNFPDLRHN--------DEY 240

Query: 241 KPLHSAVDAKLDAICQTLAE-SQKQGKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDD 300
           +PL S+VDAKL+AICQ LAE +QKQ +S + +SSRK +SS+++  +  E           
Sbjct: 241 QPLQSSVDAKLEAICQNLAETTQKQVRSTKKSSSRK-RSSTVAVKLPEE----------- 253

Query: 301 DQKASDVRSSGSEICCKAENSSSPVSAESDESAG-SSPLSDLTFPDPTDSPWEQPSESSM 360
                D  S+G          SSP+  ES  S G SSPLS+LTF D T+   + P   + 
Sbjct: 301 -----DYSSAG----------SSPLLTESYGSGGSSSPLSELTFGD-TEEEIQPPWNENA 253

Query: 361 LQKYPS-EIDWASI 370
           L+KYPS EIDW SI
Sbjct: 361 LEKYPSYEIDWDSI 253

[Close]

BLAST of Cp4.1LG07g01250 vs. TAIR 10
Match: AT1G36060.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 2.7e-46
Identity = 155/363 (42.70%), Postives = 201/363 (55.37%), Query Frame = 0


Query: 19  GGELMEALEPFMKAASSSSSSFLPSFSPSSSSFVP---DFSSSFSSIQTESTSYPSDCSP 78
           GGELMEAL+PF K+AS+S+S+  P+F+ S+ +F     D  SS S     ++ +PS  + 
Sbjct: 10  GGELMEALQPFYKSASTSASN--PAFASSNDAFASAPNDLFSSSSYYNPHASLFPSHSTT 69

Query: 79  PVAYSYSDGFSANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQTQIQPVWGHVNNQQEL 138
               SY D +S +    +   +S G +   P   +  QSQF  Q  I   + H +N   +
Sbjct: 70  ----SYPDIYSGS----MTYPSSFGSDLQQP---ENYQSQFHYQNTI--TYTHQDNNTCM 129

Query: 139 TKNQMNGVNLLGPK-----AIPMKHAGSPPKSTKLYRGVRQRHWGKWVAEIRLPRNRTRL 198
                  +N + P        P   +GS  K  KLYRGVRQRHWGKWVAEIRLPRNRTRL
Sbjct: 130 -------LNFIEPSQPGFMTQPGPSSGSVSKPAKLYRGVRQRHWGKWVAEIRLPRNRTRL 189

Query: 199 WLGTFDTAEEAALAYDKAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKPLHSAVDAKLD 258
           WLGTFDTAEEAALAYD+AA+KLRGD ARLNFP L++   S   D GEY P+ +AVDAKL+
Sbjct: 190 WLGTFDTAEEAALAYDRAAFKLRGDSARLNFPALRYQTGSSPSDTGEYGPIQAAVDAKLE 249

Query: 259 AICQTLAESQKQ-GKSNRSASSRKSKSSSISSTVGSESQAPTVIDSDDDQKASDVRSSGS 318
           AI   LAE + Q GK+ R  +SRK   ++ SS                +Q ++  + SG 
Sbjct: 250 AI---LAEPKNQPGKTER--TSRKRAKAAASSA---------------EQPSAPQQHSG- 309

Query: 319 EICCKAENSSSPVSAESDESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKYPS-EIDWAS 372
                        S ESD S   SP SD+   +    P    +E+ ML K PS EIDWAS
Sbjct: 310 -------------SGESDGS--GSPTSDVMVQEMCQEPEMPWNENFMLGKCPSYEIDWAS 314

[Close]

BLAST of Cp4.1LG07g01250 vs. TAIR 10
Match: AT4G39780.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.3e-42
Identity = 125/285 (43.86%), Postives = 161/285 (56.49%), Query Frame = 0


Query: 87  ANNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQTQIQPVWGHVNNQQELTKNQMNGVNLL 146
           +N + G  Q+ S+GLN LTP+QI QIQ+Q   +  I            ++ N       L
Sbjct: 37  SNTVFGFNQTTSLGLNQLTPYQIHQIQNQLNQRRNI------------ISPN-------L 96

Query: 147 GPKAIPMKHAGSPPKSTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYD 206
            PK +PMK+      + KLYRGVRQRHWGKWVAEIRLP+NRTRLWLGTFDTAEEAA+AYD
Sbjct: 97  APKPVPMKNM----TAQKLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFDTAEEAAMAYD 156

Query: 207 KAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKPLHSAVDAKLDAICQTLAESQKQGKSN 266
            AAYKLRG+FARLNFPQ +H     GG    + PLHS+VDAKL  ICQ+L          
Sbjct: 157 LAAYKLRGEFARLNFPQFRHEDGYYGGG-SCFNPLHSSVDAKLQEICQSL---------- 216

Query: 267 RSASSRKSKSSSISSTVGSESQAPTVIDSDDDQKASDVRSSGSEICCKAENSSSPVSAES 326
                RK++   +     SE++         + +   +RS         ENS S    + 
Sbjct: 217 -----RKTEDIDLPC---SETELFPPKTEYQESEYGFLRSD--------ENSFS----DE 266

Query: 327 DESAGSSPLSDL-TFPDPTDSPWEQPSESSMLQKYPS-EIDWASI 370
                SSP S + TF D +DS +++   S  L+K+PS EIDW +I
Sbjct: 277 SHVESSSPESGITTFLDFSDSGFDEIG-SFGLEKFPSVEIDWDAI 266

[Close]

BLAST of Cp4.1LG07g01250 vs. TAIR 10
Match: AT2G22200.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 3.8e-37
Identity = 119/287 (41.46%), Postives = 160/287 (55.75%), Query Frame = 0


Query: 88  NNLHGLEQSASIGLNYLTPFQIQQIQSQFGLQTQIQPVWGHVNNQQELTKNQMNGVNLLG 147
           N   G+ +S  +GLN LTP+QI QIQ+Q             +N+++    N       L 
Sbjct: 12  NTSFGVNKSMPLGLNQLTPYQIHQIQNQ-------------LNHRRSTISN-------LS 71

Query: 148 PKAIPMKH-AGSPPKSTKLYRGVRQRHWGKWVAEIRLPRNRTRLWLGTFDTAEEAALAYD 207
           P  I MK+   S  K+  LYRGVRQRHWGKWVAEIRLP+NRTRLWLGTF+TAE+AALAYD
Sbjct: 72  PNRIRMKNLTPSTSKTKNLYRGVRQRHWGKWVAEIRLPKNRTRLWLGTFETAEKAALAYD 131

Query: 208 KAAYKLRGDFARLNFPQLKHHGSSVGGDFGEYKPLHSAVDAKLDAICQTLAESQKQGKSN 267
           +AA++LRGD A+LNFP L H          +  PL S+VD KL AIC++L ++++     
Sbjct: 132 QAAFQLRGDIAKLNFPNLIHE---------DMNPLPSSVDTKLQAICKSLRKTEE----I 191

Query: 268 RSASSRKSKSSSISSTVGSESQAPTVIDSDDDQKASDVRSSGSEICCKAENSSSPVSAES 327
            S S +  + S  S +  +E   P         KA         +     ++ SP S E+
Sbjct: 192 CSVSDQTKEYSVYSVSDKTELFLP---------KAELFLPKREHLETNELSNESPRSDET 251

Query: 328 ---DESAGSSPLSDLTFPDPTDSPWEQPSESSMLQKYPS-EIDWASI 370
              DES      SD TF D +D+ +E+   S  L+K+PS EIDW +I
Sbjct: 252 SLLDESQAEYSSSDKTFLDFSDTEFEEIG-SFGLRKFPSVEIDWDAI 255

The following BLAST results are available for this feature:

BLAST of Cp4.1LG07g01250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H1E4	7.5e-70	49.09	Ethylene-responsive transcription factor RAP2-4 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LM15	3.0e-50	42.51	Ethylene-responsive transcription factor RAP2-13 OS=Arabidopsis thaliana OX=3702...	[more]
Q9SKW5	3.7e-45	42.70	Ethylene-responsive transcription factor ERF055 OS=Arabidopsis thaliana OX=3702 ...	[more]
O65665	3.3e-41	43.86	Ethylene-responsive transcription factor ERF060 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SIE4	5.4e-36	41.46	Ethylene-responsive transcription factor ERF056 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Cp4.1LG07g01250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537287.1	8.05e-262	100.00	ethylene-responsive transcription factor RAP2-4-like [Cucurbita pepo subsp. pepo...	[more]
KAG7020206.1	5.43e-260	99.46	Ethylene-responsive transcription factor RAP2-4, partial [Cucurbita argyrosperma...	[more]
XP_022951481.1	1.38e-258	99.20	ethylene-responsive transcription factor RAP2-4-like [Cucurbita moschata]	[more]
XP_023001968.1	2.28e-254	98.12	ethylene-responsive transcription factor RAP2-4-like [Cucurbita maxima]	[more]
XP_038885142.1	2.03e-198	81.50	ethylene-responsive transcription factor RAP2-4-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG07g01250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A345BTG2	3.90e-262	100.00	AP2-3 OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
A0A6J1GHP8	6.67e-259	99.20	ethylene-responsive transcription factor RAP2-4-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1KS42	1.10e-254	98.12	ethylene-responsive transcription factor RAP2-4-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1KD69	3.17e-196	80.91	ethylene-responsive transcription factor RAP2-4-like OS=Cucurbita maxima OX=3661...	[more]
A0A1S3BC06	1.64e-194	80.75	ethylene-responsive transcription factor RAP2-4-like OS=Cucumis melo OX=3656 GN=...	[more]

BLAST of Cp4.1LG07g01250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78080.1	5.3e-71	49.09	related to AP2 4	[more]
AT1G22190.1	2.1e-51	42.51	Integrase-type DNA-binding superfamily protein	[more]
AT1G36060.1	2.7e-46	42.70	Integrase-type DNA-binding superfamily protein	[more]
AT4G39780.1	2.3e-42	43.86	Integrase-type DNA-binding superfamily protein	[more]
AT2G22200.1	3.8e-37	41.46	Integrase-type DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 166..177 score: 72.73 coord: 188..204 score: 71.66
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 165..228 e-value: 2.1E-37 score: 140.3
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 166..214 e-value: 4.9E-15 score: 55.5
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 165..222 score: 23.169235
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 164..222 e-value: 7.78824E-34 score: 117.758
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 165..223 e-value: 2.0E-27 score: 97.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 258..295
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 258..360
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 311..360
None	No IPR available	PANTHER	PTHR31657	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061	coord: 4..369
None	No IPR available	PANTHER	PTHR31657:SF46	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR RAP2-4	coord: 4..369
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 165..223

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG07g01250.1	Cp4.1LG07g01250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity