Cp4.1LG06g09270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG06g09270 vs. ExPASy Swiss-Prot
Match: Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 676/1517 (44.56%), Postives = 914/1517 (60.25%), Query Frame = 0

Query: 59   LSPMPSLSSCDSDCSTVANLDIKDGQEG-----TTRSSQDSIDCRQERMSQNWSEVVHSN 118
            ++P  SLSS +   S   ++D      G     ++ SSQ+ ++  +E         V   
Sbjct: 1    MTPSNSLSSSERSLSGECSVDGNSCDRGIEDECSSHSSQEDVELTKE-------VKVDRL 60

Query: 119  GQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGM 178
             + S      ++ I  E +   S   V   +     +  D  +W+PPEPE+P+D V+G  
Sbjct: 61   ERKSKSMPSDILDILDEKSKENSVENVQF-LSDREDDSDDVPVWEPPEPENPEDEVDGVF 120

Query: 179  GYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLL 238
                 DDDD+  D ++WN +S      DE+S   ++ E+ +R M +  + KFK  +  L+
Sbjct: 121  A----DDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLI 180

Query: 239  KFVGVASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQF 298
            K  G   S E+S  W +IV+ L WEAA+ LKP ++GK+VDP  ++KVKCIATG+   S+ 
Sbjct: 181  KSAGF--SIEESGYWFEIVARLCWEAASMLKPAIDGKSVDPTEYIKVKCIATGSCVDSEV 240

Query: 299  VKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQEND----FTKHVI 358
             KG+VFKKHAA KHM T  ++P+++L+EG+LG  PIS  SS  S++Q+N+    + K V+
Sbjct: 241  FKGLVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVV 300

Query: 359  EMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMS 418
            ++IE    +V+LVEK+ SR IQ+ +L KG+TLV DMKLHRL+RI+ C GSPIL+ ++L S
Sbjct: 301  DIIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSS 360

Query: 419  QNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIK 478
            Q  + CD+   EKIVEEH    E  K+PTKTLMF+EGCPTRLGCTILLKG HS+ LKK+K
Sbjct: 361  QKLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVK 420

Query: 479  VVVQIAVVMAFHLMLETSFLVDQRAMFATI------------------------PFGGVP 538
             VVQ + ++A+HLMLE SFL D+  MF+TI                        P   V 
Sbjct: 421  EVVQYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVEIENFSPSPSPRESPSEAVD 480

Query: 539  ATVSSDPQSPLEPCSPNAQG------ASNGSNL-EGEPYNPAIFSGLSSISDSLKRVMGE 598
              VS+         +  A G       S+G ++   EPYNP IF+G SS+S  L      
Sbjct: 481  IPVSNGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARL------ 540

Query: 599  SFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQF----DVEVRGSSDEENSIH 658
                       S Y G  ++   +    D+  ST    D      +     + D++  + 
Sbjct: 541  -----------SKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLL 600

Query: 659  EQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSRNALKGTMCEQSHFS 718
            +  +P  S+ D+G        N S+ +   ++  +SQSILVL+S RNAL+G MC+Q HFS
Sbjct: 601  DPELPVNSSSDDG-------DNKSQTENDIESTLESQSILVLVSKRNALRGIMCDQRHFS 660

Query: 719  HIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKV 778
            HI FYK+FD PL KFL++ + NQ+N+C  C E PEAH YYYAH  KQL IQ+K++PV K 
Sbjct: 661  HIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKG 720

Query: 779  LPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSS 838
            L GE +GK+WMWSRC KCK+K    KSTKRVLISTAAR LSFGKFLEL FS  T  ++SS
Sbjct: 721  LAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSS 780

Query: 839  VCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYA 898
             CGHS   DFL+FFGLG+MVAM  Y++VA YTVS+PP KLE +  ++ G L  E + V+ 
Sbjct: 781  SCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFT 840

Query: 899  KGMILFTEIAQFLKKIISERKSSTVND------FSLLEEMLNEERSEFEQNIQSSLTKKG 958
            KG+ LF + A FLK++ S+  +S +         S +EE+L  ER  FE+NI++S  K  
Sbjct: 841  KGISLFEDAAGFLKRLRSQFTNSDLRYQRARKLLSNIEELLKHERCIFEENIKNSFDKAK 900

Query: 959  NPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEPVGHEGLES 1018
              D   H+ L LNR+ WELLL++ IW+ RLQ L      L+     + +   +  +GL++
Sbjct: 901  TIDDVSHRLLRLNRMRWELLLQALIWNYRLQSL-----VLSDRLLPSSDETKIYEQGLKT 960

Query: 1019 IAQTDDRNVQQDVSVDENALHP--KEIAVEHLD------GESDGDELGLPSATEVTEIPI 1078
            +++      + D  V ++  +       VEH D         D D++      E  E   
Sbjct: 961  VSEAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGASVGDNDQMAESYVPEDNESQT 1020

Query: 1079 MDDLSPKQLSRQGSLSNGF-----------NRRHSDDEDPQAGRVLSSGDIPSSTGN--L 1138
            +   SP   S    ++N F           N + +D   P  G  L   ++ +S G   L
Sbjct: 1021 LCSSSPDTTS---PINNHFDTHLAVNVHSTNGQEADKSIPVTGESLDD-EVSTSNGPHIL 1080

Query: 1139 TLDKLFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSN 1198
              D+ FW PF E++  R+ DI++ Y  + + ++N+T + L T +  I +E  +L I L +
Sbjct: 1081 GWDEWFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQIITEESSRLRISLRD 1140

Query: 1199 EKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSN--GSSDSDSSVS 1258
            + FIVSDYE ELSS++AC LA          L+NE+SK      +  +  G  D++    
Sbjct: 1141 DDFIVSDYEDELSSLIACALA---------HLNNEESKKPLSRCIHGSLQGFLDNNQDSK 1200

Query: 1259 SEEHQFSSF--DRLNLLDSLLPK----TFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLR 1318
              +   S F  +  N L++L P     TF      G +KS+ K KY +   Y + FRDLR
Sbjct: 1201 QTDRDVSRFSSESTNRLETLPPPEVLVTF------GSVKSVGKPKYSIVSLYADDFRDLR 1260

Query: 1319 RRCCPSELHYIASLSRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYF 1378
            +RCC SEL YIASLSRC  W+AKGGKSKS FAKTLDDRFI+KEIK+TEYESF+ FA +YF
Sbjct: 1261 KRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYF 1320

Query: 1379 KYINESFDMGNQSCLAKVLGIYQVTVRDPK-SGKEVRHELMVMENLSFGRNIIRQYDLKG 1438
            KY+ +S+D+GNQ+CLAKVLGI+QVTVR PK  GKE+RH+LMVMENLSF R + RQYDLKG
Sbjct: 1321 KYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKG 1380

Query: 1439 ALHARYN-PATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMD 1494
            ALHAR+   + N   +VLLDQNFVNDMN SPLYV+  +K+ LQRA++NDT+FL S+NVMD
Sbjct: 1381 ALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINVMD 1440

BLAST of Cp4.1LG06g09270 vs. ExPASy Swiss-Prot
Match: Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.0e-217
Identity = 539/1477 (36.49%), Postives = 771/1477 (52.20%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDDEFDDSTEW---NTSSSFSRSVDEASVSYRIKEQ 215
            LW PPEPE+ +D  E  +    D++D+E D S EW     S+SF     E     R  E+
Sbjct: 318  LWVPPEPENEEDERESAL---FDEEDNEGDASGEWGYLRPSTSFGSG--EYRGEDRTTEE 377

Query: 216  KQRAMQQIMNGKFKVFIGHLLKFVGVASSGED-SENWVDIVSSLSWEAATFLKP-VVNGK 275
             ++AM+ +++G F+  +  LL+   ++ S E+  E+W++I++SLSWEAA  LKP +    
Sbjct: 378  HKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLLKPDMSKSG 437

Query: 276  AVDPEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS 335
             +DP  +VKVKC+A+G R  S  VKG+V KK+  ++ M T  +  +L+++ G L    +S
Sbjct: 438  GMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGGGLEYQRVS 497

Query: 336  -RLSSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLH 395
             +LSSF++ + QE D  K  +  I     N++LVEK+ SR  QE +L K ++LVL++K  
Sbjct: 498  NQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDISLVLNIKRP 557

Query: 396  RLERIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGC 455
             L+RIA CTG+ I+ S + L SQ    C+    ++  EEH    + GK+  KTLM+ E C
Sbjct: 558  LLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGKKVVKTLMYFEHC 617

Query: 456  PTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRA---------MFAT 515
            P  LG TILL+GA+ DELKK+K VVQ  V  A+HL LETSFL D+ A         +   
Sbjct: 618  PKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGASPELPLNSPITVA 677

Query: 516  IP---------FGGVPA-TVSSDPQSP--LEPCSPNAQGASNGSNLEGEPYNPAIFSGLS 575
            +P            VP  TVS+  +SP  L    P    +   S L     N +I   + 
Sbjct: 678  LPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELLSTTTNLSIQKDIP 737

Query: 576  SI---SDSLKRVMGESFFLASPCQSFS----SYFGHGKDLSGLVAKSDSIPSTPPGADQF 635
             I   S    R +  SF  +    S +           DLSG      S+P   P AD+ 
Sbjct: 738  PIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSG-----RSVPVDTP-ADKS 797

Query: 636  DVEVRGSSDEENSIH---EQFVPHQSTF-------DEGFGFHETAPNYSEDKLQKKT--- 695
            +  V       NS+H   + FV   S         ++  G   T      ++  K+T   
Sbjct: 798  NPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNEKPKETQSQ 857

Query: 696  -------PFDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKN 755
                   P D QSILV +SSR+  KGT+CE+SH   I +Y +FD PLG+FL+++L +Q  
Sbjct: 858  KEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRDHLFDQSY 917

Query: 756  VCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPS 815
             C  C    EAH + Y H +  L I VK+L  + +LPGE EGK+WMW RC +C    G  
Sbjct: 918  RCRSCEMPSEAHVHCYTHRQGSLTISVKKLQ-DYLLPGEKEGKIWMWHRCLRCPRLNGFP 977

Query: 816  KSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRY 875
             +T RV++S AA GLSFGKFLEL FS+    S+ + CGHSL  D L F+G GNMVA FRY
Sbjct: 978  PATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFRY 1037

Query: 876  TEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTV 935
              + +++V +PP  L FN    Q  +  ET+ V  +  +LF+E+   + +I  +     +
Sbjct: 1038 ATIDVHSVYLPPSILSFNYE-NQDWIQRETDEVIERAELLFSEVLNAISQIAEKGFRRRI 1097

Query: 936  NDFSLLEEMLNEERSEFEQNIQSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQ 995
             +   LEE+L +E++EFE+N+Q  L ++ N   P    L L R+  +LL +S +WD RL 
Sbjct: 1098 GE---LEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLI 1157

Query: 996  LLTSVGVTLNSGTSENVEPE----------PVGHEGLESIAQTDDRNVQQ------DVSV 1055
              +++    NS  ++  E E          P  + G  S+    + N+        D   
Sbjct: 1158 NASTLHKLENSDDTKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGS 1217

Query: 1056 DENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDLSPKQLSRQGSL-------- 1115
              N     +   +    + DG E+        T  P+ + +  ++    G +        
Sbjct: 1218 LNNVQKEADTNSDLYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSAT 1277

Query: 1116 --SNGFNRRHSDDEDPQAGRV---------------LSSG-------------------- 1175
              +     R +  E P   +V               +S G                    
Sbjct: 1278 LDAAWIGERQTSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVS 1337

Query: 1176 -DIPSSTGNLTLDKLFW--TPFSEIQQMRMRDIQRSYF---PELKSISNYTPKLLPTAHD 1235
              +PS     + D + W   PF        R I +++     +L +   ++P  + +  +
Sbjct: 1338 PALPSKNYENSEDSVSWLSVPFLNF----YRSINKNFLLSSQKLDTFGEHSPIYISSFRE 1397

Query: 1236 FINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKV 1295
               + G +L + +     +V  Y+ E +S++A  L     +S E       ++GE L   
Sbjct: 1398 AELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYAL-----MSPEYQ-RQTSAEGESLVSY 1457

Query: 1296 PSN------------GSSDSDSSVSSEEHQFSSFDRLNLLDSLLPKTFKRAEHE----GI 1355
            PS               S S+ SV       SS    +LLD   P ++ +A H     G 
Sbjct: 1458 PSELNIPRPVDDTIFDPSRSNGSVDESILSISSSRSTSLLD---PLSYTKALHARVSYGE 1517

Query: 1356 IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWNAKGGKSKSFFAKTLDD 1415
              +L K KY V C Y  +F  LR  C PSEL YI SLSRC  W A+GGKS  FFAKTLDD
Sbjct: 1518 DGTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDD 1577

Query: 1416 RFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGIYQVTVRDPKSGKEVRH 1475
            RFIIK++ +TE ESF+KFAP YFKY++ES    + +CLAK+LGIYQV  +  KSGKE + 
Sbjct: 1578 RFIIKQVTKTELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKM 1637

Query: 1476 ELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAK 1491
            ++++MENL FGR + R YDLKG+  ARYNP ++   +VLLDQN +  M +SP++V N+AK
Sbjct: 1638 DVLIMENLLFGRTVKRLYDLKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAK 1697

BLAST of Cp4.1LG06g09270 vs. ExPASy Swiss-Prot
Match: Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.2e-211
Identity = 505/1430 (35.31%), Postives = 774/1430 (54.13%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQR 215
            +W PP PED  D  E    + DD+DDD  D +TE++ SSSFS  +       ++ E    
Sbjct: 263  IWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHI---PTKEKLGENSNE 322

Query: 216  AMQQIMNGKFKVFIGHLLKFVGVASSGEDSE-NWVDIVSSLSWEAATFLKPVVN-GKAVD 275
             ++ +++  F+  +  LL+   ++ S + S   W+DIV++L+W+AA F+KP    G ++D
Sbjct: 323  PLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPDTRAGGSMD 382

Query: 276  PEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RL 335
            P  +VK+KC+A+G +++S  ++G+V  K+  HK M +  KNP+++L+ G L    ++ +L
Sbjct: 383  PGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQL 442

Query: 336  SSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLE 395
            +SFN+ + QEN+  K +I  IE    NV+LVEK+ S   Q+ +L+K ++LVL++K   L+
Sbjct: 443  ASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLLD 502

Query: 396  RIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTR 455
            RIA CTG+ +  S +++ +     C+    E+++E+H    +  ++P++TLM+ EGCP R
Sbjct: 503  RIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGNQSNRKPSRTLMYFEGCPRR 562

Query: 456  LGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPF--GGVPATV 515
            LGCT++L+G+  +ELKK+K V+Q AV  A+HL LETSFL D+ A    I     G+  T 
Sbjct: 563  LGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRLKQPGMVRTA 622

Query: 516  S------------------SDPQSPLEPCSPNAQGASNGSNLE-----GEPYNPA-IFSG 575
            S                  +D Q+ LE  +   +  +     E      E ++P  IF  
Sbjct: 623  SQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPEHEVCESLCEDFDPTQIFPP 682

Query: 576  LSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDL-SGLVAKSDSIPSTPPGADQFDVEV 635
             S +       +   F      +S+SS      DL    +  S  IP TP      + E 
Sbjct: 683  SSEVETEQSDTLNGDFANNLVTRSYSS--NQLNDLHEPTLCLSSEIPETPTQQPSGE-ED 742

Query: 636  RGSSDEENS-IHEQFVPHQSTFDEGFGFHETAPNYSEDKLQK--KTPFDSQSILVLMSSR 695
             G  +EEN  ++ Q +P   +F            Y +D   +        QSILV  SSR
Sbjct: 743  NGRGEEENQLVNPQDLPQHESF------------YEDDVSSEYFSAADSHQSILVSFSSR 802

Query: 696  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 755
              LK ++CE+S    I FY +FD PLG++L+++L ++ + C  C EL +AH   Y+H   
Sbjct: 803  CVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVLCYSHQNG 862

Query: 756  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 815
             L I V++LP  K LPGE +GK+WMW RC +C    G   +T+RV++S AA GLSFGKFL
Sbjct: 863  NLTINVRRLPSMK-LPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWGLSFGKFL 922

Query: 816  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 875
            EL FS+    ++ + CGHSL  D L F+G GNMVA FRY+ + I TV +PP  LEFNS  
Sbjct: 923  ELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSMLEFNSHP 982

Query: 876  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVND------------FSLLEEM 935
            +Q  + TE   +  K   ++TEI+  L ++  E KSS +                L++++
Sbjct: 983  QQEWIRTEAAELVGKMRTMYTEISDMLNRM--EEKSSLLEPEQSEACDLHSRIIGLIDQL 1042

Query: 936  LNEERSEFEQNIQ----SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLL--- 995
            + +E+ E++  +Q     +L  +G+ D      L LNRL   L++ +  WD +L LL   
Sbjct: 1043 V-KEKDEYDDALQPIFEENLQIQGSLDI-----LELNRLRRALMIGAHAWDHQLYLLNSQ 1102

Query: 996  ---TSVGVTLNSGTSENVE---PEPVGHEGLESIAQTD-----DRNVQQDVSVDENALHP 1055
                SV  T +     N E   P  +     E   + D     D     D    EN   P
Sbjct: 1103 LKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPSP 1162

Query: 1056 KEIAVEHLDGESDGDELGLPSATEVTEIPIMD--DLSPKQLSRQGSLSNGFNRRHSDDED 1115
                 E +D    G    L  A  + E       + S ++L+R         R  S D  
Sbjct: 1163 GTSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARP-------IRVQSFDSA 1222

Query: 1116 PQAGRVLSSGDIPSSTGNLTLDKLFWT-PFSEIQQMRMRDIQRSYFPELKSISNYTPKLL 1175
             +    +  G  PSS    TL     +  +  + +  + ++ R+Y   L         ++
Sbjct: 1223 IRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDLIV 1282

Query: 1176 PTAHDFINK-----EGQKLHIFLSN-EKFIVSDYEGELSSIVACVLALLKDLSLETDLHN 1235
             +A  +I+      +G ++ I        +V  Y+ + +S+V+              +++
Sbjct: 1283 GSAPTYISSASQMADGARMLIPQRGLNDIVVPVYDDDPASVVSYA------------INS 1342

Query: 1236 EDSKGEGLNK-VPSNGSSDSDSSVSSEEHQFSSFDRLNL-LDSL---------------- 1295
            ++ K   +NK + S+ SS + ++  SE   FS++  L++ +D +                
Sbjct: 1343 KEYKEWIVNKGLASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKSPH 1402

Query: 1296 LPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWNAKG 1355
            L  +F          +  K K+ V C +  QF  LR+ CCPSE+ ++ SLSRC  W+A+G
Sbjct: 1403 LTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQG 1462

Query: 1356 GKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGIYQV 1415
            GKS  +FAK+LD+RFIIK++ +TE +SF  FAP+YFKY+ ES   G+ +CLAK+LGIYQV
Sbjct: 1463 GKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQV 1522

Query: 1416 TVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFVND 1475
            +++ PK GKE + +LMVMENL + R I R YDLKG+  +RYNP T+   +VLLD N +  
Sbjct: 1523 SIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLET 1582

Query: 1476 MNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLRQYT 1491
            + + P+++ ++AKR L+RAIWNDT FL S++VMDYSLLVG D E+KELV GIID++RQYT
Sbjct: 1583 LRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQYT 1642

BLAST of Cp4.1LG06g09270 vs. ExPASy Swiss-Prot
Match: Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 2.7e-211
Identity = 520/1435 (36.24%), Postives = 748/1435 (52.13%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQR 215
            LW PPEPE+ +D  E  +  DDD D+ +  D      S+SF+         +  K++   
Sbjct: 311  LWLPPEPENEEDEREAVLS-DDDGDEGDRGDWGYLRPSNSFNEK------DFHSKDKSSG 370

Query: 216  AMQQIMNGKFKVFIGHLLKFVGVASSGE-DSENWVDIVSSLSWEAATFLKP-VVNGKAVD 275
            AM+ ++ G F+  +  LL+   +    E D E W+DI++SLSWEAAT LKP       +D
Sbjct: 371  AMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMD 430

Query: 276  PEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RL 335
            P  +VKVKCI  G RS+S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +L
Sbjct: 431  PGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQL 490

Query: 336  SSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLE 395
            SSF++ + QE D  K  +  I+    +++LVEK+ SR  QE +L K ++LVL++K   LE
Sbjct: 491  SSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLE 550

Query: 396  RIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTR 455
            RI+ CTG+ I+ S + L S     CD  + EK VE H    +  K+  KTLMF +GCP  
Sbjct: 551  RISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKP 610

Query: 456  LGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSS 515
            LGCTILLKGAH DELKK+K V+Q  V  A+HL LETSFL D+ A    +P    P TV+ 
Sbjct: 611  LGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVAL 670

Query: 516  DPQ--------SPLEPCSPNAQGASNGSNLEGEPY--NPAIFSGLSSISDSLK-RVMGES 575
              +        S +   + ++   S  + L GEP+  N  +    +S     + ++ G  
Sbjct: 671  PDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGND 730

Query: 576  FFLASPCQSFSSYFGHGKDLSGLVAKSDS-IPSTPPGADQFDVEVRGSSDEENSI----H 635
                S     +    + K    + +K D  +P+       F VE      ++ S+     
Sbjct: 731  RIDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGAT 790

Query: 636  EQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPF-----DSQSILVLMSSRNALKGTMCE 695
            EQ      T D     ++   N  E     K  F     D QSILV +S+R   KG++CE
Sbjct: 791  EQVTDGGYTNDSAVIGNQNF-NRQEQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCE 850

Query: 696  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 755
            ++H   I +Y +FD PLG+FL++NL +Q   C  C    EAH + Y H +  L I VK+L
Sbjct: 851  RAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKL 910

Query: 756  PVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 815
            P  ++LPG+ EGK+WMW RC KC    G   +T+R+++S AA GLSFGKFLEL FS+   
Sbjct: 911  P--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAA 970

Query: 816  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITET 875
             S+ + CGHSL  D L F+G G MVA FRY  + IY V++PP KL FN    Q  L  E+
Sbjct: 971  ASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYE-NQEWLQKES 1030

Query: 876  ENVYAKGMILFTEIAQFLKKIISE-----RKSSTVNDFSL-LEE---MLNEERSEFEQNI 935
            + V  K  +LF E+ + L +I ++      K ST N   L LEE   +L + + E++ ++
Sbjct: 1031 KEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSL 1090

Query: 936  QSSL--TKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEP 995
            Q  L   K G P       L +N+L   ++ +S  WD+ L    ++         +N  P
Sbjct: 1091 QQMLNVVKDGQPTIDI---LLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAP 1150

Query: 996  EPVGHE-GLESIAQTDDRNVQQDVSVDENAL----------------------------- 1055
            + +G    LE ++    +++   V++  ++L                             
Sbjct: 1151 KVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDV 1210

Query: 1056 ---HPKEIAVEHLDGESDGD------ELGLPSATE--VTEIPIMDDLSPKQLSRQGSLSN 1115
                P +  +E    E   D      ++  P+ TE       + D L    +  Q +  N
Sbjct: 1211 GSDRPPDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSEN 1270

Query: 1116 GFNR---RHSDDEDPQAG--RVLSSGDIPSSTGNLTLDKLFWTPFSEIQQMRMRDIQRSY 1175
            G  R   R +     Q    R+L S    +  G  T D+   T            + ++Y
Sbjct: 1271 GIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDE-HTTQVQLPSPSFYYSLNKNY 1330

Query: 1176 FPELKS--ISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALL 1235
                +   ++   P  + +  +   + G +L + L     ++  Y+ E +SI+A  L   
Sbjct: 1331 SLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALT-- 1390

Query: 1236 KDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLLDSLLPKTFKR 1295
                 +  +   D   + L+   S    DS + +S       S D    L S   +  + 
Sbjct: 1391 -SSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQL 1450

Query: 1296 AEHEGIIKSL------------AKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIH 1355
                  +K L             K KY V C Y  +F  LR  CCPSE  +I SL RC  
Sbjct: 1451 LHSSLYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRK 1510

Query: 1356 WNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVL 1415
            W A+GGKS  FFAK+LDDRFIIK++ +TE ESF+KF P YFKY+ ES    + + LAK+L
Sbjct: 1511 WGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKIL 1570

Query: 1416 GIYQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQ 1475
            GIYQV+ +  K GKE + +++VMENL F RN  R YDLKG+  ARYNP T+    VLLDQ
Sbjct: 1571 GIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQ 1630

Query: 1476 NFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDY 1491
            N V  M +SP++V ++AKR L+RA+WNDT+FL S++VMDYSLLVGVD E+ ELV GIID+
Sbjct: 1631 NLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDF 1690

BLAST of Cp4.1LG06g09270 vs. ExPASy Swiss-Prot
Match: O59722 (1-phosphatidylinositol 3-phosphate 5-kinase fab1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fab1 PE=1 SV=2)

HSP 1 Score: 332.8 bits (852), Expect = 2.0e-89
Identity = 376/1488 (25.27%), Postives = 628/1488 (42.20%), Query Frame = 0

Query: 228  FIGHLLKFVGVASSGEDSE-NWVDIVSSLSWEAATFLKP-VVNGKAVDPEAHVKVKCIAT 287
            F+G LL F  +   G DS   WV  +  +  + A  L P + +G  +D  ++VK+K I  
Sbjct: 474  FLGKLL-FQMLNDEGVDSPMQWVLCLPKILLKMALDLGPDIRSGDDIDVRSYVKIKKIPG 533

Query: 288  GTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISR--LSSFNSMDQEND 347
            G+      V G++F K A+ K M    + P++ L+   L  A   +  LS    + Q+ +
Sbjct: 534  GSIQDCFLVNGVLFSKKASSKSMDRSLRRPRIALLTFSLDYACDEQRILSLDLIISQQEE 593

Query: 348  FTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILT 407
            +  +++  I +   N++  +     +  +   + G+     +K   L  IA C  + I++
Sbjct: 594  YIINLVNRICMLKPNLVFAQGQIPSIALKYFEEHGVIAFHGLKESVLYDIARCCRADIIS 653

Query: 408  SETLMSQNTRQCDAVYFE---KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGA 467
            S   +S   R      F+    +V+E+ GL        KT   ++ C  RLGCTI+L+GA
Sbjct: 654  SIDKLSLCPRLGTCGRFQLRTYVVDENKGL-------RKTFAILDRCSERLGCTIVLRGA 713

Query: 468  HSDELKKIKVVVQIAVVMAFHLMLETSFLVDQ--------RAMFATIPFGGVPA------ 527
              ++L K+K +V++ V++A+H+ LE + L D+           + ++    +P+      
Sbjct: 714  DYNQLSKVKKIVELVVLIAYHIKLECALLRDKFVNMPELFETTYQSLSRKSLPSFASTAA 773

Query: 528  -----------TVSSDPQSPLEPCSPNAQGASN--GSNLEGEPYN--PAIFSGLSSISDS 587
                       +++SD QS L P     Q  S+  GSN   E  N  PA     SSI+ +
Sbjct: 774  DKEKSQNHEKKSLNSDNQS-LRPLENENQSVSSTQGSNSPLELINNLPA-SDDYSSITKA 833

Query: 588  LK-RVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSI-PSTPPGADQFDVEVRGSSDE 647
            LK R +  S FL+ P     +   + + +   + K   + P +P G+  F ++   + + 
Sbjct: 834  LKTRFLTFSPFLSKPLPRLLNQVNYYQFIRNKLLKDVKLHPYSPTGS--FVMKQSENDNV 893

Query: 648  ENSIHEQFVPHQSTFDEGFGFHET--APNYSEDKLQKKTPFDSQSILVLMSSRNALKGTM 707
            E S  E +       DE + F E      YS  KL   +PF SQ I++L S  N      
Sbjct: 894  EESYEESY--KFFCIDERYHFLEKQWTLYYSHSKLM-FSPFSSQRIILLYSIINKETSVP 953

Query: 708  CEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEA-----HYYYYAHSRKQL 767
            C       + FY+  D  LG++++++ LN    C   GE  +      HY  Y H   ++
Sbjct: 954  CIGPERCLLEFYRETDCTLGQYIEDSCLNTNVSCG--GEYCKTNDMLWHYRSYVHGNSRI 1013

Query: 768  CIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLEL 827
             + ++    +  +PG  E K+ MWS C  CK      K+T   ++S      SFGK+LE 
Sbjct: 1014 SVFLESF--SCPVPG-LEEKIIMWSYCKFCK------KNTHITVMSEETWKYSFGKYLEF 1073

Query: 828  CFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQ 887
             F +  +  +   C HS+    +++FG  NM   F+   + I+ + +P   L  N S  +
Sbjct: 1074 MFYNSQIRDRFEFCDHSVMAQHVHYFGYCNMALRFQRDLIEIFELFVPSVTLRNNPSYIK 1133

Query: 888  GHLITETENVYAKGMI--LFTEIAQFLKKI------ISERKSSTVNDFSLLEEMLNEERS 947
               + E E    KG+I    + +A  + +I        E+  S  ++ S    +L+ + +
Sbjct: 1134 E--LKEKEYKRLKGVIEKCLSSVASRINQIKCDWVTDPEKFESCTSEISKFRTLLSSDYT 1193

Query: 948  EFEQNIQSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRL---------------- 1007
            E      S        D     YLSLN +L  L  +   W++R                 
Sbjct: 1194 ELYSEFDSIYLNSSTSD-----YLSLNSILRVLQGKMVKWEQRFLDYQRLYLPSYKELSK 1253

Query: 1008 ------------QLLTSVGVTLNSGTSENVEPEPVGHEGLESIAQTDDRNVQQDVSVDEN 1067
                        + L+   + L   T EN + +       ES     ++  Q +V+ ++ 
Sbjct: 1254 IAAAQIKKVFLERPLSQTPLDLPE-TLENTQIDIYPSFKTESTDDQLEKVTQTNVASNKR 1313

Query: 1068 ALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDLSPKQLSRQGSLSN-GFNRRHSD 1127
                 +        ESD   +   S      I   +D+S +       +SN GF+R+   
Sbjct: 1314 VAPYADSMANVGSPESDCFSVATSSDIPKANIDFTNDISTQNTFPASPVSNSGFSRQTYP 1373

Query: 1128 DEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEIQQMRMRDIQRSYFPELKSISN---Y 1187
            +   + G  + S    S++   T D+ F    S    M M  I  S   ++ SI N   Y
Sbjct: 1374 NISQRQGVNMLSHKRKSAS---TSDRRF-VNASSTSGMNM-PISSSISAKISSIQNSTKY 1433

Query: 1188 TPKLLPTAHDF----INKEGQKLHIFLS-----NEKFIVSDYEGELSSIVA-CVLALLKD 1247
            +P+    A D     + +  ++L + L      +E+ I +  +  L  + +  V+ +  D
Sbjct: 1434 SPRKPIPAKDVRVSSLVRRFEELSLQLQEKQKRDEELIKARRKRALPVVPSKPVVEVFND 1493

Query: 1248 LSLETDLHN-EDSKG-----------------EGLNKVPSNGSSDSDSSVSS-------- 1307
            L+   D  N ED  G                 + ++K   N SS+ D+S  +        
Sbjct: 1494 LNEAFDDENSEDENGINDTKENRATESNFSGVDSMSKERENVSSNEDNSPEAFEDIFGIL 1553

Query: 1308 --------EEHQFSSFDRLNLLDSLLPKTFKRAEHEGIIKSLA----------------- 1367
                    E+       +++   S LP +   A+   + + L+                 
Sbjct: 1554 FKNESGLEEQQNLEPSSQMDKEGSKLPTSGPLADKTSVYRILSAFWNEWNSLNPPPFEFP 1613

Query: 1368 ------------------------------------------------------------ 1427
                                                                        
Sbjct: 1614 LQPTEHMFSDSNVIIREDEPSSLISFTLSSPDYLSKMVEIEDSMDEALTNQGLQGSTQFK 1673

Query: 1428 ------------------KGKYLVNCPYF--NQFRDLRRRCCPSELHYIASLSRCIHWNA 1487
                              +G   ++C  F   QF  L RR C  E  ++ SL+RC  W +
Sbjct: 1674 IENLMLKPTGTHLKYQFEEGSARLSCKVFFAEQFSAL-RRACGCEETFVTSLARCSLWES 1733

Query: 1488 KGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGIY 1489
             GGKS S F KT D ++I+K + R E +S + FAP YF YI++ F     + L K+ G Y
Sbjct: 1734 SGGKSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALTKIFGFY 1793

BLAST of Cp4.1LG06g09270 vs. NCBI nr
Match: XP_023535373.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita pepo subsp. pepo] >XP_023535374.1 putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3037 bits (7874), Expect = 0.0
Identity = 1522/1522 (100.00%), Postives = 1522/1522 (100.00%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900
            RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260
            DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN
Sbjct: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320

Query: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGITDRASSQTNAEENDLSS 1522
            GACGITDRASSQTNAEENDLSS
Sbjct: 1501 GACGITDRASSQTNAEENDLSS 1522

BLAST of Cp4.1LG06g09270 vs. NCBI nr
Match: KAG7025096.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3004 bits (7789), Expect = 0.0
Identity = 1507/1523 (98.95%), Postives = 1514/1523 (99.41%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQEL+TMNLDNKSSSE LMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGAS GSNLEGEPYNPA
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASYGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPP ADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKL +KTPF+SQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NA KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900
            RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFE NI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSLSNGFN RHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNHRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260
            DSLLPKTFKRAEHEG+IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQ+CLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGIT-DRASSQTNAEENDLSS 1522
            GACG T DRASSQTNAEENDLSS
Sbjct: 1501 GACGGTIDRASSQTNAEENDLSS 1523

BLAST of Cp4.1LG06g09270 vs. NCBI nr
Match: KAG6592249.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2993 bits (7760), Expect = 0.0
Identity = 1502/1523 (98.62%), Postives = 1511/1523 (99.21%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQEL+TMNLDNKSSSE LMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSID RQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDGRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHS+WLDTDLWDPPEPEDPQDHVEGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSDWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPP ADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKL +KTPF+SQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NA KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900
            RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFE NI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            VGH GLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLP ATEVTEIPI
Sbjct: 961  VGHGGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPLATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSLSNGFN RHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNHRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260
            DSLLPKTFKRAEHEG+IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQ+CLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVR+PKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GAC-GITDRASSQTNAEENDLSS 1522
            GAC G  DRASSQTNAEENDLSS
Sbjct: 1501 GACDGTIDRASSQTNAEENDLSS 1523

BLAST of Cp4.1LG06g09270 vs. NCBI nr
Match: XP_022932526.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita moschata])

HSP 1 Score: 2992 bits (7758), Expect = 0.0
Identity = 1502/1523 (98.62%), Postives = 1513/1523 (99.34%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCIC+VCKENQEL+TMNLDNKSSSE LMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDH+EGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHMEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            D EFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  D-EFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPP ADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKL +KTPF+SQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NA KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900
            RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFE NI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            VG EGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VGQEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQA RVLSSGDIPSSTGNLTLDKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAVRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKS+SNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSVSNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260
            DSLLPKTFKRAEHEG+IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQ+CLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVR+PKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGIT-DRASSQTNAEENDLSS 1522
            GACG T DRASSQTNAEENDLSS
Sbjct: 1501 GACGGTIDRASSQTNAEENDLSS 1522

BLAST of Cp4.1LG06g09270 vs. NCBI nr
Match: XP_022976897.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita maxima] >XP_022976898.1 putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita maxima])

HSP 1 Score: 2971 bits (7702), Expect = 0.0
Identity = 1492/1523 (97.96%), Postives = 1507/1523 (98.95%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCIC+VCKENQELE MNLDNKSSSE LMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            D EFDDSTEWNTSSSFSRSVDEAS+SYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  D-EFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGE SENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYN A
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPP ADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKL KKTPF+SQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPV+KVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFN+SM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSM 840

Query: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900
            RQGHLITETENVY KGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFE NI
Sbjct: 841  RQGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS+GVT+NSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEP 960

Query: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            V HEGLESIAQTDDRNVQQDVSVDENALH KEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VRHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSL NGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLT DKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAH+FINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPS+GSSDSDSSV+SEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260
            DSLLPKTFKRAEHEG+IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCI+WN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQ+CLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVR+PKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA+KKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGIT-DRASSQTNAEENDLSS 1522
            GACG T D ASSQTNAEENDLSS
Sbjct: 1501 GACGGTIDHASSQTNAEENDLSS 1522

BLAST of Cp4.1LG06g09270 vs. ExPASy TrEMBL
Match: A0A6J1EWK7 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111439025 PE=4 SV=1)

HSP 1 Score: 2992 bits (7758), Expect = 0.0
Identity = 1502/1523 (98.62%), Postives = 1513/1523 (99.34%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCIC+VCKENQEL+TMNLDNKSSSE LMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLD+KDGQ+GTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDH+EGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHMEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            D EFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  D-EFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPP ADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKL +KTPF+SQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPEKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NA KGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NASKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840

Query: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900
            RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFE NI
Sbjct: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960

Query: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            VG EGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VGQEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQA RVLSSGDIPSSTGNLTLDKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAVRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKS+SNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSVSNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260
            DSLLPKTFKRAEHEG+IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQ+CLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVR+PKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGIT-DRASSQTNAEENDLSS 1522
            GACG T DRASSQTNAEENDLSS
Sbjct: 1501 GACGGTIDRASSQTNAEENDLSS 1522

BLAST of Cp4.1LG06g09270 vs. ExPASy TrEMBL
Match: A0A6J1IGZ0 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111477135 PE=4 SV=1)

HSP 1 Score: 2971 bits (7702), Expect = 0.0
Identity = 1492/1523 (97.96%), Postives = 1507/1523 (98.95%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCIC+VCKENQELE MNLDNKSSSE LMLS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180
            DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240
            D EFDDSTEWNTSSSFSRSVDEAS+SYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS
Sbjct: 181  D-EFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300
            SGE SENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK
Sbjct: 241  SGEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360
            KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420
            LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480
            EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNPA 540
            HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYN A
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNAQGASNGSNLEGEPYNAA 540

Query: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFD 600
            IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPP ADQFD
Sbjct: 541  IFSGLSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPEADQFD 600

Query: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSR 660
            VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKL KKTPF+SQSILVLMSSR
Sbjct: 601  VEVRGSSDEENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLPKKTPFNSQSILVLMSSR 660

Query: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720
            NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK
Sbjct: 661  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 720

Query: 721  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780
            QLCIQVKQLPV+KVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL
Sbjct: 721  QLCIQVKQLPVDKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 780

Query: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 840
            ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFN+SM
Sbjct: 781  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSM 840

Query: 841  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFEQNI 900
            RQGHLITETENVY KGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFE NI
Sbjct: 841  RQGHLITETENVYEKGMILFTEIAQFLKKIISERKSSTVNDFSLLEEMLNEERSEFELNI 900

Query: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP 960
            QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTS+GVT+NSGTSENVEPEP
Sbjct: 901  QSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEP 960

Query: 961  VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020
            V HEGLESIAQTDDRNVQQDVSVDENALH KEIAVEHLDGESDGDELGLPSATEVTEIPI
Sbjct: 961  VRHEGLESIAQTDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPI 1020

Query: 1021 MDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTLDKLFWTPFSEI 1080
            MDDLSPKQLSRQGSL NGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLT DKLFWTPFSEI
Sbjct: 1021 MDDLSPKQLSRQGSLLNGFNRRHSDDEDPQAGRVLSSGDIPSSTGNLTSDKLFWTPFSEI 1080

Query: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELS 1140
            QQMRMRDIQRSYFPELKSISNYTPKLLPTAH+FINKEGQKLHIFLSNEKFIVSDYEGELS
Sbjct: 1081 QQMRMRDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELS 1140

Query: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLL 1200
            SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPS+GSSDSDSSV+SEEHQFSSFDRLNLL
Sbjct: 1141 SIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSSGSSDSDSSVTSEEHQFSSFDRLNLL 1200

Query: 1201 DSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWN 1260
            DSLLPKTFKRAEHEG+IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCI+WN
Sbjct: 1201 DSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCINWN 1260

Query: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGI 1320
            AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQ+CLAKVLGI
Sbjct: 1261 AKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTCLAKVLGI 1320

Query: 1321 YQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380
            YQVTVR+PKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF
Sbjct: 1321 YQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNF 1380

Query: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLR 1440
            VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA+KKELVCGIIDYLR
Sbjct: 1381 VNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVCGIIDYLR 1440

Query: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500
            QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW
Sbjct: 1441 QYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQQNLHGPW 1500

Query: 1501 GACGIT-DRASSQTNAEENDLSS 1522
            GACG T D ASSQTNAEENDLSS
Sbjct: 1501 GACGGTIDHASSQTNAEENDLSS 1522

BLAST of Cp4.1LG06g09270 vs. ExPASy TrEMBL
Match: A0A5D3DIR8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G001430 PE=4 SV=1)

HSP 1 Score: 2434 bits (6308), Expect = 0.0
Identity = 1266/1605 (78.88%), Postives = 1367/1605 (85.17%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCHYCGAVITDS GK L NG+S  LDD+  +C+C+VCKENQE E M  DN++SSE   LS
Sbjct: 1    MCHYCGAVITDSNGKNLENGSSLKLDDADPMCLCRVCKENQEQEMMKSDNRNSSEAPTLS 60

Query: 61   PMPSLSSCDS-----------DCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEV 120
            PMPSLSSC+S           DCSTVANLD++DGQE  T SSQ+ IDCRQERMS N SEV
Sbjct: 61   PMPSLSSCNSCVSANYTSFKCDCSTVANLDVRDGQESATGSSQEDIDCRQERMSLNSSEV 120

Query: 121  VHSNGQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHV 180
              SN QLSGRDD SV++ +QE T N SGV VNA++EQS+SNW+D DLWDPPEPEDP+DH+
Sbjct: 121  ADSNDQLSGRDDGSVMSNNQETTQNASGVPVNADVEQSNSNWMDPDLWDPPEPEDPEDHM 180

Query: 181  EGGMGYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFI 240
            EGGMGY+DDDDD EF DS+EW+TSSSFSRSVDEASVSYR KE+KQRAMQQ+MNGK+K FI
Sbjct: 181  EGGMGYNDDDDD-EFGDSSEWSTSSSFSRSVDEASVSYRFKEEKQRAMQQVMNGKYKAFI 240

Query: 241  GHLLKFVGV-ASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTR 300
             HLLK V V ASSGEDSENWVDIVSSLSWEAATFLKPVVNGKA+DP AHVKVKCIATGTR
Sbjct: 241  RHLLKVVHVPASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPNAHVKVKCIATGTR 300

Query: 301  SQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHV 360
             QSQFVKGMVFKKHAAHKHMPTHCKNPKL+LI+GMLGEAPISRLSSFNSMDQENDFT HV
Sbjct: 301  KQSQFVKGMVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHV 360

Query: 361  IEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLM 420
            IEMIE CTANVILVEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPILTSETLM
Sbjct: 361  IEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLM 420

Query: 421  SQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKI 480
            SQ TRQCDAVYF+KIVEEHAG+LEGGK  TKTLMFIEGCPTRLGCTILLKGAHSDELK++
Sbjct: 421  SQKTRQCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRV 480

Query: 481  KVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA----- 540
            K+VVQ AVVMAF L+LETSF+VDQ+AMFATIPFGGVP+  SSDPQSPLEP SP       
Sbjct: 481  KLVVQCAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAES 540

Query: 541  ------------------QGASNGSNLEG----------EPYNPAIFSGLSSISDSLKRV 600
                              + ASN SN+E           EPYNPAIFSG SSISDSLKR 
Sbjct: 541  ASDQIEQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRA 600

Query: 601  MGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFDVEVRGSSDEENSIHE 660
            MGESF L SP  S SSYFGH  DLSGLVAKSDSIPST     QFD EVRGSSD ENS+HE
Sbjct: 601  MGESFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHE 660

Query: 661  QFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSRNALKGTMCEQSHFSH 720
            Q V HQST  EG GFHETAPN + D +QKK P DSQSILVLMSSRNALKGTMCEQSHFSH
Sbjct: 661  QSVSHQSTL-EGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSH 720

Query: 721  IVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKVL 780
            IVFYKNFD PLGKFLQENLLNQ N+CTVCGELPEAH+YYYAH RKQL IQVKQLP +KVL
Sbjct: 721  IVFYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVL 780

Query: 781  PGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSV 840
            PGETEGKLWMWSRCSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSD TLPSKSSV
Sbjct: 781  PGETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSV 840

Query: 841  CGHSLFGDFLYFFG--LGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITETENVY 900
            CGHSLFGDFLYFFG  LGNMVAMFRY+EVAIYTVSMPPQKLEFNSSMRQGHLI E ENVY
Sbjct: 841  CGHSLFGDFLYFFGYVLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVY 900

Query: 901  AKGMILFTEIAQFLKKIISERKSSTVN-------DFSLLEEMLNEERSEFEQNIQSSLTK 960
             KGM+LF+EIA  LKKIISER SS VN       DFS +EEMLNEERSEFE  IQ+SLTK
Sbjct: 901  TKGMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTK 960

Query: 961  KGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEPV----- 1020
            KGN D  FHK+L+LNRLLW+LLLESCIWD+RLQ L S+GVT NSGTSENVEPEPV     
Sbjct: 961  KGNLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMN 1020

Query: 1021 -----GHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVT 1080
                 G E  ESIA+ DD  +QQD+SVDEN L  KEIAVE  DGES GDEL LPSA EVT
Sbjct: 1021 SNINVGPEESESIAENDDSKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVT 1080

Query: 1081 EIPIMDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGD------IPSSTGNLTLD 1140
            EI I+DDLSPK+LSRQG+LSNGF+  HSD ED Q GRVLSSGD      IP S G+ TL 
Sbjct: 1081 EIQIIDDLSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLS 1140

Query: 1141 KLFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKF 1200
            KLF TPFSEI+QM +RDIQRSYFPELKSIS+YTPKLL  A DFI++EGQKLHI L+++ F
Sbjct: 1141 KLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNF 1200

Query: 1201 IVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEG------------LNKVPSNGSS 1260
            +VSDYEGELSSIVACVLALLKD   +TD HNEDSKGEG            LNKVPSNGSS
Sbjct: 1201 VVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSS 1260

Query: 1261 DSDSSVSSEEHQFSSFDRLNLLDSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDL 1320
            DS+SS+SSEE++FSSFDRLNLLDSL+ +TFKR++HEG+IKSLAKGKYLVNCPY NQFRDL
Sbjct: 1261 DSESSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDL 1320

Query: 1321 RRRCCPSELHYIASLSRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDY 1380
            R RCCPSELH+IASLSRCI+WNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMKFAP+Y
Sbjct: 1321 RSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEY 1380

Query: 1381 FKYINESFDMGNQSCLAKVLGIYQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKG 1440
            FKYINESFDMGNQ+CLAKVLGIYQVTVR+ KSGKE+RH+LMVMENLSFGRNIIRQYDLKG
Sbjct: 1381 FKYINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKG 1440

Query: 1441 ALHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDY 1500
            ALHAR+NPATN+FGEVLLDQNFVNDMNSSPLYV+N+AKRRLQRAIWNDT FLNS+NVMDY
Sbjct: 1441 ALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDY 1500

Query: 1501 SLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKF 1522
            SLLVGVDAEKKELVCGIIDYLRQYTWDKQLETW KSSL PKNVLPTVVSPKEYKRRFRKF
Sbjct: 1501 SLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKF 1560

BLAST of Cp4.1LG06g09270 vs. ExPASy TrEMBL
Match: A0A0A0K755 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_7G206930 PE=4 SV=1)

HSP 1 Score: 2269 bits (5879), Expect = 0.0
Identity = 1181/1490 (79.26%), Postives = 1282/1490 (86.04%), Query Frame = 0

Query: 102  MSQNWSEVVHSNGQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPE 161
            MS+N SEV HSN QLSGRDD SV++ SQE T N SGV VNA++EQS+SNW+D DLWDPPE
Sbjct: 1    MSRNSSEVAHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPE 60

Query: 162  PEDPQDHVEGGMGYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIM 221
            PEDP+DH+EGGMGY+DDDD+ EF D++EW+T+SSFSRSVDE SVSYR KE+KQRA+QQ+M
Sbjct: 61   PEDPEDHMEGGMGYNDDDDE-EFGDNSEWSTASSFSRSVDETSVSYRFKEEKQRALQQVM 120

Query: 222  NGKFKVFIGHLLKFVGVASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVK 281
            NGK K FI  LLK       GEDSENWVDIVSSLSWEAATFLKPVVNGKA+DP+A+VKVK
Sbjct: 121  NGKHKAFIRDLLKL------GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVK 180

Query: 282  CIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQE 341
            CIATGTR+QSQFVKGMVFKKHAAHKHMPTHCKNPKLILI+GMLGEAPISRLSSFNSMDQE
Sbjct: 181  CIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQE 240

Query: 342  NDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPI 401
            NDFT HVIEMIE CTANVILVEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPI
Sbjct: 241  NDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPI 300

Query: 402  LTSETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAH 461
            LTSETLMSQ TRQCDAVYF+KIVEEHAG+LEGGK PTKTLMFIEGCPTRLGCTILLKGAH
Sbjct: 301  LTSETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCPTRLGCTILLKGAH 360

Query: 462  SDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSP 521
            SDELK++K+VVQ AVVMAFHL+LETSF+VDQRAMFATIPFGGVPAT SSD QSPLEP SP
Sbjct: 361  SDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDRQSPLEPRSP 420

Query: 522  N---AQGASN--------------------GSNLE----------GEPYNPAIFSGLSSI 581
            N   A+ AS+                    GSN+E           EPYNPAIFSG SSI
Sbjct: 421  NVGKAESASDKSEQPTNICISRGSHEETSTGSNMELAEKLIMSSEPEPYNPAIFSGFSSI 480

Query: 582  SDSLKRVMGESFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFDVEVRGSSD 641
            SDSLKRVMGESF  +SP QS SSYFGHG DLSGLVAKS SIPSTP    QFDVEV+GSSD
Sbjct: 481  SDSLKRVMGESFLSSSPYQSLSSYFGHGSDLSGLVAKSGSIPSTPQAVYQFDVEVKGSSD 540

Query: 642  EENSIHEQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSRNALKGTMC 701
            EENS+HE  V HQST  EG GFHETA NYS D +QKK   DSQSILVLMSSRNALKG +C
Sbjct: 541  EENSVHESSVSHQSTL-EGLGFHETALNYSVDTMQKKMSLDSQSILVLMSSRNALKGAIC 600

Query: 702  EQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQ 761
            EQSHFSHIVFYK+FD PLGKFLQENLLNQ N+CTVCGELPEAH Y++AH RKQL IQVKQ
Sbjct: 601  EQSHFSHIVFYKHFDVPLGKFLQENLLNQNNLCTVCGELPEAHIYFFAHYRKQLSIQVKQ 660

Query: 762  LPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCT 821
            LP +KVLPGETEGKLWMWSRCSKCKS GGPSKSTKRV ISTAARGLSFGKFLELCFSD T
Sbjct: 661  LPGDKVLPGETEGKLWMWSRCSKCKSTGGPSKSTKRVPISTAARGLSFGKFLELCFSDDT 720

Query: 822  LPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITE 881
            L SKS VCGHSLFGDFLYFFGLGNMVAM RY+EVAIYTVSMPPQKLEFNSSMRQGH I E
Sbjct: 721  LLSKSPVCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHFIRE 780

Query: 882  TENVYAKGMILFTEIAQFLKKIISERKSSTVN-------DFSLLEEMLNEERSEFEQNIQ 941
            TENVY KG++LFTEIA  LKKIISERKSS VN       DFS +EEMLNEERSEFE NIQ
Sbjct: 781  TENVYTKGVLLFTEIAHSLKKIISERKSSNVNHPQGFPNDFSFVEEMLNEERSEFEINIQ 840

Query: 942  SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP- 1001
            +SLTKKGNPDF     L+LNRLLW+LLLESCIWD+RLQ L S+GVT NSG+SE VEPEP 
Sbjct: 841  NSLTKKGNPDF-----LNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGSSEIVEPEPL 900

Query: 1002 ---------VGHEGLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPS 1061
                     VG E LESIA+ D+  VQQD+S+DEN L  KEI VE  DGES GDEL LPS
Sbjct: 901  LLKMNSNIDVGPEELESIAENDETKVQQDISLDENVLPLKEITVEGSDGESGGDELNLPS 960

Query: 1062 ATEVTEIPIMDDLSPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGD------IPSSTG 1121
            A EVTEIPI+DD SPK+LSRQG+LSNGF+  HSD ED Q GRVLSSGD      IP S G
Sbjct: 961  AIEVTEIPIIDDPSPKKLSRQGTLSNGFDYHHSDYEDSQVGRVLSSGDLHIDRTIPISIG 1020

Query: 1122 NLTLDKLFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFL 1181
            + TL KLF TPFSEI+QM +RDIQRSYFPELKSIS+YTPKLLP A DFI++EGQKLHI L
Sbjct: 1021 DSTLSKLFCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHL 1080

Query: 1182 SNEKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEG------------LNKVP 1241
            +N+ F+VSDYEGELSSIVACVLALLKD   +TD HNEDSKGEG            LNKVP
Sbjct: 1081 ANDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVP 1140

Query: 1242 SNGSSDSDSSVSSEEHQFSSFDRLNLLDSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFN 1301
            SNGSSDSDSS+SSEE++FSSFDRLNLLDSL+ +TFKR+++EG+IKSLAKGKYLVNCPY N
Sbjct: 1141 SNGSSDSDSSISSEEYRFSSFDRLNLLDSLVSETFKRSDYEGVIKSLAKGKYLVNCPYIN 1200

Query: 1302 QFRDLRRRCCPSELHYIASLSRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMK 1361
            QFRDLR RCCPSELH+IASLSRCI+WNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMK
Sbjct: 1201 QFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMK 1260

Query: 1362 FAPDYFKYINESFDMGNQSCLAKVLGIYQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQ 1421
            FAP+YFKYINESFDMGNQ+CLAKV+GIYQVTVR+PKSGKE+RH+LMVMENLSFGRNIIRQ
Sbjct: 1261 FAPEYFKYINESFDMGNQTCLAKVVGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQ 1320

Query: 1422 YDLKGALHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSM 1481
            YDLKGALHAR+NPATN+FGEVLLDQNFVNDMNSSPLYV+N+A RRLQRAIWNDTAFLNS+
Sbjct: 1321 YDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAMRRLQRAIWNDTAFLNSI 1380

Query: 1482 NVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKR 1522
            NVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETW KSSL PKNVLPTV+SPKEYKR
Sbjct: 1381 NVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKR 1440

BLAST of Cp4.1LG06g09270 vs. ExPASy TrEMBL
Match: A0A6J1F0I5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438379 PE=4 SV=1)

HSP 1 Score: 2246 bits (5820), Expect = 0.0
Identity = 1177/1570 (74.97%), Postives = 1295/1570 (82.48%), Query Frame = 0

Query: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60
            MCH+CGAV+ DS  ++L NG+S  LDD+G +C CKVC ENQE ETM  D+ SSSE LMLS
Sbjct: 1    MCHHCGAVVADSAAQKLENGSSVKLDDAGAMCFCKVCNENQEQETMKRDDGSSSESLMLS 60

Query: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120
            PMPS S+C+SDCST+ +LDI++GQEG+T SSQ+ ID +QER SQN S VVHSN QL GRD
Sbjct: 61   PMPSPSTCNSDCSTMGSLDIRNGQEGSTGSSQEDIDHKQERRSQNLSGVVHSNVQLGGRD 120

Query: 121  DESVITIS-QEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGY--DD 180
            DESV+ I+ QEATHNG GV VNAE E+S+S+W+DT+LWDPPEPEDP DH+EGGMGY  DD
Sbjct: 121  DESVMPINNQEATHNGFGVPVNAEFEKSNSSWMDTNLWDPPEPEDPDDHMEGGMGYYDDD 180

Query: 181  DDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVG 240
            DDDDDEF DSTEW+ SSSFSRSVDEASVS+R KE+KQRAMQ++MNGK+K FI HLL  VG
Sbjct: 181  DDDDDEFGDSTEWSMSSSFSRSVDEASVSHRSKEEKQRAMQEVMNGKYKAFIHHLLNLVG 240

Query: 241  VASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGM 300
            V SSGED ENW DIV SLSWEAATFLKP+VNGKA+DP AHVKVKCIA+GTRSQSQFVKGM
Sbjct: 241  VPSSGEDGENWADIVCSLSWEAATFLKPLVNGKAMDPGAHVKVKCIASGTRSQSQFVKGM 300

Query: 301  VFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTA 360
            VFKKHAAHKHM T+ KNP+LILI+G LGEAPIS LSSFNSMDQENDFT +VIEMIE CTA
Sbjct: 301  VFKKHAAHKHMVTNWKNPRLILIQGRLGEAPISGLSSFNSMDQENDFTNYVIEMIETCTA 360

Query: 361  NVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDA 420
            NVI+VEKT SRVIQEA+LK+ +TLVLDMKLHRLERIALCTGSPILTSETL SQ  RQCDA
Sbjct: 361  NVIIVEKTASRVIQEAILKRCITLVLDMKLHRLERIALCTGSPILTSETLASQKARQCDA 420

Query: 421  VYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVV 480
            VYF+K+VE H+G LEGGKR TKTLMFIEGCP RLGCTILLKGA S ELK++K+VVQ AVV
Sbjct: 421  VYFQKVVE-HSGGLEGGKRSTKTLMFIEGCPARLGCTILLKGARS-ELKRVKLVVQRAVV 480

Query: 481  MAFHLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA-------------- 540
            MAFHL+LETSFLVDQRAMFATIPFGGV   VS DPQSPLEPCS N               
Sbjct: 481  MAFHLILETSFLVDQRAMFATIPFGGVSTMVSPDPQSPLEPCSSNILQVEKANDKSEPCV 540

Query: 541  -------------QGASNGSNL----------EGEPYNPAIFSGLSSISDSLKRVMGESF 600
                         + +SNGSNL          E EPYNPAI SG SSISDS K+VMGESF
Sbjct: 541  SLLTDIYISSGPHEESSNGSNLKPVEKPIIPSELEPYNPAILSGFSSISDSSKKVMGESF 600

Query: 601  FLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFDVEVRGSSDEENSIHEQFVPH 660
             L+S   S SSYFGH +D  GL  KSDSIPSTP  A++FDV+VRGSSDEENS HEQ   H
Sbjct: 601  LLSSTHHSLSSYFGHERDWIGLAPKSDSIPSTPQAANRFDVKVRGSSDEENSGHEQSASH 660

Query: 661  QSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSRNALKGTMCEQSHFSHIVFYK 720
            QS   EG GFHE APN SE+ +QKK+  DSQSILVLMSSRNALKGTMCEQSHF HIVFY+
Sbjct: 661  QSAL-EGLGFHENAPNCSEETMQKKSSLDSQSILVLMSSRNALKGTMCEQSHFLHIVFYR 720

Query: 721  NFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKVLPGETE 780
            NFD PLGKFLQENLLNQ N CTVC ELPEAH+YY+AH  KQL IQV+QLP  K+LPG+TE
Sbjct: 721  NFDVPLGKFLQENLLNQTNFCTVCSELPEAHFYYFAHYGKQLSIQVQQLPGYKLLPGQTE 780

Query: 781  GKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSL 840
            GKLWMWSRCSKCKSKG PS STK VLISTAAR LSFGKFLELCFSD TLPSKSS CGHSL
Sbjct: 781  GKLWMWSRCSKCKSKGEPSTSTKMVLISTAARSLSFGKFLELCFSDETLPSKSSGCGHSL 840

Query: 841  FGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYAKGMILF 900
            FGDFLYFFGLGN VAMFRY+  AIYTVSMPPQKL+FNSSMRQGHLI ETENV  KG +LF
Sbjct: 841  FGDFLYFFGLGNRVAMFRYSAAAIYTVSMPPQKLDFNSSMRQGHLIRETENVKKKGKLLF 900

Query: 901  TEIAQFLKKIISERKSSTV------NDFSLLEEMLNEERSEFEQNIQSSLTKKGNPDFPF 960
            TEIA  LKKI SE +SS V      NDFS +EEMLN+ERSEFE  IQ+SL         F
Sbjct: 901  TEIAHSLKKISSECRSSNVKPQCFLNDFSSIEEMLNQERSEFEVKIQNSLA--------F 960

Query: 961  HKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEPVG----------HE 1020
            HKYLSLN+LLWELLLESCIWD+RLQ LTS+G+T   GTSENVEPE V           HE
Sbjct: 961  HKYLSLNQLLWELLLESCIWDRRLQSLTSLGLTSTPGTSENVEPESVTMNMNSNIDARHE 1020

Query: 1021 GLESIAQTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDL 1080
              ESIA  DD  VQQD+S DEN L  KEIAVE LDGES  DE   PS TEVTEI IMDDL
Sbjct: 1021 ESESIAANDDTEVQQDISWDENVLPMKEIAVEGLDGESGCDENHSPSVTEVTEIRIMDDL 1080

Query: 1081 SPKQLSRQGSLSNGFNRRHSDDEDPQAGRVLSSGD------IPSSTGNLTLDKLFWTPFS 1140
            SPK LSRQGSLSNGFN+ H DDED Q  RV SSGD      IP  T + +L KL W P  
Sbjct: 1081 SPKHLSRQGSLSNGFNQHHLDDEDSQV-RVASSGDMHVDRTIPVLTADSSLGKLLWAPCV 1140

Query: 1141 EIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGE 1200
            EI+QM +RDIQRS+FPELKS S+YTPKL+P A  F N+EGQKLHI L+ + FIVSDYEGE
Sbjct: 1141 EIRQMHIRDIQRSFFPELKSFSSYTPKLIPAASAFTNEEGQKLHIRLAKDNFIVSDYEGE 1200

Query: 1201 LSSIVACVLALLKDLSLETDLHNEDSKGEG------------LNKVPSNGSSDSDSSVSS 1260
            LSSIVACVLALL+DLSL+TD HNEDSKGEG            LNKVPSNGSSDSDSS +S
Sbjct: 1201 LSSIVACVLALLEDLSLQTDSHNEDSKGEGGGVSQPNLSSQSLNKVPSNGSSDSDSSAAS 1260

Query: 1261 EEHQFSSFDRLNLLDSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSE 1320
            + +QFS+F+RLNLLDSL+P+TFKR +HEG+IKSLA GKY+VNCPYFNQFRDLR +CCPSE
Sbjct: 1261 DAYQFSNFERLNLLDSLVPETFKRTDHEGVIKSLANGKYVVNCPYFNQFRDLRSQCCPSE 1320

Query: 1321 LHYIASLSRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESF 1380
            LHYIASLSRCI WNAKGGKS SFFAKTLDDRFIIKEIKRTEY+SFMKFAP+YFKYI +SF
Sbjct: 1321 LHYIASLSRCIKWNAKGGKSNSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYIKQSF 1380

Query: 1381 DMGNQSCLAKVLGIYQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNP 1440
            DMGNQ+CLAKVLGIYQV VR+PKSGKE+RH++MVMENLSFGRNIIRQYDLKGALHARYNP
Sbjct: 1381 DMGNQTCLAKVLGIYQVAVREPKSGKEMRHDVMVMENLSFGRNIIRQYDLKGALHARYNP 1440

Query: 1441 ATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDA 1496
            A N+FGEVLLDQNFV DMNSSP+YV+N+AKRR+QRAIWNDTAFLNS+NVMDYSLLVGVDA
Sbjct: 1441 AANNFGEVLLDQNFVKDMNSSPIYVSNRAKRRMQRAIWNDTAFLNSINVMDYSLLVGVDA 1500

BLAST of Cp4.1LG06g09270 vs. TAIR 10
Match: AT1G34260.1 (FORMS APLOID AND BINUCLEATE CELLS 1A )

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 676/1517 (44.56%), Postives = 914/1517 (60.25%), Query Frame = 0

Query: 59   LSPMPSLSSCDSDCSTVANLDIKDGQEG-----TTRSSQDSIDCRQERMSQNWSEVVHSN 118
            ++P  SLSS +   S   ++D      G     ++ SSQ+ ++  +E         V   
Sbjct: 1    MTPSNSLSSSERSLSGECSVDGNSCDRGIEDECSSHSSQEDVELTKE-------VKVDRL 60

Query: 119  GQLSGRDDESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGM 178
             + S      ++ I  E +   S   V   +     +  D  +W+PPEPE+P+D V+G  
Sbjct: 61   ERKSKSMPSDILDILDEKSKENSVENVQF-LSDREDDSDDVPVWEPPEPENPEDEVDGVF 120

Query: 179  GYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLL 238
                 DDDD+  D ++WN +S      DE+S   ++ E+ +R M +  + KFK  +  L+
Sbjct: 121  A----DDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQLI 180

Query: 239  KFVGVASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQF 298
            K  G   S E+S  W +IV+ L WEAA+ LKP ++GK+VDP  ++KVKCIATG+   S+ 
Sbjct: 181  KSAGF--SIEESGYWFEIVARLCWEAASMLKPAIDGKSVDPTEYIKVKCIATGSCVDSEV 240

Query: 299  VKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQEND----FTKHVI 358
             KG+VFKKHAA KHM T  ++P+++L+EG+LG  PIS  SS  S++Q+N+    + K V+
Sbjct: 241  FKGLVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVV 300

Query: 359  EMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMS 418
            ++IE    +V+LVEK+ SR IQ+ +L KG+TLV DMKLHRL+RI+ C GSPIL+ ++L S
Sbjct: 301  DIIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSS 360

Query: 419  QNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIK 478
            Q  + CD+   EKIVEEH    E  K+PTKTLMF+EGCPTRLGCTILLKG HS+ LKK+K
Sbjct: 361  QKLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVK 420

Query: 479  VVVQIAVVMAFHLMLETSFLVDQRAMFATI------------------------PFGGVP 538
             VVQ + ++A+HLMLE SFL D+  MF+TI                        P   V 
Sbjct: 421  EVVQYSFILAYHLMLEASFLADRHTMFSTIFAKEATSCVVEIENFSPSPSPRESPSEAVD 480

Query: 539  ATVSSDPQSPLEPCSPNAQG------ASNGSNL-EGEPYNPAIFSGLSSISDSLKRVMGE 598
              VS+         +  A G       S+G ++   EPYNP IF+G SS+S  L      
Sbjct: 481  IPVSNGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARL------ 540

Query: 599  SFFLASPCQSFSSYFGHGKDLSGLVAKSDSIPSTPPGADQF----DVEVRGSSDEENSIH 658
                       S Y G  ++   +    D+  ST    D      +     + D++  + 
Sbjct: 541  -----------SKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLL 600

Query: 659  EQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPFDSQSILVLMSSRNALKGTMCEQSHFS 718
            +  +P  S+ D+G        N S+ +   ++  +SQSILVL+S RNAL+G MC+Q HFS
Sbjct: 601  DPELPVNSSSDDG-------DNKSQTENDIESTLESQSILVLVSKRNALRGIMCDQRHFS 660

Query: 719  HIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKV 778
            HI FYK+FD PL KFL++ + NQ+N+C  C E PEAH YYYAH  KQL IQ+K++PV K 
Sbjct: 661  HIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKG 720

Query: 779  LPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSS 838
            L GE +GK+WMWSRC KCK+K    KSTKRVLISTAAR LSFGKFLEL FS  T  ++SS
Sbjct: 721  LAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSS 780

Query: 839  VCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYA 898
             CGHS   DFL+FFGLG+MVAM  Y++VA YTVS+PP KLE +  ++ G L  E + V+ 
Sbjct: 781  SCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFT 840

Query: 899  KGMILFTEIAQFLKKIISERKSSTVND------FSLLEEMLNEERSEFEQNIQSSLTKKG 958
            KG+ LF + A FLK++ S+  +S +         S +EE+L  ER  FE+NI++S  K  
Sbjct: 841  KGISLFEDAAGFLKRLRSQFTNSDLRYQRARKLLSNIEELLKHERCIFEENIKNSFDKAK 900

Query: 959  NPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEPVGHEGLES 1018
              D   H+ L LNR+ WELLL++ IW+ RLQ L      L+     + +   +  +GL++
Sbjct: 901  TIDDVSHRLLRLNRMRWELLLQALIWNYRLQSL-----VLSDRLLPSSDETKIYEQGLKT 960

Query: 1019 IAQTDDRNVQQDVSVDENALHP--KEIAVEHLD------GESDGDELGLPSATEVTEIPI 1078
            +++      + D  V ++  +       VEH D         D D++      E  E   
Sbjct: 961  VSEAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGASVGDNDQMAESYVPEDNESQT 1020

Query: 1079 MDDLSPKQLSRQGSLSNGF-----------NRRHSDDEDPQAGRVLSSGDIPSSTGN--L 1138
            +   SP   S    ++N F           N + +D   P  G  L   ++ +S G   L
Sbjct: 1021 LCSSSPDTTS---PINNHFDTHLAVNVHSTNGQEADKSIPVTGESLDD-EVSTSNGPHIL 1080

Query: 1139 TLDKLFWTPFSEIQQMRMRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSN 1198
              D+ FW PF E++  R+ DI++ Y  + + ++N+T + L T +  I +E  +L I L +
Sbjct: 1081 GWDEWFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQIITEESSRLRISLRD 1140

Query: 1199 EKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKVPSN--GSSDSDSSVS 1258
            + FIVSDYE ELSS++AC LA          L+NE+SK      +  +  G  D++    
Sbjct: 1141 DDFIVSDYEDELSSLIACALA---------HLNNEESKKPLSRCIHGSLQGFLDNNQDSK 1200

Query: 1259 SEEHQFSSF--DRLNLLDSLLPK----TFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLR 1318
              +   S F  +  N L++L P     TF      G +KS+ K KY +   Y + FRDLR
Sbjct: 1201 QTDRDVSRFSSESTNRLETLPPPEVLVTF------GSVKSVGKPKYSIVSLYADDFRDLR 1260

Query: 1319 RRCCPSELHYIASLSRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYF 1378
            +RCC SEL YIASLSRC  W+AKGGKSKS FAKTLDDRFI+KEIK+TEYESF+ FA +YF
Sbjct: 1261 KRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYF 1320

Query: 1379 KYINESFDMGNQSCLAKVLGIYQVTVRDPK-SGKEVRHELMVMENLSFGRNIIRQYDLKG 1438
            KY+ +S+D+GNQ+CLAKVLGI+QVTVR PK  GKE+RH+LMVMENLSF R + RQYDLKG
Sbjct: 1321 KYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKG 1380

Query: 1439 ALHARYN-PATNDFGEVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMD 1494
            ALHAR+   + N   +VLLDQNFVNDMN SPLYV+  +K+ LQRA++NDT+FL S+NVMD
Sbjct: 1381 ALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINVMD 1440

BLAST of Cp4.1LG06g09270 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 758.1 bits (1956), Expect = 1.4e-218
Identity = 539/1477 (36.49%), Postives = 771/1477 (52.20%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDDEFDDSTEW---NTSSSFSRSVDEASVSYRIKEQ 215
            LW PPEPE+ +D  E  +    D++D+E D S EW     S+SF     E     R  E+
Sbjct: 318  LWVPPEPENEEDERESAL---FDEEDNEGDASGEWGYLRPSTSFGSG--EYRGEDRTTEE 377

Query: 216  KQRAMQQIMNGKFKVFIGHLLKFVGVASSGED-SENWVDIVSSLSWEAATFLKP-VVNGK 275
             ++AM+ +++G F+  +  LL+   ++ S E+  E+W++I++SLSWEAA  LKP +    
Sbjct: 378  HKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLLKPDMSKSG 437

Query: 276  AVDPEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS 335
             +DP  +VKVKC+A+G R  S  VKG+V KK+  ++ M T  +  +L+++ G L    +S
Sbjct: 438  GMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGGGLEYQRVS 497

Query: 336  -RLSSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLH 395
             +LSSF++ + QE D  K  +  I     N++LVEK+ SR  QE +L K ++LVL++K  
Sbjct: 498  NQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDISLVLNIKRP 557

Query: 396  RLERIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGC 455
             L+RIA CTG+ I+ S + L SQ    C+    ++  EEH    + GK+  KTLM+ E C
Sbjct: 558  LLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGKKVVKTLMYFEHC 617

Query: 456  PTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRA---------MFAT 515
            P  LG TILL+GA+ DELKK+K VVQ  V  A+HL LETSFL D+ A         +   
Sbjct: 618  PKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGASPELPLNSPITVA 677

Query: 516  IP---------FGGVPA-TVSSDPQSP--LEPCSPNAQGASNGSNLEGEPYNPAIFSGLS 575
            +P            VP  TVS+  +SP  L    P    +   S L     N +I   + 
Sbjct: 678  LPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELLSTTTNLSIQKDIP 737

Query: 576  SI---SDSLKRVMGESFFLASPCQSFS----SYFGHGKDLSGLVAKSDSIPSTPPGADQF 635
             I   S    R +  SF  +    S +           DLSG      S+P   P AD+ 
Sbjct: 738  PIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSG-----RSVPVDTP-ADKS 797

Query: 636  DVEVRGSSDEENSIH---EQFVPHQSTF-------DEGFGFHETAPNYSEDKLQKKT--- 695
            +  V       NS+H   + FV   S         ++  G   T      ++  K+T   
Sbjct: 798  NPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNEKPKETQSQ 857

Query: 696  -------PFDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKN 755
                   P D QSILV +SSR+  KGT+CE+SH   I +Y +FD PLG+FL+++L +Q  
Sbjct: 858  KEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRDHLFDQSY 917

Query: 756  VCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPS 815
             C  C    EAH + Y H +  L I VK+L  + +LPGE EGK+WMW RC +C    G  
Sbjct: 918  RCRSCEMPSEAHVHCYTHRQGSLTISVKKLQ-DYLLPGEKEGKIWMWHRCLRCPRLNGFP 977

Query: 816  KSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRY 875
             +T RV++S AA GLSFGKFLEL FS+    S+ + CGHSL  D L F+G GNMVA FRY
Sbjct: 978  PATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFRY 1037

Query: 876  TEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTV 935
              + +++V +PP  L FN    Q  +  ET+ V  +  +LF+E+   + +I  +     +
Sbjct: 1038 ATIDVHSVYLPPSILSFNYE-NQDWIQRETDEVIERAELLFSEVLNAISQIAEKGFRRRI 1097

Query: 936  NDFSLLEEMLNEERSEFEQNIQSSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQ 995
             +   LEE+L +E++EFE+N+Q  L ++ N   P    L L R+  +LL +S +WD RL 
Sbjct: 1098 GE---LEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLI 1157

Query: 996  LLTSVGVTLNSGTSENVEPE----------PVGHEGLESIAQTDDRNVQQ------DVSV 1055
              +++    NS  ++  E E          P  + G  S+    + N+        D   
Sbjct: 1158 NASTLHKLENSDDTKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGS 1217

Query: 1056 DENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDLSPKQLSRQGSL-------- 1115
              N     +   +    + DG E+        T  P+ + +  ++    G +        
Sbjct: 1218 LNNVQKEADTNSDLYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSAT 1277

Query: 1116 --SNGFNRRHSDDEDPQAGRV---------------LSSG-------------------- 1175
              +     R +  E P   +V               +S G                    
Sbjct: 1278 LDAAWIGERQTSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVS 1337

Query: 1176 -DIPSSTGNLTLDKLFW--TPFSEIQQMRMRDIQRSYF---PELKSISNYTPKLLPTAHD 1235
              +PS     + D + W   PF        R I +++     +L +   ++P  + +  +
Sbjct: 1338 PALPSKNYENSEDSVSWLSVPFLNF----YRSINKNFLLSSQKLDTFGEHSPIYISSFRE 1397

Query: 1236 FINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALLKDLSLETDLHNEDSKGEGLNKV 1295
               + G +L + +     +V  Y+ E +S++A  L     +S E       ++GE L   
Sbjct: 1398 AELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYAL-----MSPEYQ-RQTSAEGESLVSY 1457

Query: 1296 PSN------------GSSDSDSSVSSEEHQFSSFDRLNLLDSLLPKTFKRAEHE----GI 1355
            PS               S S+ SV       SS    +LLD   P ++ +A H     G 
Sbjct: 1458 PSELNIPRPVDDTIFDPSRSNGSVDESILSISSSRSTSLLD---PLSYTKALHARVSYGE 1517

Query: 1356 IKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWNAKGGKSKSFFAKTLDD 1415
              +L K KY V C Y  +F  LR  C PSEL YI SLSRC  W A+GGKS  FFAKTLDD
Sbjct: 1518 DGTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDD 1577

Query: 1416 RFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGIYQVTVRDPKSGKEVRH 1475
            RFIIK++ +TE ESF+KFAP YFKY++ES    + +CLAK+LGIYQV  +  KSGKE + 
Sbjct: 1578 RFIIKQVTKTELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKM 1637

Query: 1476 ELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFVNDMNSSPLYVNNQAK 1491
            ++++MENL FGR + R YDLKG+  ARYNP ++   +VLLDQN +  M +SP++V N+AK
Sbjct: 1638 DVLIMENLLFGRTVKRLYDLKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAK 1697

BLAST of Cp4.1LG06g09270 vs. TAIR 10
Match: AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C )

HSP 1 Score: 738.8 bits (1906), Expect = 8.8e-213
Identity = 505/1430 (35.31%), Postives = 774/1430 (54.13%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQR 215
            +W PP PED  D  E    + DD+DDD  D +TE++ SSSFS  +       ++ E    
Sbjct: 263  IWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHI---PTKEKLGENSNE 322

Query: 216  AMQQIMNGKFKVFIGHLLKFVGVASSGEDSE-NWVDIVSSLSWEAATFLKPVVN-GKAVD 275
             ++ +++  F+  +  LL+   ++ S + S   W+DIV++L+W+AA F+KP    G ++D
Sbjct: 323  PLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTALAWQAANFVKPDTRAGGSMD 382

Query: 276  PEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RL 335
            P  +VK+KC+A+G +++S  ++G+V  K+  HK M +  KNP+++L+ G L    ++ +L
Sbjct: 383  PGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQL 442

Query: 336  SSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLE 395
            +SFN+ + QEN+  K +I  IE    NV+LVEK+ S   Q+ +L+K ++LVL++K   L+
Sbjct: 443  ASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLLD 502

Query: 396  RIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTR 455
            RIA CTG+ +  S +++ +     C+    E+++E+H    +  ++P++TLM+ EGCP R
Sbjct: 503  RIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGNQSNRKPSRTLMYFEGCPRR 562

Query: 456  LGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPF--GGVPATV 515
            LGCT++L+G+  +ELKK+K V+Q AV  A+HL LETSFL D+ A    I     G+  T 
Sbjct: 563  LGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRLKQPGMVRTA 622

Query: 516  S------------------SDPQSPLEPCSPNAQGASNGSNLE-----GEPYNPA-IFSG 575
            S                  +D Q+ LE  +   +  +     E      E ++P  IF  
Sbjct: 623  SQRRIIDEGISLITQSPTETDSQALLETAAHEDEHTAPMPEHEVCESLCEDFDPTQIFPP 682

Query: 576  LSSISDSLKRVMGESFFLASPCQSFSSYFGHGKDL-SGLVAKSDSIPSTPPGADQFDVEV 635
             S +       +   F      +S+SS      DL    +  S  IP TP      + E 
Sbjct: 683  SSEVETEQSDTLNGDFANNLVTRSYSS--NQLNDLHEPTLCLSSEIPETPTQQPSGE-ED 742

Query: 636  RGSSDEENS-IHEQFVPHQSTFDEGFGFHETAPNYSEDKLQK--KTPFDSQSILVLMSSR 695
             G  +EEN  ++ Q +P   +F            Y +D   +        QSILV  SSR
Sbjct: 743  NGRGEEENQLVNPQDLPQHESF------------YEDDVSSEYFSAADSHQSILVSFSSR 802

Query: 696  NALKGTMCEQSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRK 755
              LK ++CE+S    I FY +FD PLG++L+++L ++ + C  C EL +AH   Y+H   
Sbjct: 803  CVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVLCYSHQNG 862

Query: 756  QLCIQVKQLPVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFL 815
             L I V++LP  K LPGE +GK+WMW RC +C    G   +T+RV++S AA GLSFGKFL
Sbjct: 863  NLTINVRRLPSMK-LPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWGLSFGKFL 922

Query: 816  ELCFSDCTLPSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSM 875
            EL FS+    ++ + CGHSL  D L F+G GNMVA FRY+ + I TV +PP  LEFNS  
Sbjct: 923  ELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSMLEFNSHP 982

Query: 876  RQGHLITETENVYAKGMILFTEIAQFLKKIISERKSSTVND------------FSLLEEM 935
            +Q  + TE   +  K   ++TEI+  L ++  E KSS +                L++++
Sbjct: 983  QQEWIRTEAAELVGKMRTMYTEISDMLNRM--EEKSSLLEPEQSEACDLHSRIIGLIDQL 1042

Query: 936  LNEERSEFEQNIQ----SSLTKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLL--- 995
            + +E+ E++  +Q     +L  +G+ D      L LNRL   L++ +  WD +L LL   
Sbjct: 1043 V-KEKDEYDDALQPIFEENLQIQGSLDI-----LELNRLRRALMIGAHAWDHQLYLLNSQ 1102

Query: 996  ---TSVGVTLNSGTSENVE---PEPVGHEGLESIAQTD-----DRNVQQDVSVDENALHP 1055
                SV  T +     N E   P  +     E   + D     D     D    EN   P
Sbjct: 1103 LKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPSP 1162

Query: 1056 KEIAVEHLDGESDGDELGLPSATEVTEIPIMD--DLSPKQLSRQGSLSNGFNRRHSDDED 1115
                 E +D    G    L  A  + E       + S ++L+R         R  S D  
Sbjct: 1163 GTSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARP-------IRVQSFDSA 1222

Query: 1116 PQAGRVLSSGDIPSSTGNLTLDKLFWT-PFSEIQQMRMRDIQRSYFPELKSISNYTPKLL 1175
             +    +  G  PSS    TL     +  +  + +  + ++ R+Y   L         ++
Sbjct: 1223 IRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDLIV 1282

Query: 1176 PTAHDFINK-----EGQKLHIFLSN-EKFIVSDYEGELSSIVACVLALLKDLSLETDLHN 1235
             +A  +I+      +G ++ I        +V  Y+ + +S+V+              +++
Sbjct: 1283 GSAPTYISSASQMADGARMLIPQRGLNDIVVPVYDDDPASVVSYA------------INS 1342

Query: 1236 EDSKGEGLNK-VPSNGSSDSDSSVSSEEHQFSSFDRLNL-LDSL---------------- 1295
            ++ K   +NK + S+ SS + ++  SE   FS++  L++ +D +                
Sbjct: 1343 KEYKEWIVNKGLASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKSPH 1402

Query: 1296 LPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIHWNAKG 1355
            L  +F          +  K K+ V C +  QF  LR+ CCPSE+ ++ SLSRC  W+A+G
Sbjct: 1403 LTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQG 1462

Query: 1356 GKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVLGIYQV 1415
            GKS  +FAK+LD+RFIIK++ +TE +SF  FAP+YFKY+ ES   G+ +CLAK+LGIYQV
Sbjct: 1463 GKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQV 1522

Query: 1416 TVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQNFVND 1475
            +++ PK GKE + +LMVMENL + R I R YDLKG+  +RYNP T+   +VLLD N +  
Sbjct: 1523 SIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLET 1582

Query: 1476 MNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDYLRQYT 1491
            + + P+++ ++AKR L+RAIWNDT FL S++VMDYSLLVG D E+KELV GIID++RQYT
Sbjct: 1583 LRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQYT 1642

BLAST of Cp4.1LG06g09270 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 737.6 bits (1903), Expect = 2.0e-212
Identity = 520/1435 (36.24%), Postives = 748/1435 (52.13%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQR 215
            LW PPEPE+ +D  E  +  DDD D+ +  D      S+SF+         +  K++   
Sbjct: 311  LWLPPEPENEEDEREAVLS-DDDGDEGDRGDWGYLRPSNSFNEK------DFHSKDKSSG 370

Query: 216  AMQQIMNGKFKVFIGHLLKFVGVASSGE-DSENWVDIVSSLSWEAATFLKP-VVNGKAVD 275
            AM+ ++ G F+  +  LL+   +    E D E W+DI++SLSWEAAT LKP       +D
Sbjct: 371  AMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMD 430

Query: 276  PEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RL 335
            P  +VKVKCI  G RS+S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +L
Sbjct: 431  PGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQL 490

Query: 336  SSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLE 395
            SSF++ + QE D  K  +  I+    +++LVEK+ SR  QE +L K ++LVL++K   LE
Sbjct: 491  SSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLE 550

Query: 396  RIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTR 455
            RI+ CTG+ I+ S + L S     CD  + EK VE H    +  K+  KTLMF +GCP  
Sbjct: 551  RISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKP 610

Query: 456  LGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSS 515
            LGCTILLKGAH DELKK+K V+Q  V  A+HL LETSFL D+ A    +P    P TV+ 
Sbjct: 611  LGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVAL 670

Query: 516  DPQ--------SPLEPCSPNAQGASNGSNLEGEPY--NPAIFSGLSSISDSLK-RVMGES 575
              +        S +   + ++   S  + L GEP+  N  +    +S     + ++ G  
Sbjct: 671  PDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGND 730

Query: 576  FFLASPCQSFSSYFGHGKDLSGLVAKSDS-IPSTPPGADQFDVEVRGSSDEENSI----H 635
                S     +    + K    + +K D  +P+       F VE      ++ S+     
Sbjct: 731  RIDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGAT 790

Query: 636  EQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPF-----DSQSILVLMSSRNALKGTMCE 695
            EQ      T D     ++   N  E     K  F     D QSILV +S+R   KG++CE
Sbjct: 791  EQVTDGGYTNDSAVIGNQNF-NRQEQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCE 850

Query: 696  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 755
            ++H   I +Y +FD PLG+FL++NL +Q   C  C    EAH + Y H +  L I VK+L
Sbjct: 851  RAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKL 910

Query: 756  PVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 815
            P  ++LPG+ EGK+WMW RC KC    G   +T+R+++S AA GLSFGKFLEL FS+   
Sbjct: 911  P--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAA 970

Query: 816  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITET 875
             S+ + CGHSL  D L F+G G MVA FRY  + IY V++PP KL FN    Q  L  E+
Sbjct: 971  ASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYE-NQEWLQKES 1030

Query: 876  ENVYAKGMILFTEIAQFLKKIISE-----RKSSTVNDFSL-LEE---MLNEERSEFEQNI 935
            + V  K  +LF E+ + L +I ++      K ST N   L LEE   +L + + E++ ++
Sbjct: 1031 KEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSL 1090

Query: 936  QSSL--TKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEP 995
            Q  L   K G P       L +N+L   ++ +S  WD+ L    ++         +N  P
Sbjct: 1091 QQMLNVVKDGQPTIDI---LLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAP 1150

Query: 996  EPVGHE-GLESIAQTDDRNVQQDVSVDENAL----------------------------- 1055
            + +G    LE ++    +++   V++  ++L                             
Sbjct: 1151 KVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDV 1210

Query: 1056 ---HPKEIAVEHLDGESDGD------ELGLPSATE--VTEIPIMDDLSPKQLSRQGSLSN 1115
                P +  +E    E   D      ++  P+ TE       + D L    +  Q +  N
Sbjct: 1211 GSDRPPDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSEN 1270

Query: 1116 GFNR---RHSDDEDPQAG--RVLSSGDIPSSTGNLTLDKLFWTPFSEIQQMRMRDIQRSY 1175
            G  R   R +     Q    R+L S    +  G  T D+   T            + ++Y
Sbjct: 1271 GIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDE-HTTQVQLPSPSFYYSLNKNY 1330

Query: 1176 FPELKS--ISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALL 1235
                +   ++   P  + +  +   + G +L + L     ++  Y+ E +SI+A  L   
Sbjct: 1331 SLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALT-- 1390

Query: 1236 KDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLLDSLLPKTFKR 1295
                 +  +   D   + L+   S    DS + +S       S D    L S   +  + 
Sbjct: 1391 -SSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQL 1450

Query: 1296 AEHEGIIKSL------------AKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIH 1355
                  +K L             K KY V C Y  +F  LR  CCPSE  +I SL RC  
Sbjct: 1451 LHSSLYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRK 1510

Query: 1356 WNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVL 1415
            W A+GGKS  FFAK+LDDRFIIK++ +TE ESF+KF P YFKY+ ES    + + LAK+L
Sbjct: 1511 WGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKIL 1570

Query: 1416 GIYQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQ 1475
            GIYQV+ +  K GKE + +++VMENL F RN  R YDLKG+  ARYNP T+    VLLDQ
Sbjct: 1571 GIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQ 1630

Query: 1476 NFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDY 1491
            N V  M +SP++V ++AKR L+RA+WNDT+FL S++VMDYSLLVGVD E+ ELV GIID+
Sbjct: 1631 NLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDF 1690

BLAST of Cp4.1LG06g09270 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 737.6 bits (1903), Expect = 2.0e-212
Identity = 520/1435 (36.24%), Postives = 748/1435 (52.13%), Query Frame = 0

Query: 156  LWDPPEPEDPQDHVEGGMGYDDDDDDDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQR 215
            LW PPEPE+ +D  E  +  DDD D+ +  D      S+SF+         +  K++   
Sbjct: 310  LWLPPEPENEEDEREAVLS-DDDGDEGDRGDWGYLRPSNSFNEK------DFHSKDKSSG 369

Query: 216  AMQQIMNGKFKVFIGHLLKFVGVASSGE-DSENWVDIVSSLSWEAATFLKP-VVNGKAVD 275
            AM+ ++ G F+  +  LL+   +    E D E W+DI++SLSWEAAT LKP       +D
Sbjct: 370  AMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMD 429

Query: 276  PEAHVKVKCIATGTRSQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIEGMLGEAPIS-RL 335
            P  +VKVKCI  G RS+S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +L
Sbjct: 430  PGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQL 489

Query: 336  SSFNS-MDQENDFTKHVIEMIEVCTANVILVEKTTSRVIQEAMLKKGMTLVLDMKLHRLE 395
            SSF++ + QE D  K  +  I+    +++LVEK+ SR  QE +L K ++LVL++K   LE
Sbjct: 490  SSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLE 549

Query: 396  RIALCTGSPILTS-ETLMSQNTRQCDAVYFEKIVEEHAGLLEGGKRPTKTLMFIEGCPTR 455
            RI+ CTG+ I+ S + L S     CD  + EK VE H    +  K+  KTLMF +GCP  
Sbjct: 550  RISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKP 609

Query: 456  LGCTILLKGAHSDELKKIKVVVQIAVVMAFHLMLETSFLVDQRAMFATIPFGGVPATVSS 515
            LGCTILLKGAH DELKK+K V+Q  V  A+HL LETSFL D+ A    +P    P TV+ 
Sbjct: 610  LGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVAL 669

Query: 516  DPQ--------SPLEPCSPNAQGASNGSNLEGEPY--NPAIFSGLSSISDSLK-RVMGES 575
              +        S +   + ++   S  + L GEP+  N  +    +S     + ++ G  
Sbjct: 670  PDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGND 729

Query: 576  FFLASPCQSFSSYFGHGKDLSGLVAKSDS-IPSTPPGADQFDVEVRGSSDEENSI----H 635
                S     +    + K    + +K D  +P+       F VE      ++ S+     
Sbjct: 730  RIDPSERLLHNLDTVYCKPPETITSKDDGLVPTLESRQLSFHVEEPSVQKDQWSVLSGAT 789

Query: 636  EQFVPHQSTFDEGFGFHETAPNYSEDKLQKKTPF-----DSQSILVLMSSRNALKGTMCE 695
            EQ      T D     ++   N  E     K  F     D QSILV +S+R   KG++CE
Sbjct: 790  EQVTDGGYTNDSAVIGNQNF-NRQEQMESSKGDFHPSASDHQSILVSLSTRCVWKGSVCE 849

Query: 696  QSHFSHIVFYKNFDAPLGKFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQL 755
            ++H   I +Y +FD PLG+FL++NL +Q   C  C    EAH + Y H +  L I VK+L
Sbjct: 850  RAHLLRIKYYGSFDKPLGRFLRDNLFDQDQCCPSCTMPAEAHIHCYTHRQGSLTISVKKL 909

Query: 756  PVNKVLPGETEGKLWMWSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTL 815
            P  ++LPG+ EGK+WMW RC KC    G   +T+R+++S AA GLSFGKFLEL FS+   
Sbjct: 910  P--ELLPGQREGKIWMWHRCLKCPRINGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAA 969

Query: 816  PSKSSVCGHSLFGDFLYFFGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITET 875
             S+ + CGHSL  D L F+G G MVA FRY  + IY V++PP KL FN    Q  L  E+
Sbjct: 970  ASRVANCGHSLHRDCLRFYGFGRMVACFRYASINIYAVTLPPAKLYFNYE-NQEWLQKES 1029

Query: 876  ENVYAKGMILFTEIAQFLKKIISE-----RKSSTVNDFSL-LEE---MLNEERSEFEQNI 935
            + V  K  +LF E+ + L +I ++      K ST N   L LEE   +L + + E++ ++
Sbjct: 1030 KEVIKKAEVLFNEVQEALSQISAKTMGAGSKGSTPNKIKLSLEELAGLLEQRKKEYKDSL 1089

Query: 936  QSSL--TKKGNPDFPFHKYLSLNRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEP 995
            Q  L   K G P       L +N+L   ++ +S  WD+ L    ++         +N  P
Sbjct: 1090 QQMLNVVKDGQPTIDI---LLINKLRRLIIFDSYAWDECLAGAANMVRNNYLEAPKNSAP 1149

Query: 996  EPVGHE-GLESIAQTDDRNVQQDVSVDENAL----------------------------- 1055
            + +G    LE ++    +++   V++  ++L                             
Sbjct: 1150 KVMGRNVSLEKLSDEKVKSIPTHVAICNDSLLQDADYETCLNQGKSFADTSGKFAIPEDV 1209

Query: 1056 ---HPKEIAVEHLDGESDGD------ELGLPSATE--VTEIPIMDDLSPKQLSRQGSLSN 1115
                P +  +E    E   D      ++  P+ TE       + D L    +  Q +  N
Sbjct: 1210 GSDRPPDCRMEFDPSEGGKDNFVESSQVVKPAHTESQFQATDLSDTLDAAWIGEQTTSEN 1269

Query: 1116 GFNR---RHSDDEDPQAG--RVLSSGDIPSSTGNLTLDKLFWTPFSEIQQMRMRDIQRSY 1175
            G  R   R +     Q    R+L S    +  G  T D+   T            + ++Y
Sbjct: 1270 GIFRPPSRAASTNGTQIPDLRLLGSESELNFKGGPTNDE-HTTQVQLPSPSFYYSLNKNY 1329

Query: 1176 FPELKS--ISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSSIVACVLALL 1235
                +   ++   P  + +  +   + G +L + L     ++  Y+ E +SI+A  L   
Sbjct: 1330 SLNSRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALT-- 1389

Query: 1236 KDLSLETDLHNEDSKGEGLNKVPSNGSSDSDSSVSSEEHQFSSFDRLNLLDSLLPKTFKR 1295
                 +  +   D   + L+   S    DS + +S       S D    L S   +  + 
Sbjct: 1390 -SSEYKAQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQL 1449

Query: 1296 AEHEGIIKSL------------AKGKYLVNCPYFNQFRDLRRRCCPSELHYIASLSRCIH 1355
                  +K L             K KY V C Y  +F  LR  CCPSE  +I SL RC  
Sbjct: 1450 LHSSLYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRK 1509

Query: 1356 WNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQSCLAKVL 1415
            W A+GGKS  FFAK+LDDRFIIK++ +TE ESF+KF P YFKY+ ES    + + LAK+L
Sbjct: 1510 WGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKIL 1569

Query: 1416 GIYQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGEVLLDQ 1475
            GIYQV+ +  K GKE + +++VMENL F RN  R YDLKG+  ARYNP T+    VLLDQ
Sbjct: 1570 GIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQ 1629

Query: 1476 NFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVCGIIDY 1491
            N V  M +SP++V ++AKR L+RA+WNDT+FL S++VMDYSLLVGVD E+ ELV GIID+
Sbjct: 1630 NLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDF 1689