Cp4.1LG06g05010 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG06g05010
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMethyltransf_11 domain-containing protein
LocationCp4.1LG06: 3006284 .. 3010026 (+)
RNA-Seq ExpressionCp4.1LG06g05010
SyntenyCp4.1LG06g05010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATTTATTGAATGTAAATTTTATCCTCCTTGAATTTGTGAGCATTACGTAAACTTGATTCATACTTGATGGCTGCACTCCTTGGCAAGCACATGTTCGTCTTCGTTCCATTTCCCACTCGACCCTGTTTCCCACGGCGGTGCTTCTTCAAGTTCGATTCGGCCCTATCCATCTCTCATCGAGCTTCTGTTTCAACCGTTCGAGCTACTTCGGCTGCCTCCATGGAAACTAGGACCGAGCTATCGGTTTGTATTATCTCTTATCTTCTCTTGCTTGCTCTGTAAATTGAATTGTGTAGAATGGAATCTGGATGATTAGGCTCCGGTTGAAGCTGTTTGTAATTTCCTCTGCTTGGTGTTTGAGAGAATCGCAAAAGATATGAGATGATATATGCTAAATGAAATGGCCGATGGTGTGTTGAGGCGTCGTATATATATGTTTTGTTGCGAAAAATCTAGGCGGATTTGAATTGGATTGAGTTATACTGAGAGTTTGAGTGATTCAACTACGTCAATTCTAAACTTCTTTCCGAAGATATAGCCCGGCGTAACGAAACATTATCAGGAACCTCTTCAGGGTTTGAGATCATTGTTTTCAGGTAGTGAGAGAGGAAATGTAGTTTGAAGTTGGTTCTAGGTCTATTTGATTACATGTTCATTAATTCCTCATGAAAGAAGGATGTTAAAGAATACGTTGGTAAGATTAAATTAGTCAAATGTGTGTCACGTAAGAGTGACAAGCATTTATTGGGAAAATAAAAGTTGACCTAACCTGTCAACTTTACCTTTTCGAATAGCAATGTTCTTTTTTCCTAGCTCTAATGATCTTCTGGTTTTTGCGTAGGACCCTGTTTTGATTGATAAGGAAGTCAACAACATTGAGAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCAGCTGGTTCGGGACTTTCAGTGTATGTTTGAAACTGTTTTGTACCTATTTTAGCTTTTCTTATTTCTTCTATTGCGTCTTAGTAATTGATGTGTCTTTATTTATATGCTTTTGATACAGGGAGTCCACAAAGGGATATCAATTGGAATGCGGCACATGTAAAAAGTCATTCACTGGCAGTGAATCTCACTTGGACTTGACGATATCCGCTGGAATTAACAATGAAGAATCCATGCCTGCTACCACAGAGATTTTTCGGTATTTCAATGTTGTCATCTGCTTTGTATTATGAAGGGGCAGTTAATTAGCATGCTACTAAAATTTTCAAATGCAAAAGGCTTCATGGTCTTGAGAGAAATTAATTTTGTTACAAATTCCATGTGATGGATCTTTATGCTTCATGTTAAAAGGCATTTTTGTTTAGAGCTAGAAGCTCGGTAAGCCATATACACGGGGACACCATTGCTACAATAAAAATTGACGGAATCCAATATACATGGGGACATATTGGTGTACCTATCAGAGGTTTATGGAGGTCTGGGATATTTTCAGAAATATTTTTGCTTTTTGCCATGTTCGAATTAGGATTATATTGGTAGCTAGCTAGTCTTAGTTGTATTAATACAAATGAAGTTATGGGTGTTTGGTTGGAACTTGTGATACCTCTCCCTCGTTGCCTCCTTCCCCTTTTTTCTCATCTCATGTTTATCATCTTTTCACTGGATCAGGACTAGATTGGTGTCTTTTCTGTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGCAATCCTTGGATTTCCAGGCCCAGAAAAGGAGGTGATGTCAGCTCTTTTATCTGTCATGTTATTAAACTGCCTACATGGTACTTTTTATGTAATATCAGCATGTAAGTATAGGCTCAACATTAGGAATTGGATTAGTCCATTAATACCAATGCAAAGTCAGAGGCAGGTCTAGTATTTTGTTTTAGAGTGTTTTATTACTGATGAAGAGGAGGCCCTAGTGCCAACCCCTATAACAATGTTGAGGTTCGCAAAGATTAGTGATTTTAACGTGTCTCTGTTTTCACGCCTACTAGACTAGTGGTATCAGTTCAAGATGTAACCATGTTCCCAATACTAAGGACATGTTGATTTCATTGCAGTTCGAGTTTATAAAAAATTTCATCACACCAGTCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGCGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCTTCTGTTGTTGCATTGGACTACTCGGAGAACATGTTAAAGGAGTGTTATGCATTCATCAAAGAAGAGGAAAACTTCCCCAAAGAGTAAACAATATTTTCCTTTAGTTTTAGCTTTACATTAGTAGTTGTGGAATTGCTATAGTTCAAGGATCTTTGTGTTGCATATTTAGATTGTTGTTCGAGTTTTTTATCTAAATGAAAATTGTCATTCTTAGGAGCTTGGTTCTGATTAGAGCTGATATTGCTCGGCTTCCATTTGCTTCAAGTTCTATTGATGCAGTGCATGCTGGTGCTGCTTTACACTGCTGGCCTTCACCATCAGCTGCTGTGAGTATTTTTCATCTGTATCTGGGGGTTATAGGTTGTGATTTGACTTTTCATAGTTGCACTAATGAAAAACACACTGCACAAGCTGCAGTTTATTGATGGTTATTGCCTTTTTACTATAGTGATATGATTACTCATCTTCAAAGAAAATATATTTTAGTACTCTTTACTTCGCTTGTATGAGTGTTAAATGTGTATATTACTGGCACCAACATATACCTATAATTGTACCACAGATTCATCTCACTCAACTAATCAACGGATATGCTTTTGTGATTAACTGTGTCCAGAATGGTCTTGCAGGTGGCTGAAATAAGTAGAATTTTAAAACCCGGAGGAGTATTTGTTGGGAGCACTTACATAATCGATGGACCATTTTCTTTTATGCCATTTTTGGGGACTCAAATAGAGGTATATTCGCAATTTGTTTTAATTTTCCCTTCTACAACTTCTGGGATTCTCCTTGGATGCTTAGGTAGTTTGATTACTTCAGTTCATTATATATCTTTACTTTTCACTCTTTGCTTTGAAATACTTTGATGTTATTGTGAGTGAGAATATTTGCTAAAACCACCCCATCTAAACATCTAAACAGTGTGAAAGTTCTCTGTTTATAATTATTTAGAAAATCAAATAAATTACAAAAGATACCCGGAAGAGCAATAAATAGATACAAGAAAGACAAATATCTAAATATTTACCTATAATAAAATATCAAATATTCTATTATAACTAATATCCTTTTAACATAAAGTGTCAGCAAAAGCAAAAACAATCACATTAACTTAGGTTGTTAACCATTTCTTAATGCTCCAACGTTGCATCCATATCGGTTACCAGAATTCTGGTTTTTTGTTACGGTTTTTGCCTCTTACCGTAGTAGTTAATCCTTAAACTTTGTATTGTGCATCTCTCTGTGCACAGATTGCAAAGAAGATAATAGGGGGCCACATCGTTCTATCTGAAAGTGAATTAGAAGAACTCTGCATGGCCTGTGGTATGGTCGACTTTAGATGTTTAAGAAATAGGAGGTTTGTAATGTTCTCTGCTAGAAAACGCAGCTGAACATTTGGTTTGTGGTTTCCAGTCCTCTGGTTTTATTATAGTATATATATGTATATATATTTTTTTTCTTTTTTAGCATTCAGAACTTCTCAAAAGTTGATGTTTTATGTATGTTGGCTGCTGGTTGTCCTACATCTGGTTGTAATATAATTTATCCATATTTGCTTTATACCTT

mRNA sequence

CTATTTATTGAATGTAAATTTTATCCTCCTTGAATTTGTGAGCATTACGTAAACTTGATTCATACTTGATGGCTGCACTCCTTGGCAAGCACATGTTCGTCTTCGTTCCATTTCCCACTCGACCCTGTTTCCCACGGCGGTGCTTCTTCAAGTTCGATTCGGCCCTATCCATCTCTCATCGAGCTTCTGTTTCAACCGTTCGAGCTACTTCGGCTGCCTCCATGGAAACTAGGACCGAGCTATCGGACCCTGTTTTGATTGATAAGGAAGTCAACAACATTGAGAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCAGCTGGTTCGGGACTTTCAGTGGAGTCCACAAAGGGATATCAATTGGAATGCGGCACATGTAAAAAGTCATTCACTGGCAGTGAATCTCACTTGGACTTGACGATATCCGCTGGAATTAACAATGAAGAATCCATGCCTGCTACCACAGAGATTTTTCGGACTAGATTGGTGTCTTTTCTGTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGCAATCCTTGGATTTCCAGGCCCAGAAAAGGAGTTCGAGTTTATAAAAAATTTCATCACACCAGTCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGCGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCTTCTGTTGTTGCATTGGACTACTCGGAGAACATGTTAAAGGAGTGTTATGCATTCATCAAAGAAGAGGAAAACTTCCCCAAAGAGAGCTTGGTTCTGATTAGAGCTGATATTGCTCGGCTTCCATTTGCTTCAAGTTCTATTGATGCAGTGCATGCTGGTGCTGCTTTACACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATTTTAAAACCCGGAGGAGTATTTGTTGGGAGCACTTACATAATCGATGGACCATTTTCTTTTATGCCATTTTTGGGGACTCAAATAGAGATTGCAAAGAAGATAATAGGGGGCCACATCGTTCTATCTGAAAGTGAATTAGAAGAACTCTGCATGGCCTGTGGTATGGTCGACTTTAGATGTTTAAGAAATAGGAGGTTTGTAATGTTCTCTGCTAGAAAACGCAGCTGAACATTTGGTTTGTGGTTTCCAGTCCTCTGGTTTTATTATAGTATATATATGTATATATATTTTTTTTCTTTTTTAGCATTCAGAACTTCTCAAAAGTTGATGTTTTATGTATGTTGGCTGCTGGTTGTCCTACATCTGGTTGTAATATAATTTATCCATATTTGCTTTATACCTT

Coding sequence (CDS)

ATGGCTGCACTCCTTGGCAAGCACATGTTCGTCTTCGTTCCATTTCCCACTCGACCCTGTTTCCCACGGCGGTGCTTCTTCAAGTTCGATTCGGCCCTATCCATCTCTCATCGAGCTTCTGTTTCAACCGTTCGAGCTACTTCGGCTGCCTCCATGGAAACTAGGACCGAGCTATCGGACCCTGTTTTGATTGATAAGGAAGTCAACAACATTGAGAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCAGCTGGTTCGGGACTTTCAGTGGAGTCCACAAAGGGATATCAATTGGAATGCGGCACATGTAAAAAGTCATTCACTGGCAGTGAATCTCACTTGGACTTGACGATATCCGCTGGAATTAACAATGAAGAATCCATGCCTGCTACCACAGAGATTTTTCGGACTAGATTGGTGTCTTTTCTGTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGCAATCCTTGGATTTCCAGGCCCAGAAAAGGAGTTCGAGTTTATAAAAAATTTCATCACACCAGTCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGCGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCTTCTGTTGTTGCATTGGACTACTCGGAGAACATGTTAAAGGAGTGTTATGCATTCATCAAAGAAGAGGAAAACTTCCCCAAAGAGAGCTTGGTTCTGATTAGAGCTGATATTGCTCGGCTTCCATTTGCTTCAAGTTCTATTGATGCAGTGCATGCTGGTGCTGCTTTACACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATTTTAAAACCCGGAGGAGTATTTGTTGGGAGCACTTACATAATCGATGGACCATTTTCTTTTATGCCATTTTTGGGGACTCAAATAGAGATTGCAAAGAAGATAATAGGGGGCCACATCGTTCTATCTGAAAGTGAATTAGAAGAACTCTGCATGGCCTGTGGTATGGTCGACTTTAGATGTTTAAGAAATAGGAGGTTTGTAATGTTCTCTGCTAGAAAACGCAGCTGA

Protein sequence

MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSDPVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS
Homology
BLAST of Cp4.1LG06g05010 vs. ExPASy Swiss-Prot
Match: Q8LBV4 (Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g78140 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.3e-97
Identity = 183/318 (57.55%), Postives = 237/318 (74.53%), Query Frame = 0

Query: 40  SVSTVRATSAASMETRTELSDPVLIDKEVNN--IENILACSVCYGPLT-AAAGSGLSVES 99
           S S   ++++ S+ET +  +   +I+K+  N   + ILAC +CY  L   +  +GL   +
Sbjct: 39  SRSAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIESA 98

Query: 100 TKGYQLECGTCKKSFTGSESHLDLTISAGINN-EESMPATTEIFRTRLVSFLYERGWRQS 159
             G Q++C TCK+S++G+E+HLDL +++G     E MP +TE+FRT LVSFLYERGWRQ+
Sbjct: 99  ASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQN 158

Query: 160 FSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSEN 219
           F    GFPGPEKEFE  K ++ PVLGGNIIDASCGSGMFSR+F +S LFS V+ALDYSEN
Sbjct: 159 F-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSEN 218

Query: 220 MLKECYAFIKEEENFP-KESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEI 279
           ML++CY  + +EENFP KE LVL+RADIARLPF S S+DAVHAGAALHCWPSPS+AVAEI
Sbjct: 219 MLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAEI 278

Query: 280 SRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEIAKKIIGGHIVLSESELEELCMACGMVD 339
           SR+L+PGGVFV +T+I DGPFSF+PFL    +   +  G HI L+E ELE++C ACG+V+
Sbjct: 279 SRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLVN 338

Query: 340 FRCLRNRRFVMFSARKRS 353
           F  +RN  F+M SA K S
Sbjct: 339 FTRVRNGPFIMLSATKPS 355

BLAST of Cp4.1LG06g05010 vs. ExPASy Swiss-Prot
Match: Q0WPT7 (Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g41040 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 4.2e-70
Identity = 144/345 (41.74%), Postives = 215/345 (62.32%), Query Frame = 0

Query: 18  RPCFPRR-CFFKFDSALSISHRASVSTVR------ATSAASMETRTELSDPVLIDKEVNN 77
           +P  P R  FF  DS   +   ++ ++VR      A SA + ++    ++   I+ E   
Sbjct: 18  KPFLPNRSSFFSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEET- 77

Query: 78  IENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN-E 137
              + AC VCY PL     SG+++++      +CG C K+++  + +LDLT++A +++  
Sbjct: 78  --QVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYN 137

Query: 138 ESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASC 197
           E  P TTE+FR+ LVSFLYERGWRQ+F    GFPGP++EF   + +     GG ++D SC
Sbjct: 138 EVKPITTELFRSPLVSFLYERGWRQAFKR-SGFPGPDEEFRMAEEYFKEAEGGLLVDVSC 197

Query: 198 GSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKE-SLVLIRADIARLPFA 257
           GSG+FSR FA+SG +S V+ALDYSENML++C  FIK +  F    ++ ++RAD++RLPF 
Sbjct: 198 GSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFP 257

Query: 258 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGST---YIIDGPFSFMPFLGTQI 317
           S S+DAVHAGAALHCWPSP+ A+AEI R+L+ GGVFVG+T   Y    P+   PF     
Sbjct: 258 SGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIRPF----- 317

Query: 318 EIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARK 351
               +I+  +  L + E++++C +CG+ D+       F+MF+ARK
Sbjct: 318 --QSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARK 351

BLAST of Cp4.1LG06g05010 vs. ExPASy Swiss-Prot
Match: Q2YY85 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) OX=273036 GN=menG PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 7.7e-08
Identity = 36/106 (33.96%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 181 GGNIIDASCGSGMFSRIFAKS-GLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 240
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    E++ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 241 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFV 286
            D   LPF  +S D V  G  L   P    A+ E++R+LKPGG+ V
Sbjct: 110 GDAMELPFGDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of Cp4.1LG06g05010 vs. ExPASy Swiss-Prot
Match: A7X2H6 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) OX=418127 GN=menG PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.0e-07
Identity = 36/106 (33.96%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 181 GGNIIDASCGSGMFSRIFAKS-GLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 240
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    E++ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 241 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFV 286
            D   LPF  +S D V  G  L   P    A+ E++R+LKPGG+ V
Sbjct: 110 GDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of Cp4.1LG06g05010 vs. ExPASy Swiss-Prot
Match: A6U1T9 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain JH1) OX=359787 GN=menG PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.0e-07
Identity = 36/106 (33.96%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 181 GGNIIDASCGSGMFSRIFAKS-GLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 240
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    E++ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 241 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFV 286
            D   LPF  +S D V  G  L   P    A+ E++R+LKPGG+ V
Sbjct: 110 GDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of Cp4.1LG06g05010 vs. NCBI nr
Match: XP_023535731.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 697 bits (1800), Expect = 3.36e-253
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Cp4.1LG06g05010 vs. NCBI nr
Match: XP_022937076.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita moschata])

HSP 1 Score: 688 bits (1776), Expect = 1.53e-249
Identity = 347/352 (98.58%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFF+FDSALSISHR SVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFEFDSALSISHRTSVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           LGTQIEIAKK IGGHIVLSE ELEELC ACGMVDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKTIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Cp4.1LG06g05010 vs. NCBI nr
Match: XP_022976042.1 (uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxima] >XP_022976043.1 uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 687 bits (1774), Expect = 3.09e-249
Identity = 347/352 (98.58%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFP+ CFFKFDSALSISHRASVSTVRATSA SMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPQPCFFKFDSALSISHRASVSTVRATSATSMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           LGTQIEIAKKIIGGHIVLSE ELEELC ACGMVDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKIIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Cp4.1LG06g05010 vs. NCBI nr
Match: KAG7024628.1 (putative methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 687 bits (1772), Expect = 6.23e-249
Identity = 345/352 (98.01%), Postives = 347/352 (98.58%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHR SVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMP TTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           LGTQIE+AKK IGGHIVLSE ELEELC ACG+VDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 352

BLAST of Cp4.1LG06g05010 vs. NCBI nr
Match: KAG6591745.1 (putative methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667 bits (1721), Expect = 2.74e-241
Identity = 336/344 (97.67%), Postives = 338/344 (98.26%), Query Frame = 0

Query: 9   MFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSDPVLIDKEV 68
           MFVFVPFPTRPCFPRRCFFKFDSALSISHR SVSTVRATSAASMETRTELSDPVLIDKEV
Sbjct: 1   MFVFVPFPTRPCFPRRCFFKFDSALSISHRTSVSTVRATSAASMETRTELSDPVLIDKEV 60

Query: 69  NNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN 128
           NNIENILACSVCYGPLTAAA SGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN
Sbjct: 61  NNIENILACSVCYGPLTAAACSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN 120

Query: 129 EESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDAS 188
           EESMP TTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDAS
Sbjct: 121 EESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDAS 180

Query: 189 CGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFA 248
           CGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFA
Sbjct: 181 CGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFA 240

Query: 249 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEIA 308
           SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIE+A
Sbjct: 241 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEVA 300

Query: 309 KKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           KK IGGHIVLSE ELEELC ACG+VDFRCLRNRRFVMFSARKRS
Sbjct: 301 KKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 344

BLAST of Cp4.1LG06g05010 vs. ExPASy TrEMBL
Match: A0A6J1F9C0 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111443486 PE=4 SV=1)

HSP 1 Score: 688 bits (1776), Expect = 7.41e-250
Identity = 347/352 (98.58%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFPRRCFF+FDSALSISHR SVSTVRATSAASMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFEFDSALSISHRTSVSTVRATSAASMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           LGTQIEIAKK IGGHIVLSE ELEELC ACGMVDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKTIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Cp4.1LG06g05010 vs. ExPASy TrEMBL
Match: A0A6J1IKY5 (uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476562 PE=4 SV=1)

HSP 1 Score: 687 bits (1774), Expect = 1.50e-249
Identity = 347/352 (98.58%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MAALLGKHMFVFVPFPTRPCFPRRCFFKFDSALSISHRASVSTVRATSAASMETRTELSD 60
           MAALLGKHMFVFVPFPTRPCFP+ CFFKFDSALSISHRASVSTVRATSA SMETRTELSD
Sbjct: 1   MAALLGKHMFVFVPFPTRPCFPQPCFFKFDSALSISHRASVSTVRATSATSMETRTELSD 60

Query: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120
           PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL
Sbjct: 61  PVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDL 120

Query: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180
           TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL
Sbjct: 121 TISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVL 180

Query: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240
           GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA
Sbjct: 181 GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRA 240

Query: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300
           DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF
Sbjct: 241 DIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPF 300

Query: 301 LGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           LGTQIEIAKKIIGGHIVLSE ELEELC ACGMVDFRCLRNRRFVMFSARKRS
Sbjct: 301 LGTQIEIAKKIIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Cp4.1LG06g05010 vs. ExPASy TrEMBL
Match: A0A1S3CQA4 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503586 PE=4 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 1.81e-189
Identity = 274/355 (77.18%), Postives = 302/355 (85.07%), Query Frame = 0

Query: 3   ALLGKHMFVFVPFPTRPCFPR--RCFFKFDSALSISHRASVSTVRATSAASMETRTEL-- 62
           A    H+  F+P P    F    RCFF F S LSIS R S+ST+R++SA  METRT +  
Sbjct: 2   AASAMHISPFLPLPLPTQFSSFTRCFFNFHSTLSISPRLSLSTLRSSSAPPMETRTNIIP 61

Query: 63  -SDPVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESH 122
            SD V+IDKEVN I+NILACS+C GPLTAAA SGLSVEST GYQLECGTCKKSFTGSESH
Sbjct: 62  PSDSVMIDKEVNKIKNILACSICRGPLTAAADSGLSVESTNGYQLECGTCKKSFTGSESH 121

Query: 123 LDLTISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFIT 182
           LDLTI+ GIN+ ESMPA TEIFRTRLVSFLYERGWRQSFS +LGFPGPEKEFE IKNFIT
Sbjct: 122 LDLTITGGINSGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFIT 181

Query: 183 PVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVL 242
           PVLGG+IIDASCGSGMFSRIFAKSGLFSSVVALDYSENML++CY FIK+EENFP E LVL
Sbjct: 182 PVLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYGFIKQEENFPNERLVL 241

Query: 243 IRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSF 302
           IRADIARLPFASSS+DAVHAGAA+HCWPSPSA VAEISRIL+PGGVFV ST+I+DGPFSF
Sbjct: 242 IRADIARLPFASSSVDAVHAGAAVHCWPSPSAGVAEISRILRPGGVFVASTFIMDGPFSF 301

Query: 303 MPFLGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           +PFL  QIE  ++I G  I LSE ELEELC ACG+VDF+CLRNR+FVM SA KRS
Sbjct: 302 VPFLRIQIEGIQQIAGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 356

BLAST of Cp4.1LG06g05010 vs. ExPASy TrEMBL
Match: A0A6J1D0A7 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016077 PE=4 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 8.27e-188
Identity = 277/357 (77.59%), Postives = 310/357 (86.83%), Query Frame = 0

Query: 1   MAALLGKHMFV-FVPFPTRPCFPRRC-FFKFDS-ALSISHRASVSTVRATSAASMETRTE 60
           MAA++ KHM + FVP  TR CF RRC FF FDS  LSIS R S STV A+S ASM+TR +
Sbjct: 1   MAAVVAKHMLLAFVP--TRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ 60

Query: 61  LSDPVLIDKEVNNIENILACSVCYGPLTAAA-GSGLSVESTKGYQLECGTCKKSFTGSES 120
            SD  ++D+EVNNIENILACSVCYGPLTAAA GSGLSVEST GYQLECGTC+K+FTGS +
Sbjct: 61  PSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGT 120

Query: 121 HLDLTISAGINNE-ESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNF 180
           HLDLTI++G NN  + MPA+TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFE I+ F
Sbjct: 121 HLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTF 180

Query: 181 ITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESL 240
           +TP+LGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CY FI++EENFP+E+L
Sbjct: 181 MTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENL 240

Query: 241 VLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 300
           VLIRADIARLPFAS+S+DAVHAGAALHCWPSPSAAVAEISRIL+PGGVFV ST+I DGPF
Sbjct: 241 VLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPF 300

Query: 301 SFMPFLGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352
           SF+PFLGTQ E  K+I G HIVLSE ELEELC ACG+V FRC+RNR FVM SA K S
Sbjct: 301 SFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355

BLAST of Cp4.1LG06g05010 vs. ExPASy TrEMBL
Match: A0A5C7H8M2 (Methyltransf_11 domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_021984 PE=4 SV=1)

HSP 1 Score: 411 bits (1057), Expect = 1.77e-140
Identity = 203/329 (61.70%), Postives = 258/329 (78.42%), Query Frame = 0

Query: 23  RRCFFKFDSALSISHRASVSTVRATSAASMETRTELSDPVLIDKEVNNIENILACSVCYG 82
           RRCFFK  S + I +R   + +RA+S A +ET+   ++P+++DKE +  + +LAC +C+ 
Sbjct: 22  RRCFFK-TSTIPIINRKFAAKIRASSTAFVETKP--TEPIVVDKESSVNKKVLACPICFE 81

Query: 83  PLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINNE-ESMPATTEIFRT 142
           P+T      LS+ES  G  L+C TCKK+++G +SH DLT ++G  N  ESMP  TE+FRT
Sbjct: 82  PVTWIGDPCLSLESAAGSSLQCNTCKKTYSGVQSHFDLTTASGAKNYGESMPFATELFRT 141

Query: 143 RLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRIFAKS 202
             VSFLYERGWRQ+F    GFPGPEKEFE +K+++ P LGGNIIDASCGSGMFSRIFAKS
Sbjct: 142 PFVSFLYERGWRQNF-IWGGFPGPEKEFELMKDYLKPALGGNIIDASCGSGMFSRIFAKS 201

Query: 203 GLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIRADIARLPFASSSIDAVHAGAAL 262
           GLFS VVALDYSENMLK+CY F+K+EENFPKE+ +L+RADI+RLPFASSS+D VHAGAA+
Sbjct: 202 GLFSLVVALDYSENMLKQCYEFVKKEENFPKENYILVRADISRLPFASSSVDTVHAGAAI 261

Query: 263 HCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEIAKKIIGGHIVLSES 322
           HCWPSPS AVAEISR+L+PGGVFV +TYI+DGPF+F+PFL    +   +I G    +SE+
Sbjct: 262 HCWPSPSTAVAEISRVLRPGGVFVATTYILDGPFNFIPFLRPLRQNIMQISGSFTFMSEN 321

Query: 323 ELEELCMACGMVDFRCLRNRRFVMFSARK 350
           ELE+LC ACG+V F C+RNRRFVMF+A K
Sbjct: 322 ELEDLCRACGLVGFSCIRNRRFVMFTATK 346

BLAST of Cp4.1LG06g05010 vs. TAIR 10
Match: AT1G78140.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 357.1 bits (915), Expect = 1.7e-98
Identity = 183/318 (57.55%), Postives = 237/318 (74.53%), Query Frame = 0

Query: 40  SVSTVRATSAASMETRTELSDPVLIDKEVNN--IENILACSVCYGPLT-AAAGSGLSVES 99
           S S   ++++ S+ET +  +   +I+K+  N   + ILAC +CY  L   +  +GL   +
Sbjct: 39  SRSAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIESA 98

Query: 100 TKGYQLECGTCKKSFTGSESHLDLTISAGINN-EESMPATTEIFRTRLVSFLYERGWRQS 159
             G Q++C TCK+S++G+E+HLDL +++G     E MP +TE+FRT LVSFLYERGWRQ+
Sbjct: 99  ASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQN 158

Query: 160 FSAILGFPGPEKEFEFIKNFITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSEN 219
           F    GFPGPEKEFE  K ++ PVLGGNIIDASCGSGMFSR+F +S LFS V+ALDYSEN
Sbjct: 159 F-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSEN 218

Query: 220 MLKECYAFIKEEENFP-KESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEI 279
           ML++CY  + +EENFP KE LVL+RADIARLPF S S+DAVHAGAALHCWPSPS+AVAEI
Sbjct: 219 MLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAEI 278

Query: 280 SRILKPGGVFVGSTYIIDGPFSFMPFLGTQIEIAKKIIGGHIVLSESELEELCMACGMVD 339
           SR+L+PGGVFV +T+I DGPFSF+PFL    +   +  G HI L+E ELE++C ACG+V+
Sbjct: 279 SRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLVN 338

Query: 340 FRCLRNRRFVMFSARKRS 353
           F  +RN  F+M SA K S
Sbjct: 339 FTRVRNGPFIMLSATKPS 355

BLAST of Cp4.1LG06g05010 vs. TAIR 10
Match: AT2G41040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 266.5 bits (680), Expect = 2.9e-71
Identity = 144/345 (41.74%), Postives = 215/345 (62.32%), Query Frame = 0

Query: 18  RPCFPRR-CFFKFDSALSISHRASVSTVR------ATSAASMETRTELSDPVLIDKEVNN 77
           +P  P R  FF  DS   +   ++ ++VR      A SA + ++    ++   I+ E   
Sbjct: 18  KPFLPNRSSFFSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKIEIEET- 77

Query: 78  IENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHLDLTISAGINN-E 137
              + AC VCY PL     SG+++++      +CG C K+++  + +LDLT++A +++  
Sbjct: 78  --QVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADLDDYN 137

Query: 138 ESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITPVLGGNIIDASC 197
           E  P TTE+FR+ LVSFLYERGWRQ+F    GFPGP++EF   + +     GG ++D SC
Sbjct: 138 EVKPITTELFRSPLVSFLYERGWRQAFKR-SGFPGPDEEFRMAEEYFKEAEGGLLVDVSC 197

Query: 198 GSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKE-SLVLIRADIARLPFA 257
           GSG+FSR FA+SG +S V+ALDYSENML++C  FIK +  F    ++ ++RAD++RLPF 
Sbjct: 198 GSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSRLPFP 257

Query: 258 SSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGST---YIIDGPFSFMPFLGTQI 317
           S S+DAVHAGAALHCWPSP+ A+AEI R+L+ GGVFVG+T   Y    P+   PF     
Sbjct: 258 SGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTPWIIRPF----- 317

Query: 318 EIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARK 351
               +I+  +  L + E++++C +CG+ D+       F+MF+ARK
Sbjct: 318 --QSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARK 351

BLAST of Cp4.1LG06g05010 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 49.7 bits (117), Expect = 5.7e-06
Identity = 46/176 (26.14%), Postives = 74/176 (42.05%), Query Frame = 0

Query: 126 INNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKE------FEFIKNFITPV 185
           I   E +P   + F   LV+ +YE GW QSF      PG   +       E   + I   
Sbjct: 64  IETAEKVPDFVDTF-YNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVK 123

Query: 186 LGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLIR 245
            G  I+D  CG G   R  A S   ++VV +  +E  +    A +  ++        ++ 
Sbjct: 124 PGQKILDVGCGVGGPMRAIA-SHSRANVVGITINEYQVNR--ARLHNKKAGLDALCEVVC 183

Query: 246 ADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 296
            +  ++PF  +S D  ++  A    P      AEI R+LKPG ++V   ++    F
Sbjct: 184 GNFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKF 235

BLAST of Cp4.1LG06g05010 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 52/193 (26.94%), Postives = 78/193 (40.41%), Query Frame = 0

Query: 112 TGSESHLDLTISAGINNEESMPATTEIFRT--RLVSFLYERGWRQSFSAILGFPGPEKEF 171
           T  E +    +  G N EE     T++      L +  YE GW +SF     + G E   
Sbjct: 18  TAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGWGESFHFAQRWKG-ESLR 77

Query: 172 EFIK---NFITPVL----GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYA 231
           E IK   +F+   L    G  ++D  CG G   R  A+   FS+ V    + N  +    
Sbjct: 78  ESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIAR---FSNSVVTGLNNNEYQITRG 137

Query: 232 FIKEEENFPKESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGG 291
                     ++   ++AD  ++PF  +S DAV+A  A    P       EI R+LKPG 
Sbjct: 138 KELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQ 197

Query: 292 VFVGSTYIIDGPF 296
            F    + +   F
Sbjct: 198 CFAAYEWCMTDAF 206

BLAST of Cp4.1LG06g05010 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 52/193 (26.94%), Postives = 78/193 (40.41%), Query Frame = 0

Query: 112 TGSESHLDLTISAGINNEESMPATTEIFRT--RLVSFLYERGWRQSFSAILGFPGPEKEF 171
           T  E +    +  G N EE     T++      L +  YE GW +SF     + G E   
Sbjct: 18  TAVEKYEQYHVFHGGNEEERKANYTDMVNKYYDLATSFYEYGWGESFHFAQRWKG-ESLR 77

Query: 172 EFIK---NFITPVL----GGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYA 231
           E IK   +F+   L    G  ++D  CG G   R  A+   FS+ V    + N  +    
Sbjct: 78  ESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIAR---FSNSVVTGLNNNEYQITRG 137

Query: 232 FIKEEENFPKESLVLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGG 291
                     ++   ++AD  ++PF  +S DAV+A  A    P       EI R+LKPG 
Sbjct: 138 KELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQ 197

Query: 292 VFVGSTYIIDGPF 296
            F    + +   F
Sbjct: 198 CFAAYEWCMTDAF 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LBV42.3e-9757.55Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thalia... [more]
Q0WPT74.2e-7041.74Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thalia... [more]
Q2YY857.7e-0833.96Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF... [more]
A7X2H61.0e-0733.96Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain Mu3 / ATC... [more]
A6U1T91.0e-0733.96Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain JH1) OX=3... [more]
Match NameE-valueIdentityDescription
XP_023535731.13.36e-253100.00uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita pepo subsp... [more]
XP_022937076.11.53e-24998.58uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita moschata][more]
XP_022976042.13.09e-24998.58uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxim... [more]
KAG7024628.16.23e-24998.01putative methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyros... [more]
KAG6591745.12.74e-24197.67putative methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1F9C07.41e-25098.58uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata... [more]
A0A6J1IKY51.50e-24998.58uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita max... [more]
A0A1S3CQA41.81e-18977.18uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=36... [more]
A0A6J1D0A78.27e-18877.59uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charanti... [more]
A0A5C7H8M21.77e-14061.70Methyltransf_11 domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62... [more]
Match NameE-valueIdentityDescription
AT1G78140.11.7e-9857.55S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G41040.12.9e-7141.74S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G20330.15.7e-0626.14sterol methyltransferase 2 [more]
AT5G13710.11.3e-0526.94sterol methyltransferase 1 [more]
AT5G13710.21.3e-0526.94sterol methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 186..285
e-value: 5.9E-21
score: 74.9
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 139..346
e-value: 1.2E-29
score: 105.3
NoneNo IPR availablePANTHERPTHR43591:SF46OS08G0411200 PROTEINcoord: 36..351
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 36..351
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 183..291
e-value: 2.61397E-11
score: 57.825
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 144..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG06g05010.1Cp4.1LG06g05010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
molecular_function GO:0008168 methyltransferase activity