Cp4.1LG05g15440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g15440
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionUbiquinone biosynthesis O-methyltransferase, mitochondrial
LocationCp4.1LG05: 10590430 .. 10594842 (-)
RNA-Seq ExpressionCp4.1LG05g15440
SyntenyCp4.1LG05g15440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGGAATTTCAAATCATACTCGACCGAGTCCAAAATTCACCATTCCAGTTTCTCTCACTCATGGCTTCCAAATTCCGCATCAACCAGCTCCGAGCTTTAAGCTCCACCGTTTTCCATGGCATCGGAGATTGCCATTATTATTATCTGCATGCAAATCGATCTCTGCAAACTCTGTACCATTTCAGGCACTTCTCTGATGCGTCGTCTCCGCCGCTATCTCCCGCCGCTTCTTCATCTGCACCGCCTCAAAATGCAGAGCTCCAAAGCACGAATCGACCTGAGGGCATAGTAAGAAATGCTTCTGCTACCTCGTCTGTGAATCAATTCGAGCTTGCCAAATTCTCCGCCATTGCCGACACCTGGTAATCGTCGAATTCTCTAAAAAATATGCATTGTTTAGATGGTTTCTTTTTGTTGAATTATTTTCGTTGTTTAGTTGGGATGGTTTCGGAGGAAATTAGGGTTTGATGATTTGGAAATTTGAAAGTATTTAGTGTCTCACTGGGGTTCAATTCTTATTGCGAATGTACGGTGTATGATATTTCGACGGTTGGAGATTTTTGAGGCTGCTCTCCATTTTCCTGGAAATCAATCCTTTGTTACTTGGTTAGATTCTTTTACACTTACACTTACACACACACACACACACACACACACACACACACACACACACACACACACGTATCTGTGCAATGAAACTTTCACAATATTTTTTATCACTGCATAACGAATCGAAATACTTGAAGGTTCTATATTAATGGCATGTATAACCTTTATTGATACTTATTCAAAAGTTGACCAATTCACGTTAAGCTTTTGCTCTGCGAATGTATTCATGCAAGTGTCTGAGAACGGAGTTTTGATGGTTGTTCGAGATCATCATTTAATTTGATATGATCTCGATTGCATATGCTTTTTGGATCATATCTTGGTCGCACATTCTTTTGATAGATAGTGTTTCTCTCTGTCTTTGGTTTGGAGAAGTAAGAAAGAGTATTCAACCGTCATTTTGTTTGTATAGTGCTATATGTAAAGTATCTCAGTCGTTTCTTCATCAAAAAAGTATCTAATGTGCTGGGTAACTTTCGAATTCTCTATGTCCCTAATATCATCCTTGTCTTGCTATCATTTTTGTTCTTGCTCAATTCTCTTTGAATATATTTCATTCTAGAACTTAGTTCATGCCATAATAAGCATTAAAGCACTCGGTCACTTTGTGAAGACTGAAGCTGTGATTTTTAGGTGGGACTCTGAAGGGCCATTTAAGCCATTACATGCGCTGAATCCTGCAAGAGTCTCGTTCATCAGATCCACTCTTTGCCGGCATTTCAGGTACTTCTCTGGTTTATTTTCAACTGCAACTAAACCCCCCGCCCCCCACTTTTATTCTGGATCTTCCAAGCACATTTGATGAATGTTGGTTCAGTAACTATCATGATGGCTGGCTACTGACTTTAAATTAGCTCTTTTGGGTTTTTACCCAAACCATGTAGATTTGGAACCTTTTTAGTTATTTTTAGGACAAACCACCACCACCATTCTATAGCAGGATAAATGATAATTTTAATTAAGAACAACACAATGCATGGATAGCAATGTATTCTTCCTAGACCAAGGTCAACTGCATAAAAATTGGGTCCATGCAAATTCATTCTATATCTGTTTTATATTAGTTAGTAGTATTCAAAACCTGATTGCTTATTGATGGTTCTGGTTCTCTCAGTTGTGCTGAAAATATGAAACTGAGATTCTCCCTAGAATTCTAGTAAAATTATATGTATAGAATGGTATCAGACATGATATGCTTTTAATATCTAGCATGTTTTTGGTTCCTGAAATATAAAGTTTACAATTCAGTGCAAATACTTGTGAATTTGATTTTTCTTCACCTCATACTGTTCCAGGAATTCTGAATCTCTGATTGGTTAAATTTGTTGCAGGAGGGACCCTTGTTCTATTAGGCCTTTTGAAGGACTGAAAATTATTGACGTTGGTTGTGGTGGTGGAATTCTCTCTGAGGTCTCTAGTTTTCTTTTGGTCTTGCTGTATTCTTATTACAAAGGGAATTTTATTGTCCAGTACAACATGGATGTTTTTTTTTCTTCATAGATACCATTTGTCTAGTTCCCGGAGTTTGGGGTTTTCCAAATTCATTTCCCCAATTTATATCTGTTAACTAAAGCAATAATTCTTAAATCTATTCTTTGGAAATGAGTCGATAATTATTAATAATAGATTTACAAGTTGTCAACTTATTAGTACAAAAGCCACACAATTTCTCTGTCAGCATNCCCCTCCTCCTCCTCCTCCTCCTCCTCTTTTGGTCAAATATAAAGACAAAATTGAAGTATGACATTCAATTGAGGTTAGAGGAGAGCAAAGACCACCCATGTGTGCTGTAAAATAATATTCTCCCTCAATACAAACTTTTCTGAAGCTCCAGTCCTGTGGCTGGTAGCTGAGCTGTCCTGTCCCATATTGTCAAAAGAAATAAGATCAGTCCAAATTAGAGTTAAAAAACTGAGCTGTTCACTAATTTGTAGGCTTAAAACCATACTCTTTAGTGCCATCCTGCTCTCTTAGGTTAAATTATTTTTCTTGGTATGTGTTAACTTTTGGAAGATGTTCTTGATCCCATGTTCAATATCTTTCTTTTTCTGTTATTTTAGCCTTTAGCTCGAATGGGAGCAACTGTGACAGGAATTGACGCTGTGGATAAAAATATCAAGATTGCCCGTGTGCATGCGGTATGGTTATTATTCTTGAAGTCAAACAAATTGTTTATTTGGCAAGATCTATGTAGAATTTAACTTGGTGGTTTCTGGACATACTTGTATGTGCAGGACCTGGATCCTGTGACATCAACAATTGAATATTGTTGTACCACAGCTGGTACGTGCAGAAACTTGATTTTCTCATCATGACCTCTTTCCTGCAGTATCAAACCTCTGTTATTTTTATATCTATTATGTGTTTGAACTTCAGATGATTTTGCCATGTTGTATAATTGAAAAATCTAGCCAAATTACCTTCAACTCCCTTTGAGAGTGCTATAAAATATACCAGATTCCTATCTTTTGGTTGACTACCTCCAACCTACTTGTTCTCTTCCAAAGGCATTACACCTTCTTCTAAGTAAGAGAGAGTTGTGTTACACTATTCTTATGACGGAAAAAGAGTGTATCATGCTCTTCTTACGGATACACGTATCTTCCACCTTTATTGTTTATCCTTGTATGCTCAAATTATTTGTTTCACTTAAATCACTTTAATTTTTCATTGAGAAATCCTATTGCTTGTTTATCATTGAATATTGAATATTCTACAATACTACTTTGTCACAGAAAAGCTGGTAGAGCAGCAAAAAGAGTTTGATGCTGTCATCGCTTCGGAGGTTTGCTCGCACTCTCACTCAACATGTGGAAAATTGACTAGACATTATTTATCAAAATTTCTTCATAAACCTGGTTTCAAATGCAACCATAACGTAGGCTGTACCCCTTTCGGGCCACTCCTTTTATTTGTTAGCCTGATCATACCCTTAGACTGCAATTAACTGTTAGATACATATCCAAGCTCTTCTAAGCAAAACCTTTCAATAGGTAATTGAGCATGTGGCAGATCCTGCAGGATTTTGCCAGTCCTTGGCAGCATTGACAGTGCCAAATGGAGCTACAGTTGTTTCTACTATTAATCGTTCTATGAGGTCATTCGCAACTGCCATTGTTGCAGCAGAATACATTCTTCGTTGGGTATACTATTATCAACTTTCTTTTTTTCTAATCTAATTTCTCTTGAGACGACACAGCTACTTTTATTATATTTTCTTATACTTACAGCTTCCTAGAGGTACGCATCAATGGTCAAGCTTACTTACCCCTGAAGAATTGGTTCTGATATTACAACGGGCCTCTATTTCAGTAAGACTAATGCTTAAATATTCATATGTTTATGTTCTTACTAATTCTTTCTCCAACATGCACTGTTTTCTATGTTCAAACACCCAATTCATGTATGATTGTTTTTTCCCTTAGGTGGAAGAGATAGCCGGATTTGTGTATAATCCCTTAACCGGCCAATGGTCCCTTTCAGATGATACCAGTGTAAATTACATCGCATTTGGTACAAAAGTCAGCCAGAAGACAGACTGACGAAGGTGTAATCTTAGTTCTCACGCAATAGGTATTATTTCTGTCGCTAAATTAATTTTGCCCTGAAAACTGAATGGTTACTCTCAATCAGCTTCCCTCTAAACATTCCATAAAGCTTCTTTTTCTTTGTTCCAATTAGACCTTTCAATCTCGTATAACATAAACCAAATTTGTTTGAGGTTTATGATCTTGGCAGACTCCTCATTTTGAGCACAAAAGGGAAGAAAAAATTGTTGTGAATTGCATTTGGTTGTGAATATT

mRNA sequence

TGTGGAATTTCAAATCATACTCGACCGAGTCCAAAATTCACCATTCCAGTTTCTCTCACTCATGGCTTCCAAATTCCGCATCAACCAGCTCCGAGCTTTAAGCTCCACCGTTTTCCATGGCATCGGAGATTGCCATTATTATTATCTGCATGCAAATCGATCTCTGCAAACTCTGTACCATTTCAGGCACTTCTCTGATGCGTCGTCTCCGCCGCTATCTCCCGCCGCTTCTTCATCTGCACCGCCTCAAAATGCAGAGCTCCAAAGCACGAATCGACCTGAGGGCATAGTAAGAAATGCTTCTGCTACCTCGTCTGTGAATCAATTCGAGCTTGCCAAATTCTCCGCCATTGCCGACACCTGGTGGGACTCTGAAGGGCCATTTAAGCCATTACATGCGCTGAATCCTGCAAGAGTCTCGTTCATCAGATCCACTCTTTGCCGGCATTTCAGGAGGGACCCTTGTTCTATTAGGCCTTTTGAAGGACTGAAAATTATTGACGTTGGTTGTGGTGGTGGAATTCTCTCTGAGCCTTTAGCTCGAATGGGAGCAACTGTGACAGGAATTGACGCTGTGGATAAAAATATCAAGATTGCCCGTGTGCATGCGGACCTGGATCCTGTGACATCAACAATTGAATATTGTTGTACCACAGCTGAAAAGCTGGTAGAGCAGCAAAAAGAGTTTGATGCTGTCATCGCTTCGGAGGTAATTGAGCATGTGGCAGATCCTGCAGGATTTTGCCAGTCCTTGGCAGCATTGACAGTGCCAAATGGAGCTACAGTTGTTTCTACTATTAATCGTTCTATGAGGTCATTCGCAACTGCCATTGTTGCAGCAGAATACATTCTTCGTTGGCTTCCTAGAGGTACGCATCAATGGTCAAGCTTACTTACCCCTGAAGAATTGGTTCTGATATTACAACGGGCCTCTATTTCAGTGGAAGAGATAGCCGGATTTGTGTATAATCCCTTAACCGGCCAATGGTCCCTTTCAGATGATACCAGTGTAAATTACATCGCATTTGGTACAAAAGTCAGCCAGAAGACAGACTGACGAAGGTGTAATCTTAGTTCTCACGCAATAGGTATTATTTCTGTCGCTAAATTAATTTTGCCCTGAAAACTGAATGGTTACTCTCAATCAGCTTCCCTCTAAACATTCCATAAAGCTTCTTTTTCTTTGTTCCAATTAGACCTTTCAATCTCGTATAACATAAACCAAATTTGTTTGAGGTTTATGATCTTGGCAGACTCCTCATTTTGAGCACAAAAGGGAAGAAAAAATTGTTGTGAATTGCATTTGGTTGTGAATATT

Coding sequence (CDS)

ATGGCTTCCAAATTCCGCATCAACCAGCTCCGAGCTTTAAGCTCCACCGTTTTCCATGGCATCGGAGATTGCCATTATTATTATCTGCATGCAAATCGATCTCTGCAAACTCTGTACCATTTCAGGCACTTCTCTGATGCGTCGTCTCCGCCGCTATCTCCCGCCGCTTCTTCATCTGCACCGCCTCAAAATGCAGAGCTCCAAAGCACGAATCGACCTGAGGGCATAGTAAGAAATGCTTCTGCTACCTCGTCTGTGAATCAATTCGAGCTTGCCAAATTCTCCGCCATTGCCGACACCTGGTGGGACTCTGAAGGGCCATTTAAGCCATTACATGCGCTGAATCCTGCAAGAGTCTCGTTCATCAGATCCACTCTTTGCCGGCATTTCAGGAGGGACCCTTGTTCTATTAGGCCTTTTGAAGGACTGAAAATTATTGACGTTGGTTGTGGTGGTGGAATTCTCTCTGAGCCTTTAGCTCGAATGGGAGCAACTGTGACAGGAATTGACGCTGTGGATAAAAATATCAAGATTGCCCGTGTGCATGCGGACCTGGATCCTGTGACATCAACAATTGAATATTGTTGTACCACAGCTGAAAAGCTGGTAGAGCAGCAAAAAGAGTTTGATGCTGTCATCGCTTCGGAGGTAATTGAGCATGTGGCAGATCCTGCAGGATTTTGCCAGTCCTTGGCAGCATTGACAGTGCCAAATGGAGCTACAGTTGTTTCTACTATTAATCGTTCTATGAGGTCATTCGCAACTGCCATTGTTGCAGCAGAATACATTCTTCGTTGGCTTCCTAGAGGTACGCATCAATGGTCAAGCTTACTTACCCCTGAAGAATTGGTTCTGATATTACAACGGGCCTCTATTTCAGTGGAAGAGATAGCCGGATTTGTGTATAATCCCTTAACCGGCCAATGGTCCCTTTCAGATGATACCAGTGTAAATTACATCGCATTTGGTACAAAAGTCAGCCAGAAGACAGACTGA

Protein sequence

MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSAPPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVSFIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGATVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGFVYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD
Homology
BLAST of Cp4.1LG05g15440 vs. ExPASy Swiss-Prot
Match: O49354 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=COQ3 PE=1 SV=2)

HSP 1 Score: 409.5 bits (1051), Expect = 3.7e-113
Identity = 199/289 (68.86%), Postives = 247/289 (85.47%), Query Frame = 0

Query: 38  LYHFRHFSDASSPPLSPAASSSAPP-QNAELQSTNRPEGIVRNASATSSVNQFELAKFSA 97
           L H R FS + +   + + SSS P  Q  E +++N+     R+ S+T+S+N+ ELAKFSA
Sbjct: 31  LLHQRLFSTSDTDASAASFSSSHPKIQTLEGKASNKS----RSTSSTTSLNEDELAKFSA 90

Query: 98  IADTWWDSEGPFKPLHALNPARVSFIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILS 157
           IADTWW SEGPFKPLH +NP R++FIRSTLCRHF +DP S +PFEGLK ID+GCGGG+LS
Sbjct: 91  IADTWWHSEGPFKPLHQMNPTRLAFIRSTLCRHFSKDPSSAKPFEGLKFIDIGCGGGLLS 150

Query: 158 EPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASE 217
           EPLARMGATVTG+DAVDKN+KIAR+HAD+DPVTSTIEY CTTAEKL ++ ++FDAV++ E
Sbjct: 151 EPLARMGATVTGVDAVDKNVKIARLHADMDPVTSTIEYLCTTAEKLADEGRKFDAVLSLE 210

Query: 218 VIEHVADPAGFCQSLAALTVPNGATVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSS 277
           VIEHVA+PA FC+SL+ALT+PNGATV+STINR+MR++A+ IV AEYILRWLP+GTHQWSS
Sbjct: 211 VIEHVANPAEFCKSLSALTIPNGATVLSTINRTMRAYASTIVGAEYILRWLPKGTHQWSS 270

Query: 278 LLTPEELVLILQRASISVEEIAGFVYNPLTGQWSLSDDTSVNYIAFGTK 326
            +TPEE+ +ILQRAS+ V+EIAGFVYNP+TG+W LSDD SVNYIA+GTK
Sbjct: 271 FVTPEEMSMILQRASVDVKEIAGFVYNPITGRWLLSDDISVNYIAYGTK 315

BLAST of Cp4.1LG05g15440 vs. ExPASy Swiss-Prot
Match: Q2RWE9 (Ubiquinone biosynthesis O-methyltransferase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=ubiG PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.4e-67
Identity = 124/249 (49.80%), Postives = 164/249 (65.86%), Query Frame = 0

Query: 79  NASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVSFIRSTLCRHFRRDPCSIR 138
           +A+ + + +  ELAKFSA+AD WWD EG FKPLH  NP R++F+R     HF RD  + R
Sbjct: 2   SATTSGTASAAELAKFSAMADAWWDPEGDFKPLHKFNPVRIAFLRDHFAAHFGRDIEAPR 61

Query: 139 PFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEYCCTT 198
           PFEGL ++D+GCGGG+L EP AR+G  VTGIDA ++NI  A VHA+   +  T  Y C  
Sbjct: 62  PFEGLSLLDIGCGGGLLCEPFARLGFAVTGIDAAERNIGTASVHAERAGLPLT--YRCAM 121

Query: 199 AEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGATVVSTINRSMRSFATAIV 258
            E LV + K FDAV+  EV+EHVAD   F  S+  L  P GA   +T+NR+++S A A V
Sbjct: 122 PEDLVAEGKTFDAVLTMEVVEHVADVRLFLDSVGQLCRPGGAVGAATLNRTLKSLALAKV 181

Query: 259 AAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGFVYNPLTGQWSLSDDTSVN 318
            AEY+LRWLPRGTH W   + P EL   L+ A +SV+ IAG  ++P +G WS + D SVN
Sbjct: 182 GAEYVLRWLPRGTHDWRKFMKPSELTAGLREAGLSVDAIAGMTFDPFSGTWSQTTDVSVN 241

Query: 319 YIAFGTKVS 328
           Y+ F T+ +
Sbjct: 242 YMLFATRAA 248

BLAST of Cp4.1LG05g15440 vs. ExPASy Swiss-Prot
Match: B8IUB0 (Ubiquinone biosynthesis O-methyltransferase OS=Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060) OX=460265 GN=ubiG PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.5e-66
Identity = 120/236 (50.85%), Postives = 159/236 (67.37%), Query Frame = 0

Query: 85  SVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVSFIRSTLCRHFRRDPCSIRPFEGLK 144
           S+++ E+A+F  IA TWWD  GP + LH  NP R+++IR T+CRHF RDP    P E L 
Sbjct: 8   SIDRDEVARFERIAATWWDEAGPMRVLHRFNPVRITYIRDTVCRHFGRDPRRPLPLEALS 67

Query: 145 IIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEYCCTTAEKLVE 204
           +ID+GCGGGILSEPLAR+GATVTG+D    NI++A+ HA    V   ++Y   T E +VE
Sbjct: 68  LIDIGCGGGILSEPLARLGATVTGLDPAPTNIRVAQAHAAEAGV--PVDYRGQTIEAVVE 127

Query: 205 QQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGATVVSTINRSMRSFATAIVAAEYIL 264
             + FD V+A EV+EHV D   F ++  A   P G    +T+NR+MRSFA AIV AEY+L
Sbjct: 128 AGERFDVVLAMEVVEHVVDMPAFVRTACAAVKPGGLFFAATLNRTMRSFALAIVGAEYVL 187

Query: 265 RWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGFVYNPLTGQWSLSDDTSVNYI 321
            WLPRGTH W   +TP EL   ++ A ++V +  G VYNPL G+W++S DT VNY+
Sbjct: 188 GWLPRGTHDWEKFVTPAELTGAVESAGLTVIDTTGVVYNPLGGRWAMSRDTGVNYM 241

BLAST of Cp4.1LG05g15440 vs. ExPASy Swiss-Prot
Match: A8HVC4 (Ubiquinone biosynthesis O-methyltransferase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571) OX=438753 GN=ubiG PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 9.9e-66
Identity = 117/243 (48.15%), Postives = 164/243 (67.49%), Query Frame = 0

Query: 81  SATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVSFIRSTLCRHFRRDPCSIRPF 140
           +A ++++  E+A+F A+   WWD +G   PLHA+NP R+ F+R  L RHF  D  S+RP 
Sbjct: 4   TANATIDPSEVARFDALGAQWWDPKGKMAPLHAINPVRLGFVRDVLVRHFGHDARSLRPL 63

Query: 141 EGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEYCCTTAE 200
           +GL+I+D+GCGGG+LSEPLARMGA + G+D    NI +A+ HAD   V   ++Y   TAE
Sbjct: 64  KGLRILDIGCGGGLLSEPLARMGADMVGVDPAPGNIVVAQSHADEAGV--RVDYRQGTAE 123

Query: 201 KLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGATVVSTINRSMRSFATAIVAA 260
           +L +  + FD V+A EV+EHVAD   F +    +  PNG  +VST+NR+++SFA AIV A
Sbjct: 124 ELADAGERFDVVLALEVVEHVADVGLFLRRAGEMVNPNGIMIVSTLNRTVKSFALAIVGA 183

Query: 261 EYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGFVYNPLTGQWSLSDDTSVNYI 320
           EY+LRWLPRGTH+W   +TP EL   +  A   + E+AG V++PL G+W ++ DT VNY 
Sbjct: 184 EYVLRWLPRGTHRWDKFITPAELEAEMGAAGFEMSELAGMVFDPLRGEWKIAADTDVNYF 243

Query: 321 AFG 324
             G
Sbjct: 244 LVG 244

BLAST of Cp4.1LG05g15440 vs. ExPASy Swiss-Prot
Match: B0UAV0 (Ubiquinone biosynthesis O-methyltransferase OS=Methylobacterium sp. (strain 4-46) OX=426117 GN=ubiG PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 117/242 (48.35%), Postives = 158/242 (65.29%), Query Frame = 0

Query: 79  NASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVSFIRSTLCRHFRRDPCSIR 138
           +A    S++  E+A+F  IA TWWD  GP + LH  NP R+++IR   CRH  RDP    
Sbjct: 2   SAPTGPSIDAREVARFERIAATWWDEAGPMRVLHRFNPVRLAYIRDAACRHHGRDPLGPE 61

Query: 139 PFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEYCCTT 198
           P  GL ++D+GCGGG+LSEPLAR+GA VTG+D    NI++A+ HA+   V   ++Y   T
Sbjct: 62  PLAGLTLVDIGCGGGVLSEPLARLGARVTGLDPAPTNIRVAKAHAEEAGV--PVDYRPQT 121

Query: 199 AEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGATVVSTINRSMRSFATAIV 258
            E +V   + FD V+A EV+EHVAD A F ++  A   P G    +TINR+MRSFA AIV
Sbjct: 122 IESVVAAGERFDLVVAMEVVEHVADMASFVRTACAAVRPGGLFFAATINRTMRSFALAIV 181

Query: 259 AAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGFVYNPLTGQWSLSDDTSVN 318
            AEY+L WLPRGTH W   +TPEEL   ++   + V +  G VY+PL+G+WS + DT+VN
Sbjct: 182 GAEYVLGWLPRGTHDWEKFVTPEELARAVESGGLVVADTTGVVYHPLSGRWSAARDTAVN 241

Query: 319 YI 321
           Y+
Sbjct: 242 YM 241

BLAST of Cp4.1LG05g15440 vs. NCBI nr
Match: XP_023532537.1 (ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659 bits (1700), Expect = 1.15e-238
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA
Sbjct: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS
Sbjct: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA
Sbjct: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF
Sbjct: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331

BLAST of Cp4.1LG05g15440 vs. NCBI nr
Match: KAG7034867.1 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 641 bits (1654), Expect = 1.14e-231
Identity = 326/331 (98.49%), Postives = 326/331 (98.49%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MASKFRINQLRALSSTVFHGIGDCHYY LHANRS QTLYHFR FSDASSPPLSPAASSSA
Sbjct: 1   MASKFRINQLRALSSTVFHGIGDCHYY-LHANRSPQTLYHFRRFSDASSPPLSPAASSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNAELQSTNRPE IVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS
Sbjct: 61  PPQNAELQSTNRPEEIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA
Sbjct: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           TVVSTINRSMRSFA AIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF
Sbjct: 241 TVVSTINRSMRSFAVAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 330

BLAST of Cp4.1LG05g15440 vs. NCBI nr
Match: KAG6604738.1 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 640 bits (1651), Expect = 3.25e-231
Identity = 325/331 (98.19%), Postives = 326/331 (98.49%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MASKFRINQLRAL STVFHGIGDCHYY LHANRS QTLYHFR FSDASSPPLSPAASSSA
Sbjct: 1   MASKFRINQLRALCSTVFHGIGDCHYY-LHANRSPQTLYHFRRFSDASSPPLSPAASSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNAELQSTNRP+ IVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS
Sbjct: 61  PPQNAELQSTNRPDEIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA
Sbjct: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF
Sbjct: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 330

BLAST of Cp4.1LG05g15440 vs. NCBI nr
Match: XP_022946983.1 (ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 637 bits (1642), Expect = 7.66e-230
Identity = 324/331 (97.89%), Postives = 324/331 (97.89%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MA KFRINQLRALSSTVFHGIGDCHYY LHANRS QTLYHFR FSDASSPPLSPAASSSA
Sbjct: 1   MAPKFRINQLRALSSTVFHGIGDCHYY-LHANRSPQTLYHFRRFSDASSPPLSPAASSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNAELQSTNRPE IVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS
Sbjct: 61  PPQNAELQSTNRPEEIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA
Sbjct: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLL PEELVLILQRASISVEEIAGF
Sbjct: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLIPEELVLILQRASISVEEIAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFGTKVSQ TD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQTTD 330

BLAST of Cp4.1LG05g15440 vs. NCBI nr
Match: XP_022970767.1 (ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 634 bits (1636), Expect = 6.29e-229
Identity = 323/331 (97.58%), Postives = 325/331 (98.19%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MASKFRINQLRALSSTVFHGIG  HYY LHANRS QTLYHFRHFSDASSPPLSPAASSSA
Sbjct: 1   MASKFRINQLRALSSTVFHGIGVRHYY-LHANRSPQTLYHFRHFSDASSPPLSPAASSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNAELQS NRPE IVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS
Sbjct: 61  PPQNAELQSVNRPEDIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADP+GFCQSLAALTVPNGA
Sbjct: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPSGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEE+AGF
Sbjct: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEMAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 330

BLAST of Cp4.1LG05g15440 vs. ExPASy TrEMBL
Match: A0A6J1G5C7 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111450999 PE=3 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 3.71e-230
Identity = 324/331 (97.89%), Postives = 324/331 (97.89%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MA KFRINQLRALSSTVFHGIGDCHYY LHANRS QTLYHFR FSDASSPPLSPAASSSA
Sbjct: 1   MAPKFRINQLRALSSTVFHGIGDCHYY-LHANRSPQTLYHFRRFSDASSPPLSPAASSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNAELQSTNRPE IVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS
Sbjct: 61  PPQNAELQSTNRPEEIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA
Sbjct: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLL PEELVLILQRASISVEEIAGF
Sbjct: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLIPEELVLILQRASISVEEIAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFGTKVSQ TD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQTTD 330

BLAST of Cp4.1LG05g15440 vs. ExPASy TrEMBL
Match: A0A6J1I023 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111469658 PE=3 SV=1)

HSP 1 Score: 634 bits (1636), Expect = 3.05e-229
Identity = 323/331 (97.58%), Postives = 325/331 (98.19%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MASKFRINQLRALSSTVFHGIG  HYY LHANRS QTLYHFRHFSDASSPPLSPAASSSA
Sbjct: 1   MASKFRINQLRALSSTVFHGIGVRHYY-LHANRSPQTLYHFRHFSDASSPPLSPAASSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNAELQS NRPE IVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS
Sbjct: 61  PPQNAELQSVNRPEDIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADP+GFCQSLAALTVPNGA
Sbjct: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPSGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEE+AGF
Sbjct: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEMAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 330

BLAST of Cp4.1LG05g15440 vs. ExPASy TrEMBL
Match: A0A6J1D8W9 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111018122 PE=3 SV=1)

HSP 1 Score: 562 bits (1448), Expect = 1.30e-200
Identity = 280/331 (84.59%), Postives = 304/331 (91.84%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MAS FRIN LRAL STV+HGIG+ HY  L+ANR+ Q LYHFRHFSDA SP ++ A SSSA
Sbjct: 1   MASNFRINLLRALCSTVYHGIGNSHY--LNANRAPQALYHFRHFSDAPSPTIATAGSSSA 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQNA +QST RP+ IVRNASA SSVNQ ELAKFSAIADTWWDSEGPFKPLH +NP R+S
Sbjct: 61  PPQNAAVQSTARPKEIVRNASAASSVNQLELAKFSAIADTWWDSEGPFKPLHVMNPTRLS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDP S+RPFEGL I DVGCGGGILSEPLARMGA VTGIDAV+KNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPYSVRPFEGLNIADVGCGGGILSEPLARMGANVTGIDAVEKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           +HADLDPVTSTIEYCCTTAEKLVEQ+K+FDAVIA EVIEHVADPAGFCQSLAALTVPNGA
Sbjct: 181 LHADLDPVTSTIEYCCTTAEKLVEQEKKFDAVIALEVIEHVADPAGFCQSLAALTVPNGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           T++STINRSMR+FATAIVAAEYIL WLP+GTHQWSS LTPEELVLILQR+SISVEE+AGF
Sbjct: 241 TIISTINRSMRAFATAIVAAEYILHWLPKGTHQWSSFLTPEELVLILQRSSISVEEMAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWS+SDDTSVNYIAFGTK SQ+T+
Sbjct: 301 VYNPLTGQWSVSDDTSVNYIAFGTKSSQQTE 329

BLAST of Cp4.1LG05g15440 vs. ExPASy TrEMBL
Match: A0A1S3C334 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103496335 PE=3 SV=1)

HSP 1 Score: 546 bits (1408), Expect = 1.39e-194
Identity = 281/331 (84.89%), Postives = 297/331 (89.73%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MASKFRI+QLRAL STVFHGIGD       + R+ Q  Y  RHFSDASSPPLSPA SS  
Sbjct: 1   MASKFRIDQLRALGSTVFHGIGDSL-----SIRASQNHY-IRHFSDASSPPLSPAGSSLP 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQN ELQ+  +PE +  NAS TSSVNQ ELAKFSAI++TWWD+EGPFKPLHALNP RVS
Sbjct: 61  PPQNVELQTAPQPEKMNINASTTSSVNQLELAKFSAISETWWDAEGPFKPLHALNPTRVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPCS+RPFEGLK IDVGCGGGILSEPLARMGATVTGID VD NIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCSVRPFEGLKFIDVGCGGGILSEPLARMGATVTGIDMVDNNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHADLDPVTSTIEY CTTAE LVEQQKEFDAVIASEVIEHVA+PAGFCQSLAALTV NGA
Sbjct: 181 VHADLDPVTSTIEYYCTTAENLVEQQKEFDAVIASEVIEHVANPAGFCQSLAALTVANGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           T+VSTINRSMR+FATAIVAAEYILRWLP GTHQWSSLLTPEELVLILQRASISVEE+AGF
Sbjct: 241 TIVSTINRSMRAFATAIVAAEYILRWLPIGTHQWSSLLTPEELVLILQRASISVEEMAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFG K+SQ+TD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGKKISQQTD 325

BLAST of Cp4.1LG05g15440 vs. ExPASy TrEMBL
Match: A0A0A0KBI6 (Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucumis sativus OX=3659 GN=COQ3 PE=3 SV=1)

HSP 1 Score: 541 bits (1393), Expect = 2.69e-192
Identity = 278/331 (83.99%), Postives = 294/331 (88.82%), Query Frame = 0

Query: 1   MASKFRINQLRALSSTVFHGIGDCHYYYLHANRSLQTLYHFRHFSDASSPPLSPAASSSA 60
           MASK RI+QLRALSSTVFHGIGD       + R+ Q  +H RHFSD SSP LSPA SSS 
Sbjct: 1   MASKLRIDQLRALSSTVFHGIGDSL-----SIRASQN-HHIRHFSDVSSPTLSPAGSSSP 60

Query: 61  PPQNAELQSTNRPEGIVRNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVS 120
           PPQN EL++  +PE +  NAS TSSVNQ ELAKFSAI+DTWWD+EGPFKPLHALNP RVS
Sbjct: 61  PPQNVELKTAPQPEKMNINASTTSSVNQIELAKFSAISDTWWDAEGPFKPLHALNPTRVS 120

Query: 121 FIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILSEPLARMGATVTGIDAVDKNIKIAR 180
           FIRSTLCRHFRRDPC +RPFEGL  IDVGCGGGILSEPLARMGATVTGID VDKNIKIAR
Sbjct: 121 FIRSTLCRHFRRDPCLVRPFEGLNFIDVGCGGGILSEPLARMGATVTGIDMVDKNIKIAR 180

Query: 181 VHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVPNGA 240
           VHAD DPVTSTIEY CTTAE LVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTV NGA
Sbjct: 181 VHADQDPVTSTIEYYCTTAENLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVANGA 240

Query: 241 TVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSSLLTPEELVLILQRASISVEEIAGF 300
           T+VSTINRSMR+FA AIVAAEYILRWLP GTHQWSSLLTPEELVLILQRASISVEE+AGF
Sbjct: 241 TIVSTINRSMRAFAAAIVAAEYILRWLPIGTHQWSSLLTPEELVLILQRASISVEEMAGF 300

Query: 301 VYNPLTGQWSLSDDTSVNYIAFGTKVSQKTD 331
           VYNPLTGQWSLSDDTSVNYIAFG K+SQ+TD
Sbjct: 301 VYNPLTGQWSLSDDTSVNYIAFGKKISQQTD 325

BLAST of Cp4.1LG05g15440 vs. TAIR 10
Match: AT2G30920.1 (coenzyme Q 3 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.6e-114
Identity = 199/289 (68.86%), Postives = 247/289 (85.47%), Query Frame = 0

Query: 38  LYHFRHFSDASSPPLSPAASSSAPP-QNAELQSTNRPEGIVRNASATSSVNQFELAKFSA 97
           L H R FS + +   + + SSS P  Q  E +++N+     R+ S+T+S+N+ ELAKFSA
Sbjct: 31  LLHQRLFSTSDTDASAASFSSSHPKIQTLEGKASNKS----RSTSSTTSLNEDELAKFSA 90

Query: 98  IADTWWDSEGPFKPLHALNPARVSFIRSTLCRHFRRDPCSIRPFEGLKIIDVGCGGGILS 157
           IADTWW SEGPFKPLH +NP R++FIRSTLCRHF +DP S +PFEGLK ID+GCGGG+LS
Sbjct: 91  IADTWWHSEGPFKPLHQMNPTRLAFIRSTLCRHFSKDPSSAKPFEGLKFIDIGCGGGLLS 150

Query: 158 EPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEYCCTTAEKLVEQQKEFDAVIASE 217
           EPLARMGATVTG+DAVDKN+KIAR+HAD+DPVTSTIEY CTTAEKL ++ ++FDAV++ E
Sbjct: 151 EPLARMGATVTGVDAVDKNVKIARLHADMDPVTSTIEYLCTTAEKLADEGRKFDAVLSLE 210

Query: 218 VIEHVADPAGFCQSLAALTVPNGATVVSTINRSMRSFATAIVAAEYILRWLPRGTHQWSS 277
           VIEHVA+PA FC+SL+ALT+PNGATV+STINR+MR++A+ IV AEYILRWLP+GTHQWSS
Sbjct: 211 VIEHVANPAEFCKSLSALTIPNGATVLSTINRTMRAYASTIVGAEYILRWLPKGTHQWSS 270

Query: 278 LLTPEELVLILQRASISVEEIAGFVYNPLTGQWSLSDDTSVNYIAFGTK 326
            +TPEE+ +ILQRAS+ V+EIAGFVYNP+TG+W LSDD SVNYIA+GTK
Sbjct: 271 FVTPEEMSMILQRASVDVKEIAGFVYNPITGRWLLSDDISVNYIAYGTK 315

BLAST of Cp4.1LG05g15440 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 42.7 bits (99), Expect = 6.5e-04
Identity = 44/163 (26.99%), Postives = 66/163 (40.49%), Query Frame = 0

Query: 78  RNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVSFIRSTLCR--HFRRDPC 137
           R A+ T  VN++     S     W +S       H     +   +R ++ R  HF     
Sbjct: 37  RKANYTDMVNKYYDLATSFYEYGWGES------FHFAQRWKGESLRESIKRHEHFLALQL 96

Query: 138 SIRPFEGLKIIDVGCG-GGILSEPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEY 197
            I+P  G K++DVGCG GG L E      + VTG++  +  I   +    L  V  T  +
Sbjct: 97  GIQP--GQKVLDVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNF 156

Query: 198 CCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVP 238
                 K+   +  FDAV A E   H  D  G  + +  +  P
Sbjct: 157 VKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKP 191

BLAST of Cp4.1LG05g15440 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 42.7 bits (99), Expect = 6.5e-04
Identity = 44/163 (26.99%), Postives = 66/163 (40.49%), Query Frame = 0

Query: 78  RNASATSSVNQFELAKFSAIADTWWDSEGPFKPLHALNPARVSFIRSTLCR--HFRRDPC 137
           R A+ T  VN++     S     W +S       H     +   +R ++ R  HF     
Sbjct: 37  RKANYTDMVNKYYDLATSFYEYGWGES------FHFAQRWKGESLRESIKRHEHFLALQL 96

Query: 138 SIRPFEGLKIIDVGCG-GGILSEPLARMGATVTGIDAVDKNIKIARVHADLDPVTSTIEY 197
            I+P  G K++DVGCG GG L E      + VTG++  +  I   +    L  V  T  +
Sbjct: 97  GIQP--GQKVLDVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNF 156

Query: 198 CCTTAEKLVEQQKEFDAVIASEVIEHVADPAGFCQSLAALTVP 238
                 K+   +  FDAV A E   H  D  G  + +  +  P
Sbjct: 157 VKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVLKP 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O493543.7e-11368.86Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Arabidopsis thalia... [more]
Q2RWE92.4e-6749.80Ubiquinone biosynthesis O-methyltransferase OS=Rhodospirillum rubrum (strain ATC... [more]
B8IUB04.5e-6650.85Ubiquinone biosynthesis O-methyltransferase OS=Methylobacterium nodulans (strain... [more]
A8HVC49.9e-6648.15Ubiquinone biosynthesis O-methyltransferase OS=Azorhizobium caulinodans (strain ... [more]
B0UAV01.4e-6448.35Ubiquinone biosynthesis O-methyltransferase OS=Methylobacterium sp. (strain 4-46... [more]
Match NameE-valueIdentityDescription
XP_023532537.11.15e-238100.00ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita pepo subsp... [more]
KAG7034867.11.14e-23198.49Ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita argyrosper... [more]
KAG6604738.13.25e-23198.19Ubiquinone biosynthesis O-methyltransferase, mitochondrial, partial [Cucurbita a... [more]
XP_022946983.17.66e-23097.89ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita moschata][more]
XP_022970767.16.29e-22997.58ubiquinone biosynthesis O-methyltransferase, mitochondrial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G5C73.71e-23097.89Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucurbita moschata... [more]
A0A6J1I0233.05e-22997.58Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucurbita maxima O... [more]
A0A6J1D8W91.30e-20084.59Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Momordica charanti... [more]
A0A1S3C3341.39e-19484.89Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucumis melo OX=36... [more]
A0A0A0KBI62.69e-19283.99Ubiquinone biosynthesis O-methyltransferase, mitochondrial OS=Cucumis sativus OX... [more]
Match NameE-valueIdentityDescription
AT2G30920.12.6e-11468.86coenzyme Q 3 [more]
AT5G13710.16.5e-0426.99sterol methyltransferase 1 [more]
AT5G13710.26.5e-0426.99sterol methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 90..324
e-value: 1.9E-69
score: 235.3
NoneNo IPR availablePFAMPF13489Methyltransf_23coord: 139..291
e-value: 3.3E-19
score: 69.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..73
NoneNo IPR availablePANTHERPTHR43464METHYLTRANSFERASEcoord: 74..328
NoneNo IPR availablePANTHERPTHR43464:SF19UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIALcoord: 74..328
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 144..244
e-value: 6.02227E-9
score: 51.2767
IPR010233Ubiquinone biosynthesis O-methyltransferaseTIGRFAMTIGR01983TIGR01983coord: 90..320
e-value: 3.7E-89
score: 295.9
IPR010233Ubiquinone biosynthesis O-methyltransferaseHAMAPMF_00472UbiGcoord: 90..326
score: 39.069874
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 94..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g15440.1Cp4.1LG05g15440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
cellular_component GO:0031314 extrinsic component of mitochondrial inner membrane
molecular_function GO:0008425 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity
molecular_function GO:0008689 3-demethylubiquinone-9 3-O-methyltransferase activity
molecular_function GO:0004395 hexaprenyldihydroxybenzoate methyltransferase activity