Cp4.1LG05g13830 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g13830
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPoly(A)-specific ribonuclease
LocationCp4.1LG05: 9705285 .. 9708583 (+)
RNA-Seq ExpressionCp4.1LG05g13830
SyntenyCp4.1LG05g13830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAACCAAGAACAGAGAGATTTTACAGCGATTGAATCAAATGGGTAGAAGAAATCGAGCACGTCCTAGAAGAAGAACACCTTCGATCCAAGAAGTTTGGGCTTCCAATTTCGATCATGAAATCCTCCGCCTCGAACGCTGCCTTCAAGCTAACCATATCCTATCCATCGACACCGAATTTCCTGGGTGCATTAGGAGCACCCCATGGGGCTCGATCGATGAACGCGTCTACGAAGATCTCCGATTCAACGTGAACCAAACCAAGTTAATTCAACTGGGTTTAACAGCTTCTGATGAATCTGGGCAAGTCGCCGGCTCATGGGAGTTCAACTTCTCCGATTTCGACCATCAAATCGACACTCAAAATCCTTCATCGATTTCGTTTCTGGAACAGAACGGTCTCGATTTTGCGAAGCTCAAGAAACAGGGGATCCCCTTAAGCCTATTCACTCTAAAATTCTCGCGTATCATACGAAGAAGCCGTGTTCGTAAATGGGTCACATTCCATGGATTATACGATATTGGGTATCTTGTGAAGGCGATGGGGGTAGCCGAGGAGTTGCCGGAATCAATGGAGGAATTTGCAGAGATGGTGGCGCGTGGAGTTGGGGTTGTGAGGGATTTGAAGGAGGTGGCTCGGTGGTGCGAAGGGCTGGAACAGGGGAAGGTTGGGCTTGAGAGACTTGGGGAATTGCTGGGCGAGAAGAGGTTTGGGATGAAGCACCATGCTGGTTCGGACAGCTTGTTGACTGCTTTTGTGCATGCGAAGATGGCGCGGTGGCTCGGTTTGGAATCAGGGTGTATGACGGCCATTTGTATGGGCTTCAGAACAAGTTCAAGGACATGAACGTGAATCAGAGGATCATGTTTGGGCAGATTTGTTGTTCGTTGTGGGATTATGAATGATTTCTATGAGTTTTTGTTTATCATTATGGAGTAGTTTTAAGTCGATCTCGGTGTAAGTTGAGAAAATCTTCTGAATGAATTTGAAATTTTAGTTGTATTGATTTGATCGTACAACATGTTCAAAGTCTAATTAGATTTGGATGTAACCTTATTCTCGAATTGGTGCCTCGAATAAAACATACATGGAATCAACAGAGAAAGGTCTGATCACAAATGATAAGGAAATCAGTCCAATAGCTCTAATACCACATGATAACGAATCAAACTATAAAACTCTCGCACCAAATAATAAGAAATCACTACAACGGGACAACGGGAGTAAGATTCAAATTAACTTGTTGATCGAATATCTCAAGACAAGAACACGGGTTTGAAATCTGAATCACTTCATAAACAAGACTTATCCTGTCAAACTTTAATGATTATACATGCAATCTAAACTATATAAAATTTTAAAGAAACTTAGTCATTGTTTAAAGAAAAGTACAAAACTCTGTTTAAGTTACTTACAAATACAACATACATACTCTATATAACCTCAAAATAAAACGGTTAAACTCATTACAAGAGTTATAATATTCCTACTGTATAACTAGCGGTAAAGGTAACTAAACTAAATAGGAATAAAACGGTTAAACTCATTACAAGAGTTATAATATTCCTACTGTATAACTAGCGGTAAAGGTAACTAAACTAAATAGGAAGTCTTAAAATATATTAATAAAATAAAATAAAATAACTTTATTATTCTTTTAACGTGGCATAAATTGAAATATGTTTTGATAATTTTAACAACATTTTCTTCCCATATTCGAAATATAGTGTATGATTGATGTCTCTTAGTTCATATGTGATACAAATTTTTTCATTTCCAAAAAATTCAACTAAATTCAAATAATAATAATAATAATAATAATCTAACCTGACCTGACCTTTACAGTTTAGATTGGATTGCATTGGTCTAGTTATCAAAGTTTATAAACTCTCTGAAGTTTTCACTATATTTACTTTTAATCATCTTTGTTAATTTTCTATTAGTTAATTAATTTAATGATTAAGTTTTTAACTAATTTTCAACATAAAAAAGTGTAATATTTTGAAAACTAAAGATAGGATAAAAATTAAATTTCAAATTTATGAGTGAAAATAAATATTAATAATAGTTACAAAATCAAATACAATTTGTTGTTAAAGAATAAATAATATATTTTATTGCATTTTAACGGTCCAAATACAGCTATAATTTTGGAGTCTGATTTATTAAATTAATAATTCAGCTCAAGAAAGCATTTATATTCCCATGATAAAAATATTATCATTTTCAAAAATCCCAACAATCCTATATTTTTGGCGCCAAATTCAAATCTACCGCTCTATCTTACCGCTCTCCTATTAGGATAAGGAAATTCGAACATAATTATATTTCTGCTATAAATATTCTCCGTTCGTCGCATATCGAAGAAGTTTGATTCAACGAAGAACACACACACAGCAAATTCAAAAACAAAAATAGAGAGATTTTCTAACGTAATGGATAGAATTGTTCGAGGCCCGAGAGGAGATAACCCTTTGATTAGAGAGGTATGGGCTTACAATCTCGATCATGAAATCATTCGGCTCGACCGTTGTTTGCTATCGTACTCAGTTATGTCGATCGACACTGAATTTCCTGGGTGTATCAAGAGAACTCCTTGGGGCACGATTGATGAAGAACTCTACGCAGACTTTCGATTCAATGTCAACCAAACCAAAGTGATTCAACTGGGTGTGACAGTTTCTGATGAAGTAGGGAACATCGGCGGTACATGGGAGTTTAATTTCTCTGACTTCGATCCTGAAATCGACGCTCATAACCCTGCGTCGATTTGTTTTCTGAAACAGAACGGCTTGGATTTTGGGAAACTGAAAAAAGATGGAATTCAGGTTAGAAAATTCGCAATAAGATTCTTATATACGATACGAAAGCATGCAATATATCGGTGGGTTACATTTCACGGATTATACGATATTGGATATCTGATGTTGGCGATGGGTGTGGTTAAAAGCTTGCCGGAAACATTGGGCGAATTCGAATGGATCGTGGCGAGGAGAGTGGGGACCGTGAGGGATTTAAAACATATGGCGCGATTCTGCGAAGGGCTCGAAGGGGGGAATTTAGGACTCGAGAAATTAGGGCAATTGCTGGACCAAAAACGCTTTGGATTGAAGCATCACGCTGGTTCAGATAGTTTAATGACTGCGCTGCTTCATGAGAAGATGTTGCAACGGTATGATTTCAATGCGGAAATTTGTGATGGGTTTTTGTATGGGTTGTCGAAGAAATTCGAGGAGTTCGTGGGCTTGCAAACACATCGCATATATGTTCAAATTCAGTGTGCGAGAGTAAAAGCTATG

mRNA sequence

CAAACCAAGAACAGAGAGATTTTACAGCGATTGAATCAAATGGGTAGAAGAAATCGAGCACGTCCTAGAAGAAGAACACCTTCGATCCAAGAAGTTTGGGCTTCCAATTTCGATCATGAAATCCTCCGCCTCGAACGCTGCCTTCAAGCTAACCATATCCTATCCATCGACACCGAATTTCCTGGGTGCATTAGGAGCACCCCATGGGGCTCGATCGATGAACGCGTCTACGAAGATCTCCGATTCAACGTGAACCAAACCAAGTTAATTCAACTGGGTTTAACAGCTTCTGATGAATCTGGGCAAGTCGCCGGCTCATGGGAGTTCAACTTCTCCGATTTCGACCATCAAATCGACACTCAAAATCCTTCATCGATTTCGTTTCTGGAACAGAACGGTCTCGATTTTGCGAAGCTCAAGAAACAGGGGATCCCCTTAAGCCTATTCACTCTAAAATTCTCGCGTATCATACGAAGAAGCCGTGTTCGTAAATGGGTCACATTCCATGGATTATACGATATTGGGTATCTTGTGAAGGCGATGGGGGTAGCCGAGGAGTTGCCGGAATCAATGGAGGAATTTGCAGAGATGGTGGCGCGTGGAGTTGGGGTTGTGAGGGATTTGAAGGAGGTGGCTCGGTGGTGCGAAGGGCTGGAACAGGGGAAGGTTGGGCTTGAGAGACTTGGGGAATTGCTGGGCGAGAAGAGGTTTGGGATGAAGCACCATGCTGGTTCGGACAGCTTGTTGACTGCTTTTGTGCATGCGAAGATGGCGCGGTGGCTCGGTTTGGAATCAGGGTGTATGACGGCCATTTGTATGGGCTTCAGAACAAATAACCCTTTGATTAGAGAGGTATGGGCTTACAATCTCGATCATGAAATCATTCGGCTCGACCGTTGTTTGCTATCGTACTCAGTTATGTCGATCGACACTGAATTTCCTGGGTGTATCAAGAGAACTCCTTGGGGCACGATTGATGAAGAACTCTACGCAGACTTTCGATTCAATGTCAACCAAACCAAAGTGATTCAACTGGGTGTGACAGTTTCTGATGAAGTAGGGAACATCGGCGGTACATGGGAGTTTAATTTCTCTGACTTCGATCCTGAAATCGACGCTCATAACCCTGCGTCGATTTGTTTTCTGAAACAGAACGGCTTGGATTTTGGGAAACTGAAAAAAGATGGAATTCAGGTTAGAAAATTCGCAATAAGATTCTTATATACGATACGAAAGCATGCAATATATCGGTGGGTTACATTTCACGGATTATACGATATTGGATATCTGATGTTGGCGATGGGTGTGGTTAAAAGCTTGCCGGAAACATTGGGCGAATTCGAATGGATCGTGGCGAGGAGAGTGGGGACCGTGAGGGATTTAAAACATATGGCGCGATTCTGCGAAGGGCTCGAAGGGGGGAATTTAGGACTCGAGAAATTAGGGCAATTGCTGGACCAAAAACGCTTTGGATTGAAGCATCACGCTGGTTCAGATAGTTTAATGACTGCGCTGCTTCATGAGAAGATGTTGCAACGGTATGATTTCAATGCGGAAATTTGTGATGGGTTTTTGTATGGGTTGTCGAAGAAATTCGAGGAGTTCGTGGGCTTGCAAACACATCGCATATATGTTCAAATTCAGTGTGCGAGAGTAAAAGCTATG

Coding sequence (CDS)

CAAACCAAGAACAGAGAGATTTTACAGCGATTGAATCAAATGGGTAGAAGAAATCGAGCACGTCCTAGAAGAAGAACACCTTCGATCCAAGAAGTTTGGGCTTCCAATTTCGATCATGAAATCCTCCGCCTCGAACGCTGCCTTCAAGCTAACCATATCCTATCCATCGACACCGAATTTCCTGGGTGCATTAGGAGCACCCCATGGGGCTCGATCGATGAACGCGTCTACGAAGATCTCCGATTCAACGTGAACCAAACCAAGTTAATTCAACTGGGTTTAACAGCTTCTGATGAATCTGGGCAAGTCGCCGGCTCATGGGAGTTCAACTTCTCCGATTTCGACCATCAAATCGACACTCAAAATCCTTCATCGATTTCGTTTCTGGAACAGAACGGTCTCGATTTTGCGAAGCTCAAGAAACAGGGGATCCCCTTAAGCCTATTCACTCTAAAATTCTCGCGTATCATACGAAGAAGCCGTGTTCGTAAATGGGTCACATTCCATGGATTATACGATATTGGGTATCTTGTGAAGGCGATGGGGGTAGCCGAGGAGTTGCCGGAATCAATGGAGGAATTTGCAGAGATGGTGGCGCGTGGAGTTGGGGTTGTGAGGGATTTGAAGGAGGTGGCTCGGTGGTGCGAAGGGCTGGAACAGGGGAAGGTTGGGCTTGAGAGACTTGGGGAATTGCTGGGCGAGAAGAGGTTTGGGATGAAGCACCATGCTGGTTCGGACAGCTTGTTGACTGCTTTTGTGCATGCGAAGATGGCGCGGTGGCTCGGTTTGGAATCAGGGTGTATGACGGCCATTTGTATGGGCTTCAGAACAAATAACCCTTTGATTAGAGAGGTATGGGCTTACAATCTCGATCATGAAATCATTCGGCTCGACCGTTGTTTGCTATCGTACTCAGTTATGTCGATCGACACTGAATTTCCTGGGTGTATCAAGAGAACTCCTTGGGGCACGATTGATGAAGAACTCTACGCAGACTTTCGATTCAATGTCAACCAAACCAAAGTGATTCAACTGGGTGTGACAGTTTCTGATGAAGTAGGGAACATCGGCGGTACATGGGAGTTTAATTTCTCTGACTTCGATCCTGAAATCGACGCTCATAACCCTGCGTCGATTTGTTTTCTGAAACAGAACGGCTTGGATTTTGGGAAACTGAAAAAAGATGGAATTCAGGTTAGAAAATTCGCAATAAGATTCTTATATACGATACGAAAGCATGCAATATATCGGTGGGTTACATTTCACGGATTATACGATATTGGATATCTGATGTTGGCGATGGGTGTGGTTAAAAGCTTGCCGGAAACATTGGGCGAATTCGAATGGATCGTGGCGAGGAGAGTGGGGACCGTGAGGGATTTAAAACATATGGCGCGATTCTGCGAAGGGCTCGAAGGGGGGAATTTAGGACTCGAGAAATTAGGGCAATTGCTGGACCAAAAACGCTTTGGATTGAAGCATCACGCTGGTTCAGATAGTTTAATGACTGCGCTGCTTCATGAGAAGATGTTGCAACGGTATGATTTCAATGCGGAAATTTGTGATGGGTTTTTGTATGGGTTGTCGAAGAAATTCGAGGAGTTCGTGGGCTTGCAAACACATCGCATATATGTTCAAATTCAGTGTGCGAGAGTAAAAGCTATG

Protein sequence

QTKNREILQRLNQMGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFTLKFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVGVVRDLKEVARWCEGLEQGKVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWLGLESGCMTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM
Homology
BLAST of Cp4.1LG05g13830 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.7e-49
Identity = 107/255 (41.96%), Postives = 147/255 (57.65%), Query Frame = 0

Query: 278 NNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNV 337
           + PL R VW  N+D E+ R+  CL  + +++ DTE+PG I RT + +  +E Y   + NV
Sbjct: 5   HQPLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNV 64

Query: 338 NQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGI 397
             TK+IQ G T+ +  G IGG WE NFS+F    D  N  SI FL+++GLD  K++ +G+
Sbjct: 65  ENTKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGV 124

Query: 398 QVRKFAI--RFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRV 457
            +  +    + +   R      +VTF G YD  Y +  +   K LPET GEF   V +  
Sbjct: 125 DMFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGK-LPETHGEFATEVVKVF 184

Query: 458 GTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRYD 517
           G V D K MA FCEGL G +LGL KL QLL   R G  HHAGSDSLMTAL+  K+   Y+
Sbjct: 185 GQVYDTKVMAGFCEGL-GEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE 244

Query: 518 FNAEICDGFLYGLSK 531
            ++    G +YG+ K
Sbjct: 245 -DSRFARGLIYGIGK 256

BLAST of Cp4.1LG05g13830 vs. ExPASy Swiss-Prot
Match: Q9SFX6 (Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CAF1-3 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 3.6e-44
Identity = 100/229 (43.67%), Postives = 140/229 (61.14%), Query Frame = 0

Query: 31  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 90
           EVW  N + E+  +  CL+    ++IDTEFPGC++ TP  + +E  Y D++FNV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 91  QLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 150
           QLG T  D  G  A +WE N SDFD     +N  SI+FL+ NGL+  K++++GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 151 LKFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 210
             FS+I+     +  WV F G YD  YLVK +   + LPE+ EEF E V + +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 211 KEVARWCEGLEQGKVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 258
           K++A  C GL   + GL+R+ ++L  KR G  HHAGSDS LTA V  K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

BLAST of Cp4.1LG05g13830 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 4.7e-44
Identity = 97/229 (42.36%), Postives = 144/229 (62.88%), Query Frame = 0

Query: 31  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 90
           EVW  N   E+  +  CL+  + ++IDTEFPGC++ TP  + DE  Y D++FNV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 91  QLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 150
           QLGLT   +   +  +WE N SDF+     +N  SI+FL+ NGLD  K++++GI +  F 
Sbjct: 64  QLGLTLFGKG--ITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 151 LKFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 210
           ++FS+I+     + +WVTF G YD  YL+K +   + LPE+ +EF E V + +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 211 KEVARWCEGLEQGKVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 258
           K++A  C GL   + GL+R+ ++L  +R G  HHAGSDS LTA V  K+
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

BLAST of Cp4.1LG05g13830 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 6.2e-44
Identity = 98/219 (44.75%), Postives = 134/219 (61.19%), Query Frame = 0

Query: 297 LDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVIQLGVTVSDEVGNI 356
           ++ CL SY  ++IDTEFP  ++ T     DEE Y D  F+V++ K+IQLG+T+ D  G I
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 357 GGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIQVRKFAIRFLYTIRK-HAI 416
           GGTWE NFSDF  + DA N  SI FL++NGLD  K++++GI++  F     + ++K    
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 417 YRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGTVRDLKHMARFCEGLEGGN 476
             WVTFHG YDI YL+      ++LP T   F   VAR +G+V DLK MA  CEGL    
Sbjct: 124 ITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSR 183

Query: 477 LGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRY 515
           LGLE L       R G  HHAGS++ +TA++  K+L  +
Sbjct: 184 LGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

BLAST of Cp4.1LG05g13830 vs. ExPASy Swiss-Prot
Match: Q9C6M9 (Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=CAF1-4 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 9.8e-42
Identity = 96/229 (41.92%), Postives = 131/229 (57.21%), Query Frame = 0

Query: 284 EVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 343
           EVW +N + E+  +  CL  +S ++IDTEFPGC+K TP    +E  Y D +FNV+ T +I
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 344 QLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIQVRKFA 403
           QLG T+ D  G I  TWE N SDF+      N  SI FLK NGL+  K+ ++GI + +F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 404 IRFLYTIR-KHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 463
             F   ++ K     WV F G YD  YL+  +   K LPET  EF   V + +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 464 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKM 511
           K +A  C GL     GL+++  +L  KR G  HHAGSDS +TA +  K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

BLAST of Cp4.1LG05g13830 vs. NCBI nr
Match: XP_023533172.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 580 bits (1496), Expect = 1.42e-203
Identity = 281/288 (97.57%), Postives = 282/288 (97.92%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM
Sbjct: 241 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 288

BLAST of Cp4.1LG05g13830 vs. NCBI nr
Match: KAG7027009.1 (putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 571 bits (1472), Expect = 6.28e-200
Identity = 274/288 (95.14%), Postives = 281/288 (97.57%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLIREVWAYNLDHEIIRLDRCL+SYSVMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE+GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+RKHAIYRW+TFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQRYDF+AEICDGFLYGLSKKFEE VGLQTHRIYVQIQCARVKAM
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAM 288

BLAST of Cp4.1LG05g13830 vs. NCBI nr
Match: KAG6604930.1 (putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 570 bits (1470), Expect = 1.26e-199
Identity = 273/288 (94.79%), Postives = 281/288 (97.57%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLIREVWAYNLDHEIIRLDRCL+SYSVMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE+GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRF+YT+RKHAIYRW+TFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFIYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQRYDF+AEICDGFLYGLSKKFEE VGLQTHRIYVQIQCARVKAM
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAM 288

BLAST of Cp4.1LG05g13830 vs. NCBI nr
Match: XP_022947868.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 565 bits (1457), Expect = 1.19e-197
Identity = 272/288 (94.44%), Postives = 278/288 (96.53%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLIREVWAYNLDHEIIRLDRCLLSYS+MSIDTEFPGCIKRTPWGT DE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+ KHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           +WIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 KWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQRYDF+AEICDGFLYGLSKKFEE VGLQTHRIYVQIQCARVKAM
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAM 288

BLAST of Cp4.1LG05g13830 vs. NCBI nr
Match: XP_022970734.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima] >XP_022970738.1 putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima])

HSP 1 Score: 548 bits (1412), Expect = 7.00e-191
Identity = 262/288 (90.97%), Postives = 274/288 (95.14%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLI EVWAYNLD EI+RLD CLL Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+RKHAI++W+TFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQ YDFNAEICDGFLYGLSKKFEEFVG+Q+HRIYVQI+CA VKAM
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAM 288

BLAST of Cp4.1LG05g13830 vs. ExPASy TrEMBL
Match: A0A6J1G7T4 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3 SV=1)

HSP 1 Score: 565 bits (1457), Expect = 5.77e-198
Identity = 272/288 (94.44%), Postives = 278/288 (96.53%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLIREVWAYNLDHEIIRLDRCLLSYS+MSIDTEFPGCIKRTPWGT DE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+ KHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           +WIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 KWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQRYDF+AEICDGFLYGLSKKFEE VGLQTHRIYVQIQCARVKAM
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAM 288

BLAST of Cp4.1LG05g13830 vs. ExPASy TrEMBL
Match: A0A6J1I6H7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 3.39e-191
Identity = 262/288 (90.97%), Postives = 274/288 (95.14%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLI EVWAYNLD EI+RLD CLL Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+RKHAI++W+TFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQ YDFNAEICDGFLYGLSKKFEEFVG+Q+HRIYVQI+CA VKAM
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAM 288

BLAST of Cp4.1LG05g13830 vs. ExPASy TrEMBL
Match: A0A6J1I3Q4 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469634 PE=3 SV=1)

HSP 1 Score: 546 bits (1408), Expect = 5.30e-191
Identity = 261/288 (90.62%), Postives = 273/288 (94.79%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLI EVWAYNLD EI+RLD CLL Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+RKHAI++W+TFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMT LLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTVLLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQ YDFNAEICDGFLYGLSKKFEEFVG+Q+HRIYVQI+CA VKAM
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAM 288

BLAST of Cp4.1LG05g13830 vs. ExPASy TrEMBL
Match: A0A6J1I4S7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469631 PE=3 SV=1)

HSP 1 Score: 546 bits (1407), Expect = 1.95e-190
Identity = 261/288 (90.62%), Postives = 274/288 (95.14%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLI EVWAYNLD EI+RLD CLL Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQLGVTVSDE G+IGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGDIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+RKHAI++W+TFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQ YDFNAEICDGFLYGLSKKFEEFVG+Q+HRIYVQI+CA VKAM
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAM 288

BLAST of Cp4.1LG05g13830 vs. ExPASy TrEMBL
Match: A0A6J1I1E6 (putative CCR4-associated factor 1 homolog 8 OS=Cucurbita maxima OX=3661 GN=LOC111469635 PE=4 SV=1)

HSP 1 Score: 545 bits (1403), Expect = 7.88e-190
Identity = 261/288 (90.62%), Postives = 273/288 (94.79%), Query Frame = 0

Query: 268 MTAICMGFRTNNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 327
           M  I  G R +NPLI EVWAYNLD EI+RLD CLL Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 328 ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 387
           ELYADFRFNVNQTKVIQL VTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLCVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 388 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 447
           DFGKLKKDGI+VRKFAIRFLYT+RKHAI++W+TFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 448 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 507
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 508 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAM 555
           EKMLQ YDFNAEICDGFLYGLSKKFEEFVG+Q+HRIYVQI+CA VKAM
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAM 288

BLAST of Cp4.1LG05g13830 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 2.7e-50
Identity = 107/255 (41.96%), Postives = 147/255 (57.65%), Query Frame = 0

Query: 278 NNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNV 337
           + PL R VW  N+D E+ R+  CL  + +++ DTE+PG I RT + +  +E Y   + NV
Sbjct: 5   HQPLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNV 64

Query: 338 NQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGI 397
             TK+IQ G T+ +  G IGG WE NFS+F    D  N  SI FL+++GLD  K++ +G+
Sbjct: 65  ENTKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGV 124

Query: 398 QVRKFAI--RFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRV 457
            +  +    + +   R      +VTF G YD  Y +  +   K LPET GEF   V +  
Sbjct: 125 DMFGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGK-LPETHGEFATEVVKVF 184

Query: 458 GTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRYD 517
           G V D K MA FCEGL G +LGL KL QLL   R G  HHAGSDSLMTAL+  K+   Y+
Sbjct: 185 GQVYDTKVMAGFCEGL-GEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE 244

Query: 518 FNAEICDGFLYGLSK 531
            ++    G +YG+ K
Sbjct: 245 -DSRFARGLIYGIGK 256

BLAST of Cp4.1LG05g13830 vs. TAIR 10
Match: AT1G27820.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 2.6e-45
Identity = 100/229 (43.67%), Postives = 140/229 (61.14%), Query Frame = 0

Query: 31  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 90
           EVW  N + E+  +  CL+    ++IDTEFPGC++ TP  + +E  Y D++FNV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 91  QLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 150
           QLG T  D  G  A +WE N SDFD     +N  SI+FL+ NGL+  K++++GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 151 LKFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 210
             FS+I+     +  WV F G YD  YLVK +   + LPE+ EEF E V + +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 211 KEVARWCEGLEQGKVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 258
           K++A  C GL   + GL+R+ ++L  KR G  HHAGSDS LTA V  K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

BLAST of Cp4.1LG05g13830 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 3.4e-45
Identity = 97/229 (42.36%), Postives = 144/229 (62.88%), Query Frame = 0

Query: 31  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 90
           EVW  N   E+  +  CL+  + ++IDTEFPGC++ TP  + DE  Y D++FNV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 91  QLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 150
           QLGLT   +   +  +WE N SDF+     +N  SI+FL+ NGLD  K++++GI +  F 
Sbjct: 64  QLGLTLFGKG--ITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 151 LKFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 210
           ++FS+I+     + +WVTF G YD  YL+K +   + LPE+ +EF E V + +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 211 KEVARWCEGLEQGKVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 258
           K++A  C GL   + GL+R+ ++L  +R G  HHAGSDS LTA V  K+
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

BLAST of Cp4.1LG05g13830 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 4.4e-45
Identity = 98/219 (44.75%), Postives = 134/219 (61.19%), Query Frame = 0

Query: 297 LDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVIQLGVTVSDEVGNI 356
           ++ CL SY  ++IDTEFP  ++ T     DEE Y D  F+V++ K+IQLG+T+ D  G I
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 357 GGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIQVRKFAIRFLYTIRK-HAI 416
           GGTWE NFSDF  + DA N  SI FL++NGLD  K++++GI++  F     + ++K    
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 417 YRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGTVRDLKHMARFCEGLEGGN 476
             WVTFHG YDI YL+      ++LP T   F   VAR +G+V DLK MA  CEGL    
Sbjct: 124 ITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSR 183

Query: 477 LGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRY 515
           LGLE L       R G  HHAGS++ +TA++  K+L  +
Sbjct: 184 LGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

BLAST of Cp4.1LG05g13830 vs. TAIR 10
Match: AT1G27890.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 7.0e-43
Identity = 96/229 (41.92%), Postives = 131/229 (57.21%), Query Frame = 0

Query: 284 EVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 343
           EVW +N + E+  +  CL  +S ++IDTEFPGC+K TP    +E  Y D +FNV+ T +I
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 344 QLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIQVRKFA 403
           QLG T+ D  G I  TWE N SDF+      N  SI FLK NGL+  K+ ++GI + +F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 404 IRFLYTIR-KHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 463
             F   ++ K     WV F G YD  YL+  +   K LPET  EF   V + +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 464 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKM 511
           K +A  C GL     GL+++  +L  KR G  HHAGSDS +TA +  K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SHJ03.7e-4941.96Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SFX63.6e-4443.67Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
O647734.7e-4442.36Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LXM46.2e-4444.75Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9C6M99.8e-4241.92Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
XP_023533172.11.42e-20397.57putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo][more]
KAG7027009.16.28e-20095.14putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp.... [more]
KAG6604930.11.26e-19994.79putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022947868.11.19e-19794.44putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata][more]
XP_022970734.17.00e-19190.97putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima] >XP_022970738.1 p... [more]
Match NameE-valueIdentityDescription
A0A6J1G7T45.77e-19894.44Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3... [more]
A0A6J1I6H73.39e-19190.97Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 S... [more]
A0A6J1I3Q45.30e-19190.63Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469634 PE=3 S... [more]
A0A6J1I4S71.95e-19090.63Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469631 PE=3 S... [more]
A0A6J1I1E67.88e-19090.63putative CCR4-associated factor 1 homolog 8 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G06450.12.7e-5041.96Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G27820.12.6e-4543.67Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G61470.13.4e-4542.36Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G44240.14.4e-4544.75Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G27890.17.0e-4341.92Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 17..271
e-value: 1.3E-64
score: 219.9
coord: 272..532
e-value: 1.6E-62
score: 213.0
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 288..398
e-value: 1.3E-5
score: 24.6
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 32..145
e-value: 2.6E-10
score: 40.1
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 280..547
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 27..268
NoneNo IPR availablePANTHERPTHR10797:SF57CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATEDcoord: 27..268
NoneNo IPR availablePANTHERPTHR10797:SF57CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATEDcoord: 280..547
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 282..518
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 23..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g13830.1Cp4.1LG05g13830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006402 mRNA catabolic process
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030014 CCR4-NOT complex
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity