Cp4.1LG05g07750 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g07750
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNon-specific serine/threonine protein kinase
LocationCp4.1LG05: 4821817 .. 4825567 (-)
RNA-Seq ExpressionCp4.1LG05g07750
SyntenyCp4.1LG05g07750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGATGTTTATATGGCCGACCCTGTTTTTCCCTTGATCATCATGCGTCAGAATTTCGTTCATCCAATTTATTCTTACGAATTTCAATCCGCTATGGAATTGTTCATGGGTCGCCAATTTCACTTTCAATCTGCATTTGATGTGGCTTCCGAGGCCTTTTCTGAATACCTTTCTCGTTTGATCGTCGTTATCTGACCGTTTGCTCTTTGAGCAGAGGATTTTTTTTTTTTTTTATCTCTTTTTTCTTTTTTCGCGATTTTTGTTTCTATTTCTTCGTCTTTCTGCGTTGCGATGAGTTGTGGTTCGGATTTCATGTCTGGGAATTCTGAGGCTGATGATCTTAATATTTTAGAGAAGGATCCAACTGGACGATATATACGGGTATTTCTTTTCTATTTCTTCTTCGATTTTGGATGTTTTGATTCGTATCTACCGCTTCTTGTTCTTCAGCTAATTACCCTAGCGATTGATTTACATTTTATCCAGGTGTTTAATTACTCTATTTTGAAATCTGATTTGAGTGATCTTAATTTTGGGATTCACTGCTGATTTTGTGAGGGGAGGAGGAGAAATTTTGGGCATAACCACCAAACTTCTTATTATTTTGGTTTAACGATCGACTACTAATGTTTTAAGTTTTTTCTTTCCGTTTTGCTTCATAGTTGAGATGTTCTTATTCTATTTGGCGTATGATTTAATCCTCGTTTTTCATATATATATTTTTCAGTACGATGAGATCTTGGGTAAAGGGGCATTCAAGACAGTGTATGCTTCTGCTGTCTATCAATCTCAGTTCTTTTATTACTTTTTTTCTTTTTCTTTTCTTTTTTTTTNTTAGGTTGAATATTGTTGGATCAAGAAAGGGTATATTATTTGACTTGAGATCTGTTTTTGTTGTCATCAGATTCAAAGCTTTCGATAAAGCTGGTGGAATCGAAGTTGCTTGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCTGAACAACTTCAAAGATTGTATTCTGAGGTTCATTTGCTGAAGTCACTGAAACATGAGAATATTATCAAGTTCTATAGTTACTGGGTTGATGATAACACTAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCAGTAAGAACCACCAAAGAGTCTTCGTTTTGTTTCGGTTATTCGTTTCTTATTATCATTTTACTGCATTCAATGAGTTATTTGTTCACTTATGGTATACTTCGGAATTCAGATATCGAAAGAAGCACAGGAAAGTGGACTTGAAGGCTTTCAAGAACTGGGCAAGGAAGATACTGCGGGGTTTAACGTATTTGCACGGTCACGATCCTCCAATTATTCACAGGGATTTGAAATGTGATAATATATTTGTCAATGGAAATACTGGAGAAGTTAAAATTGGAGATCTCGGATTGGCAATCGTCTTGCAGCAGCCGACTGCCCGAAGTGTAATTGGTAATTTTCATTTTCTTGCTCGATTTCTAGATTGAAGCAGAACGAACTTCAACTAATATTTGATGATTATTATCTAGGCACTCCAGAGTTCATGGCTCCAGAGCTCTATGATGAGGACTACAATGAACTGGTTGATATCTATTCTTTTGGCATGTGCATGTTGGAAATTGTTACTTGTGAATACCCATATAATGAGTGCAAAAATTCTGCCCAAATATTCAAGAAGGTTACTTCTGTAAGTAGAACATGATTTACCTCTTATCAGATTATGTCCACTAAATGTTTCCATCAAATTTGAAGTTATGGAATCATTTTCTTGTTTCTTCCAGGGTGTAATGCCTGCTTCCCTTGAAAAAGTGTTGGATCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCATTCCTTGCAACTGAAAATCGCAAGGATCATAGTTCTGAACGTTCAATGTTATTGAATGCTCAGTTCGAATCAGTAAATCCTCCTCTGCTGGATACTAATCCAATGGACATGGATCACAATTGCAGCAAACTCTCAGGCTCAGTGGCCTCATCTATCGAAAGCAATAATGAAATTTCTCACTTTTCAACTCAGGAACTCAAGACGCTCACTGAGAACAATGAATTGACACTGAAAGGGGACATGACTGATCATAATTCAATTTCGCTCCATTTGCGCATTGCCGAACTATCTGGTAACTTTTTCGGTCAACTCATCTGTAATCTACTAAAAGTTCTTTGATACACATGATGGTAACTTGAACATTTATTTTAGGTCAATCTAAGAATATCCATTTTGCATTCTTTCTCGATTCCGATACTGCGTTGGCTATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCTACCATCATAGCACAACTGATAGATGAATTAATTGCCAAGTTTGTTCCCAGTTGGCAACAATGTACAAATTTTTGTGACGGACTAATTACTGAACATAGTTCTTCTGAAACTGAAGAGGACAAGGCCTTCAGTCCTCCTCTCTTCTCTGAGATGGTACTCTCTTCTCCCTTGGTGGGTGCCGGTAGGAAAACTCTGACAGAATTGGCGGAGGTTGAACATCAAGAAAACCAGCAGACAATCATCTCATGTGCATCTCTGGACTACAACCACTCAACCATCTCAGATTACAGCCTGGGAAAAGGAGGATCAGAGTGTGGTGACTTCAGCCACCCAGAATGCGACAAAGCTTTTATAAGCAGCGGCAGGGGAACCAGCGATTTAGATGCTGTTGGATCTGTTTCGACTATGTTCGATTTTGCCAAACCGTCCCTAATCTCCTCCTGCTCAGGAATGTCGAAAGAATTGAGCTTGTCTAACTTCTCCAATTTTTCTGTGGAAGAAAGAGACCAGCATGATGAACTGAAGATGGAGATTGATGCCATTGACATGCAGTACCACCAGTGCCTTTGTGAACTGTCGAGAATGAGAGAGGAAGCTATTGAAAGTGCCAAGAAGAGGTGGATGTCAAAGAAGAAAGGTGCTGCTATTTGAATGAAGCCCTCTGGATTTACAAGTTTGTTTTCTTCATCTTCTTCTTCTTCTTCATCTTCTTCTTCTTCTTCATTTTTTGGCAACTCTGGCAAGATGGATGATGGTCATGTTCTTATTTTACTACTGAGCTTATATTTTAGCAGTCTATCCATCCATCCAACCAAGTCCTAACTCCCAACATCTTCTCACTCTGGGCTCAGCCATGTTTAACTTATATATATATATATATATATATATATATATATATATATATATATATATACCAATTCATTTCATAGTTTAGACACATTCATTCATTGTCACATTATTCTTCTCATAGTGCAAACTATTTTCCATGTATTATTCTTACTCATTCCCATGAGTTCTTTCTGCAATTTCTTCCATATGAAAAAACTATTCTTATAATTTTGCTTTCAGCTGTTTCTTTTTTCCAACATTCAGTTATATCCTTGTAACTTACCTTGCAAGTCAAGAATTCAATTTATGTATTAATATTATCATTTTGTTCCAGTGTTCAATGGGGTTTTTCTTTTACTCAACGTGTAGCATGCTCTTGAAAACTAGCTTGTTCGGCTCGCCTCGCCTCGGTCTTTTTAGTCACCAGTGACAAATGTTCCAGTGTTCAATGGGATCTTACAATTGTTTCATGTAATCACCACCAAGGTCCCTAATTTTGACTCGGGAACTATATACAATGATATGCATATTTGGCCA

mRNA sequence

ATGGTGATTTGTGGTTCGGATTTCATGTCTGGGAATTCTGAGGCTGATGATCTTAATATTTTAGAGAAGGATCCAACTGGACGATATATACGGTACGATGAGATCTTGGGTAAAGGGGCATTCAAGACAGTATTCAAAGCTTTCGATAAAGCTGGTGGAATCGAAGTTGCTTGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCTGAACAACTTCAAAGATTGTATTCTGAGGTTCATTTGCTGAAGTCACTGAAACATGAGAATATTATCAAGTTCTATAGTTACTGGGTTGATGATAACACTAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCACAGCCGACTGCCCGAAGGTGTAATGCCTGCTTCCCTTGAAAAAGTGTTGGATCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCATTCCTTGCAACTGAAAATCGCAAGGATCATAGTTCTGAACGTTCAATGTTATTGAATGCTCAGTTCGAATCAGTAAATCCTCCTCTGCTGGATACTAATCCAATGGACATGGATCACAATTGCAGCAAACTCTCAGGCTCAGTGGCCTCATCTATCGAAAGCAATAATGAAATTTCTCACTTTTCAACTCAGGAACTCAAGACGCTCACTGAGAACAATGAATTGACACTGAAAGGGGACATGACTGATCATAATTCAATTTCGCTCCATTTGCGCATTGCCGAACTATCTGGTCAATCTAAGAATATCCATTTTGCATTCTTTCTCGATTCCGATACTGCGTTGGCTATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCTACCATCATAGCACAACTGATAGATGAATTAATTGCCAAGTTTGTTCCCAGTTGGCAACAATGTACAAATTTTTGTGACGGACTAATTACTGAACATAGTTCTTCTGAAACTGAAGAGGACAAGGCCTTCAGTCCTCCTCTCTTCTCTGAGATGGTACTCTCTTCTCCCTTGGTGGGTGCCGGTAGGAAAACTCTGACAGAATTGGCGGAGGTTGAACATCAAGAAAACCAGCAGACAATCATCTCATGTGCATCTCTGGACTACAACCACTCAACCATCTCAGATTACAGCCTGGGAAAAGGAGGATCAGAGTGTGGTGACTTCAGCCACCCAGAATGCGACAAAGCTTTTATAAGCAGCGGCAGGGGAACCAGCGATTTAGATGCTGTTGGATCTGTTTCGACTATGTTCGATTTTGCCAAACCGTCCCTAATCTCCTCCTGCTCAGGAATGTCGAAAGAATTGAGCTTGTCTAACTTCTCCAATTTTTCTGTGGAAGAAAGAGACCAGCATGATGAACTGAAGATGGAGATTGATGCCATTGACATGCAGTACCACCAGTGCCTTTGTGAACTGTCGAGAATGAGAGAGGAAGCTATTGAAAGTGCCAAGAAGAGGTGGATGTCAAAGAAGAAAGGTGCTGCTATTTGAATGAAGCCCTCTGGATTTACAATGTTCAATGGGGTTTTTCTTTTACTCAACGTGTAGCATGCTCTTGAAAACTAGCTTGTTCGGCTCGCCTCGCCTCGGTCTTTTTAGTCACCAGTGACAAATGTTCCAGTGTTCAATGGGATCTTACAATTGTTTCATGTAATCACCACCAAGGTCCCTAATTTTGACTCGGGAACTATATACAATGATATGCATATTTGGCCA

Coding sequence (CDS)

ATGGTGATTTGTGGTTCGGATTTCATGTCTGGGAATTCTGAGGCTGATGATCTTAATATTTTAGAGAAGGATCCAACTGGACGATATATACGGTACGATGAGATCTTGGGTAAAGGGGCATTCAAGACAGTATTCAAAGCTTTCGATAAAGCTGGTGGAATCGAAGTTGCTTGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCTGAACAACTTCAAAGATTGTATTCTGAGGTTCATTTGCTGAAGTCACTGAAACATGAGAATATTATCAAGTTCTATAGTTACTGGGTTGATGATAACACTAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCACAGCCGACTGCCCGAAGGTGTAATGCCTGCTTCCCTTGAAAAAGTGTTGGATCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCATTCCTTGCAACTGAAAATCGCAAGGATCATAGTTCTGAACGTTCAATGTTATTGAATGCTCAGTTCGAATCAGTAAATCCTCCTCTGCTGGATACTAATCCAATGGACATGGATCACAATTGCAGCAAACTCTCAGGCTCAGTGGCCTCATCTATCGAAAGCAATAATGAAATTTCTCACTTTTCAACTCAGGAACTCAAGACGCTCACTGAGAACAATGAATTGACACTGAAAGGGGACATGACTGATCATAATTCAATTTCGCTCCATTTGCGCATTGCCGAACTATCTGGTCAATCTAAGAATATCCATTTTGCATTCTTTCTCGATTCCGATACTGCGTTGGCTATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCTACCATCATAGCACAACTGATAGATGAATTAATTGCCAAGTTTGTTCCCAGTTGGCAACAATGTACAAATTTTTGTGACGGACTAATTACTGAACATAGTTCTTCTGAAACTGAAGAGGACAAGGCCTTCAGTCCTCCTCTCTTCTCTGAGATGGTACTCTCTTCTCCCTTGGTGGGTGCCGGTAGGAAAACTCTGACAGAATTGGCGGAGGTTGAACATCAAGAAAACCAGCAGACAATCATCTCATGTGCATCTCTGGACTACAACCACTCAACCATCTCAGATTACAGCCTGGGAAAAGGAGGATCAGAGTGTGGTGACTTCAGCCACCCAGAATGCGACAAAGCTTTTATAAGCAGCGGCAGGGGAACCAGCGATTTAGATGCTGTTGGATCTGTTTCGACTATGTTCGATTTTGCCAAACCGTCCCTAATCTCCTCCTGCTCAGGAATGTCGAAAGAATTGAGCTTGTCTAACTTCTCCAATTTTTCTGTGGAAGAAAGAGACCAGCATGATGAACTGAAGATGGAGATTGATGCCATTGACATGCAGTACCACCAGTGCCTTTGTGAACTGTCGAGAATGAGAGAGGAAGCTATTGAAAGTGCCAAGAAGAGGTGGATGTCAAAGAAGAAAGGTGCTGCTATTTGA

Protein sequence

MVICGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSRLPEGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQHDELKMEIDAIDMQYHQCLCELSRMREEAIESAKKRWMSKKKGAAI
Homology
[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy Swiss-Prot
Match: Q944Q0 (Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.5e-76
Identity = 216/628 (34.39%), Postives = 305/628 (48.57%), Query Frame = 0 

Query: 5   GSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIE 64
           GS F+   S  ++ +  EKDP+GRYIRYD++LG+GAFKTV+KAFD+  GIEVAW+ V IE
Sbjct: 4   GSGFLGQISSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIE 63

Query: 65  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR----- 124
           DV+Q P QL+RLYSEVHLLK+LKHENIIK +  WVD+  KTINMITELFTSGSLR     
Sbjct: 64  DVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKK 123

Query: 125 -----------------------HSRLP-------------------------------- 184
                                  HS+ P                                
Sbjct: 124 HRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVL 183

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 184 QQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKV 243

Query: 245 -EGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLLN 304
              + P SL KV DPQV+QFIEKCL+PAS R  A EL KDPFLA +  KD +   S   +
Sbjct: 244 TSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSALLASSSTS 303

Query: 305 AQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLKG 364
           +++  V PP L+  PMD+DHN +K       S+ SN +     T EL+ + EN E  L+G
Sbjct: 304 SKY--VRPPQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFRLRG 363

Query: 365 DMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLI 424
           + +D  + S+ LRIA+ SG+ + +HFAF+L+SDTA AIA EMVE+L L++++  +IA +I
Sbjct: 364 ERSDDVTASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMI 423

Query: 425 DELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTE 484
           D+ I +              L+++ +SS   ++   SP L  E                 
Sbjct: 424 DDFIMQ--------------LLSDRTSSHHNQN---SPRLTHE----------------- 483

Query: 485 LAEVEHQ-ENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 511
               +H+  NQQT+ S        S  SD S         D+  P       S+  G + 
Sbjct: 484 ----DHEAANQQTVNSKDEEAAGQSMKSDIS--------ADYYFP------YSANDGNAA 543

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy Swiss-Prot
Match: Q6EU49 (Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK4 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.3e-74
Identity = 217/624 (34.78%), Postives = 298/624 (47.76%), Query Frame = 0 

Query: 14  EADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIEDVLQSPEQL 73
           + DD   +E DPT RYIRY+E+LG+GA KTV+KAFD+  GIEVAWSQV+I++V+QSP+ L
Sbjct: 9   KVDDAECVEVDPTRRYIRYNEVLGRGAMKTVYKAFDEVEGIEVAWSQVEIDEVMQSPDNL 68

Query: 74  QRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR----------- 133
           +RLYSEVHLLKSLKHEN++KFY+YWVDD  KTIN+ITELFTSGSLR  R           
Sbjct: 69  ERLYSEVHLLKSLKHENVMKFYNYWVDDQKKTINVITELFTSGSLRQYRQKHPRVDLKAI 128

Query: 134 ------------------------------------------------------------ 193
                                                                       
Sbjct: 129 KNWARQVLRGLDYLHTHQPPIIHRDLKCDNIFVNGNHGEVKIGDLGLATVMLTPRAKSVI 188

Query: 194 --------------------------------------------------LPEGVMPASL 253
                                                             + +GV PA+L
Sbjct: 189 GTPEFMAPELYDENYDELVDIYSFGMCMLEMFTLEYPYSECTNAAQIFKKVSKGVKPAAL 248

Query: 254 EKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLLNAQFESVNPP 313
            K+ + Q KQFI+KCLVPAS RL A ELL+DPFL ++N        S+L+  +F S  P 
Sbjct: 249 AKITNIQAKQFIDKCLVPASERLSAKELLQDPFLCSDN-------SSVLVGTKFPSSLPK 308

Query: 314 LLDTN----PMDMDHNCSKLSGSVASSIESNNEIS--HFSTQELKTLTENNELTLKGDMT 373
            +D +     MD+D N      S+ +S    N++   H S  E     +N EL L G+  
Sbjct: 309 SVDVSLEALHMDVDTN-----ESMCTSTCKRNDLGGPHRSVLEFTRTNKNTELKLTGEKL 368

Query: 374 DHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLIDEL 433
           D NS+SL LRIA+L G ++NIHF F+LDSDTA+++A EMVEQLEL++ D T IA  ID L
Sbjct: 369 DDNSVSLVLRIADLCGHARNIHFLFYLDSDTAMSVAAEMVEQLELADCDVTFIADFIDLL 428

Query: 434 IAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTELAE 493
           I   VP  QQ  N  D +++  S S+  E +           L+   V       ++ A 
Sbjct: 429 IVNLVPG-QQLMN--DAVMSTSSESKMGESEHVITSQQHPSELTHDYVLVEGMMHSKEAN 488

Query: 494 VEHQENQQTIISCASL-DYNHSTISDYSLGKGGS--ECGDFSHPECDKAFISSGRGTSDL 508
               +   ++++  +L   N S  SD S+   GS     ++   E       + +GT   
Sbjct: 489 ASPSDYIDSLLNATNLGGPNSSEGSDISVQLDGSSKSLSEYGVDEYRTLECGAYKGT--- 548

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy Swiss-Prot
Match: Q8RXE5 (Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 GN=WNK10 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.4e-65
Identity = 196/610 (32.13%), Postives = 272/610 (44.59%), Query Frame = 0 

Query: 16  DDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIEDVLQSPEQLQR 75
           ++ + ++KDPTGRYIRY+++LG+GAFKTV+KAFD+  GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 76  LYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR---------------- 135
           LYSEVHLL SLKH+NIIK +  WVDD+ K+INMITELFTSGSL                 
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 136 ------------HSRLP------------------------------------------- 195
                       HS+ P                                           
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 196 --------------------------------------------------EGVMPASLEK 255
                                                              G+ P SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 256 VLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLLNAQFESVNPPLL 315
           V DPQVKQFIEKCL+PA  R  A ELLKD  LA +  KD +   S   N  F+   PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTLTASS--NTTFKPAMPPQC 301

Query: 316 DTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLKGDMTDHNSISLH 375
           +  PMD+++     S S+ SS +S+ E +   T E++ + E+ E  L G+  D  + S+ 
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 376 LRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLIDELIAKFVPSW 435
           LRIA  SGQ++ + F F L +DTA A+  EMVE+L+LS+ + T+IA++IDELI K   + 
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMKLKAN- 421

Query: 436 QQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTELAEVEHQENQQ 495
                    L   +S  ++++++                  AG    +E+          
Sbjct: 422 -------RSLPNANSVYQSKDEE------------------AGESMKSEI---------- 481

Query: 496 TIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSDLDAVGSVSTMFD 505
                 S DY H                           +SS  G+     +G      +
Sbjct: 482 ------SADYYHR--------------------------VSSNEGS----RLGCCCEAVE 521

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy Swiss-Prot
Match: Q8S8Y8 (Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN=WNK6 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 3.1e-58
Identity = 196/637 (30.77%), Postives = 268/637 (42.07%), Query Frame = 0 

Query: 5   GSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIE 64
           G+D  S   E  D  +LE DPT RYIRY E++GKGAFKTV+KAFD+  GIEVAW+QV I+
Sbjct: 3   GTDDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRID 62

Query: 65  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR-- 124
           DVLQSP  L+RLYSEV LLKSLKH NII+FY+ W+DD  KT+N+ITELFTSGSLRH R  
Sbjct: 63  DVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKK 122

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 123 HRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 185 -----------------------------------------------------------L 244
                                                                      +
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKV 242

Query: 245 PEGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL----ATENRKDHSSERS 304
             G+ PASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL     T N      +  
Sbjct: 243 SSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIV 302

Query: 305 MLLNAQF-----ESVNPPLL---DTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQ--- 364
           M     F      S  PP      T  +D+D              +SN  I  FS     
Sbjct: 303 MPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDE-------------DSNLPIVTFSDNSGS 362

Query: 365 ---ELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEM 424
              E++     N   LKG+  D  S+SL LRI + +G+ +NIHF F+ + DTA  ++ EM
Sbjct: 363 RCIEVRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEM 422

Query: 425 VEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFS 484
           VEQLEL++++ T IA+LID L+   +P+W+      D LI  HS                
Sbjct: 423 VEQLELTDQNVTFIAELIDILLVNMIPTWKTDVT-VDHLI--HSQ--------------- 482

Query: 485 EMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFS 503
                       + + +   E + Q+ ++T+        +HS  SD              
Sbjct: 483 ----------LNQNSRSHHNEAKPQKQEETVFHDTCELVSHSCNSDCP-----------R 542

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy Swiss-Prot
Match: Q8LST2 (Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN=WNK7 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 7.7e-57
Identity = 192/639 (30.05%), Postives = 268/639 (41.94%), Query Frame = 0 

Query: 5   GSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIE 64
           GS+  S   E  D  +LE DPT RYIRY E++GKGA KTVFK FD+  GIEVAW+QV I+
Sbjct: 3   GSEDASAIVEPPDPEVLEIDPTCRYIRYKEVIGKGASKTVFKGFDEVDGIEVAWNQVRID 62

Query: 65  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR----- 124
           D+LQSP+ L+RLYSEV LLKSLKH+NII+FY+ W+DD  KT+N+ITELFTSGSLR     
Sbjct: 63  DLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYRKK 122

Query: 125 -----------------------HSRLP-------------------------------- 184
                                  HS+ P                                
Sbjct: 123 HRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYKKV 242

Query: 245 -EGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATEN------------- 304
             G+ PASL KV DP+V +FIEKCL+PAS RL A ELL D FL                 
Sbjct: 243 SSGIKPASLSKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPDIV 302

Query: 305 --RKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQ 364
             ++    ER ++      S  PP      M M+ +      ++   I SNN  S  +  
Sbjct: 303 MPKEGSFGERCLM------SEGPPNARNRTMSMNLD---EDNNLPIVISSNN--SGTNCI 362

Query: 365 ELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQ 424
           E++     N   LKG+  D NS+SL LRI + +G+ +NIHF FF + DTA  ++ EMVEQ
Sbjct: 363 EVRRAKRGNFFVLKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQ 422

Query: 425 LELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMV 484
           LEL++++   IA+LID L+   +P+W+  T+     +     +++ +D            
Sbjct: 423 LELTDKNVKFIAELIDVLLVNLIPNWK--TDVAVDHLIHPQQNQSSKD------------ 482

Query: 485 LSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPE 508
                                  +Q    S A    +HS  SDY              P 
Sbjct: 483 ----------------------NHQNGASSQAGESISHSLSSDYC-------------PR 542

[Close]
BLAST of Cp4.1LG05g07750 vs. NCBI nr
Match: XP_023534400.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 931 bits (2407), Expect = 0.0
Identity = 506/630 (80.32%), Postives = 506/630 (80.32%), Query Frame = 0 

Query: 4   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 63
           CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI
Sbjct: 3   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 62

Query: 64  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR- 123
           EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR  R 
Sbjct: 63  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRQYRK 122

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 123 KHRKVDLKAFKNWARKILRGLTYLHGHDPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIV 182

Query: 184 ----------------LPE----------------------------------------- 243
                            PE                                         
Sbjct: 183 LQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKK 242

Query: 244 ---GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 303
              GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL
Sbjct: 243 VTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 302

Query: 304 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 363
           NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK
Sbjct: 303 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 362

Query: 364 GDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 423
           GDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL
Sbjct: 363 GDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 422

Query: 424 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 483
           IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT
Sbjct: 423 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 482

Query: 484 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 512
           ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD
Sbjct: 483 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 542

[Close]
BLAST of Cp4.1LG05g07750 vs. NCBI nr
Match: XP_022958479.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 502/630 (79.68%), Postives = 502/630 (79.68%), Query Frame = 0 

Query: 4   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 63
           CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI
Sbjct: 3   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 62

Query: 64  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR- 123
           EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR  R 
Sbjct: 63  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRQYRK 122

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 123 KHRKVDLKAFKNWARKILRGLTYLHGQDPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIV 182

Query: 184 ----------------LPE----------------------------------------- 243
                            PE                                         
Sbjct: 183 LQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKK 242

Query: 244 ---GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 303
              GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSE SMLL
Sbjct: 243 VTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSEPSMLL 302

Query: 304 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 363
           NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK
Sbjct: 303 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 362

Query: 364 GDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 423
           GDMTDHNSIS HLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL
Sbjct: 363 GDMTDHNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 422

Query: 424 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 483
           IDELIAKFVPSWQQCTN CDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT
Sbjct: 423 IDELIAKFVPSWQQCTNCCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 482

Query: 484 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 512
           ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSG GTSD
Sbjct: 483 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGSGTSD 542

[Close]
BLAST of Cp4.1LG05g07750 vs. NCBI nr
Match: XP_022996253.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 916 bits (2367), Expect = 0.0
Identity = 497/630 (78.89%), Postives = 501/630 (79.52%), Query Frame = 0 

Query: 4   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 63
           CGSD MSGNSEADD +IL+KDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI
Sbjct: 3   CGSDLMSGNSEADDFDILDKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 62

Query: 64  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR- 123
           EDVLQSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDDNTKTINMITELFTSGSLR  R 
Sbjct: 63  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFFSYWVDDNTKTINMITELFTSGSLRQYRK 122

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 123 KHRKVDLKAFKNWARKILRGLTYLHGHDPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIV 182

Query: 184 ----------------LPE----------------------------------------- 243
                            PE                                         
Sbjct: 183 LQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKK 242

Query: 244 ---GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 303
              GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL
Sbjct: 243 VTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 302

Query: 304 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 363
           NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK
Sbjct: 303 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 362

Query: 364 GDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 423
           GDMTDHNSIS HLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL
Sbjct: 363 GDMTDHNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 422

Query: 424 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 483
           IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT
Sbjct: 423 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 482

Query: 484 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 512
           ELAEVEHQENQQTIISCASLDYNHSTISDYS+GKGGSECGDFSHPECDKAFISSG GTSD
Sbjct: 483 ELAEVEHQENQQTIISCASLDYNHSTISDYSMGKGGSECGDFSHPECDKAFISSGSGTSD 542

[Close]
BLAST of Cp4.1LG05g07750 vs. NCBI nr
Match: KAG7035510.1 (Serine/threonine-protein kinase WNK8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 908 bits (2347), Expect = 0.0
Identity = 501/654 (76.61%), Postives = 502/654 (76.76%), Query Frame = 0 

Query: 4   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTV------------------- 63
           CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKT                    
Sbjct: 3   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTFVNQFRLNIVGSREGILFDL 62

Query: 64  --------FKAFDKAGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYS 123
                   FKAFDKAGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYS
Sbjct: 63  RSVFIIIRFKAFDKAGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYS 122

Query: 124 YWVDDNTKTINMITELFTSGSLRHSR---------------------------------- 183
           YWVDDNTKTINMITELFTSGSLR  R                                  
Sbjct: 123 YWVDDNTKTINMITELFTSGSLRQYRKKHRKVDLKAFKNWARKILRGLTYLHGQDPPIIH 182

Query: 184 -------------------------------------------LPE-------------- 243
                                                       PE              
Sbjct: 183 RDLKCDNIFVNGNTGEVKIGDLGLAIVLQQPTARSVIGTPEFMAPELYDEDYNELVDIYS 242

Query: 244 ---------------------------GVMPASLEKVLDPQVKQFIEKCLVPASMRLPAS 303
                                      GVMPASLEKVLDPQV+QFIEKCLVPASMRLPAS
Sbjct: 243 FGMCMLEIVTCEYPYNECKNSAQIFKKGVMPASLEKVLDPQVRQFIEKCLVPASMRLPAS 302

Query: 304 ELLKDPFLATENRKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIES 363
           ELLKDPFLATENRKDHSSE SMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIES
Sbjct: 303 ELLKDPFLATENRKDHSSEPSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIES 362

Query: 364 NNEISHFSTQELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTA 423
           NNEISHFSTQELKTLTENNELTLKGDMTDHNSIS HLRIAELSGQSKNIHFAFFLDSDTA
Sbjct: 363 NNEISHFSTQELKTLTENNELTLKGDMTDHNSISFHLRIAELSGQSKNIHFAFFLDSDTA 422

Query: 424 LAIALEMVEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKA 483
           LAIALEMVEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKA
Sbjct: 423 LAIALEMVEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKA 482

Query: 484 FSPPLFSEMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGG 512
           FSPPLFSEMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGG
Sbjct: 483 FSPPLFSEMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGG 542

[Close]
BLAST of Cp4.1LG05g07750 vs. NCBI nr
Match: KAG6605599.1 (putative serine/threonine-protein kinase WNK4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 837 bits (2163), Expect = 9.52e-302
Identity = 460/590 (77.97%), Postives = 461/590 (78.14%), Query Frame = 0 

Query: 44  VFKAFDKAGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNT 103
           +FKAFDKAGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNT
Sbjct: 8   IFKAFDKAGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNT 67

Query: 104 KTINMITELFTSGSLRHSR----------------------------------------- 163
           KTINMITELFTSGSLR  R                                         
Sbjct: 68  KTINMITELFTSGSLRQYRKKHRKVDLKAFKNWARKILRGLTYLHGQDPPIIHRDLKCDN 127

Query: 164 ------------------------------------LPE--------------------- 223
                                                PE                     
Sbjct: 128 IFVNGNTGEVKIGDLGLAIVLQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLE 187

Query: 224 -----------------------GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLK 283
                                  GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLK
Sbjct: 188 IVTCEYPYNECKNSAQIFKKVTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLK 247

Query: 284 DPFLATENRKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEI 343
           DPFLATENRKDHSSE SMLLNAQFESVNPPLLDTNPMDM HNCSKLSGSVASSIESNNEI
Sbjct: 248 DPFLATENRKDHSSEPSMLLNAQFESVNPPLLDTNPMDMGHNCSKLSGSVASSIESNNEI 307

Query: 344 SHFSTQELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIA 403
           SHFSTQELKTLTENNELTLKGDMTDHNSIS HLRIAEL GQSKNIHFAFFLDSDTALAIA
Sbjct: 308 SHFSTQELKTLTENNELTLKGDMTDHNSISFHLRIAELCGQSKNIHFAFFLDSDTALAIA 367

Query: 404 LEMVEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPP 463
           LEMVEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPP
Sbjct: 368 LEMVEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPP 427

Query: 464 LFSEMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECG 512
           LFSEMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECG
Sbjct: 428 LFSEMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECG 487

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy TrEMBL
Match: A0A6J1H579 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111459693 PE=4 SV=1)

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 502/630 (79.68%), Postives = 502/630 (79.68%), Query Frame = 0 

Query: 4   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 63
           CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI
Sbjct: 3   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 62

Query: 64  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR- 123
           EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR  R 
Sbjct: 63  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRQYRK 122

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 123 KHRKVDLKAFKNWARKILRGLTYLHGQDPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIV 182

Query: 184 ----------------LPE----------------------------------------- 243
                            PE                                         
Sbjct: 183 LQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKK 242

Query: 244 ---GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 303
              GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSE SMLL
Sbjct: 243 VTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSEPSMLL 302

Query: 304 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 363
           NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK
Sbjct: 303 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 362

Query: 364 GDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 423
           GDMTDHNSIS HLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL
Sbjct: 363 GDMTDHNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 422

Query: 424 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 483
           IDELIAKFVPSWQQCTN CDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT
Sbjct: 423 IDELIAKFVPSWQQCTNCCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 482

Query: 484 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 512
           ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSG GTSD
Sbjct: 483 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGSGTSD 542

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy TrEMBL
Match: A0A6J1KAA6 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111491535 PE=4 SV=1)

HSP 1 Score: 916 bits (2367), Expect = 0.0
Identity = 497/630 (78.89%), Postives = 501/630 (79.52%), Query Frame = 0 

Query: 4   CGSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 63
           CGSD MSGNSEADD +IL+KDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI
Sbjct: 3   CGSDLMSGNSEADDFDILDKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDI 62

Query: 64  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR- 123
           EDVLQSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDDNTKTINMITELFTSGSLR  R 
Sbjct: 63  EDVLQSPEQLQRLYSEVHLLKSLKHENIIKFFSYWVDDNTKTINMITELFTSGSLRQYRK 122

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 123 KHRKVDLKAFKNWARKILRGLTYLHGHDPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIV 182

Query: 184 ----------------LPE----------------------------------------- 243
                            PE                                         
Sbjct: 183 LQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKK 242

Query: 244 ---GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 303
              GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL
Sbjct: 243 VTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLL 302

Query: 304 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 363
           NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK
Sbjct: 303 NAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLK 362

Query: 364 GDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 423
           GDMTDHNSIS HLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL
Sbjct: 363 GDMTDHNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQL 422

Query: 424 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 483
           IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT
Sbjct: 423 IDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLT 482

Query: 484 ELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 512
           ELAEVEHQENQQTIISCASLDYNHSTISDYS+GKGGSECGDFSHPECDKAFISSG GTSD
Sbjct: 483 ELAEVEHQENQQTIISCASLDYNHSTISDYSMGKGGSECGDFSHPECDKAFISSGSGTSD 542

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy TrEMBL
Match: A0A6J1K697 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111491535 PE=4 SV=1)

HSP 1 Score: 743 bits (1918), Expect = 6.36e-266
Identity = 395/466 (84.76%), Postives = 414/466 (88.84%), Query Frame = 0 

Query: 53  GIEVAWSQVDIEDVLQSPEQLQ-RLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITE 112
           G+ +   Q     V+ +PE +   LY E        +  ++  YS+ +      + ++T 
Sbjct: 72  GLAIVLQQPTARSVIGTPEFMAPELYDE-------DYNELVDIYSFGMC----MLEIVTC 131

Query: 113 LFTSGSLRHS-----RLPEGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL 172
            +     ++S     ++  GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL
Sbjct: 132 EYPYNECKNSAQIFKKVTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL 191

Query: 173 ATENRKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFS 232
           ATENRKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFS
Sbjct: 192 ATENRKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFS 251

Query: 233 TQELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMV 292
           TQELKTLTENNELTLKGDMTDHNSIS HLRIAELSGQSKNIHFAFFLDSDTALAIALEMV
Sbjct: 252 TQELKTLTENNELTLKGDMTDHNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMV 311

Query: 293 EQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSE 352
           EQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSE
Sbjct: 312 EQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSE 371

Query: 353 MVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSH 412
           MVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYS+GKGGSECGDFSH
Sbjct: 372 MVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSMGKGGSECGDFSH 431

Query: 413 PECDKAFISSGRGTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQ 472
           PECDKAFISSG GTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQ
Sbjct: 432 PECDKAFISSGSGTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQ 491

Query: 473 HDELKMEIDAIDMQYHQCLCELSRMREEAIESAKKRWMSKKKGAAI 512
           HDELKMEIDAIDMQYHQCLCELSRMREEAIESAK RWMSKKKGAAI
Sbjct: 492 HDELKMEIDAIDMQYHQCLCELSRMREEAIESAKTRWMSKKKGAAI 526

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy TrEMBL
Match: A0A6J1H3J5 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111459693 PE=4 SV=1)

HSP 1 Score: 740 bits (1911), Expect = 7.39e-265
Identity = 395/466 (84.76%), Postives = 413/466 (88.63%), Query Frame = 0 

Query: 53  GIEVAWSQVDIEDVLQSPEQLQ-RLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITE 112
           G+ +   Q     V+ +PE +   LY E        +  ++  YS+ +      + ++T 
Sbjct: 72  GLAIVLQQPTARSVIGTPEFMAPELYDE-------DYNELVDIYSFGMC----MLEIVTC 131

Query: 113 LFTSGSLRHS-----RLPEGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL 172
            +     ++S     ++  GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL
Sbjct: 132 EYPYNECKNSAQIFKKVTSGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL 191

Query: 173 ATENRKDHSSERSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFS 232
           ATENRKDHSSE SMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFS
Sbjct: 192 ATENRKDHSSEPSMLLNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFS 251

Query: 233 TQELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMV 292
           TQELKTLTENNELTLKGDMTDHNSIS HLRIAELSGQSKNIHFAFFLDSDTALAIALEMV
Sbjct: 252 TQELKTLTENNELTLKGDMTDHNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMV 311

Query: 293 EQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSE 352
           EQLELSNEDATIIAQLIDELIAKFVPSWQQCTN CDGLITEHSSSETEEDKAFSPPLFSE
Sbjct: 312 EQLELSNEDATIIAQLIDELIAKFVPSWQQCTNCCDGLITEHSSSETEEDKAFSPPLFSE 371

Query: 353 MVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSH 412
           MVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSH
Sbjct: 372 MVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSH 431

Query: 413 PECDKAFISSGRGTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQ 472
           PECDKAFISSG GTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQ
Sbjct: 432 PECDKAFISSGSGTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQ 491

Query: 473 HDELKMEIDAIDMQYHQCLCELSRMREEAIESAKKRWMSKKKGAAI 512
           HDELKMEIDAIDMQYHQCLCELSRMREEAIESAKKRWMSKKKGAAI
Sbjct: 492 HDELKMEIDAIDMQYHQCLCELSRMREEAIESAKKRWMSKKKGAAI 526

[Close]
BLAST of Cp4.1LG05g07750 vs. ExPASy TrEMBL
Match: A0A5A7TLP6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001600 PE=4 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 4.28e-255
Identity = 404/634 (63.72%), Postives = 450/634 (70.98%), Query Frame = 0 

Query: 4   CGSDF-MSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVD 63
           CGSD  MS NSEADD  I+EKDP+GRYIRYDEILGKGAFKTV+KAFD+ GGIEVAWSQVD
Sbjct: 3   CGSDLIMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVD 62

Query: 64  IEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR 123
           IEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDD  KTINMITELFTSGSLR  R
Sbjct: 63  IEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYR 122

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 123 KKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAI 182

Query: 184 -----------------LPE---------------------------------------- 243
                             PE                                        
Sbjct: 183 VMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFK 242

Query: 244 ----GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSML 303
               G+ PASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  E+ K+HSSE S  
Sbjct: 243 KVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRS 302

Query: 304 LNAQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTL 363
           LN QF+SVNPPLL ++PMD DH C+KLSGSVASS++SNN ISHFSTQEL+ LTENNELTL
Sbjct: 303 LNEQFKSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTL 362

Query: 364 KGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQ 423
           KG+MTDHN++S HLRIAEL G+S+NIHFAF+LDSDT+LAIALEMVEQLELSNEDATIIA+
Sbjct: 363 KGEMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAK 422

Query: 424 LIDELIAKFVPSWQQCTNFCDG--LITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRK 483
           LIDELIAKFVPSW+ C N+C+     T  S SE +E+K+F  P FSE+VLSSP+V A R 
Sbjct: 423 LIDELIAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARN 482

Query: 484 TLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRG 512
            LT LA+VE QENQQ+IISCAS++Y +ST+SDYS+GKG SECG+F HP+C+KA+ISSG  
Sbjct: 483 NLTGLAKVEDQENQQSIISCASVEYIYSTVSDYSIGKG-SECGEFGHPDCEKAYISSGTI 542

[Close]
BLAST of Cp4.1LG05g07750 vs. TAIR 10
Match: AT5G41990.1 (with no lysine (K) kinase 8 )

HSP 1 Score: 287.7 bits (735), Expect = 1.8e-77
Identity = 216/628 (34.39%), Postives = 305/628 (48.57%), Query Frame = 0 

Query: 5   GSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIE 64
           GS F+   S  ++ +  EKDP+GRYIRYD++LG+GAFKTV+KAFD+  GIEVAW+ V IE
Sbjct: 4   GSGFLGQISSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIE 63

Query: 65  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR----- 124
           DV+Q P QL+RLYSEVHLLK+LKHENIIK +  WVD+  KTINMITELFTSGSLR     
Sbjct: 64  DVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKK 123

Query: 125 -----------------------HSRLP-------------------------------- 184
                                  HS+ P                                
Sbjct: 124 HRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVL 183

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 184 QQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKV 243

Query: 245 -EGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLLN 304
              + P SL KV DPQV+QFIEKCL+PAS R  A EL KDPFLA +  KD +   S   +
Sbjct: 244 TSNIKPQSLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSALLASSSTS 303

Query: 305 AQFESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLKG 364
           +++  V PP L+  PMD+DHN +K       S+ SN +     T EL+ + EN E  L+G
Sbjct: 304 SKY--VRPPQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFRLRG 363

Query: 365 DMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLI 424
           + +D  + S+ LRIA+ SG+ + +HFAF+L+SDTA AIA EMVE+L L++++  +IA +I
Sbjct: 364 ERSDDVTASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMI 423

Query: 425 DELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTE 484
           D+ I +              L+++ +SS   ++   SP L  E                 
Sbjct: 424 DDFIMQ--------------LLSDRTSSHHNQN---SPRLTHE----------------- 483

Query: 485 LAEVEHQ-ENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSD 511
               +H+  NQQT+ S        S  SD S         D+  P       S+  G + 
Sbjct: 484 ----DHEAANQQTVNSKDEEAAGQSMKSDIS--------ADYYFP------YSANDGNAA 543

[Close]
BLAST of Cp4.1LG05g07750 vs. TAIR 10
Match: AT1G64630.1 (with no lysine (K) kinase 10 )

HSP 1 Score: 250.8 bits (639), Expect = 2.4e-66
Identity = 196/610 (32.13%), Postives = 272/610 (44.59%), Query Frame = 0 

Query: 16  DDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIEDVLQSPEQLQR 75
           ++ + ++KDPTGRYIRY+++LG+GAFKTV+KAFD+  GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 76  LYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLR---------------- 135
           LYSEVHLL SLKH+NIIK +  WVDD+ K+INMITELFTSGSL                 
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 136 ------------HSRLP------------------------------------------- 195
                       HS+ P                                           
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 196 --------------------------------------------------EGVMPASLEK 255
                                                              G+ P SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 256 VLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSERSMLLNAQFESVNPPLL 315
           V DPQVKQFIEKCL+PA  R  A ELLKD  LA +  KD +   S   N  F+   PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTLTASS--NTTFKPAMPPQC 301

Query: 316 DTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLKGDMTDHNSISLH 375
           +  PMD+++     S S+ SS +S+ E +   T E++ + E+ E  L G+  D  + S+ 
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 376 LRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLIDELIAKFVPSW 435
           LRIA  SGQ++ + F F L +DTA A+  EMVE+L+LS+ + T+IA++IDELI K   + 
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMKLKAN- 421

Query: 436 QQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTELAEVEHQENQQ 495
                    L   +S  ++++++                  AG    +E+          
Sbjct: 422 -------RSLPNANSVYQSKDEE------------------AGESMKSEI---------- 481

Query: 496 TIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGRGTSDLDAVGSVSTMFD 505
                 S DY H                           +SS  G+     +G      +
Sbjct: 482 ------SADYYHR--------------------------VSSNEGS----RLGCCCEAVE 521

[Close]
BLAST of Cp4.1LG05g07750 vs. TAIR 10
Match: AT3G18750.2 (with no lysine (K) kinase 6 )

HSP 1 Score: 233.0 bits (593), Expect = 5.3e-61
Identity = 191/570 (33.51%), Postives = 261/570 (45.79%), Query Frame = 0 

Query: 5   GSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIE 64
           G+D  S   E  D  +LE DPT RYIRY E++GKGAFKTV+KAFD+  GIEVAW+QV I+
Sbjct: 3   GTDDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRID 62

Query: 65  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSRLP 124
           DVLQSP  L+RLYSEV LLKSLKH NII+FY+ W+DD  KT+N+ITELFTSGSLRH R  
Sbjct: 63  DVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKK 122

Query: 125 -----------------------EGVMPASLEKVLDPQ---------------------- 184
                                   G  P  + + L                         
Sbjct: 123 HRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 185 ---------VKQFIEKCLVPASMRLPASELLKDPFL----ATENRKDHSSERSMLLNAQF 244
                    VKQFIEKCL+PAS RL A ELL DPFL     T N      +  M     F
Sbjct: 183 EQANAKSVIVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKEGAF 242

Query: 245 -----ESVNPPLL---DTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQ------ELKT 304
                 S  PP      T  +D+D              +SN  I  FS        E++ 
Sbjct: 243 GDRCLMSEGPPTTRPSKTLSIDLDE-------------DSNLPIVTFSDNSGSRCIEVRR 302

Query: 305 LTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELS 364
               N   LKG+  D  S+SL LRI + +G+ +NIHF F+ + DTA  ++ EMVEQLEL+
Sbjct: 303 AKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELT 362

Query: 365 NEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFSEMVLSSP 424
           +++ T IA+LID L+   +P+W+      D LI  HS                       
Sbjct: 363 DQNVTFIAELIDILLVNMIPTWKTDVT-VDHLI--HSQ---------------------- 422

Query: 425 LVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKA 484
                + + +   E + Q+ ++T+        +HS  SD                E DK 
Sbjct: 423 ---LNQNSRSHHNEAKPQKQEETVFHDTCELVSHSCNSDCP-----------RSDEEDKQ 482

Query: 485 FISSGRGTSDLDAVGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQHDELKM 503
            + + +G                   S I      ++ +SL           ++ + L+ 
Sbjct: 483 CVDATKGED----------------KSSIQEVEEATEPVSL-----------EEEERLRQ 493

[Close]
BLAST of Cp4.1LG05g07750 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 227.6 bits (579), Expect = 2.2e-59
Identity = 196/637 (30.77%), Postives = 268/637 (42.07%), Query Frame = 0 

Query: 5   GSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIE 64
           G+D  S   E  D  +LE DPT RYIRY E++GKGAFKTV+KAFD+  GIEVAW+QV I+
Sbjct: 3   GTDDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRID 62

Query: 65  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR-- 124
           DVLQSP  L+RLYSEV LLKSLKH NII+FY+ W+DD  KT+N+ITELFTSGSLRH R  
Sbjct: 63  DVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKK 122

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 123 HRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 185 -----------------------------------------------------------L 244
                                                                      +
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKV 242

Query: 245 PEGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL----ATENRKDHSSERS 304
             G+ PASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL     T N      +  
Sbjct: 243 SSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIV 302

Query: 305 MLLNAQF-----ESVNPPLL---DTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQ--- 364
           M     F      S  PP      T  +D+D              +SN  I  FS     
Sbjct: 303 MPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDE-------------DSNLPIVTFSDNSGS 362

Query: 365 ---ELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEM 424
              E++     N   LKG+  D  S+SL LRI + +G+ +NIHF F+ + DTA  ++ EM
Sbjct: 363 RCIEVRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEM 422

Query: 425 VEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFS 484
           VEQLEL++++ T IA+LID L+   +P+W+      D LI  HS                
Sbjct: 423 VEQLELTDQNVTFIAELIDILLVNMIPTWKTDVT-VDHLI--HSQ--------------- 482

Query: 485 EMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFS 503
                       + + +   E + Q+ ++T+        +HS  SD              
Sbjct: 483 ----------LNQNSRSHHNEAKPQKQEETVFHDTCELVSHSCNSDCP-----------R 542

[Close]
BLAST of Cp4.1LG05g07750 vs. TAIR 10
Match: AT3G18750.3 (with no lysine (K) kinase 6 )

HSP 1 Score: 227.6 bits (579), Expect = 2.2e-59
Identity = 196/637 (30.77%), Postives = 268/637 (42.07%), Query Frame = 0 

Query: 5   GSDFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIE 64
           G+D  S   E  D  +LE DPT RYIRY E++GKGAFKTV+KAFD+  GIEVAW+QV I+
Sbjct: 3   GTDDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRID 62

Query: 65  DVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRHSR-- 124
           DVLQSP  L+RLYSEV LLKSLKH NII+FY+ W+DD  KT+N+ITELFTSGSLRH R  
Sbjct: 63  DVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKK 122

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 123 HRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVM 182

Query: 185 -----------------------------------------------------------L 244
                                                                      +
Sbjct: 183 EQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKV 242

Query: 245 PEGVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL----ATENRKDHSSERS 304
             G+ PASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL     T N      +  
Sbjct: 243 SSGIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIV 302

Query: 305 MLLNAQF-----ESVNPPLL---DTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQ--- 364
           M     F      S  PP      T  +D+D              +SN  I  FS     
Sbjct: 303 MPKEGAFGDRCLMSEGPPTTRPSKTLSIDLDE-------------DSNLPIVTFSDNSGS 362

Query: 365 ---ELKTLTENNELTLKGDMTDHNSISLHLRIAELSGQSKNIHFAFFLDSDTALAIALEM 424
              E++     N   LKG+  D  S+SL LRI + +G+ +NIHF F+ + DTA  ++ EM
Sbjct: 363 RCIEVRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEM 422

Query: 425 VEQLELSNEDATIIAQLIDELIAKFVPSWQQCTNFCDGLITEHSSSETEEDKAFSPPLFS 484
           VEQLEL++++ T IA+LID L+   +P+W+      D LI  HS                
Sbjct: 423 VEQLELTDQNVTFIAELIDILLVNMIPTWKTDVT-VDHLI--HSQ--------------- 482

Query: 485 EMVLSSPLVGAGRKTLTELAEVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFS 503
                       + + +   E + Q+ ++T+        +HS  SD              
Sbjct: 483 ----------LNQNSRSHHNEAKPQKQEETVFHDTCELVSHSCNSDCP-----------R 542

The following BLAST results are available for this feature:
BLAST of Cp4.1LG05g07750 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q944Q02.5e-7634.39Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=... [more]
Q6EU495.3e-7434.78Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX... [more]
Q8RXE53.4e-6532.13Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8S8Y83.1e-5830.77Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8LST27.7e-5730.05Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN... [more]
BLAST of Cp4.1LG05g07750 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023534400.10.080.32serine/threonine-protein kinase WNK8-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
XP_022958479.10.079.68serine/threonine-protein kinase WNK8-like isoform X1 [Cucurbita moschata][more]
XP_022996253.10.078.89serine/threonine-protein kinase WNK8-like isoform X1 [Cucurbita maxima][more]
KAG7035510.10.076.61Serine/threonine-protein kinase WNK8 [Cucurbita argyrosperma subsp. argyrosperma... [more]
KAG6605599.19.52e-30277.97putative serine/threonine-protein kinase WNK4, partial [Cucurbita argyrosperma s... [more]
BLAST of Cp4.1LG05g07750 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1H5790.079.68Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KAA60.078.89Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1K6976.36e-26684.76Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1H3J57.39e-26584.76Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A5A7TLP64.28e-25563.72Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
BLAST of Cp4.1LG05g07750 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G41990.11.8e-7734.39with no lysine (K) kinase 8 [more]
AT1G64630.12.4e-6632.13with no lysine (K) kinase 10 [more]
AT3G18750.25.3e-6133.51with no lysine (K) kinase 6 [more]
AT3G18750.12.2e-5930.77with no lysine (K) kinase 6 [more]
AT3G18750.32.2e-5930.77with no lysine (K) kinase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 30..166
e-value: 9.1E-5
score: -68.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 33..120
e-value: 8.5E-11
score: 41.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 30..344
score: 8.48536
NoneNo IPR availableGENE3D3.10.20.90coord: 249..340
e-value: 1.2E-7
score: 33.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 13..111
e-value: 3.3E-35
score: 122.0
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 125..507
NoneNo IPR availablePANTHERPTHR13902:SF139WNK KINASEcoord: 12..122
NoneNo IPR availablePANTHERPTHR13902:SF139WNK KINASEcoord: 125..507
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 12..122
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 251..308
e-value: 2.8E-6
score: 27.3
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 24..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g07750.1Cp4.1LG05g07750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity