Cp4.1LG05g03750 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG05g03750
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionheat stress transcription factor B-2b-like
LocationCp4.1LG05: 1741469 .. 1743848 (-)
RNA-Seq ExpressionCp4.1LG05g03750
SyntenyCp4.1LG05g03750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCGTCGCCTGCGGAGCCGATAGGCGATTCCGGAAACGGAGATTCTCAGAGATCTATTCCGACGCCGTTTCTAACAAAAACGTATCAACTCGTTGATGATCCGGATGTCGACGACCTCATCTCGTGGAACGAAGATGGATCTACCTTCATAGTCTGGCGACCTGCTGAATTCGCTCGAGATTTACTTCCTAAATACTTTAAACACAATAACTTTTCCAGTTTCGTCCGTCAACTTAACACTTACGTAAGTTTCTGCATCTTTCGACTGTTTGCCAAATTTCGAATCGGCTTTACTTTATTTTTGGATTCACTATTTTTCTTGGAAAAACGGATGATGGTTCTGTTTTATATATCTTTAATTGCCAGAATTTTGTTTTTAATTTTATATTTTCGTAACTACCGCGCAGGGATTCCGGAAGGTTGTGCCGGATCGATGGGAATTTGCGAACGATTGTTTCAAGAGAGGTGAAAAAGCACTTCTCCGAGACATCCAGCGACGGAAGGTAGCAGTGTCGGTGGCGGCTCCGACGACGCCGGCCTCCGTCTCTGTACCACTGGTGACAGTCGCAGCGTCTCCGGCGGTGGCGGCGTGCGTGATATCGCCGGTGAATTCTGCCGAAGAGCAGGTGACGTCCTCGAACTCATCGCCGATGGAATTCCAGCGAGGTACGAGCTGCTGCACTACACCAGAACTGGTGAGAGAGAACGAGCGGCTGAGGAAGGAGAACATGCAACTGAGTCACGAGTTGACTCAGTTGAAAGGGCTCTGCAACAACATACTATCGTTGATGACGAATTACGCTTCAGGTCACCAGCAGTTGGAGTCGGTGAGCGTCCGGGACGGGAAGGCGCTAGACCTCTTGCCAGCAAGGCAGATGATGGATGACGAAGGCGCCGTCAGCGACGGGATTCAGGAAGTGAGACTGAAGGTGGAGGAGGCGGTGGCGACGGCGACGGCGGAGGGAAGGACGCCGAAGCTGTTCGGAGTTTCTATTGGAGTGAAGCGCGTGAGAAGAGAAGAAGAAGATGAAGAAATGGTGGGGCCGAATCACGTGCAGTCGGAAGAATGTGAGACCGGGTCGGAGATTAAAGCTGAGCCGTTGGATGAGAACTCTGAAAATCCAGAGGGATCCGCATCGCAATGGCTAGAACTCGGAAATCAAGGCTCCTAAATGGCGTCGTCAGAGATGACTGACAAAGAAGAAGAAGAAGACGACGACGACGACGACGTCGACGATAATGTTTTCTTCTAAAAAAAAAAAGAATAAAGAGAGAATGCAGAGACAAGAGGAGACCAACCCGGCCCTAGAGAGCACGTAGAATCTCACGTGTCGAGCACGTGAGATGGAAAATACACGAACTGAAAATAAATATTTGTTTTTTGTTCGTGAAAGGGTTTCAGATCGGTTGATTGGTTGGTCCCTTGTAAAGGATGAAGCCGTGGAAAATCAACCGGCTGGGTCGGTTGGTGAAGGGGATGAAATGACGGAAAAACCAACCCAGGAAGAACGGCAATTGTGACTTTGTAATTTTCAGTTTGTAACCTCTCTTCCAATTTCTATCTTTCAGAAATTTAATTTAAAGGCAAAAAAGGAAGGAAAAGAACAACCGTCGGTTGTGTGGTTCTTTTGTTTTCCGGTATCCGGTGGGCCGATTGGAATGTGAGGTGTAATTTGTATGGGCTGTTTATGAGCCCACTGTAATGTTCCTCTTCAATTTTGGGTTACTTTCAGTAACATGCTTGGATCTTACTTAAACTTCAAAACAAAAACAAAATTATGTATTCTAGGGGCGGATGGGTGTAAGGATGCGTTCATGAAGCGCGGTGGCCCAGAGTCCAGACCATTGAGACACACGGGCATAGCCACCACTACCACATGGAATACGACTTTCAGCATTCCAAAGTAAGTCAGAGACCTTTCTATCCCATATTAATTTCTTGGAATGCTTCATACGTTATAGGCTAATTGACAACCTTAGATGTATATGCCATTTCTTAGCTATTTAATTAATCTTACTTAGCAATCACACTTTTTGTTATCATTCCATTATTATTTTTATTATTTTTATTATTTTTATTGTCAATACGAACATAATTGATATCTAATCTTTTTTTTTTCAGGAGTACAATAGTATACAATTCACTTTTTATTTTTTGTTTAGTTCAGGTGAAGAATGTTTTATCCATTAAATTATGTGTAGATGAGTATTTACTAATTGACTGGATTCCTTAAACTTTGAATCATTTTATTTATTTTAACTATATGAAAATTCCTTATAGGTATATGTTTTTGCGTGTTTTATTCCATTTTGCTTTTAGGAAAAGAAAAATTGACTACACTAAGATAGATTCTAATGCAAAGATTATAAACTAG

mRNA sequence

ATGGCTCCGTCGCCTGCGGAGCCGATAGGCGATTCCGGAAACGGAGATTCTCAGAGATCTATTCCGACGCCGTTTCTAACAAAAACGTATCAACTCGTTGATGATCCGGATGTCGACGACCTCATCTCGTGGAACGAAGATGGATCTACCTTCATAGTCTGGCGACCTGCTGAATTCGCTCGAGATTTACTTCCTAAATACTTTAAACACAATAACTTTTCCAGTTTCGTCCGTCAACTTAACACTTACGGATTCCGGAAGGTTGTGCCGGATCGATGGGAATTTGCGAACGATTGTTTCAAGAGAGGTGAAAAAGCACTTCTCCGAGACATCCAGCGACGGAAGGTAGCAGTGTCGGTGGCGGCTCCGACGACGCCGGCCTCCGTCTCTGTACCACTGGTGACAGTCGCAGCGTCTCCGGCGGTGGCGGCGTGCGTGATATCGCCGGTGAATTCTGCCGAAGAGCAGGTGACGTCCTCGAACTCATCGCCGATGGAATTCCAGCGAGGTACGAGCTGCTGCACTACACCAGAACTGGTGAGAGAGAACGAGCGGCTGAGGAAGGAGAACATGCAACTGAGTCACGAGTTGACTCAGTTGAAAGGGCTCTGCAACAACATACTATCGTTGATGACGAATTACGCTTCAGGTCACCAGCAGTTGGAGTCGGTGAGCGTCCGGGACGGGAAGGCGCTAGACCTCTTGCCAGCAAGGCAGATGATGGATGACGAAGGCGCCGTCAGCGACGGGATTCAGGAAGTGAGACTGAAGGTGGAGGAGGCGGTGGCGACGGCGACGGCGGAGGGAAGGACGCCGAAGCTGTTCGGAGTTTCTATTGGAGTGAAGCGCGTGAGAAGAGAAGAAGAAGATGAAGAAATGGTGGGGCCGAATCACGTGCAGTCGGAAGAATGGGCGGATGGGTGTAAGGATGCGTTCATGAAGCGCGGTGGCCCAGAGTCCAGACCATTGAGACACACGGGCATAGCCACCACTACCACATGGAATACGACTTTCAGCATTCCAAAGAAAAGAAAAATTGACTACACTAAGATAGATTCTAATGCAAAGATTATAAACTAG

Coding sequence (CDS)

ATGGCTCCGTCGCCTGCGGAGCCGATAGGCGATTCCGGAAACGGAGATTCTCAGAGATCTATTCCGACGCCGTTTCTAACAAAAACGTATCAACTCGTTGATGATCCGGATGTCGACGACCTCATCTCGTGGAACGAAGATGGATCTACCTTCATAGTCTGGCGACCTGCTGAATTCGCTCGAGATTTACTTCCTAAATACTTTAAACACAATAACTTTTCCAGTTTCGTCCGTCAACTTAACACTTACGGATTCCGGAAGGTTGTGCCGGATCGATGGGAATTTGCGAACGATTGTTTCAAGAGAGGTGAAAAAGCACTTCTCCGAGACATCCAGCGACGGAAGGTAGCAGTGTCGGTGGCGGCTCCGACGACGCCGGCCTCCGTCTCTGTACCACTGGTGACAGTCGCAGCGTCTCCGGCGGTGGCGGCGTGCGTGATATCGCCGGTGAATTCTGCCGAAGAGCAGGTGACGTCCTCGAACTCATCGCCGATGGAATTCCAGCGAGGTACGAGCTGCTGCACTACACCAGAACTGGTGAGAGAGAACGAGCGGCTGAGGAAGGAGAACATGCAACTGAGTCACGAGTTGACTCAGTTGAAAGGGCTCTGCAACAACATACTATCGTTGATGACGAATTACGCTTCAGGTCACCAGCAGTTGGAGTCGGTGAGCGTCCGGGACGGGAAGGCGCTAGACCTCTTGCCAGCAAGGCAGATGATGGATGACGAAGGCGCCGTCAGCGACGGGATTCAGGAAGTGAGACTGAAGGTGGAGGAGGCGGTGGCGACGGCGACGGCGGAGGGAAGGACGCCGAAGCTGTTCGGAGTTTCTATTGGAGTGAAGCGCGTGAGAAGAGAAGAAGAAGATGAAGAAATGGTGGGGCCGAATCACGTGCAGTCGGAAGAATGGGCGGATGGGTGTAAGGATGCGTTCATGAAGCGCGGTGGCCCAGAGTCCAGACCATTGAGACACACGGGCATAGCCACCACTACCACATGGAATACGACTTTCAGCATTCCAAAGAAAAGAAAAATTGACTACACTAAGATAGATTCTAATGCAAAGATTATAAACTAG

Protein sequence

MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSVAAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQMMDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQSEEWADGCKDAFMKRGGPESRPLRHTGIATTTTWNTTFSIPKKRKIDYTKIDSNAKIIN
Homology
BLAST of Cp4.1LG05g03750 vs. ExPASy Swiss-Prot
Match: Q9T0D3 (Heat stress transcription factor B-2b OS=Arabidopsis thaliana OX=3702 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.4e-89
Identity = 184/296 (62.16%), Postives = 210/296 (70.95%), Query Frame = 0

Query: 10  GDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFK 69
           G  G GDSQRSIPTPFLTKTYQLV+DP  D+LISWNEDG+TFIVWRPAEFARDLLPKYFK
Sbjct: 45  GGGGGGDSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFK 104

Query: 70  HNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKV---AVSVAAPTTP 129
           HNNFSSFVRQLNTYGFRKVVPDRWEF+NDCFKRGEK LLRDIQRRK+   A++ AA    
Sbjct: 105 HNNFSSFVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAA 164

Query: 130 ASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPM-------------EFQRGTSC 189
           A+V+   VTVAA P VA  ++SP NS EEQV SSNSSP                QR TSC
Sbjct: 165 AAVAASAVTVAAVPVVAH-IVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSC 224

Query: 190 CTTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALD 249
            T PELV ENERLRK+N +L  E+T+LKGL  NI +LM N+  G +    + + +GK LD
Sbjct: 225 TTAPELVEENERLRKDNERLRKEMTKLKGLYANIYTLMANFTPGQEDCAHL-LPEGKPLD 284

Query: 250 LLPARQMMDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEE 290
           LLP RQ M +    S+        +E  +     E  TP+LFGVSIGVKR RREEE
Sbjct: 285 LLPERQEMSEAIMASE--------IETGIGLKLGEDLTPRLFGVSIGVKRARREEE 330

BLAST of Cp4.1LG05g03750 vs. ExPASy Swiss-Prot
Match: Q652B0 (Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2C PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.4e-68
Identity = 159/302 (52.65%), Postives = 193/302 (63.91%), Query Frame = 0

Query: 18  QRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSFV 77
           QRS+PTPFLTKTYQLV+DP VDD+ISWNEDGSTF+VWRPAEFARDLLPKYFKHNNFSSFV
Sbjct: 32  QRSLPTPFLTKTYQLVEDPAVDDVISWNEDGSTFVVWRPAEFARDLLPKYFKHNNFSSFV 91

Query: 78  RQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRK-VAVSVAAPTTP--------AS 137
           RQLNTYGFRK+VPDRWEFANDCF+RGEK LL DI RRK VA + AAP  P        A+
Sbjct: 92  RQLNTYGFRKIVPDRWEFANDCFRRGEKRLLCDIHRRKVVAAAAAAPPPPSPGMATAAAA 151

Query: 138 VSVPLVTVAASPAVAACVI----SPVNSAEEQVTSSNSSPMEFQR------------GTS 197
           V+   VTVAA+P   A  +    SP +S+EEQV SSNS   E  R            G  
Sbjct: 152 VASGAVTVAAAPIPMALPVTRAGSPAHSSEEQVLSSNSGSGEEHRQASGSGSAPGGGGGG 211

Query: 198 CCTTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYAS-----GHQQLESVSVR 257
             +  ++  ENERLR+EN +L+ EL  +K LCNNIL LM+ YA+     G   + S++  
Sbjct: 212 SASGGDMGEENERLRRENARLTRELGHMKKLCNNILLLMSKYAATQHVEGSAGISSIANC 271

Query: 258 DGKALDLLPARQMMDDEGAVSDGIQEVRLKVEEAVATATAEGRTP----KLFGVSIGVKR 286
            G++ + +P    +    A+ D +         A A   A    P    +LFGVSIG+KR
Sbjct: 272 SGESSEAVPPPPPLPP--AILDLMPSCPALATAAAAAGLAIDGEPDPSARLFGVSIGLKR 331

BLAST of Cp4.1LG05g03750 vs. ExPASy Swiss-Prot
Match: Q6Z9C8 (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.8e-68
Identity = 166/341 (48.68%), Postives = 205/341 (60.12%), Query Frame = 0

Query: 3   PSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARD 62
           P+PA      G G  QR++PTPFLTKTYQLVDDP VDD+ISWN+DGSTF+VWRPAEFARD
Sbjct: 27  PAPAAEAAGVGVGQQQRTVPTPFLTKTYQLVDDPAVDDVISWNDDGSTFVVWRPAEFARD 86

Query: 63  LLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSVAA 122
           LLPKYFKHNNFSSFVRQLNTYGFRK+VPDRWEFANDCF+RGE+ LL +I RRKV     A
Sbjct: 87  LLPKYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRKVTPPAPA 146

Query: 123 PTTPA-SVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSP--------MEFQRGTSC 182
            TT A + ++P+    A P       SPV S EEQV SS+SSP             G+  
Sbjct: 147 ATTAAVAAAIPM----ALPVTTTRDGSPVLSGEEQVISSSSSPEPPLVLPQAPSGSGSGG 206

Query: 183 CTTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYAS-------------GHQQ 242
             + ++  ENERLR+EN QL+ EL+Q++ LCNNIL LM+ YAS             G+  
Sbjct: 207 VASGDVGDENERLRRENAQLARELSQMRKLCNNILLLMSKYASTQQLDAANASSAAGNNN 266

Query: 243 LESVSVRDGKALDLLPARQMMD----DEGAVSDGIQEVRLKVEEAVATATAEG-RTPKLF 302
             + S    +A   LP   ++D      GA S            A  +   EG  + KLF
Sbjct: 267 NNNCSGESAEAATPLPLPAVLDLMPSCPGAAS----------AAAPVSDNEEGMMSAKLF 326

Query: 303 GVSIGVKRVRRE---EED-------EEMVGPNHVQSEEWAD 307
           GVSIG KR+R +   ++D       E M G  H + E+ A+
Sbjct: 327 GVSIGRKRMRHDGGGDDDHAATVKAEPMDGRPHGKDEQSAE 353

BLAST of Cp4.1LG05g03750 vs. ExPASy Swiss-Prot
Match: Q9SCW4 (Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.1e-62
Identity = 143/305 (46.89%), Postives = 189/305 (61.97%), Query Frame = 0

Query: 12  SGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHN 71
           +G   SQRSIPTPFLTKT+ LV+D  +DD+ISWNEDGS+FIVW P +FA+DLLPK+FKHN
Sbjct: 11  TGESSSQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHN 70

Query: 72  NFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSVAAPTTPASVSV 131
           NFSSFVRQLNTYGF+KVVPDRWEF+ND FKRGEK LLR+IQRRK+       TT  +V  
Sbjct: 71  NFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKIT------TTHQTVVA 130

Query: 132 PLVTVAASPAVAACVISPVNSAEE----QVTSSNSSPMEFQRGTSCCT---TPELVRENE 191
           P    ++       V+SP NS E+    QV SS+ S     +  +      + EL+ ENE
Sbjct: 131 P----SSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENE 190

Query: 192 RLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSV--RDGKALDLLPARQMMD 251
           +LR +N+QL+ ELTQ+K +C+NI SLM+NY        S S      + ++ LPA++  +
Sbjct: 191 KLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKRFSE 250

Query: 252 DEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRRE---EEDEEMVGPNHV 305
                        +++EE       E  +P+LFGV IG+KR R E    +   +VG N  
Sbjct: 251 -------------MEIEE------EEEASPRLFGVPIGLKRTRSEGVQVKTTAVVGENSD 286

BLAST of Cp4.1LG05g03750 vs. ExPASy Swiss-Prot
Match: P22335 (Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.7e-51
Identity = 124/285 (43.51%), Postives = 165/285 (57.89%), Query Frame = 0

Query: 17  SQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSF 76
           SQR+ P PFL KTYQLVDD   DD+ISWNE G+TF+VW+ AEFA+DLLPKYFKHNNFSSF
Sbjct: 2   SQRTAPAPFLLKTYQLVDDAATDDVISWNEIGTTFVVWKTAEFAKDLLPKYFKHNNFSSF 61

Query: 77  VRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSVAAPTTPASVSVPLVTV 136
           VRQLNTYGFRK+VPD+WEFAN+ FKRG+K LL  I+RRK   S     TPA         
Sbjct: 62  VRQLNTYGFRKIVPDKWEFANENFKRGQKELLTAIRRRKTVTS-----TPA--------- 121

Query: 137 AASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTP-------ELVRENERLRKE 196
                 A    SP NS ++  +SS SSP     G+    TP       +L  ENE+L+K+
Sbjct: 122 GGKSVAAGASASPDNSGDDIGSSSTSSPDSKNPGS--VDTPGKLSQFTDLSDENEKLKKD 181

Query: 197 NMQLSHELTQLKGLCNNILSLMTNYASGH----QQLESVSVRDGKALDLLPARQMMDDEG 256
           N  LS EL Q K  CN +++ ++ Y         ++ S     G +L+     +++ + G
Sbjct: 182 NQMLSSELVQAKKQCNELVAFLSQYVKVAPDMINRIMSQGTPSGSSLE-----ELVKEVG 241

Query: 257 AVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEED 291
            V D  ++      +       +G T KLFGV +  K+ +R  ++
Sbjct: 242 GVKDLEEQGSYNDNDDKEDDDEKGDTLKLFGVLLKEKKKKRGPDE 265

BLAST of Cp4.1LG05g03750 vs. NCBI nr
Match: XP_023521234.1 (heat stress transcription factor B-2b-like [Cucurbita pepo subsp. pepo] >XP_023521235.1 heat stress transcription factor B-2b-like [Cucurbita pepo subsp. pepo] >XP_023534265.1 heat stress transcription factor B-2b-like [Cucurbita pepo subsp. pepo] >XP_023534267.1 heat stress transcription factor B-2b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595 bits (1533), Expect = 1.24e-212
Identity = 304/309 (98.38%), Postives = 305/309 (98.71%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120

Query: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180
           AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV
Sbjct: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180

Query: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240
           RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM
Sbjct: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240

Query: 241 MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300
           MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ
Sbjct: 241 MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300

Query: 301 SEEWADGCK 309
           SEE   G +
Sbjct: 301 SEECETGSE 309

BLAST of Cp4.1LG05g03750 vs. NCBI nr
Match: KAG6606116.1 (Heat stress transcription factor B-2b, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036060.1 Heat stress transcription factor B-2b [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 588 bits (1517), Expect = 3.39e-210
Identity = 301/309 (97.41%), Postives = 302/309 (97.73%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120

Query: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180
           AAP TPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV
Sbjct: 121 AAPATPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180

Query: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240
           RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM
Sbjct: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240

Query: 241 MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300
           MDDEGAVSDGIQEVRLKVEEAVA ATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ
Sbjct: 241 MDDEGAVSDGIQEVRLKVEEAVAVATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300

Query: 301 SEEWADGCK 309
           SEE   G +
Sbjct: 301 SEECETGSE 309

BLAST of Cp4.1LG05g03750 vs. NCBI nr
Match: XP_022958418.1 (heat stress transcription factor B-2b-like [Cucurbita moschata] >XP_022958419.1 heat stress transcription factor B-2b-like [Cucurbita moschata] >XP_022958420.1 heat stress transcription factor B-2b-like [Cucurbita moschata])

HSP 1 Score: 586 bits (1511), Expect = 2.78e-209
Identity = 299/309 (96.76%), Postives = 302/309 (97.73%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVA+SV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVALSV 120

Query: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180
           AAP TPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV
Sbjct: 121 AAPATPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180

Query: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240
           RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM
Sbjct: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240

Query: 241 MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300
           MDDEGAVSDGIQEVRLKVEEAVA ATAEGRTPKLFGVSIGVKRVRREE+DEEMVGPNHVQ
Sbjct: 241 MDDEGAVSDGIQEVRLKVEEAVAVATAEGRTPKLFGVSIGVKRVRREEDDEEMVGPNHVQ 300

Query: 301 SEEWADGCK 309
           SEE   G +
Sbjct: 301 SEECETGSE 309

BLAST of Cp4.1LG05g03750 vs. NCBI nr
Match: XP_022996371.1 (heat stress transcription factor B-2b-like [Cucurbita maxima] >XP_022996372.1 heat stress transcription factor B-2b-like [Cucurbita maxima])

HSP 1 Score: 569 bits (1467), Expect = 1.21e-202
Identity = 294/309 (95.15%), Postives = 297/309 (96.12%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120

Query: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180
           AAP TPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQR TSCCTTPELV
Sbjct: 121 AAPATPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRVTSCCTTPELV 180

Query: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240
           RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKAL+LLPARQM
Sbjct: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALELLPARQM 240

Query: 241 MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300
           MDDEGAVSDGIQEVRLKVEEAVA    EGRTPKLFGVSIGVKRVRREEEDEEM+GPNHVQ
Sbjct: 241 MDDEGAVSDGIQEVRLKVEEAVA----EGRTPKLFGVSIGVKRVRREEEDEEMMGPNHVQ 300

Query: 301 SEEWADGCK 309
            EE   G +
Sbjct: 301 LEECETGSE 305

BLAST of Cp4.1LG05g03750 vs. NCBI nr
Match: XP_038875590.1 (heat stress transcription factor B-2b [Benincasa hispida] >XP_038875591.1 heat stress transcription factor B-2b [Benincasa hispida])

HSP 1 Score: 502 bits (1292), Expect = 6.80e-176
Identity = 268/315 (85.08%), Postives = 281/315 (89.21%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDP VDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCF+RGEK LLRDIQRRKVA+S+
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSL 120

Query: 121 AAPT---TPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTP 180
              T   TPA+V+VP V VAASPAV A VISP NS EEQVTSSNSSPM FQRGTSC TTP
Sbjct: 121 TTTTATATPAAVAVP-VAVAASPAVLAHVISPANSGEEQVTSSNSSPMTFQRGTSCTTTP 180

Query: 181 ELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQ-QLESVSVRDGKALDLLP 240
           ELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYAS H  QLESVSVRDGKAL+LLP
Sbjct: 181 ELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASDHNHQLESVSVRDGKALELLP 240

Query: 241 ARQMMDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEE--MV 300
           ARQ+M+DEGAVSDG QEVRLK+EE +A A   G TPKLFGVSIGVKR+RREE+DEE  MV
Sbjct: 241 ARQVMEDEGAVSDGAQEVRLKMEETMAAAV--GMTPKLFGVSIGVKRMRREEQDEEEEMV 300

Query: 301 GPNHVQSEEWADGCK 309
           G NHVQSEE   G +
Sbjct: 301 GQNHVQSEEGETGSE 312

BLAST of Cp4.1LG05g03750 vs. ExPASy TrEMBL
Match: A0A6J1H317 (heat stress transcription factor B-2b-like OS=Cucurbita moschata OX=3662 GN=LOC111459647 PE=3 SV=1)

HSP 1 Score: 586 bits (1511), Expect = 1.35e-209
Identity = 299/309 (96.76%), Postives = 302/309 (97.73%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVA+SV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVALSV 120

Query: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180
           AAP TPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV
Sbjct: 121 AAPATPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180

Query: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240
           RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM
Sbjct: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240

Query: 241 MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300
           MDDEGAVSDGIQEVRLKVEEAVA ATAEGRTPKLFGVSIGVKRVRREE+DEEMVGPNHVQ
Sbjct: 241 MDDEGAVSDGIQEVRLKVEEAVAVATAEGRTPKLFGVSIGVKRVRREEDDEEMVGPNHVQ 300

Query: 301 SEEWADGCK 309
           SEE   G +
Sbjct: 301 SEECETGSE 309

BLAST of Cp4.1LG05g03750 vs. ExPASy TrEMBL
Match: A0A6J1K1R0 (heat stress transcription factor B-2b-like OS=Cucurbita maxima OX=3661 GN=LOC111491622 PE=3 SV=1)

HSP 1 Score: 569 bits (1467), Expect = 5.84e-203
Identity = 294/309 (95.15%), Postives = 297/309 (96.12%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120

Query: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180
           AAP TPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQR TSCCTTPELV
Sbjct: 121 AAPATPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRVTSCCTTPELV 180

Query: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQM 240
           RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKAL+LLPARQM
Sbjct: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALELLPARQM 240

Query: 241 MDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMVGPNHVQ 300
           MDDEGAVSDGIQEVRLKVEEAVA    EGRTPKLFGVSIGVKRVRREEEDEEM+GPNHVQ
Sbjct: 241 MDDEGAVSDGIQEVRLKVEEAVA----EGRTPKLFGVSIGVKRVRREEEDEEMMGPNHVQ 300

Query: 301 SEEWADGCK 309
            EE   G +
Sbjct: 301 LEECETGSE 305

BLAST of Cp4.1LG05g03750 vs. ExPASy TrEMBL
Match: A0A1S3AU16 (heat stress transcription factor B-2b OS=Cucumis melo OX=3656 GN=LOC103482716 PE=3 SV=1)

HSP 1 Score: 499 bits (1284), Expect = 5.24e-175
Identity = 264/312 (84.62%), Postives = 277/312 (88.78%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDP VDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCF++GEK LLRDIQRRKVA+SV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVALSV 120

Query: 121 AAPTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPELV 180
              TT A+V+VP V VAASPAV A VISP NSAEEQVTSSNSSPM FQR TSC TTPELV
Sbjct: 121 TTTTTSAAVAVP-VPVAASPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPELV 180

Query: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG-HQQLESVSVRDGKALDLLPARQ 240
           RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG H   ES SVRDGKAL+LLPARQ
Sbjct: 181 RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHHHFESGSVRDGKALELLPARQ 240

Query: 241 MMDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRRE--EEDEEMVGPN 300
           +M+DEGAVSDG  EVRLK+ E +A A A G TPKLFGVSIGVKR+RRE  EE+EEMVG N
Sbjct: 241 VMEDEGAVSDGALEVRLKMGEKMAAAAAAGVTPKLFGVSIGVKRMRREVEEEEEEMVGQN 300

Query: 301 HVQSEEWADGCK 309
           HVQSEE   G +
Sbjct: 301 HVQSEEGETGSE 311

BLAST of Cp4.1LG05g03750 vs. ExPASy TrEMBL
Match: A0A0A0KNZ8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155480 PE=3 SV=1)

HSP 1 Score: 497 bits (1279), Expect = 3.24e-174
Identity = 266/314 (84.71%), Postives = 280/314 (89.17%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           MAPSPAEPIGDSG GDSQRSIPTPFLTKTYQLVDDP VDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MAPSPAEPIGDSGTGDSQRSIPTPFLTKTYQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCF++GEK LLRDIQRRKV +SV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRKGEKGLLRDIQRRKVVLSV 120

Query: 121 AAPTTP-ASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPEL 180
              TT  A+V+VP VTVA SPAV A VISP NSAEEQVTSSNSSPM FQR TSC TTPEL
Sbjct: 121 TTTTTTSAAVAVP-VTVATSPAVLAHVISPANSAEEQVTSSNSSPMAFQRSTSCTTTPEL 180

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG-HQQLESVSVRDGKALDLLPAR 240
           VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASG HQQLES SVRDGKAL+LLPAR
Sbjct: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGQHQQLESGSVRDGKALELLPAR 240

Query: 241 QMMDDEGAVSDGIQEVRLKVEEAV-ATATAEGRTPKLFGVSIGVKRVRRE--EEDEEMVG 300
           Q+M+DEGAVSDG  EVRLK+EE + A A A G TPKLFGVSIG+KR+RRE  EE+EEMVG
Sbjct: 241 QVMEDEGAVSDGAHEVRLKMEEKMTAAAAAVGMTPKLFGVSIGMKRMRREIEEEEEEMVG 300

Query: 301 PNHVQSEEWADGCK 309
            NHVQSEE   G +
Sbjct: 301 QNHVQSEEGETGSE 313

BLAST of Cp4.1LG05g03750 vs. ExPASy TrEMBL
Match: A0A6J1DU11 (heat stress transcription factor B-2b OS=Momordica charantia OX=3673 GN=LOC111024372 PE=3 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 1.27e-173
Identity = 268/315 (85.08%), Postives = 284/315 (90.16%), Query Frame = 0

Query: 1   MAPSPAEPIGDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFA 60
           M+PSPAEPIG+SG GDSQRSIPTPFLTKT+QLVDDP VDDLISWNEDGSTFIVWRPAEFA
Sbjct: 1   MSPSPAEPIGESGTGDSQRSIPTPFLTKTFQLVDDPAVDDLISWNEDGSTFIVWRPAEFA 60

Query: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSV 120
           RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCF+RGEK LLRDIQRRKVA+SV
Sbjct: 61  RDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFANDCFRRGEKGLLRDIQRRKVALSV 120

Query: 121 AA-PTTPASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEFQRGTSCCTTPEL 180
           A  P TPA++  P VTVAA+PAVA  VISP NS EEQVTSSNSSPM FQRGTSC TTPEL
Sbjct: 121 ATTPPTPAAMVSP-VTVAAAPAVAH-VISPANSGEEQVTSSNSSPMAFQRGTSCTTTPEL 180

Query: 181 VRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQ 240
           +RENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQ  ESVSVRDGKAL+L+PA Q
Sbjct: 181 LRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQS-ESVSVRDGKALELMPATQ 240

Query: 241 -MMDDEGAVSDGIQEVRLKVEEAV--ATATAEGRTPKLFGVSIGVKRVRREEEDEE--MV 300
            MM+DEGAVSDGIQE+RLKVEEA   A A AEG TPKLFGVSIGVKRVRREEE+EE  MV
Sbjct: 241 VMMEDEGAVSDGIQELRLKVEEATTAAAAAAEGVTPKLFGVSIGVKRVRREEEEEEEEMV 300

Query: 301 GPNHVQSEEWADGCK 309
           G NHVQSEE  +G +
Sbjct: 301 GQNHVQSEEGENGSE 312

BLAST of Cp4.1LG05g03750 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 330.5 bits (846), Expect = 1.7e-90
Identity = 184/296 (62.16%), Postives = 210/296 (70.95%), Query Frame = 0

Query: 10  GDSGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFK 69
           G  G GDSQRSIPTPFLTKTYQLV+DP  D+LISWNEDG+TFIVWRPAEFARDLLPKYFK
Sbjct: 45  GGGGGGDSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFK 104

Query: 70  HNNFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKV---AVSVAAPTTP 129
           HNNFSSFVRQLNTYGFRKVVPDRWEF+NDCFKRGEK LLRDIQRRK+   A++ AA    
Sbjct: 105 HNNFSSFVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAA 164

Query: 130 ASVSVPLVTVAASPAVAACVISPVNSAEEQVTSSNSSPM-------------EFQRGTSC 189
           A+V+   VTVAA P VA  ++SP NS EEQV SSNSSP                QR TSC
Sbjct: 165 AAVAASAVTVAAVPVVAH-IVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSC 224

Query: 190 CTTPELVRENERLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALD 249
            T PELV ENERLRK+N +L  E+T+LKGL  NI +LM N+  G +    + + +GK LD
Sbjct: 225 TTAPELVEENERLRKDNERLRKEMTKLKGLYANIYTLMANFTPGQEDCAHL-LPEGKPLD 284

Query: 250 LLPARQMMDDEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRREEE 290
           LLP RQ M +    S+        +E  +     E  TP+LFGVSIGVKR RREEE
Sbjct: 285 LLPERQEMSEAIMASE--------IETGIGLKLGEDLTPRLFGVSIGVKRARREEE 330

BLAST of Cp4.1LG05g03750 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 241.9 bits (616), Expect = 7.9e-64
Identity = 143/305 (46.89%), Postives = 189/305 (61.97%), Query Frame = 0

Query: 12  SGNGDSQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHN 71
           +G   SQRSIPTPFLTKT+ LV+D  +DD+ISWNEDGS+FIVW P +FA+DLLPK+FKHN
Sbjct: 11  TGESSSQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHN 70

Query: 72  NFSSFVRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSVAAPTTPASVSV 131
           NFSSFVRQLNTYGF+KVVPDRWEF+ND FKRGEK LLR+IQRRK+       TT  +V  
Sbjct: 71  NFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKIT------TTHQTVVA 130

Query: 132 PLVTVAASPAVAACVISPVNSAEE----QVTSSNSSPMEFQRGTSCCT---TPELVRENE 191
           P    ++       V+SP NS E+    QV SS+ S     +  +      + EL+ ENE
Sbjct: 131 P----SSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENE 190

Query: 192 RLRKENMQLSHELTQLKGLCNNILSLMTNYASGHQQLESVSV--RDGKALDLLPARQMMD 251
           +LR +N+QL+ ELTQ+K +C+NI SLM+NY        S S      + ++ LPA++  +
Sbjct: 191 KLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKRFSE 250

Query: 252 DEGAVSDGIQEVRLKVEEAVATATAEGRTPKLFGVSIGVKRVRRE---EEDEEMVGPNHV 305
                        +++EE       E  +P+LFGV IG+KR R E    +   +VG N  
Sbjct: 251 -------------MEIEE------EEEASPRLFGVPIGLKRTRSEGVQVKTTAVVGENSD 286

BLAST of Cp4.1LG05g03750 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 186.0 bits (471), Expect = 5.2e-47
Identity = 117/279 (41.94%), Postives = 163/279 (58.42%), Query Frame = 0

Query: 17  SQRSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSF 76
           +QRS+P PFL+KTYQLVDD   DD++SWNE+G+ F+VW+ AEFA+DLLP+YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSVAAPTTPASVSVPLVTV 136
           +RQLNTYGFRK VPD+WEFAND F+RG + LL DI+RRK   SV A T    V      V
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRK---SVIASTAGKCV------V 126

Query: 137 AASPAVAACVISPVNSAEEQVTSSNSSPMEFQR-GTSCCTTPELVRENERLRKENMQLSH 196
             SP+      S     ++  +SS SSP   +  G+      +L  ENE+L++EN  LS 
Sbjct: 127 VGSPSE-----SNSGGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSS 186

Query: 197 ELTQLKGLCNNILSLMTNYASGHQQLESVSVRDGKALDLLPARQMMDDEGAVSDGIQEVR 256
           EL   K   + +++ +T +     +     ++ GK    + + +  + EG    G     
Sbjct: 187 ELAAAKKQRDELVTFLTGHLKVRPEQIDKMIKGGK-FKPVESDEESECEGCDGGG----- 246

Query: 257 LKVEEAVATATAEGRTPKLFGVSIGVKRVRREEEDEEMV 295
              EE V      G   KLFGV +  +R +R+ +++  V
Sbjct: 247 -GAEEGV------GEGLKLFGVWLKGERKKRDRDEKNYV 258

BLAST of Cp4.1LG05g03750 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 178.3 bits (451), Expect = 1.1e-44
Identity = 99/203 (48.77%), Postives = 125/203 (61.58%), Query Frame = 0

Query: 19  RSIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSFVR 78
           +++P PFLTKTYQLVDDP  D ++SW +D +TF+VWRP EFARDLLP YFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRKVAVSVAAPTTP------ASVSVP 138
           QLNTYGFRK+VPDRWEFAN+ FKRGEK LL +I RRK +  +    +P      A   +P
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 LVTVAASPAVAACVISPVNSAEEQVTSSNSSPMEF-QRGTSCCTTPELVRENERLRKENM 198
               +  P     V +P         S  S P    Q+  +      L  +NERLR+ N 
Sbjct: 149 FSGGSFFPLPPPRVTTPEEDHYWCDDSPPSRPRVIPQQIDTAAQVTALSEDNERLRRSNT 208

Query: 199 QLSHELTQLKGLCNNILSLMTNY 215
            L  EL  +K L N+I+  + N+
Sbjct: 209 VLMSELAHMKKLYNDIIYFVQNH 231

BLAST of Cp4.1LG05g03750 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 153.3 bits (386), Expect = 3.7e-37
Identity = 67/96 (69.79%), Postives = 80/96 (83.33%), Query Frame = 0

Query: 20  SIPTPFLTKTYQLVDDPDVDDLISWNEDGSTFIVWRPAEFARDLLPKYFKHNNFSSFVRQ 79
           S+P PFL+KTY +V+DP  D ++SW+   ++FIVW P EF+RDLLPKYFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 80  LNTYGFRKVVPDRWEFANDCFKRGEKALLRDIQRRK 116
           LNTYGFRKV PDRWEFAN+ F RG+K LL+ I RRK
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRK 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0D32.4e-8962.16Heat stress transcription factor B-2b OS=Arabidopsis thaliana OX=3702 GN=HSFB2B ... [more]
Q652B01.4e-6852.65Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9C86.8e-6848.68Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9SCW41.1e-6246.89Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A ... [more]
P223355.7e-5143.51Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023521234.11.24e-21298.38heat stress transcription factor B-2b-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG6606116.13.39e-21097.41Heat stress transcription factor B-2b, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022958418.12.78e-20996.76heat stress transcription factor B-2b-like [Cucurbita moschata] >XP_022958419.1 ... [more]
XP_022996371.11.21e-20295.15heat stress transcription factor B-2b-like [Cucurbita maxima] >XP_022996372.1 he... [more]
XP_038875590.16.80e-17685.08heat stress transcription factor B-2b [Benincasa hispida] >XP_038875591.1 heat s... [more]
Match NameE-valueIdentityDescription
A0A6J1H3171.35e-20996.76heat stress transcription factor B-2b-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1K1R05.84e-20395.15heat stress transcription factor B-2b-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A1S3AU165.24e-17584.62heat stress transcription factor B-2b OS=Cucumis melo OX=3656 GN=LOC103482716 PE... [more]
A0A0A0KNZ83.24e-17484.71HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G155480 ... [more]
A0A6J1DU111.27e-17385.08heat stress transcription factor B-2b OS=Momordica charantia OX=3673 GN=LOC11102... [more]
Match NameE-valueIdentityDescription
AT4G11660.11.7e-9062.16winged-helix DNA-binding transcription factor family protein [more]
AT5G62020.17.9e-6446.89heat shock transcription factor B2A [more]
AT4G36990.15.2e-4741.94heat shock factor 4 [more]
AT1G46264.11.1e-4448.77heat shock transcription factor B4 [more]
AT4G17750.13.7e-3769.79heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..203
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR10015:SF169HEAT STRESS TRANSCRIPTION FACTOR B-2Bcoord: 12..301
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 25..48
score: 48.57
coord: 76..88
score: 87.03
coord: 63..75
score: 81.98
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 1.1E-58
score: 211.0
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 1.1E-32
score: 112.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..115
e-value: 4.5E-38
score: 131.9
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..301
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG05g03750.1Cp4.1LG05g03750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006012 galactose metabolic process
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0003978 UDP-glucose 4-epimerase activity
molecular_function GO:0043565 sequence-specific DNA binding