Cp4.1LG05g02280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG05g02280 vs. ExPASy Swiss-Prot
Match: Q9LZ20 (Cationic amino acid transporter 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT6 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 1.0e-139
Identity = 271/548 (49.45%), Postives = 365/548 (66.61%), Query Frame = 0

Query: 17  LRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGV 76
           LR Y++SLS TP RL +R ++  T   E+++VR  SG  M+R L+W+DLI LG+GGM+G 
Sbjct: 18  LRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGGMVGA 77

Query: 77  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEF 136
           GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+TFGEF
Sbjct: 78  GVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRITFGEF 137

Query: 137 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVA 196
             +F GAN++M+YV+SNAAV+RSFT YL  AFG    + WR  V GL KG+N +D  AV 
Sbjct: 138 PAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFG-ISTSKWRFVVSGLPKGFNEIDPVAVL 197

Query: 197 LILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVK------PGGLA 256
           ++L+IT+ +C ST+ESS +N+IMT FH+ F  F+I  G  KG + NL        P G  
Sbjct: 198 VVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPSGFF 257

Query: 257 PFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSL 316
           PFGA GV +GAA+VY SYIGYD+ ST+AEE++NP K +P+G+ GSV I + LYCLMA+S+
Sbjct: 258 PFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMAVSM 317

Query: 317 SLMVPYDQISEKAAFSIAFQ-RIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGR 376
           S+++PYD I  +A FS AF+   GW+W + ++G GAS GI+ SLLVAMLGQARY+C IGR
Sbjct: 318 SMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCVIGR 377

Query: 377 ARLVPFWLAKLY---------------------------IVIEMISIGTLMVFYLVANAT 436
           +R+VPFW AK++                           +++ ++SIGTL VFY+VANA 
Sbjct: 378 SRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVANAL 437

Query: 437 IYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSF-FSASTIFIIAL--- 496
           I+RRYV V    P   L FL   S +++ F+L WKL  +    +F   AS +  IA+   
Sbjct: 438 IFRRYVPVGPTKPWPTLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVAIAIVLS 497

Query: 497 FHYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYG 527
           F   VP     E W VP MPW   +SIFLN+FL+ +L   S+ RFG +S LI L Y+ YG
Sbjct: 498 FQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVLVYLFYG 557

BLAST of Cp4.1LG05g02280 vs. ExPASy Swiss-Prot
Match: Q9SQZ0 (Cationic amino acid transporter 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAT7 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 6.2e-137
Identity = 262/548 (47.81%), Postives = 359/548 (65.51%), Query Frame = 0

Query: 17  LRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGV 76
           LR Y++SLS TP R  +R ++  T   E+++VR  SG  M+R L+W+DLI LG+GGM+G 
Sbjct: 17  LRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGIGGMIGA 76

Query: 77  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEF 136
           GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+TFGEF
Sbjct: 77  GVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRITFGEF 136

Query: 137 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVA 196
             +  GAN+IM+YVLSNAAV+R FT YL  AFG    + WR  V GL  G+N +D  AV 
Sbjct: 137 PAFITGANLIMDYVLSNAAVSRGFTAYLGSAFG-ISTSEWRFIVSGLPNGFNEIDPIAVI 196

Query: 197 LILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVK------PGGLA 256
           ++L +T  +C+ST+ESS +N+++T  H+ F  F+I  G  KG   NL +      P G  
Sbjct: 197 VVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPENPSGFF 256

Query: 257 PFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSL 316
           PFG  GV +GAA+VY SYIGYD+ ST+AEE+++P K +P+GI GSV I   LYCLMA+S+
Sbjct: 257 PFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCLMAISM 316

Query: 317 SLMVPYDQISEKAAFSIAFQRI-GWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGR 376
           S+++PYD I  +A +S AF +  GW+W + ++G GAS GI+ SL+VAMLGQARY+C IGR
Sbjct: 317 SMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYMCVIGR 376

Query: 377 ARLVPFWLAKLY---------------------------IVIEMISIGTLMVFYLVANAT 436
           +R+VP W AK++                           +++ ++SIGTL VFY+VANA 
Sbjct: 377 SRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYMVANAV 436

Query: 437 IYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKL----NQQWWGLSFFSASTIFIIAL 496
           I+RRYV V    P   L FL   S ++I F+L W+L      +W+ L   + + I I+ +
Sbjct: 437 IFRRYVTVGYTEPWPTLSFLCLFSITSILFTLVWQLAPSGPPKWFILGASTVTAIAIVQI 496

Query: 497 FHYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYG 527
           FH  VP     E W VPLMPW   +SIFLN+FL+ +L   S+ RFG +S L+ L YV Y 
Sbjct: 497 FHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLVVLVYVFYS 556

BLAST of Cp4.1LG05g02280 vs. ExPASy Swiss-Prot
Match: Q84MA5 (Cationic amino acid transporter 1 OS=Arabidopsis thaliana OX=3702 GN=CAT1 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 4.2e-101
Identity = 204/547 (37.29%), Postives = 323/547 (59.05%), Query Frame = 0

Query: 19  HYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGVGV 78
           +Y+ +L +TP R   R++       E+N+++ RSG +MK+ L W+DL+  G+G ++G G+
Sbjct: 33  NYLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWDLMWFGIGAVIGSGI 92

Query: 79  FVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEFVG 138
           FV TG  A + +GPAV LSY+++G+SA+LS  CYTEF+V I  AGG+F+YLR+  G+F+ 
Sbjct: 93  FVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGGSFAYLRVELGDFMA 152

Query: 139 YFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVALI 198
           + A  NII+EYV+  AAVARS+T Y       K  + +R+ VH L + Y+ LD  AV + 
Sbjct: 153 FIAAGNIILEYVVGGAAVARSWTSYFATLLNHKPED-FRIIVHKLGEDYSHLDPIAVGVC 212

Query: 199 LLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPGGLAPFGAKGVL 258
            +I +     TK SS  N I ++ H++   F+I  G  K    N        P+G +GV 
Sbjct: 213 AIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNY---SDFTPYGVRGVF 272

Query: 259 DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMVPYDQ 318
             AA+++F+YIG+D+ ST+AEE +NP + +PIG+VGS+++T+  YCLMA++L LM PY Q
Sbjct: 273 KSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMAVTLCLMQPYQQ 332

Query: 319 ISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGRARLVPFWLA 378
           I   A FS+AF  +GW WA  ++  GA  G+   LLV  +GQARY+  I RA ++P WLA
Sbjct: 333 IDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHIARAHMMPPWLA 392

Query: 379 ---------------------------KLYIVIEMISIGTLMVFYLVANATIYRRYVM-- 438
                                      KL I+ +++S+ TL +F  VA A + RRY +  
Sbjct: 393 QVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSVSTLFIFMFVAVALLVRRYYVTG 452

Query: 439 -VSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFHYK--VPSH 498
             S    ++ L+FL  +  S+   ++ W L ++ W + +     I+ ++    K  VP  
Sbjct: 453 ETSTRDRNKFLVFLGLILASSTATAVYWALEEEGW-IGYCITVPIWFLSTVAMKFLVPQA 512

Query: 499 HSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVHSTYKAE 534
            + + W VPL+PW  + SI +N+FL+ ++   SF RF IW+ ++ ++YV++G+H+TY   
Sbjct: 513 RAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAIWTGILLIYYVLFGLHATYDTA 572

BLAST of Cp4.1LG05g02280 vs. ExPASy Swiss-Prot
Match: Q9SHH0 (Cationic amino acid transporter 8, vacuolar OS=Arabidopsis thaliana OX=3702 GN=CAT8 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.7e-89
Identity = 206/534 (38.58%), Postives = 307/534 (57.49%), Query Frame = 0

Query: 20  YIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGVGVF 79
           Y  +LS T  RL  R+L+  +   EL+  R+ S   M+R L W+DL+ L  G ++G GVF
Sbjct: 45  YKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWDLLWLSFGSVVGSGVF 104

Query: 80  VTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEFVGY 139
           V TG  A    GPAV LSY I+G+SALLS LCY EF V I  AGG+FSYLR+  G+F+ +
Sbjct: 105 VITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGGSFSYLRVELGDFIAF 164

Query: 140 FAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVALIL 199
            A  NI++E ++  A + RS++ YL  +  + +++ +R++V    KG+++LD  AVA++L
Sbjct: 165 IAAGNILLEAMVGAAGLGRSWSSYL-ASLVKNDSDYFRIKVDSFAKGFDLLDPVAVAVLL 224

Query: 200 LITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPGGLAPFGAKGVLD 259
           +        TK +S LNLI ++  V    FI+  G      +NLV      P+GAKGV+ 
Sbjct: 225 VANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP---FFPYGAKGVVQ 284

Query: 260 GAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMVPYDQI 319
            AA+VY+SY G+D  + +AEE + P++ +PIG+VGS+ + + +YCLMAL+L++MV Y +I
Sbjct: 285 SAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMALALTMMVKYTEI 344

Query: 320 SEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGRARLVPFWLA- 379
              AA+S+AF +IG KWA  L+G  A  G+  SLLV  LGQARY   I R+ ++P W A 
Sbjct: 345 DANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIARSHMIPPWFAL 404

Query: 380 --------------------------KLYIVIEMISIGTLMVFYLVANATIYRRYVMVSK 439
                                      L ++  + S  TL +F LVA A + RRY +   
Sbjct: 405 VHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAVALLVRRYYVKDV 464

Query: 440 HPPSRVLLFLVFL---SCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFHYKVPSHHSS 499
            P + +L FL FL     S+IG S  W    + W     +    FI  L    +P +   
Sbjct: 465 TPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVTGVIWFIGTLGLALLPKYRVP 524

Query: 500 EEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVHSTY 524
           + W VPL+PW  + SI +N+FL+ +L  ++F RF I + ++ L+Y+  G+H+TY
Sbjct: 525 KVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLYYLFVGLHATY 574

BLAST of Cp4.1LG05g02280 vs. ExPASy Swiss-Prot
Match: O64759 (Cationic amino acid transporter 5 OS=Arabidopsis thaliana OX=3702 GN=CAT5 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 7.7e-87
Identity = 194/540 (35.93%), Postives = 308/540 (57.04%), Query Frame = 0

Query: 20  YIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGVGVF 79
           Y  +LSQT  R K R+++    + E  +++++S  +MKR L W+DL+  G G ++G G+F
Sbjct: 28  YRAALSQTCSRFKNRLVSRSDDENERFELKKQSEHEMKRCLTWWDLVWFGFGSVIGAGIF 87

Query: 80  VTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEFVGY 139
           V TG  A    GPA+ LSY+++G+SA+LS  CYTEF+V I  AGG+F+YLR+  G+F  +
Sbjct: 88  VLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTEFAVEIPVAGGSFAYLRIELGDFAAF 147

Query: 140 FAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVALIL 199
               NI++E ++  AAVAR++T Y       +  NA R++   L  G+N+LD  AV +I 
Sbjct: 148 ITAGNILLESIVGTAAVARAWTSYFATLL-NRSPNALRIKT-DLSSGFNLLDPIAVVVIA 207

Query: 200 LITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPGGLAPFGAKGVLD 259
                   ST+++S LN I +  + +   F+I  G      +NL       PFG +GV  
Sbjct: 208 ASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFIHADTSNLTP---FLPFGPEGVFR 267

Query: 260 GAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMVPYDQI 319
            AA+VYF+Y G+DS +T+AEE +NP++ +PIG++GS+ I + +YCLMALSLS+M  Y  I
Sbjct: 268 AAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSIITVIYCLMALSLSMMQKYTDI 327

Query: 320 SEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGRARLVP----- 379
              AA+S+AFQ +G KW   L+  GA  G+   LLV  LGQARY+  I R  ++P     
Sbjct: 328 DPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALGQARYVTHIARTHMIPPIFAL 387

Query: 380 ----------------------FWLAKLYIVIEMISIGTLMVFYLVANATIYRRYVMVSK 439
                                  + + L ++  ++SI TL +F ++  A + RRY +   
Sbjct: 388 VHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSISTLFIFTMMPIALLVRRYYVRQD 447

Query: 440 HPP---SRVLLFLVFLSCSAIGFSLSWKLNQQ--WWG----LSFFSASTIFIIALFHYKV 499
            P     +++  L+F+  S++G S  W + ++  W G    + F+   T+ I+    + V
Sbjct: 448 TPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGYTVTVPFWFLGTLGIV----FFV 507

Query: 500 PSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVHSTY 524
           P   + + W VPL+PW   +SI  N+FLM +L  ++F RFG+ +  + L+Y + G+H+T+
Sbjct: 508 PQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGVCTLAMLLYYFLLGLHATF 558

BLAST of Cp4.1LG05g02280 vs. NCBI nr
Match: XP_023534059.1 (cationic amino acid transporter 6, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023534063.1 cationic amino acid transporter 6, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1048 bits (2710), Expect = 0.0
Identity = 547/574 (95.30%), Postives = 547/574 (95.30%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 487
           ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH
Sbjct: 421 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 480

Query: 488 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 547
           YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH
Sbjct: 481 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 540

Query: 548 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 554
           STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL
Sbjct: 541 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 574

BLAST of Cp4.1LG05g02280 vs. NCBI nr
Match: XP_022957739.1 (cationic amino acid transporter 6, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1046 bits (2704), Expect = 0.0
Identity = 546/574 (95.12%), Postives = 546/574 (95.12%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 487
           ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH
Sbjct: 421 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 480

Query: 488 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 547
           YKVPSHHSSEEW VPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH
Sbjct: 481 YKVPSHHSSEEWLVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 540

Query: 548 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 554
           STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL
Sbjct: 541 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 574

BLAST of Cp4.1LG05g02280 vs. NCBI nr
Match: KAG6606211.1 (Cationic amino acid transporter 6, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036158.1 Cationic amino acid transporter 6, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1045 bits (2701), Expect = 0.0
Identity = 546/574 (95.12%), Postives = 546/574 (95.12%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFII CGMYKGSANNLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIVCGMYKGSANNLVKPG 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 487
           ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH
Sbjct: 421 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 480

Query: 488 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 547
           YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH
Sbjct: 481 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 540

Query: 548 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 554
           STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL
Sbjct: 541 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 574

BLAST of Cp4.1LG05g02280 vs. NCBI nr
Match: XP_022995554.1 (cationic amino acid transporter 6, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 542/574 (94.43%), Postives = 545/574 (94.95%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MA AVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MATAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVH+SAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 487
           ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH
Sbjct: 421 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 480

Query: 488 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 547
           YKVPSHHS+EEWSVPLMPWPA ISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH
Sbjct: 481 YKVPSHHSTEEWSVPLMPWPATISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 540

Query: 548 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 554
           STYKAEEMEMNNRVGEVT+VNSTLQPNKLDIQVL
Sbjct: 541 STYKAEEMEMNNRVGEVTHVNSTLQPNKLDIQVL 574

BLAST of Cp4.1LG05g02280 vs. NCBI nr
Match: XP_038887589.1 (cationic amino acid transporter 6, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 963 bits (2490), Expect = 0.0
Identity = 507/578 (87.72%), Postives = 524/578 (90.66%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MA AV+S FLR Y++SLSQTPHRL+KRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVSSTFLRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVH+SAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGE E NAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLKGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAV LILL+TLCLCHSTKESS LNLIMTVFHVIFFGFIIGCGMYKGSA NLVKPG
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSILNLIMTVFHVIFFGFIIGCGMYKGSAKNLVKPG 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFG KGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLMVPY+QISEKAAFSIAFQR+GWKWA NLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYNQISEKAAFSIAFQRLGWKWAGNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPKTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWW-GLSFFSASTIFIIALF 487
           ATIYRRYVMVSKHPPSR+LLFL+ LSCSAIGFSLSWKLNQQWW GL FF ASTI IIA F
Sbjct: 421 ATIYRRYVMVSKHPPSRILLFLLLLSCSAIGFSLSWKLNQQWWPGLLFFGASTISIIAFF 480

Query: 488 HYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGV 547
           HYK PS  +SE WSVP MPWPAA SIFLNVFLMTTL+MLSFQRF IWSCLITLFYV+YGV
Sbjct: 481 HYKFPSQDTSEAWSVPYMPWPAATSIFLNVFLMTTLRMLSFQRFAIWSCLITLFYVVYGV 540

Query: 548 HSTYKAEE--MEMNNRVGEVTN-VNSTLQPNKLDIQVL 554
           HSTYKAEE  ME+NNRVGEVTN VNST+Q +KLDIQVL
Sbjct: 541 HSTYKAEEIIMEVNNRVGEVTNNVNSTVQQSKLDIQVL 578

BLAST of Cp4.1LG05g02280 vs. ExPASy TrEMBL
Match: A0A6J1H2V1 (cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111459193 PE=4 SV=1)

HSP 1 Score: 1046 bits (2704), Expect = 0.0
Identity = 546/574 (95.12%), Postives = 546/574 (95.12%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 487
           ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH
Sbjct: 421 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 480

Query: 488 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 547
           YKVPSHHSSEEW VPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH
Sbjct: 481 YKVPSHHSSEEWLVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 540

Query: 548 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 554
           STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL
Sbjct: 541 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 574

BLAST of Cp4.1LG05g02280 vs. ExPASy TrEMBL
Match: A0A6J1K670 (cationic amino acid transporter 6, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491053 PE=4 SV=1)

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 542/574 (94.43%), Postives = 545/574 (94.95%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MA AVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MATAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVH+SAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG
Sbjct: 181 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 487
           ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH
Sbjct: 421 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFH 480

Query: 488 YKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 547
           YKVPSHHS+EEWSVPLMPWPA ISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH
Sbjct: 481 YKVPSHHSTEEWSVPLMPWPATISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVH 540

Query: 548 STYKAEEMEMNNRVGEVTNVNSTLQPNKLDIQVL 554
           STYKAEEMEMNNRVGEVT+VNSTLQPNKLDIQVL
Sbjct: 541 STYKAEEMEMNNRVGEVTHVNSTLQPNKLDIQVL 574

BLAST of Cp4.1LG05g02280 vs. ExPASy TrEMBL
Match: A0A5A7T4W3 (Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00360 PE=4 SV=1)

HSP 1 Score: 962 bits (2486), Expect = 0.0
Identity = 505/583 (86.62%), Postives = 524/583 (89.88%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MA AV S FLRHY++SLSQTPHRL+KRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MATAVFSTFLRHYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVH+SAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGE E NAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAV LILL+TLCLCHSTKESSTLNLIMT+FHVIFFGFIIGCGMYKGSA NLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFG KGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLM+PY+QISEKAAFS+AFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQISEKAAFSMAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTL+VFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLIVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWW-GLSFFSASTIFIIALF 487
           ATIYRRY MVSKHPPSR+LLFL+ LSCSAIGFSLSWKLNQQWW GL FF  STIFII  F
Sbjct: 421 ATIYRRYAMVSKHPPSRILLFLLLLSCSAIGFSLSWKLNQQWWPGLLFFGVSTIFIITFF 480

Query: 488 HYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGV 547
           HYK PSH+SS+ WSVP MPWPAA SIFLNVFLMTTL+MLSFQRF IWSCLITLFYV+YGV
Sbjct: 481 HYKFPSHNSSDAWSVPYMPWPAATSIFLNVFLMTTLRMLSFQRFAIWSCLITLFYVVYGV 540

Query: 548 HSTYKAEE--MEMNNRVGEVTN------VNSTLQPNKLDIQVL 554
           HSTYKAEE  ME+NNRVGEVTN      VNST+Q +KLDIQVL
Sbjct: 541 HSTYKAEEIIMEVNNRVGEVTNNNNNNNVNSTIQQSKLDIQVL 583

BLAST of Cp4.1LG05g02280 vs. ExPASy TrEMBL
Match: A0A0A0LFB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G912900 PE=4 SV=1)

HSP 1 Score: 956 bits (2470), Expect = 0.0
Identity = 503/586 (85.84%), Postives = 523/586 (89.25%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MA AV+S FLR Y++SLSQTPHRL+KRMLATWTPDQELNQVRQRSGADMKRKLKWFDL+A
Sbjct: 1   MATAVSSTFLRRYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLVA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVH+SAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGE E NAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAV LILL+TLCLCHSTKESSTLNLIMT+FHVIFFGFIIGCGMYKGSA NLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           G+APFG KGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GVAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLM+PY+QISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVP WLAK                           LYIVIEMISIGTLMVFYLVAN
Sbjct: 361 GRARLVPSWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLMVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWW-GLSFFSASTIFIIALF 487
           ATIYRRY MVSKHPPSR+LLFL+ LSCSAIGFSLSWKLNQQWW GL FFS STIFII  F
Sbjct: 421 ATIYRRYAMVSKHPPSRILLFLLLLSCSAIGFSLSWKLNQQWWPGLLFFSVSTIFIITFF 480

Query: 488 HYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGV 547
           HYK PSH+SS+ WSVP MPWPAA SIFLNVFLMTTL+MLSFQRF IWSCLITLFYV+YGV
Sbjct: 481 HYKFPSHNSSDAWSVPYMPWPAATSIFLNVFLMTTLRMLSFQRFAIWSCLITLFYVVYGV 540

Query: 548 HSTYKAEEMEM---NNRVGEVTN--------VNSTLQPNKLDIQVL 554
           HSTYKAEE+ M   NNRVGEVTN        VNST+Q +KLDIQVL
Sbjct: 541 HSTYKAEEIIMEVNNNRVGEVTNNNNNNNNNVNSTIQQSKLDIQVL 586

BLAST of Cp4.1LG05g02280 vs. ExPASy TrEMBL
Match: A0A5D3E636 (Cationic amino acid transporter 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003830 PE=4 SV=1)

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 502/583 (86.11%), Postives = 521/583 (89.37%), Query Frame = 0

Query: 8   MAAAVNSAFLRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 67
           MA AV S FLRHY++SLSQTPHRL+KRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA
Sbjct: 1   MATAVFSTFLRHYLYSLSQTPHRLRKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIA 60

Query: 68  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFS 127
           LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVH+SAAGGAFS
Sbjct: 61  LGVGGMLGVGVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHVSAAGGAFS 120

Query: 128 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGY 187
           YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGE E NAWRVEVHGLL GY
Sbjct: 121 YLRLTFGEFVGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGESEPNAWRVEVHGLLNGY 180

Query: 188 NMLDFPAVALILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPG 247
           NMLDFPAV LILL+TLCLCHSTKESSTLNLIMT+FHVIFFGFIIGCGMYKGSA NLVKP 
Sbjct: 181 NMLDFPAVGLILLLTLCLCHSTKESSTLNLIMTIFHVIFFGFIIGCGMYKGSAKNLVKPD 240

Query: 248 GLAPFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 307
           GLAPFG KGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA
Sbjct: 241 GLAPFGVKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMA 300

Query: 308 LSLSLMVPYDQISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 367
           LSLSLM+PY+Q   KAAFS+AFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI
Sbjct: 301 LSLSLMLPYNQ---KAAFSMAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAI 360

Query: 368 GRARLVPFWLAK---------------------------LYIVIEMISIGTLMVFYLVAN 427
           GRARLVPFWLAK                           LYIVIEMISIGTL+VFYLVAN
Sbjct: 361 GRARLVPFWLAKVHPSTGTPLNATLFLGLCTASIALFTELYIVIEMISIGTLIVFYLVAN 420

Query: 428 ATIYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWW-GLSFFSASTIFIIALF 487
           ATIYRRY MVSKHPPSR+LLFL+ LSCSAIGFSLSWKLNQQWW GL FF  STIFII  F
Sbjct: 421 ATIYRRYAMVSKHPPSRILLFLLLLSCSAIGFSLSWKLNQQWWPGLLFFGVSTIFIITFF 480

Query: 488 HYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGV 547
           HYK PSH+SS+ WSVP MPWPAA SIFLNVFLMTTL+MLSFQRF IWSCLITLFYV+YGV
Sbjct: 481 HYKFPSHNSSDAWSVPYMPWPAATSIFLNVFLMTTLRMLSFQRFAIWSCLITLFYVVYGV 540

Query: 548 HSTYKAEE--MEMNNRVGEVTN------VNSTLQPNKLDIQVL 554
           HSTYKAEE  ME+NNRVGEVTN      VNST+Q +KLDIQVL
Sbjct: 541 HSTYKAEEIIMEVNNRVGEVTNNNNNNNVNSTIQQSKLDIQVL 580

BLAST of Cp4.1LG05g02280 vs. TAIR 10
Match: AT5G04770.1 (cationic amino acid transporter 6 )

HSP 1 Score: 498.4 bits (1282), Expect = 7.3e-141
Identity = 271/548 (49.45%), Postives = 365/548 (66.61%), Query Frame = 0

Query: 17  LRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGV 76
           LR Y++SLS TP RL +R ++  T   E+++VR  SG  M+R L+W+DLI LG+GGM+G 
Sbjct: 18  LRVYLNSLSATPSRLSRRAISVSTSSDEMSRVRAVSGEQMRRTLRWYDLIGLGIGGMVGA 77

Query: 77  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEF 136
           GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+TFGEF
Sbjct: 78  GVFVTTGRASRLDAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRITFGEF 137

Query: 137 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVA 196
             +F GAN++M+YV+SNAAV+RSFT YL  AFG    + WR  V GL KG+N +D  AV 
Sbjct: 138 PAFFTGANLVMDYVMSNAAVSRSFTAYLGTAFG-ISTSKWRFVVSGLPKGFNEIDPVAVL 197

Query: 197 LILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVK------PGGLA 256
           ++L+IT+ +C ST+ESS +N+IMT FH+ F  F+I  G  KG + NL        P G  
Sbjct: 198 VVLVITVIICCSTRESSKVNMIMTAFHIAFIFFVIVMGFIKGDSKNLSSPANPEHPSGFF 257

Query: 257 PFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSL 316
           PFGA GV +GAA+VY SYIGYD+ ST+AEE++NP K +P+G+ GSV I + LYCLMA+S+
Sbjct: 258 PFGAAGVFNGAAMVYLSYIGYDAVSTMAEEVENPVKDIPVGVSGSVAIVTVLYCLMAVSM 317

Query: 317 SLMVPYDQISEKAAFSIAFQ-RIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGR 376
           S+++PYD I  +A FS AF+   GW+W + ++G GAS GI+ SLLVAMLGQARY+C IGR
Sbjct: 318 SMLLPYDLIDPEAPFSAAFRGSNGWEWVTKVVGIGASFGILTSLLVAMLGQARYMCVIGR 377

Query: 377 ARLVPFWLAKLY---------------------------IVIEMISIGTLMVFYLVANAT 436
           +R+VPFW AK++                           +++ ++SIGTL VFY+VANA 
Sbjct: 378 SRVVPFWFAKIHPKTSTPVNASTFLGIFTAALALFTDLNVLLNLVSIGTLFVFYMVANAL 437

Query: 437 IYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSF-FSASTIFIIAL--- 496
           I+RRYV V    P   L FL   S +++ F+L WKL  +    +F   AS +  IA+   
Sbjct: 438 IFRRYVPVGPTKPWPTLCFLTLFSITSLVFTLIWKLVPEGKPKAFMLGASAVVAIAIVLS 497

Query: 497 FHYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYG 527
           F   VP     E W VP MPW   +SIFLN+FL+ +L   S+ RFG +S LI L Y+ YG
Sbjct: 498 FQCVVPQARKPELWGVPFMPWTPCVSIFLNIFLLGSLDAPSYVRFGFFSGLIVLVYLFYG 557

BLAST of Cp4.1LG05g02280 vs. TAIR 10
Match: AT3G10600.1 (cationic amino acid transporter 7 )

HSP 1 Score: 489.2 bits (1258), Expect = 4.4e-138
Identity = 262/548 (47.81%), Postives = 359/548 (65.51%), Query Frame = 0

Query: 17  LRHYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGV 76
           LR Y++SLS TP R  +R ++  T   E+++VR  SG  M+R L+W+DLI LG+GGM+G 
Sbjct: 17  LRVYLNSLSDTPSRFSRRAVSVSTSYDEMSRVRAVSGEQMRRTLRWYDLIGLGIGGMIGA 76

Query: 77  GVFVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEF 136
           GVFVTTG  +    GP++ +SY IAG+ ALLS+ CYTEF+VH+  AGGAFSY+R+TFGEF
Sbjct: 77  GVFVTTGRASRLYAGPSIVVSYAIAGLCALLSAFCYTEFAVHLPVAGGAFSYIRITFGEF 136

Query: 137 VGYFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVA 196
             +  GAN+IM+YVLSNAAV+R FT YL  AFG    + WR  V GL  G+N +D  AV 
Sbjct: 137 PAFITGANLIMDYVLSNAAVSRGFTAYLGSAFG-ISTSEWRFIVSGLPNGFNEIDPIAVI 196

Query: 197 LILLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVK------PGGLA 256
           ++L +T  +C+ST+ESS +N+++T  H+ F  F+I  G  KG   NL +      P G  
Sbjct: 197 VVLAVTFVICYSTRESSKVNMVLTALHIAFIVFVIVMGFSKGDVKNLTRPDNPENPSGFF 256

Query: 257 PFGAKGVLDGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSL 316
           PFG  GV +GAA+VY SYIGYD+ ST+AEE+++P K +P+GI GSV I   LYCLMA+S+
Sbjct: 257 PFGVSGVFNGAAMVYLSYIGYDAVSTMAEEVKDPVKDIPMGISGSVAIVIVLYCLMAISM 316

Query: 317 SLMVPYDQISEKAAFSIAFQRI-GWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGR 376
           S+++PYD I  +A +S AF +  GW+W + ++G GAS GI+ SL+VAMLGQARY+C IGR
Sbjct: 317 SMLLPYDLIDAEAPYSAAFSKSEGWEWVTRVVGIGASFGILTSLIVAMLGQARYMCVIGR 376

Query: 377 ARLVPFWLAKLY---------------------------IVIEMISIGTLMVFYLVANAT 436
           +R+VP W AK++                           +++ ++SIGTL VFY+VANA 
Sbjct: 377 SRVVPIWFAKVHPKTSTPVNASAFLGIFTAVLALFTDLNVLLNLVSIGTLFVFYMVANAV 436

Query: 437 IYRRYVMVSKHPPSRVLLFLVFLSCSAIGFSLSWKL----NQQWWGLSFFSASTIFIIAL 496
           I+RRYV V    P   L FL   S ++I F+L W+L      +W+ L   + + I I+ +
Sbjct: 437 IFRRYVTVGYTEPWPTLSFLCLFSITSILFTLVWQLAPSGPPKWFILGASTVTAIAIVQI 496

Query: 497 FHYKVPSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYG 527
           FH  VP     E W VPLMPW   +SIFLN+FL+ +L   S+ RFG +S L+ L YV Y 
Sbjct: 497 FHCVVPQARIPEFWGVPLMPWTPCVSIFLNIFLLGSLDAPSYIRFGFFSGLVVLVYVFYS 556

BLAST of Cp4.1LG05g02280 vs. TAIR 10
Match: AT4G21120.1 (amino acid transporter 1 )

HSP 1 Score: 370.2 bits (949), Expect = 3.0e-102
Identity = 204/547 (37.29%), Postives = 323/547 (59.05%), Query Frame = 0

Query: 19  HYIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGVGV 78
           +Y+ +L +TP R   R++       E+N+++ RSG +MK+ L W+DL+  G+G ++G G+
Sbjct: 33  NYLKALKETPSRFMDRIMTRSLDSDEINEMKARSGHEMKKTLTWWDLMWFGIGAVIGSGI 92

Query: 79  FVTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEFVG 138
           FV TG  A + +GPAV LSY+++G+SA+LS  CYTEF+V I  AGG+F+YLR+  G+F+ 
Sbjct: 93  FVLTGLEARNHSGPAVVLSYVVSGVSAMLSVFCYTEFAVEIPVAGGSFAYLRVELGDFMA 152

Query: 139 YFAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVALI 198
           + A  NII+EYV+  AAVARS+T Y       K  + +R+ VH L + Y+ LD  AV + 
Sbjct: 153 FIAAGNIILEYVVGGAAVARSWTSYFATLLNHKPED-FRIIVHKLGEDYSHLDPIAVGVC 212

Query: 199 LLITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPGGLAPFGAKGVL 258
            +I +     TK SS  N I ++ H++   F+I  G  K    N        P+G +GV 
Sbjct: 213 AIICVLAVVGTKGSSRFNYIASIIHMVVILFVIIAGFTKADVKNY---SDFTPYGVRGVF 272

Query: 259 DGAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMVPYDQ 318
             AA+++F+YIG+D+ ST+AEE +NP + +PIG+VGS+++T+  YCLMA++L LM PY Q
Sbjct: 273 KSAAVLFFAYIGFDAVSTMAEETKNPGRDIPIGLVGSMVVTTVCYCLMAVTLCLMQPYQQ 332

Query: 319 ISEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGRARLVPFWLA 378
           I   A FS+AF  +GW WA  ++  GA  G+   LLV  +GQARY+  I RA ++P WLA
Sbjct: 333 IDPDAPFSVAFSAVGWDWAKYIVAFGALKGMTTVLLVGAIGQARYMTHIARAHMMPPWLA 392

Query: 379 ---------------------------KLYIVIEMISIGTLMVFYLVANATIYRRYVM-- 438
                                      KL I+ +++S+ TL +F  VA A + RRY +  
Sbjct: 393 QVNAKTGTPINATVVMLAATALIAFFTKLKILADLLSVSTLFIFMFVAVALLVRRYYVTG 452

Query: 439 -VSKHPPSRVLLFLVFLSCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFHYK--VPSH 498
             S    ++ L+FL  +  S+   ++ W L ++ W + +     I+ ++    K  VP  
Sbjct: 453 ETSTRDRNKFLVFLGLILASSTATAVYWALEEEGW-IGYCITVPIWFLSTVAMKFLVPQA 512

Query: 499 HSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVHSTYKAE 534
            + + W VPL+PW  + SI +N+FL+ ++   SF RF IW+ ++ ++YV++G+H+TY   
Sbjct: 513 RAPKIWGVPLVPWLPSASIAINIFLLGSIDTKSFVRFAIWTGILLIYYVLFGLHATYDTA 572

BLAST of Cp4.1LG05g02280 vs. TAIR 10
Match: AT1G17120.1 (cationic amino acid transporter 8 )

HSP 1 Score: 331.6 bits (849), Expect = 1.2e-90
Identity = 206/534 (38.58%), Postives = 307/534 (57.49%), Query Frame = 0

Query: 20  YIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGVGVF 79
           Y  +LS T  RL  R+L+  +   EL+  R+ S   M+R L W+DL+ L  G ++G GVF
Sbjct: 45  YKSALSATCPRLADRLLSRSSDAYELDAARRESENPMRRCLTWWDLLWLSFGSVVGSGVF 104

Query: 80  VTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEFVGY 139
           V TG  A    GPAV LSY I+G+SALLS LCY EF V I  AGG+FSYLR+  G+F+ +
Sbjct: 105 VITGQEARVGAGPAVVLSYAISGVSALLSVLCYAEFGVEIPVAGGSFSYLRVELGDFIAF 164

Query: 140 FAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVALIL 199
            A  NI++E ++  A + RS++ YL  +  + +++ +R++V    KG+++LD  AVA++L
Sbjct: 165 IAAGNILLEAMVGAAGLGRSWSSYL-ASLVKNDSDYFRIKVDSFAKGFDLLDPVAVAVLL 224

Query: 200 LITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPGGLAPFGAKGVLD 259
           +        TK +S LNLI ++  V    FI+  G      +NLV      P+GAKGV+ 
Sbjct: 225 VANGIAMTGTKRTSWLNLITSMVTVCIIVFIVVVGFTHSKTSNLVP---FFPYGAKGVVQ 284

Query: 260 GAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMVPYDQI 319
            AA+VY+SY G+D  + +AEE + P++ +PIG+VGS+ + + +YCLMAL+L++MV Y +I
Sbjct: 285 SAAVVYWSYTGFDMVANMAEETEKPSRDIPIGLVGSMSMITVVYCLMALALTMMVKYTEI 344

Query: 320 SEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGRARLVPFWLA- 379
              AA+S+AF +IG KWA  L+G  A  G+  SLLV  LGQARY   I R+ ++P W A 
Sbjct: 345 DANAAYSVAFAQIGMKWAKYLVGICALKGMTTSLLVGSLGQARYTTQIARSHMIPPWFAL 404

Query: 380 --------------------------KLYIVIEMISIGTLMVFYLVANATIYRRYVMVSK 439
                                      L ++  + S  TL +F LVA A + RRY +   
Sbjct: 405 VHPKTGTPIYATLLVTILSSIISFFTSLEVLSSVFSFATLFIFMLVAVALLVRRYYVKDV 464

Query: 440 HPPSRVLLFLVFL---SCSAIGFSLSWKLNQQWWGLSFFSASTIFIIALFHYKVPSHHSS 499
            P + +L FL FL     S+IG S  W    + W     +    FI  L    +P +   
Sbjct: 465 TPEAGLLKFLGFLFLIIASSIGVSALWNSGVKGWIAYTVTGVIWFIGTLGLALLPKYRVP 524

Query: 500 EEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVHSTY 524
           + W VPL+PW  + SI +N+FL+ +L  ++F RF I + ++ L+Y+  G+H+TY
Sbjct: 525 KVWGVPLVPWLPSFSIAMNLFLIGSLGYVAFLRFIICTMVMLLYYLFVGLHATY 574

BLAST of Cp4.1LG05g02280 vs. TAIR 10
Match: AT2G34960.1 (cationic amino acid transporter 5 )

HSP 1 Score: 322.8 bits (826), Expect = 5.5e-88
Identity = 194/540 (35.93%), Postives = 308/540 (57.04%), Query Frame = 0

Query: 20  YIHSLSQTPHRLKKRMLATWTPDQELNQVRQRSGADMKRKLKWFDLIALGVGGMLGVGVF 79
           Y  +LSQT  R K R+++    + E  +++++S  +MKR L W+DL+  G G ++G G+F
Sbjct: 28  YRAALSQTCSRFKNRLVSRSDDENERFELKKQSEHEMKRCLTWWDLVWFGFGSVIGAGIF 87

Query: 80  VTTGPVALHVTGPAVFLSYIIAGISALLSSLCYTEFSVHISAAGGAFSYLRLTFGEFVGY 139
           V TG  A    GPA+ LSY+++G+SA+LS  CYTEF+V I  AGG+F+YLR+  G+F  +
Sbjct: 88  VLTGQEAHEQAGPAIVLSYVVSGLSAMLSVFCYTEFAVEIPVAGGSFAYLRIELGDFAAF 147

Query: 140 FAGANIIMEYVLSNAAVARSFTEYLCVAFGEKEANAWRVEVHGLLKGYNMLDFPAVALIL 199
               NI++E ++  AAVAR++T Y       +  NA R++   L  G+N+LD  AV +I 
Sbjct: 148 ITAGNILLESIVGTAAVARAWTSYFATLL-NRSPNALRIKT-DLSSGFNLLDPIAVVVIA 207

Query: 200 LITLCLCHSTKESSTLNLIMTVFHVIFFGFIIGCGMYKGSANNLVKPGGLAPFGAKGVLD 259
                   ST+++S LN I +  + +   F+I  G      +NL       PFG +GV  
Sbjct: 208 ASATIASISTRKTSLLNWIASAINTLVIFFVIIAGFIHADTSNLTP---FLPFGPEGVFR 267

Query: 260 GAAIVYFSYIGYDSASTLAEEIQNPTKSLPIGIVGSVIITSALYCLMALSLSLMVPYDQI 319
            AA+VYF+Y G+DS +T+AEE +NP++ +PIG++GS+ I + +YCLMALSLS+M  Y  I
Sbjct: 268 AAAVVYFAYGGFDSIATMAEETKNPSRDIPIGLLGSMSIITVIYCLMALSLSMMQKYTDI 327

Query: 320 SEKAAFSIAFQRIGWKWASNLIGGGASLGIVASLLVAMLGQARYLCAIGRARLVP----- 379
              AA+S+AFQ +G KW   L+  GA  G+   LLV  LGQARY+  I R  ++P     
Sbjct: 328 DPNAAYSVAFQSVGMKWGKYLVALGALKGMTTVLLVGALGQARYVTHIARTHMIPPIFAL 387

Query: 380 ----------------------FWLAKLYIVIEMISIGTLMVFYLVANATIYRRYVMVSK 439
                                  + + L ++  ++SI TL +F ++  A + RRY +   
Sbjct: 388 VHPKTGTPINANLLVAIPSALIAFFSGLDVLASLLSISTLFIFTMMPIALLVRRYYVRQD 447

Query: 440 HPP---SRVLLFLVFLSCSAIGFSLSWKLNQQ--WWG----LSFFSASTIFIIALFHYKV 499
            P     +++  L+F+  S++G S  W + ++  W G    + F+   T+ I+    + V
Sbjct: 448 TPRVHLIKLITCLLFVVVSSMGTSAYWGMQRKGSWIGYTVTVPFWFLGTLGIV----FFV 507

Query: 500 PSHHSSEEWSVPLMPWPAAISIFLNVFLMTTLKMLSFQRFGIWSCLITLFYVMYGVHSTY 524
           P   + + W VPL+PW   +SI  N+FLM +L  ++F RFG+ +  + L+Y + G+H+T+
Sbjct: 508 PQQRTPKVWGVPLVPWLPCLSIATNIFLMGSLGAMAFVRFGVCTLAMLLYYFLLGLHATF 558