Cp4.1LG04g15230 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g15230
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type domain-containing protein
LocationCp4.1LG04: 12041764 .. 12045735 (+)
RNA-Seq ExpressionCp4.1LG04g15230
SyntenyCp4.1LG04g15230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGGCCGGGAATTTAGGAAAACGGAGGGATATTTTGGTAAATATTTAAATCAAATCTCGGCCCTCTGTTTTTCCTAGTCTGTGGAAAAGAGCGAGCGAAGCAGAGTAGTTTCCGTTATCCATTATCTCTCTTTCTCCTTCCAAGGTCGTCTTCTCAATTATCCTCGCAAATTAGGGTTTTTCGCGAACTTCTTCCCTTCATTTCGATTTCAACTCTTCAATCATGGTTACTGATTCGATTACCAATGCTTCAGTTTCTTTGGCTCCGAACGCTGGGGATTTGCCCTTGCCCAAGAAGAAGAGGGTGAGGACTCTCTCTCTCTTTATCTTCCGACGTAAATCTTCTAATTTTTTTCTTTTAATTTTAATGCTTTTTTCTTTTCTTTTTTCTTTTGTTGATGTTCGACGGTTGGTTTTCTTTTTCTGATATGTCGTAACTGTTTTGGTGGGGGGTTTCAGGTTAGGTATTCCAAATTGAAGCAGTCCAAGCTTGATGTTCGTCGGGAACAATGGCTTTCTCGAGGTATAACTTATAATTATGTTTTCTTTTGTTTGTTAATTGCATATATGTATGGAACGGGATTTATGCTTGTGAGAATTTGTGTTTAATCGATTTGGGTTCACGAGTTATAGGTGCTGTAAAGAACAAAAAATGGAACGAAGACAATCGTCTTGACTCAGTGGTCGTTAAAACGAGGAAAGATGATGATCTTTCCTCTAATAAAATAAATTCGAGGGAAATAGGAGAACAGAACAACAGATCGGTCCACCACCACAGCGATTTTTCAGAGTCGCCGTCTAATAGTCCCCCTAGTCTTGGGAGTAGTATCCTGTGTGGTAATGATTCGGTTCCTCACTTTACTGGGAGTAGCAGTAGTAGTAGTTGCCGAAGCAGCTCTAGTGGTTGTCAGTCGGGGAGTCTAACGGAGGAGGATGAAGGAGATGATGACTGCTTGGATGATTGGGAGGCTATTGCCGATGCCCTAGCCGCCACTGACAAGCAGCATGATCAATGTTCAGAGTCTTCCCCTAGCGGTAATATTGTTTCTCAATTGGATTCTTGCGGAGACAGCAGAAATGAGTTGGGCGTAGGAGATGGTGACTCGAGTATTGAACTCGGGAAAATTGTGCAGAGGGCTTCGATGAATTGCCGAGCATGGAGGCCTGATGATGCCTTTCGTCCTCAGAGTCTGCCTACGTTATCAAAGCAGCTTAGTTTACCGAATACAGACCGGCATTTTGGATATGGAGGCATCTCTTGGGCCTGTGGTGGTGTTATGCCTGTGCCTACATCTTGTCCAATTTGCTTTGAAGACTTAGACTTTACAGACTCGAGTTTCTTACCTTGTTTCTGTGGATTTCGGCTTTGCCTTTTCTGTCATAAAAGGATTCTTGAAGAGGATGGGCGCTGTCCTGGTTGCAGGAAGCCATATGATCGTGACCATGGTGACAATGAAACAAATGTAGATGGTGGTAGCCCAATGTTCCTGTTGGCACGTTCTTATAGCATGATTTCAAGGTCTTAGCTAGTGTCCAGTTTCTGTGAGTTTNACCTTTTTTTTTTTTTTTTTGTTCGTACGTCTCTTGGGTATTATGGTGGTCTGCTCTGCTTTCATAGCAATAGATGTGGCATTTGGGTGTTGAAGTATGGTTATGAGATTGAGAACCTTTAAACTGATTTGCTTCGTACATGAGTGTGGTTCTTATCTTATGATACATTTGCCAATGTAGAGGACTTATTTCGTACTTGATATGTGCATAGATGAGGTGGTATAGAAAATACATGGAATAATGATACAACATTTATTTTTGTCCGCATCTTATTTATTTGTATTTGCATATATTTCATAGAAATGCCTGAAACTGTTCTAGTTAGTACTTTCCTCCTTATTCTTGCTGGTTTTGTGATACTTAGTGTCGTGATTGACTACTCATATATGGTTTTGTAGCATGTGGCAAGACTCCCAGGAGAATATACACGCTAAAATGAAATGTGGAAATATTTGTAATAACTTCTATGTCACACAACTAATTGTTCAATGCTTTTGATGTACATAGCCAGCACCAGCAGTTGAATGTCTGAGATCAGTCAAAGGTAAAAGCTTAACTTGTTTTGCTTTAGAGTTTAGACATGTTGGTTTACTATTGTTTTAATGACTGTTTTAGGATCTGAGGAGTGAACACTGAACGTGGATTGTTATAGAAGAATCTTTGGTTTGATGCACTTTAACATATCTTCATTGGGTTATAGTTCACTTTTTTTTTTTCTTCATAAACTACTCTTAGCGCTGATGTTTAATGATTGGGACCCTTAATTGTAACTTTTTGGTTCTAACCTGTTCTATTTTGACAGCGTTCTTTTTTATGTATGTTGATTCTTCTTAATAAGAGTGGGTTCCCTTTTTTCATTCATTTCAAAATGAAAAAAGAACATTTCTTCAAAAATTTATGTCTAAGAAATTATTAGCTAAACTTGAAAGACAGTGATGCAGATGACAGTTTGAATTAGATGGATTGAAATGCCACTTTGAAGCTTCTTGATGAATGACCCATTTTATTTCCCTAATACTAAGTTTATGGTTGTGTTTTACTGAATATAAAGGGTCTTAGCGTCCGTTCTATACTGGCAGTGGCGGGACATAGTTGCTGGAAGGTAGATTCTGTAATTGGTTGATTTTTCCACAGAAATAAATTTCACCATGTAAGCTTGTATATCATGTATTCATTAGGGCATTGGGTAGATCTGCCTTTGATTGAGGTCCCATTATCTGTTTAGCTTACGATGGATTGAATAATGTGATTGCTGAATAACTGCAGCTATGGGCTTTTCTAATTCTTTTGAACGAGAACAAGGGTGCTGATCTCAACTTATCTACAATTCTCTATGTATATTGGGCTCTCTGTCACATAGAGGTAATTATGAAGGATAATCCCATGGCATGTAGGTATGAGCCACTTCTATACTGGGTATTCATTTGCTGTTGATATTGACTTAGCTGGTGCAAGTTATTAAGAAGATATTGATTGTTGATGGGTGGTCTTTTTAGTTAAACTATAACAATTGCTGCGAAAGAATTGAAAGTGGATTCTGAAGAATAAATTATCTGATAGTCTAATTTTCATTAGGCTATGCCTTACGCTGGATCATATATCCTTGTTTATGCTGAGTTCTAGAAGACTAACCGACACTTGTCTCCAAGAATTTAAGTACTTAGTAAGATCAGACTACTCCCAGTTCATAGCATCGTCTCTTTTTTCCATCCTCTGAATATCATCCATAAGACAAGACTGTGATCTGTGTTGTTCAGGAAGTGTCTTCCACCACTCCATGAACGTAGATTTCTTTAACAACATATCCTCTCCAATCTCATTGACCCATCTCTTCATCTTCTTCTCCAGCTCTATGATTTCTCTTGAATGCTCTACATACATTCTGGTTTGGGTTTGGATTTGAAGCTCCTCCACCATTGCCCAAAGAATCGATTCTTCACTGTATTCTGTCATCTTTCGATTATGTTCGAGCCATCTTTGTGTAAATCTGTAACGGTTCGGCCTTCCCATTTTCAGATAAGGACCAGTGTCATCATTCTTAGAGTGTCTGTAGTAATTAGCAATATCTAAAGGCTCAACGAGACGGCGATACTTGGTTCCAAGTTGTATCCATTCGTCATTGCCTTCAAATTCATCAGGGAGCTCATACATTTTGAGCATTCCTAGGATCTCATCCCATATACCTGCTTGCTCAAGCCTTTTGATATTGGCTTTGAAGTCTGTGTCATTCTTTTGGCGTTTGAATGCATCATAATATCCCATACCATCAACCCTACATACAGCTCTGTAGTTTTCTTCTAGCCAATTCAATCCTTGCTCGATATATTGCTTCTTTGCTTCCATCCTTTCTTGGTTTTTTGTCTTTTTCTCTTCGTATGTGCCAGCTGCACGAAGATTCAATAAAGCCCTCGTACTCTGTAA

mRNA sequence

CAAGGCCGGGAATTTAGGAAAACGGAGGGATATTTTGGTAAATATTTAAATCAAATCTCGGCCCTCTGTTTTTCCTAGTCTGTGGAAAAGAGCGAGCGAAGCAGAGTAGTTTCCGTTATCCATTATCTCTCTTTCTCCTTCCAAGGTCGTCTTCTCAATTATCCTCGCAAATTAGGGTTTTTCGCGAACTTCTTCCCTTCATTTCGATTTCAACTCTTCAATCATGGTTACTGATTCGATTACCAATGCTTCAGTTTCTTTGGCTCCGAACGCTGGGGATTTGCCCTTGCCCAAGAAGAAGAGGGTTAGGTATTCCAAATTGAAGCAGTCCAAGCTTGATGTTCGTCGGGAACAATGGCTTTCTCGAGGTGCTGTAAAGAACAAAAAATGGAACGAAGACAATCGTCTTGACTCAGTGGTCGTTAAAACGAGGAAAGATGATGATCTTTCCTCTAATAAAATAAATTCGAGGGAAATAGGAGAACAGAACAACAGATCGGTCCACCACCACAGCGATTTTTCAGAGTCGCCGTCTAATAGTCCCCCTAGTCTTGGGAGTAGTATCCTGTGTGGTAATGATTCGGTTCCTCACTTTACTGGGAGTAGCAGTAGTAGTAGTTGCCGAAGCAGCTCTAGTGGTTGTCAGTCGGGGAGTCTAACGGAGGAGGATGAAGGAGATGATGACTGCTTGGATGATTGGGAGGCTATTGCCGATGCCCTAGCCGCCACTGACAAGCAGCATGATCAATGTTCAGAGTCTTCCCCTAGCGGTAATATTGTTTCTCAATTGGATTCTTGCGGAGACAGCAGAAATGAGTTGGGCGTAGGAGATGGTGACTCGAGTATTGAACTCGGGAAAATTGTGCAGAGGGCTTCGATGAATTGCCGAGCATGGAGGCCTGATGATGCCTTTCGTCCTCAGAGTCTGCCTACGTTATCAAAGCAGCTTAGTTTACCGAATACAGACCGGCATTTTGGATATGGAGGCATCTCTTGGGCCTGTGGTGGTGTTATGCCTGTGCCTACATCTTGTCCAATTTGCTTTGAAGACTTAGACTTTACAGACTCGAGTTTCTTACCTTGTTTCTGTGGATTTCGGCTTTGCCTTTTCTGTCATAAAAGGATTCTTGAAGAGGATGGGCGCTGTCCTGGTTGCAGGAAGCCATATGATCGTGACCATGGTGACAATGAAACAAATGTAGATGGTGGTAGCCCAATGTTCCTGTTGGCACGTTCTTATAGCATGATTTCAAGGTCTTAGCTAGTGTCCAGTTTCTCATGTGGCAAGACTCCCAGGAGAATATACACGCTAAAATGAAATGTGGAAATATTTGTAATAACTTCTATGTCACACAACTAATTGTTCAATGCTTTTGATGTACATAGCCAGCACCAGCAGTTGAATGTCTGAGATCAGTCAAAGGATCTGAGGAGTGAACACTGAACGTGGATTGTTATAGAAGAATCTTTGTGGCGGGACATAGTTGCTGGAAGGTAGATTCTGTAATTGGTTGATTTTTCCACAGAAATAAATTTCACCATGTAAGCTTGTATATCATGTATTCATTAGGGCATTGGGTAGATCTGCCTTTGATTGAGGTCCCATTATCTGTTTAGCTTACGATGGATTGAATAATGTGATTGCTGAATAACTGCAGCTATGGGCTTTTCTAATTCTTTTGAACGAGAACAAGGGTGCTGATCTCAACTTATCTACAATTCTCTATGTATATTGGGCTCTCTGTCACATAGAGGTAATTATGAAGGATAATCCCATGGCATGTAGGTATGAGCCACTTCTATACTGGGTATTCATTTGCTGTTGATATTGACTTAGCTGGTGCAAGTTATTAAGAAGATATTGATTGTTGATGGGTGGTCTTTTTAGTTAAACTATAACAATTGCTGCGAAAGAATTGAAAGTGGATTCTGAAGAATAAATTATCTGATAGTCTAATTTTCATTAGGCTATGCCTTACGCTGGATCATATATCCTTGTTTATGCTGAGTTCTAGAAGACTAACCGACACTTGTCTCCAAGAATTTAAGTACTTAGTAAGATCAGACTACTCCCAGTTCATAGCATCGTCTCTTTTTTCCATCCTCTGAATATCATCCATAAGACAAGACTGTGATCTGTGTTGTTCAGGAAGTGTCTTCCACCACTCCATGAACGTAGATTTCTTTAACAACATATCCTCTCCAATCTCATTGACCCATCTCTTCATCTTCTTCTCCAGCTCTATGATTTCTCTTGAATGCTCTACATACATTCTGGTTTGGGTTTGGATTTGAAGCTCCTCCACCATTGCCCAAAGAATCGATTCTTCACTGTATTCTGTCATCTTTCGATTATGTTCGAGCCATCTTTGTGTAAATCTGTAACGGTTCGGCCTTCCCATTTTCAGATAAGGACCAGTGTCATCATTCTTAGAGTGTCTGTAGTAATTAGCAATATCTAAAGGCTCAACGAGACGGCGATACTTGGTTCCAAGTTGTATCCATTCGTCATTGCCTTCAAATTCATCAGGGAGCTCATACATTTTGAGCATTCCTAGGATCTCATCCCATATACCTGCTTGCTCAAGCCTTTTGATATTGGCTTTGAAGTCTGTGTCATTCTTTTGGCGTTTGAATGCATCATAATATCCCATACCATCAACCCTACATACAGCTCTGTAGTTTTCTTCTAGCCAATTCAATCCTTGCTCGATATATTGCTTCTTTGCTTCCATCCTTTCTTGGTTTTTTGTCTTTTTCTCTTCGTATGTGCCAGCTGCACGAAGATTCAATAAAGCCCTCGTACTCTGTAA

Coding sequence (CDS)

ATGGTTACTGATTCGATTACCAATGCTTCAGTTTCTTTGGCTCCGAACGCTGGGGATTTGCCCTTGCCCAAGAAGAAGAGGGTTAGGTATTCCAAATTGAAGCAGTCCAAGCTTGATGTTCGTCGGGAACAATGGCTTTCTCGAGGTGCTGTAAAGAACAAAAAATGGAACGAAGACAATCGTCTTGACTCAGTGGTCGTTAAAACGAGGAAAGATGATGATCTTTCCTCTAATAAAATAAATTCGAGGGAAATAGGAGAACAGAACAACAGATCGGTCCACCACCACAGCGATTTTTCAGAGTCGCCGTCTAATAGTCCCCCTAGTCTTGGGAGTAGTATCCTGTGTGGTAATGATTCGGTTCCTCACTTTACTGGGAGTAGCAGTAGTAGTAGTTGCCGAAGCAGCTCTAGTGGTTGTCAGTCGGGGAGTCTAACGGAGGAGGATGAAGGAGATGATGACTGCTTGGATGATTGGGAGGCTATTGCCGATGCCCTAGCCGCCACTGACAAGCAGCATGATCAATGTTCAGAGTCTTCCCCTAGCGGTAATATTGTTTCTCAATTGGATTCTTGCGGAGACAGCAGAAATGAGTTGGGCGTAGGAGATGGTGACTCGAGTATTGAACTCGGGAAAATTGTGCAGAGGGCTTCGATGAATTGCCGAGCATGGAGGCCTGATGATGCCTTTCGTCCTCAGAGTCTGCCTACGTTATCAAAGCAGCTTAGTTTACCGAATACAGACCGGCATTTTGGATATGGAGGCATCTCTTGGGCCTGTGGTGGTGTTATGCCTGTGCCTACATCTTGTCCAATTTGCTTTGAAGACTTAGACTTTACAGACTCGAGTTTCTTACCTTGTTTCTGTGGATTTCGGCTTTGCCTTTTCTGTCATAAAAGGATTCTTGAAGAGGATGGGCGCTGTCCTGGTTGCAGGAAGCCATATGATCGTGACCATGGTGACAATGAAACAAATGTAGATGGTGGTAGCCCAATGTTCCTGTTGGCACGTTCTTATAGCATGATTTCAAGGTCTTAG

Protein sequence

MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDNRLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDSVPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESSPSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS
Homology
BLAST of Cp4.1LG04g15230 vs. ExPASy Swiss-Prot
Match: O95628 (CCR4-NOT transcription complex subunit 4 OS=Homo sapiens OX=9606 GN=CNOT4 PE=1 SV=3)

HSP 1 Score: 70.9 bits (172), Expect = 3.3e-11
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = 0

Query: 267 PTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKRI-LEEDGRCPGCRKPYDRD 319
           P  CP+C E L+  D +F PC CG+++C FC  RI  +E+G CP CRKPY  D
Sbjct: 11  PVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPED 63

BLAST of Cp4.1LG04g15230 vs. ExPASy Swiss-Prot
Match: Q8BT14 (CCR4-NOT transcription complex subunit 4 OS=Mus musculus OX=10090 GN=Cnot4 PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 3.3e-11
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = 0

Query: 267 PTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKRI-LEEDGRCPGCRKPYDRD 319
           P  CP+C E L+  D +F PC CG+++C FC  RI  +E+G CP CRKPY  D
Sbjct: 11  PVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPED 63

BLAST of Cp4.1LG04g15230 vs. ExPASy Swiss-Prot
Match: P34909 (General negative regulator of transcription subunit 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MOT2 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 4.7e-10
Identity = 25/53 (47.17%), Postives = 36/53 (67.92%), Query Frame = 0

Query: 270 CPICFEDLDFTDSSFLPCFCGFRLCLFCHKRIL---EEDGRCPGCRKPYDRDH 320
           CP+C E +D TD +F PC CG+++C FC+  I    E +GRCP CR+ YD ++
Sbjct: 33  CPLCIEPMDITDKNFFPCPCGYQICQFCYNNIRQNPELNGRCPACRRKYDDEN 85

BLAST of Cp4.1LG04g15230 vs. ExPASy Swiss-Prot
Match: Q09818 (Putative general negative regulator of transcription C16C9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC16C9.04c PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.4e-09
Identity = 24/51 (47.06%), Postives = 36/51 (70.59%), Query Frame = 0

Query: 270 CPICFEDLDFTDSSFLPCFCGFRLCLFCHKRILEE-DGRCPGCRKPYDRDH 320
           CP+C E++D +D +F PC CG+R+C FC   I E+ +GRCP CR+ Y  ++
Sbjct: 18  CPLCMEEIDISDKNFKPCQCGYRVCRFCWHHIKEDLNGRCPACRRLYTEEN 68

BLAST of Cp4.1LG04g15230 vs. NCBI nr
Match: XP_023529902.1 (uncharacterized protein LOC111792615 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 698 bits (1802), Expect = 9.64e-254
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN
Sbjct: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
           RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS
Sbjct: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS 180
           VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS
Sbjct: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS 180

Query: 181 PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240
           PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK
Sbjct: 181 PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240

Query: 241 QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300
           QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR
Sbjct: 241 QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300

Query: 301 ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS
Sbjct: 301 ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345

BLAST of Cp4.1LG04g15230 vs. NCBI nr
Match: KAG7021482.1 (MOT-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 685 bits (1767), Expect = 2.09e-248
Identity = 337/345 (97.68%), Postives = 341/345 (98.84%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN
Sbjct: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
           RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS
Sbjct: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS 180
           VPHFTGSSSSSSCRSSSSGCQSGS+TEE+EGDDDCLDDWEAIADALAATDKQHDQCSESS
Sbjct: 121 VPHFTGSSSSSSCRSSSSGCQSGSITEEEEGDDDCLDDWEAIADALAATDKQHDQCSESS 180

Query: 181 PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240
           PSGN+VSQLDSCGDSRNELGVGDGDSSIELGKI QRASMNCRAWRPDDAFRPQSLPTLSK
Sbjct: 181 PSGNVVSQLDSCGDSRNELGVGDGDSSIELGKIAQRASMNCRAWRPDDAFRPQSLPTLSK 240

Query: 241 QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300
           QLSLPN DRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR
Sbjct: 241 QLSLPNADRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300

Query: 301 ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           ILEEDGRCPGCRKPYDR+H DNETNVDGGSP FLLARSYSMISRS
Sbjct: 301 ILEEDGRCPGCRKPYDREHVDNETNVDGGSPTFLLARSYSMISRS 345

BLAST of Cp4.1LG04g15230 vs. NCBI nr
Match: XP_022933340.1 (uncharacterized protein LOC111440610 [Cucurbita moschata])

HSP 1 Score: 681 bits (1758), Expect = 4.92e-247
Identity = 337/345 (97.68%), Postives = 341/345 (98.84%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN
Sbjct: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
           RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS
Sbjct: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS 180
           VPHFTGSSSSSSCRSSSSGCQSGS+TEE+EGDDDCLDDWEAIADALAATDKQHDQCSESS
Sbjct: 121 VPHFTGSSSSSSCRSSSSGCQSGSITEEEEGDDDCLDDWEAIADALAATDKQHDQCSESS 180

Query: 181 PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240
           PSGN+VSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK
Sbjct: 181 PSGNVVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240

Query: 241 QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300
           QLSLPN DRHFGYGGIS ACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR
Sbjct: 241 QLSLPNADRHFGYGGISLACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300

Query: 301 ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           ILEEDGRCPGCRKPYDR+H DNETNVDGGSP FLLARSYSMISRS
Sbjct: 301 ILEEDGRCPGCRKPYDREHVDNETNVDGGSPTFLLARSYSMISRS 345

BLAST of Cp4.1LG04g15230 vs. NCBI nr
Match: XP_022972777.1 (uncharacterized protein LOC111471280 [Cucurbita maxima])

HSP 1 Score: 675 bits (1742), Expect = 1.35e-244
Identity = 332/345 (96.23%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN
Sbjct: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
           RLDSVVVKTRKDDDLSSNK+NSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS
Sbjct: 61  RLDSVVVKTRKDDDLSSNKLNSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS 180
           VPHFTGSSSSSSCRSSSSGCQSGS+TEE+EGDDDCLDDWEAIADALAATDKQHDQCSESS
Sbjct: 121 VPHFTGSSSSSSCRSSSSGCQSGSITEEEEGDDDCLDDWEAIADALAATDKQHDQCSESS 180

Query: 181 PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240
           PS N+VSQLDSCGDSRNELG+GDGDSSIELGKI QRASMNCRAWRPDDAFRPQSLPTLSK
Sbjct: 181 PSANVVSQLDSCGDSRNELGIGDGDSSIELGKIAQRASMNCRAWRPDDAFRPQSLPTLSK 240

Query: 241 QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300
           QLSLPNTD+HFGYGGISWACGGVMPVP+SCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR
Sbjct: 241 QLSLPNTDQHFGYGGISWACGGVMPVPSSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300

Query: 301 ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           ILEEDGRCPGCRK YDRDH DNETNVD GSP FLLARSYSMISRS
Sbjct: 301 ILEEDGRCPGCRKSYDRDHVDNETNVDCGSPTFLLARSYSMISRS 345

BLAST of Cp4.1LG04g15230 vs. NCBI nr
Match: XP_004138290.2 (uncharacterized protein LOC101211244 [Cucumis sativus] >KAE8652409.1 hypothetical protein Csa_013954 [Cucumis sativus])

HSP 1 Score: 613 bits (1582), Expect = 3.79e-220
Identity = 308/349 (88.25%), Postives = 323/349 (92.55%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNE-D 60
           MVTDSI NAS+SLAPNA DLPLPKKKRVR SKLKQSKLDVRREQWLSRGAVKNKKWNE D
Sbjct: 1   MVTDSIANASISLAPNARDLPLPKKKRVRNSKLKQSKLDVRREQWLSRGAVKNKKWNEED 60

Query: 61  NRLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGND 120
           NRLDSVV+KTRKDDDLSS KIN REIGE+N+RSVHHHSD+S+SPSNSPPSLGSSIL GND
Sbjct: 61  NRLDSVVIKTRKDDDLSSEKINMREIGEENDRSVHHHSDYSDSPSNSPPSLGSSILGGND 120

Query: 121 SVPHFTGSSSSSSCRSSSSGCQSGSLTEEDE---GDDDCLDDWEAIADALAATDKQHDQC 180
           S PHFTGSSSSSSCRSSSSGC+SGS+TEE+E   GDDDCLDDWEAIADALAATDKQHDQC
Sbjct: 121 SGPHFTGSSSSSSCRSSSSGCRSGSITEEEEEEEGDDDCLDDWEAIADALAATDKQHDQC 180

Query: 181 SESSPSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLP 240
           SESSP GN++SQLDSCGD RNELGVGDGDSS+E G+IVQRASMNCRAWRPDDAFRPQSLP
Sbjct: 181 SESSPRGNVISQLDSCGDRRNELGVGDGDSSVERGRIVQRASMNCRAWRPDDAFRPQSLP 240

Query: 241 TLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLF 300
           TLSKQLSLP TDR FG GG+SWACGGV+PVPTSCPICFEDLD TDSSFLPCFCGFRLCLF
Sbjct: 241 TLSKQLSLPTTDRRFGCGGVSWACGGVIPVPTSCPICFEDLDLTDSSFLPCFCGFRLCLF 300

Query: 301 CHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           CHKRILEEDGRCPGCRKPYDRD  DNETNV  GSP   LARS SMISRS
Sbjct: 301 CHKRILEEDGRCPGCRKPYDRDPADNETNVLAGSPTLPLARSCSMISRS 349

BLAST of Cp4.1LG04g15230 vs. ExPASy TrEMBL
Match: A0A6J1F4L8 (uncharacterized protein LOC111440610 OS=Cucurbita moschata OX=3662 GN=LOC111440610 PE=4 SV=1)

HSP 1 Score: 681 bits (1758), Expect = 2.38e-247
Identity = 337/345 (97.68%), Postives = 341/345 (98.84%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN
Sbjct: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
           RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS
Sbjct: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS 180
           VPHFTGSSSSSSCRSSSSGCQSGS+TEE+EGDDDCLDDWEAIADALAATDKQHDQCSESS
Sbjct: 121 VPHFTGSSSSSSCRSSSSGCQSGSITEEEEGDDDCLDDWEAIADALAATDKQHDQCSESS 180

Query: 181 PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240
           PSGN+VSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK
Sbjct: 181 PSGNVVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240

Query: 241 QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300
           QLSLPN DRHFGYGGIS ACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR
Sbjct: 241 QLSLPNADRHFGYGGISLACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300

Query: 301 ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           ILEEDGRCPGCRKPYDR+H DNETNVDGGSP FLLARSYSMISRS
Sbjct: 301 ILEEDGRCPGCRKPYDREHVDNETNVDGGSPTFLLARSYSMISRS 345

BLAST of Cp4.1LG04g15230 vs. ExPASy TrEMBL
Match: A0A6J1I5R9 (uncharacterized protein LOC111471280 OS=Cucurbita maxima OX=3661 GN=LOC111471280 PE=4 SV=1)

HSP 1 Score: 675 bits (1742), Expect = 6.54e-245
Identity = 332/345 (96.23%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN
Sbjct: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
           RLDSVVVKTRKDDDLSSNK+NSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS
Sbjct: 61  RLDSVVVKTRKDDDLSSNKLNSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESS 180
           VPHFTGSSSSSSCRSSSSGCQSGS+TEE+EGDDDCLDDWEAIADALAATDKQHDQCSESS
Sbjct: 121 VPHFTGSSSSSSCRSSSSGCQSGSITEEEEGDDDCLDDWEAIADALAATDKQHDQCSESS 180

Query: 181 PSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSK 240
           PS N+VSQLDSCGDSRNELG+GDGDSSIELGKI QRASMNCRAWRPDDAFRPQSLPTLSK
Sbjct: 181 PSANVVSQLDSCGDSRNELGIGDGDSSIELGKIAQRASMNCRAWRPDDAFRPQSLPTLSK 240

Query: 241 QLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300
           QLSLPNTD+HFGYGGISWACGGVMPVP+SCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR
Sbjct: 241 QLSLPNTDQHFGYGGISWACGGVMPVPSSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKR 300

Query: 301 ILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           ILEEDGRCPGCRK YDRDH DNETNVD GSP FLLARSYSMISRS
Sbjct: 301 ILEEDGRCPGCRKSYDRDHVDNETNVDCGSPTFLLARSYSMISRS 345

BLAST of Cp4.1LG04g15230 vs. ExPASy TrEMBL
Match: A0A1S3BVJ1 (uncharacterized protein LOC103493947 OS=Cucumis melo OX=3656 GN=LOC103493947 PE=4 SV=1)

HSP 1 Score: 607 bits (1565), Expect = 7.15e-218
Identity = 305/349 (87.39%), Postives = 322/349 (92.26%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNE-D 60
           MVTDSI NAS+SLAPNA DLPLPKKKRVR SKLKQSKLDVRREQWLSRGAVKNKKWNE D
Sbjct: 1   MVTDSIANASISLAPNARDLPLPKKKRVRNSKLKQSKLDVRREQWLSRGAVKNKKWNEED 60

Query: 61  NRLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGND 120
           NRLDSVV+KTRKDD+LSS KIN REIGE+N+RSVHHHSD+S+SPSNSPPSLGSSIL GND
Sbjct: 61  NRLDSVVIKTRKDDNLSSEKINMREIGEENDRSVHHHSDYSDSPSNSPPSLGSSILGGND 120

Query: 121 SVPHFTGSSSSSSCRSSSSGCQSGSLTEEDE---GDDDCLDDWEAIADALAATDKQHDQC 180
           S PHFTGSSSSSSCRSSSSGC+SGS+TEE+E   GDDDCLDDWEAIADALAATDKQ DQC
Sbjct: 121 SGPHFTGSSSSSSCRSSSSGCRSGSITEEEEEEEGDDDCLDDWEAIADALAATDKQRDQC 180

Query: 181 SESSPSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLP 240
           SESSP GN+VSQLDSCGD RNELGVGDGDS++E G+IVQRASMNCRAWRPDDAFRPQSLP
Sbjct: 181 SESSPRGNVVSQLDSCGDRRNELGVGDGDSNVERGRIVQRASMNCRAWRPDDAFRPQSLP 240

Query: 241 TLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLF 300
           TLSKQLSLPNTDR FG GG+ WACGGV+PVPTSCPICFEDLD TDSSFLPCFCGFRLCLF
Sbjct: 241 TLSKQLSLPNTDRRFGCGGVPWACGGVLPVPTSCPICFEDLDLTDSSFLPCFCGFRLCLF 300

Query: 301 CHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 345
           CHKRILEEDGRCPGCRKPYDRD  DNETN+  GSP   LARS SMISRS
Sbjct: 301 CHKRILEEDGRCPGCRKPYDRDPADNETNILVGSPTLPLARSCSMISRS 349

BLAST of Cp4.1LG04g15230 vs. ExPASy TrEMBL
Match: A0A5A7UPV5 (Suppressor protein SRP40 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002030 PE=4 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 3.67e-216
Identity = 304/348 (87.36%), Postives = 321/348 (92.24%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNE-D 60
           MVTDSI NAS+SLAPNA DLPLPKKKRVR SKLKQSKLDVRREQWLSRGAVKNKKWNE D
Sbjct: 1   MVTDSIANASISLAPNARDLPLPKKKRVRNSKLKQSKLDVRREQWLSRGAVKNKKWNEED 60

Query: 61  NRLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGND 120
           NRLDSVV+KTRKDD+LSS KIN REIGE+N+RSVHHHSD+S+SPSNSPPSLGSSIL GND
Sbjct: 61  NRLDSVVIKTRKDDNLSSEKINMREIGEENDRSVHHHSDYSDSPSNSPPSLGSSILGGND 120

Query: 121 SVPHFTGSSSSSSCRSSSSGCQSGSLTEEDE---GDDDCLDDWEAIADALAATDKQHDQC 180
           S PHFTGSSSSSSCRSSSSGC+SGS+TEE+E   GDDDCLDDWEAIADALAATDKQ DQC
Sbjct: 121 SGPHFTGSSSSSSCRSSSSGCRSGSITEEEEEEEGDDDCLDDWEAIADALAATDKQRDQC 180

Query: 181 SESSPSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLP 240
           SESSP GN+VSQLDSCGD RNELGVGDGDS++E G+IVQRASMNCRAWRPDDAFRPQSLP
Sbjct: 181 SESSPRGNVVSQLDSCGDRRNELGVGDGDSNVERGRIVQRASMNCRAWRPDDAFRPQSLP 240

Query: 241 TLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLF 300
           TLSKQLSLPNTDR FG GG+ WACGGV+PVPTSCPICFEDLD TDSSFLPCFCGFRLCLF
Sbjct: 241 TLSKQLSLPNTDRRFGCGGVPWACGGVLPVPTSCPICFEDLDLTDSSFLPCFCGFRLCLF 300

Query: 301 CHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISR 344
           CHKRILEEDGRCPGCRKPYDRD  DNETN+  GSP   LARS SMISR
Sbjct: 301 CHKRILEEDGRCPGCRKPYDRDPADNETNILVGSPTLPLARSCSMISR 348

BLAST of Cp4.1LG04g15230 vs. ExPASy TrEMBL
Match: A0A5D3BIS7 (Suppressor protein SRP40 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002110 PE=4 SV=1)

HSP 1 Score: 603 bits (1556), Expect = 2.29e-214
Identity = 303/347 (87.32%), Postives = 320/347 (92.22%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNE-D 60
           MVTDSI NAS+SLAPNA DLPLPKKKRVR SKLKQSKLDVRREQWLSRGAVKNKKWNE D
Sbjct: 1   MVTDSIANASISLAPNARDLPLPKKKRVRNSKLKQSKLDVRREQWLSRGAVKNKKWNEED 60

Query: 61  NRLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGND 120
           NRLDSVV+KTRKDD+LSS KIN REIGE+N+RSVHHHSD+S+SPSNSPPSLGSSIL GND
Sbjct: 61  NRLDSVVIKTRKDDNLSSEKINMREIGEENDRSVHHHSDYSDSPSNSPPSLGSSILGGND 120

Query: 121 SVPHFTGSSSSSSCRSSSSGCQSGSLTEEDE---GDDDCLDDWEAIADALAATDKQHDQC 180
           S PHFTGSSSSSSCRSSSSGC+SGS+TEE+E   GDDDCLDDWEAIADALAATDKQ DQC
Sbjct: 121 SGPHFTGSSSSSSCRSSSSGCRSGSITEEEEEEEGDDDCLDDWEAIADALAATDKQRDQC 180

Query: 181 SESSPSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLP 240
           SESSP GN+VSQLDSCGD RNELGVGDGDS++E G+IVQRASMNCRAWRPDDAFRPQSLP
Sbjct: 181 SESSPRGNVVSQLDSCGDRRNELGVGDGDSNVERGRIVQRASMNCRAWRPDDAFRPQSLP 240

Query: 241 TLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLF 300
           TLSKQLSLPNTDR FG GG+ WACGGV+PVPTSCPICFEDLD TDSSFLPCFCGFRLCLF
Sbjct: 241 TLSKQLSLPNTDRRFGCGGVPWACGGVLPVPTSCPICFEDLDLTDSSFLPCFCGFRLCLF 300

Query: 301 CHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMIS 343
           CHKRILEEDGRCPGCRKPYDRD  DNETN+  GSP   LARS SMIS
Sbjct: 301 CHKRILEEDGRCPGCRKPYDRDPADNETNILVGSPTLPLARSCSMIS 347

BLAST of Cp4.1LG04g15230 vs. TAIR 10
Match: AT3G48070.1 (RING/U-box superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 5.6e-67
Identity = 163/351 (46.44%), Postives = 209/351 (59.54%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           M++DSITNAS + AP A D    KK+  R +KLKQSKL +RREQWLS+ A+ NK   E+ 
Sbjct: 1   MISDSITNASATSAPTARDFG-KKKRTNRSAKLKQSKLGLRREQWLSQVAMINKGDKEEM 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
             +  +   + D       + +    +++N   H H  F ES SNSP    +SIL G +S
Sbjct: 61  ESNRRIGSEKPDQRDLPRPVENL---DEDNNGTHRHESFIESLSNSP----NSILSGMNS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDD-CLDDWEAIADALAATDKQHDQCSES 180
           +P+F+ SSS     S S G  SG++TEE++ DDD CLDDWEAIADALAA D++H++  E+
Sbjct: 121 IPNFSSSSS-----SGSGGSCSGNITEEEDADDDGCLDDWEAIADALAADDEKHEK--EN 180

Query: 181 SPSGNIVSQLDSC---GDSRNELGVGDGDSSIELGKI--VQRASMNCRAWRPDDAFRPQS 240
            P        +SC    D +    +G  D  +   K    +R   + +AWRPDD  RPQ 
Sbjct: 181 PP--------ESCQEHEDIKQSALIGGADVVLRDAKADSQRRKQKSNQAWRPDDKLRPQG 240

Query: 241 LPTLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLC 300
           LP L KQ S P  + HF           V  VP+SCPIC+EDLD TDS+FLPC CGFRLC
Sbjct: 241 LPNLEKQRSFPVMNLHF---------SSVTVVPSSCPICYEDLDLTDSNFLPCPCGFRLC 300

Query: 301 LFCHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISRS 346
           LFCHK I + DGRCPGCRKPY+R+    E +V GG     LARS SM  RS
Sbjct: 301 LFCHKTICDGDGRCPGCRKPYERNTIKFEASVQGGGLTIRLARSSSMFCRS 319

BLAST of Cp4.1LG04g15230 vs. TAIR 10
Match: AT3G48070.2 (RING/U-box superfamily protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.6e-66
Identity = 162/350 (46.29%), Postives = 208/350 (59.43%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           M++DSITNAS + AP A D    KK+  R +KLKQSKL +RREQWLS+ A+ NK   E+ 
Sbjct: 1   MISDSITNASATSAPTARDFG-KKKRTNRSAKLKQSKLGLRREQWLSQVAMINKGDKEEM 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
             +  +   + D       + +    +++N   H H  F ES SNSP    +SIL G +S
Sbjct: 61  ESNRRIGSEKPDQRDLPRPVENL---DEDNNGTHRHESFIESLSNSP----NSILSGMNS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEEDEGDDD-CLDDWEAIADALAATDKQHDQCSES 180
           +P+F+ SSS     S S G  SG++TEE++ DDD CLDDWEAIADALAA D++H++  E+
Sbjct: 121 IPNFSSSSS-----SGSGGSCSGNITEEEDADDDGCLDDWEAIADALAADDEKHEK--EN 180

Query: 181 SPSGNIVSQLDSC---GDSRNELGVGDGDSSIELGKI--VQRASMNCRAWRPDDAFRPQS 240
            P        +SC    D +    +G  D  +   K    +R   + +AWRPDD  RPQ 
Sbjct: 181 PP--------ESCQEHEDIKQSALIGGADVVLRDAKADSQRRKQKSNQAWRPDDKLRPQG 240

Query: 241 LPTLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPCFCGFRLC 300
           LP L KQ S P  + HF           V  VP+SCPIC+EDLD TDS+FLPC CGFRLC
Sbjct: 241 LPNLEKQRSFPVMNLHF---------SSVTVVPSSCPICYEDLDLTDSNFLPCPCGFRLC 300

Query: 301 LFCHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISR 345
           LFCHK I + DGRCPGCRKPY+R+    E +V GG     LARS SM  R
Sbjct: 301 LFCHKTICDGDGRCPGCRKPYERNTIKFEASVQGGGLTIRLARSSSMFCR 318

BLAST of Cp4.1LG04g15230 vs. TAIR 10
Match: AT5G62910.1 (RING/U-box superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-64
Identity = 161/357 (45.10%), Postives = 212/357 (59.38%), Query Frame = 0

Query: 1   MVTDSITNASVSLAPNAGDLPLPKKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNEDN 60
           M+TDSITNAS + AP        KK+  + +K+KQ+KL +RREQWLS+ AV NK+  E+ 
Sbjct: 1   MITDSITNASATSAPRDSG---KKKRNNKSAKMKQNKLGLRREQWLSQVAVSNKEVKEER 60

Query: 61  RLDSVVVKTRKDDDLSSNKINSREIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDS 120
            ++    +++K    SS+K+   E     N  +HH S F ESPSNS  S+G     G  S
Sbjct: 61  SVN----RSQKPHHESSDKVRREEDNNGGNNLLHHES-FMESPSNS--SVG-----GTYS 120

Query: 121 VPHFTGSSSSSSCRSSSSGCQSGSLTEE---DEGDDDCLDDWEAIADALAATDK------ 180
             +F+G SS SS  SSSSG  SG++TEE   D+ DD C+DDWEA+ADALAA ++      
Sbjct: 121 STNFSGRSSRSS--SSSSGFCSGNITEEENVDDDDDGCVDDWEAVADALAAEEEIEKKSR 180

Query: 181 ----QHDQCSESSPSGNIVSQLDSCGDSRNELGVGDGDSSIELGKIVQRASMNCRAWRPD 240
                 +Q S    + N+     S  D+ + +GV D     E  ++  R   + RAWR D
Sbjct: 181 PLESVKEQVSVGQSASNVCD--SSISDASDVVGVEDPKQ--ECLRVSSRKQTSNRAWRLD 240

Query: 241 DAFRPQSLPTLSKQLSLPNTDRHFGYGGISWACGGVMPVPTSCPICFEDLDFTDSSFLPC 300
           D  RPQ LP L+KQLS P  D+ F            + +P+SCPIC+EDLD TDS+FLPC
Sbjct: 241 DDLRPQGLPNLAKQLSFPELDKRF----------SSVAIPSSCPICYEDLDLTDSNFLPC 300

Query: 301 FCGFRLCLFCHKRILEEDGRCPGCRKPYDRDHGDNETNVDGGSPMFLLARSYSMISR 345
            CGFRLCLFCHK I + DGRCPGCRKPY+R+    ET++ GG     LARS SM  +
Sbjct: 301 PCGFRLCLFCHKTICDGDGRCPGCRKPYERNMVKAETSIQGGGLTIRLARSSSMFCK 326

BLAST of Cp4.1LG04g15230 vs. TAIR 10
Match: AT1G74870.1 (RING/U-box superfamily protein )

HSP 1 Score: 85.1 bits (209), Expect = 1.2e-16
Identity = 86/295 (29.15%), Postives = 129/295 (43.73%), Query Frame = 0

Query: 24  KKKRVRYSKLKQSKLDVRREQWLSRGAVKNKKWNE-DNRLDSVVVKTRKDDDLSSNKINS 83
           K+K   + KLKQ K+D RR+QW+S+    N    E   RL S++ K     D  + +I+ 
Sbjct: 22  KRKANWFCKLKQWKIDARRKQWISQWKKANVDEEEIGRRLRSLLEKL---TDQKAWRIDY 81

Query: 84  REIGEQNNRSVHHHSDFSESPSNSPPSLGSSILCGNDSVPHFTGSSSSSSCRSSSSGCQS 143
            +  E +   +   S F+ SP        +S+L   DSV      S    C      C  
Sbjct: 82  DD-DEDDEIDLERTSSFASSP--------TSVLKRKDSV------SGDCFC------CSK 141

Query: 144 GSLTEEDEGDDDCLDDWEAIADALAATDKQHDQCSESSPSGNIVSQLDSCGDSRNELGVG 203
               EE+E  DD  D+W+   DAL + +      ++++ S  +V++     D   E    
Sbjct: 142 QMTEEEEEVFDDAYDNWDGFKDALNSFE------NDNNESSRLVTE-----DFEQEEEDL 201

Query: 204 DGDSSIELGKIVQRASMNCRAWRPDDAFRPQSLPTLSKQLSLPNTDRHFGYGGISWACGG 263
             D+S  + K  Q A+               ++   S +    N+++  G G        
Sbjct: 202 IPDTSQRMNKCKQEAAPG----------NQTTIHRNSNKKKRSNSEKQRGDG-------- 259

Query: 264 VMPVPTSCPICFEDLDFTDSSFLPCFCGFRLCLFCHKRILEEDGRCPGCRKPYDR 318
                  CPIC E +D TD  F PC CGFR+CLFCH +I E + RCP CRK Y +
Sbjct: 262 ----DEECPICSELMDATDLEFEPCTCGFRICLFCHNKISENEARCPACRKDYKK 259

BLAST of Cp4.1LG04g15230 vs. TAIR 10
Match: AT3G45630.1 (RNA binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 65.9 bits (159), Expect = 7.5e-11
Identity = 23/56 (41.07%), Postives = 37/56 (66.07%), Query Frame = 0

Query: 269 SCPICFEDLDFTDSSFLPCFCGFRLCLFCHKRIL------EEDGRCPGCRKPYDRD 319
           +CP+C E++D TD    PC CG+++C++C   I+      + +GRCP CR PYD++
Sbjct: 8   TCPLCAEEMDLTDQQLKPCKCGYQICVWCWHHIMDMAEKDQSEGRCPACRTPYDKE 63

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O956283.3e-1150.94CCR4-NOT transcription complex subunit 4 OS=Homo sapiens OX=9606 GN=CNOT4 PE=1 S... [more]
Q8BT143.3e-1150.94CCR4-NOT transcription complex subunit 4 OS=Mus musculus OX=10090 GN=Cnot4 PE=1 ... [more]
P349094.7e-1047.17General negative regulator of transcription subunit 4 OS=Saccharomyces cerevisia... [more]
Q098181.4e-0947.06Putative general negative regulator of transcription C16C9.04c OS=Schizosaccharo... [more]
Match NameE-valueIdentityDescription
XP_023529902.19.64e-254100.00uncharacterized protein LOC111792615 [Cucurbita pepo subsp. pepo][more]
KAG7021482.12.09e-24897.68MOT-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022933340.14.92e-24797.68uncharacterized protein LOC111440610 [Cucurbita moschata][more]
XP_022972777.11.35e-24496.23uncharacterized protein LOC111471280 [Cucurbita maxima][more]
XP_004138290.23.79e-22088.25uncharacterized protein LOC101211244 [Cucumis sativus] >KAE8652409.1 hypothetica... [more]
Match NameE-valueIdentityDescription
A0A6J1F4L82.38e-24797.68uncharacterized protein LOC111440610 OS=Cucurbita moschata OX=3662 GN=LOC1114406... [more]
A0A6J1I5R96.54e-24596.23uncharacterized protein LOC111471280 OS=Cucurbita maxima OX=3661 GN=LOC111471280... [more]
A0A1S3BVJ17.15e-21887.39uncharacterized protein LOC103493947 OS=Cucumis melo OX=3656 GN=LOC103493947 PE=... [more]
A0A5A7UPV53.67e-21687.36Suppressor protein SRP40 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3BIS72.29e-21487.32Suppressor protein SRP40 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT3G48070.15.6e-6746.44RING/U-box superfamily protein [more]
AT3G48070.21.6e-6646.29RING/U-box superfamily protein [more]
AT5G62910.11.5e-6445.10RING/U-box superfamily protein [more]
AT1G74870.11.2e-1629.15RING/U-box superfamily protein [more]
AT3G45630.17.5e-1141.07RNA binding (RRM/RBD/RNP motifs) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 266..326
e-value: 3.3E-17
score: 63.6
NoneNo IPR availablePFAMPF14570zf-RING_4coord: 270..316
e-value: 7.2E-18
score: 64.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 99..114
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..145
NoneNo IPR availablePANTHERPTHR12603:SF7RING/U-BOX SUPERFAMILY PROTEINcoord: 7..343
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 268..319
IPR039780CCR4-NOT transcription complex subunit 4PANTHERPTHR12603CCR4-NOT TRANSCRIPTION COMPLEX RELATEDcoord: 7..343
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 270..312
score: 9.096088
IPR039515NOT4, modified RING finger, HC subclass (C4C4-type)CDDcd16618mRING-HC-C4C4_CNOT4coord: 270..313
e-value: 3.77347E-18
score: 74.9584

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g15230.1Cp4.1LG04g15230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0030014 CCR4-NOT complex
molecular_function GO:0004842 ubiquitin-protein transferase activity