Cp4.1LG04g10180 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g10180
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Lactoylglutathione lyase
Location	Cp4.1LG04: 9632015 .. 9636256 (+)
RNA-Seq Expression	Cp4.1LG04g10180
Synteny	Cp4.1LG04g10180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGAGACAGAGTGATCTTTTTCTGGCGGGTGAAAGCTTTTGGCATATCCCGCAATCGCTAGAACTCGCTGTCTTCTTCTTCTTCCTCTTACTGCGAGGGCTCAAAGTTGCGGAAAATCTCTAGAAGATACGGGAAAGAGAAGAAAAACAGAAACGAAGATTGGAAATTGAGTTTGGAGAACCTGTTTCGCAACTAGTTATTCTGTTCATCACTTGGGCTTTCACAGTTCGTGCTGGAATTGAGCCGAAGTGACGGCGCAGAGTTATGGTGAGGATATTACCAATGGCTTCCACATTGCGACCTTCCATTTCGTCATTTAGGTTTGCTGCTGCCTCTCGTTTGGCTCATTCTCTCACTTCTTACACCCCTACTCGGAGGCTGCCTTCATTTCATCTCGGTTTTGGTGCGCCTTTAGTCTCTTCACTGAGCCCTGGATTTAAATGCTAATTTTTGTACTCCTCAATATGTTTCTGTATTGTTTTATCGTGTCGTTTTGATTTGGCGGCAGTAATTGGTCGGTTCCGGGATTTTTCCGGCTTATGCCAATTGGTTGTGTTCTGTTGAAAGCTTTCCTATCACATTCATAGTTGGATTGCTATGCTGTTTTAGCACGATGCCTTGCGTAGCTTCCTGTTCCTGGGTGTTTTCCACCGTCACTAAAGACTAAACTGAAATAGAAAAATTAGGAAAAAAGCTGAGAGTTGGAAATTTTCATTCATGTAGCAGCATTTATGCATCACTTTCTGGTTTGAAATCTGGAGTTCTTATGAAGATAGATGATATTTTACGGTTGATATCTCAATCCTGCAGCTTTTCAAACCATGTTTTCTATGTGAAGTCTTTTGGTTCGATGGTTTCTAGTTTGTTAATTGTATTTGCTAACTTGGATTCCTCATTTGGTATGAAGACATGAATTTTTTTGTGTTACTCCAGACATAAATGTCTCGGGAGAATGATTTTAAGTTGTTGTATAGCTGTTCCCCAGGCCCAATTCTTTGGTCTAAACACTTCGAAGTTGTGGCAAAAGGAGCATAATAACACCATAAGGGTGGCCATAAATGGATCTGTAGCACAGGCTAGCACAACAACCAGTCCAGAAAATATTCTTGAGTGGGTGAAAAAGGATAAGAGGAGATTTCTTCATGTTGTCTATCGTGTTGGAGATTTGGAGAGGACCATAAAGTATGTATAATTTCCAAGAGACTTAAAGTTTCTTGCTACATCTTATTATCTCTTTTCCCGTCTTCCTAAGACAATTCAATTACAAAATATGTTTACTGCAGATTCTACACTGAGTGCCTGGGTATGAAGCTGCTACGAAAACGTGACATACCAGAGGAAAGATATACAAATGCATTTCTTGGATACGGTCCTGAAGATGCTCACTTTGTTATTGAACTCACTTACAGTAAGTAGTCTACGTGACTTTATCCAATATTTCACCCCCATGAATTGACTAGATCAATCAAATTGATTTATTTTGATTGACTACTGATCACAGATTATGGAGTTGACAAGTATGATATTGGAACCGGATTCGGACATTTTGGTATTGCAGTTGAAGATGTAAGTCTATGCTTTATTTTTTTATTTTTCTATGTTATACGCTTGATTTATACCATATAAATTTATTTACTCTTGTGTCAACAGTCCCTTCTACTTTGTGCTTTTGGCCTCATACCCTTCAAATCATTTTTTTAGTTACCAGCTTTTATCATGGTTTTTTATTCCCACTTTCTACCACTAGGTTTCGAGGACGGTGGAACTTATAAAGGCCAAGGGTGGAAAGGTTACCAGAGAACCTGGTCCTGTTAAAGGTGGCAGTACTGTGATTGCCTTTATCGAAGATCCAGATGGCTATAAGTTTGAACTCTTGGAAAGGGGGCCTACTCCCGAGCCTTTATGTCAAGTAATGCTTCGTGTAGGTGATCTTGATCGATCCATAAATTTTTATGAGAAGGTGAGACTTGTTTGAATGCTATATTGGGCTGTCTAAATGTTCCTCTTTTTTTTTTTTTCTTTCTTATAGTTAGTTTTGTTTGATTGTTTTTCCTTCTCAATACCTTTGTCTGATACGTATTCCGAATCATGTGCATTTTATGTAAGGCTTTTGGCATGGAAGTCCTTCGCAAACGAGATAATCCAGATTATAAGGTAATATGGTATTTTTGTTAGATTTGTTTTCCTACAAGTTCTAAGATAGTTTTCTAATTTCAGAAGATTCTGCAATCTCTGATGGAAAATTTTTGTTTCTTCAATAGCCTGGGCCTCCCGCCGGGGCCCGCTATGTCAAAAATGAGAGAAACCACATCCCTCTCTTTCCTTTTTTTTTTTTTTGCCAGTATGTGTCACATGGGAGGGACATGGGGACTCAAAAAATGGATCCTATCAACTTGTTCCGACCTAGGATAATAAGCTCACACACACATGCATGCTGATGCTTTGATATATGTATTCCATGTGATACTTTTATATTAAGTAACTTCTTTTGATTTTACATGCAACTTTCAGTACACGATAGCAATGATGGGATATGGCCCTGAAGATAAAAATCCTGTACTGGAGTTGACTTACAACTATGGAGTTACTGACTACGAAAAAGGCAATGGTTATGCCCAGGTTTAACATTTACACTTGTGTTCCTTTTGTTTTTGCTATTGGGATAGTAGAAAGAAAAACCTGATGGATGTCTTGCTGTATTTGTAATCTAGTGGAACAATGTTTGGGTATAATTATTTCTTTCCTTTTGACAGATTGCAATAGGCACAGACGATGTCTATAAAACTGCAGAAGCAGTAAAACTTTATGGAGGGAAGGTCACCCGAGAACCTGGACCATTACCTGGTATCAACACCAAAATTACTGCATGCTTGGATCCTGATGGTTGGAAAACGGTACGACTTTCGTATTCAATCCTTGTAGGCTTCAAAATATGCATGAAATCTCTTGATTAACCTTTCGCTCAGCATCTATAGAACTGATTTAGTATTGACCCAGATTCCTCGTTATGGTTCCATTGACTTTCACTGTGCACGTGAGCGAACATGGTAAAAGATGCTCCAACTCAATCTAGTTATGCACCAGATACTGTGCACATGAGCTTTATAACTATAATGATTCAAATCAATCTAGTTATGCATGTTCAGTTCTTATTTAAACTCAGCTACGAGAAAGTCTCATGAAAACCTTCCGAGTATGTGACCTTGTGTCTTGTTTGTGATTCAAACCAATCAAGTCTTGCATGCCTTTGCTGAGAAAATAGTCATTCAAACCTTTAATCACTGAGTTTCTTTATAAGTAGCAGAAAATTGTTTTACACAACGCACTCAAATTGCCGCGTATCTTGTGTTTTAAAAAGCAGAGATCTCGAGGGGAATAAATGCTTCTTCCATCTAGATTTTGATTTTCTTGTAAGGGTAGAACATACCCTGCATATTGATCTTGGTCGCTCAAAATATAACTAAGTGAGGAAACAGAAGAAGAACAATGATCGCAAGTATAATTCTCTCTTATAGGAAAGTGATCAAGAGTGGACACATATTTATAGAAGTGGCTGCTTAAATGAGTTATAACTATCATAACAGTAATTTGGTTTTATTATAACAAAATAATAAAGAAAATGAATAGTCGGTTCAGTTGCCATATCAATCTGGCTGTGCTGTTTTTCACTCTTTGGTACTGATTAGTCGACTTCTCATTTGTTCTCTTATGATCTGCAGGTTTTTGTAGACAACATCGATTTTCTTAAGGAGTTGGAGTGAGAGAATCATGGAATTAATGCCTGCCAGTTCCGTGCCAGTTGAGACCCAAGCCCAGCTTGAAAATAATTCTTTATGCTTGCGTCTAGCTTCGCGCTGATTGAATATAGAAATTGTCTGTATATGCCATGATGTCTTGATAATTTGCTATTAGGCTTGCATAATGATGATCAAGTGTATTTTAGGGTTTGAAAGGCTTACCTGCATGAGTTAAAATGAATAAACTTACAGTTCTCTGTAATGTTTATTTATTGATTTTAACAGCAGAGCTGTGGCATAAAATTTTTGACACCCAACTTAAGTTTAGTTGTATGGTTCAGAATTCAAGTTCCATTCCCCAGAGATGTGTCGGTTGGCTCTGCGGCTATTGACTTGATTGATTCAAAGTGAGTAATTGATATATATAAGAAAATTTGGTAACTTTATTTTATGTGTAAAGGCTAGATTTTGTGTAAGAATCGAGTTATGTTGGAGGCAATTTGAT

mRNA sequence

GCGAGACAGAGTGATCTTTTTCTGGCGGGTGAAAGCTTTTGGCATATCCCGCAATCGCTAGAACTCGCTGTCTTCTTCTTCTTCCTCTTACTGCGAGGGCTCAAAGTTGCGGAAAATCTCTAGAAGATACGGGAAAGAGAAGAAAAACAGAAACGAAGATTGGAAATTGAGTTTGGAGAACCTGTTTCGCAACTAGTTATTCTGTTCATCACTTGGGCTTTCACAGTTCGTGCTGGAATTGAGCCGAAGTGACGGCGCAGAGTTATGGTGAGGATATTACCAATGGCTTCCACATTGCGACCTTCCATTTCGTCATTTAGGTTTGCTGCTGCCTCTCGTTTGGCTCATTCTCTCACTTCTTACACCCCTACTCGGAGGCTGCCTTCATTTCATCTCGGTTTTGCTGTTCCCCAGGCCCAATTCTTTGGTCTAAACACTTCGAAGTTGTGGCAAAAGGAGCATAATAACACCATAAGGGTGGCCATAAATGGATCTGTAGCACAGGCTAGCACAACAACCAGTCCAGAAAATATTCTTGAGTGGGTGAAAAAGGATAAGAGGAGATTTCTTCATGTTGTCTATCGTGTTGGAGATTTGGAGAGGACCATAAAATTCTACACTGAGTGCCTGGGTATGAAGCTGCTACGAAAACGTGACATACCAGAGGAAAGATATACAAATGCATTTCTTGGATACGGTCCTGAAGATGCTCACTTTGTTATTGAACTCACTTACAATTATGGAGTTGACAAGTATGATATTGGAACCGGATTCGGACATTTTGGTATTGCAGTTGAAGATGTTTCGAGGACGGTGGAACTTATAAAGGCCAAGGGTGGAAAGGTTACCAGAGAACCTGGTCCTGTTAAAGGTGGCAGTACTGTGATTGCCTTTATCGAAGATCCAGATGGCTATAAGTTTGAACTCTTGGAAAGGGGGCCTACTCCCGAGCCTTTATGTCAAGTAATGCTTCGTGTAGGTGATCTTGATCGATCCATAAATTTTTATGAGAAGGCTTTTGGCATGGAAGTCCTTCGCAAACGAGATAATCCAGATTATAAGTACACGATAGCAATGATGGGATATGGCCCTGAAGATAAAAATCCTGTACTGGAGTTGACTTACAACTATGGAGTTACTGACTACGAAAAAGGCAATGGTTATGCCCAGGTTTTTGTAGACAACATCGATTTTCTTAAGGAGTTGGAGTGAGAGAATCATGGAATTAATGCCTGCCAGTTCCGTGCCAGTTGAGACCCAAGCCCAGCTTGAAAATAATTCTTTATGCTTGCGTCTAGCTTCGCGCTGATTGAATATAGAAATTGTCTGTATATGCCATGATGTCTTGATAATTTGCTATTAGGCTTGCATAATGATGATCAAGTGTATTTTAGGGTTTGAAAGGCTTACCTGCATGAGTTAAAATGAATAAACTTACAGTTCTCTGTAATGTTTATTTATTGATTTTAACAGCAGAGCTGTGGCATAAAATTTTTGACACCCAACTTAAGTTTAGTTGTATGGTTCAGAATTCAAGTTCCATTCCCCAGAGATGTGTCGGTTGGCTCTGCGGCTATTGACTTGATTGATTCAAAGTGAGTAATTGATATATATAAGAAAATTTGGTAACTTTATTTTATGTGTAAAGGCTAGATTTTGTGTAAGAATCGAGTTATGTTGGAGGCAATTTGAT

Coding sequence (CDS)

ATGGTGAGGATATTACCAATGGCTTCCACATTGCGACCTTCCATTTCGTCATTTAGGTTTGCTGCTGCCTCTCGTTTGGCTCATTCTCTCACTTCTTACACCCCTACTCGGAGGCTGCCTTCATTTCATCTCGGTTTTGCTGTTCCCCAGGCCCAATTCTTTGGTCTAAACACTTCGAAGTTGTGGCAAAAGGAGCATAATAACACCATAAGGGTGGCCATAAATGGATCTGTAGCACAGGCTAGCACAACAACCAGTCCAGAAAATATTCTTGAGTGGGTGAAAAAGGATAAGAGGAGATTTCTTCATGTTGTCTATCGTGTTGGAGATTTGGAGAGGACCATAAAATTCTACACTGAGTGCCTGGGTATGAAGCTGCTACGAAAACGTGACATACCAGAGGAAAGATATACAAATGCATTTCTTGGATACGGTCCTGAAGATGCTCACTTTGTTATTGAACTCACTTACAATTATGGAGTTGACAAGTATGATATTGGAACCGGATTCGGACATTTTGGTATTGCAGTTGAAGATGTTTCGAGGACGGTGGAACTTATAAAGGCCAAGGGTGGAAAGGTTACCAGAGAACCTGGTCCTGTTAAAGGTGGCAGTACTGTGATTGCCTTTATCGAAGATCCAGATGGCTATAAGTTTGAACTCTTGGAAAGGGGGCCTACTCCCGAGCCTTTATGTCAAGTAATGCTTCGTGTAGGTGATCTTGATCGATCCATAAATTTTTATGAGAAGGCTTTTGGCATGGAAGTCCTTCGCAAACGAGATAATCCAGATTATAAGTACACGATAGCAATGATGGGATATGGCCCTGAAGATAAAAATCCTGTACTGGAGTTGACTTACAACTATGGAGTTACTGACTACGAAAAAGGCAATGGTTATGCCCAGGTTTTTGTAGACAACATCGATTTTCTTAAGGAGTTGGAGTGA

Protein sequence

MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSKLWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDVSRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGYAQVFVDNIDFLKELE

Homology

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BLAST of Cp4.1LG04g10180 vs. ExPASy Swiss-Prot
Match: Q8W593 (Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g67280 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 4.8e-126
Identity = 227/316 (71.84%), Postives = 261/316 (82.59%), Query Frame = 0


Query: 1   MVRILPM-ASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTS 60
           MVRI+PM AS++RPS++   F+ + R   SL S   +R L        VPQ+Q FGL + 
Sbjct: 1   MVRIIPMAASSIRPSLAC--FSDSPRFPISLLSRNLSRTL-------HVPQSQLFGLTSH 60

Query: 61  KLWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYT 120
           KL ++   N + VA +G  AQA   T+ +++L WVK DKRR LHVVYRVGD++RTIKFYT
Sbjct: 61  KLLRRS-VNCLGVAESGKAAQA---TTQDDLLTWVKNDKRRMLHVVYRVGDMDRTIKFYT 120

Query: 121 ECLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVED 180
           ECLGMKLLRKRDIPEE+YTNAFLGYGPED+HFVIELTYNYGVDKYDIG GFGHFGIAV+D
Sbjct: 121 ECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGAGFGHFGIAVDD 180

Query: 181 VSRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVG 240
           V++TVEL+KAKGGKV+REPGPVKGG TVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVG
Sbjct: 181 VAKTVELVKAKGGKVSREPGPVKGGKTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVG 240

Query: 241 DLDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNG 300
           DLDR+I FYEKAFGME+LR RDNP+YKYTIAMMGYGPEDK PVLELTYNYGVT+Y+KGN 
Sbjct: 241 DLDRAIKFYEKAFGMELLRTRDNPEYKYTIAMMGYGPEDKFPVLELTYNYGVTEYDKGNA 300

Query: 301 YAQVFVDNIDFLKELE 316
           YAQ+ +   D  K  E
Sbjct: 301 YAQIAIGTDDVYKTAE 303

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy Swiss-Prot
Match: Q948T6 (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=1 SV=2)

HSP 1 Score: 369.4 bits (947), Expect = 4.1e-101
Identity = 172/231 (74.46%), Postives = 193/231 (83.55%), Query Frame = 0


Query: 86  SPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYG 145
           SPE +LEW KKDK+R LH VYRVGDL+RTIK YTEC GMKLLRKRD+PEE+YTNAFLG+G
Sbjct: 10  SPEVVLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFG 69

Query: 146 PEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDVSRTVELIKAK-GGKVTREPGPVKGG 205
           PED +F +ELTYNYGVDKYDIG GFGHF IA EDV +  E IK+    K+TREPGPVKGG
Sbjct: 70  PEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGG 129

Query: 206 STVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRSINFYEKAFGMEVLRKRDNPD 265
           STVIAF +DPDGY FEL++RGPTPEPLCQVMLRVGDLDRSI FYEKA GM++LRK+D PD
Sbjct: 130 STVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPD 189

Query: 266 YKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGYAQVFVDNIDFLKELE 316
           YKYTIAM+GY  EDK  V+ELTYNYGVT+Y KGN YAQV +   D  K  E
Sbjct: 190 YKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAE 240

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy Swiss-Prot
Match: O65398 (Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.1e-97
Identity = 162/227 (71.37%), Postives = 191/227 (84.14%), Query Frame = 0


Query: 89  NILEWVKKDKRRFLHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYGPED 148
           ++LEW KKD RRFLHVVYRVGDL+RTI+FYTE  GMKLLRKRDIPEE+Y+NAFLG+GPE 
Sbjct: 6   DLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPET 65

Query: 149 AHFVIELTYNYGVDKYDIGTGFGHFGIAVEDVSRTVELIKAKGGKVTREPGPVKGGSTVI 208
           ++FV+ELTYNYGV  YDIGTGFGHF I+ +DVS+ VE ++AKGG VTREPGPVKGG +VI
Sbjct: 66  SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125

Query: 209 AFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRSINFYEKAFGMEVLRKRDNPDYKYT 268
           AF++DPDGY FEL++RGPTPEP CQVMLRVGDLDR+I FYEKA GM +LRK + P+YKYT
Sbjct: 126 AFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYT 185

Query: 269 IAMMGYGPEDKNPVLELTYNYGVTDYEKGNGYAQVFVDNIDFLKELE 316
           I MMGY  E ++ VLELTYNY VT+Y KGN YAQ+ +   D  K  E
Sbjct: 186 IGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 232

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy Swiss-Prot
Match: Q39366 (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 PE=2 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.1e-95
Identity = 158/227 (69.60%), Postives = 192/227 (84.58%), Query Frame = 0


Query: 89  NILEWVKKDKRRFLHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYGPED 148
           +++EW KKDKRRFLHVVYRVGDL+RTI+FYTEC GMK+LRKRD+PEE+Y+NAFLG+GPE 
Sbjct: 6   DLVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPET 65

Query: 149 AHFVIELTYNYGVDKYDIGTGFGHFGIAVEDVSRTVELIKAKGGKVTREPGPVKGGSTVI 208
           ++FV+ELTYNYGV  YDIGTGFGHF I+ +DVS+ VE ++AKGG VTREPGPVKGG +VI
Sbjct: 66  SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVI 125

Query: 209 AFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRSINFYEKAFGMEVLRKRDNPDYKYT 268
           AF++DPDGY FEL++RGPTPEPLCQVMLRVGDLDR++ F EKA GM +LR+ + P+Y  T
Sbjct: 126 AFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-T 185

Query: 269 IAMMGYGPEDKNPVLELTYNYGVTDYEKGNGYAQVFVDNIDFLKELE 316
           I MMGY  E ++ VLELTYNYGVT+Y KGN YAQ+ +   D  K  E
Sbjct: 186 IGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAE 231

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy Swiss-Prot
Match: P0AC82 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 OX=83334 GN=gloA PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.7e-41
Identity = 76/124 (61.29%), Postives = 97/124 (78.23%), Query Frame = 0


Query: 100 RFLHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNY 159
           R LH + RVGDL+R+I FYT+ LGMKLLR  + PE +Y+ AF+GYGPE    VIELTYN+
Sbjct: 2   RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNW 61

Query: 160 GVDKYDIGTGFGHFGIAVEDVSRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKF 219
           GVDKY++GT +GH  ++V++ +   E I+  GG VTRE GPVKGG+TVIAF+EDPDGYK 
Sbjct: 62  GVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKI 121

Query: 220 ELLE 224
           EL+E
Sbjct: 122 ELIE 125

[Close]

BLAST of Cp4.1LG04g10180 vs. NCBI nr
Match: XP_023531878.1 (probable lactoylglutathione lyase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 626 bits (1614), Expect = 2.44e-225
Identity = 305/315 (96.83%), Postives = 307/315 (97.46%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK
Sbjct: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE
Sbjct: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. NCBI nr
Match: KAG6587981.1 (putative lactoylglutathione lyase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 622 bits (1605), Expect = 5.75e-224
Identity = 303/315 (96.19%), Postives = 307/315 (97.46%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMAS+LRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK
Sbjct: 1   MVRILPMASSLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           LWQKEHN+TIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE
Sbjct: 61  LWQKEHNDTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. NCBI nr
Match: KAG7021873.1 (putative lactoylglutathione lyase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 621 bits (1602), Expect = 1.65e-223
Identity = 302/315 (95.87%), Postives = 307/315 (97.46%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMAS+LRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK
Sbjct: 1   MVRILPMASSLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           LWQKEHN+TIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE
Sbjct: 61  LWQKEHNDTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGG+TVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. NCBI nr
Match: XP_022966265.1 (probable lactoylglutathione lyase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 619 bits (1595), Expect = 1.92e-222
Identity = 302/315 (95.87%), Postives = 305/315 (96.83%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMAS+LRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAV QAQFFGLNTSK
Sbjct: 1   MVRILPMASSLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVAQAQFFGLNTSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           LWQKEHNNTIRVAINGS AQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE
Sbjct: 61  LWQKEHNNTIRVAINGSGAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. NCBI nr
Match: XP_022929952.1 (probable lactoylglutathione lyase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 615 bits (1587), Expect = 3.18e-221
Identity = 300/315 (95.24%), Postives = 304/315 (96.51%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMAS+LRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGL TSK
Sbjct: 1   MVRILPMASSLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLKTSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           LWQKEHNNTIRVAINGSVAQASTTT PENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE
Sbjct: 61  LWQKEHNNTIRVAINGSVAQASTTTIPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGG+TVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGTTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSINFYEKAFGMEVLRKRD PDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSINFYEKAFGMEVLRKRDYPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy TrEMBL
Match: A0A6J1HR58 (Lactoylglutathione lyase OS=Cucurbita maxima OX=3661 GN=LOC111465976 PE=3 SV=1)

HSP 1 Score: 619 bits (1595), Expect = 9.30e-223
Identity = 302/315 (95.87%), Postives = 305/315 (96.83%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMAS+LRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAV QAQFFGLNTSK
Sbjct: 1   MVRILPMASSLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVAQAQFFGLNTSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           LWQKEHNNTIRVAINGS AQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE
Sbjct: 61  LWQKEHNNTIRVAINGSGAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy TrEMBL
Match: A0A6J1EP21 (Lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111436412 PE=3 SV=1)

HSP 1 Score: 615 bits (1587), Expect = 1.54e-221
Identity = 300/315 (95.24%), Postives = 304/315 (96.51%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMAS+LRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGL TSK
Sbjct: 1   MVRILPMASSLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLKTSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           LWQKEHNNTIRVAINGSVAQASTTT PENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE
Sbjct: 61  LWQKEHNNTIRVAINGSVAQASTTTIPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGG+TVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGTTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSINFYEKAFGMEVLRKRD PDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSINFYEKAFGMEVLRKRDYPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy TrEMBL
Match: A0A6J1CT38 (Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111014505 PE=3 SV=1)

HSP 1 Score: 573 bits (1477), Expect = 8.83e-205
Identity = 277/315 (87.94%), Postives = 294/315 (93.33%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPMAS+LRPSISSFRFAAASRLA SLTSY PTRRL SFHLGFAVPQAQ FGLN+SK
Sbjct: 1   MVRILPMASSLRPSISSFRFAAASRLARSLTSYAPTRRLASFHLGFAVPQAQLFGLNSSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           L +KEHNNT+RVA+NG  AQAST  SPEN+LEWVKKDKRR LHVVYRVGD+ERTIKFYTE
Sbjct: 61  LLKKEHNNTMRVAVNGYAAQASTKASPENLLEWVKKDKRRLLHVVYRVGDMERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDI EERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIA+EDV
Sbjct: 121 CLGMKLLRKRDISEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAIEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVML VG+
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLHVGN 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSI+FYEKAFGME+LRKRDNP+YKYTIAM+GYGPEDK+ VLELTYNYGVTDY+KGNGY
Sbjct: 241 LDRSIDFYEKAFGMELLRKRDNPEYKYTIAMLGYGPEDKHTVLELTYNYGVTDYDKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  K  E
Sbjct: 301 AQIAIGTDDVYKTAE 315

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy TrEMBL
Match: A0A1S3CHN8 (Lactoylglutathione lyase OS=Cucumis melo OX=3656 GN=LOC103501042 PE=3 SV=1)

HSP 1 Score: 564 bits (1453), Expect = 3.85e-201
Identity = 276/315 (87.62%), Postives = 289/315 (91.75%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTSK 60
           MVRILPM S LRPSISSF FAAASRL HSLTSYTPTR LPSF  GFAVPQAQFFGLN+SK
Sbjct: 1   MVRILPMPSFLRPSISSFTFAAASRLPHSLTSYTPTRSLPSFPFGFAVPQAQFFGLNSSK 60

Query: 61  LWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTE 120
           L +K  NNT+RV +NG  AQA TT SPENILEWVKKDKRR LHVVYRVGDLERTIKFYTE
Sbjct: 61  LLKK-CNNTMRVTVNGYAAQACTTASPENILEWVKKDKRRLLHVVYRVGDLERTIKFYTE 120

Query: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDV 180
           CLGMKLLRKRDIPEERYTNAFLGYGPE+AHFVIELTYNYGVDKYDIGTGFGHFG+AVEDV
Sbjct: 121 CLGMKLLRKRDIPEERYTNAFLGYGPEEAHFVIELTYNYGVDKYDIGTGFGHFGVAVEDV 180

Query: 181 SRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240
           SRTVELIKAKGGKVTREPGPVKGG+TVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD
Sbjct: 181 SRTVELIKAKGGKVTREPGPVKGGNTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGD 240

Query: 241 LDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGY 300
           LDRSI FYEKAFGME+LRKRDNP+YKYTIAM+GYGPEDKN VLELTYNYGVTDYEKGNGY
Sbjct: 241 LDRSIKFYEKAFGMELLRKRDNPEYKYTIAMLGYGPEDKNAVLELTYNYGVTDYEKGNGY 300

Query: 301 AQVFVDNIDFLKELE 315
           AQ+ +   D  +  E
Sbjct: 301 AQIAIGTDDVYRTAE 314

[Close]

BLAST of Cp4.1LG04g10180 vs. ExPASy TrEMBL
Match: A0A061GLJ1 (Lactoylglutathione lyase OS=Theobroma cacao OX=3641 GN=TCM_037825 PE=3 SV=1)

HSP 1 Score: 518 bits (1334), Expect = 1.03e-183
Identity = 255/318 (80.19%), Postives = 278/318 (87.42%), Query Frame = 0


Query: 1   MVRILPMASTLRPSISSFRFAA---ASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLN 60
           MVRI+PMAS++RPS+SSFRF+    ++R   SL+ +   RRL   HLG  VPQ QFFGL 
Sbjct: 1   MVRIIPMASSIRPSLSSFRFSGGVPSTRFGVSLSPFHLPRRLLFSHLGSTVPQLQFFGLK 60

Query: 61  TSKLWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKF 120
            SKL + E  + +   + G+V QAST  + E  LEWVKKDKRR LHVVYRVGDL+RTIKF
Sbjct: 61  ASKLLRAE-GSRVGTPLVGNVVQASTAAAEETALEWVKKDKRRMLHVVYRVGDLDRTIKF 120

Query: 121 YTECLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAV 180
           YTECLGMKLLRKRDIPEERYTNAFLGYGPED+HFVIELTYNYG+DKYDIGT FGHFGIAV
Sbjct: 121 YTECLGMKLLRKRDIPEERYTNAFLGYGPEDSHFVIELTYNYGIDKYDIGTAFGHFGIAV 180

Query: 181 EDVSRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLR 240
           EDV++TVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLR
Sbjct: 181 EDVAKTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLR 240

Query: 241 VGDLDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKG 300
           VGDL RSINFYEKAFGME+LR RDNP+YKYTIAMMGYGPEDKN VLELTYNYGVTDY+KG
Sbjct: 241 VGDLGRSINFYEKAFGMELLRTRDNPEYKYTIAMMGYGPEDKNAVLELTYNYGVTDYDKG 300

Query: 301 NGYAQVFVDNIDFLKELE 315
           N YAQVFVDNIDFLKELE
Sbjct: 301 NAYAQVFVDNIDFLKELE 317

[Close]

BLAST of Cp4.1LG04g10180 vs. TAIR 10
Match: AT1G67280.1 (Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily protein )

HSP 1 Score: 452.2 bits (1162), Expect = 3.4e-127
Identity = 227/316 (71.84%), Postives = 261/316 (82.59%), Query Frame = 0


Query: 1   MVRILPM-ASTLRPSISSFRFAAASRLAHSLTSYTPTRRLPSFHLGFAVPQAQFFGLNTS 60
           MVRI+PM AS++RPS++   F+ + R   SL S   +R L        VPQ+Q FGL + 
Sbjct: 1   MVRIIPMAASSIRPSLAC--FSDSPRFPISLLSRNLSRTL-------HVPQSQLFGLTSH 60

Query: 61  KLWQKEHNNTIRVAINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYT 120
           KL ++   N + VA +G  AQA   T+ +++L WVK DKRR LHVVYRVGD++RTIKFYT
Sbjct: 61  KLLRRS-VNCLGVAESGKAAQA---TTQDDLLTWVKNDKRRMLHVVYRVGDMDRTIKFYT 120

Query: 121 ECLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVED 180
           ECLGMKLLRKRDIPEE+YTNAFLGYGPED+HFVIELTYNYGVDKYDIG GFGHFGIAV+D
Sbjct: 121 ECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGVDKYDIGAGFGHFGIAVDD 180

Query: 181 VSRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVG 240
           V++TVEL+KAKGGKV+REPGPVKGG TVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVG
Sbjct: 181 VAKTVELVKAKGGKVSREPGPVKGGKTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVG 240

Query: 241 DLDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNG 300
           DLDR+I FYEKAFGME+LR RDNP+YKYTIAMMGYGPEDK PVLELTYNYGVT+Y+KGN 
Sbjct: 241 DLDRAIKFYEKAFGMELLRTRDNPEYKYTIAMMGYGPEDKFPVLELTYNYGVTEYDKGNA 300

Query: 301 YAQVFVDNIDFLKELE 316
           YAQ+ +   D  K  E
Sbjct: 301 YAQIAIGTDDVYKTAE 303

[Close]

BLAST of Cp4.1LG04g10180 vs. TAIR 10
Match: AT1G67280.2 (Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily protein )

HSP 1 Score: 399.8 bits (1026), Expect = 2.0e-111
Identity = 184/214 (85.98%), Postives = 200/214 (93.46%), Query Frame = 0


Query: 102 LHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYGPEDAHFVIELTYNYGV 161
           LHVVYRVGD++RTIKFYTECLGMKLLRKRDIPEE+YTNAFLGYGPED+HFVIELTYNYGV
Sbjct: 2   LHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDSHFVIELTYNYGV 61

Query: 162 DKYDIGTGFGHFGIAVEDVSRTVELIKAKGGKVTREPGPVKGGSTVIAFIEDPDGYKFEL 221
           DKYDIG GFGHFGIAV+DV++TVEL+KAKGGKV+REPGPVKGG TVIAFIEDPDGYKFEL
Sbjct: 62  DKYDIGAGFGHFGIAVDDVAKTVELVKAKGGKVSREPGPVKGGKTVIAFIEDPDGYKFEL 121

Query: 222 LERGPTPEPLCQVMLRVGDLDRSINFYEKAFGMEVLRKRDNPDYKYTIAMMGYGPEDKNP 281
           LERGPTPEPLCQVMLRVGDLDR+I FYEKAFGME+LR RDNP+YKYTIAMMGYGPEDK P
Sbjct: 122 LERGPTPEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPEYKYTIAMMGYGPEDKFP 181

Query: 282 VLELTYNYGVTDYEKGNGYAQVFVDNIDFLKELE 316
           VLELTYNYGVT+Y+KGN YAQ+ +   D  K  E
Sbjct: 182 VLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAE 215

[Close]

BLAST of Cp4.1LG04g10180 vs. TAIR 10
Match: AT1G11840.6 (glyoxalase I homolog )

HSP 1 Score: 355.9 bits (912), Expect = 3.3e-98
Identity = 164/243 (67.49%), Postives = 194/243 (79.84%), Query Frame = 0


Query: 73  AINGSVAQASTTTSPENILEWVKKDKRRFLHVVYRVGDLERTIKFYTECLGMKLLRKRDI 132
           A   S   +       ++LEW KKD RRFLHVVYRVGDL+RTI+FYTE  GMKLLRKRDI
Sbjct: 29  AAESSFLLSRNMAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDI 88

Query: 133 PEERYTNAFLGYGPEDAHFVIELTYNYGVDKYDIGTGFGHFGIAVEDVSRTVELIKAKGG 192
           PEE+Y+NAFLG+GPE ++FV+ELTYNYGV  YDIGTGFGHF I+ +DVS+ VE ++AKGG
Sbjct: 89  PEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGG 148

Query: 193 KVTREPGPVKGGSTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRSINFYEKAF 252
            VTREPGPVKGG +VIAF++DPDGY FEL++RGPTPEP CQVMLRVGDLDR+I FYEKA 
Sbjct: 149 NVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKAL 208

Query: 253 GMEVLRKRDNPDYKYTIAMMGYGPEDKNPVLELTYNYGVTDYEKGNGYAQVFVDNIDFLK 312
           GM +LRK + P+YKYTI MMGY  E ++ VLELTYNY VT+Y KGN YAQ+ +   D  K
Sbjct: 209 GMRLLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYK 268

Query: 313 ELE 316
             E
Sbjct: 269 SGE 271

[Close]

BLAST of Cp4.1LG04g10180 vs. TAIR 10
Match: AT1G11840.2 (glyoxalase I homolog )

HSP 1 Score: 355.5 bits (911), Expect = 4.3e-98
Identity = 162/227 (71.37%), Postives = 191/227 (84.14%), Query Frame = 0


Query: 89  NILEWVKKDKRRFLHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYGPED 148
           ++LEW KKD RRFLHVVYRVGDL+RTI+FYTE  GMKLLRKRDIPEE+Y+NAFLG+GPE 
Sbjct: 6   DLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPET 65

Query: 149 AHFVIELTYNYGVDKYDIGTGFGHFGIAVEDVSRTVELIKAKGGKVTREPGPVKGGSTVI 208
           ++FV+ELTYNYGV  YDIGTGFGHF I+ +DVS+ VE ++AKGG VTREPGPVKGG +VI
Sbjct: 66  SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125

Query: 209 AFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRSINFYEKAFGMEVLRKRDNPDYKYT 268
           AF++DPDGY FEL++RGPTPEP CQVMLRVGDLDR+I FYEKA GM +LRK + P+YKYT
Sbjct: 126 AFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYT 185

Query: 269 IAMMGYGPEDKNPVLELTYNYGVTDYEKGNGYAQVFVDNIDFLKELE 316
           I MMGY  E ++ VLELTYNY VT+Y KGN YAQ+ +   D  K  E
Sbjct: 186 IGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 232

[Close]

BLAST of Cp4.1LG04g10180 vs. TAIR 10
Match: AT1G11840.3 (glyoxalase I homolog )

HSP 1 Score: 355.5 bits (911), Expect = 4.3e-98
Identity = 162/227 (71.37%), Postives = 191/227 (84.14%), Query Frame = 0


Query: 89  NILEWVKKDKRRFLHVVYRVGDLERTIKFYTECLGMKLLRKRDIPEERYTNAFLGYGPED 148
           ++LEW KKD RRFLHVVYRVGDL+RTI+FYTE  GMKLLRKRDIPEE+Y+NAFLG+GPE 
Sbjct: 6   DLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPET 65

Query: 149 AHFVIELTYNYGVDKYDIGTGFGHFGIAVEDVSRTVELIKAKGGKVTREPGPVKGGSTVI 208
           ++FV+ELTYNYGV  YDIGTGFGHF I+ +DVS+ VE ++AKGG VTREPGPVKGG +VI
Sbjct: 66  SNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSVI 125

Query: 209 AFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRSINFYEKAFGMEVLRKRDNPDYKYT 268
           AF++DPDGY FEL++RGPTPEP CQVMLRVGDLDR+I FYEKA GM +LRK + P+YKYT
Sbjct: 126 AFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYT 185

Query: 269 IAMMGYGPEDKNPVLELTYNYGVTDYEKGNGYAQVFVDNIDFLKELE 316
           I MMGY  E ++ VLELTYNY VT+Y KGN YAQ+ +   D  K  E
Sbjct: 186 IGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 232

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g10180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W593	4.8e-126	71.84	Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q948T6	4.1e-101	74.46	Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=...	[more]
O65398	6.1e-97	71.37	Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1	[more]
Q39366	1.1e-95	69.60	Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 ...	[more]
P0AC82	4.7e-41	61.29	Lactoylglutathione lyase OS=Escherichia coli O157:H7 OX=83334 GN=gloA PE=3 SV=1	[more]

BLAST of Cp4.1LG04g10180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023531878.1	2.44e-225	96.83	probable lactoylglutathione lyase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
KAG6587981.1	5.75e-224	96.19	putative lactoylglutathione lyase, chloroplastic, partial [Cucurbita argyrosperm...	[more]
KAG7021873.1	1.65e-223	95.87	putative lactoylglutathione lyase, chloroplastic [Cucurbita argyrosperma subsp. ...	[more]
XP_022966265.1	1.92e-222	95.87	probable lactoylglutathione lyase, chloroplastic [Cucurbita maxima]	[more]
XP_022929952.1	3.18e-221	95.24	probable lactoylglutathione lyase, chloroplastic [Cucurbita moschata]	[more]

BLAST of Cp4.1LG04g10180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HR58	9.30e-223	95.87	Lactoylglutathione lyase OS=Cucurbita maxima OX=3661 GN=LOC111465976 PE=3 SV=1	[more]
A0A6J1EP21	1.54e-221	95.24	Lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111436412 PE=3 SV=1	[more]
A0A6J1CT38	8.83e-205	87.94	Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111014505 PE=3 SV=...	[more]
A0A1S3CHN8	3.85e-201	87.62	Lactoylglutathione lyase OS=Cucumis melo OX=3656 GN=LOC103501042 PE=3 SV=1	[more]
A0A061GLJ1	1.03e-183	80.19	Lactoylglutathione lyase OS=Theobroma cacao OX=3641 GN=TCM_037825 PE=3 SV=1	[more]

BLAST of Cp4.1LG04g10180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67280.1	3.4e-127	71.84	Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily protein	[more]
AT1G67280.2	2.0e-111	85.98	Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily protein	[more]
AT1G11840.6	3.3e-98	67.49	glyoxalase I homolog	[more]
AT1G11840.2	4.3e-98	71.37	glyoxalase I homolog	[more]
AT1G11840.3	4.3e-98	71.37	glyoxalase I homolog	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004361	Glyoxalase I	TIGRFAM	TIGR00068	TIGR00068	coord: 89..233 e-value: 2.7E-68 score: 226.1
IPR004360	Glyoxalase/fosfomycin resistance/dioxygenase domain	PFAM	PF00903	Glyoxalase	coord: 101..221 e-value: 1.7E-23 score: 83.2 coord: 233..302 e-value: 8.7E-8 score: 32.4
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 73..225 e-value: 3.5E-58 score: 197.9
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 228..315 e-value: 5.5E-29 score: 102.7
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 231..310
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 99..226
None	No IPR available	PANTHER	PTHR46036:SF14	LACTOYLGLUTATHIONE LYASE	coord: 27..312
None	No IPR available	PANTHER	PTHR46036	LACTOYLGLUTATHIONE LYASE	coord: 27..312
None	No IPR available	CDD	cd16358	GlxI_Ni	coord: 102..222 e-value: 9.41346E-79 score: 233.828
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00934	GLYOXALASE_I_1	coord: 103..124
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00934	GLYOXALASE_I_1	coord: 233..254
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00935	GLYOXALASE_I_2	coord: 167..179
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 230..315 score: 14.706601
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 100..224 score: 33.858818

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g10180.1	Cp4.1LG04g10180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0004462	lactoylglutathione lyase activity
molecular_function	GO:0046872	metal ion binding